Restriction map for
pFN21AA1757
(Circular) (Six-base) MAPSORT of: pFN21AA1757.seq Check: 6287 from: 1 to: 8698
pFN21AA1757 8698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 6508 278
Size: 53 83 186 936 2437 2535 2468
Fragments arranged by size:
2535 2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2120 3117 5868 6041 1937
Size: 183 997 2751 173 4594
Fragments arranged by size:
4594 2751 997 183 173
Acc65I G'GTAC_C
Cuts at: 6019 6019
Size: 8698
AclI AA'CG_TT
Cuts at: 1563 6825 7198 1563
Size: 5262 373 3063
Fragments arranged by size:
5262 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 3027 3372 3387 3672 5420 5440 5523
Size: 639 756 345 15 285 1748 20 83
Cuts at: 5523 6761 8145 8413 1632
Size: 1238 1384 268 1917
Fragments arranged by size:
1917 1748 1384 1238 756 639 345 285
268 83 20 15
AfeI AGC'GCT
Cuts at: 4019 4019
Size: 8698
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8501
AflIII A'CryG_T
Cuts at: 1203 3882 7597 8574 1203
Size: 2679 3715 977 1327
Fragments arranged by size:
3715 2679 1327 977
AgeI A'CCGG_T
Cuts at: 7529 7529
Size: 8698
AhdI GACnn_n'nnGTC
Cuts at: 5838 7427 5838
Size: 1589 7109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3597 1184
Size: 2413 6285
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2928 2960 2928
Size: 32 8666
AlwNI CAG_nnn'CTG
Cuts at: 3701 4008 4967 5306 5395 5959 6441 8013 3701
Size: 307 959 339 89 564 482 1572 4386
Fragments arranged by size:
4386 1572 959 564 482 339 307 89
ApaLI G'TGCA_C
Cuts at: 5936 6755 7911 5936
Size: 819 1156 6723
Fragments arranged by size:
6723 1156 819
ApoI r'AATT_y
Cuts at: 1493 2239 2260 2396 2660 3525 4912 5057 6007
Size: 746 21 136 264 865 1387 145 950
Cuts at: 6007 6233 6385 8494 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1387 950 865 746 264 226
152 145 136 21
AseI AT'TA_AT
Cuts at: 160 1032 2430 7252 160
Size: 872 1398 4822 1606
Fragments arranged by size:
4822 1606 1398 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8698
AvaI C'yCGr_G
Cuts at: 1870 1943 4653 6023 1870
Size: 73 2710 1370 4545
Fragments arranged by size:
4545 2710 1370 73
BamHI G'GATC_C
Cuts at: 3728 3853 4790 5021 6028 6395 3728
Size: 125 937 231 1007 367 6031
Fragments arranged by size:
6031 1007 937 367 231 125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5003 6019 7474 618
Size: 325 217 627 3216 1016 1455 1842
Fragments arranged by size:
3216 1842 1455 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4160 5262 6017 1391
Size: 2769 1102 755 4072
Fragments arranged by size:
4072 2769 1102 755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3432 928
Size: 971 1533 6194
Fragments arranged by size:
6194 1533 971
BbvCI CC'TCA_GC
Cuts at: 2820 5026 2820
Size: 2206 6492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4985 5019 6889 6923 1903
Size: 34 3048 34 1870 34 3678
Fragments arranged by size:
3678 3048 1870 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3106 4316 4427 4879 5477 6590 7800 3106
Size: 1210 111 452 598 1113 1210 4004
Fragments arranged by size:
4004 1210 1210 1113 598 452 111
BclI T'GATC_A
Cuts at: 1548 3103 1548
Size: 1555 7143
BfrBI ATG'CAT
Cuts at: 3229 3229
Size: 8698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3505 7309 136
Size: 107 122 71 631 605 1833 3804 1525
Fragments arranged by size:
3804 1833 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8693 8693
Size: 8698
BlpI GC'TnA_GC
Cuts at: 5338 6115 5338
Size: 777 7921
Bme1580I G_kGCm'C
Cuts at: 1790 3824 3836 5008 5940 6759 7915 1790
Size: 2034 12 1172 932 819 1156 2573
Fragments arranged by size:
2573 2034 1172 1156 932 819 12
BmgBI CAC'GTC
Cuts at: 1318 5562 1318
Size: 4244 4454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5841 6429 7387
Size: 416 53 83 399 24 4424 588 958
Cuts at: 7387 442
Size: 1753
Fragments arranged by size:
4424 1753 958 588 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5709 1056
Size: 4653 4045
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5314 5314
Size: 8698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3474 4212 4898 5463 5863 5936 7358 1239
Size: 2235 738 686 565 400 73 1422 2579
Fragments arranged by size:
2579 2235 1422 738 686 565 400 73
Bpu10I CC'TnA_GC
Cuts at: 2820 4905 5026 2820
Size: 2085 121 6492
Fragments arranged by size:
6492 2085 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5130 6823 7688 7986 5130
Size: 1693 865 298 5842
Fragments arranged by size:
5842 1693 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5503 7361 882
Size: 617 4004 1858 2219
Fragments arranged by size:
4004 2219 1858 617
BsaAI yAC'GTr
Cuts at: 493 1204 3842 6489 493
Size: 711 2638 2647 2702
Fragments arranged by size:
2702 2647 2638 711
BsaBI GATnn'nnATC
Cuts at: 4566 8692 4566
Size: 4126 4572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3970 6505 6887 7522
Size: 53 83 186 936 2437 2535 382 635
Cuts at: 7522 275
Size: 1451
Fragments arranged by size:
2535 2437 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2517 2895 7131 7529 7803 7950 8361 2517
Size: 378 4236 398 274 147 411 2854
Fragments arranged by size:
4236 2854 411 398 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2928 2958 4427 4457 5346
Size: 30 165 30 1525 30 1469 30 889
Cuts at: 5346 5376 5415 5445 5461 5491 5861 5891 5917
Size: 30 39 30 16 30 370 30 26
Cuts at: 5917 5947 1178
Size: 30 3929
Fragments arranged by size:
3929 1525 1469 889 370 165 39 30
30 30 30 30 30 30 30 30
26 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2946 3062 4322 4371 4497 4671 5698
Size: 922 838 116 1260 49 126 174 1027
Cuts at: 5698 1186
Size: 4186
Fragments arranged by size:
4186 1260 1027 922 838 174 126 116
49
BseYI C'CCAG_C
Cuts at: 2085 2286 2488 4269 4967 5852 7901 8397 2085
Size: 201 202 1781 698 885 2049 496 2386
Fragments arranged by size:
2386 2049 1781 885 698 496 202 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 2370 4923 5036 5487 1163
Size: 573 496 138 2553 113 451 4374
Fragments arranged by size:
4374 2553 573 496 451 138 113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3515 6148 6909 7937 1540
Size: 275 186 1514 2633 761 1028 2301
Fragments arranged by size:
2633 2301 1514 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2649 5940 6759 6844 7915 2649
Size: 3291 819 85 1071 3432
Fragments arranged by size:
3432 3291 1071 819 85
BsmI GAATG_Cn'
Cuts at: 4648 6209 6302 4648
Size: 1561 93 7044
Fragments arranged by size:
7044 1561 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3961 3961
Size: 8698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2649 3824 3836 4160 5008 5262 5940
Size: 399 859 1175 12 324 848 254 678
Cuts at: 5940 6017 6759 6844 7915 1391
Size: 77 742 85 1071 2174
Fragments arranged by size:
2174 1175 1071 859 848 742 678 399
324 254 85 77 12
BspEI T'CCGG_A
Cuts at: 8361 8361
Size: 8698
BspHI T'CATG_A
Cuts at: 3106 6587 8317 3106
Size: 3481 1730 3487
Fragments arranged by size:
3487 3481 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6053 844
Size: 5209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6585 1394
Size: 429 4762 3507
Fragments arranged by size:
4762 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4111 5813 7193 7367 65
Size: 1183 2863 1702 1380 174 1396
Fragments arranged by size:
2863 1702 1396 1380 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7342 7529 1700
Size: 110 5532 187 2869
Fragments arranged by size:
5532 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2356 2732 96
Size: 2260 376 6062
Fragments arranged by size:
6062 2260 376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8698
BssSI C'ACGA_G
Cuts at: 2502 4577 4785 6758 7770 2502
Size: 2075 208 1973 1012 3430
Fragments arranged by size:
3430 2075 1973 1012 208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8698
BstBI TT'CG_AA
Cuts at: 7502 7502
Size: 8698
BstEII G'GTnAC_C
Cuts at: 1184 2824 1184
Size: 1640 7058
BstYI r'GATC_y
Cuts at: 1972 2523 3728 3853 4676 4790 5021 6028 6395
Size: 551 1205 125 823 114 231 1007 367
Cuts at: 6395 6780 6797 8249 8335 8347 8693 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1205 1007 823 551 385 367
346 231 125 114 86 17 12
BstZ17I GTA'TAC
Cuts at: 2121 2121
Size: 8698
Bsu36I CC'TnA_GG
Cuts at: 2528 2528
Size: 8698
BtgI C'CryG_G
Cuts at: 513 1065 5008 5920 513
