Restriction map for
pFN21AA1682
(Circular) (Six-base) MAPSORT of: pFN21AA1682.seq Check: 488 from: 1 to: 6946
pFN21AA1682 6946 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4756 278
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4289 1937
Size: 2352 4594
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 2420 2775 5009 6393 6661 1632
Size: 569 219 355 2234 1384 268 1917
Fragments arranged by size:
2234 1917 1384 569 355 268 219
AfeI AGC'GCT
Cuts at: 2771 3929 2771
Size: 1158 5788
AflII C'TTAA_G
Cuts at: 820 1017 2627 820
Size: 197 1610 5139
Fragments arranged by size:
5139 1610 197
AflIII A'CryG_T
Cuts at: 1203 5845 6822 1203
Size: 4642 977 1327
Fragments arranged by size:
4642 1327 977
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 5675 5675
Size: 6946
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6946
AlwNI CAG_nnn'CTG
Cuts at: 2591 4176 4689 6261 2591
Size: 1585 513 1572 3276
Fragments arranged by size:
3276 1585 1572 513
ApaI G_GGCC'C
Cuts at: 3906 3925 3906
Size: 19 6927
ApaLI G'TGCA_C
Cuts at: 3392 5003 6159 3392
Size: 1611 1156 4179
Fragments arranged by size:
4179 1611 1156
ApoI r'AATT_y
Cuts at: 1493 2088 2901 3002 3038 3416 4255 4481 4633
Size: 595 813 101 36 378 839 226 152
Cuts at: 4633 6742 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 839 813 595 378 226 152
101 36
AseI AT'TA_AT
Cuts at: 160 1032 3801 5500 160
Size: 872 2769 1699 1606
Fragments arranged by size:
2769 1699 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 4271 1870
Size: 73 2328 4545
Fragments arranged by size:
4545 2328 73
AvrII C'CTAG_G
Cuts at: 3961 4234 3961
Size: 273 6673
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 4267 5722 618
Size: 325 217 627 252 2228 1455 1842
Fragments arranged by size:
2228 1842 1455 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 3819 3906 3925 4265 1391
Size: 2428 87 19 340 4072
Fragments arranged by size:
4072 2428 340 87 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2955 4203 928
Size: 971 1056 1248 3671
Fragments arranged by size:
3671 1248 1056 971
BbvCI CC'TCA_GC
Cuts at: 4074 4091 4074
Size: 17 6929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5137 5171 1903
Size: 34 50 34 3116 34 3678
Fragments arranged by size:
3678 3116 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3526 4838 6048 3526
Size: 1312 1210 4424
Fragments arranged by size:
4424 1312 1210
BclI T'GATC_A
Cuts at: 1548 2347 1548
Size: 799 6147
BfrBI ATG'CAT
Cuts at: 3185 3185
Size: 6946
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4070 5557 136
Size: 107 122 71 631 605 2398 1487 1525
Fragments arranged by size:
2398 1525 1487 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6941 6941
Size: 6946
BlpI GC'TnA_GC
Cuts at: 4363 4363
Size: 6946
Bme1580I G_kGCm'C
Cuts at: 1790 2827 3396 3906 3925 5007 6163 1790
Size: 1037 569 510 19 1082 1156 2573
Fragments arranged by size:
2573 1156 1082 1037 569 510 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4677 5635 442
Size: 416 53 83 399 24 3260 958 1753
Fragments arranged by size:
3260 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4121 5606 1239
Size: 2882 1485 2579
Fragments arranged by size:
2882 2579 1485
Bpu10I CC'TnA_GC
Cuts at: 4074 4091 4074
Size: 17 6929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5071 5936 6234 5071
Size: 865 298 5783
Fragments arranged by size:
5783 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5609 882
Size: 617 4110 2219
Fragments arranged by size:
4110 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2074 4737 493
Size: 711 870 2663 2702
Fragments arranged by size:
2702 2663 870 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4753 5135 5770 275
Size: 53 83 186 936 3220 382 635 1451
Fragments arranged by size:
3220 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5379 5777 6051 6198 6609 5379
Size: 398 274 147 411 5716
Fragments arranged by size:
5716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2509 2539 1178
Size: 30 165 30 1106 30 5585
Fragments arranged by size:
5585 1106 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3191 3359 1186
Size: 2005 168 4773
Fragments arranged by size:
4773 2005 168
BseYI C'CCAG_C
Cuts at: 2008 3924 6149 6645 2008
Size: 1916 2225 496 2309
Fragments arranged by size:
2309 2225 1916 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 2556 2786 3602 1163
Size: 573 482 338 230 816 4507
Fragments arranged by size:
4507 816 573 482 338 230
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3396 3819 5007 5092 6163 3396
Size: 423 1188 85 1071 4179
Fragments arranged by size:
4179 1188 1071 423 85
BsiWI C'GTAC_G
Cuts at: 2070 2074 2070
Size: 4 6942
BsmI GAATG_Cn'
Cuts at: 3187 3672 4457 4550 3187
Size: 485 785 93 5583
Fragments arranged by size:
5583 785 485 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2827 3396 3819 3906 3925 4265 5007
Size: 399 1037 569 423 87 19 340 742
Cuts at: 5007 5092 6163 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1037 742 569 423 399 340
87 85 19
BspEI T'CCGG_A
Cuts at: 6609 6609
Size: 6946
BspHI T'CATG_A
Cuts at: 4835 6565 4835
Size: 1730 5216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4301 844
Size: 3457 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 1394
Size: 429 3010 3507
Fragments arranged by size:
3507 3010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3605 5441 5615 65
Size: 1183 2357 1836 174 1396
Fragments arranged by size:
2357 1836 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5590 5777 1700
Size: 110 3780 187 2869
Fragments arranged by size:
3780 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6946
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3391 794
Size: 2597 4349
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 4276 4643 5028 5045 6497 6583 6595 6941
Size: 2304 367 385 17 1452 86 12 346
Cuts at: 6941 1972
Size: 1977
Fragments arranged by size:
2304 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2082 2082
Size: 6946
BtgI C'CryG_G
Cuts at: 513 1065 3874 513
Size: 552 2809 3585
Fragments arranged by size:
3585 2809 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6207
Fragments arranged by size:
6207 683 56
BtsI GCAGTG_nn'
Cuts at: 2428 2688 2843 3238 4462 5256 5276 2428
Size: 260 155 395 1224 794 20 4098
Fragments arranged by size:
4098 1224 794 395 260 155 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3263 3298 529
Size: 35 2699 35 4177
Fragments arranged by size:
4177 2699 35 35
DraI TTT'AAA
Cuts at: 3007 4250 3007
Size: 1243 5703
DraIII CAC_nnn'GTG
Cuts at: 3567 3567
Size: 6946
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3442 5953 809
Size: 507 2126 2511 1802
Fragments arranged by size:
2511 2126 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3295 3970 3978
Size: 54 1425 50 275 18 1465 675 8
Cuts at: 3978 4206 4393 5282 6911 8
Size: 228 187 889 1629 43
Fragments arranged by size:
1629 1465 1425 889 675 275 228 187
54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2472 2751 2754 4876 1353
Size: 783 336 279 3 2122 3423
Fragments arranged by size:
3423 2122 783 336 279 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3317 5533 5907 6053 387
Size: 1064 138 1728 2216 374 146 1280
Fragments arranged by size:
2216 1728 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3817 3817
Size: 6946
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2420 2775 4121 5009 5606 6393
Size: 393 569 219 355 1346 888 597 787
Cuts at: 6393 6661 1239
Size: 268 1524
Fragments arranged by size:
1524 1346 888 787 597 569 393 355
268 219
EcoO109I rG'GnC_Cy
Cuts at: 2047 3902 4063 4078 2047
Size: 1855 161 15 4915
Fragments arranged by size:
4915 1855 161 15
EcoRI G'AATT_C
Cuts at: 3416 4255 4633 3416
Size: 839 378 5729
Fragments arranged by size:
5729 839 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2147 2269 2908 3564 4177 2077
Size: 70 122 639 656 613 4846
Fragments arranged by size:
4846 656 639 613 122 70
FspI TGC'GCA
Cuts at: 5452 5452
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 2773 3931 6093 1678
Size: 1095 1158 2162 2531
Fragments arranged by size:
2531 2162 1158 1095
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 3170 3202 5587
Size: 32 135 32 683 32 883 32 2385
Cuts at: 5587 5619 5661 5693 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2385 883 683 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2692 4290 4536 669
Size: 988 281 754 1598 246 3079
Fragments arranged by size:
3079 1598 988 754 281 246
HindIII A'AGCT_T
Cuts at: 748 4182 748
Size: 3434 3512
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3054 3213 3920 6060 6244 1305
Size: 1749 159 707 2140 184 2007
Fragments arranged by size:
2140 2007 1749 707 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3297 3972 3980 4208 10
Size: 54 1425 343 1465 675 8 228 2748
Fragments arranged by size:
2748 1465 1425 675 343 228 54 8
MslI CAynn'nnrTG
Cuts at: 518 1166 4904 5263 5422 518
Size: 648 3738 359 159 2042
Fragments arranged by size:
3738 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2155 3087 3388 3849 4362 5039 6187 6432
Size: 549 932 301 461 513 677 1148 245
Cuts at: 6432 1606
Size: 2120
Fragments arranged by size:
2120 1148 932 677 549 513 461 301
245
NcoI C'CATG_G
Cuts at: 513 1065 3874 513
Size: 552 2809 3585
Fragments arranged by size:
3585 2809 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6946
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6946
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NsiI A_TGCA'T
Cuts at: 3187 3187
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 4304 5849 1132
Size: 97 3075 1545 2229
Fragments arranged by size:
3075 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 3765 3765
Size: 6946
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6946
PciI A'CATG_T
Cuts at: 5845 5845
Size: 6946
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4176 1108
Size: 3068 3878
PfoI T'CCnGG_A
Cuts at: 2190 2190
Size: 6946
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 4516 4516
Size: 6946
PspOMI G'GGCC_C
Cuts at: 3902 3921 3902
Size: 19 6927
PstI C_TGCA'G
Cuts at: 830 2979 3245 4298 830
Size: 2149 266 1053 3478
Fragments arranged by size:
3478 2149 1053 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 2155 3087 3388 3849 2155
Size: 932 301 461 5252
Fragments arranged by size:
5252 932 461 301
SacI G_AGCT'C
Cuts at: 3819 3819
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SbfI CC_TGCA'GG
Cuts at: 2979 4298 2979
Size: 1319 5627
ScaI AGT'ACT
Cuts at: 2178 3034 5194 6906 2178
Size: 856 2160 1712 2218
Fragments arranged by size:
2218 2160 1712 856
SfcI C'TryA_G
Cuts at: 826 1045 2975 3241 4294 5429 6110 6301 826
Size: 219 1930 266 1053 1135 681 191 1471
Fragments arranged by size:
1930 1471 1135 1053 681 266 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 5050 5951 6213 820
Size: 197 926 684 2423 901 262 1553
Fragments arranged by size:
2423 1553 926 901 684 262 197
SnaBI TAC'GTA
Cuts at: 493 2074 4737 493
Size: 1581 2663 2702
Fragments arranged by size:
2702 2663 1581
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 4304 1132
Size: 3172 3774
SspI AAT'ATT
Cuts at: 5 52 4870 5
Size: 47 4818 2081
Fragments arranged by size:
4818 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6946
StyI C'CwwG_G
Cuts at: 513 1065 2043 3874 3961 4234 4385 513
Size: 552 978 1831 87 273 151 3074
Fragments arranged by size:
3074 1831 978 552 273 151 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 3281 4973 4990 5143 5328 5773
Size: 1255 669 1032 1692 17 153 185 445
Cuts at: 5773 325
Size: 1498
Fragments arranged by size:
1692 1498 1255 1032 669 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2176 2673 3032
Size: 274 80 33 51 1443 199 497 359
Cuts at: 3032 5192 6904 96
Size: 2160 1712 138
Fragments arranged by size:
2160 1712 1443 497 359 274 199 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2485 2517 2734 2766 6906 6938 2485
Size: 32 217 32 4140 32 2493
Fragments arranged by size:
4140 2493 217 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6946
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2799 3962 2799
Size: 1163 5783
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 5075 5075
Size: 6946
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4754 276
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BplI BsmBI BstXI
BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI