Restriction map for
pFN21AA1782
(Circular) (Six-base) MAPSORT of: pFN21AA1782.seq Check: 2515 from: 1 to: 6319
pFN21AA1782 6319 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3116 3116
Size: 6319
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2352 4129 278
Size: 53 83 186 936 816 1777 2468
Fragments arranged by size:
2468 1777 936 816 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3069 3097 3662 1937
Size: 1132 28 565 4594
Fragments arranged by size:
4594 1132 565 28
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2721 2863 3508 4382 5766 6034 1632
Size: 1089 142 645 874 1384 268 1917
Fragments arranged by size:
1917 1384 1089 874 645 268 142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 3570 5150 3570
Size: 1580 4739
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 2409 3317 1166
Size: 1243 908 4168
Fragments arranged by size:
4168 1243 908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2339 3354 1184
Size: 1155 1015 4149
Fragments arranged by size:
4149 1155 1015
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2057
Size: 32 6287
AlwNI CAG_nnn'CTG
Cuts at: 4062 5634 4062
Size: 1572 4747
ApaI G_GGCC'C
Cuts at: 2086 2097 2086
Size: 11 6308
ApaLI G'TGCA_C
Cuts at: 2390 4376 5532 2390
Size: 1986 1156 3177
Fragments arranged by size:
3177 1986 1156
ApoI r'AATT_y
Cuts at: 1493 3578 3628 3854 4006 6115 1493
Size: 2085 50 226 152 2109 1697
Fragments arranged by size:
2109 2085 1697 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2911 3117 3177 3644 1870
Size: 73 135 833 206 60 467 4545
Fragments arranged by size:
4545 833 467 206 135 73 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3454 3487 3454
Size: 33 6286
BamHI G'GATC_C
Cuts at: 3113 3649 4016 3113
Size: 536 367 5416
Fragments arranged by size:
5416 536 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 2670 2755 3386 3640
Size: 325 217 627 654 229 85 631 254
Cuts at: 3640 5095 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 654 631 627 325 254 229
217 85
BanII G_rGCy'C
Cuts at: 1391 2086 2097 2268 3638 1391
Size: 695 11 171 1370 4072
Fragments arranged by size:
4072 1370 695 171 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2022 928
Size: 971 123 5225
Fragments arranged by size:
5225 971 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3286 3482 4211 5421 3286
Size: 196 729 1210 4184
Fragments arranged by size:
4184 1210 729 196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2097 2394 3389 4380 5536 1790
Size: 296 11 297 995 991 1156 2573
Fragments arranged by size:
2573 1156 995 991 297 296 11
BmgBI CAC'GTC
Cuts at: 1318 3490 1318
Size: 2172 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2081 4050 5008
Size: 416 53 83 399 24 664 1969 958
Cuts at: 5008 442
Size: 1753
Fragments arranged by size:
1969 1753 958 664 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 3179 2320
Size: 859 5460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2672 2768 3122 4979 1239
Size: 845 588 96 354 1857 2579
Fragments arranged by size:
2579 1857 845 588 354 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 4444 5309 5607 2324
Size: 2120 865 298 3036
Fragments arranged by size:
3036 2120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3522 4982 882
Size: 617 2023 1460 2219
Fragments arranged by size:
2219 2023 1460 617
BsaAI yAC'GTr
Cuts at: 493 1204 2741 4110 493
Size: 711 1537 1369 2702
Fragments arranged by size:
2702 1537 1369 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 4126 4508 5143
Size: 53 83 186 936 816 1777 382 635
Cuts at: 5143 275
Size: 1451
Fragments arranged by size:
1777 1451 936 816 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3570 4752 5150 5424 5571 5982 3570
Size: 1182 398 274 147 411 3907
Fragments arranged by size:
3907 1182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 2564 2594 1178
Size: 30 165 30 654 30 477 30 4903
Fragments arranged by size:
4903 654 477 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2572 2669 3155 1186
Size: 1012 374 97 486 4350
Fragments arranged by size:
4350 1012 486 374 97
BseYI C'CCAG_C
Cuts at: 2200 3209 3300 5522 6018 2200
Size: 1009 91 2222 496 2501
Fragments arranged by size:
2501 2222 1009 496 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2471 2756 3015 1163
Size: 573 735 285 259 4467
Fragments arranged by size:
4467 735 573 285 259
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3769 4530 5558 1540
Size: 275 186 805 963 761 1028 2301
Fragments arranged by size:
2301 1028 963 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 2385 2394 2661 3465 4380 4465 5536 2108
Size: 277 9 267 804 915 85 1071 2891
Fragments arranged by size:
2891 1071 915 804 277 267 85 9
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2097 2108 2268 2385 2394 2661
Size: 399 296 11 11 160 117 9 267
Cuts at: 2661 3389 3465 3638 4380 4465 5536 1391
Size: 728 76 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 728 399 296 267 173
160 117 85 76 11 11 9
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 3116 3674 844
Size: 1631 641 558 3489
Fragments arranged by size:
3489 1631 641 558
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 2139 2215 2668 2788 4206 1394
Size: 429 208 108 76 453 120 1418 3507
Fragments arranged by size:
3507 1418 453 429 208 120 108 76
BsrDI GCAATG_nn'
Cuts at: 65 1248 2029 2425 3220 4814 4988 65
Size: 1183 781 396 795 1594 174 1396
Fragments arranged by size:
1594 1396 1183 795 781 396 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2538 3171 3327 3570 4963 5150 1700
Size: 110 728 633 156 243 1393 187 2869
Fragments arranged by size:
2869 1393 728 633 243 187 156 110
BsrGI T'GTAC_A
Cuts at: 96 2172 96
Size: 2076 4243
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3219 794
Size: 2425 3894
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 2558 1184
Size: 1374 4945
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 3319 3322 2164
Size: 1155 3 5161
Fragments arranged by size:
5161 1155 3
BstYI r'GATC_y
Cuts at: 1972 3113 3649 4016 4401 4418 5870 5956 5968
Size: 1141 536 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1141 536 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3138 3285 3138
Size: 147 6172
BtgI C'CryG_G
Cuts at: 513 1065 2004 3517 513
Size: 552 939 1513 3315
Fragments arranged by size:
3315 1513 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2193 487
Size: 683 56 967 4613
Fragments arranged by size:
4613 967 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 3835 4629 4649 3463
Size: 372 794 20 5133
Fragments arranged by size:
5133 794 372 20
ClaI AT'CG_AT
Cuts at: 1554 2802 1554
Size: 1248 5071
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2419 2454 3532 3567 529
Size: 35 1855 35 1078 35 3281
Fragments arranged by size:
3281 1855 1078 35 35 35
DraI TTT'AAA
Cuts at: 2509 3623 2509
Size: 1114 5205
DraIII CAC_nnn'GTG
Cuts at: 2147 2147
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2623 2719
Size: 54 1425 50 275 18 196 597 96
Cuts at: 2719 2789 2811 3766 4655 6284 8
Size: 70 22 955 889 1629 43
Fragments arranged by size:
1629 1425 955 889 597 275 196 96
70 54 50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2131 3482 3589 4249 1353
Size: 778 1351 107 660 3423
Fragments arranged by size:
3423 1351 778 660 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3005 4906 5280 5426 387
Size: 1064 138 1416 1901 374 146 1280
Fragments arranged by size:
1901 1416 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2672 2721 2768 2863 3122 3508
Size: 393 452 588 49 47 95 259 386
Cuts at: 3508 4382 4979 5766 6034 1239
Size: 874 597 787 268 1524
Fragments arranged by size:
1524 874 787 597 588 452 393 386
268 259 95 49 47
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 3284 2225
Size: 1059 5260
EcoO109I rG'GnC_Cy
Cuts at: 2082 2083 2093 2094 2418 3134 3142 3158 3182
Size: 1 10 1 324 716 8 16 24
Cuts at: 3182 3290 3355 3395 2082
Size: 108 65 40 5006
Fragments arranged by size:
5006 716 324 108 65 40 24 16
10 8 1 1
EcoRI G'AATT_C
Cuts at: 3578 3628 4006 3578
Size: 50 378 5891
Fragments arranged by size:
5891 378 50
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 5466 1678
Size: 3788 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2243 2275 2894
Size: 32 135 32 599 32 40 32 619
Cuts at: 2894 2926 4960 4992 5034 5066 1373
Size: 32 2034 32 42 32 2626
Fragments arranged by size:
2626 2034 619 599 135 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3098 3663 3909 669
Size: 988 281 1160 565 246 3079
Fragments arranged by size:
3079 1160 988 565 281 246
HindIII A'AGCT_T
Cuts at: 748 2709 748
Size: 1961 4358
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3031 3918 3031
Size: 887 5432
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2721 2813 10
Size: 54 1425 343 889 92 3516
Fragments arranged by size:
3516 1425 889 343 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2409 3077 3317 3320 4277 4636
Size: 648 996 247 668 240 3 957 359
Cuts at: 4636 4795 518
Size: 159 2042
Fragments arranged by size:
2042 996 957 668 648 359 247 240
159 3
MspA1I CmG'CkG
Cuts at: 1606 3735 4412 5560 5805 1606
Size: 2129 677 1148 245 2120
Fragments arranged by size:
2129 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2540 3329 2540
Size: 789 5530
NcoI C'CATG_G
Cuts at: 513 1065 2004 3517 513
Size: 552 939 1513 3315
Fragments arranged by size:
3315 1513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2538 3327 2538
Size: 789 5530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3677 5222 1132
Size: 97 2448 1545 2229
Fragments arranged by size:
2448 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2269 2309 3197 1348
Size: 921 40 888 4470
Fragments arranged by size:
4470 921 888 40
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2713 1108
Size: 1605 4714
PfoI T'CCnGG_A
Cuts at: 2220 3109 3480 2220
Size: 889 371 5059
Fragments arranged by size:
5059 889 371
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2057 2089 4388 4420 5901 5933 2057
Size: 32 2299 32 1481 32 2443
Fragments arranged by size:
2443 2299 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3134 3142 3158 3290 3355 3134
Size: 8 16 132 65 6098
Fragments arranged by size:
6098 132 65 16 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2082 2093 2082
Size: 11 6308
PstI C_TGCA'G
Cuts at: 830 3671 830
Size: 2841 3478
PvuI CG_AT'CG
Cuts at: 2001 2806 2001
Size: 805 5514
SalI G'TCGA_C
Cuts at: 1936 3096 3661 1936
Size: 1160 565 4594
Fragments arranged by size:
4594 1160 565
SanDI GG'GwC_CC
Cuts at: 3290 3290
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 2725 2725
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 2684 3667 4802 5483 5674 826
Size: 219 1639 983 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 983 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 2538 1700
Size: 838 5481
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 4423 5324 5586 820
Size: 197 926 396 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 3449 1517
Size: 1932 4387
StyI C'CwwG_G
Cuts at: 513 1065 2004 3402 3517 3758 513
Size: 552 939 1398 115 241 3074
Fragments arranged by size:
3074 1398 939 552 241 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 2981 3040 3089 4346 4363 4516
Size: 1255 888 513 59 49 1257 17 153
Cuts at: 4516 4701 5146 325
Size: 185 445 1498
Fragments arranged by size:
1498 1257 1255 888 513 445 185 153
59 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 4565 6277
Size: 274 80 33 51 1443 195 2393 1712
Cuts at: 6277 96
Size: 138
Fragments arranged by size:
2393 1712 1443 274 195 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2364 2396 2564 2596 6279 6311 2364
Size: 32 168 32 3683 32 2372
Fragments arranged by size:
3683 2372 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3488 1316
Size: 2172 4147
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2714 3319 2383
Size: 331 605 5383
Fragments arranged by size:
5383 605 331
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 2072 3411 3449 4448 2072
Size: 1339 38 999 3943
Fragments arranged by size:
3943 1339 999 38
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2350 4127 276
Size: 53 83 186 936 816 1777 2468
Fragments arranged by size:
2468 1777 936 816 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI