Restriction map for
pFN21AA1760
(Circular) (Six-base) MAPSORT of: pFN21AA1760.seq Check: 7608 from: 1 to: 11335
pFN21AA1760 11335 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4245 5816 6937 8281 4245
Size: 1571 1121 1344 7299
Fragments arranged by size:
7299 1571 1344 1121
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3099 3537 4734 5067
Size: 53 83 186 936 1563 438 1197 333
Cuts at: 5067 6260 7281 9145 278
Size: 1193 1021 1864 2468
Fragments arranged by size:
2468 1864 1563 1197 1193 1021 936 438
333 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2589 7281 8678 1937
Size: 652 4692 1397 4594
Fragments arranged by size:
4692 4594 1397 652
Acc65I G'GTAC_C
Cuts at: 8318 8656 8318
Size: 338 10997
AclI AA'CG_TT
Cuts at: 1563 5696 9462 9835 1563
Size: 4133 3766 373 3063
Fragments arranged by size:
4133 3766 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2831 2843 2900 3440 4307 7697 7844 8207
Size: 1199 12 57 540 867 3390 147 363
Cuts at: 8207 8408 8429 9398 10782 11050 1632
Size: 201 21 969 1384 268 1917
Fragments arranged by size:
3390 1917 1384 1199 969 867 540 363
268 201 147 57 21 12
AfeI AGC'GCT
Cuts at: 2081 2262 2081
Size: 181 11154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11138
AflIII A'CryG_T
Cuts at: 1203 5486 5541 7015 10234 11211 1203
Size: 4283 55 1474 3219 977 1327
Fragments arranged by size:
4283 3219 1474 1327 977 55
AgeI A'CCGG_T
Cuts at: 10166 10166
Size: 11335
AhdI GACnn_n'nnGTC
Cuts at: 6019 10064 6019
Size: 4045 7290
AleI CACnn'nnGTG
Cuts at: 1166 3335 3528 1166
Size: 2169 193 8973
Fragments arranged by size:
8973 2169 193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4127 4341 7766 1184
Size: 2943 214 3425 4753
Fragments arranged by size:
4753 3425 2943 214
AlwNI CAG_nnn'CTG
Cuts at: 2173 2357 2878 3958 4192 5032 5808 6064 6200
Size: 184 521 1080 234 840 776 256 136
Cuts at: 6200 6238 6347 7660 8252 8425 9078 10650 2173
Size: 38 109 1313 592 173 653 1572 2858
Fragments arranged by size:
2858 1572 1313 1080 840 776 653 592
521 256 234 184 173 136 109 38
ApaI G_GGCC'C
Cuts at: 2031 2076 2156 3942 4124 4276 5268 6080 6525
Size: 45 80 1786 182 152 992 812 445
Cuts at: 6525 7403 7526 2031
Size: 878 123 5840
Fragments arranged by size:
5840 1786 992 878 812 445 182 152
123 80 45
ApaLI G'TGCA_C
Cuts at: 3224 9392 10548 3224
Size: 6168 1156 4011
Fragments arranged by size:
6168 4011 1156
ApoI r'AATT_y
Cuts at: 1493 8644 8870 9022 11131 1493
Size: 7151 226 152 2109 1697
Fragments arranged by size:
7151 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9889 160
Size: 872 8857 1606
Fragments arranged by size:
8857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11335
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2049 2309 2456 3044 3059 3713
Size: 73 69 37 260 147 588 15 654
Cuts at: 3713 3728 5267 5933 6251 6855 7131 7404 7506
Size: 15 1539 666 318 604 276 273 102
Cuts at: 7506 7526 7625 8490 8660 1870
Size: 20 99 865 170 4545
Fragments arranged by size:
4545 1539 865 666 654 604 588 318
276 273 260 170 147 102 99 73
69 37 20 15 15
AvrII C'CTAG_G
Cuts at: 8200 8200
Size: 11335
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4679 4712 4679
Size: 33 11302
BamHI G'GATC_C
Cuts at: 4063 8665 9032 4063
Size: 4602 367 6366
Fragments arranged by size:
6366 4602 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2865 3055 3667 4309
Size: 325 217 627 359 719 190 612 642
Cuts at: 4309 4561 4720 5426 5819 6097 7780 8318 8656
Size: 252 159 706 393 278 1683 538 338
Cuts at: 8656 10111 618
Size: 1455 1842
Fragments arranged by size:
1842 1683 1455 719 706 642 627 612
538 393 359 338 325 278 252 217
190 159
BanII G_rGCy'C
Cuts at: 1391 2013 2031 2076 2156 2284 2293 2316 2334
Size: 622 18 45 80 128 9 23 18
Cuts at: 2334 2457 2481 3351 3729 3827 3942 4016 4124
Size: 123 24 870 378 98 115 74 108
Cuts at: 4124 4276 4974 5157 5268 6080 6252 6384 6408
Size: 152 698 183 111 812 172 132 24
Cuts at: 6408 6525 6723 7026 7071 7374 7403 7526 7626
Size: 117 198 303 45 303 29 123 100
Cuts at: 7626 7950 8654 1391
Size: 324 704 4072
Fragments arranged by size:
4072 870 812 704 698 622 378 324
303 303 198 183 172 152 132 128
123 123 117 115 111 108 100 98
80 74 45 45 29 24 24 23
18 18 9
BbeI G_GCGC'C
Cuts at: 2150 3059 4313 4565 4724 5430 2150
Size: 909 1254 252 159 706 8055
Fragments arranged by size:
8055 1254 909 706 252 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2819 3411 8016 928
Size: 971 920 592 4605 4247
Fragments arranged by size:
4605 4247 971 920 592
BbvCI CC'TCA_GC
Cuts at: 5801 6667 6673 5801
Size: 866 6 10463
Fragments arranged by size:
10463 866 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 5627 5661 9526 9560 1903
Size: 34 2225 34 1431 34 3865 34 3678
Fragments arranged by size:
3865 3678 2225 1431 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9227 10437 9227
Size: 1210 10125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5263 5936 6075
Size: 107 122 71 631 605 3591 673 139
Cuts at: 6075 6113 6209 7740 9946 136
Size: 38 96 1531 2206 1525
Fragments arranged by size:
3591 2206 1531 1525 673 631 605 139
122 107 96 71 38
BglII A'GATC_T
Cuts at: 3174 7683 11330 3174
Size: 4509 3647 3179
Fragments arranged by size:
4509 3647 3179
BlpI GC'TnA_GC
Cuts at: 3305 4281 5744 5919 8044 8752 3305
Size: 976 1463 175 2125 708 5888
Fragments arranged by size:
5888 2125 1463 976 708 175
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2076 2156 2324 3228 3672 3942 4124
Size: 241 45 80 168 904 444 270 182
Cuts at: 4124 4276 4368 5214 5268 5822 6080 6100 6373
Size: 152 92 846 54 554 258 20 273
Cuts at: 6373 6525 6660 6881 7091 7403 7526 8583 9396
Size: 152 135 221 210 312 123 1057 813
Cuts at: 9396 10552 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1057 904 846 813 554 444
312 273 270 258 241 221 210 182
168 152 152 135 123 92 80 54
45 20
BmgBI CAC'GTC
Cuts at: 1318 6882 7312 8281 8513 1318
Size: 5564 430 969 232 4140
Fragments arranged by size:
5564 4140 969 430 232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2976 3901 5562
Size: 416 53 83 399 24 1559 925 1661
Cuts at: 5562 6773 7582 9066 10024 442
Size: 1211 809 1484 958 1753
Fragments arranged by size:
1753 1661 1559 1484 1211 958 925 809
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 7110 1056
Size: 6054 5281
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5347 6367 6814 7315 7521 5347
Size: 1020 447 501 206 9161
Fragments arranged by size:
9161 1020 501 447 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2684 3143 3488 4031 4202 4239 4280 4452
Size: 1445 459 345 543 171 37 41 172
Cuts at: 4452 4961 4989 5468 5600 6147 6318 6324 6575
Size: 509 28 479 132 547 171 6 251
