Restriction map for
pFN21AA1747
(Circular) (Six-base) MAPSORT of: pFN21AA1747.seq Check: 9185 from: 1 to: 7054
pFN21AA1747 7054 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3344 3344
Size: 7054
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4864 278
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4397 1937
Size: 2460 4594
Acc65I G'GTAC_C
Cuts at: 2235 4375 2235
Size: 2140 4914
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2311 2768 3092 3686 5117 6501 6769 1632
Size: 679 457 324 594 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 679 594 457 324 268
AfeI AGC'GCT
Cuts at: 2816 3200 2816
Size: 384 6670
AflII C'TTAA_G
Cuts at: 820 1017 4250 820
Size: 197 3233 3624
Fragments arranged by size:
3624 3233 197
AflIII A'CryG_T
Cuts at: 1203 5953 6930 1203
Size: 4750 977 1327
Fragments arranged by size:
4750 1327 977
AgeI A'CCGG_T
Cuts at: 2690 3054 5885 2690
Size: 364 2831 3859
Fragments arranged by size:
3859 2831 364
AhdI GACnn_n'nnGTC
Cuts at: 5783 5783
Size: 7054
AleI CACnn'nnGTG
Cuts at: 1166 2567 1166
Size: 1401 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3626 1184
Size: 2442 4612
AlwNI CAG_nnn'CTG
Cuts at: 2503 2791 3031 3127 3472 3538 4797 6369 2503
Size: 288 240 96 345 66 1259 1572 3188
Fragments arranged by size:
3188 1572 1259 345 288 240 96 66
ApaI G_GGCC'C
Cuts at: 2195 3368 3396 2195
Size: 1173 28 5853
Fragments arranged by size:
5853 1173 28
ApaLI G'TGCA_C
Cuts at: 3695 5111 6267 3695
Size: 1416 1156 4482
Fragments arranged by size:
4482 1416 1156
ApoI r'AATT_y
Cuts at: 1493 4024 4363 4589 4741 6850 1493
Size: 2531 339 226 152 2109 1697
Fragments arranged by size:
2531 2109 1697 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5608 160
Size: 872 4576 1606
Fragments arranged by size:
4576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 3448 4379 1870
Size: 73 189 1316 931 4545
Fragments arranged by size:
4545 1316 931 189 73
BamHI G'GATC_C
Cuts at: 3168 4278 4384 4751 3168
Size: 1110 106 367 5471
Fragments arranged by size:
5471 1110 367 106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2531 4375 5830 618
Size: 325 217 627 448 296 1844 1455 1842
Fragments arranged by size:
1844 1842 1455 627 448 325 296 217
BanII G_rGCy'C
Cuts at: 1391 2195 2868 3368 3396 3999 4173 4373 1391
Size: 804 673 500 28 603 174 200 4072
Fragments arranged by size:
4072 804 673 603 500 200 174 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3917 928
Size: 971 2018 4065
Fragments arranged by size:
4065 2018 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2123 2157 5245 5279 1903
Size: 34 186 34 3088 34 3678
Fragments arranged by size:
3678 3088 186 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7054
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2088 2117
Size: 107 122 71 631 605 400 16 29
Cuts at: 2117 2663 5665 136
Size: 546 3002 1525
Fragments arranged by size:
3002 1525 631 605 546 400 122 107
71 29 16
BglII A'GATC_T
Cuts at: 3723 7049 3723
Size: 3326 3728
BlpI GC'TnA_GC
Cuts at: 4471 4471
Size: 7054
Bme1580I G_kGCm'C
Cuts at: 1790 2195 3368 3396 3406 3699 5115 6271 1790
Size: 405 1173 28 10 293 1416 1156 2573
Fragments arranged by size:
2573 1416 1173 1156 405 293 28 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2087 2513 3076
Size: 416 53 83 399 24 670 426 563
Cuts at: 3076 3850 3865 4785 5743 442
Size: 774 15 920 958 1753
Fragments arranged by size:
1753 958 920 774 670 563 426 416
399 83 53 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3604 3604
Size: 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2597 3399 3539 3560 3661 3770 4022 4094
Size: 1358 802 140 21 101 109 252 72
Cuts at: 4094 5714 1239
Size: 1620 2579
Fragments arranged by size:
2579 1620 1358 802 252 140 109 101
72 21
Bpu10I CC'TnA_GC
Cuts at: 2208 4172 2208
Size: 1964 5090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4116 5179 6044 6342 4116
Size: 1063 865 298 4828
Fragments arranged by size:
4828 1063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2380 2573 2675 4328 5717 882
Size: 617 881 193 102 1653 1389 2219
Fragments arranged by size:
2219 1653 1389 881 617 193 102
BsaAI yAC'GTr
Cuts at: 493 1204 3700 4845 493
Size: 711 2496 1145 2702
Fragments arranged by size:
2702 2496 1145 711
BsaBI GATnn'nnATC
Cuts at: 7048 7048
Size: 7054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4861 5243 5878 275
Size: 53 83 186 936 3328 382 635 1451
Fragments arranged by size:
3328 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2361 2690 3054 5487 5885 6159 6306 6717 2361
Size: 329 364 2433 398 274 147 411 2698
Fragments arranged by size:
2698 2433 411 398 364 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3533 3563 4211 4241 1178
Size: 30 165 30 2130 30 648 30 3991
Fragments arranged by size:
3991 2130 648 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 3266 1186
Size: 1292 788 4974
Fragments arranged by size:
4974 1292 788
BseYI C'CCAG_C
Cuts at: 2091 3127 3616 6257 6753 2091
Size: 1036 489 2641 496 2392
Fragments arranged by size:
2641 2392 1036 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 2624 2920 3316 4134 1163
Size: 573 834 54 296 396 818 4083
Fragments arranged by size:
4083 834 818 573 396 296 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2045 4504 5265 6293 1540
Size: 275 186 44 2459 761 1028 2301
Fragments arranged by size:
2459 2301 1028 761 275 186 44
BsiHKAI G_wGCw'C
Cuts at: 2627 2868 3415 3699 4173 5115 5200 6271 2627
Size: 241 547 284 474 942 85 1071 3410
Fragments arranged by size:
3410 1071 942 547 474 284 241 85
BsmI GAATG_Cn'
Cuts at: 2924 4565 4658 2924
Size: 1641 93 5320
Fragments arranged by size:
5320 1641 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2627 2868 3368 3396 3406 3415
Size: 399 405 432 241 500 28 10 9
Cuts at: 3415 3699 3999 4173 4373 5115 5200 6271 1391
Size: 284 300 174 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 432 405 399 300
284 241 200 174 85 28 10 9
BspEI T'CCGG_A
Cuts at: 2361 6717 2361
Size: 4356 2698
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3344 3561 4045 4409 844
Size: 2500 217 484 364 3489
Fragments arranged by size:
3489 2500 484 364 217
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 4941 1394
Size: 429 410 2708 3507
Fragments arranged by size:
3507 2708 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 2336 3610 5549 5723 65
Size: 1183 1088 1274 1939 174 1396
Fragments arranged by size:
1939 1396 1274 1183 1088 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2690 2997 3054 3177 3477 4337
Size: 110 234 646 307 57 123 300 860
Cuts at: 4337 5698 5885 1700
Size: 1361 187 2869
Fragments arranged by size:
2869 1361 860 646 307 300 234 187
123 110 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 5114 6126 5114
Size: 1012 6042
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3201 794
Size: 2407 4647
BstBI TT'CG_AA
Cuts at: 5858 5858
Size: 7054
BstEII G'GTnAC_C
Cuts at: 1184 2674 1184
Size: 1490 5564
BstXI CCAn_nnnn'nTGG
Cuts at: 2488 2488
Size: 7054
BstYI r'GATC_y
Cuts at: 1972 2970 3168 3723 4278 4384 4751 5136 5153
Size: 998 198 555 555 106 367 385 17
Cuts at: 5153 6605 6691 6703 7049 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 998 555 555 385 367 346
198 106 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4292 4292
Size: 7054
BtgI C'CryG_G
Cuts at: 513 1065 2057 2112 2658 2949 3093 513
Size: 552 992 55 546 291 144 4474
Fragments arranged by size:
4474 992 552 546 291 144 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3191 487
Size: 683 56 1965 4350
Fragments arranged by size:
4350 1965 683 56
BtsI GCAGTG_nn'
Cuts at: 2021 2557 4570 5364 5384 2021
Size: 536 2013 794 20 3691
Fragments arranged by size:
3691 2013 794 536 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4305 4340 529
Size: 35 3741 35 3243
Fragments arranged by size:
3741 3243 35 35
DraI TTT'AAA
Cuts at: 4358 4358
Size: 7054
DraIII CAC_nnn'GTG
Cuts at: 3434 3434
Size: 7054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6061 809
Size: 507 4745 1802
Fragments arranged by size:
4745 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2042 2155 2595
Size: 54 1425 50 275 18 212 113 440
Cuts at: 2595 3479 3489 3762 4257 4501 5390 7019 8
Size: 884 10 273 495 244 889 1629 43
Fragments arranged by size:
1629 1425 889 884 495 440 275 273
244 212 113 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2042 4501 1537
Size: 275 230 2459 4090
Fragments arranged by size:
4090 2459 275 230
EarI CTCTTCn'nnn_
Cuts at: 1353 2463 3003 3012 3188 3662 4984 1353
Size: 1110 540 9 176 474 1322 3423
Fragments arranged by size:
3423 1322 1110 540 474 176 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3212 5641 6015 6161 387
Size: 1064 138 1623 2429 374 146 1280
Fragments arranged by size:
2429 1623 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2866 4171 2866
Size: 1305 5749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2597 2768 3092 3399 3539 3560
Size: 393 679 286 171 324 307 140 21
Cuts at: 3560 3661 3686 3770 4022 4094 5117 5714 6501
Size: 101 25 84 252 72 1023 597 787
Cuts at: 6501 6769 1239
Size: 268 1524
Fragments arranged by size:
1524 1023 787 679 597 393 324 307
286 268 252 171 140 101 84 72
25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3230 4291 3230
Size: 1061 5993
EcoO109I rG'GnC_Cy
Cuts at: 2191 2192 3028 3240 3364 3365 3392 3535 2191
Size: 1 836 212 124 1 27 143 5710
Fragments arranged by size:
5710 836 212 143 124 27 1 1
EcoRI G'AATT_C
Cuts at: 4363 4741 4363
Size: 378 6676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2341 3934 4232 2341
Size: 1593 298 5163
Fragments arranged by size:
5163 1593 298
FspI TGC'GCA
Cuts at: 5560 5560
Size: 7054
HaeII r_GCGC'y
Cuts at: 1678 2818 3202 6201 1678
Size: 1140 384 2999 2531
Fragments arranged by size:
2999 2531 1140 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 2680 2712 3956
Size: 32 135 32 805 32 271 32 1244
Cuts at: 3956 3988 3997 4029 4211 4243 5695 5727 5769
Size: 32 9 32 182 32 1452 32 42
Cuts at: 5769 5801 1373
Size: 32 2626
Fragments arranged by size:
2626 1452 1244 805 271 182 135 42
32 32 32 32 32 32 32 32
32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3775 4398 4644 669
Size: 988 281 1837 623 246 3079
Fragments arranged by size:
3079 1837 988 623 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7054
HpaI GTT'AAC
Cuts at: 4644 4644
Size: 7054
KpnI G_GTAC'C
Cuts at: 2239 4379 2239
Size: 2140 4914
MfeI C'AATT_G
Cuts at: 4653 4653
Size: 7054
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2267 4095 4237 6168 6352 1305
Size: 962 1828 142 1931 184 2007
Fragments arranged by size:
2007 1931 1828 962 184 142
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 3764 4259 10
Size: 54 1425 343 325 1607 495 2805
Fragments arranged by size:
2805 1607 1425 495 343 325 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 2567 3815 5012 5371 5530 518
Size: 648 1320 81 1248 1197 359 159 2042
Fragments arranged by size:
2042 1320 1248 1197 648 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 2567 2593 3127 3330 3624 3866 4470 5147
Size: 961 26 534 203 294 242 604 677
Cuts at: 5147 6295 6540 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 961 677 604 534 294 245
242 203 26
NaeI GCC'GGC
Cuts at: 2046 3179 3479 2046
Size: 1133 300 5621
Fragments arranged by size:
5621 1133 300
NcoI C'CATG_G
Cuts at: 513 1065 2057 2949 3093 513
Size: 552 992 892 144 4474
Fragments arranged by size:
4474 992 892 552 144
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7054
NgoMIV G'CCGG_C
Cuts at: 2044 3177 3477 2044
Size: 1133 300 5621
Fragments arranged by size:
5621 1133 300
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7054
NotI GC'GGCC_GC
Cuts at: 4501 4501
Size: 7054
NspI r_CATG'y
Cuts at: 1132 1229 2144 2850 3613 3655 4412 5957 1132
Size: 97 915 706 763 42 757 1545 2229
Fragments arranged by size:
2229 1545 915 763 757 706 97 42
PasI CC'CwG_GG
Cuts at: 1348 3466 1348
Size: 2118 4936
PciI A'CATG_T
Cuts at: 5953 5953
Size: 7054
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2950 3127 1108
Size: 902 940 177 5035
Fragments arranged by size:
5035 940 902 177
PfoI T'CCnGG_A
Cuts at: 2973 3312 3638 4095 2973
Size: 339 326 457 5932
Fragments arranged by size:
5932 457 339 326
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PmlI CAC'GTG
Cuts at: 3700 3700
Size: 7054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5123 5155 6636 6668 3791
Size: 32 1300 32 1481 32 4177
Fragments arranged by size:
4177 1481 1300 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3240 3535 3240
Size: 295 6759
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7054
PsiI TTA'TAA
Cuts at: 4624 4624
Size: 7054
PspOMI G'GGCC_C
Cuts at: 2191 3364 3392 2191
Size: 1173 28 5853
Fragments arranged by size:
5853 1173 28
PstI C_TGCA'G
Cuts at: 830 2708 2830 2970 3561 4406 830
Size: 1878 122 140 591 845 3478
Fragments arranged by size:
3478 1878 845 591 140 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
PvuII CAG'CTG
Cuts at: 2567 2593 3127 3330 3624 3866 2567
Size: 26 534 203 294 242 5755
Fragments arranged by size:
5755 534 294 242 203 26
SacI G_AGCT'C
Cuts at: 2868 4173 2868
Size: 1305 5749
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SapI GCTCTTCn'nnn_
Cuts at: 3188 3662 3188
Size: 474 6580
SbfI CC_TGCA'GG
Cuts at: 4406 4406
Size: 7054
ScaI AGT'ACT
Cuts at: 5302 7014 5302
Size: 1712 5342
SexAI A'CCwGG_T
Cuts at: 2255 2255
Size: 7054
SfcI C'TryA_G
Cuts at: 826 1045 2704 2826 2966 3557 4066 4348 4402
Size: 219 1659 122 140 591 509 282 54
Cuts at: 4402 5537 6218 6409 826
Size: 1135 681 191 1471
Fragments arranged by size:
1659 1471 1135 681 591 509 282 219
191 140 122 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7054
SmaI CCC'GGG
Cuts at: 1872 3450 4381 1872
Size: 1578 931 4545
Fragments arranged by size:
4545 1578 931
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4131 4250 5158 6059 6321 820
Size: 197 926 2188 119 908 901 262 1553
Fragments arranged by size:
2188 1553 926 908 901 262 197 119
SnaBI TAC'GTA
Cuts at: 493 4845 493
Size: 4352 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 2144 3655 4412 1132
Size: 1012 1511 757 3774
Fragments arranged by size:
3774 1511 1012 757
SspI AAT'ATT
Cuts at: 5 52 4978 5
Size: 47 4926 2081
Fragments arranged by size:
4926 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7054
StyI C'CwwG_G
Cuts at: 513 1065 2057 2377 2458 2949 3093 3231 3381
Size: 552 992 320 81 491 144 138 150
Cuts at: 3381 3894 4493 513
Size: 513 599 3074
Fragments arranged by size:
3074 992 599 552 513 491 320 150
144 138 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2083 5081 5098 5251 5436 5881 325
Size: 1255 503 2998 17 153 185 445 1498
Fragments arranged by size:
2998 1498 1255 503 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2767 3678 5300
Size: 274 80 33 51 1443 790 911 1622
Cuts at: 5300 7012 96
Size: 1712 138
Fragments arranged by size:
1712 1622 1443 911 790 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7014 7046 7014
Size: 32 7022
Tth111I GACn'n_nGTC
Cuts at: 1316 2729 1316
Size: 1413 5641
XbaI T'CTAG_A
Cuts at: 4390 4390
Size: 7054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4267 4267
Size: 7054
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7054
XmaI C'CCGG_G
Cuts at: 1870 3448 4379 1870
Size: 1578 931 4545
Fragments arranged by size:
4545 1578 931
XmnI GAAnn'nnTTC
Cuts at: 2321 3507 3914 5183 2321
Size: 1186 407 1269 4192
Fragments arranged by size:
4192 1269 1186 407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4862 276
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI