Restriction map for
pFN21AA1763
(Circular) (Six-base) MAPSORT of: pFN21AA1763.seq Check: 8299 from: 1 to: 8629
pFN21AA1763 8629 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3326 5471 3326
Size: 2145 6484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6439 278
Size: 53 83 186 936 4903 2468
Fragments arranged by size:
4903 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5850 5972 1937
Size: 3913 122 4594
Fragments arranged by size:
4594 3913 122
Acc65I G'GTAC_C
Cuts at: 5950 5950
Size: 8629
AclI AA'CG_TT
Cuts at: 1563 6756 7129 1563
Size: 5193 373 3063
Fragments arranged by size:
5193 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 3224 3604 3614 3798 3986 5561 6692
Size: 487 1105 380 10 184 188 1575 1131
Cuts at: 6692 8076 8344 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1575 1384 1131 1105 487 380 268
188 184 10
AflII C'TTAA_G
Cuts at: 820 1017 3287 820
Size: 197 2270 6162
Fragments arranged by size:
6162 2270 197
AflIII A'CryG_T
Cuts at: 1203 5590 7528 8505 1203
Size: 4387 1938 977 1327
Fragments arranged by size:
4387 1938 1327 977
AgeI A'CCGG_T
Cuts at: 7460 7460
Size: 8629
AhdI GACnn_n'nnGTC
Cuts at: 7358 7358
Size: 8629
AleI CACnn'nnGTG
Cuts at: 1166 2282 3399 1166
Size: 1116 1117 6396
Fragments arranged by size:
6396 1117 1116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8629
AlwNI CAG_nnn'CTG
Cuts at: 2391 2957 5573 6372 7944 2391
Size: 566 2616 799 1572 3076
Fragments arranged by size:
3076 2616 1572 799 566
ApaLI G'TGCA_C
Cuts at: 6686 7842 6686
Size: 1156 7473
ApoI r'AATT_y
Cuts at: 1493 2083 2636 2903 2986 4381 5132 5405 5938
Size: 590 553 267 83 1395 751 273 533
Cuts at: 5938 6164 6316 8425 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1395 751 590 553 533 273
267 226 152 83
AseI AT'TA_AT
Cuts at: 160 1032 2739 7183 160
Size: 872 1707 4444 1606
Fragments arranged by size:
4444 1707 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8629
AvaI C'yCGr_G
Cuts at: 1870 1943 5954 1870
Size: 73 4011 4545
Fragments arranged by size:
4545 4011 73
BamHI G'GATC_C
Cuts at: 3509 5959 6326 3509
Size: 2450 367 5812
Fragments arranged by size:
5812 2450 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3470 4632 5950 7405 618
Size: 325 217 627 1683 1162 1318 1455 1842
Fragments arranged by size:
1842 1683 1455 1318 1162 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2154 2612 3134 4849 5183 5697 5948 1391
Size: 763 458 522 1715 334 514 251 4072
Fragments arranged by size:
4072 1715 763 522 514 458 334 251
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7658
BbvCI CC'TCA_GC
Cuts at: 5859 5859
Size: 8629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6820 6854 1903
Size: 34 4883 34 3678
Fragments arranged by size:
4883 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 6521 7731 3269
Size: 3252 1210 4167
Fragments arranged by size:
4167 3252 1210
BclI T'GATC_A
Cuts at: 1548 2593 2809 5127 5208 1548
Size: 1045 216 2318 81 4969
Fragments arranged by size:
4969 2318 1045 216 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7240 136
Size: 107 122 71 631 605 5568 1525
Fragments arranged by size:
5568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3583 8624 3583
Size: 5041 3588
BlpI GC'TnA_GC
Cuts at: 5114 5522 6046 5114
Size: 408 524 7697
Fragments arranged by size:
7697 524 408
Bme1580I G_kGCm'C
Cuts at: 1790 6690 7846 1790
Size: 4900 1156 2573
Fragments arranged by size:
4900 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 3315 3694
Size: 416 53 83 399 24 909 989 379
Cuts at: 3694 4147 4352 6360 7318 442
Size: 453 205 2008 958 1753
Fragments arranged by size:
2008 1753 989 958 909 453 416 399
379 205 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8629
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2045 2741 4555 2045
Size: 696 1814 6119
Fragments arranged by size:
6119 1814 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 2426 3118 3186 3727 4842 5059 7289
Size: 901 286 692 68 541 1115 217 2230
Cuts at: 7289 1239
Size: 2579
