Restriction map for
pFN21AA1717
(Circular) (Six-base) MAPSORT of: pFN21AA1717.seq Check: 9360 from: 1 to: 5803
pFN21AA1717 5803 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 2591 3146 1937
Size: 328 326 555 4594
Fragments arranged by size:
4594 555 328 326
Acc65I G'GTAC_C
Cuts at: 3060 3124 3060
Size: 64 5739
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 3066 3095 3866 5250 5518 1632
Size: 918 516 29 771 1384 268 1917
Fragments arranged by size:
1917 1384 918 771 516 268 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 5771
AlwNI CAG_nnn'CTG
Cuts at: 2781 3546 5118 2781
Size: 765 1572 3466
Fragments arranged by size:
3466 1572 765
ApaI G_GGCC'C
Cuts at: 2788 2949 2788
Size: 161 5642
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 2477 2566 3112 3338 3490 5599 1493
Size: 984 89 546 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 546 226 152 89
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 3128 1870
Size: 73 1086 99 4545
Fragments arranged by size:
4545 1086 99 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 3060 3124 4579 618
Size: 325 217 627 259 1014 64 1455 1842
Fragments arranged by size:
1842 1455 1014 627 325 259 217 64
BanII G_rGCy'C
Cuts at: 1391 2788 2844 2949 2997 3122 1391
Size: 1397 56 105 48 125 4072
Fragments arranged by size:
4072 1397 125 105 56 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4832
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2866 3695 4905 2866
Size: 829 1210 3764
Fragments arranged by size:
3764 1210 829
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BfrBI ATG'CAT
Cuts at: 2958 2958
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3086 4414 136
Size: 107 122 71 631 605 1414 1328 1525
Fragments arranged by size:
1525 1414 1328 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2258 2788 2949 3864 5020 1790
Size: 259 209 530 161 915 1156 2573
Fragments arranged by size:
2573 1156 915 530 259 209 161
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2791 3534 4492
Size: 416 53 83 399 24 1374 743 958
Cuts at: 4492 442
Size: 1753
Fragments arranged by size:
1753 1374 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2234 2445 2703 4463 1239
Size: 962 33 211 258 1760 2579
Fragments arranged by size:
2579 1760 962 258 211 33
Bpu10I CC'TnA_GC
Cuts at: 2838 2838
Size: 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4466 882
Size: 617 2967 2219
Fragments arranged by size:
2967 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2259 2854 3594 493
Size: 711 1055 595 740 2702
Fragments arranged by size:
2702 1055 740 711 595
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3610 3992 4627 275
Size: 53 83 186 936 2077 382 635 1451
Fragments arranged by size:
2077 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2789 2819 2855 2885 1178
Size: 30 165 30 1386 30 36 30 4096
Fragments arranged by size:
4096 1386 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2086 1186
Size: 859 41 4903
Fragments arranged by size:
4903 859 41
BseYI C'CCAG_C
Cuts at: 3093 5006 5502 3093
Size: 1913 496 3394
Fragments arranged by size:
3394 1913 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 1163
Size: 573 329 4901
Fragments arranged by size:
4901 573 329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2997 3864 3949 5020 2997
Size: 867 85 1071 3780
Fragments arranged by size:
3780 1071 867 85
BsmI GAATG_Cn'
Cuts at: 3314 3407 3314
Size: 93 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2258 2788 2844 2949 2997 3122
Size: 399 259 209 530 56 105 48 125
Cuts at: 3122 3864 3949 5020 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 399 259 209 125
105 85 56 48
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3158 844
Size: 2314 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4447 4634 1700
Size: 110 2637 187 2869
Fragments arranged by size:
2869 2637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5803
BstBI TT'CG_AA
Cuts at: 2749 4607 2749
Size: 1858 3945
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstYI r'GATC_y
Cuts at: 1972 2629 3133 3500 3885 3902 5354 5440 5452
Size: 657 504 367 385 17 1452 86 12
Cuts at: 5452 5798 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 657 504 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4312
Fragments arranged by size:
4312 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5064
Fragments arranged by size:
5064 683 56
BtsI GCAGTG_nn'
Cuts at: 2982 3319 4113 4133 2982
Size: 337 794 20 4652
Fragments arranged by size:
4652 794 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3250 4139 5768
Size: 54 1425 50 275 18 1420 889 1629
Cuts at: 5768 8
Size: 43
Fragments arranged by size:
1629 1425 1420 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 2985 3029 3733 1353
Size: 1272 360 44 704 3423
Fragments arranged by size:
3423 1272 704 360 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2995 2995
Size: 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2234 2445 2550 2703 3066 3095
Size: 393 569 33 211 105 153 363 29
Cuts at: 3095 3866 4463 5250 5518 1239
Size: 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 597 569 393 363 268
211 153 105 33 29
EcoO109I rG'GnC_Cy
Cuts at: 2714 2785 2714
Size: 71 5732
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 5803
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 4950 1678
Size: 3272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373
Size: 32 135 32 2872 32 42 32 2626
Fragments arranged by size:
2872 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2592 2770 3147 3393 669
Size: 988 281 654 178 377 246 3079
Fragments arranged by size:
3079 988 654 377 281 246 178
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3064 3128 3064
Size: 64 5739
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2831 3761 4120 4279 518
Size: 648 1665 930 359 159 2042
Fragments arranged by size:
2042 1665 930 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3219 3896 5044 5289 1606
Size: 1613 677 1148 245 2120
Fragments arranged by size:
2120 1613 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4312
Fragments arranged by size:
4312 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NsiI A_TGCA'T
Cuts at: 2960 2960
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 3161 4706 1132
Size: 97 1932 1545 2229
Fragments arranged by size:
2229 1932 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5803
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 3872 3904 5385 5417 2789
Size: 32 1051 32 1481 32 3175
Fragments arranged by size:
3175 1481 1051 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2714 2714
Size: 5803
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 2739 3373 2739
Size: 634 5169
PspOMI G'GGCC_C
Cuts at: 2784 2945 2784
Size: 161 5642
PstI C_TGCA'G
Cuts at: 830 3155 830
Size: 2325 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
SacI G_AGCT'C
Cuts at: 2997 2997
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 2590 3145 1936
Size: 654 555 4594
Fragments arranged by size:
4594 654 555
SapI GCTCTTCn'nnn_
Cuts at: 2625 2985 2625
Size: 360 5443
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 2864 4051 5763 2864
Size: 1187 1712 2904
Fragments arranged by size:
2904 1712 1187
SexAI A'CCwGG_T
Cuts at: 3063 3063
Size: 5803
SfcI C'TryA_G
Cuts at: 826 1045 3151 4286 4967 5158 826
Size: 219 2106 1135 681 191 1471
Fragments arranged by size:
2106 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3086 3086
Size: 5803
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3031 3130 1872
Size: 1159 99 4545
Fragments arranged by size:
4545 1159 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 2542 3080 1517
Size: 1025 538 4240
Fragments arranged by size:
4240 1025 538
StyI C'CwwG_G
Cuts at: 513 1065 2004 2874 3242 513
Size: 552 939 870 368 3074
Fragments arranged by size:
3074 939 870 552 368
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 3830 3847 4000 4185 4630 325
Size: 1255 1527 723 17 153 185 445 1498
Fragments arranged by size:
1527 1498 1255 723 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2862 4049 5761
Size: 274 80 33 51 1443 885 1187 1712
Cuts at: 5761 96
Size: 138
Fragments arranged by size:
1712 1443 1187 885 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2556 2588 5763 5795 2556
Size: 32 3175 32 2564
Fragments arranged by size:
3175 2564 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2939 2939
Size: 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3029 3128 1870
Size: 1159 99 4545
Fragments arranged by size:
4545 1159 99
XmnI GAAnn'nnTTC
Cuts at: 2156 3080 3932 2156
Size: 924 852 4027
Fragments arranged by size:
4027 924 852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SfoI SrfI SwaI