Cuts at: 6575 6737 7071 7285 7861 7885 9995 1239
Size: 162 334 214 576 24 2110 2579
Fragments arranged by size:
2579 2110 1445 576 547 543 509 479
459 345 334 251 214 172 171 171
162 132 41 37 28 24 6
Bpu10I CC'TnA_GC
Cuts at: 2451 4968 5073 5151 5801 6065 6402 6667 6673
Size: 2517 105 78 650 264 337 265 6
Cuts at: 6673 7920 7970 8253 2451
Size: 1247 50 283 5533
Fragments arranged by size:
5533 2517 1247 650 337 283 265 264
105 78 50 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 2531 4314 7847 9460 10325 10623 2495
Size: 36 1783 3533 1613 865 298 3207
Fragments arranged by size:
3533 3207 1783 1613 865 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2603 2922 6802 7000 7405 9998 882
Size: 617 1104 319 3880 198 405 2593 2219
Fragments arranged by size:
3880 2593 2219 1104 617 405 319 198
BsaAI yAC'GTr
Cuts at: 493 1204 5652 6655 7016 9126 493
Size: 711 4448 1003 361 2110 2702
Fragments arranged by size:
4448 2702 2110 1003 711 361
BsaBI GATnn'nnATC
Cuts at: 11329 11329
Size: 11335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2236 2301 2909
Size: 53 83 186 936 614 89 65 608
Cuts at: 2909 3056 3096 3479 3534 4310 4562 4721 4731
Size: 147 40 383 55 776 252 159 10
Cuts at: 4731 5064 5427 5760 6257 6363 7278 7433 9142
Size: 333 363 333 497 106 915 155 1709
Cuts at: 9142 9524 10159 275
Size: 382 635 1451
Fragments arranged by size:
1709 1451 936 915 776 635 614 608
497 383 382 363 333 333 252 186
159 155 147 106 89 83 65 55
53 40 10
BsaWI w'CCGG_w
Cuts at: 2646 2957 5911 9768 10166 10440 10587 10998 2646
Size: 311 2954 3857 398 274 147 411 2983
Fragments arranged by size:
3857 2983 2954 411 398 311 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3269 3299 4423 4453 5756
Size: 30 165 30 1866 30 1124 30 1303
Cuts at: 5756 5786 6322 6352 8021 8051 1178
Size: 30 536 30 1669 30 4462
Fragments arranged by size:
4462 1866 1669 1303 1124 536 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2411 2501 2504 3086 3371 3746 4146
Size: 946 279 90 3 582 285 375 400
Cuts at: 4146 5336 5843 6112 6655 7336 1186
Size: 1190 507 269 543 681 5185
Fragments arranged by size:
5185 1190 946 681 582 543 507 400
375 285 279 269 90 3
BseYI C'CCAG_C
Cuts at: 2076 2563 3704 3967 4100 4167 4516 4674 4699
Size: 487 1141 263 133 67 349 158 25
Cuts at: 4699 5044 5171 6038 6080 6103 6407 6498 6677
Size: 345 127 867 42 23 304 91 179
Cuts at: 6677 6819 6974 7519 7535 7992 8265 8451 8521
Size: 142 155 545 16 457 273 186 70
Cuts at: 8521 10538 11034 2076
Size: 2017 496 2377
Fragments arranged by size:
2377 2017 1141 867 545 496 487 457
349 345 304 273 263 186 179 158
155 142 133 127 91 70 67 42
25 23 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2882 3937 3978 4778 5222 5864 6320
Size: 573 1146 1055 41 800 444 642 456
Cuts at: 6320 6381 6996 7442 8006 8261 8321 1163
Size: 61 615 446 564 255 60 4177
Fragments arranged by size:
4177 1146 1055 800 642 615 573 564
456 446 444 255 61 60 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 2412 2439 2539 4428 5767
Size: 275 186 68 343 27 100 1889 1339
Cuts at: 5767 8785 9546 10574 1540
Size: 3018 761 1028 2301
Fragments arranged by size:
3018 2301 1889 1339 1028 761 343 275
186 100 68 27
BsiHKAI G_wGCw'C
Cuts at: 3078 3228 3351 5157 5925 6252 6654 7626 8143
Size: 150 123 1806 768 327 402 972 517
Cuts at: 8143 9396 9481 10552 3078
Size: 1253 85 1071 3861
Fragments arranged by size:
3861 1806 1253 1071 972 768 517 402
327 150 123 85
BsmI GAATG_Cn'
Cuts at: 3169 8846 8939 3169
Size: 5677 93 5565
Fragments arranged by size:
5677 5565 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3470 6248 6264 6912 8198 8617 3470
Size: 2778 16 648 1286 419 6188
Fragments arranged by size:
6188 2778 1286 648 419 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2031 2076 2156 2284 2293 2316
Size: 399 223 18 45 80 128 9 23
Cuts at: 2316 2324 2334 2457 2481 3078 3228 3351 3672
Size: 8 10 123 24 597 150 123 321
Cuts at: 3672 3729 3827 3942 4016 4124 4276 4368 4974
Size: 57 98 115 74 108 152 92 606
Cuts at: 4974 5157 5214 5268 5822 5925 6080 6100 6252
Size: 183 57 54 554 103 155 20 152
Cuts at: 6252 6373 6384 6408 6525 6654 6660 6723 6881
Size: 121 11 24 117 129 6 63 158
Cuts at: 6881 7026 7071 7091 7374 7403 7526 7626 7950
Size: 145 45 20 283 29 123 100 324
Cuts at: 7950 8143 8583 8654 9396 9481 10552 1391
Size: 193 440 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 597 554 440 399
324 321 283 223 193 183 158 155
152 152 150 145 129 128 123 123
123 121 117 115 108 103 100 98
92 85 80 74 71 63 57 57
54 45 45 29 24 24 23 20
20 18 11 10 9 8 6
BspEI T'CCGG_A
Cuts at: 10998 10998
Size: 11335
BspHI T'CATG_A
Cuts at: 9224 10954 9224
Size: 1730 9605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3309 3667 4177 4245 4428 5469 5816 6937
Size: 2465 358 510 68 183 1041 347 1121
Cuts at: 6937 7003 7018 8281 8343 8690 844
Size: 66 15 1263 62 347 3489
Fragments arranged by size:
3489 2465 1263 1121 1041 510 358 347
347 183 68 66 62 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2668 4280 5072 5689 6744 7974 9222
Size: 429 845 1612 792 617 1055 1230 1248
Cuts at: 9222 1394
Size: 3507
Fragments arranged by size:
3507 1612 1248 1230 1055 845 792 617
429
BsrDI GCAATG_nn'
Cuts at: 65 1248 9830 10004 65
Size: 1183 8582 174 1396
Fragments arranged by size:
8582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2303 2868 3205 3664 4006 4222 4603
Size: 110 493 565 337 459 342 216 381
Cuts at: 4603 4786 4948 5098 5517 7511 9979 10166 1700
Size: 183 162 150 419 1994 2468 187 2869
Fragments arranged by size:
2869 2468 1994 565 493 459 419 381
342 337 216 187 183 162 150 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11335
BssHII G'CGCG_C
Cuts at: 1925 2224 5492 1925
Size: 299 3268 7768
Fragments arranged by size:
7768 3268 299
BssSI C'ACGA_G
Cuts at: 3524 6281 6308 8136 9395 10407 3524
Size: 2757 27 1828 1259 1012 4452
Fragments arranged by size:
4452 2757 1828 1259 1012 27
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 2769 2865 3757 3958 4915 5209 7660
Size: 1379 596 96 892 201 957 294 2451
Cuts at: 7660 794
Size: 4469
Fragments arranged by size:
4469 2451 1379 957 892 596 294 201
96
BstBI TT'CG_AA
Cuts at: 10139 10139
Size: 11335
BstEII G'GTnAC_C
Cuts at: 1184 3187 3685 5602 1184
Size: 2003 498 1917 6917
Fragments arranged by size:
6917 2003 1917 498
BstXI CCAn_nnnn'nTGG
Cuts at: 2530 3790 4556 5179 5656 7195 7543 8239 2530
Size: 1260 766 623 477 1539 348 696 5626
Fragments arranged by size:
5626 1539 1260 766 696 623 477 348
BstYI r'GATC_y
Cuts at: 1972 2874 3174 3597 4063 7683 8665 9032 9417
Size: 902 300 423 466 3620 982 367 385
Cuts at: 9417 9434 10886 10972 10984 11330 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3620 1977 1452 982 902 466 423 385
367 346 300 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2800 3389 6231 6786 2800
Size: 589 2842 555 7349
Fragments arranged by size:
7349 2842 589 555
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2295 2316 2517 2569 3090
Size: 552 939 27 264 21 201 52 521
Cuts at: 3090 3364 3987 4116 4206 4668 5184 5767 8544
Size: 274 623 129 90 462 516 583 2777
Cuts at: 8544 513
Size: 3304
Fragments arranged by size:
3304 2777 939 623 583 552 521 516
462 274 264 201 129 90 52 27
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2200 2332 3367 487
Size: 683 56 974 132 1035 8455
Fragments arranged by size:
8455 1035 974 683 132 56
BtsI GCAGTG_nn'
Cuts at: 4790 4808 4952 6477 7329 7748 7950 7989 8851
Size: 18 144 1525 852 419 202 39 862
Cuts at: 8851 9645 9665 4790
Size: 794 20 6460
Fragments arranged by size:
6460 1525 862 852 794 419 202 144
39 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3750 3785 529
Size: 35 3186 35 8079
Fragments arranged by size:
8079 3186 35 35
DraI TTT'AAA
Cuts at: 8639 8639
Size: 11335
DraIII CAC_nnn'GTG
Cuts at: 4756 4756
Size: 11335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6901 8119 10342 809
Size: 507 5585 1218 2223 1802
Fragments arranged by size:
5585 2223 1802 1218 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2436 2520
Size: 54 1425 50 275 18 236 370 84
Cuts at: 2520 2536 2997 3129 3207 3546 3817 4068 4144
Size: 16 461 132 78 339 271 251 76
Cuts at: 4144 4569 4605 4623 5038 5181 6489 7792 8071
Size: 425 36 18 415 143 1308 1303 279
Cuts at: 8071 8782 9671 11300 8
Size: 711 889 1629 43
Fragments arranged by size:
1629 1425 1308 1303 889 711 461 425
415 370 339 279 275 271 251 236
143 132 84 78 76 54 50 43
36 18 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2066 2436 2536 8782 1537
Size: 275 254 370 100 6246 4090
Fragments arranged by size:
6246 4090 370 275 254 100
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 2679 5885 9265 1353
Size: 1125 201 3206 3380 3423
Fragments arranged by size:
3423 3380 3206 1125 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 3651 8076 9922 10296 10442
Size: 1064 138 468 1594 4425 1846 374 146
Cuts at: 10442 387
Size: 1280
Fragments arranged by size:
4425 1846 1594 1280 1064 468 374 146
138
EcoICRI GAG'CTC
Cuts at: 3349 5155 6250 7624 3349
Size: 1806 1095 1374 7060
Fragments arranged by size:
7060 1806 1374 1095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2684 2831 2843 2900 3143 3440 3488
Size: 393 1052 147 12 57 243 297 48
Cuts at: 3488 4031 4202 4239 4280 4307 4452 4961 4989
Size: 543 171 37 41 27 145 509 28
Cuts at: 4989 5468 5600 6147 6318 6324 6575 6737 7071
Size: 479 132 547 171 6 251 162 334
Cuts at: 7071 7285 7697 7844 7861 7885 8207 8408 8429
Size: 214 412 147 17 24 322 201 21
Cuts at: 8429 9398 9995 10782 11050 1239
Size: 969 597 787 268 1524
Fragments arranged by size:
1524 1052 969 787 597 547 543 509
479 412 393 334 322 297 268 251
243 214 201 171 171 162 147 147
145 132 57 48 41 37 28 27
24 21 17 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3470 4184 6068 6355 7289 8256 8493 3470
Size: 714 1884 287 934 967 237 6312
Fragments arranged by size:
6312 1884 967 934 714 287 237
EcoO109I rG'GnC_Cy
Cuts at: 2027 2072 2073 2884 3938 3972 4189 4272 4273
Size: 45 1 811 1054 34 217 83 1
Cuts at: 4273 5077 5938 6077 6286 6423 6521 6681 6763
Size: 804 861 139 209 137 98 160 82
Cuts at: 6763 7143 7294 7399 7400 7523 7555 7910 7924
Size: 380 151 105 1 123 32 355 14
Cuts at: 7924 8223 8249 8261 2027
Size: 299 26 12 5101
Fragments arranged by size:
5101 1054 861 811 804 380 355 299
217 209 160 151 139 137 123 105
98 83 82 45 34 32 26 14
12 1 1 1
EcoRI G'AATT_C
Cuts at: 8644 9022 8644
Size: 378 10957
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5635 8338 5635
Size: 2703 8632
FspI TGC'GCA
Cuts at: 9841 9841
Size: 11335
HaeII r_GCGC'y
Cuts at: 1678 2083 2150 2182 2264 2757 3059 3783 3896
Size: 405 67 32 82 493 302 724 113
Cuts at: 3896 4313 4565 4724 5430 6005 7131 8528 10482
Size: 417 252 159 706 575 1126 1397 1954
Cuts at: 10482 1678
Size: 2531
Fragments arranged by size:
2531 1954 1397 1126 724 706 575 493
417 405 302 252 159 113 82 67
32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3025 3057 4423 4455 5756
Size: 32 135 32 1453 32 1366 32 1301
Cuts at: 5756 5788 6005 6037 6176 6208 7163 7195 7361
Size: 32 217 32 139 32 955 32 166
Cuts at: 7361 7393 7708 7740 8021 8053 8473 8497 8505
Size: 32 315 32 281 32 420 24 8
Cuts at: 8505 8529 9976 10008 10050 10082 1373
Size: 24 1447 32 42 32 2626
Fragments arranged by size:
2626 1453 1447 1366 1301 955 420 315
281 217 166 139 135 42 32 32
32 32 32 32 32 32 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 8679 8925 669
Size: 988 281 6741 246 3079
Fragments arranged by size:
6741 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11335
HpaI GTT'AAC
Cuts at: 8925 8925
Size: 11335
KasI G'GCGC_C
Cuts at: 2146 3055 4309 4561 4720 5426 2146
Size: 909 1254 252 159 706 8055
Fragments arranged by size:
8055 1254 909 706 252 159
KpnI G_GTAC'C
Cuts at: 8322 8660 8322
Size: 338 10997
MfeI C'AATT_G
Cuts at: 8934 8934
Size: 11335
MluI A'CGCG_T
Cuts at: 5486 11211 5486
Size: 5725 5610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 5799 6540 10449 10633 1305
Size: 783 3711 741 3909 184 2007
Fragments arranged by size:
3909 3711 2007 783 741 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 2999 3131 3548 4571
Size: 54 1425 343 690 477 132 417 1023
Cuts at: 4571 5040 5183 6491 8073 10
Size: 469 143 1308 1582 3272
Fragments arranged by size:
3272 1582 1425 1308 1023 690 477 469
417 343 143 132 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3081 3335 3528 4109 5654 9293 9652
Size: 648 1915 254 193 581 1545 3639 359
Cuts at: 9652 9811 518
Size: 159 2042
Fragments arranged by size:
3639 2042 1915 1545 648 581 359 254
193 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2297 2357 2571 2674 2862 3816 4162
Size: 427 264 60 214 103 188 954 346
Cuts at: 4162 4285 4703 5009 5254 5299 5418 5578 6019
Size: 123 418 306 245 45 119 160 441
Cuts at: 6019 6420 6677 6814 8386 8407 8751 9428 10576
Size: 401 257 137 1572 21 344 677 1148
Cuts at: 10576 10821 1606
Size: 245 2120
Fragments arranged by size:
2120 1572 1148 954 677 441 427 418
401 346 344 306 264 257 245 245
214 188 160 137 123 119 103 60
45 21
NaeI GCC'GGC
Cuts at: 2305 2870 3207 4788 4950 5519 7513 2305
Size: 565 337 1581 162 569 1994 6127
Fragments arranged by size:
6127 1994 1581 569 565 337 162
NarI GG'CG_CC
Cuts at: 2147 3056 4310 4562 4721 5427 2147
Size: 909 1254 252 159 706 8055
Fragments arranged by size:
8055 1254 909 706 252 159
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 9844
Fragments arranged by size:
9844 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11335
NgoMIV G'CCGG_C
Cuts at: 2303 2868 3205 4786 4948 5517 7511 2303
Size: 565 337 1581 162 569 1994 6127
Fragments arranged by size:
6127 1994 1581 569 565 337 162
NheI G'CTAG_C
Cuts at: 1052 7106 1052
Size: 6054 5281
NotI GC'GGCC_GC
Cuts at: 8782 8782
Size: 11335
NspI r_CATG'y
Cuts at: 1132 1229 3121 5743 8693 10238 1132
Size: 97 1892 2622 2950 1545 2229
Fragments arranged by size:
2950 2622 2229 1892 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2391 4556 5780 6517 6582 1348
Size: 1043 2165 1224 737 65 6101
Fragments arranged by size:
6101 2165 1224 1043 737 65
PciI A'CATG_T
Cuts at: 10234 10234
Size: 11335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5599 6826 1108
Size: 4491 1227 5617
Fragments arranged by size:
5617 4491 1227
PfoI T'CCnGG_A
Cuts at: 4059 8554 4059
Size: 4495 6840
PmeI GTTT'AAAC
Cuts at: 8639 8639
Size: 11335
PmlI CAC'GTG
Cuts at: 6655 7016 6655
Size: 361 10974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9404 9436 10917 10949 9404
Size: 32 1481 32 9790
Fragments arranged by size:
9790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4189 5077 6423 6681 6763 7143 7555 7924 8223
Size: 888 1346 258 82 380 412 369 299
Cuts at: 8223 4189
Size: 7301
Fragments arranged by size:
7301 1346 888 412 380 369 299 258
82
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11335
PsiI TTA'TAA
Cuts at: 8905 8905
Size: 11335
PspOMI G'GGCC_C
Cuts at: 2027 2072 2152 3938 4120 4272 5264 6076 6521
Size: 45 80 1786 182 152 992 812 445
Cuts at: 6521 7399 7522 2027
Size: 878 123 5840
Fragments arranged by size:
5840 1786 992 878 812 445 182 152
123 80 45
PspXI vC'TCGA_Gb
Cuts at: 6855 7131 7625 8490 6855
Size: 276 494 865 9700
Fragments arranged by size:
9700 865 494 276
PstI C_TGCA'G
Cuts at: 830 2178 2643 2712 3615 3678 4320 4533 5682
Size: 1348 465 69 903 63 642 213 1149
Cuts at: 5682 7497 7656 7698 7957 8687 830
Size: 1815 159 42 259 730 3478
Fragments arranged by size:
3478 1815 1348 1149 903 730 642 465
259 213 159 69 63 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11335
PvuII CAG'CTG
Cuts at: 2862 4285 5009 5299 5418 5578 6019 6677 6814
Size: 1423 724 290 119 160 441 658 137
Cuts at: 6814 8386 8407 2862
Size: 1572 21 5790
Fragments arranged by size:
5790 1572 1423 724 658 441 290 160
137 119 21
SacI G_AGCT'C
Cuts at: 3351 5157 6252 7626 3351
Size: 1806 1095 1374 7060
Fragments arranged by size:
7060 1806 1374 1095
SacII CC_GC'GG
Cuts at: 2034 2298 2572 2034
Size: 264 274 10797
Fragments arranged by size:
10797 274 264
SalI G'TCGA_C
Cuts at: 1936 8677 1936
Size: 6741 4594
SanDI GG'GwC_CC
Cuts at: 6423 6681 7143 6423
Size: 258 462 10615
Fragments arranged by size:
10615 462 258
SbfI CC_TGCA'GG
Cuts at: 3678 5682 8687 3678
Size: 2004 3005 6326
Fragments arranged by size:
6326 3005 2004
ScaI AGT'ACT
Cuts at: 9583 11295 9583
Size: 1712 9623
SexAI A'CCwGG_T
Cuts at: 8587 8587
Size: 11335
SfcI C'TryA_G
Cuts at: 826 1045 2174 2639 2708 3611 3674 4316 4529
Size: 219 1129 465 69 903 63 642 213
Cuts at: 4529 5678 7493 7652 7694 7953 8683 9818 10499
Size: 1149 1815 159 42 259 730 1135 681
Cuts at: 10499 10690 826
Size: 191 1471
Fragments arranged by size:
1815 1471 1149 1135 1129 903 730 681
642 465 259 219 213 191 159 69
63 42
SfoI GGC'GCC
Cuts at: 2148 3057 4311 4563 4722 5428 2148
Size: 909 1254 252 159 706 8055
Fragments arranged by size:
8055 1254 909 706 252 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11335
SgrAI Cr'CCGG_yG
Cuts at: 1700 3664 1700
Size: 1964 9371
SmaI CCC'GGG
Cuts at: 1872 2014 2458 3715 5269 8662 1872
Size: 142 444 1257 1554 3393 4545
Fragments arranged by size:
4545 3393 1554 1257 444 142
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 2546 4329 6251 6855 7131
Size: 197 926 567 36 1783 1922 604 276
Cuts at: 7131 7625 7862 8490 9439 10340 10602 820
Size: 494 237 628 949 901 262 1553
Fragments arranged by size:
1922 1783 1553 949 926 901 628 604
567 494 276 262 237 197 36
SnaBI TAC'GTA
Cuts at: 493 9126 493
Size: 8633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11335
SphI G_CATG'C
Cuts at: 1132 3121 8693 1132
Size: 1989 5572 3774
Fragments arranged by size:
5572 3774 1989
SrfI GCCC'GGGC
Cuts at: 2014 2458 3715 2014
Size: 444 1257 9634
Fragments arranged by size:
9634 1257 444
SspI AAT'ATT
Cuts at: 5 52 2947 9259 5
Size: 47 2895 6312 2081
Fragments arranged by size:
6312 2895 2081 47
StuI AGG'CCT
Cuts at: 1517 2706 5330 7501 1517
Size: 1189 2624 2171 5351
Fragments arranged by size:
5351 2624 2171 1189
StyI C'CwwG_G
Cuts at: 513 1065 2004 2757 2847 3432 3618 6374 6438
Size: 552 939 753 90 585 186 2756 64
Cuts at: 6438 6825 8074 8200 8774 513
Size: 387 1249 126 574 3074
Fragments arranged by size:
3074 2756 1249 939 753 585 574 552
387 186 126 90 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 2929 3388 5058 5231 7549 8109
Size: 1255 1025 324 459 1670 173 2318 560
Cuts at: 8109 8635 9362 9379 9532 9717 10162 325
Size: 526 727 17 153 185 445 1498
Fragments arranged by size:
2318 1670 1498 1255 1025 727 560 526
459 445 324 185 173 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3834 9581 11293
Size: 274 80 33 51 1443 1857 5747 1712
Cuts at: 11293 96
Size: 138
Fragments arranged by size:
5747 1857 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2904 2936 5465 5497 6322 6354 6933 6965 8536
Size: 32 2529 32 825 32 579 32 1571
Cuts at: 8536 8568 11295 11327 2904
Size: 32 2727 32 2912
Fragments arranged by size:
2912 2727 2529 1571 825 579 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 8572 1316
Size: 7256 4079
XbaI T'CTAG_A
Cuts at: 8671 8671
Size: 11335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3701 4378 5033 5656 6613 8236 3701
Size: 677 655 623 957 1623 6800
Fragments arranged by size:
6800 1623 957 677 655 623
XhoI C'TCGA_G
Cuts at: 1943 6251 6855 7131 7625 8490 1943
Size: 4308 604 276 494 865 4788
Fragments arranged by size:
4788 4308 865 604 494 276
XmaI C'CCGG_G
Cuts at: 1870 2012 2456 3713 5267 8660 1870
Size: 142 444 1257 1554 3393 4545
Fragments arranged by size:
4545 3393 1554 1257 444 142
XmnI GAAnn'nnTTC
Cuts at: 2828 9464 2828
Size: 6636 4699
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3097 3535 4732 5065
Size: 53 83 186 936 1563 438 1197 333
Cuts at: 5065 6258 7279 9143 276
Size: 1193 1021 1864 2468
Fragments arranged by size:
2468 1864 1563 1197 1193 1021 936 438
333 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BsiWI BstZ17I EcoRV FseI FspAI
NruI NsiI PacI PsrI RsrII SapI SfiI SwaI