Restriction map for
pFN21AA1789
(Circular) (Six-base) MAPSORT of: pFN21AA1789.seq Check: 699 from: 1 to: 6106
pFN21AA1789 6106 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3042 3057 4169 5553 5821 1632
Size: 1410 15 1112 1384 268 1917
Fragments arranged by size:
1917 1410 1384 1112 268 15
AflII C'TTAA_G
Cuts at: 820 1017 3269 820
Size: 197 2252 3657
Fragments arranged by size:
3657 2252 197
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2289 2321 2289
Size: 32 6074
AlwNI CAG_nnn'CTG
Cuts at: 2961 3849 5421 2961
Size: 888 1572 3646
Fragments arranged by size:
3646 1572 888
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2125 3415 3641 3793 5902 1493
Size: 632 1290 226 152 2109 1697
Fragments arranged by size:
2109 1697 1290 632 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 3431 1870
Size: 73 1488 4545
Fragments arranged by size:
4545 1488 73
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3427 4882 618
Size: 325 217 627 1640 1455 1842
Fragments arranged by size:
1842 1640 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3425 1391
Size: 2034 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2141 2393 928
Size: 971 242 252 4641
Fragments arranged by size:
4641 971 252 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4297 4331 1903
Size: 34 2360 34 3678
Fragments arranged by size:
3678 2360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2488 3998 5208 2488
Size: 1510 1210 3386
Fragments arranged by size:
3386 1510 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 4167 5323 1790
Size: 2377 1156 2573
Fragments arranged by size:
2573 2377 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3837 4795 442
Size: 416 53 83 399 24 2420 958 1753
Fragments arranged by size:
2420 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4766 1239
Size: 3527 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3007 4231 5096 5394 3007
Size: 1224 865 298 3719
Fragments arranged by size:
3719 1224 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3913 4295 4930 275
Size: 53 83 186 936 2380 382 635 1451
Fragments arranged by size:
2380 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2061 2322 4539 4937 5211 5358 5769 2061
Size: 261 2217 398 274 147 411 2398
Fragments arranged by size:
2398 2217 411 398 274 261 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2289 2319 1178
Size: 30 165 30 886 30 4965
Fragments arranged by size:
4965 886 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3251 1186
Size: 2065 4041
BseYI C'CCAG_C
Cuts at: 5309 5805 5309
Size: 496 5610
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2184 2201 2717 1163
Size: 573 448 17 516 4552
Fragments arranged by size:
4552 573 516 448 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4167 4252 5323 4167
Size: 85 1071 4950
Fragments arranged by size:
4950 1071 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3425 4167 4252 5323 1391
Size: 399 1635 742 85 1071 2174
Fragments arranged by size:
2174 1635 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2322 5769 2322
Size: 3447 2659
BspHI T'CATG_A
Cuts at: 2908 3995 5725 2908
Size: 1087 1730 3289
Fragments arranged by size:
3289 1730 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 3461 844
Size: 2233 384 3489
Fragments arranged by size:
3489 2233 384
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2364 2893 4601 4775 65
Size: 1183 1116 529 1708 174 1396
Fragments arranged by size:
1708 1396 1183 1116 529 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2452 3436 3803 4188 4205 5657 5743 5755
Size: 480 984 367 385 17 1452 86 12
Cuts at: 5755 6101 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 984 480 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 3622 4416 4436 3622
Size: 794 20 5292
Fragments arranged by size:
5292 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3553 4442 6071
Size: 54 1425 50 275 18 1723 889 1629
Cuts at: 6071 8
Size: 43
Fragments arranged by size:
1723 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 3061 3104 3363 4036 1353
Size: 870 838 43 259 673 3423
Fragments arranged by size:
3423 870 838 673 259 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3042 3057 4169 4766 5553 5821 1239
Size: 393 1410 15 1112 597 787 268 1524
Fragments arranged by size:
1524 1410 1112 787 597 393 268 15
EcoRI G'AATT_C
Cuts at: 2125 3415 3793 2125
Size: 1290 378 4438
Fragments arranged by size:
4438 1290 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2429 2693 2429
Size: 264 5842
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 5253 1678
Size: 3575 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2250 2282 2980 3012 3237
Size: 32 135 32 678 32 698 32 225
Cuts at: 3237 3269 3290 3322 4747 4779 4821 4853 1373
Size: 32 21 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 698 678 225 135 42 32
32 32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2948 3450 3696 669
Size: 988 281 1010 502 246 3079
Fragments arranged by size:
3079 1010 988 502 281 246
HindIII A'AGCT_T
Cuts at: 748 3266 748
Size: 2518 3588
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2790 5220 5404 1305
Size: 1485 2430 184 2007
Fragments arranged by size:
2430 2007 1485 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4284
Fragments arranged by size:
4284 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2693 4064 4423 4582 518
Size: 648 1011 516 1371 359 159 2042
Fragments arranged by size:
2042 1371 1011 648 516 359 159
MspA1I CmG'CkG
Cuts at: 1606 3522 4199 5347 5592 1606
Size: 1916 677 1148 245 2120
Fragments arranged by size:
2120 1916 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5554
NdeI CA'TA_TG
Cuts at: 387 2392 2656 387
Size: 2005 264 3837
Fragments arranged by size:
3837 2005 264
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2074 3464 5009 1132
Size: 97 845 1390 1545 2229
Fragments arranged by size:
2229 1545 1390 845 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2289 2321 4175 4207 5688 5720 2289
Size: 32 1854 32 1481 32 2675
Fragments arranged by size:
2675 1854 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3458 830
Size: 2628 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 2754 3454 4589 5270 5461 826
Size: 219 1709 700 1135 681 191 1471
Fragments arranged by size:
1709 1471 1135 700 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2986 3269 4210 5111 5373 820
Size: 197 926 1043 283 941 901 262 1553
Fragments arranged by size:
1553 1043 941 926 901 283 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 2347 4030 5
Size: 47 2295 1683 2081
Fragments arranged by size:
2295 2081 1683 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 3545 513
Size: 552 2480 3074
Fragments arranged by size:
3074 2480 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2431 4352 6064
Size: 274 80 33 51 1443 454 1921 1712
Cuts at: 6064 96
Size: 138
Fragments arranged by size:
1921 1712 1443 454 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6066 6098 6066
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI