Restriction map for
pFN21AA1732
(Circular) (Six-base) MAPSORT of: pFN21AA1732.seq Check: 2281 from: 1 to: 12631
pFN21AA1732 12631 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 8401 2599
Size: 5802 6829
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10441 278
Size: 53 83 186 936 8905 2468
Fragments arranged by size:
8905 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 2553 5099 5689 5938 9974 1937
Size: 397 219 2546 590 249 4036 4594
Fragments arranged by size:
4594 4036 2546 590 397 249 219
Acc65I G'GTAC_C
Cuts at: 9952 9952
Size: 12631
AclI AA'CG_TT
Cuts at: 1563 7226 10758 11131 1563
Size: 5663 3532 373 3063
Fragments arranged by size:
5663 3532 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2218 2312 2967 3552 4431 4446 4637
Size: 462 124 94 655 585 879 15 191
Cuts at: 4637 6068 6251 6349 6851 7194 7559 7610 8362
Size: 1431 183 98 502 343 365 51 752
Cuts at: 8362 9206 9765 9821 10694 12078 12346 1632
Size: 844 559 56 873 1384 268 1917
Fragments arranged by size:
1917 1431 1384 879 873 844 752 655
585 559 502 462 365 343 268 191
183 124 98 94 56 51 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12434
AflIII A'CryG_T
Cuts at: 1203 2800 4969 5737 5891 8920 9140 11530 12507
Size: 1597 2169 768 154 3029 220 2390 977
Cuts at: 12507 1203
Size: 1327
Fragments arranged by size:
3029 2390 2169 1597 1327 977 768 220
154
AgeI A'CCGG_T
Cuts at: 11462 11462
Size: 12631
AhdI GACnn_n'nnGTC
Cuts at: 6097 11360 6097
Size: 5263 7368
AleI CACnn'nnGTG
Cuts at: 1166 7128 1166
Size: 5962 6669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4078 1184
Size: 2894 9737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3603 3635 3603
Size: 32 12599
AlwNI CAG_nnn'CTG
Cuts at: 2972 4046 4592 7841 8105 8668 8933 9694 10374
Size: 1074 546 3249 264 563 265 761 680
Cuts at: 10374 11946 2972
Size: 1572 3657
Fragments arranged by size:
3657 3249 1572 1074 761 680 563 546
265 264
ApaI G_GGCC'C
Cuts at: 6345 6345
Size: 12631
ApaLI G'TGCA_C
Cuts at: 10688 11844 10688
Size: 1156 11475
ApoI r'AATT_y
Cuts at: 1493 2427 2877 3023 3707 3715 3920 4522 4541
Size: 934 450 146 684 8 205 602 19
Cuts at: 4541 4903 5020 5227 5351 5430 5557 6068 8310
Size: 362 117 207 124 79 127 511 2242
Cuts at: 8310 9940 10166 10318 12427 1493
Size: 1630 226 152 2109 1697
Fragments arranged by size:
2242 2109 1697 1630 934 684 602 511
450 362 226 207 205 152 146 127
124 117 79 19 8
AseI AT'TA_AT
Cuts at: 160 1032 3813 8160 11185 160
Size: 872 2781 4347 3025 1606
Fragments arranged by size:
4347 3025 2781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12631
AvaI C'yCGr_G
Cuts at: 1870 1943 6777 9432 9956 1870
Size: 73 4834 2655 524 4545
Fragments arranged by size:
4834 4545 2655 524 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4335 4368 5843 5876 4335
Size: 33 1475 33 11090
Fragments arranged by size:
11090 1475 33 33
BamHI G'GATC_C
Cuts at: 5923 6041 8770 9490 9961 10328 5923
Size: 118 2729 720 471 367 8226
Fragments arranged by size:
8226 2729 720 471 367 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 6966 8914 9952 11407
Size: 325 217 627 390 4789 1948 1038 1455
Cuts at: 11407 618
Size: 1842
Fragments arranged by size:
4789 1948 1842 1455 1038 627 390 325
217
BanII G_rGCy'C
Cuts at: 1391 3068 3317 5769 6345 6535 8124 9950 1391
Size: 1677 249 2452 576 190 1589 1826 4072
Fragments arranged by size:
4072 2452 1826 1677 1589 576 249 190
BbeI G_GCGC'C
Cuts at: 6970 6970
Size: 12631
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3410 4652 4936 5040 6566 8408 9212
Size: 971 1511 1242 284 104 1526 1842 804
Cuts at: 9212 9431 9753 928
Size: 219 322 3806
Fragments arranged by size:
3806 1842 1526 1511 1242 971 804 322
284 219 104
BbvCI CC'TCA_GC
Cuts at: 6938 9321 6938
Size: 2383 10248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10822 10856 1903
Size: 34 8885 34 3678
Fragments arranged by size:
8885 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10523 11733 10523
Size: 1210 11421
BclI T'GATC_A
Cuts at: 1548 3985 7966 8848 9468 1548
Size: 2437 3981 882 620 4711
Fragments arranged by size:
4711 3981 2437 882 620
BfrBI ATG'CAT
Cuts at: 4146 5057 4146
Size: 911 11720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11242 136
Size: 107 122 71 631 605 9570 1525
Fragments arranged by size:
9570 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2711 3215 6886 12626 2711
Size: 504 3671 5740 2716
Fragments arranged by size:
5740 3671 2716 504
BlpI GC'TnA_GC
Cuts at: 3067 7445 7829 8856 10048 3067
Size: 4378 384 1027 1192 5650
Fragments arranged by size:
5650 4378 1192 1027 384
Bme1580I G_kGCm'C
Cuts at: 1790 6321 6345 10692 11848 1790
Size: 4531 24 4347 1156 2573
Fragments arranged by size:
4531 4347 2573 1156 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12631
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3623 5702 5929
Size: 416 53 83 399 24 2206 2079 227
Cuts at: 5929 5961 8819 8947 9023 9670 10362 11320 442
Size: 32 2858 128 76 647 692 958 1753
Fragments arranged by size:
2858 2206 2079 1753 958 692 647 416
399 227 128 83 76 53 32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12631
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3234 3256 6770 6871 7441 3234
Size: 22 3514 101 570 8424
Fragments arranged by size:
8424 3514 570 101 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 3831 4704 5186 6891 8977 9610 9848
Size: 1059 1533 873 482 1705 2086 633 238
Cuts at: 9848 11291 1239
Size: 1443 2579
Fragments arranged by size:
2579 2086 1705 1533 1443 1059 873 633
482 238
Bpu10I CC'TnA_GC
Cuts at: 6938 9321 6938
Size: 2383 10248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4875 5778 5958 6519 6715 9096 9551 10756
Size: 1478 903 180 561 196 2381 455 1205
Cuts at: 10756 11621 11919 3397
Size: 865 298 4109
Fragments arranged by size:
4109 2381 1478 1205 903 865 561 455
298 196 180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3441 3491 3849 7044 7427 7725 8421
Size: 617 1942 50 358 3195 383 298 696
Cuts at: 8421 11294 882
Size: 2873 2219
Fragments arranged by size:
3195 2873 2219 1942 696 617 383 358
298 50
BsaAI yAC'GTr
Cuts at: 493 1204 2803 5814 5894 10422 493
Size: 711 1599 3011 80 4528 2702
Fragments arranged by size:
4528 3011 2702 1599 711 80
BsaBI GATnn'nnATC
Cuts at: 3187 4614 7965 12625 3187
Size: 1427 3351 4660 3193
Fragments arranged by size:
4660 3351 3193 1427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6967 10438 10820 11455
Size: 53 83 186 936 5434 3471 382 635
Cuts at: 11455 275
Size: 1451
Fragments arranged by size:
5434 3471 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5425 9705 11064 11462 11736 11883 12294 5425
Size: 4280 1359 398 274 147 411 5762
Fragments arranged by size:
5762 4280 1359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4621 4651 4956 4986 7837
Size: 30 165 30 3218 30 305 30 2851
Cuts at: 7837 7867 8007 8037 8183 8213 8975 9005 9258
Size: 30 140 30 146 30 762 30 253
Cuts at: 9258 9288 9788 9818 1178
Size: 30 500 30 3991
Fragments arranged by size:
3991 3218 2851 762 500 305 253 165
146 140 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4971 6032 7323 8204 9375 1186
Size: 3785 1061 1291 881 1171 4442
Fragments arranged by size:
4442 3785 1291 1171 1061 881
BseYI C'CCAG_C
Cuts at: 3839 6687 6954 8774 8813 9210 11834 12330 3839
Size: 2848 267 1820 39 397 2624 496 4140
Fragments arranged by size:
4140 2848 2624 1820 496 397 267 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2259 6896 9087 1163
Size: 573 393 130 4637 2191 4707
Fragments arranged by size:
4707 4637 2191 573 393 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7606 10081 10842 11870 1540
Size: 275 186 5605 2475 761 1028 2301
Fragments arranged by size:
5605 2475 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3068 3317 3370 5769 6535 7446 7792 7835
Size: 1004 249 53 2399 766 911 346 43
Cuts at: 7835 8802 10692 10777 11848 2064
Size: 967 1890 85 1071 2847
Fragments arranged by size:
2847 2399 1890 1071 1004 967 911 766
346 249 85 53 43
BsmI GAATG_Cn'
Cuts at: 6498 8048 10142 10235 6498
Size: 1550 2094 93 8894
Fragments arranged by size:
8894 2094 1550 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5204 6836 7002 5204
Size: 1632 166 10833
Fragments arranged by size:
10833 1632 166
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 3068 3317 3370 5769 6321 6345
Size: 399 274 1004 249 53 2399 552 24
Cuts at: 6345 6535 7446 7792 7835 8124 8802 9950 10692
Size: 190 911 346 43 289 678 1148 742
Cuts at: 10692 10777 11848 1391
Size: 85 1071 2174
Fragments arranged by size:
2399 2174 1148 1071 1004 911 742 678
552 399 346 289 274 249 190 85
53 43 24
BspEI T'CCGG_A
Cuts at: 5425 12294 5425
Size: 6869 5762
BspHI T'CATG_A
Cuts at: 5107 6579 7116 10520 12250 5107
Size: 1472 537 3404 1730 5488
Fragments arranged by size:
5488 3404 1730 1472 537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 4217 8401 9986 844
Size: 1755 1618 4184 1585 3489
Fragments arranged by size:
4184 3489 1755 1618 1585
BsrBI CCG'CTC
Cuts at: 1394 1823 8506 10518 1394
Size: 429 6683 2012 3507
Fragments arranged by size:
6683 3507 2012 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 5917 9841 11126 11300 65
Size: 1183 1042 3627 3924 1285 174 1396
Fragments arranged by size:
3924 3627 1396 1285 1183 1042 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9016 11275 11462 1700
Size: 110 7206 2259 187 2869
Fragments arranged by size:
7206 2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 3728 6506 9082 9196 96
Size: 3632 2778 2576 114 3531
Fragments arranged by size:
3632 3531 2778 2576 114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12631
BssSI C'ACGA_G
Cuts at: 3369 10691 11703 3369
Size: 7322 1012 4297
Fragments arranged by size:
7322 4297 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6907 8659 794
Size: 6113 1752 4766
Fragments arranged by size:
6113 4766 1752
BstBI TT'CG_AA
Cuts at: 6719 11435 6719
Size: 4716 7915
BstEII G'GTnAC_C
Cuts at: 1184 5549 7298 8735 1184
Size: 4365 1749 1437 5080
Fragments arranged by size:
5080 4365 1749 1437
BstXI CCAn_nnnn'nTGG
Cuts at: 6381 7201 7250 8275 8972 6381
Size: 820 49 1025 697 10040
Fragments arranged by size:
10040 1025 820 697 49
BstYI r'GATC_y
Cuts at: 1972 2711 3088 3215 4028 5923 6041 6886 7578
Size: 739 377 127 813 1895 118 845 692
Cuts at: 7578 7960 8770 9367 9436 9490 9961 10328 10713
Size: 382 810 597 69 54 471 367 385
Cuts at: 10713 10730 12182 12268 12280 12626 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1895 1452 845 813 810 739 692
597 471 385 382 377 367 346 127
118 86 69 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 3452 9819 3452
Size: 6367 6264
BtgI C'CryG_G
Cuts at: 513 1065 5028 5241 513
Size: 552 3963 213 7903
Fragments arranged by size:
7903 3963 552 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6394 7767 487
Size: 683 56 5168 1373 5351
Fragments arranged by size:
5351 5168 1373 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 2468 2535 4740 6503 6884 6896 8684 9297
Size: 197 67 2205 1763 381 12 1788 613
Cuts at: 9297 10147 10941 10961 2271
Size: 850 794 20 3941
Fragments arranged by size:
3941 2205 1788 1763 850 794 613 381
197 67 20 12
ClaI AT'CG_AT
Cuts at: 1554 3862 1554
Size: 2308 10323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12596
DraI TTT'AAA
Cuts at: 3021 3058 3078 3712 3885 4767 6772 9754 9935
Size: 37 20 634 173 882 2005 2982 181
Cuts at: 9935 3021
Size: 5717
Fragments arranged by size:
5717 2982 2005 882 634 181 173 37
20
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9141 11638 809
Size: 507 7825 2497 1802
Fragments arranged by size:
7825 2497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3347 7028 7603
Size: 54 1425 50 275 18 1517 3681 575
Cuts at: 7603 8262 10078 10967 12596 8
Size: 659 1816 889 1629 43
Fragments arranged by size:
3681 1816 1629 1517 1425 889 659 575
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7603 10078 1537
Size: 275 5791 2475 4090
Fragments arranged by size:
5791 4090 2475 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 3387 3442 3517 4656 4918 5131 5598
Size: 978 1056 55 75 1139 262 213 467
Cuts at: 5598 5757 6009 7626 7989 8181 8497 10561 1353
Size: 159 252 1617 363 192 316 2064 3423
Fragments arranged by size:
3423 2064 1617 1139 1056 978 467 363
316 262 252 213 192 159 75 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2562 3223 11218 11592 11738 387
Size: 1064 138 973 661 7995 374 146 1280
Fragments arranged by size:
7995 1280 1064 973 661 374 146 138
EcoICRI GAG'CTC
Cuts at: 3066 3315 5767 6533 3066
Size: 249 2452 766 9164
Fragments arranged by size:
9164 2452 766 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2218 2298 2312 2967 3552 3831
Size: 393 462 124 80 14 655 585 279
Cuts at: 3831 4431 4446 4637 4704 5186 6068 6251 6349
Size: 600 15 191 67 482 882 183 98
Cuts at: 6349 6851 6891 7194 7559 7610 8362 8977 9206
Size: 502 40 303 365 51 752 615 229
Cuts at: 9206 9610 9765 9821 9848 10694 11291 12078 12346
Size: 404 155 56 27 846 597 787 268
Cuts at: 12346 1239
Size: 1524
Fragments arranged by size:
1524 882 846 787 752 655 615 600
597 585 502 482 462 404 393 365
303 279 268 229 191 183 155 124
98 80 67 56 51 40 27 15
14
EcoNI CCTnn'n_nnAGG
Cuts at: 3262 4818 8297 3262
Size: 1556 3479 7596
Fragments arranged by size:
7596 3479 1556
EcoO109I rG'GnC_Cy
Cuts at: 6209 6342 6370 9823 6209
Size: 133 28 3453 9017
Fragments arranged by size:
9017 3453 133 28
EcoRI G'AATT_C
Cuts at: 4903 5227 8310 9940 10318 4903
Size: 324 3083 1630 378 7216
Fragments arranged by size:
7216 3083 1630 378 324
EcoRV GAT'ATC
Cuts at: 6196 6196
Size: 12631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6581 7979 6581
Size: 1398 11233
FspI TGC'GCA
Cuts at: 11137 11137
Size: 12631
HaeII r_GCGC'y
Cuts at: 1678 6970 11778 1678
Size: 5292 4808 2531
Fragments arranged by size:
5292 4808 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 2765 2797 2822
Size: 32 135 32 788 32 373 32 25
Cuts at: 2822 2854 3953 3985 4700 4732 4923 4955 5519
Size: 32 1099 32 715 32 191 32 564
Cuts at: 5519 5551 5915 5947 6083 6115 6833 6835 6865
Size: 32 364 32 136 32 718 2 30
Cuts at: 6865 6867 6878 6910 7311 7343 7766 7798 8183
Size: 2 11 32 401 32 423 32 385
Cuts at: 8183 8215 9343 9375 11272 11304 11346 11378 1373
Size: 32 1128 32 1897 32 42 32 2626
Fragments arranged by size:
2626 1897 1128 1099 788 718 715 564
423 401 385 373 364 191 136 135
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 30 25 11 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 7224 9975 10221 669
Size: 988 281 5286 2751 246 3079
Fragments arranged by size:
5286 3079 2751 988 281 246
HindIII A'AGCT_T
Cuts at: 748 7622 748
Size: 6874 5757
HpaI GTT'AAC
Cuts at: 7224 10221 7224
Size: 2997 9634
KasI G'GCGC_C
Cuts at: 6966 6966
Size: 12631
KpnI G_GTAC'C
Cuts at: 9956 9956
Size: 12631
MfeI C'AATT_G
Cuts at: 7288 9192 10230 7288
Size: 1904 1038 9689
Fragments arranged by size:
9689 1904 1038
MluI A'CGCG_T
Cuts at: 12507 12507
Size: 12631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3646 7662 8264 9170 9587 11745 11929 1305
Size: 2341 4016 602 906 417 2158 184 2007
Fragments arranged by size:
4016 2341 2158 2007 906 602 417 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 7030 8264 10
Size: 54 1425 343 5198 1234 4377
Fragments arranged by size:
5198 4377 1425 1234 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3285 4289 4605 5062 5340 5526 5742
Size: 648 2119 1004 316 457 278 186 216
Cuts at: 5742 6379 6654 7128 10589 10948 11107 518
Size: 637 275 474 3461 359 159 2042
Fragments arranged by size:
3461 2119 2042 1004 648 637 474 457
359 316 278 275 216 186 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2318 7128 8035 10047 10724 11872 12117
Size: 408 304 4810 907 2012 677 1148 245
Cuts at: 12117 1606
Size: 2120
Fragments arranged by size:
4810 2120 2012 1148 907 677 408 304
245
NaeI GCC'GGC
Cuts at: 9018 9018
Size: 12631
NarI GG'CG_CC
Cuts at: 6967 6967
Size: 12631
NcoI C'CATG_G
Cuts at: 513 1065 5028 5241 513
Size: 552 3963 213 7903
Fragments arranged by size:
7903 3963 552 213
NdeI CA'TA_TG
Cuts at: 387 9568 387
Size: 9181 3450
NgoMIV G'CCGG_C
Cuts at: 9016 9016
Size: 12631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12631
NotI GC'GGCC_GC
Cuts at: 10078 10078
Size: 12631
NsiI A_TGCA'T
Cuts at: 4148 5059 4148
Size: 911 11720
NspI r_CATG'y
Cuts at: 1132 1229 4146 4150 4216 4551 4775 4973 5741
Size: 97 2917 4 66 335 224 198 768
Cuts at: 5741 6552 6653 8924 9144 9883 9989 11534 1132
Size: 811 101 2271 220 739 106 1545 2229
Fragments arranged by size:
2917 2271 2229 1545 811 768 739 335
224 220 198 106 101 97 66 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12631
PciI A'CATG_T
Cuts at: 4969 5737 8920 9140 11530 4969
Size: 768 3183 220 2390 6070
Fragments arranged by size:
6070 3183 2390 768 220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 8959 1108
Size: 7851 4780
PfoI T'CCnGG_A
Cuts at: 5944 8274 5944
Size: 2330 10301
PmeI GTTT'AAAC
Cuts at: 9935 9935
Size: 12631
PmlI CAC'GTG
Cuts at: 5894 5894
Size: 12631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7508 7540 10700 10732 12213 12245 7508
Size: 32 3160 32 1481 32 7894
Fragments arranged by size:
7894 3160 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6370 9823 6370
Size: 3453 9178
PshAI GACnn'nnGTC
Cuts at: 1613 3628 7736 1613
Size: 2015 4108 6508
Fragments arranged by size:
6508 4108 2015
PsiI TTA'TAA
Cuts at: 2735 3669 3926 7640 7889 8709 10201 2735
Size: 934 257 3714 249 820 1492 5165
Fragments arranged by size:
5165 3714 1492 934 820 257 249
PspOMI G'GGCC_C
Cuts at: 6341 6341
Size: 12631
PspXI vC'TCGA_Gb
Cuts at: 6777 6777
Size: 12631
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4567 4599 4738 4770 4567
Size: 32 139 32 12428
Fragments arranged by size:
12428 139 32 32
PstI C_TGCA'G
Cuts at: 830 2019 2386 9983 830
Size: 1189 367 7597 3478
Fragments arranged by size:
7597 3478 1189 367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12631
PvuII CAG'CTG
Cuts at: 2014 2318 7128 8035 2014
Size: 304 4810 907 6610
Fragments arranged by size:
6610 4810 907 304
SacI G_AGCT'C
Cuts at: 3068 3317 5769 6535 3068
Size: 249 2452 766 9164
Fragments arranged by size:
9164 2452 766 249
SalI G'TCGA_C
Cuts at: 1936 9973 1936
Size: 8037 4594
SanDI GG'GwC_CC
Cuts at: 6370 6370
Size: 12631
SapI GCTCTTCn'nnn_
Cuts at: 5131 5598 5757 5131
Size: 467 159 12005
Fragments arranged by size:
12005 467 159
SbfI CC_TGCA'GG
Cuts at: 9983 9983
Size: 12631
ScaI AGT'ACT
Cuts at: 7698 9095 9806 10879 12591 7698
Size: 1397 711 1073 1712 7738
Fragments arranged by size:
7738 1712 1397 1073 711
SexAI A'CCwGG_T
Cuts at: 7437 8743 7437
Size: 1306 11325
SfcI C'TryA_G
Cuts at: 826 1045 2015 2382 2550 5100 7986 8589 8877
Size: 219 970 367 168 2550 2886 603 288
Cuts at: 8877 9979 11114 11795 11986 826
Size: 1102 1135 681 191 1471
Fragments arranged by size:
2886 2550 1471 1135 1102 970 681 603
367 288 219 191 168
SfoI GGC'GCC
Cuts at: 6968 6968
Size: 12631
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12631
SmaI CCC'GGG
Cuts at: 1872 9958 1872
Size: 8086 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3412 4890 5793 5973 6534 6694
Size: 197 926 1469 1478 903 180 561 160
Cuts at: 6694 6777 9111 9432 9530 10735 11636 11898 820
Size: 83 2334 321 98 1205 901 262 1553
Fragments arranged by size:
2334 1553 1478 1469 1205 926 903 901
561 321 262 197 180 160 98 83
SnaBI TAC'GTA
Cuts at: 493 10422 493
Size: 9929 2702
SpeI A'CTAG_T
Cuts at: 152 4580 152
Size: 4428 8203
SphI G_CATG'C
Cuts at: 1132 4146 4150 4216 4775 6653 9989 1132
Size: 3014 4 66 559 1878 3336 3774
Fragments arranged by size:
3774 3336 3014 1878 559 66 4
SspI AAT'ATT
Cuts at: 5 52 2814 3134 4174 4438 6740 10555 5
Size: 47 2762 320 1040 264 2302 3815 2081
Fragments arranged by size:
3815 2762 2302 2081 1040 320 264 47
StuI AGG'CCT
Cuts at: 1517 8762 9590 1517
Size: 7245 828 4558
Fragments arranged by size:
7245 4558 828
StyI C'CwwG_G
Cuts at: 513 1065 5028 5241 8862 9858 10070 513
Size: 552 3963 213 3621 996 212 3074
Fragments arranged by size:
3963 3621 3074 996 552 213 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5568 8827 10658 10675 10828 11013 11458
Size: 1255 3988 3259 1831 17 153 185 445
Cuts at: 11458 325
Size: 1498
Fragments arranged by size:
3988 3259 1831 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 4388 5513
Size: 274 80 33 51 1443 1751 660 1125
Cuts at: 5513 6506 7696 8980 9082 9093 9196 9804 9864
Size: 993 1190 1284 102 11 103 608 60
Cuts at: 9864 9876 10877 12589 96
Size: 12 1001 1712 138
Fragments arranged by size:
1751 1712 1443 1284 1190 1125 1001 993
660 608 274 138 103 102 80 60
51 33 12 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4662 4694 4956 4988 6376 6408 7514 7546 7798
Size: 32 262 32 1388 32 1106 32 252
Cuts at: 7798 7830 7837 7869 8813 8845 8975 9007 9241
Size: 32 7 32 944 32 130 32 234
Cuts at: 9241 9273 9815 9847 12591 12623 4662
Size: 32 542 32 2744 32 4670
Fragments arranged by size:
4670 2744 1388 1106 944 542 262 252
234 130 32 32 32 32 32 32
32 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 1316 6847 1316
Size: 5531 7100
XbaI T'CTAG_A
Cuts at: 3242 9967 3242
Size: 6725 5906
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 5649 8272 8815 2470
Size: 3179 2623 543 6286
Fragments arranged by size:
6286 3179 2623 543
XhoI C'TCGA_G
Cuts at: 1943 6777 9432 1943
Size: 4834 2655 5142
Fragments arranged by size:
5142 4834 2655
XmaI C'CCGG_G
Cuts at: 1870 9956 1870
Size: 8086 4545
XmnI GAAnn'nnTTC
Cuts at: 2877 7271 10760 2877
Size: 4394 3489 4748
Fragments arranged by size:
4748 4394 3489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10439 276
Size: 53 83 186 936 8905 2468
Fragments arranged by size:
8905 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI BstZ17I DraIII FseI FspAI
NruI PacI RsrII SacII SfiI SrfI SwaI