Restriction map for
pFN21AA1761
(Circular) (Six-base) MAPSORT of: pFN21AA1761.seq Check: 2672 from: 1 to: 7216
pFN21AA1761 7216 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3956 3956
Size: 7216
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3648 5026 278
Size: 53 83 186 936 2112 1378 2468
Fragments arranged by size:
2468 2112 1378 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3786 4559 1937
Size: 1849 773 4594
Fragments arranged by size:
4594 1849 773
Acc65I G'GTAC_C
Cuts at: 4537 4537
Size: 7216
AclI AA'CG_TT
Cuts at: 1563 5343 5716 1563
Size: 3780 373 3063
Fragments arranged by size:
3780 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2731 3009 3599 4166 5279 6663 6931 1632
Size: 1099 278 590 567 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1113 1099 590 567 278 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7019
AflIII A'CryG_T
Cuts at: 1203 3429 6115 7092 1203
Size: 2226 2686 977 1327
Fragments arranged by size:
2686 2226 1327 977
AgeI A'CCGG_T
Cuts at: 6047 6047
Size: 7216
AhdI GACnn_n'nnGTC
Cuts at: 5945 5945
Size: 7216
AleI CACnn'nnGTG
Cuts at: 1166 3371 3671 1166
Size: 2205 300 4711
Fragments arranged by size:
4711 2205 300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3534 1184
Size: 2350 4866
AlwNI CAG_nnn'CTG
Cuts at: 3020 3814 4959 6531 3020
Size: 794 1145 1572 3705
Fragments arranged by size:
3705 1572 1145 794
ApaI G_GGCC'C
Cuts at: 2033 4108 4363 2033
Size: 2075 255 4886
Fragments arranged by size:
4886 2075 255
ApaLI G'TGCA_C
Cuts at: 2890 5273 6429 2890
Size: 2383 1156 3677
Fragments arranged by size:
3677 2383 1156
ApoI r'AATT_y
Cuts at: 1493 2282 2819 3332 4525 4751 4903 7012 1493
Size: 789 537 513 1193 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 789 537 513 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5770 160
Size: 872 4738 1606
Fragments arranged by size:
4738 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2439 3138 4541 1870
Size: 73 159 337 699 1403 4545
Fragments arranged by size:
4545 1403 699 337 159 73
BamHI G'GATC_C
Cuts at: 2322 3115 4546 4913 2322
Size: 793 1431 367 4625
Fragments arranged by size:
4625 1431 793 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 4537 5992 618
Size: 325 217 627 320 2430 1455 1842
Fragments arranged by size:
2430 1842 1455 627 325 320 217
BanII G_rGCy'C
Cuts at: 1391 2033 2250 2765 3270 3280 4108 4363 4535
Size: 642 217 515 505 10 828 255 172
Cuts at: 4535 1391
Size: 4072
Fragments arranged by size:
4072 828 642 515 505 255 217 172
10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2885 4211 4471 928
Size: 971 986 1326 260 3673
Fragments arranged by size:
3673 1326 986 971 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2232 2266 5407 5441 1903
Size: 34 295 34 3141 34 3678
Fragments arranged by size:
3678 3141 295 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2350 5108 6318 2350
Size: 2758 1210 3248
Fragments arranged by size:
3248 2758 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7216
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5827 136
Size: 107 122 71 631 605 4155 1525
Fragments arranged by size:
4155 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7211 7211
Size: 7216
BlpI GC'TnA_GC
Cuts at: 4633 4633
Size: 7216
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2110 2546 2894 4108 4315 4363 5277
Size: 243 77 436 348 1214 207 48 914
Cuts at: 5277 6433 1790
Size: 1156 2573
Fragments arranged by size:
2573 1214 1156 914 436 348 243 207
77 48
BmgBI CAC'GTC
Cuts at: 1318 3432 1318
Size: 2114 5102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4264 4947 5905
Size: 416 53 83 399 24 2847 683 958
Cuts at: 5905 442
Size: 1753
Fragments arranged by size:
2847 1753 958 683 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2500 2779 4498 2465
Size: 35 279 1719 5183
Fragments arranged by size:
5183 1719 279 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2555 3197 4009 5876 1239
Size: 989 327 642 812 1867 2579
Fragments arranged by size:
2579 1867 989 812 642 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2445 3335 3746 4139 5341 6206 6504 2414
Size: 31 890 411 393 1202 865 298 3126
Fragments arranged by size:
3126 1202 890 865 411 393 298 31
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2681 5879 882
Size: 617 1182 3198 2219
Fragments arranged by size:
3198 2219 1182 617
BsaAI yAC'GTr
Cuts at: 493 1204 5007 493
Size: 711 3803 2702
Fragments arranged by size:
3803 2702 711
BsaBI GATnn'nnATC
Cuts at: 7210 7210
Size: 7216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3645 5023 5405 6040
Size: 53 83 186 936 2112 1378 382 635
Cuts at: 6040 275
Size: 1451
Fragments arranged by size:
2112 1451 1378 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2319 2370 5649 6047 6321 6468 6879 2319
Size: 51 3279 398 274 147 411 2656
Fragments arranged by size:
3279 2656 411 398 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 3461 3491 1178
Size: 30 165 30 798 30 1230 30 4903
Fragments arranged by size:
4903 1230 798 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 2519 2924 3167 3476 3671 4319 1186
Size: 1138 195 405 243 309 195 648 4083
Fragments arranged by size:
4083 1138 648 405 309 243 195 195
BseYI C'CCAG_C
Cuts at: 2058 2758 3548 3691 3807 6419 6915 2058
Size: 700 790 143 116 2612 496 2359
Fragments arranged by size:
2612 2359 790 700 496 143 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2500 2519 1163
Size: 573 764 19 5860
Fragments arranged by size:
5860 764 573 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4666 5427 6455 1540
Size: 275 186 2665 761 1028 2301
Fragments arranged by size:
2665 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 2894 3069 5277 5362 6433 2250
Size: 644 175 2208 85 1071 3033
Fragments arranged by size:
3033 2208 1071 644 175 85
BsmI GAATG_Cn'
Cuts at: 3831 4051 4727 4820 3831
Size: 220 676 93 6227
Fragments arranged by size:
6227 676 220 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3179 3179
Size: 7216
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2110 2250 2546 2765 2894 3069
Size: 399 243 77 140 296 219 129 175
Cuts at: 3069 3270 3280 4108 4315 4363 4535 5277 5362
Size: 201 10 828 207 48 172 742 85
Cuts at: 5362 6433 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 828 742 399 296 243 219
207 201 175 172 140 129 85 77
48 10
BspEI T'CCGG_A
Cuts at: 2319 6879 2319
Size: 4560 2656
BspHI T'CATG_A
Cuts at: 5105 6835 5105
Size: 1730 5486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3956 4504 4571 844
Size: 3112 548 67 3489
Fragments arranged by size:
3489 3112 548 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3112 3477 5103 1394
Size: 429 1289 365 1626 3507
Fragments arranged by size:
3507 1626 1289 429 365
BsrDI GCAATG_nn'
Cuts at: 65 1248 4273 5711 5885 65
Size: 1183 3025 1438 174 1396
Fragments arranged by size:
3025 1438 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 2080 5860 6047 1700
Size: 110 201 69 3780 187 2869
Fragments arranged by size:
3780 2869 201 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 2772 96
Size: 2676 4540
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7216
BssSI C'ACGA_G
Cuts at: 5276 6288 5276
Size: 1012 6204
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3934 794
Size: 3140 4076
BstBI TT'CG_AA
Cuts at: 6020 6020
Size: 7216
BstEII G'GTnAC_C
Cuts at: 1184 2812 1184
Size: 1628 5588
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 4174 2632
Size: 1542 5674
BstYI r'GATC_y
Cuts at: 1972 2322 3115 3582 4023 4546 4913 5298 5315
Size: 350 793 467 441 523 367 385 17
Cuts at: 5315 6767 6853 6865 7211 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 793 523 467 441 385 367
350 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2651 2967 3654 4434 2651
Size: 316 687 780 5433
Fragments arranged by size:
5433 780 687 316
BtgI C'CryG_G
Cuts at: 513 1065 2004 2111 2121 3272 513
Size: 552 939 107 10 1151 4457
Fragments arranged by size:
4457 1151 939 552 107 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6477
Fragments arranged by size:
6477 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 2611 2833 3361 3879 3916 4190 4732 5526
Size: 202 222 528 518 37 274 542 794
Cuts at: 5526 5546 2409
Size: 20 4079
Fragments arranged by size:
4079 794 542 528 518 274 222 202
37 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7181
DraI TTT'AAA
Cuts at: 4520 4520
Size: 7216
DraIII CAC_nnn'GTG
Cuts at: 3817 3922 3817
Size: 105 7111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2654 6223 809
Size: 507 1338 3569 1802
Fragments arranged by size:
3569 1802 1338 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4663 5552 7181
Size: 54 1425 50 275 18 2833 889 1629
Cuts at: 7181 8
Size: 43
Fragments arranged by size:
2833 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4663 1537
Size: 275 2851 4090
Fragments arranged by size:
4090 2851 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2417 3138 3144 4497 5146 1353
Size: 1064 721 6 1353 649 3423
Fragments arranged by size:
3423 1353 1064 721 649 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2064 3965 5803 6177 6323 387
Size: 1064 138 475 1901 1838 374 146 1280
Fragments arranged by size:
1901 1838 1280 1064 475 374 146 138
EcoICRI GAG'CTC
Cuts at: 2248 2248
Size: 7216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2555 2731 3009 3197 3599 4009
Size: 393 596 327 176 278 188 402 410
Cuts at: 4009 4166 5279 5876 6663 6931 1239
Size: 157 1113 597 787 268 1524
Fragments arranged by size:
1524 1113 787 597 596 410 402 393
327 278 268 188 176 157
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 3486 4433 2534
Size: 952 947 5317
Fragments arranged by size:
5317 952 947
EcoO109I rG'GnC_Cy
Cuts at: 2656 4105 2656
Size: 1449 5767
EcoRI G'AATT_C
Cuts at: 2282 4525 4903 2282
Size: 2243 378 4595
Fragments arranged by size:
4595 2243 378
FspI TGC'GCA
Cuts at: 5722 5722
Size: 7216
HaeII r_GCGC'y
Cuts at: 1678 4351 6363 1678
Size: 2673 2012 2531
Fragments arranged by size:
2673 2531 2012
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2486 2518 2680 2712 5857
Size: 32 135 32 914 32 162 32 3145
Cuts at: 5857 5889 5931 5963 1373
Size: 32 42 32 2626
Fragments arranged by size:
3145 2626 914 162 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4560 4806 669
Size: 988 281 2622 246 3079
Fragments arranged by size:
3079 2622 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7216
HpaI GTT'AAC
Cuts at: 4806 4806
Size: 7216
KpnI G_GTAC'C
Cuts at: 4541 4541
Size: 7216
MfeI C'AATT_G
Cuts at: 4815 4815
Size: 7216
MluI A'CGCG_T
Cuts at: 7092 7092
Size: 7216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6330 6514 1305
Size: 5025 184 2007
Fragments arranged by size:
5025 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5394
Fragments arranged by size:
5394 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3305 3371 3671 5174 5533 5692
Size: 648 1815 324 66 300 1503 359 159
Cuts at: 5692 518
Size: 2042
Fragments arranged by size:
2042 1815 1503 648 359 324 300 159
66
MspA1I CmG'CkG
Cuts at: 1606 2113 2123 2770 4632 5309 6457 6702 1606
Size: 507 10 647 1862 677 1148 245 2120
Fragments arranged by size:
2120 1862 1148 677 647 507 245 10
NaeI GCC'GGC
Cuts at: 2013 2082 2013
Size: 69 7147
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5725
Fragments arranged by size:
5725 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7216
NgoMIV G'CCGG_C
Cuts at: 2011 2080 2011
Size: 69 7147
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7216
NotI GC'GGCC_GC
Cuts at: 4663 4663
Size: 7216
NspI r_CATG'y
Cuts at: 1132 1229 4574 6119 1132
Size: 97 3345 1545 2229
Fragments arranged by size:
3345 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7216
PciI A'CATG_T
Cuts at: 6115 6115
Size: 7216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3814 4423 4497 1108
Size: 2706 609 74 3827
Fragments arranged by size:
3827 2706 609 74
PfoI T'CCnGG_A
Cuts at: 2391 2504 2532 3903 2391
Size: 113 28 1371 5704
Fragments arranged by size:
5704 1371 113 28
PmeI GTTT'AAAC
Cuts at: 4520 4520
Size: 7216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5285 5317 6798 6830 5285
Size: 32 1481 32 5671
Fragments arranged by size:
5671 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2656 2656
Size: 7216
PshAI GACnn'nnGTC
Cuts at: 1613 3171 1613
Size: 1558 5658
PsiI TTA'TAA
Cuts at: 4786 4786
Size: 7216
PspOMI G'GGCC_C
Cuts at: 2029 4104 4359 2029
Size: 2075 255 4886
Fragments arranged by size:
4886 2075 255
PspXI vC'TCGA_Gb
Cuts at: 2439 2439
Size: 7216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 7184
PstI C_TGCA'G
Cuts at: 830 2245 2603 2919 3523 3598 4404 4515 4568
Size: 1415 358 316 604 75 806 111 53
Cuts at: 4568 830
Size: 3478
Fragments arranged by size:
3478 1415 806 604 358 316 111 75
53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7216
RsrII CG'GwC_CG
Cuts at: 2024 2024
Size: 7216
SacI G_AGCT'C
Cuts at: 2250 2250
Size: 7216
SacII CC_GC'GG
Cuts at: 2114 2124 2114
Size: 10 7206
SalI G'TCGA_C
Cuts at: 1936 4558 1936
Size: 2622 4594
SapI GCTCTTCn'nnn_
Cuts at: 4497 4497
Size: 7216
SbfI CC_TGCA'GG
Cuts at: 4568 4568
Size: 7216
ScaI AGT'ACT
Cuts at: 5464 7176 5464
Size: 1712 5504
SexAI A'CCwGG_T
Cuts at: 3813 3813
Size: 7216
SfcI C'TryA_G
Cuts at: 826 1045 2241 2599 2915 3227 3519 3594 4400
Size: 219 1196 358 316 312 292 75 806
Cuts at: 4400 4511 4564 5699 6380 6571 826
Size: 111 53 1135 681 191 1471
Fragments arranged by size:
1471 1196 1135 806 681 358 316 312
292 219 191 111 75 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7216
SmaI CCC'GGG
Cuts at: 1872 2104 4543 1872
Size: 232 2439 4545
Fragments arranged by size:
4545 2439 232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 2439 2460 3138 3314 3761
Size: 197 926 486 10 21 678 176 447
Cuts at: 3761 4154 5320 6221 6483 820
Size: 393 1166 901 262 1553
Fragments arranged by size:
1553 1166 926 901 678 486 447 393
262 197 176 21 10
SnaBI TAC'GTA
Cuts at: 493 5007 493
Size: 4514 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7216
SphI G_CATG'C
Cuts at: 1132 4574 1132
Size: 3442 3774
SspI AAT'ATT
Cuts at: 5 52 5140 5
Size: 47 5088 2081
Fragments arranged by size:
5088 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7216
StyI C'CwwG_G
Cuts at: 513 1065 2004 3282 3535 4286 4655 513
Size: 552 939 1278 253 751 369 3074
Fragments arranged by size:
3074 1278 939 751 552 369 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3249 3616 5243 5260 5413 5598 6043
Size: 1255 1669 367 1627 17 153 185 445
Cuts at: 6043 325
Size: 1498
Fragments arranged by size:
1669 1627 1498 1255 445 367 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2772 3426 5462
Size: 274 80 33 51 1443 795 654 2036
Cuts at: 5462 7174 96
Size: 1712 138
Fragments arranged by size:
2036 1712 1443 795 654 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7176 7208 7176
Size: 32 7184
Tth111I GACn'n_nGTC
Cuts at: 1316 4210 1316
Size: 2894 4322
XbaI T'CTAG_A
Cuts at: 4552 4552
Size: 7216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 7216
XhoI C'TCGA_G
Cuts at: 1943 2439 3138 1943
Size: 496 699 6021
Fragments arranged by size:
6021 699 496
XmaI C'CCGG_G
Cuts at: 1870 2102 4541 1870
Size: 232 2439 4545
Fragments arranged by size:
4545 2439 232
XmnI GAAnn'nnTTC
Cuts at: 5345 5345
Size: 7216
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3646 5024 276
Size: 53 83 186 936 2112 1378 2468
Fragments arranged by size:
2468 2112 1378 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PvuII SanDI SfiI
SfoI SrfI SwaI