Restriction map for
pFN21AA1777
(Circular) (Six-base) MAPSORT of: pFN21AA1777.seq Check: 6962 from: 1 to: 7549
pFN21AA1777 7549 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2687 2687
Size: 7549
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3231 5359 278
Size: 53 83 186 936 1695 2128 2468
Fragments arranged by size:
2468 2128 1695 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2736 4892 1937
Size: 799 2156 4594
Fragments arranged by size:
4594 2156 799
Acc65I G'GTAC_C
Cuts at: 2417 4870 2417
Size: 2453 5096
AclI AA'CG_TT
Cuts at: 1563 2812 5676 6049 1563
Size: 1249 2864 373 3063
Fragments arranged by size:
3063 2864 1249 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 3813 4082 4223 5612 6996 7264 1632
Size: 675 1506 269 141 1389 1384 268 1917
Fragments arranged by size:
1917 1506 1389 1384 675 269 268 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7352
AflIII A'CryG_T
Cuts at: 1203 6448 7425 1203
Size: 5245 977 1327
Fragments arranged by size:
5245 1327 977
AgeI A'CCGG_T
Cuts at: 6380 6380
Size: 7549
AhdI GACnn_n'nnGTC
Cuts at: 6278 6278
Size: 7549
AleI CACnn'nnGTG
Cuts at: 1166 2209 2729 1166
Size: 1043 520 5986
Fragments arranged by size:
5986 1043 520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2227 1184
Size: 1043 6506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2481 2513 3741 3773 4295 4327 4425 4457 2481
Size: 32 1228 32 522 32 98 32 5573
Fragments arranged by size:
5573 1228 522 98 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2096 2138 4192 4345 5292 6864 2096
Size: 42 2054 153 947 1572 2781
Fragments arranged by size:
2781 2054 1572 947 153 42
ApaI G_GGCC'C
Cuts at: 2376 2376
Size: 7549
ApaLI G'TGCA_C
Cuts at: 2983 5606 6762 2983
Size: 2623 1156 3770
Fragments arranged by size:
3770 2623 1156
ApoI r'AATT_y
Cuts at: 1493 3014 3310 3712 4545 4648 4858 5084 5236
Size: 1521 296 402 833 103 210 226 152
Cuts at: 5236 7345 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1521 833 402 296 226 210
152 103
AseI AT'TA_AT
Cuts at: 160 1032 6103 160
Size: 872 5071 1606
Fragments arranged by size:
5071 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 2998 3139 4874 1870
Size: 73 432 623 141 1735 4545
Fragments arranged by size:
4545 1735 623 432 141 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2401 2434 2401
Size: 33 7516
BamHI G'GATC_C
Cuts at: 2973 4879 5246 2973
Size: 1906 367 5276
Fragments arranged by size:
5276 1906 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 2417 3695 4870 6325
Size: 325 217 627 601 29 1278 1175 1455
Cuts at: 6325 618
Size: 1842
Fragments arranged by size:
1842 1455 1278 1175 627 601 325 217
29
BanII G_rGCy'C
Cuts at: 1391 2309 2376 2586 3393 3452 4868 1391
Size: 918 67 210 807 59 1416 4072
Fragments arranged by size:
4072 1416 918 807 210 67 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2748 4229 928
Size: 971 849 1481 4248
Fragments arranged by size:
4248 1481 971 849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2640 2674 5740 5774 1903
Size: 34 703 34 3066 34 3678
Fragments arranged by size:
3678 3066 703 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5441 6651 5441
Size: 1210 6339
BclI T'GATC_A
Cuts at: 1548 2637 1548
Size: 1089 6460
BfrBI ATG'CAT
Cuts at: 3553 3553
Size: 7549
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3161 6160 136
Size: 107 122 71 631 605 1489 2999 1525
Fragments arranged by size:
2999 1525 1489 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3328 7544 3328
Size: 4216 3333
BlpI GC'TnA_GC
Cuts at: 4966 4966
Size: 7549
Bme1580I G_kGCm'C
Cuts at: 1790 2376 2987 4753 5610 6766 1790
Size: 586 611 1766 857 1156 2573
Fragments arranged by size:
2573 1766 1156 857 611 586
BmgBI CAC'GTC
Cuts at: 1318 2281 1318
Size: 963 6586
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5280 6238 442
Size: 416 53 83 399 24 3863 958 1753
Fragments arranged by size:
3863 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7549
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 7549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3743 3795 4047 4294 4385 6209 1239
Size: 2504 52 252 247 91 1824 2579
Fragments arranged by size:
2579 2504 1824 252 247 91 52
Bpu10I CC'TnA_GC
Cuts at: 2308 2308
Size: 7549
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 5674 6539 6837 4132
Size: 1542 865 298 4844
Fragments arranged by size:
4844 1542 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6212 882
Size: 617 4713 2219
Fragments arranged by size:
4713 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2740 5340 493
Size: 711 1536 2600 2702
Fragments arranged by size:
2702 2600 1536 711
BsaBI GATnn'nnATC
Cuts at: 4728 7543 4728
Size: 2815 4734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3228 5356 5738 6373
Size: 53 83 186 936 1695 2128 382 635
Cuts at: 6373 275
Size: 1451
Fragments arranged by size:
2128 1695 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5982 6380 6654 6801 7212 5982
Size: 398 274 147 411 6319
Fragments arranged by size:
6319 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 3196 3226 3741
Size: 30 165 30 657 30 1106 30 515
Cuts at: 3741 3771 4425 4455 1178
Size: 30 654 30 4272
Fragments arranged by size:
4272 1106 657 654 515 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2722 2829 3722 1186
Size: 991 545 107 893 5013
Fragments arranged by size:
5013 991 893 545 107
BseYI C'CCAG_C
Cuts at: 2045 2833 2919 3752 4389 4803 6752 7248 2045
Size: 788 86 833 637 414 1949 496 2346
Fragments arranged by size:
2346 1949 833 788 637 496 414 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 2809 2875 2897 3034 3879 4331
Size: 573 771 302 66 22 137 845 452
Cuts at: 4331 4348 4469 4503 1163
Size: 17 121 34 4209
Fragments arranged by size:
4209 845 771 573 452 302 137 121
66 34 22 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4999 5760 6788 1540
Size: 275 186 2998 761 1028 2301
Fragments arranged by size:
2998 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2280 2309 2987 3393 3452 3783 3996 5610 5695
Size: 29 678 406 59 331 213 1614 85
Cuts at: 5695 6766 2280
Size: 1071 3063
Fragments arranged by size:
3063 1614 1071 678 406 331 213 85
59 29
BsmI GAATG_Cn'
Cuts at: 3121 4410 5060 5153 3121
Size: 1289 650 93 5517
Fragments arranged by size:
5517 1289 650 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2287 2287
Size: 7549
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2309 2376 2586 2987 3393 3452
Size: 399 490 29 67 210 401 406 59
Cuts at: 3452 3783 3996 4753 4868 5610 5695 6766 1391
Size: 331 213 757 115 742 85 1071 2174
Fragments arranged by size:
2174 1071 757 742 490 406 401 399
331 213 210 115 85 67 59 29
BspEI T'CCGG_A
Cuts at: 7212 7212
Size: 7549
BspHI T'CATG_A
Cuts at: 3088 3393 5438 7168 3088
Size: 305 2045 1730 3469
Fragments arranged by size:
3469 2045 1730 305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2687 2831 2897 4904 844
Size: 1843 144 66 2007 3489
Fragments arranged by size:
3489 2007 1843 144 66
BsrBI CCG'CTC
Cuts at: 1394 1823 5436 1394
Size: 429 3613 3507
Fragments arranged by size:
3613 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2788 3126 6044 6218 65
Size: 1183 1540 338 2918 174 1396
Fragments arranged by size:
2918 1540 1396 1183 338 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4335 6193 6380 1700
Size: 110 2525 1858 187 2869
Fragments arranged by size:
2869 2525 1858 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7549
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7549
BssSI C'ACGA_G
Cuts at: 5609 6621 5609
Size: 1012 6537
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4345 4407 794
Size: 3551 62 3936
Fragments arranged by size:
3936 3551 62
BstBI TT'CG_AA
Cuts at: 6353 6353
Size: 7549
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7549
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2698 2731 3767 4272 2011
Size: 687 33 1036 505 5288
Fragments arranged by size:
5288 1036 687 505 33
BstYI r'GATC_y
Cuts at: 1972 2973 3328 4437 4879 5246 5631 5648 7100
Size: 1001 355 1109 442 367 385 17 1452
Cuts at: 7100 7186 7198 7544 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1109 1001 442 385 367 355
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2368 3493 3523 513
Size: 552 1303 1125 30 4539
Fragments arranged by size:
4539 1303 1125 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6810
Fragments arranged by size:
6810 683 56
BtsI GCAGTG_nn'
Cuts at: 2144 2524 4290 5065 5859 5879 2144
Size: 380 1766 775 794 20 3814
Fragments arranged by size:
3814 1766 794 775 380 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7549
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3817 3852 529
Size: 35 3253 35 4226
Fragments arranged by size:
4226 3253 35 35
DraI TTT'AAA
Cuts at: 3894 4853 3894
Size: 959 6590
DraIII CAC_nnn'GTG
Cuts at: 3695 3695
Size: 7549
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2770 3363 3821 6556 809
Size: 507 1454 593 458 2735 1802
Fragments arranged by size:
2735 1802 1454 593 507 458
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2721 3162 3634
Size: 54 1425 50 275 18 891 441 472
Cuts at: 3634 4996 5885 7514 8
Size: 1362 889 1629 43
Fragments arranged by size:
1629 1425 1362 891 889 472 441 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4996 1537
Size: 275 3184 4090
Fragments arranged by size:
4090 3184 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 2574 3090 3244 3893 4752 5479 1353
Size: 930 291 516 154 649 859 727 3423
Fragments arranged by size:
3423 930 859 727 649 516 291 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6136 6510 6656 387
Size: 1064 138 4547 374 146 1280
Fragments arranged by size:
4547 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2307 3391 3450 2307
Size: 1084 59 6406
Fragments arranged by size:
6406 1084 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 3743 3795 3813 4047 4082 4223
Size: 393 675 1436 52 18 234 35 141
Cuts at: 4223 4294 4385 5612 6209 6996 7264 1239
Size: 71 91 1227 597 787 268 1524
Fragments arranged by size:
1524 1436 1227 787 675 597 393 268
234 141 91 71 52 35 18
EcoO109I rG'GnC_Cy
Cuts at: 2853 3373 4011 2853
Size: 520 638 6391
Fragments arranged by size:
6391 638 520
EcoRI G'AATT_C
Cuts at: 3310 3712 4858 5236 3310
Size: 402 1146 378 5623
Fragments arranged by size:
5623 1146 402 378
FspI TGC'GCA
Cuts at: 2447 6055 2447
Size: 3608 3941
FspAI rTGC'GCAy
Cuts at: 2447 2447
Size: 7549
HaeII r_GCGC'y
Cuts at: 1678 3652 6696 1678
Size: 1974 3044 2531
Fragments arranged by size:
3044 2531 1974
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2134 2166 2291
Size: 32 135 32 488 32 42 32 125
Cuts at: 2291 2323 2855 2887 4020 4052 4467 4499 4721
Size: 32 532 32 1133 32 415 32 222
Cuts at: 4721 4753 6190 6222 6264 6296 1373
Size: 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 1133 532 488 415 222 135
125 42 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4893 5139 669
Size: 988 281 2955 246 3079
Fragments arranged by size:
3079 2955 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7549
HpaI GTT'AAC
Cuts at: 5139 5139
Size: 7549
KpnI G_GTAC'C
Cuts at: 2421 4874 2421
Size: 2453 5096
MfeI C'AATT_G
Cuts at: 5148 5148
Size: 7549
MluI A'CGCG_T
Cuts at: 7425 7425
Size: 7549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 3405 6663 6847 1305
Size: 741 1359 3258 184 2007
Fragments arranged by size:
3258 2007 1359 741 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3636 10
Size: 54 1425 343 1804 3923
Fragments arranged by size:
3923 1804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2209 2681 2729 3434 4179 4719 4725
Size: 648 1043 472 48 705 745 540 6
Cuts at: 4725 5507 5866 6025 518
Size: 782 359 159 2042
Fragments arranged by size:
2042 1043 782 745 705 648 540 472
359 159 48 6
MspA1I CmG'CkG
Cuts at: 1606 3354 4393 4567 4965 5642 6790 7035 1606
Size: 1748 1039 174 398 677 1148 245 2120
Fragments arranged by size:
2120 1748 1148 1039 677 398 245 174
NcoI C'CATG_G
Cuts at: 513 1065 2368 3523 513
Size: 552 1303 1155 4539
Fragments arranged by size:
4539 1303 1155 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7549
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7549
NotI GC'GGCC_GC
Cuts at: 4996 4996
Size: 7549
NsiI A_TGCA'T
Cuts at: 3555 3555
Size: 7549
NspI r_CATG'y
Cuts at: 1132 1229 4087 4907 6452 1132
Size: 97 2858 820 1545 2229
Fragments arranged by size:
2858 2229 1545 820 97
PasI CC'CwG_GG
Cuts at: 1348 3466 1348
Size: 2118 5431
PciI A'CATG_T
Cuts at: 6448 6448
Size: 7549
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 1108
Size: 1290 6259
PfoI T'CCnGG_A
Cuts at: 2759 2816 2976 3509 3996 4191 2759
Size: 57 160 533 487 195 6117
Fragments arranged by size:
6117 533 487 195 160 57
PmeI GTTT'AAAC
Cuts at: 4853 4853
Size: 7549
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3741 3773 4425 4457 5618 5650 7131 7163 3741
Size: 32 652 32 1161 32 1481 32 4127
Fragments arranged by size:
4127 1481 1161 652 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3373 4011 3373
Size: 638 6911
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7549
PsiI TTA'TAA
Cuts at: 5119 5119
Size: 7549
PspOMI G'GGCC_C
Cuts at: 2372 2372
Size: 7549
PstI C_TGCA'G
Cuts at: 830 2951 3372 4901 830
Size: 2121 421 1529 3478
Fragments arranged by size:
3478 2121 1529 421
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7549
PvuII CAG'CTG
Cuts at: 4393 4393
Size: 7549
SacI G_AGCT'C
Cuts at: 2309 3393 3452 2309
Size: 1084 59 6406
Fragments arranged by size:
6406 1084 59
SalI G'TCGA_C
Cuts at: 1936 4891 1936
Size: 2955 4594
SapI GCTCTTCn'nnn_
Cuts at: 2574 2574
Size: 7549
SbfI CC_TGCA'GG
Cuts at: 4901 4901
Size: 7549
ScaI AGT'ACT
Cuts at: 5797 7509 5797
Size: 1712 5837
SfcI C'TryA_G
Cuts at: 826 1045 2947 3368 4828 4897 6032 6713 6904
Size: 219 1902 421 1460 69 1135 681 191
Cuts at: 6904 826
Size: 1471
Fragments arranged by size:
1902 1471 1460 1135 681 421 219 191
69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7549
SmaI CCC'GGG
Cuts at: 1872 4876 1872
Size: 3004 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5653 6554 6816 820
Size: 197 926 2168 1542 901 262 1553
Fragments arranged by size:
2168 1553 1542 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5340 493
Size: 4847 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7549
SphI G_CATG'C
Cuts at: 1132 4907 1132
Size: 3775 3774
SspI AAT'ATT
Cuts at: 5 52 5473 5
Size: 47 5421 2081
Fragments arranged by size:
5421 2081 47
StuI AGG'CCT
Cuts at: 1517 2438 1517
Size: 921 6628
StyI C'CwwG_G
Cuts at: 513 1065 2368 3523 3742 4988 513
Size: 552 1303 1155 219 1246 3074
Fragments arranged by size:
3074 1303 1246 1155 552 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3137 4403 5576 5593 5746 5931 6376
Size: 1255 1557 1266 1173 17 153 185 445
Cuts at: 6376 325
Size: 1498
Fragments arranged by size:
1557 1498 1266 1255 1173 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2518 3135 3545
Size: 274 80 33 51 1443 541 617 410
Cuts at: 3545 5795 7507 96
Size: 2250 1712 138
Fragments arranged by size:
2250 1712 1443 617 541 410 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2859 2891 3196 3228 3610 3642 3790
Size: 32 455 32 305 32 382 32 148
Cuts at: 3790 3822 7509 7541 2372
Size: 32 3687 32 2380
Fragments arranged by size:
3687 2380 455 382 305 148 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3400 1316
Size: 2084 5465
XbaI T'CTAG_A
Cuts at: 4885 4885
Size: 7549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3215 3215
Size: 7549
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7549
XmaI C'CCGG_G
Cuts at: 1870 4874 1870
Size: 3004 4545
XmnI GAAnn'nnTTC
Cuts at: 2112 5678 2112
Size: 3566 3983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3229 5357 276
Size: 53 83 186 936 1695 2128 2468
Fragments arranged by size:
2468 2128 1695 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BsiWI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI