Restriction map for
pFN21AA1779
(Circular) (Six-base) MAPSORT of: pFN21AA1779.seq Check: 5170 from: 1 to: 8335
pFN21AA1779 8335 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4396 4396
Size: 8335
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6145 278
Size: 53 83 186 936 4609 2468
Fragments arranged by size:
4609 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5678 1937
Size: 3741 4594
Acc65I G'GTAC_C
Cuts at: 5392 5656 5392
Size: 264 8071
AclI AA'CG_TT
Cuts at: 1563 6462 6835 1563
Size: 4899 373 3063
Fragments arranged by size:
4899 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2355 2967 3689 4133 4698 4802 6398
Size: 549 174 612 722 444 565 104 1596
Cuts at: 6398 7782 8050 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1596 1384 722 612 565 549 444
268 174 104
AfeI AGC'GCT
Cuts at: 4996 5050 4996
Size: 54 8281
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8138
AflIII A'CryG_T
Cuts at: 1203 4039 4869 7234 8211 1203
Size: 2836 830 2365 977 1327
Fragments arranged by size:
2836 2365 1327 977 830
AgeI A'CCGG_T
Cuts at: 4937 7166 4937
Size: 2229 6106
AhdI GACnn_n'nnGTC
Cuts at: 7064 7064
Size: 8335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3411 4351 1184
Size: 2227 940 5168
Fragments arranged by size:
5168 2227 940
AlwNI CAG_nnn'CTG
Cuts at: 2911 3874 4058 5617 6078 7650 2911
Size: 963 184 1559 461 1572 3596
Fragments arranged by size:
3596 1572 1559 963 461 184
ApaLI G'TGCA_C
Cuts at: 2438 4941 6392 7548 2438
Size: 2503 1451 1156 3225
Fragments arranged by size:
3225 2503 1451 1156
ApoI r'AATT_y
Cuts at: 1493 3225 4031 4106 5644 5870 6022 8131 1493
Size: 1732 806 75 1538 226 152 2109 1697
Fragments arranged by size:
2109 1732 1697 1538 806 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 6889 160
Size: 872 5857 1606
Fragments arranged by size:
5857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2090 4369 5660 1870
Size: 73 87 60 2279 1291 4545
Fragments arranged by size:
4545 2279 1291 87 73 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2388 2421 2388
Size: 33 8302
BamHI G'GATC_C
Cuts at: 2643 2805 5665 6032 2643
Size: 162 2860 367 4946
Fragments arranged by size:
4946 2860 367 162
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3345 4399 4767 5392 5656
Size: 325 217 627 1558 1054 368 625 264
Cuts at: 5656 7111 618
Size: 1455 1842
Fragments arranged by size:
1842 1558 1455 1054 627 625 368 325
264 217
BanII G_rGCy'C
Cuts at: 1391 2090 2563 3146 3591 3865 3990 4050 4376
Size: 699 473 583 445 274 125 60 326
Cuts at: 4376 5340 5654 1391
Size: 964 314 4072
Fragments arranged by size:
4072 964 699 583 473 445 326 314
274 125 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2487 2664 3750 5433 5631 928
Size: 971 588 177 1086 1683 198 3632
Fragments arranged by size:
3632 1683 1086 971 588 198 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3116 3150 6526 6560 1903
Size: 34 50 34 1095 34 3376 34 3678
Fragments arranged by size:
3678 3376 1095 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4555 6227 7437 4555
Size: 1672 1210 5453
Fragments arranged by size:
5453 1672 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6946 136
Size: 107 122 71 631 605 5274 1525
Fragments arranged by size:
5274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8330 8330
Size: 8335
BlpI GC'TnA_GC
Cuts at: 2562 3890 5752 2562
Size: 1328 1862 5145
Fragments arranged by size:
5145 1862 1328
Bme1580I G_kGCm'C
Cuts at: 1790 2442 3348 4404 4770 4877 4945 6396 7552
Size: 652 906 1056 366 107 68 1451 1156
Cuts at: 7552 1790
Size: 2573
Fragments arranged by size:
2573 1451 1156 1056 906 652 366 107
68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2804 5461 6066
Size: 416 53 83 399 24 1387 2657 605
Cuts at: 6066 7024 442
Size: 958 1753
Fragments arranged by size:
2657 1753 1387 958 605 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4713 4713
Size: 8335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3115 3313 4833 5365 6995 1239
Size: 1876 198 1520 532 1630 2579
Fragments arranged by size:
2579 1876 1630 1520 532 198
Bpu10I CC'TnA_GC
Cuts at: 2046 2930 5367 5499 2046
Size: 884 2437 132 4882
Fragments arranged by size:
4882 2437 884 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 2508 5520 6460 7325 7623 2369
Size: 139 3012 940 865 298 3081
Fragments arranged by size:
3081 3012 940 865 298 139
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6998 882
Size: 617 5499 2219
Fragments arranged by size:
5499 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3256 4042 4872 6126 493
Size: 711 2052 786 830 1254 2702
Fragments arranged by size:
2702 2052 1254 830 786 711
BsaBI GATnn'nnATC
Cuts at: 8329 8329
Size: 8335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2492 6142 6524 7159
Size: 53 83 186 936 959 3650 382 635
Cuts at: 7159 275
Size: 1451
Fragments arranged by size:
3650 1451 959 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2620 2691 4489 4937 6768 7166 7440 7587 7998
Size: 71 1798 448 1831 398 274 147 411
Cuts at: 7998 2620
Size: 2957
Fragments arranged by size:
2957 1831 1798 448 411 398 274 147
71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4730 4760 1178
Size: 30 165 30 3327 30 4753
Fragments arranged by size:
4753 3327 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 2790 3316 3371 3724 3870 3873 4345
Size: 1180 424 526 55 353 146 3 472
Cuts at: 4345 4751 5504 1186
Size: 406 753 4017
Fragments arranged by size:
4017 1180 753 526 472 424 406 353
146 55 3
BseYI C'CCAG_C
Cuts at: 2730 2890 3802 4403 4426 4441 4537 5126 5180
Size: 160 912 601 23 15 96 589 54
Cuts at: 5180 7538 8034 2730
Size: 2358 496 3031
Fragments arranged by size:
3031 2358 912 601 589 496 160 96
54 23 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2586 2924 4470 1163
Size: 573 850 338 1546 5028
Fragments arranged by size:
5028 1546 850 573 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4220 4224 5785 6546 7574 1540
Size: 275 186 2219 4 1561 761 1028 2301
Fragments arranged by size:
2301 2219 1561 1028 761 275 186 4
BsiHKAI G_wGCw'C
Cuts at: 2320 2442 2563 2620 3618 3865 4945 6396 6481
Size: 122 121 57 998 247 1080 1451 85
Cuts at: 6481 7552 2320
Size: 1071 3103
Fragments arranged by size:
3103 1451 1080 1071 998 247 122 121
85 57
BsmI GAATG_Cn'
Cuts at: 5373 5846 5939 5373
Size: 473 93 7769
Fragments arranged by size:
7769 473 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2029 2206 3353 2029
Size: 177 1147 7011
Fragments arranged by size:
7011 1147 177
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2320 2442 2563 2620 3146 3348
Size: 399 300 230 122 121 57 526 202
Cuts at: 3348 3591 3618 3865 3990 4050 4376 4404 4770
Size: 243 27 247 125 60 326 28 366
Cuts at: 4770 4877 4945 5340 5654 6396 6481 7552 1391
Size: 107 68 395 314 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 395 366 326
314 300 247 243 230 202 125 122
121 107 85 68 60 57 28 27
BspEI T'CCGG_A
Cuts at: 2691 7998 2691
Size: 5307 3028
BspHI T'CATG_A
Cuts at: 5379 6224 7954 5379
Size: 845 1730 5760
Fragments arranged by size:
5760 1730 845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2520 2946 3379 4396 5690 844
Size: 1676 426 433 1017 1294 3489
Fragments arranged by size:
3489 1676 1294 1017 433 426
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 6222 1394
Size: 429 257 4142 3507
Fragments arranged by size:
4142 3507 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 4692 6830 7004 65
Size: 1183 3444 2138 174 1396
Fragments arranged by size:
3444 2138 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4223 4292 4937 6979 7166 1700
Size: 110 2413 69 645 2042 187 2869
Fragments arranged by size:
2869 2413 2042 645 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 2340 2716 3579 3713 96
Size: 2244 376 863 134 4718
Fragments arranged by size:
4718 2244 863 376 134
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8335
BssSI C'ACGA_G
Cuts at: 6395 7407 6395
Size: 1012 7323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8335
BstBI TT'CG_AA
Cuts at: 3136 7139 3136
Size: 4003 4332
BstEII G'GTnAC_C
Cuts at: 1184 2894 4956 5172 1184
Size: 1710 2062 216 4347
Fragments arranged by size:
4347 2062 1710 216
BstYI r'GATC_y
Cuts at: 1972 2643 2805 3870 4119 5665 6032 6417 6434
Size: 671 162 1065 249 1546 367 385 17
Cuts at: 6434 7886 7972 7984 8330 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1546 1452 1065 671 385 367 346
249 162 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2322 2904 2322
Size: 582 7753
BtgI C'CryG_G
Cuts at: 513 1065 2011 2647 3879 4347 4395 4950 5058
Size: 552 946 636 1232 468 48 555 108
Cuts at: 5058 5112 5166 513
Size: 54 54 3682
Fragments arranged by size:
3682 1232 946 636 555 552 468 108
54 54 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3304 3899 487
Size: 683 56 2078 595 4923
Fragments arranged by size:
4923 2078 683 595 56
BtsI GCAGTG_nn'
Cuts at: 2550 2886 4921 5851 6645 6665 2550
Size: 336 2035 930 794 20 4220
Fragments arranged by size:
4220 2035 930 794 336 20
ClaI AT'CG_AT
Cuts at: 1554 2924 1554
Size: 1370 6965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8300
DraI TTT'AAA
Cuts at: 4036 5639 4036
Size: 1603 6732
DraIII CAC_nnn'GTG
Cuts at: 3352 3352
Size: 8335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4595 7342 809
Size: 507 3279 2747 1802
Fragments arranged by size:
3279 2747 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 4221 5595
Size: 54 1425 50 275 18 528 1863 1374
Cuts at: 5595 5782 6671 8300 8
Size: 187 889 1629 43
Fragments arranged by size:
1863 1629 1425 1374 889 528 275 187
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4221 5782 1537
Size: 275 2409 1561 4090
Fragments arranged by size:
4090 2409 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3665 3983 4080 4456 4660 4818 5328 6265
Size: 2312 318 97 376 204 158 510 937
Cuts at: 6265 1353
Size: 3423
Fragments arranged by size:
3423 2312 937 510 376 318 204 158
97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2132 3383 6922 7296 7442 387
Size: 1064 138 543 1251 3539 374 146 1280
Fragments arranged by size:
3539 1280 1251 1064 543 374 146 138
EcoICRI GAG'CTC
Cuts at: 2561 3863 2561
Size: 1302 7033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2355 2967 3115 3313 3689 4133
Size: 393 549 174 612 148 198 376 444
Cuts at: 4133 4698 4802 4833 5365 6398 6995 7782 8050
Size: 565 104 31 532 1033 597 787 268
Cuts at: 8050 1239
Size: 1524
Fragments arranged by size:
1524 1033 787 612 597 565 549 532
444 393 376 268 198 174 148 104
31
EcoNI CCTnn'n_nnAGG
Cuts at: 2903 2903
Size: 8335
EcoO109I rG'GnC_Cy
Cuts at: 2056 2908 4411 2056
Size: 852 1503 5980
Fragments arranged by size:
5980 1503 852
EcoRI G'AATT_C
Cuts at: 5644 6022 5644
Size: 378 7957
EcoRV GAT'ATC
Cuts at: 4665 4665
Size: 8335
FspI TGC'GCA
Cuts at: 6841 6841
Size: 8335
HaeII r_GCGC'y
Cuts at: 1678 4998 5052 7482 1678
Size: 3320 54 2430 2531
Fragments arranged by size:
3320 2531 2430 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2290 2322 2455 2487 3039
Size: 32 135 32 718 32 133 32 552
Cuts at: 3039 3071 4104 4136 4961 4993 5518 5550 6976
Size: 32 1033 32 825 32 525 32 1426
Cuts at: 6976 7008 7050 7082 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1426 1033 825 718 552 525 135
133 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2509 5679 5925 669
Size: 988 281 571 3170 246 3079
Fragments arranged by size:
3170 3079 988 571 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8335
HpaI GTT'AAC
Cuts at: 5925 5925
Size: 8335
KpnI G_GTAC'C
Cuts at: 5396 5660 5396
Size: 264 8071
MfeI C'AATT_G
Cuts at: 5934 5934
Size: 8335
MluI A'CGCG_T
Cuts at: 8211 8211
Size: 8335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4718 7449 7633 1305
Size: 3413 2731 184 2007
Fragments arranged by size:
3413 2731 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 5985
Fragments arranged by size:
5985 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3884 5378 6293 6652 6811 518
Size: 648 2718 1494 915 359 159 2042
Fragments arranged by size:
2718 2042 1494 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2259 2600 3270 3294 3342 4055 4349
Size: 407 246 341 670 24 48 713 294
Cuts at: 4349 5585 5631 5751 6428 7576 7821 1606
Size: 1236 46 120 677 1148 245 2120
Fragments arranged by size:
2120 1236 1148 713 677 670 407 341
294 246 245 120 48 46 24
NaeI GCC'GGC
Cuts at: 4225 4225
Size: 8335
NcoI C'CATG_G
Cuts at: 513 1065 4395 5112 513
Size: 552 3330 717 3736
Fragments arranged by size:
3736 3330 717 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8335
NgoMIV G'CCGG_C
Cuts at: 4223 4223
Size: 8335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8335
NotI GC'GGCC_GC
Cuts at: 5782 5782
Size: 8335
NspI r_CATG'y
Cuts at: 1132 1229 2341 4006 5693 7238 1132
Size: 97 1112 1665 1687 1545 2229
Fragments arranged by size:
2229 1687 1665 1545 1112 97
PasI CC'CwG_GG
Cuts at: 1348 2059 2846 4965 1348
Size: 711 787 2119 4718
Fragments arranged by size:
4718 2119 787 711
PciI A'CATG_T
Cuts at: 7234 7234
Size: 8335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3833 1108
Size: 2725 5610
PmeI GTTT'AAAC
Cuts at: 5639 5639
Size: 8335
PmlI CAC'GTG
Cuts at: 3256 4042 4872 3256
Size: 786 830 6719
Fragments arranged by size:
6719 830 786
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 6404 6436 7917 7949 4280
Size: 32 2092 32 1481 32 4666
Fragments arranged by size:
4666 2092 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2908 2908
Size: 8335
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8335
PsiI TTA'TAA
Cuts at: 5905 5905
Size: 8335
PspXI vC'TCGA_Gb
Cuts at: 4369 4369
Size: 8335
PstI C_TGCA'G
Cuts at: 830 2106 2642 4232 5687 830
Size: 1276 536 1590 1455 3478
Fragments arranged by size:
3478 1590 1455 1276 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8335
PvuII CAG'CTG
Cuts at: 2600 5585 5631 2600
Size: 2985 46 5304
Fragments arranged by size:
5304 2985 46
SacI G_AGCT'C
Cuts at: 2563 3865 2563
Size: 1302 7033
SacII CC_GC'GG
Cuts at: 2014 4350 2014
Size: 2336 5999
SalI G'TCGA_C
Cuts at: 1936 5677 1936
Size: 3741 4594
SapI GCTCTTCn'nnn_
Cuts at: 3665 4080 5328 3665
Size: 415 1248 6672
Fragments arranged by size:
6672 1248 415
SbfI CC_TGCA'GG
Cuts at: 5687 5687
Size: 8335
ScaI AGT'ACT
Cuts at: 6583 8295 6583
Size: 1712 6623
SexAI A'CCwGG_T
Cuts at: 5388 5388
Size: 8335
SfcI C'TryA_G
Cuts at: 826 1045 2102 2638 4135 4228 5683 6818 7499
Size: 219 1057 536 1497 93 1455 1135 681
Cuts at: 7499 7690 826
Size: 191 1471
Fragments arranged by size:
1497 1471 1455 1135 1057 681 536 219
191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8335
SmaI CCC'GGG
Cuts at: 1872 2032 5662 1872
Size: 160 3630 4545
Fragments arranged by size:
4545 3630 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 2523 4369 5535 6439 7340
Size: 197 926 405 175 1846 1166 904 901
Cuts at: 7340 7602 820
Size: 262 1553
Fragments arranged by size:
1846 1553 1166 926 904 901 405 262
197 175
SnaBI TAC'GTA
Cuts at: 493 6126 493
Size: 5633 2702
SpeI A'CTAG_T
Cuts at: 152 5246 152
Size: 5094 3241
SphI G_CATG'C
Cuts at: 1132 5693 1132
Size: 4561 3774
SspI AAT'ATT
Cuts at: 5 52 4178 6259 5
Size: 47 4126 2081 2081
Fragments arranged by size:
4126 2081 2081 47
StuI AGG'CCT
Cuts at: 1517 4497 1517
Size: 2980 5355
StyI C'CwwG_G
Cuts at: 513 1065 4395 5112 5774 513
Size: 552 3330 717 662 3074
Fragments arranged by size:
3330 3074 717 662 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4091 6362 6379 6532 6717 7162 325
Size: 1255 2511 2271 17 153 185 445 1498
Fragments arranged by size:
2511 2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2340 2716 3035
Size: 274 80 33 51 1443 363 376 319
Cuts at: 3035 3579 3713 5416 6581 8293 96
Size: 544 134 1703 1165 1712 138
Fragments arranged by size:
1712 1703 1443 1165 544 376 363 319
274 138 134 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 3608 3640 4730 4762 8295 8327 2421
Size: 32 1155 32 1090 32 3533 32 2429
Fragments arranged by size:
3533 2429 1155 1090 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3653 4216 1316
Size: 2337 563 5435
Fragments arranged by size:
5435 2337 563
XbaI T'CTAG_A
Cuts at: 2233 5671 2233
Size: 3438 4897
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5474 5474
Size: 8335
XhoI C'TCGA_G
Cuts at: 1943 4369 1943
Size: 2426 5909
XmaI C'CCGG_G
Cuts at: 1870 2030 5660 1870
Size: 160 3630 4545
Fragments arranged by size:
4545 3630 160
XmnI GAAnn'nnTTC
Cuts at: 2778 5203 5316 6464 2778
Size: 2425 113 1148 4649
Fragments arranged by size:
4649 2425 1148 113
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6143 276
Size: 53 83 186 936 4609 2468
Fragments arranged by size:
4609 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PspOMI PsrI RsrII SanDI SfiI
SfoI SrfI SwaI