Restriction map for
pFN21AA1694
(Circular) (Six-base) MAPSORT of: pFN21AA1694.seq Check: 9647 from: 1 to: 7024
pFN21AA1694 7024 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4305 4834 278
Size: 53 83 186 936 2769 529 2468
Fragments arranged by size:
2769 2468 936 529 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4367 1937
Size: 2430 4594
Acc65I G'GTAC_C
Cuts at: 4345 4345
Size: 7024
AclI AA'CG_TT
Cuts at: 1563 5151 5524 1563
Size: 3588 373 3063
Fragments arranged by size:
3588 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3080 3512 3911 4289 5087 6471 6739 1632
Size: 1448 432 399 378 798 1384 268 1917
Fragments arranged by size:
1917 1448 1384 798 432 399 378 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6827
AflIII A'CryG_T
Cuts at: 1203 2082 5923 6900 1203
Size: 879 3841 977 1327
Fragments arranged by size:
3841 1327 977 879
AgeI A'CCGG_T
Cuts at: 5855 5855
Size: 7024
AhdI GACnn_n'nnGTC
Cuts at: 3145 5753 3145
Size: 2608 4416
AleI CACnn'nnGTG
Cuts at: 1166 2798 3842 1166
Size: 1632 1044 4348
Fragments arranged by size:
4348 1632 1044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7024
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4070 4102 4070
Size: 32 6992
AlwNI CAG_nnn'CTG
Cuts at: 2362 3953 4035 4150 4181 4767 6339 2362
Size: 1591 82 115 31 586 1572 3047
Fragments arranged by size:
3047 1591 1572 586 115 82 31
ApaI G_GGCC'C
Cuts at: 3344 3918 3344
Size: 574 6450
ApaLI G'TGCA_C
Cuts at: 3644 5081 6237 3644
Size: 1437 1156 4431
Fragments arranged by size:
4431 1437 1156
ApoI r'AATT_y
Cuts at: 1493 2240 2735 3680 4333 4559 4711 6820 1493
Size: 747 495 945 653 226 152 2109 1697
Fragments arranged by size:
2109 1697 945 747 653 495 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5578 160
Size: 872 4546 1606
Fragments arranged by size:
4546 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2967 4349 1870
Size: 73 80 944 1382 4545
Fragments arranged by size:
4545 1382 944 80 73
BamHI G'GATC_C
Cuts at: 4354 4721 4354
Size: 367 6657
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2202 4345 5800 618
Size: 325 217 627 324 91 2143 1455 1842
Fragments arranged by size:
2143 1842 1455 627 325 324 217 91
BanII G_rGCy'C
Cuts at: 1391 2134 2895 3282 3344 3662 3918 3977 4202
Size: 743 761 387 62 318 256 59 225
Cuts at: 4202 4343 1391
Size: 141 4072
Fragments arranged by size:
4072 761 743 387 318 256 225 141
62 59
BbeI G_GCGC'C
Cuts at: 2115 2115
Size: 7024
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 3525 928
Size: 971 456 1170 4427
Fragments arranged by size:
4427 1170 971 456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5215 5249 1903
Size: 34 3278 34 3678
Fragments arranged by size:
3678 3278 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4916 6126 4916
Size: 1210 5814
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7024
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3245 3922 5635
Size: 107 122 71 631 605 1573 677 1713
Cuts at: 5635 136
Size: 1525
Fragments arranged by size:
1713 1573 1525 677 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2725 4264 7019 2725
Size: 1539 2755 2730
Fragments arranged by size:
2755 2730 1539
BlpI GC'TnA_GC
Cuts at: 3551 3971 4441 3551
Size: 420 470 6134
Fragments arranged by size:
6134 470 420
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2846 2955 3344 3648 3918 5085 6241
Size: 331 725 109 389 304 270 1167 1156
Cuts at: 6241 1790
Size: 2573
Fragments arranged by size:
2573 1167 1156 725 389 331 304 270
109
BmgBI CAC'GTC
Cuts at: 1318 2083 3844 1318
Size: 765 1761 4498
Fragments arranged by size:
4498 1761 765
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2319 4755 5713
Size: 416 53 83 399 24 902 2436 958
Cuts at: 5713 442
Size: 1753
Fragments arranged by size:
2436 1753 958 902 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7024
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3435 3556 3435
Size: 121 6903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3184 3430 3851 4016 4127 5684 1239
Size: 1945 246 421 165 111 1557 2579
Fragments arranged by size:
2579 1945 1557 421 246 165 111
Bpu10I CC'TnA_GC
Cuts at: 4196 4196
Size: 7024
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2801 3164 3368 4052 5149 6014 6312 2801
Size: 363 204 684 1097 865 298 3513
Fragments arranged by size:
3513 1097 865 684 363 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2445 3406 4157 5687 882
Size: 617 552 394 961 751 1530 2219
Fragments arranged by size:
2219 1530 961 751 617 552 394
BsaAI yAC'GTr
Cuts at: 493 1204 3214 3373 3499 4815 493
Size: 711 2010 159 126 1316 2702
Fragments arranged by size:
2702 2010 1316 711 159 126
BsaBI GATnn'nnATC
Cuts at: 7018 7018
Size: 7024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2294 4302 4831
Size: 53 83 186 936 579 182 2008 529
Cuts at: 4831 5213 5848 275
Size: 382 635 1451
Fragments arranged by size:
2008 1451 936 635 579 529 382 186
182 83 53
BsaWI w'CCGG_w
Cuts at: 2550 3069 3096 5457 5855 6129 6276 6687 2550
Size: 519 27 2361 398 274 147 411 2887
Fragments arranged by size:
2887 2361 519 411 398 274 147 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 3749 3779 1178
Size: 30 165 30 912 30 1404 30 4423
Fragments arranged by size:
4423 1404 912 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 1186
Size: 883 6141
BseYI C'CCAG_C
Cuts at: 2071 2253 2526 3131 3337 3709 6227 6723 2071
Size: 182 273 605 206 372 2518 496 2372
Fragments arranged by size:
2518 2372 605 496 372 273 206 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 3110 1163
Size: 573 1089 285 5077
Fragments arranged by size:
5077 1089 573 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3355 4474 5235 6263 1540
Size: 275 186 1096 258 1119 761 1028 2301
Fragments arranged by size:
2301 1119 1096 1028 761 275 258 186
BsiHKAI G_wGCw'C
Cuts at: 2895 3648 3839 3977 4047 4202 5085 5170 6241
Size: 753 191 138 70 155 883 85 1071
Cuts at: 6241 2895
Size: 3678
Fragments arranged by size:
3678 1071 883 753 191 155 138 85
70
BsmI GAATG_Cn'
Cuts at: 2380 4535 4628 2380
Size: 2155 93 4776
Fragments arranged by size:
4776 2155 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2629 3423 4095 2629
Size: 794 672 5558
Fragments arranged by size:
5558 794 672
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2134 2846 2895 2955 3282 3344
Size: 399 331 13 712 49 60 327 62
Cuts at: 3344 3648 3662 3839 3918 3977 4047 4202 4343
Size: 304 14 177 79 59 70 155 141
Cuts at: 4343 5085 5170 6241 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 399 331 327 304
177 155 141 85 79 70 62 60
59 49 14 13
BspEI T'CCGG_A
Cuts at: 2550 3069 6687 2550
Size: 519 3618 2887
Fragments arranged by size:
3618 2887 519
BspHI T'CATG_A
Cuts at: 4913 6643 4913
Size: 1730 5294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 4379 844
Size: 1610 1925 3489
Fragments arranged by size:
3489 1925 1610
BsrBI CCG'CTC
Cuts at: 1394 1823 2761 4911 1394
Size: 429 938 2150 3507
Fragments arranged by size:
3507 2150 938 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2480 5519 5693 65
Size: 1183 1232 3039 174 1396
Fragments arranged by size:
3039 1396 1232 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3244 3563 3876 5668 5855 1700
Size: 110 1434 319 313 1792 187 2869
Fragments arranged by size:
2869 1792 1434 319 313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7024
BssSI C'ACGA_G
Cuts at: 5084 6096 5084
Size: 1012 6012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2751 794
Size: 1957 5067
BstBI TT'CG_AA
Cuts at: 5828 5828
Size: 7024
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7024
BstXI CCAn_nnnn'nTGG
Cuts at: 2526 2986 2526
Size: 460 6564
BstYI r'GATC_y
Cuts at: 1972 2226 2547 2725 3207 3759 4264 4354 4721
Size: 254 321 178 482 552 505 90 367
Cuts at: 4721 5106 5123 6575 6661 6673 7019 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 552 505 482 385 367 346
321 254 178 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2246 2246
Size: 7024
BtgI C'CryG_G
Cuts at: 513 1065 2004 2207 2772 2799 3750 513
Size: 552 939 203 565 27 951 3787
Fragments arranged by size:
3787 951 939 565 552 203 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6285
Fragments arranged by size:
6285 683 56
BtsI GCAGTG_nn'
Cuts at: 2749 2953 3234 3929 4540 5334 5354 2749
Size: 204 281 695 611 794 20 4419
Fragments arranged by size:
4419 794 695 611 281 204 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4078 4113 529
Size: 35 3514 35 3440
Fragments arranged by size:
3514 3440 35 35
DraI TTT'AAA
Cuts at: 4328 4328
Size: 7024
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2186 6031 809
Size: 507 870 3845 1802
Fragments arranged by size:
3845 1802 870 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2988 3255
Size: 54 1425 50 275 18 447 711 267
Cuts at: 3255 3423 4471 5360 6989 8
Size: 168 1048 889 1629 43
Fragments arranged by size:
1629 1425 1048 889 711 447 275 267
168 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4471 1537
Size: 275 2659 4090
Fragments arranged by size:
4090 2659 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 3018 4202 4954 1353
Size: 786 879 1184 752 3423
Fragments arranged by size:
3423 1184 879 786 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2287 5611 5985 6131 387
Size: 1064 138 698 3324 374 146 1280
Fragments arranged by size:
3324 1280 1064 698 374 146 138
EcoICRI GAG'CTC
Cuts at: 2893 3975 4200 2893
Size: 1082 225 5717
Fragments arranged by size:
5717 1082 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3080 3184 3430 3512 3851 3911 4016
Size: 393 1448 104 246 82 339 60 105
Cuts at: 4016 4127 4289 5087 5684 6471 6739 1239
Size: 111 162 798 597 787 268 1524
Fragments arranged by size:
1524 1448 798 787 597 393 339 268
246 162 111 105 104 82 60
EcoO109I rG'GnC_Cy
Cuts at: 2608 2971 3341 3713 2608
Size: 363 370 372 5919
Fragments arranged by size:
5919 372 370 363
EcoRI G'AATT_C
Cuts at: 2735 4333 4711 2735
Size: 1598 378 5048
Fragments arranged by size:
5048 1598 378
FspI TGC'GCA
Cuts at: 5530 5530
Size: 7024
HaeII r_GCGC'y
Cuts at: 1678 2115 2254 3251 6171 1678
Size: 437 139 997 2920 2531
Fragments arranged by size:
2920 2531 997 437 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3131 3163 5665 5697 5739
Size: 32 135 32 1559 32 2502 32 42
Cuts at: 5739 5771 1373
Size: 32 2626
Fragments arranged by size:
2626 2502 1559 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4368 4614 669
Size: 988 281 2430 246 3079
Fragments arranged by size:
3079 2430 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4277 748
Size: 3529 3495
HpaI GTT'AAC
Cuts at: 4614 4614
Size: 7024
KasI G'GCGC_C
Cuts at: 2111 2111
Size: 7024
KpnI G_GTAC'C
Cuts at: 4349 4349
Size: 7024
MfeI C'AATT_G
Cuts at: 2343 4623 2343
Size: 2280 4744
MluI A'CGCG_T
Cuts at: 6900 6900
Size: 7024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4104 6138 6322 1305
Size: 2799 2034 184 2007
Fragments arranged by size:
2799 2034 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3425 10
Size: 54 1425 343 1593 3609
Fragments arranged by size:
3609 1593 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2105 2681 2786 2798 3653 3842
Size: 648 843 96 576 105 12 855 189
Cuts at: 3842 4982 5341 5500 518
Size: 1140 359 159 2042
Fragments arranged by size:
2042 1140 855 843 648 576 359 189
159 105 96 12
MspA1I CmG'CkG
Cuts at: 1606 2068 2209 2359 2526 3868 3883 4075 4250
Size: 462 141 150 167 1342 15 192 175
Cuts at: 4250 4440 5117 6265 6510 1606
Size: 190 677 1148 245 2120
Fragments arranged by size:
2120 1342 1148 677 462 245 192 190
175 167 150 141 15
NaeI GCC'GGC
Cuts at: 3246 3565 3246
Size: 319 6705
NarI GG'CG_CC
Cuts at: 2112 2112
Size: 7024
NcoI C'CATG_G
Cuts at: 513 1065 2004 2772 3750 513
Size: 552 939 768 978 3787
Fragments arranged by size:
3787 978 939 768 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7024
NgoMIV G'CCGG_C
Cuts at: 3244 3563 3244
Size: 319 6705
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7024
NotI GC'GGCC_GC
Cuts at: 4471 4471
Size: 7024
NspI r_CATG'y
Cuts at: 1132 1229 2938 3724 3859 4382 5927 1132
Size: 97 1709 786 135 523 1545 2229
Fragments arranged by size:
2229 1709 1545 786 523 135 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7024
PciI A'CATG_T
Cuts at: 5923 5923
Size: 7024
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3751 1108
Size: 2643 4381
PmeI GTTT'AAAC
Cuts at: 4328 4328
Size: 7024
PmlI CAC'GTG
Cuts at: 3214 3214
Size: 7024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 6606 6638 5093
Size: 32 1481 32 5479
Fragments arranged by size:
5479 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2971 3713 2971
Size: 742 6282
PshAI GACnn'nnGTC
Cuts at: 1613 4169 1613
Size: 2556 4468
PsiI TTA'TAA
Cuts at: 4594 4594
Size: 7024
PspOMI G'GGCC_C
Cuts at: 3340 3914 3340
Size: 574 6450
PstI C_TGCA'G
Cuts at: 830 2172 2430 2589 2756 3320 3816 4220 4376
Size: 1342 258 159 167 564 496 404 156
Cuts at: 4376 830
Size: 3478
Fragments arranged by size:
3478 1342 564 496 404 258 167 159
156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7024
PvuII CAG'CTG
Cuts at: 2359 3868 4075 4250 2359
Size: 1509 207 175 5133
Fragments arranged by size:
5133 1509 207 175
SacI G_AGCT'C
Cuts at: 2895 3977 4202 2895
Size: 1082 225 5717
Fragments arranged by size:
5717 1082 225
SacII CC_GC'GG
Cuts at: 2210 2210
Size: 7024
SalI G'TCGA_C
Cuts at: 1936 4366 1936
Size: 2430 4594
SanDI GG'GwC_CC
Cuts at: 3713 3713
Size: 7024
SapI GCTCTTCn'nnn_
Cuts at: 2139 2139
Size: 7024
SbfI CC_TGCA'GG
Cuts at: 2589 4376 2589
Size: 1787 5237
ScaI AGT'ACT
Cuts at: 2387 5272 6984 2387
Size: 2885 1712 2427
Fragments arranged by size:
2885 2427 1712
SexAI A'CCwGG_T
Cuts at: 2982 3141 2982
Size: 159 6865
SfcI C'TryA_G
Cuts at: 826 1045 2168 2335 2426 2585 2752 3316 3589
Size: 219 1123 167 91 159 167 564 273
Cuts at: 3589 3812 4216 4372 5507 6188 6379 826
Size: 223 404 156 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 681 564 404 273 223
219 191 167 167 159 156 91
SfoI GGC'GCC
Cuts at: 2113 2113
Size: 7024
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7024
SmaI CCC'GGG
Cuts at: 1872 2969 4351 1872
Size: 1097 1382 4545
Fragments arranged by size:
4545 1382 1097
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2816 3179 3383 4067 5128 6029
Size: 197 926 873 363 204 684 1061 901
Cuts at: 6029 6291 820
Size: 262 1553
Fragments arranged by size:
1553 1061 926 901 873 684 363 262
204 197
SnaBI TAC'GTA
Cuts at: 493 4815 493
Size: 4322 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7024
SphI G_CATG'C
Cuts at: 1132 2938 3724 4382 1132
Size: 1806 786 658 3774
Fragments arranged by size:
3774 1806 786 658
SspI AAT'ATT
Cuts at: 5 52 4948 5
Size: 47 4896 2081
Fragments arranged by size:
4896 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7024
StyI C'CwwG_G
Cuts at: 513 1065 2004 2772 3483 3750 4463 513
Size: 552 939 768 711 267 713 3074
Fragments arranged by size:
3074 939 768 713 711 552 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2675 2730 3821 5051 5068 5221 5406
Size: 1255 1095 55 1091 1230 17 153 185
Cuts at: 5406 5851 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1230 1095 1091 445 185 153
55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 2871 5270
Size: 274 80 33 51 1443 408 486 2399
Cuts at: 5270 6982 96
Size: 1712 138
Fragments arranged by size:
2399 1712 1443 486 408 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 6984 7016 3749
Size: 32 3203 32 3757
Fragments arranged by size:
3757 3203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7024
XbaI T'CTAG_A
Cuts at: 4360 4360
Size: 7024
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 3139 2068
Size: 1071 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7024
XmaI C'CCGG_G
Cuts at: 1870 2967 4349 1870
Size: 1097 1382 4545
Fragments arranged by size:
4545 1382 1097
XmnI GAAnn'nnTTC
Cuts at: 2352 2921 5153 2352
Size: 569 2232 4223
Fragments arranged by size:
4223 2232 569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4303 4832 276
Size: 53 83 186 936 2769 529 2468
Fragments arranged by size:
2769 2468 936 529 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SfiI SrfI
SwaI