Restriction map for
pFN21AA1776
(Circular) (Six-base) MAPSORT of: pFN21AA1776.seq Check: 534 from: 1 to: 13132
pFN21AA1776 13132 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2075 2399 2961 3869 4139 4376 4445 4610 6872
Size: 324 562 908 270 237 69 165 2262
Cuts at: 6872 7681 9419 9746 2075
Size: 809 1738 327 5461
Fragments arranged by size:
5461 2262 1738 908 809 562 327 324
270 237 165 69
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4818 10942 278
Size: 53 83 186 936 3282 6124 2468
Fragments arranged by size:
6124 3282 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3354 4305 10475 1937
Size: 1417 951 6170 4594
Fragments arranged by size:
6170 4594 1417 951
Acc65I G'GTAC_C
Cuts at: 4406 5003 6156 8693 10453 4406
Size: 597 1153 2537 1760 7085
Fragments arranged by size:
7085 2537 1760 1153 597
AclI AA'CG_TT
Cuts at: 1563 11259 11632 1563
Size: 9696 373 3063
Fragments arranged by size:
9696 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4463 4466 9949 10035 10430 11195 12579 12847
Size: 2831 3 5483 86 395 765 1384 268
Cuts at: 12847 1632
Size: 1917
Fragments arranged by size:
5483 2831 1917 1384 765 395 268 86
3
AfeI AGC'GCT
Cuts at: 8188 8188
Size: 13132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12935
AflIII A'CryG_T
Cuts at: 1203 2134 5708 6747 6983 7488 12031 13008 1203
Size: 931 3574 1039 236 505 4543 977 1327
Fragments arranged by size:
4543 3574 1327 1039 977 931 505 236
AgeI A'CCGG_T
Cuts at: 5535 5994 11963 5535
Size: 459 5969 6704
Fragments arranged by size:
6704 5969 459
AhdI GACnn_n'nnGTC
Cuts at: 4936 11861 4936
Size: 6925 6207
AleI CACnn'nnGTG
Cuts at: 1166 3384 3848 4175 5874 6792 8409 9123 10269
Size: 2218 464 327 1699 918 1617 714 1146
Cuts at: 10269 1166
Size: 4029
Fragments arranged by size:
4029 2218 1699 1617 1146 918 714 464
327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5663 7837 1184
Size: 4479 2174 6479
Fragments arranged by size:
6479 4479 2174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4740 4772 4740
Size: 32 13100
AlwNI CAG_nnn'CTG
Cuts at: 2129 2976 3037 3052 3375 3799 3861 4009 7108
Size: 847 61 15 323 424 62 148 3099
Cuts at: 7108 7534 9333 10875 12447 2129
Size: 426 1799 1542 1572 2814
Fragments arranged by size:
3099 2814 1799 1572 1542 847 426 424
323 148 62 61 15
ApaI G_GGCC'C
Cuts at: 2039 2496 3188 8901 9006 9609 9697 2039
Size: 457 692 5713 105 603 88 5474
Fragments arranged by size:
5713 5474 692 603 457 105 88
ApaLI G'TGCA_C
Cuts at: 2305 2617 5507 7375 8572 11189 12345 2305
Size: 312 2890 1868 1197 2617 1156 3092
Fragments arranged by size:
3092 2890 2617 1868 1197 1156 312
ApoI r'AATT_y
Cuts at: 1493 3026 10441 10667 10819 12928 1493
Size: 1533 7415 226 152 2109 1697
Fragments arranged by size:
7415 2109 1697 1533 226 152
AscI GG'CGCG_CC
Cuts at: 2266 2266
Size: 13132
AseI AT'TA_AT
Cuts at: 160 1032 11686 160
Size: 872 10654 1606
Fragments arranged by size:
10654 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13132
AvaI C'yCGr_G
Cuts at: 1870 1943 2582 3012 3430 4517 4572 4633 4874
Size: 73 639 430 418 1087 55 61 241
Cuts at: 4874 6465 6549 6643 7126 7380 7847 8247 8664
Size: 1591 84 94 483 254 467 400 417
Cuts at: 8664 9300 9520 9818 10053 10457 1870
Size: 636 220 298 235 404 4545
Fragments arranged by size:
4545 1591 1087 639 636 483 467 430
418 417 404 400 298 254 241 235
220 94 84 73 61 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6148 6181 6148
Size: 33 13099
BamHI G'GATC_C
Cuts at: 3104 3171 4069 4870 8318 10212 10462 10829 3104
Size: 67 898 801 3448 1894 250 367 5407
Fragments arranged by size:
5407 3448 1894 898 801 367 250 67
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2387 2432 2589 2882 3407
Size: 325 217 627 600 45 157 293 525
Cuts at: 3407 3694 4151 4221 4406 4433 5003 5129 5405
Size: 287 457 70 185 27 570 126 276
Cuts at: 5405 5925 6156 6860 7952 8022 8086 8693 9032
Size: 520 231 704 1092 70 64 607 339
Cuts at: 9032 9401 9494 10453 11908 618
Size: 369 93 959 1455 1842
Fragments arranged by size:
1842 1455 1092 959 704 627 607 600
570 525 520 457 369 339 325 293
287 276 231 217 185 157 126 93
70 70 64 45 27
BanII G_rGCy'C
Cuts at: 1391 2039 2496 2810 3020 3188 3570 3782 3887
Size: 648 457 314 210 168 382 212 105
Cuts at: 3887 3966 4538 7764 7856 7935 8205 8564 8901
Size: 79 572 3226 92 79 270 359 337
Cuts at: 8901 9006 9018 9609 9697 10182 10451 1391
Size: 105 12 591 88 485 269 4072
Fragments arranged by size:
4072 3226 648 591 572 485 457 382
359 337 314 270 269 212 210 168
105 105 92 88 79 79 12
BbeI G_GCGC'C
Cuts at: 2886 3698 9405 2886
Size: 812 5707 6613
Fragments arranged by size:
6613 5707 812
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2874 6093 6696 8259 928
Size: 971 975 3219 603 1563 5801
Fragments arranged by size:
5801 3219 1563 975 971 603
BbvCI CC'TCA_GC
Cuts at: 7805 9081 7805
Size: 1276 11856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 5071 5105 6540 6574 7894
Size: 34 2348 34 752 34 1435 34 1320
Cuts at: 7894 7928 11323 11357 1903
Size: 34 3395 34 3678
Fragments arranged by size:
3678 3395 2348 1435 1320 752 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 11024 12234 11024
Size: 1210 11922
BclI T'GATC_A
Cuts at: 1548 9808 1548
Size: 8260 4872
BfrBI ATG'CAT
Cuts at: 5979 10261 5979
Size: 4282 8850
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 2117 2577
Size: 107 122 71 631 605 394 51 460
Cuts at: 2577 2706 2784 3075 3096 3454 3579 4263 5256
Size: 129 78 291 21 358 125 684 993
Cuts at: 5256 5430 5934 6780 7254 7704 7752 7830 7950
Size: 174 504 846 474 450 48 78 120
Cuts at: 7950 7998 8214 9063 9549 9624 9795 10375 11743
Size: 48 216 849 486 75 171 580 1368
Cuts at: 11743 136
Size: 1525
Fragments arranged by size:
1525 1368 993 849 846 684 631 605
580 504 486 474 460 450 394 358
291 216 174 171 129 125 122 120
107 78 78 75 71 51 48 48
21
BglII A'GATC_T
Cuts at: 4290 13127 4290
Size: 8837 4295
BlpI GC'TnA_GC
Cuts at: 3569 8778 9472 10549 3569
Size: 5209 694 1077 6152
Fragments arranged by size:
6152 5209 1077 694
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2309 2390 2496 2621 3188 3895 4192
Size: 249 270 81 106 125 567 707 297
Cuts at: 4192 4436 4573 4904 5132 5330 5511 6608 6644
Size: 244 137 331 228 198 181 1097 36
Cuts at: 6644 6776 7250 7379 8025 8091 8576 8901 9006
Size: 132 474 129 646 66 485 325 105
Cuts at: 9006 9059 9499 9507 9609 9697 9724 9945 10078
Size: 53 440 8 102 88 27 221 133
Cuts at: 10078 11193 12349 1790
Size: 1115 1156 2573
Fragments arranged by size:
2573 1156 1115 1097 707 646 567 485
474 440 331 325 297 270 249 244
228 221 198 181 137 133 132 129
125 106 105 102 88 81 66 53
36 27 8
BmgBI CAC'GTC
Cuts at: 1318 2135 3331 4177 5218 5506 5593 8257 1318
Size: 817 1196 846 1041 288 87 2664 6193
Fragments arranged by size:
6193 2664 1196 1041 846 817 288 87
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6353 9682 9691
Size: 416 53 83 399 24 4936 3329 9
Cuts at: 9691 10863 11821 442
Size: 1172 958 1753
Fragments arranged by size:
4936 3329 1753 1172 958 416 399 83
53 24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3901 10024 3901
Size: 6123 7009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2239 2940 4904 6506 9335 10196 10214
Size: 794 206 701 1964 1602 2829 861 18
Cuts at: 10214 10349 11792 1239
Size: 135 1443 2579
Fragments arranged by size:
2829 2579 1964 1602 1443 861 794 701
206 135 18
Bpu10I CC'TnA_GC
Cuts at: 2997 7805 9067 9081 9568 2997
Size: 4808 1262 14 487 6561
Fragments arranged by size:
6561 4808 1262 487 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 9900 10423 11257 12122 12420 9900
Size: 523 834 865 298 10612
Fragments arranged by size:
10612 865 834 523 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2916 8148 8940 11795 882
Size: 617 1417 5232 792 2855 2219
Fragments arranged by size:
5232 2855 2219 1417 792 617
BsaAI yAC'GTr
Cuts at: 493 1204 3850 10923 493
Size: 711 2646 7073 2702
Fragments arranged by size:
7073 2702 2646 711
BsaBI GATnn'nnATC
Cuts at: 13126 13126
Size: 13132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2402 2883 3695 3737
Size: 53 83 186 936 869 481 812 42
Cuts at: 3737 4338 4713 4815 8283 8298 9402 10275 10939
Size: 601 375 102 3468 15 1104 873 664
Cuts at: 10939 11321 11956 275
Size: 382 635 1451
Fragments arranged by size:
3468 1451 1104 936 873 869 812 664
635 601 481 382 375 186 102 83
53 42 15
BsaWI w'CCGG_w
Cuts at: 2550 3943 4806 5535 5994 8829 9190 11565 11963
Size: 1393 863 729 459 2835 361 2375 398
Cuts at: 11963 12237 12384 12795 2550
Size: 274 147 411 2887
Fragments arranged by size:
2887 2835 2375 1393 863 729 459 411
398 361 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2230 2260 2314
Size: 30 165 30 603 30 194 30 54
Cuts at: 2314 2344 4235 4265 5981 6011 6528 6558 6815
Size: 30 1891 30 1716 30 517 30 257
Cuts at: 6815 6845 8498 8528 9935 9965 1178
Size: 30 1653 30 1407 30 4345
Fragments arranged by size:
4345 1891 1716 1653 1407 603 517 257
194 165 54 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3000 7943 9332 10013 1186
Size: 1814 4943 1389 681 4305
Fragments arranged by size:
4943 4305 1814 1389 681
BseYI C'CCAG_C
Cuts at: 2100 2292 2333 2976 3045 3176 4670 4912 5803
Size: 192 41 643 69 131 1494 242 891
Cuts at: 5803 5937 6440 8072 8148 8858 8997 9435 9477
Size: 134 503 1632 76 710 139 438 42
Cuts at: 9477 9678 9932 10291 12335 12831 2100
Size: 201 254 359 2044 496 2401
Fragments arranged by size:
2401 2044 1632 1494 891 710 643 503
496 438 359 254 242 201 192 139
134 131 76 69 42 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2383 2667 4033 4153 4978 5065 5560
Size: 573 647 284 1366 120 825 87 495
Cuts at: 5560 5607 5623 6019 6028 6316 6372 7910 8272
Size: 47 16 396 9 288 56 1538 362
Cuts at: 8272 8289 8658 8737 9250 9310 10436 1163
Size: 17 369 79 513 60 1126 3859
Fragments arranged by size:
3859 1538 1366 1126 825 647 573 513
495 396 369 362 288 284 120 87
79 60 56 47 17 16 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8803 10187 10307 10582 11343 12371
Size: 275 186 6802 1384 120 275 761 1028
Cuts at: 12371 1540
Size: 2301
Fragments arranged by size:
6802 2301 1384 1028 761 275 275 186
120
BsiHKAI G_wGCw'C
Cuts at: 2309 2621 3570 4066 5357 5420 5444 5511 5994
Size: 312 949 496 1291 63 24 67 483
Cuts at: 5994 6801 7379 7764 7925 8084 8205 8396 8576
Size: 807 578 385 161 159 121 191 180
Cuts at: 8576 9018 9130 9473 10182 11193 11278 12349 2309
Size: 442 112 343 709 1011 85 1071 3092
Fragments arranged by size:
3092 1291 1071 1011 949 807 709 578
496 483 442 385 343 312 191 180
161 159 121 112 85 67 63 24
BsmI GAATG_Cn'
Cuts at: 3365 4316 4976 5483 5858 5981 6833 7339 8516
Size: 951 660 507 375 123 852 506 1177
Cuts at: 8516 8774 9107 9356 9545 10034 10643 10736 3365
Size: 258 333 249 189 489 609 93 5761
Fragments arranged by size:
5761 1177 951 852 660 609 507 506
489 375 333 258 249 189 123 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6482 6482
Size: 13132
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2309 2390 2496 2621 2810 3020
Size: 399 249 270 81 106 125 189 210
Cuts at: 3020 3188 3570 3782 3887 3895 3966 4066 4192
Size: 168 382 212 105 8 71 100 126
Cuts at: 4192 4436 4538 4573 4904 5132 5330 5357 5420
Size: 244 102 35 331 228 198 27 63
Cuts at: 5420 5444 5511 5994 6608 6644 6776 6801 7250
Size: 24 67 483 614 36 132 25 449
Cuts at: 7250 7379 7764 7856 7925 7935 8025 8084 8091
Size: 129 385 92 69 10 90 59 7
Cuts at: 8091 8205 8396 8564 8576 8901 9006 9018 9059
Size: 114 191 168 12 325 105 12 41
Cuts at: 9059 9130 9473 9499 9507 9609 9697 9724 9945
Size: 71 343 26 8 102 88 27 221
Cuts at: 9945 10078 10182 10451 11193 11278 12349 1391
Size: 133 104 269 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 483 449 399 385
382 343 331 325 270 269 249 244
228 221 212 210 198 191 189 168
168 133 132 129 126 125 114 106
105 105 104 102 102 100 92 90
88 85 81 71 71 69 67 63
59 41 36 35 27 27 26 25
24 12 12 10 8 8 7
BspEI T'CCGG_A
Cuts at: 3943 12795 3943
Size: 8852 4280
BspHI T'CATG_A
Cuts at: 8484 11021 12751 8484
Size: 2537 1730 8865
Fragments arranged by size:
8865 2537 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2075 2114 2399 2961 3869 4139 4376 4445
Size: 1231 39 285 562 908 270 237 69
Cuts at: 4445 4610 5015 6276 6872 7592 7681 7829 8501
Size: 165 405 1261 596 720 89 148 672
Cuts at: 8501 9218 9318 9341 9419 9746 10487 844
Size: 717 100 23 78 327 741 3489
Fragments arranged by size:
3489 1261 1231 908 741 720 717 672
596 562 405 327 285 270 237 165
148 100 89 78 69 39 23
BsrBI CCG'CTC
Cuts at: 1394 1823 11019 1394
Size: 429 9196 3507
Fragments arranged by size:
9196 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2815 3559 6301 7708 11627 11801 65
Size: 1183 1567 744 2742 1407 3919 174 1396
Fragments arranged by size:
3919 2742 1567 1407 1396 1183 744 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2838 2879 2930 3420 4355 4389 4722
Size: 110 1028 41 51 490 935 34 333
Cuts at: 4722 5429 5535 5994 7560 8585 8931 10059 10186
Size: 707 106 459 1566 1025 346 1128 127
Cuts at: 10186 10323 11776 11963 1700
Size: 137 1453 187 2869
Fragments arranged by size:
2869 1566 1453 1128 1028 1025 935 707
490 459 346 333 187 137 127 110
106 51 41 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 13132
BssHII G'CGCG_C
Cuts at: 1925 2266 1925
Size: 341 12791
BssSI C'ACGA_G
Cuts at: 2612 11192 12204 2612
Size: 8580 1012 3540
Fragments arranged by size:
8580 3540 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2952 3375 3624 3861 4167 6519 6936 8676
Size: 2158 423 249 237 306 2352 417 1740
Cuts at: 8676 9333 794
Size: 657 4593
Fragments arranged by size:
4593 2352 2158 1740 657 423 417 306
249 237
BstBI TT'CG_AA
Cuts at: 5955 8448 11936 5955
Size: 2493 3488 7151
Fragments arranged by size:
7151 3488 2493
BstEII G'GTnAC_C
Cuts at: 1184 4786 5657 1184
Size: 3602 871 8659
Fragments arranged by size:
8659 3602 871
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3115 3956 5495 6448 6923 8411 10389 2011
Size: 1104 841 1539 953 475 1488 1978 4754
Fragments arranged by size:
4754 1978 1539 1488 1104 953 841 475
BstYI r'GATC_y
Cuts at: 1972 3104 3171 4069 4290 4449 4870 7104 7185
Size: 1132 67 898 221 159 421 2234 81
Cuts at: 7185 7437 8318 10212 10462 10829 11214 11231 12683
Size: 252 881 1894 250 367 385 17 1452
Cuts at: 12683 12769 12781 13127 1972
Size: 86 12 346 1977
Fragments arranged by size:
2234 1977 1894 1452 1132 898 881 421
385 367 346 252 250 221 159 86
81 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 4074 4156 4717 4074
Size: 82 561 12489
Fragments arranged by size:
12489 561 82
BtgI C'CryG_G
Cuts at: 513 1065 2461 2704 3151 3382 3949 4035 4904
Size: 552 1396 243 447 231 567 86 869
Cuts at: 4904 5380 5755 7456 7504 8218 8817 9061 9499
Size: 476 375 1701 48 714 599 244 438
Cuts at: 9499 9574 10231 10270 10388 513
Size: 75 657 39 118 3257
Fragments arranged by size:
3257 1701 1396 869 714 657 599 567
552 476 447 438 375 244 243 231
118 86 75 48 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4620 4777 487
Size: 683 56 3394 157 8842
Fragments arranged by size:
8842 3394 683 157 56
BtsI GCAGTG_nn'
Cuts at: 2371 2957 3207 3415 4024 4665 5032 5328 5635
Size: 586 250 208 609 641 367 296 307
Cuts at: 5635 6140 6529 7735 7900 8956 9075 9505 10078
Size: 505 389 1206 165 1056 119 430 573
Cuts at: 10078 10648 11442 11462 2371
Size: 570 794 20 4041
Fragments arranged by size:
4041 1206 1056 794 641 609 586 573
570 505 430 389 367 307 296 250
208 165 119 20
ClaI AT'CG_AT
Cuts at: 1554 5346 1554
Size: 3792 9340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 13097
DraI TTT'AAA
Cuts at: 10436 10436
Size: 13132
DraIII CAC_nnn'GTG
Cuts at: 2439 3624 10093 2439
Size: 1185 6469 5478
Fragments arranged by size:
6469 5478 1185
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8792 12139 809
Size: 507 7476 3347 1802
Fragments arranged by size:
7476 3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2569 2932
Size: 54 1425 50 275 18 259 480 363
Cuts at: 2932 3067 3268 3703 3757 3826 4353 4942 5122
Size: 135 201 435 54 69 527 589 180
Cuts at: 5122 5319 5383 5688 6612 7045 7705 8215 8401
Size: 197 64 305 924 433 660 510 186
Cuts at: 8401 8800 8989 10061 10101 10184 10304 10579 11468
Size: 399 189 1072 40 83 120 275 889
Cuts at: 11468 13097 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1072 924 889 660 589 527
510 480 435 433 399 363 305 275
275 259 201 197 189 186 180 135
120 83 69 64 54 54 50 43
40 18
EagI C'GGCC_G
Cuts at: 1537 1812 8800 10184 10304 10579 1537
Size: 275 6988 1384 120 275 4090
Fragments arranged by size:
6988 4090 1384 275 275 120
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 5601 6947 11062 1353
Size: 1733 2515 1346 4115 3423
Fragments arranged by size:
4115 3423 2515 1733 1346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10312 11719 12093 12239 387
Size: 1064 138 8723 1407 374 146 1280
Fragments arranged by size:
8723 1407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3568 7762 8203 9016 10180 3568
Size: 4194 441 813 1164 6520
Fragments arranged by size:
6520 4194 1164 813 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2239 2940 4463 4466 4904 6506
Size: 393 401 206 701 1523 3 438 1602
Cuts at: 6506 9335 9949 10035 10196 10214 10349 10430 11195
Size: 2829 614 86 161 18 135 81 765
Cuts at: 11195 11792 12579 12847 1239
Size: 597 787 268 1524
Fragments arranged by size:
2829 1602 1524 1523 787 765 701 614
597 438 401 393 268 206 161 135
86 81 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2777 7745 8600 9314 2777
Size: 4968 855 714 6595
Fragments arranged by size:
6595 4968 855 714
EcoO109I rG'GnC_Cy
Cuts at: 2035 2036 2109 2126 2528 2558 2579 2659 2733
Size: 1 73 17 402 30 21 80 74
Cuts at: 2733 3119 3185 4699 5236 7123 7581 8897 8898
Size: 386 66 1514 537 1887 458 1316 1
Cuts at: 8898 9002 9003 9238 9330 9606 9814 10120 10310
Size: 104 1 235 92 276 208 306 190
Cuts at: 10310 10352 2035
Size: 42 4815
Fragments arranged by size:
4815 1887 1514 1316 537 458 402 386
306 276 235 208 190 104 92 80
74 73 66 42 30 21 17 1
1 1
EcoRI G'AATT_C
Cuts at: 3026 10441 10819 3026
Size: 7415 378 5339
Fragments arranged by size:
7415 5339 378
EcoRV GAT'ATC
Cuts at: 5089 7912 5089
Size: 2823 10309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5800 5800
Size: 13132
FseI GG_CCGG'CC
Cuts at: 10190 10190
Size: 13132
FspI TGC'GCA
Cuts at: 3854 6231 11638 3854
Size: 2377 5407 5348
Fragments arranged by size:
5407 5348 2377
HaeII r_GCGC'y
Cuts at: 1678 2859 2886 3698 8190 9405 9750 12279 1678
Size: 1181 27 812 4492 1215 345 2529 2531
Fragments arranged by size:
4492 2531 2529 1215 1181 812 345 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2314 2346 2826 2858 4610
Size: 32 135 32 742 32 480 32 1752
Cuts at: 4610 4642 4862 4894 4922 4954 5339 5371 6528
Size: 32 220 32 28 32 385 32 1157
Cuts at: 6528 6560 6815 6847 7907 7939 8378 8410 8498
Size: 32 255 32 1060 32 439 32 88
Cuts at: 8498 8530 9455 9487 11773 11805 11847 11879 1373
Size: 32 925 32 2286 32 42 32 2626
Fragments arranged by size:
2626 2286 1752 1157 1060 925 742 480
439 385 255 220 135 88 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 3397 3523 3631 4306 4663
Size: 988 281 859 600 126 108 675 357
Cuts at: 4663 5140 6745 8308 8797 8812 9391 10476 10722
Size: 477 1605 1563 489 15 579 1085 246
Cuts at: 10722 669
Size: 3079
Fragments arranged by size:
3079 1605 1563 1085 988 859 675 600
579 489 477 357 281 246 126 108
15
HindIII A'AGCT_T
Cuts at: 748 8094 748
Size: 7346 5786
HpaI GTT'AAC
Cuts at: 10722 10722
Size: 13132
KasI G'GCGC_C
Cuts at: 2882 3694 9401 2882
Size: 812 5707 6613
Fragments arranged by size:
6613 5707 812
KpnI G_GTAC'C
Cuts at: 4410 5007 6160 8697 10457 4410
Size: 597 1153 2537 1760 7085
Fragments arranged by size:
7085 2537 1760 1153 597
MfeI C'AATT_G
Cuts at: 10731 10731
Size: 13132
MluI A'CGCG_T
Cuts at: 13008 13008
Size: 13132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2812 4325 5842 12246 12430 1305
Size: 1507 1513 1517 6404 184 2007
Fragments arranged by size:
6404 2007 1517 1513 1507 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2091 2571 5385 5690 6614
Size: 54 1425 343 259 480 2814 305 924
Cuts at: 6614 7047 7707 8217 10103 10
Size: 433 660 510 1886 3039
Fragments arranged by size:
3039 2814 1886 1425 924 660 510 480
433 343 305 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3384 3749 3848 4175 5198 5216 5645
Size: 648 2218 365 99 327 1023 18 429
Cuts at: 5645 5874 6792 6944 8409 9123 9579 10269 11090
Size: 229 918 152 1465 714 456 690 821
Cuts at: 11090 11449 11608 518
Size: 359 159 2042
Fragments arranged by size:
2218 2042 1465 1023 918 821 714 690
648 456 429 365 359 327 229 159
152 99 18
MspA1I CmG'CkG
Cuts at: 1606 2100 2337 2463 2502 2602 2706 2790 2823
Size: 494 237 126 39 100 104 84 33
Cuts at: 2823 2913 2984 3049 3058 3273 3300 3516 3789
Size: 90 71 65 9 215 27 216 273
Cuts at: 3789 3995 4122 4248 4464 4683 4906 5242 5544
Size: 206 127 126 216 219 223 336 302
Cuts at: 5544 5664 5803 5919 6042 6162 6285 6414 6444
Size: 120 139 116 123 120 123 129 30
Cuts at: 6444 7239 7368 7724 7769 7863 8118 8328 8527
Size: 795 129 356 45 94 255 210 199
Cuts at: 8527 8544 8805 8819 9009 9165 9294 9417 9534
Size: 17 261 14 190 156 129 123 117
Cuts at: 9534 9615 9654 9660 9780 9876 10233 10291 10420
Size: 81 39 6 120 96 357 58 129
Cuts at: 10420 10548 11225 12373 12618 1606
Size: 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 795 677 494 357 356 336
302 273 261 255 245 237 223 219
216 216 215 210 206 199 190 156
139 129 129 129 129 128 127 126
126 123 123 123 120 120 120 117
116 104 100 96 94 90 84 81
71 65 58 45 39 39 33 30
27 17 14 9 6
NaeI GCC'GGC
Cuts at: 2881 2932 4357 4391 4724 5431 8587 10188 2881
Size: 51 1425 34 333 707 3156 1601 5825
Fragments arranged by size:
5825 3156 1601 1425 707 333 51 34
NarI GG'CG_CC
Cuts at: 2883 3695 9402 2883
Size: 812 5707 6613
Fragments arranged by size:
6613 5707 812
NcoI C'CATG_G
Cuts at: 513 1065 3151 5380 5755 7456 9061 9574 10388
Size: 552 2086 2229 375 1701 1605 513 814
Cuts at: 10388 513
Size: 3257
Fragments arranged by size:
3257 2229 2086 1701 1605 814 552 513
375
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13132
NgoMIV G'CCGG_C
Cuts at: 2879 2930 4355 4389 4722 5429 8585 10186 2879
Size: 51 1425 34 333 707 3156 1601 5825
Fragments arranged by size:
5825 3156 1601 1425 707 333 51 34
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13132
NotI GC'GGCC_GC
Cuts at: 10579 10579
Size: 13132
NsiI A_TGCA'T
Cuts at: 5981 10263 5981
Size: 4282 8850
NspI r_CATG'y
Cuts at: 1132 1229 2865 3465 5586 5650 5712 6987 7492
Size: 97 1636 600 2121 64 62 1275 505
Cuts at: 7492 8427 8725 10261 10490 12035 1132
Size: 935 298 1536 229 1545 2229
Fragments arranged by size:
2229 2121 1636 1545 1536 1275 935 600
505 298 229 97 64 62
PasI CC'CwG_GG
Cuts at: 1348 5303 5906 6571 6877 7970 9667 10356 1348
Size: 3955 603 665 306 1093 1697 689 4124
Fragments arranged by size:
4124 3955 1697 1093 689 665 603 306
PciI A'CATG_T
Cuts at: 5708 6983 7488 12031 5708
Size: 1275 505 4543 6809
Fragments arranged by size:
6809 4543 1275 505
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 3799 6217 9703 9835 1108
Size: 1638 1053 2418 3486 132 4405
Fragments arranged by size:
4405 3486 2418 1638 1053 132
PfoI T'CCnGG_A
Cuts at: 2128 4452 6483 10173 2128
Size: 2324 2031 3690 5087
Fragments arranged by size:
5087 3690 2324 2031
PmeI GTTT'AAAC
Cuts at: 10436 10436
Size: 13132
PmlI CAC'GTG
Cuts at: 3850 3850
Size: 13132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11201 11233 12714 12746 11201
Size: 32 1481 32 11587
Fragments arranged by size:
11587 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2126 2528 2558 3119 5236 7581 9238 9330 10352
Size: 402 30 561 2117 2345 1657 92 1022
Cuts at: 10352 2126
Size: 4906
Fragments arranged by size:
4906 2345 2117 1657 1022 561 402 92
30
PshAI GACnn'nnGTC
Cuts at: 1613 4595 5097 1613
Size: 2982 502 9648
Fragments arranged by size:
9648 2982 502
PsiI TTA'TAA
Cuts at: 10702 10702
Size: 13132
PspOMI G'GGCC_C
Cuts at: 2035 2492 3184 8897 9002 9605 9693 2035
Size: 457 692 5713 105 603 88 5474
Fragments arranged by size:
5713 5474 692 603 457 105 88
PspXI vC'TCGA_Gb
Cuts at: 7847 7847
Size: 13132
PstI C_TGCA'G
Cuts at: 830 2125 5012 6413 6524 6907 7525 8129 8922
Size: 1295 2887 1401 111 383 618 604 793
Cuts at: 8922 9659 10290 10425 10484 830
Size: 737 631 135 59 3478
Fragments arranged by size:
3478 2887 1401 1295 793 737 631 618
604 383 135 111 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13132
PvuII CAG'CTG
Cuts at: 2337 2602 2790 2984 4248 5544 5803 5919 6414
Size: 265 188 194 1264 1296 259 116 495
Cuts at: 6414 7724 7769 9294 9660 9876 10291 10420 2337
Size: 1310 45 1525 366 216 415 129 5049
Fragments arranged by size:
5049 1525 1310 1296 1264 495 415 366
265 259 216 194 188 129 116 45
SacI G_AGCT'C
Cuts at: 3570 7764 8205 9018 10182 3570
Size: 4194 441 813 1164 6520
Fragments arranged by size:
6520 4194 1164 813 441
SacII CC_GC'GG
Cuts at: 2464 2707 4907 8820 10234 2464
Size: 243 2200 3913 1414 5362
Fragments arranged by size:
5362 3913 2200 1414 243
SalI G'TCGA_C
Cuts at: 1936 4304 10474 1936
Size: 2368 6170 4594
Fragments arranged by size:
6170 4594 2368
SanDI GG'GwC_CC
Cuts at: 2558 3119 5236 2558
Size: 561 2117 10454
Fragments arranged by size:
10454 2117 561
SbfI CC_TGCA'GG
Cuts at: 10484 10484
Size: 13132
ScaI AGT'ACT
Cuts at: 11380 13092 11380
Size: 1712 11420
SexAI A'CCwGG_T
Cuts at: 10089 10089
Size: 13132
SfcI C'TryA_G
Cuts at: 826 1045 2121 2260 4243 5008 5920 6409 6520
Size: 219 1076 139 1983 765 912 489 111
Cuts at: 6520 6903 7450 7521 8125 8347 8918 9043 9655
Size: 383 547 71 604 222 571 125 612
Cuts at: 9655 9865 9871 10286 10421 10480 11615 12296 12487
Size: 210 6 415 135 59 1135 681 191
Cuts at: 12487 826
Size: 1471
Fragments arranged by size:
1983 1471 1135 1076 912 765 681 612
604 571 547 489 415 383 222 219
210 191 139 135 125 111 71 59
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2066 2577 3075 2066
Size: 511 498 12123
Fragments arranged by size:
12123 511 498
SfoI GGC'GCC
Cuts at: 2884 3696 9403 2884
Size: 812 5707 6613
Fragments arranged by size:
6613 5707 812
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13132
SgrAI Cr'CCGG_yG
Cuts at: 1700 5994 1700
Size: 4294 8838
SmaI CCC'GGG
Cuts at: 1872 3432 4574 4635 6645 7128 8666 9522 10459
Size: 1560 1142 61 2010 483 1538 856 937
Cuts at: 10459 1872
Size: 4545
Fragments arranged by size:
4545 2010 1560 1538 1142 937 856 483
61
SmlI C'TyrA_G
Cuts at: 820 1017 1943 7847 9915 10402 11236 12137 12399
Size: 197 926 5904 2068 487 834 901 262
Cuts at: 12399 820
Size: 1553
Fragments arranged by size:
5904 2068 1553 926 901 834 487 262
197
SnaBI TAC'GTA
Cuts at: 493 10923 493
Size: 10430 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13132
SphI G_CATG'C
Cuts at: 1132 3465 8725 10261 10490 1132
Size: 2333 5260 1536 229 3774
Fragments arranged by size:
5260 3774 2333 1536 229
SspI AAT'ATT
Cuts at: 5 52 3202 11056 5
Size: 47 3150 7854 2081
Fragments arranged by size:
7854 3150 2081 47
StuI AGG'CCT
Cuts at: 1517 2772 4862 7052 7073 7517 8137 9449 10400
Size: 1255 2090 2190 21 444 620 1312 951
Cuts at: 10400 1517
Size: 4249
Fragments arranged by size:
4249 2190 2090 1312 1255 951 620 444
21
StyI C'CwwG_G
Cuts at: 513 1065 2092 2112 2622 3151 4428 5322 5380
Size: 552 1027 20 510 529 1277 894 58
Cuts at: 5380 5629 5755 7456 9061 9151 9547 9574 10131
Size: 249 126 1701 1605 90 396 27 557
Cuts at: 10131 10388 10571 513
Size: 257 183 3074
Fragments arranged by size:
3074 1701 1605 1277 1027 894 557 552
529 510 396 257 249 183 126 90
58 27 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3338 3860 5487 5929 6330 6513 9449
Size: 1255 1758 522 1627 442 401 183 2936
Cuts at: 9449 9692 11159 11176 11329 11514 11959 325
Size: 243 1467 17 153 185 445 1498
Fragments arranged by size:
2936 1758 1627 1498 1467 1255 522 445
442 401 243 185 183 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6627 7673 11378
Size: 274 80 33 51 1443 4650 1046 3705
Cuts at: 11378 13090 96
Size: 1712 138
Fragments arranged by size:
4650 3705 1712 1443 1046 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2230 2262 4579 4611 5981 6013 6229 6261 7768
Size: 32 2317 32 1370 32 216 32 1507
Cuts at: 7768 7800 9935 9967 13092 13124 2230
Size: 32 2135 32 3125 32 2238
Fragments arranged by size:
3125 2317 2238 2135 1507 1370 216 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3392 6740 1316
Size: 2076 3348 7708
Fragments arranged by size:
7708 3348 2076
XbaI T'CTAG_A
Cuts at: 4417 10201 10468 4417
Size: 5784 267 7081
Fragments arranged by size:
7081 5784 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4232 5300 6923 7109 4232
Size: 1068 1623 186 10255
Fragments arranged by size:
10255 1623 1068 186
XhoI C'TCGA_G
Cuts at: 1943 7847 1943
Size: 5904 7228
XmaI C'CCGG_G
Cuts at: 1870 3430 4572 4633 6643 7126 8664 9520 10457
Size: 1560 1142 61 2010 483 1538 856 937
Cuts at: 10457 1870
Size: 4545
Fragments arranged by size:
4545 2010 1560 1538 1142 937 856 483
61
XmnI GAAnn'nnTTC
Cuts at: 6479 11261 6479
Size: 4782 8350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4816 10940 276
Size: 53 83 186 936 3282 6124 2468
Fragments arranged by size:
6124 3282 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AvrII BsiWI BstZ17I FspAI NruI PacI PsrI RsrII
SapI SrfI SwaI