Size: 552 3943 912 3291
Fragments arranged by size:
3943 3291 912 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7959
Fragments arranged by size:
7959 683 56
BtsI GCAGTG_nn'
Cuts at: 2077 5134 5405 5957 6214 7008 7028 2077
Size: 3057 271 552 257 794 20 3747
Fragments arranged by size:
3747 3057 794 552 271 257 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8663
DraI TTT'AAA
Cuts at: 6002 6002
Size: 8698
DraIII CAC_nnn'GTG
Cuts at: 3416 4805 5587 5921 3416
Size: 1389 782 334 6193
Fragments arranged by size:
6193 1389 782 334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3564 7705 809
Size: 507 2248 4141 1802
Fragments arranged by size:
4141 2248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3512 4275 6145
Size: 54 1425 50 275 18 1682 763 1870
Cuts at: 6145 7034 8663 8
Size: 889 1629 43
Fragments arranged by size:
1870 1682 1629 1425 889 763 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3512 6145 1537
Size: 275 1700 2633 4090
Fragments arranged by size:
4090 2633 1700 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2488 2654 3138 3147 3333 3512 3612 4126
Size: 1135 166 484 9 186 179 100 514
Cuts at: 4126 4299 5560 6628 1353
Size: 173 1261 1068 3423
Fragments arranged by size:
3423 1261 1135 1068 514 484 186 179
173 166 100 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2053 7285 7659 7805 387
Size: 1064 138 464 5232 374 146 1280
Fragments arranged by size:
5232 1280 1064 464 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 3027 3372 3387 3474 3672 4212
Size: 393 639 756 345 15 87 198 540
Cuts at: 4212 4898 5420 5440 5463 5523 5863 5936 6761
Size: 686 522 20 23 60 340 73 825
Cuts at: 6761 7358 8145 8413 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 825 787 756 686 639 597 540
522 393 345 340 268 198 87 73
60 23 20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4737 4737
Size: 8698
EcoO109I rG'GnC_Cy
Cuts at: 3507 3978 4815 5221 5723 3507
Size: 471 837 406 502 6482
Fragments arranged by size:
6482 837 502 471 406
EcoRI G'AATT_C
Cuts at: 2239 6007 6385 2239
Size: 3768 378 4552
Fragments arranged by size:
4552 3768 378
FspI TGC'GCA
Cuts at: 7204 7204
Size: 8698
HaeII r_GCGC'y
Cuts at: 1678 4021 7845 1678
Size: 2343 3824 2531
Fragments arranged by size:
3824 2531 2343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 3050 3082 3720
Size: 32 135 32 467 32 979 32 638
Cuts at: 3720 3752 4069 4101 4314 4346 5196 5228 5824
Size: 32 317 32 213 32 850 32 596
Cuts at: 5824 5856 7339 7371 7413 7445 1373
Size: 32 1483 32 42 32 2626
Fragments arranged by size:
2626 1483 979 850 638 596 467 317
213 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3739 6042 6288 669
Size: 988 281 1801 2303 246 3079
Fragments arranged by size:
3079 2303 1801 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2328 748
Size: 1580 7118
HpaI GTT'AAC
Cuts at: 6288 6288
Size: 8698
KpnI G_GTAC'C
Cuts at: 6023 6023
Size: 8698
MfeI C'AATT_G
Cuts at: 6297 6297
Size: 8698
MluI A'CGCG_T
Cuts at: 8574 8574
Size: 8698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3881 4470 5019 7812 7996 1305
Size: 2576 589 549 2793 184 2007
Fragments arranged by size:
2793 2576 2007 589 549 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4277 10
Size: 54 1425 343 2445 4431
Fragments arranged by size:
4431 2445 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4713 6656 7015 7174 518
Size: 648 3547 1943 359 159 2042
Fragments arranged by size:
3547 2042 1943 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2488 4273 4458 5864 6114 6791 7939 8184
Size: 882 1785 185 1406 250 677 1148 245
Cuts at: 8184 1606
Size: 2120
Fragments arranged by size:
2120 1785 1406 1148 882 677 250 245
185
NcoI C'CATG_G
Cuts at: 513 1065 5008 513
Size: 552 3943 4203
Fragments arranged by size:
4203 3943 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8698
NheI G'CTAG_C
Cuts at: 1052 5705 1052
Size: 4653 4045
NotI GC'GGCC_GC
Cuts at: 6145 6145
Size: 8698
NsiI A_TGCA'T
Cuts at: 3231 3231
Size: 8698
NspI r_CATG'y
Cuts at: 1132 1229 3233 3886 6056 7601 1132
Size: 97 2004 653 2170 1545 2229
Fragments arranged by size:
2229 2170 2004 1545 653 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8698
PciI A'CATG_T
Cuts at: 3882 7597 3882
Size: 3715 4983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5651 5921 1108
Size: 4543 270 3885
Fragments arranged by size:
4543 3885 270
PfoI T'CCnGG_A
Cuts at: 5479 5479
Size: 8698
PmeI GTTT'AAAC
Cuts at: 6002 6002
Size: 8698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2928 2960 6767 6799 8280 8312 2928
Size: 32 3807 32 1481 32 3314
Fragments arranged by size:
3807 3314 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5221 5723 5221
Size: 502 8196
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8698
PsiI TTA'TAA
Cuts at: 2564 2865 6268 2564
Size: 301 3403 4994
Fragments arranged by size:
4994 3403 301
PspXI vC'TCGA_Gb
Cuts at: 4653 4653
Size: 8698
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2364 2396 2364
Size: 32 8666
PstI C_TGCA'G
Cuts at: 830 3466 4738 4963 5141 5311 5400 5721 6050
Size: 2636 1272 225 178 170 89 321 329
Cuts at: 6050 830
Size: 3478
Fragments arranged by size:
3478 2636 1272 329 321 225 178 170
89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8698
PvuII CAG'CTG
Cuts at: 2488 4273 4458 5864 2488
Size: 1785 185 1406 5322
Fragments arranged by size:
5322 1785 1406 185
SalI G'TCGA_C
Cuts at: 1936 6040 1936
Size: 4104 4594
SanDI GG'GwC_CC
Cuts at: 5723 5723
Size: 8698
SapI GCTCTTCn'nnn_
Cuts at: 2654 4126 2654
Size: 1472 7226
SbfI CC_TGCA'GG
Cuts at: 5721 6050 5721
Size: 329 8369
ScaI AGT'ACT
Cuts at: 6946 8658 6946
Size: 1712 6986
SexAI A'CCwGG_T
Cuts at: 3889 4801 3889
Size: 912 7786
SfcI C'TryA_G
Cuts at: 826 1045 2931 3462 4385 4734 4959 5137 5307
Size: 219 1886 531 923 349 225 178 170
Cuts at: 5307 5396 5717 6046 7181 7862 8053 826
Size: 89 321 329 1135 681 191 1471
Fragments arranged by size:
1886 1471 1135 923 681 531 349 329
321 225 219 191 178 170 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8698
SmaI CCC'GGG
Cuts at: 1872 6025 1872
Size: 4153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4653 5145 6802 7703 7965 820
Size: 197 926 2710 492 1657 901 262 1553
Fragments arranged by size:
2710 1657 1553 926 901 492 262 197
SnaBI TAC'GTA
Cuts at: 493 3842 6489 493
Size: 3349 2647 2702
Fragments arranged by size:
3349 2702 2647
SpeI A'CTAG_T
Cuts at: 152 3472 152
Size: 3320 5378
SphI G_CATG'C
Cuts at: 1132 3233 6056 1132
Size: 2101 2823 3774
Fragments arranged by size:
3774 2823 2101
SspI AAT'ATT
Cuts at: 5 52 6622 5
Size: 47 6570 2081
Fragments arranged by size:
6570 2081 47
StuI AGG'CCT
Cuts at: 1517 5876 1517
Size: 4359 4339
StyI C'CwwG_G
Cuts at: 513 1065 2811 3276 3612 5008 5217 5678 6137
Size: 552 1746 465 336 1396 209 461 459
Cuts at: 6137 513
Size: 3074
Fragments arranged by size:
3074 1746 1396 552 465 461 459 336
209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4311 6725 6742 6895 7080 7525 325
Size: 1255 2731 2414 17 153 185 445 1498
Fragments arranged by size:
2731 2414 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 2356 2732
Size: 274 80 33 51 1443 366 13 376
Cuts at: 2732 3862 6944 8656 96
Size: 1130 3082 1712 138
Fragments arranged by size:
3082 1712 1443 1130 376 366 274 138
80 51 33 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3838 3870 3942 3974 5415 5447 5573 5605 5776
Size: 32 72 32 1441 32 126 32 171
Cuts at: 5776 5808 5917 5949 8658 8690 3838
Size: 32 109 32 2709 32 3846
Fragments arranged by size:
3846 2709 1441 171 126 109 72 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8698
XbaI T'CTAG_A
Cuts at: 3664 6034 3664
Size: 2370 6328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4167 5599 5648 4167
Size: 1432 49 7217
Fragments arranged by size:
7217 1432 49
XhoI C'TCGA_G
Cuts at: 1943 4653 1943
Size: 2710 5988
XmaI C'CCGG_G
Cuts at: 1870 6023 1870
Size: 4153 4545
XmnI GAAnn'nnTTC
Cuts at: 6827 6827
Size: 8698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 6506 276
Size: 53 83 186 936 2437 2535 2468
Fragments arranged by size:
2535 2468 2437 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BsiWI BstXI
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI RsrII SacI SacII
SfiI SfoI SrfI SwaI