Restriction map for
pFN21AA1739
(Circular) (Six-base) MAPSORT of: pFN21AA1739.seq Check: 5181 from: 1 to: 7204
pFN21AA1739 7204 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2749 3202 2749
Size: 453 6751
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5014 278
Size: 53 83 186 936 3478 2468
Fragments arranged by size:
3478 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2709 4547 1937
Size: 772 1838 4594
Fragments arranged by size:
4594 1838 772
Acc65I G'GTAC_C
Cuts at: 4525 4525
Size: 7204
AclI AA'CG_TT
Cuts at: 1563 5331 5704 1563
Size: 3768 373 3063
Fragments arranged by size:
3768 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2215 2704 2861 3627 3939 5267 6651 6919
Size: 583 489 157 766 312 1328 1384 268
Cuts at: 6919 1632
Size: 1917
Fragments arranged by size:
1917 1384 1328 766 583 489 312 268
157
AflII C'TTAA_G
Cuts at: 820 1017 4232 820
Size: 197 3215 3792
Fragments arranged by size:
3792 3215 197
AflIII A'CryG_T
Cuts at: 1203 2973 6103 7080 1203
Size: 1770 3130 977 1327
Fragments arranged by size:
3130 1770 1327 977
AgeI A'CCGG_T
Cuts at: 6035 6035
Size: 7204
AhdI GACnn_n'nnGTC
Cuts at: 5933 5933
Size: 7204
AleI CACnn'nnGTG
Cuts at: 1166 3704 1166
Size: 2538 4666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3467 1184
Size: 2283 4921
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 2559
Size: 32 7172
AlwNI CAG_nnn'CTG
Cuts at: 3579 3838 4312 4471 4947 6519 3579
Size: 259 474 159 476 1572 4264
Fragments arranged by size:
4264 1572 476 474 259 159
ApaI G_GGCC'C
Cuts at: 2595 3035 3372 3976 4278 2595
Size: 440 337 604 302 5521
Fragments arranged by size:
5521 604 440 337 302
ApaLI G'TGCA_C
Cuts at: 2413 5261 6417 2413
Size: 2848 1156 3200
Fragments arranged by size:
3200 2848 1156
ApoI r'AATT_y
Cuts at: 1493 4513 4739 4891 7000 1493
Size: 3020 226 152 2109 1697
Fragments arranged by size:
3020 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5758 160
Size: 872 4726 1606
Fragments arranged by size:
4726 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7204
AvaI C'yCGr_G
Cuts at: 1870 1943 2211 3540 3803 3969 4409 4487 4529
Size: 73 268 1329 263 166 440 78 42
Cuts at: 4529 1870
Size: 4545
Fragments arranged by size:
4545 1329 440 268 263 166 78 73
42
BamHI G'GATC_C
Cuts at: 4198 4534 4901 4198
Size: 336 367 6501
Fragments arranged by size:
6501 367 336
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2390 3132 3254 4257
Size: 325 217 627 420 183 742 122 1003
Cuts at: 4257 4413 4525 5980 618
Size: 156 112 1455 1842
Fragments arranged by size:
1842 1455 1003 742 627 420 325 217
183 156 122 112
BanII G_rGCy'C
Cuts at: 1391 2187 2595 3035 3372 3417 3976 4149 4278
Size: 796 408 440 337 45 559 173 129
Cuts at: 4278 4410 4431 4523 1391
Size: 132 21 92 4072
Fragments arranged by size:
4072 796 559 440 408 337 173 132
129 92 45 21
BbeI G_GCGC'C
Cuts at: 2211 3136 2211
Size: 925 6279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 2297 2615 928
Size: 971 198 200 318 5517
Fragments arranged by size:
5517 971 318 200 198
BbvCI CC'TCA_GC
Cuts at: 2067 2067
Size: 7204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2732 2766 3933 3967 5395 5429 1903
Size: 34 795 34 1167 34 1428 34 3678
Fragments arranged by size:
3678 1428 1167 795 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2312 5096 6306 2312
Size: 2784 1210 3210
Fragments arranged by size:
3210 2784 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7204
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2191 2216 2508
Size: 107 122 71 631 605 519 25 292
Cuts at: 2508 4022 5815 136
Size: 1514 1793 1525
Fragments arranged by size:
1793 1525 1514 631 605 519 292 122
107 71 25
BglII A'GATC_T
Cuts at: 7199 7199
Size: 7204
BlpI GC'TnA_GC
Cuts at: 4621 4621
Size: 7204
Bme1580I G_kGCm'C
Cuts at: 1790 2417 2564 2595 3035 3257 3372 3716 3976
Size: 627 147 31 440 222 115 344 260
Cuts at: 3976 4278 4416 5265 6421 1790
Size: 302 138 849 1156 2573
Fragments arranged by size:
2573 1156 849 627 440 344 302 260
222 147 138 115 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7204
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 2199 4935
Size: 416 53 83 399 24 609 173 2736
Cuts at: 4935 5893 442
Size: 958 1753
Fragments arranged by size:
2736 1753 958 609 416 399 173 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 7204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 3176 3580 3815 4106 4151 4275 5864
Size: 1028 909 404 235 291 45 124 1589
Cuts at: 5864 1239
Size: 2579
Fragments arranged by size:
2579 1589 1028 909 404 291 235 124
45
Bpu10I CC'TnA_GC
Cuts at: 2067 2496 4425 2067
Size: 429 1929 4846
Fragments arranged by size:
4846 1929 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3335 5329 6194 6492 3335
Size: 1994 865 298 4047
Fragments arranged by size:
4047 1994 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5867 882
Size: 617 4368 2219
Fragments arranged by size:
4368 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2203 4372 4995 493
Size: 711 999 2169 623 2702
Fragments arranged by size:
2702 2169 999 711 623
BsaBI GATnn'nnATC
Cuts at: 7198 7198
Size: 7204
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 3133 5011 5393
Size: 53 83 186 936 675 925 1878 382
Cuts at: 5393 6028 275
Size: 635 1451
Fragments arranged by size:
1878 1451 936 925 675 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5637 6035 6309 6456 6867 5637
Size: 398 274 147 411 5974
Fragments arranged by size:
5974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2354 2384 3134 3164 3728
Size: 30 165 30 951 30 750 30 564
Cuts at: 3728 3758 1178
Size: 30 4624
Fragments arranged by size:
4624 951 750 564 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3221 3404 3733 3908 4505 1186
Size: 2035 183 329 175 597 3885
Fragments arranged by size:
3885 2035 597 329 183 175
BseYI C'CCAG_C
Cuts at: 2491 3092 3855 6407 6903 2491
Size: 601 763 2552 496 2792
Fragments arranged by size:
2792 2552 763 601 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3503 3543 1163
Size: 573 1767 40 4824
Fragments arranged by size:
4824 1767 573 40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4654 5415 6443 1540
Size: 275 186 1282 1371 761 1028 2301
Fragments arranged by size:
2301 1371 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2417 4431 5265 5350 6421 2417
Size: 2014 834 85 1071 3200
Fragments arranged by size:
3200 2014 1071 834 85
BsmI GAATG_Cn'
Cuts at: 4715 4808 4715
Size: 93 7111
BsmBI CGTCTCn'nnnn_
Cuts at: 3330 4438 3330
Size: 1108 6096
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2417 2564 2595 3035 3257 3372
Size: 399 397 230 147 31 440 222 115
Cuts at: 3372 3417 3716 3976 4149 4278 4410 4416 4431
Size: 45 299 260 173 129 132 6 15
Cuts at: 4431 4523 5265 5350 6421 1391
Size: 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 440 399 397 299 260
230 222 173 147 132 129 115 92
85 45 31 15 6
BspEI T'CCGG_A
Cuts at: 6867 6867
Size: 7204
BspHI T'CATG_A
Cuts at: 5093 6823 5093
Size: 1730 5474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2749 3202 3795 4559 844
Size: 1905 453 593 764 3489
Fragments arranged by size:
3489 1905 764 593 453
BsrBI CCG'CTC
Cuts at: 1394 1823 2728 3471 5091 1394
Size: 429 905 743 1620 3507
Fragments arranged by size:
3507 1620 905 743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4058 5699 5873 65
Size: 1183 2810 1641 174 1396
Fragments arranged by size:
2810 1641 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 3461 4065 5848 6035 1700
Size: 110 337 1314 604 1783 187 2869
Fragments arranged by size:
2869 1783 1314 604 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7204
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7204
BssSI C'ACGA_G
Cuts at: 2769 3109 3242 5264 6276 2769
Size: 340 133 2022 1012 3697
Fragments arranged by size:
3697 2022 1012 340 133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2861 2902 2911 4420 794
Size: 2067 41 9 1509 3578
Fragments arranged by size:
3578 2067 1509 41 9
BstBI TT'CG_AA
Cuts at: 6008 6008
Size: 7204
BstEII G'GTnAC_C
Cuts at: 1184 3955 1184
Size: 2771 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 3154 4268 3154
Size: 1114 6090
BstYI r'GATC_y
Cuts at: 1972 3012 3605 4198 4534 4901 5286 5303 6755
Size: 1040 593 593 336 367 385 17 1452
Cuts at: 6755 6841 6853 7199 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1040 593 593 385 367 346
336 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2280 3136 3492 3588 3661
Size: 552 939 117 159 856 356 96 73
Cuts at: 3661 513
Size: 4056
Fragments arranged by size:
4056 939 856 552 356 159 117 96
73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2915 487
Size: 683 56 1689 4776
Fragments arranged by size:
4776 1689 683 56
BtsI GCAGTG_nn'
Cuts at: 2739 3880 4469 4720 5514 5534 2739
Size: 1141 589 251 794 20 4409
Fragments arranged by size:
4409 1141 794 589 251 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2926 2945 2961 2980 3643 3678 529
Size: 35 2362 19 16 19 663 35 4055
Fragments arranged by size:
4055 2362 663 35 35 19 19 16
DraI TTT'AAA
Cuts at: 4508 4508
Size: 7204
DraIII CAC_nnn'GTG
Cuts at: 3274 3274
Size: 7204
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2705 6211 809
Size: 507 1389 3506 1802
Fragments arranged by size:
3506 1802 1389 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2538 2985
Size: 54 1425 50 275 18 288 420 447
Cuts at: 2985 3276 3495 3585 3595 4651 5540 7169 8
Size: 291 219 90 10 1056 889 1629 43
Fragments arranged by size:
1629 1425 1056 889 447 420 291 288
275 219 90 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 4651 1537
Size: 275 2839 4090
Fragments arranged by size:
4090 2839 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3003 4074 5134 1353
Size: 1650 1071 1060 3423
Fragments arranged by size:
3423 1650 1071 1060
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 5791 6165 6311 387
Size: 1064 138 2774 1428 374 146 1280
Fragments arranged by size:
2774 1428 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4429 4429
Size: 7204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2267 2704 2861 3176 3580 3627
Size: 393 583 52 437 157 315 404 47
Cuts at: 3627 3815 3939 4106 4151 4275 5267 5864 6651
Size: 188 124 167 45 124 992 597 787
Cuts at: 6651 6919 1239
Size: 268 1524
Fragments arranged by size:
1524 992 787 597 583 437 404 393
315 268 188 167 157 124 124 52
47 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3386 3386
Size: 7204
EcoO109I rG'GnC_Cy
Cuts at: 2050 2218 2242 2275 2591 2592 3031 3032 3218
Size: 168 24 33 316 1 439 1 186
Cuts at: 3218 3368 3369 3544 3784 3807 3835 3873 3973
Size: 150 1 175 240 23 28 38 100
Cuts at: 3973 4113 4274 4275 2050
Size: 140 161 1 4979
Fragments arranged by size:
4979 439 316 240 186 175 168 161
150 140 100 38 33 28 24 23
1 1 1 1
EcoRI G'AATT_C
Cuts at: 4513 4891 4513
Size: 378 6826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 3472 3944 2728
Size: 744 472 5988
Fragments arranged by size:
5988 744 472
FspI TGC'GCA
Cuts at: 5710 5710
Size: 7204
HaeII r_GCGC'y
Cuts at: 1678 2211 3136 6351 1678
Size: 533 925 3215 2531
Fragments arranged by size:
3215 2531 925 533
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4481 4513 5845 5877 5919
Size: 32 135 32 2909 32 1332 32 42
Cuts at: 5919 5951 1373
Size: 32 2626
Fragments arranged by size:
2909 2626 1332 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 3568 4548 4794 669
Size: 988 281 487 1143 980 246 3079
Fragments arranged by size:
3079 1143 988 980 487 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7204
HpaI GTT'AAC
Cuts at: 4794 4794
Size: 7204
KasI G'GCGC_C
Cuts at: 2207 3132 2207
Size: 925 6279
KpnI G_GTAC'C
Cuts at: 4529 4529
Size: 7204
MfeI C'AATT_G
Cuts at: 4803 4803
Size: 7204
MluI A'CGCG_T
Cuts at: 7080 7080
Size: 7204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 2985 3771 4346 4506 6318 6502 1305
Size: 1461 219 786 575 160 1812 184 2007
Fragments arranged by size:
2007 1812 1461 786 575 219 184 160
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 3597 10
Size: 54 1425 343 288 1477 3617
Fragments arranged by size:
3617 1477 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3701 3704 5162 5521 5680 518
Size: 648 2535 3 1458 359 159 2042
Fragments arranged by size:
2535 2042 1458 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2893 3019 3199 3400 3478 3487 3579 3765
Size: 1287 126 180 201 78 9 92 186
Cuts at: 3765 4620 5297 6445 6690 1606
Size: 855 677 1148 245 2120
Fragments arranged by size:
2120 1287 1148 855 677 245 201 186
180 126 92 78 9
NaeI GCC'GGC
Cuts at: 3463 4067 3463
Size: 604 6600
NarI GG'CG_CC
Cuts at: 2208 3133 2208
Size: 925 6279
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5713
Fragments arranged by size:
5713 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7204
NgoMIV G'CCGG_C
Cuts at: 3461 4065 3461
Size: 604 6600
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7204
NotI GC'GGCC_GC
Cuts at: 4651 4651
Size: 7204
NspI r_CATG'y
Cuts at: 1132 1229 2431 2859 2977 3626 4253 4562 6107
Size: 97 1202 428 118 649 627 309 1545
Cuts at: 6107 1132
Size: 2229
Fragments arranged by size:
2229 1545 1202 649 627 428 309 118
97
PasI CC'CwG_GG
Cuts at: 1348 3372 4018 1348
Size: 2024 646 4534
Fragments arranged by size:
4534 2024 646
PciI A'CATG_T
Cuts at: 2973 6103 2973
Size: 3130 4074
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3274 3838 1108
Size: 2166 564 4474
Fragments arranged by size:
4474 2166 564
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 7204
PmeI GTTT'AAAC
Cuts at: 4508 4508
Size: 7204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5273 5305 6786 6818 5273
Size: 32 1481 32 5659
Fragments arranged by size:
5659 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3544 3784 3807 4113 3544
Size: 240 23 306 6635
Fragments arranged by size:
6635 306 240 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7204
PsiI TTA'TAA
Cuts at: 4774 4774
Size: 7204
PspOMI G'GGCC_C
Cuts at: 2591 3031 3368 3972 4274 2591
Size: 440 337 604 302 5521
Fragments arranged by size:
5521 604 440 337 302
PstI C_TGCA'G
Cuts at: 830 2358 2820 2898 2916 3399 4458 4556 830
Size: 1528 462 78 18 483 1059 98 3478
Fragments arranged by size:
3478 1528 1059 483 462 98 78 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7204
PvuII CAG'CTG
Cuts at: 2893 3400 3478 3487 2893
Size: 507 78 9 6610
Fragments arranged by size:
6610 507 78 9
SacI G_AGCT'C
Cuts at: 4431 4431
Size: 7204
SalI G'TCGA_C
Cuts at: 1936 4546 1936
Size: 2610 4594
SanDI GG'GwC_CC
Cuts at: 3544 3544
Size: 7204
SapI GCTCTTCn'nnn_
Cuts at: 4074 4074
Size: 7204
SbfI CC_TGCA'GG
Cuts at: 2916 4556 2916
Size: 1640 5564
ScaI AGT'ACT
Cuts at: 5452 7164 5452
Size: 1712 5492
SexAI A'CCwGG_T
Cuts at: 3270 3720 3270
Size: 450 6754
SfcI C'TryA_G
Cuts at: 826 1045 2354 2816 2894 2912 3395 4454 4552
Size: 219 1309 462 78 18 483 1059 98
Cuts at: 4552 5687 6368 6559 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1309 1135 1059 681 483 462 219
191 98 78 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2508 2508
Size: 7204
SfoI GGC'GCC
Cuts at: 2209 3134 2209
Size: 925 6279
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7204
SgrAI Cr'CCGG_yG
Cuts at: 1700 4065 1700
Size: 2365 4839
SmaI CCC'GGG
Cuts at: 1872 3542 3805 3971 4531 1872
Size: 1670 263 166 560 4545
Fragments arranged by size:
4545 1670 560 263 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3350 4232 5308 6209 6471 820
Size: 197 926 1407 882 1076 901 262 1553
Fragments arranged by size:
1553 1407 1076 926 901 882 262 197
SnaBI TAC'GTA
Cuts at: 493 4995 493
Size: 4502 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7204
SphI G_CATG'C
Cuts at: 1132 2859 4253 4562 1132
Size: 1727 1394 309 3774
Fragments arranged by size:
3774 1727 1394 309
SrfI GCCC'GGGC
Cuts at: 3971 3971
Size: 7204
SspI AAT'ATT
Cuts at: 5 52 5128 5
Size: 47 5076 2081
Fragments arranged by size:
5076 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7204
StyI C'CwwG_G
Cuts at: 513 1065 2004 3960 4098 4116 4140 4643 513
Size: 552 939 1956 138 18 24 503 3074
Fragments arranged by size:
3074 1956 939 552 503 138 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 3281 3581 5231 5248 5401 5586
Size: 1255 762 939 300 1650 17 153 185
Cuts at: 5586 6031 325
Size: 445 1498
Fragments arranged by size:
1650 1498 1255 939 762 445 300 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 5450 7162
Size: 274 80 33 51 1443 555 2918 1712
Cuts at: 7162 96
Size: 138
Fragments arranged by size:
2918 1712 1443 555 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3728 3760 7164 7196 3728
Size: 32 3404 32 3736
Fragments arranged by size:
3736 3404 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2101 2705 3530 1316
Size: 785 604 825 4990
Fragments arranged by size:
4990 825 785 604
XbaI T'CTAG_A
Cuts at: 4540 4540
Size: 7204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 3580 3424
Size: 156 7048
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7204
XmaI C'CCGG_G
Cuts at: 1870 3540 3803 3969 4529 1870
Size: 1670 263 166 560 4545
Fragments arranged by size:
4545 1670 560 263 166
XmnI GAAnn'nnTTC
Cuts at: 5333 5333
Size: 7204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5012 276
Size: 53 83 186 936 3478 2468
Fragments arranged by size:
3478 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SwaI