Fragments arranged by size:
2579 2230 1115 901 692 541 286 217
68
Bpu10I CC'TnA_GC
Cuts at: 2606 3450 5859 2606
Size: 844 2409 5376
Fragments arranged by size:
5376 2409 844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2277 3418 6754 7619 7917 2015
Size: 262 1141 3336 865 298 2727
Fragments arranged by size:
3336 2727 1141 865 298 262
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2220 4809 7292 882
Size: 617 721 2589 2483 2219
Fragments arranged by size:
2589 2483 2219 721 617
BsaAI yAC'GTr
Cuts at: 493 1204 5530 5593 6420 493
Size: 711 4326 63 827 2702
Fragments arranged by size:
4326 2702 827 711 63
BsaBI GATnn'nnATC
Cuts at: 5759 8623 5759
Size: 2864 5765
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6436 6818 7453 275
Size: 53 83 186 936 4903 382 635 1451
Fragments arranged by size:
4903 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4230 7062 7460 7734 7881 8292 4230
Size: 2832 398 274 147 411 4567
Fragments arranged by size:
4567 2832 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5063 5093 1178
Size: 30 165 30 3660 30 4714
Fragments arranged by size:
4714 3660 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3185 3188 3254 3426 3762 1186
Size: 1999 3 66 172 336 6053
Fragments arranged by size:
6053 1999 336 172 66 3
BseYI C'CCAG_C
Cuts at: 2078 3442 3965 5558 5616 7832 8328 2078
Size: 1364 523 1593 58 2216 496 2379
Fragments arranged by size:
2379 2216 1593 1364 523 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4041 4619 4886 4945 5433 5457 5537
Size: 573 2305 578 267 59 488 24 80
Cuts at: 5537 1163
Size: 4255
Fragments arranged by size:
4255 2305 578 573 488 267 80 59
24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6079 6840 7868 1540
Size: 275 186 4078 761 1028 2301
Fragments arranged by size:
4078 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 2832 3134 3713 4029 5183 6690 6775 7846
Size: 678 302 579 316 1154 1507 85 1071
Cuts at: 7846 2154
Size: 2937
Fragments arranged by size:
2937 1507 1154 1071 678 579 316 302
85
BsmI GAATG_Cn'
Cuts at: 2517 2821 3364 4321 4797 6140 6233 2517
Size: 304 543 957 476 1343 93 4913
Fragments arranged by size:
4913 1343 957 543 476 304 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2612 2832 3134 3713 4029 4849
Size: 399 364 458 220 302 579 316 820
Cuts at: 4849 5183 5697 5948 6690 6775 7846 1391
Size: 334 514 251 742 85 1071 2174
Fragments arranged by size:
2174 1071 820 742 579 514 458 399
364 334 316 302 251 220 85
BspEI T'CCGG_A
Cuts at: 8292 8292
Size: 8629
BspHI T'CATG_A
Cuts at: 2913 6518 8248 2913
Size: 3605 1730 3294
Fragments arranged by size:
3605 3294 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 5471 5984 844
Size: 2482 2145 513 3489
Fragments arranged by size:
3489 2482 2145 513
BsrBI CCG'CTC
Cuts at: 1394 1823 6516 1394
Size: 429 4693 3507
Fragments arranged by size:
4693 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3066 4885 4921 5211 7124 7298 65
Size: 1183 1818 1819 36 290 1913 174 1396
Fragments arranged by size:
1913 1819 1818 1396 1183 290 174 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 7273 7460 1700
Size: 110 5463 187 2869
Fragments arranged by size:
5463 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8629
BssSI C'ACGA_G
Cuts at: 6689 7701 6689
Size: 1012 7617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2827 794
Size: 2033 6596
BstBI TT'CG_AA
Cuts at: 3199 7433 3199
Size: 4234 4395
BstEII G'GTnAC_C
Cuts at: 1184 2275 2320 1184
Size: 1091 45 7493
Fragments arranged by size:
7493 1091 45
BstXI CCAn_nnnn'nTGG
Cuts at: 5611 5611
Size: 8629
BstYI r'GATC_y
Cuts at: 1972 2009 3509 3583 5018 5959 6326 6711 6728
Size: 37 1500 74 1435 941 367 385 17
Cuts at: 6728 8180 8266 8278 8624 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1500 1452 1435 941 385 367 346
86 74 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2124 2124
Size: 8629
BtgI C'CryG_G
Cuts at: 513 1065 2004 4432 513
Size: 552 939 2428 4710
Fragments arranged by size:
4710 2428 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7890
Fragments arranged by size:
7890 683 56
BtsI GCAGTG_nn'
Cuts at: 2702 3149 3365 5520 5775 6145 6939 6959 2702
Size: 447 216 2155 255 370 794 20 4372
Fragments arranged by size:
4372 2155 794 447 370 255 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8594
DraI TTT'AAA
Cuts at: 5290 5933 5290
Size: 643 7986
DraIII CAC_nnn'GTG
Cuts at: 3533 4639 3533
Size: 1106 7523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7636 809
Size: 507 6320 1802
Fragments arranged by size:
6320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 3042 3415
Size: 54 1425 50 275 18 456 756 373
Cuts at: 3415 3984 4767 6076 6965 8594 8
Size: 569 783 1309 889 1629 43
Fragments arranged by size:
1629 1425 1309 889 783 756 569 456
373 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6076 1537
Size: 275 4264 4090
Fragments arranged by size:
4264 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2188 2710 4757 6559 1353
Size: 835 522 2047 1802 3423
Fragments arranged by size:
3423 2047 1802 835 522
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7216 7590 7736 387
Size: 1064 138 5627 374 146 1280
Fragments arranged by size:
5627 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 3132 5181 2152
Size: 980 2049 5600
Fragments arranged by size:
5600 2049 980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2140 2426 3118 3186 3224 3604
Size: 393 487 21 286 692 68 38 380
Cuts at: 3604 3614 3727 3798 3986 4842 5059 5561 6692
Size: 10 113 71 188 856 217 502 1131
Cuts at: 6692 7289 8076 8344 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1131 856 787 692 597 502 487
393 380 286 268 217 188 113 71
68 38 21 10
EcoO109I rG'GnC_Cy
Cuts at: 3446 3606 4346 3446
Size: 160 740 7729
Fragments arranged by size:
7729 740 160
EcoRI G'AATT_C
Cuts at: 2083 2903 4381 5405 5938 6316 2083
Size: 820 1478 1024 533 378 4396
Fragments arranged by size:
4396 1478 1024 820 533 378
EcoRV GAT'ATC
Cuts at: 3031 3031
Size: 8629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3412 4295 5216 3412
Size: 883 921 6825
Fragments arranged by size:
6825 921 883
FspI TGC'GCA
Cuts at: 5487 7135 5487
Size: 1648 6981
HaeII r_GCGC'y
Cuts at: 1678 7776 1678
Size: 6098 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 2682 2714 3015
Size: 32 135 32 1013 32 65 32 301
Cuts at: 3015 3047 5112 5144 5621 5653 7270 7302 7344
Size: 32 2065 32 477 32 1617 32 42
Cuts at: 7344 7376 1373
Size: 32 2626
Fragments arranged by size:
2626 2065 1617 1013 477 301 135 65
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 5278 5973 6219 669
Size: 988 281 919 2421 695 246 3079
Fragments arranged by size:
3079 2421 988 919 695 281 246
HindIII A'AGCT_T
Cuts at: 748 3502 5161 748
Size: 2754 1659 4216
Fragments arranged by size:
4216 2754 1659
HpaI GTT'AAC
Cuts at: 5278 6219 5278
Size: 941 7688
KpnI G_GTAC'C
Cuts at: 5954 5954
Size: 8629
MfeI C'AATT_G
Cuts at: 4216 6228 4216
Size: 2012 6617
MluI A'CGCG_T
Cuts at: 8505 8505
Size: 8629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 4568 7743 7927 1305
Size: 1032 2231 3175 184 2007
Fragments arranged by size:
3175 2231 2007 1032 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 3044 3986 10
Size: 54 1425 343 456 756 942 4653
Fragments arranged by size:
4653 1425 942 756 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 2889 3071 3399 5454 6587 6946
Size: 648 1116 607 182 328 2055 1133 359
Cuts at: 6946 7105 518
Size: 159 2042
Fragments arranged by size:
2055 2042 1133 1116 648 607 359 328
182 159
MspA1I CmG'CkG
Cuts at: 1606 2282 3244 3570 4687 4715 5607 6045 6722
Size: 676 962 326 1117 28 892 438 677
Cuts at: 6722 7870 8115 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1117 962 892 677 676 438
326 245 28
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7138
Fragments arranged by size:
7138 939 552
NdeI CA'TA_TG
Cuts at: 387 3961 387
Size: 3574 5055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8629
NotI GC'GGCC_GC
Cuts at: 6076 6076
Size: 8629
NspI r_CATG'y
Cuts at: 1132 1229 4727 5987 7532 1132
Size: 97 3498 1260 1545 2229
Fragments arranged by size:
3498 2229 1545 1260 97
PasI CC'CwG_GG
Cuts at: 1348 2791 1348
Size: 1443 7186
PciI A'CATG_T
Cuts at: 7528 7528
Size: 8629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 2957 3533 3965 1108
Size: 1734 115 576 432 5772
Fragments arranged by size:
5772 1734 576 432 115
PfoI T'CCnGG_A
Cuts at: 2170 3134 3704 2170
Size: 964 570 7095
Fragments arranged by size:
7095 964 570
PmeI GTTT'AAAC
Cuts at: 5933 5933
Size: 8629
PmlI CAC'GTG
Cuts at: 5530 5593 5530
Size: 63 8566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6698 6730 8211 8243 6698
Size: 32 1481 32 7084
Fragments arranged by size:
7084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3606 3606
Size: 8629
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8629
PsiI TTA'TAA
Cuts at: 3018 4269 4338 6199 3018
Size: 1251 69 1861 5448
Fragments arranged by size:
5448 1861 1251 69
PstI C_TGCA'G
Cuts at: 830 4812 5515 5728 5981 830
Size: 3982 703 213 253 3478
Fragments arranged by size:
3982 3478 703 253 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8629
PvuII CAG'CTG
Cuts at: 3244 4687 5607 3244
Size: 1443 920 6266
Fragments arranged by size:
6266 1443 920
SacI G_AGCT'C
Cuts at: 2154 3134 5183 2154
Size: 980 2049 5600
Fragments arranged by size:
5600 2049 980
SalI G'TCGA_C
Cuts at: 1936 5971 1936
Size: 4035 4594
SanDI GG'GwC_CC
Cuts at: 3606 3606
Size: 8629
SapI GCTCTTCn'nnn_
Cuts at: 2710 4757 2710
Size: 2047 6582
SbfI CC_TGCA'GG
Cuts at: 5981 5981
Size: 8629
ScaI AGT'ACT
Cuts at: 5921 6877 8589 5921
Size: 956 1712 5961
Fragments arranged by size:
5961 1712 956
SfcI C'TryA_G
Cuts at: 826 1045 2434 2584 4808 5511 5724 5977 7112
Size: 219 1389 150 2224 703 213 253 1135
Cuts at: 7112 7793 7984 826
Size: 681 191 1471
Fragments arranged by size:
2224 1471 1389 1135 703 681 253 219
213 191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8629
SmaI CCC'GGG
Cuts at: 1872 5956 1872
Size: 4084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 2292 3287 3397 6733 7634
Size: 197 926 87 262 995 110 3336 901
Cuts at: 7634 7896 820
Size: 262 1553
Fragments arranged by size:
3336 1553 995 926 901 262 262 197
110 87
SnaBI TAC'GTA
Cuts at: 493 6420 493
Size: 5927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8629
SphI G_CATG'C
Cuts at: 1132 5987 1132
Size: 4855 3774
SspI AAT'ATT
Cuts at: 5 52 6553 5
Size: 47 6501 2081
Fragments arranged by size:
6501 2081 47
StuI AGG'CCT
Cuts at: 1517 4667 1517
Size: 3150 5479
StyI C'CwwG_G
Cuts at: 513 1065 2004 2257 2496 3610 6068 513
Size: 552 939 253 239 1114 2458 3074
Fragments arranged by size:
3074 2458 1114 939 552 253 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 3899 4621 4625 4748 4791 5580
Size: 1255 728 1591 722 4 123 43 789
Cuts at: 5580 5612 6656 6673 6826 7011 7456 325
Size: 32 1044 17 153 185 445 1498
Fragments arranged by size:
1591 1498 1255 1044 789 728 722 445
185 153 123 43 32 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 4169 5919
Size: 274 80 33 51 1443 453 1739 1750
Cuts at: 5919 6875 8587 96
Size: 956 1712 138
Fragments arranged by size:
1750 1739 1712 1443 956 453 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2952 2984 3607 3639 8589 8621 2952
Size: 32 623 32 4950 32 2960
Fragments arranged by size:
4950 2960 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5573 5599 1316
Size: 4257 26 4346
Fragments arranged by size:
4346 4257 26
XbaI T'CTAG_A
Cuts at: 5965 5965
Size: 8629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 2773
Size: 8629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8629
XmaI C'CCGG_G
Cuts at: 1870 5954 1870
Size: 4084 4545
XmnI GAAnn'nnTTC
Cuts at: 2572 3811 3861 4278 6758 2572
Size: 1239 50 417 2480 4443
Fragments arranged by size:
4443 2480 1239 417 50
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6437 276
Size: 53 83 186 936 4903 2468
Fragments arranged by size:
4903 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI