Restriction map for
pFN21AA1726
(Circular) (Six-base) MAPSORT of: pFN21AA1726.seq Check: 3284 from: 1 to: 7357
pFN21AA1726 7357 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4143 4143
Size: 7357
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5167 278
Size: 53 83 186 936 3631 2468
Fragments arranged by size:
3631 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3504 3762 4700 1937
Size: 1567 258 938 4594
Fragments arranged by size:
4594 1567 938 258
Acc65I G'GTAC_C
Cuts at: 4371 4678 4371
Size: 307 7050
AclI AA'CG_TT
Cuts at: 1563 5484 5857 1563
Size: 3921 373 3063
Fragments arranged by size:
3921 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 2298 2454 2553 5420 6804 7072 1632
Size: 639 27 156 99 2867 1384 268 1917
Fragments arranged by size:
2867 1917 1384 639 268 156 99 27
AflII C'TTAA_G
Cuts at: 820 1017 2516 820
Size: 197 1499 5661
Fragments arranged by size:
5661 1499 197
AflIII A'CryG_T
Cuts at: 1203 2087 6256 7233 1203
Size: 884 4169 977 1327
Fragments arranged by size:
4169 1327 977 884
AgeI A'CCGG_T
Cuts at: 3942 6188 3942
Size: 2246 5111
AhdI GACnn_n'nnGTC
Cuts at: 6086 6086
Size: 7357
AleI CACnn'nnGTG
Cuts at: 1166 4411 1166
Size: 3245 4112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7357
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4080 4112 4080
Size: 32 7325
AlwNI CAG_nnn'CTG
Cuts at: 3338 5100 6672 3338
Size: 1762 1572 4023
Fragments arranged by size:
4023 1762 1572
ApaLI G'TGCA_C
Cuts at: 5414 6570 5414
Size: 1156 6201
ApoI r'AATT_y
Cuts at: 1493 2290 2412 2928 3982 4042 4666 4892 5044
Size: 797 122 516 1054 60 624 226 152
Cuts at: 5044 7153 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1054 797 624 516 226 152
122 60
AseI AT'TA_AT
Cuts at: 160 1032 3131 5911 160
Size: 872 2099 2780 1606
Fragments arranged by size:
2780 2099 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7357
AvaI C'yCGr_G
Cuts at: 1870 1943 4501 4682 1870
Size: 73 2558 181 4545
Fragments arranged by size:
4545 2558 181 73
AvrII C'CTAG_G
Cuts at: 2119 4222 2119
Size: 2103 5254
BamHI G'GATC_C
Cuts at: 4470 4687 5054 4470
Size: 217 367 6773
Fragments arranged by size:
6773 367 217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4154 4213 4371 4678 6133
Size: 325 217 627 2367 59 158 307 1455
Cuts at: 6133 618
Size: 1842
Fragments arranged by size:
2367 1842 1455 627 325 307 217 158
59
BanII G_rGCy'C
Cuts at: 1391 4676 1391
Size: 3285 4072
BbeI G_GCGC'C
Cuts at: 4158 4158
Size: 7357
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5548 5582 1903
Size: 34 3611 34 3678
Fragments arranged by size:
3678 3611 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2206 3750 5249 6459 2206
Size: 1544 1499 1210 3104
Fragments arranged by size:
3104 1544 1499 1210
BclI T'GATC_A
Cuts at: 1548 2296 2632 1548
Size: 748 336 6273
Fragments arranged by size:
6273 748 336
BfrBI ATG'CAT
Cuts at: 3791 3791
Size: 7357
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2977 4323 4465
Size: 107 122 71 631 605 1305 1346 142
Cuts at: 4465 5968 136
Size: 1503 1525
Fragments arranged by size:
1525 1503 1346 1305 631 605 142 122
107 71
BglII A'GATC_T
Cuts at: 7352 7352
Size: 7357
BlpI GC'TnA_GC
Cuts at: 4774 4774
Size: 7357
Bme1580I G_kGCm'C
Cuts at: 1790 5418 6574 1790
Size: 3628 1156 2573
Fragments arranged by size:
3628 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7357
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 5088 6046
Size: 416 53 83 399 24 1772 1899 958
Cuts at: 6046 442
Size: 1753
Fragments arranged by size:
1899 1772 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 3660 6017 1239
Size: 1286 1135 2357 2579
Fragments arranged by size:
2579 2357 1286 1135
Bpu10I CC'TnA_GC
Cuts at: 4446 4446
Size: 7357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 5482 6347 6645 2384
Size: 3098 865 298 3096
Fragments arranged by size:
3098 3096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2379 2737 6020 882
Size: 617 880 358 3283 2219
Fragments arranged by size:
3283 2219 880 617 358
BsaAI yAC'GTr
Cuts at: 493 1204 2090 2936 3928 5148 493
Size: 711 886 846 992 1220 2702
Fragments arranged by size:
2702 1220 992 886 846 711
BsaBI GATnn'nnATC
Cuts at: 4532 7351 4532
Size: 2819 4538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2246 4155 4362 4605
Size: 53 83 186 936 713 1909 207 243
Cuts at: 4605 5164 5546 6181 275
Size: 559 382 635 1451
Fragments arranged by size:
1909 1451 936 713 635 559 382 243
207 186 83 53
BsaWI w'CCGG_w
Cuts at: 3942 5790 6188 6462 6609 7020 3942
Size: 1848 398 274 147 411 4279
Fragments arranged by size:
4279 1848 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2589 2619 3384 3414 1178
Size: 30 165 30 1186 30 765 30 5121
Fragments arranged by size:
5121 1186 765 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3007 3992 4101 4179 1186
Size: 1821 985 109 78 4364
Fragments arranged by size:
4364 1821 985 109 78
BseYI C'CCAG_C
Cuts at: 3580 4335 4608 4644 4648 6560 7056 3580
Size: 755 273 36 4 1912 496 3881
Fragments arranged by size:
3881 1912 755 496 273 36 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4115 1163
Size: 573 2379 4405
Fragments arranged by size:
4405 2379 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4807 5568 6596 1540
Size: 275 186 2806 761 1028 2301
Fragments arranged by size:
2806 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2156 3466 5418 5503 6574 2156
Size: 1310 1952 85 1071 2939
Fragments arranged by size:
2939 1952 1310 1071 85
BsmI GAATG_Cn'
Cuts at: 2028 2046 2263 3793 4491 4868 4961 2028
Size: 18 217 1530 698 377 93 4424
Fragments arranged by size:
4424 1530 698 377 217 93 18
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 3466 4676 5418 5503 6574 1391
Size: 399 366 1310 1210 742 85 1071 2174
Fragments arranged by size:
2174 1310 1210 1071 742 399 366 85
BspEI T'CCGG_A
Cuts at: 7020 7020
Size: 7357
BspHI T'CATG_A
Cuts at: 2299 4578 5246 6976 2299
Size: 2279 668 1730 2680
Fragments arranged by size:
2680 2279 1730 668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 4143 4712 844
Size: 1517 1782 569 3489
Fragments arranged by size:
3489 1782 1517 569
BsrBI CCG'CTC
Cuts at: 1394 1823 5244 1394
Size: 429 3421 3507
Fragments arranged by size:
3507 3421 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2413 2773 2781 3631 5852 6026 65
Size: 1183 1165 360 8 850 2221 174 1396
Fragments arranged by size:
2221 1396 1183 1165 850 360 174 8
BsrFI r'CCGG_y
Cuts at: 1700 1810 3942 4374 6001 6188 1700
Size: 110 2132 432 1627 187 2869
Fragments arranged by size:
2869 2132 1627 432 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7357
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7357
BssSI C'ACGA_G
Cuts at: 4243 5417 6429 4243
Size: 1174 1012 5171
Fragments arranged by size:
5171 1174 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7357
BstBI TT'CG_AA
Cuts at: 6161 6161
Size: 7357
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7357
BstXI CCAn_nnnn'nTGG
Cuts at: 4633 4633
Size: 7357
BstYI r'GATC_y
Cuts at: 1972 3481 4470 4495 4687 5054 5439 5456 6908
Size: 1509 989 25 192 367 385 17 1452
Cuts at: 6908 6994 7006 7352 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1509 1452 989 385 367 346 192
86 25 17 12
Bsu36I CC'TnA_GG
Cuts at: 3657 3657
Size: 7357
BtgI C'CryG_G
Cuts at: 513 1065 2004 2782 513
Size: 552 939 778 5088
Fragments arranged by size:
5088 939 778 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6618
Fragments arranged by size:
6618 683 56
BtsI GCAGTG_nn'
Cuts at: 2832 3895 3922 4873 5667 5687 2832
Size: 1063 27 951 794 20 4502
Fragments arranged by size:
4502 1063 951 794 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7357
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7322
DraI TTT'AAA
Cuts at: 2428 4661 2428
Size: 2233 5124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3758 6364 809
Size: 507 2442 2606 1802
Fragments arranged by size:
2606 2442 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 4239 4804
Size: 54 1425 50 275 18 304 2105 565
Cuts at: 4804 5693 7322 8
Size: 889 1629 43
Fragments arranged by size:
2105 1629 1425 889 565 304 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4804 1537
Size: 275 2992 4090
Fragments arranged by size:
4090 2992 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2247 2430 4119 5287 1353
Size: 659 235 183 1689 1168 3423
Fragments arranged by size:
3423 1689 1168 659 235 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 5944 6318 6464 387
Size: 1064 138 2894 1461 374 146 1280
Fragments arranged by size:
2894 1461 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2298 2454 2525 2553 3660 5420
Size: 393 639 27 156 71 28 1107 1760
Cuts at: 5420 6017 6804 7072 1239
Size: 597 787 268 1524
Fragments arranged by size:
1760 1524 1107 787 639 597 393 268
156 71 28 27
EcoO109I rG'GnC_Cy
Cuts at: 4230 4230
Size: 7357
EcoRI G'AATT_C
Cuts at: 4666 5044 4666
Size: 378 6979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3542 4323 3542
Size: 781 6576
FspI TGC'GCA
Cuts at: 5863 5863
Size: 7357
HaeII r_GCGC'y
Cuts at: 1678 4158 6504 1678
Size: 2480 2346 2531
Fragments arranged by size:
2531 2480 2346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2589 2621 4169
Size: 32 135 32 716 32 269 32 1548
Cuts at: 4169 4201 4244 4276 5998 6030 6072 6104 1373
Size: 32 43 32 1722 32 42 32 2626
Fragments arranged by size:
2626 1722 1548 716 269 135 43 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2190 3763 3995 4701 4947 669
Size: 988 281 252 1573 232 706 246 3079
Fragments arranged by size:
3079 1573 988 706 281 252 246 232
HindIII A'AGCT_T
Cuts at: 748 3524 748
Size: 2776 4581
HpaI GTT'AAC
Cuts at: 4947 4947
Size: 7357
KasI G'GCGC_C
Cuts at: 4154 4154
Size: 7357
KpnI G_GTAC'C
Cuts at: 4375 4682 4375
Size: 307 7050
MfeI C'AATT_G
Cuts at: 2312 4956 2312
Size: 2644 4713
MluI A'CGCG_T
Cuts at: 7233 7233
Size: 7357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 6471 6655 1305
Size: 1719 3447 184 2007
Fragments arranged by size:
3447 2007 1719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4241 10
Size: 54 1425 343 2409 3126
Fragments arranged by size:
3126 2409 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 4411 4631 5315 5674 5833 518
Size: 648 976 2269 220 684 359 159 2042
Fragments arranged by size:
2269 2042 976 684 648 359 220 159
MspA1I CmG'CkG
Cuts at: 1606 2177 3328 4148 4167 4648 4773 5450 6598
Size: 571 1151 820 19 481 125 677 1148
Cuts at: 6598 6843 1606
Size: 245 2120
Fragments arranged by size:
2120 1151 1148 820 677 571 481 245
125 19
NarI GG'CG_CC
Cuts at: 4155 4155
Size: 7357
NcoI C'CATG_G
Cuts at: 513 1065 2004 2782 513
Size: 552 939 778 5088
Fragments arranged by size:
5088 939 778 552
NdeI CA'TA_TG
Cuts at: 387 3259 387
Size: 2872 4485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7357
NotI GC'GGCC_GC
Cuts at: 4804 4804
Size: 7357
NsiI A_TGCA'T
Cuts at: 3793 3793
Size: 7357
NspI r_CATG'y
Cuts at: 1132 1229 4003 4715 6260 1132
Size: 97 2774 712 1545 2229
Fragments arranged by size:
2774 2229 1545 712 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7357
PciI A'CATG_T
Cuts at: 6256 6256
Size: 7357
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4352 4481 1108
Size: 3244 129 3984
Fragments arranged by size:
3984 3244 129
PmeI GTTT'AAAC
Cuts at: 4661 4661
Size: 7357
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5426 5458 6939 6971 5426
Size: 32 1481 32 5812
Fragments arranged by size:
5812 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4230 4230
Size: 7357
PshAI GACnn'nnGTC
Cuts at: 1613 3500 1613
Size: 1887 5470
PsiI TTA'TAA
Cuts at: 4927 4927
Size: 7357
PstI C_TGCA'G
Cuts at: 830 2372 2810 3887 4041 4709 830
Size: 1542 438 1077 154 668 3478
Fragments arranged by size:
3478 1542 1077 668 438 154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7357
PvuII CAG'CTG
Cuts at: 2177 4167 4648 2177
Size: 1990 481 4886
Fragments arranged by size:
4886 1990 481
SalI G'TCGA_C
Cuts at: 1936 3761 4699 1936
Size: 1825 938 4594
Fragments arranged by size:
4594 1825 938
SbfI CC_TGCA'GG
Cuts at: 4709 4709
Size: 7357
ScaI AGT'ACT
Cuts at: 5605 7317 5605
Size: 1712 5645
SexAI A'CCwGG_T
Cuts at: 3551 3551
Size: 7357
SfcI C'TryA_G
Cuts at: 826 1045 2368 2806 3073 3883 4037 4705 5840
Size: 219 1323 438 267 810 154 668 1135
Cuts at: 5840 6521 6712 826
Size: 681 191 1471
Fragments arranged by size:
1471 1323 1135 810 681 668 438 267
219 191 154
SfoI GGC'GCC
Cuts at: 4156 4156
Size: 7357
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7357
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7357
SmaI CCC'GGG
Cuts at: 1872 4684 1872
Size: 2812 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 2516 5461 6362 6624 820
Size: 197 926 456 117 2945 901 262 1553
Fragments arranged by size:
2945 1553 926 901 456 262 197 117
SnaBI TAC'GTA
Cuts at: 493 2936 5148 493
Size: 2443 2212 2702
Fragments arranged by size:
2702 2443 2212
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7357
SphI G_CATG'C
Cuts at: 1132 4715 1132
Size: 3583 3774
SspI AAT'ATT
Cuts at: 5 52 3296 5281 5
Size: 47 3244 1985 2081
Fragments arranged by size:
3244 2081 1985 47
StuI AGG'CCT
Cuts at: 1517 4326 1517
Size: 2809 4548
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2183 2454 2782 3429 4222
Size: 552 939 115 64 271 328 647 793
Cuts at: 4222 4233 4796 513
Size: 11 563 3074
Fragments arranged by size:
3074 939 793 647 563 552 328 271
115 64 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5384 5401 5554 5739 6184 325
Size: 1255 3804 17 153 185 445 1498
Fragments arranged by size:
3804 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2084 2551 2645
Size: 274 80 33 51 1443 107 467 94
Cuts at: 2645 5603 7315 96
Size: 2958 1712 138
Fragments arranged by size:
2958 1712 1443 467 274 138 107 94
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3384 3416 7317 7349 3384
Size: 32 3901 32 3392
Fragments arranged by size:
3901 3392 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7357
XbaI T'CTAG_A
Cuts at: 3359 4693 3359
Size: 1334 6023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2181 3541 2181
Size: 1360 5997
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7357
XmaI C'CCGG_G
Cuts at: 1870 4682 1870
Size: 2812 4545
XmnI GAAnn'nnTTC
Cuts at: 2541 3110 3525 3865 5486 2541
Size: 569 415 340 1621 4412
Fragments arranged by size:
4412 1621 569 415 340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5165 276
Size: 53 83 186 936 3631 2468
Fragments arranged by size:
3631 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbvCI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SrfI SwaI