Restriction map for
pFN21AA1680
(Circular) (Six-base) MAPSORT of: pFN21AA1680.seq Check: 7931 from: 1 to: 7405
pFN21AA1680 7405 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5215 278
Size: 53 83 186 936 3679 2468
Fragments arranged by size:
3679 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4748 1937
Size: 2811 4594
Acc65I G'GTAC_C
Cuts at: 4726 4726
Size: 7405
AclI AA'CG_TT
Cuts at: 1563 5532 5905 1563
Size: 3969 373 3063
Fragments arranged by size:
3969 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2760 2817 4014 4070 4406 4431 4514 5468
Size: 1128 57 1197 56 336 25 83 954
Cuts at: 5468 6852 7120 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1197 1128 954 336 268 83
57 56 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7208
AflIII A'CryG_T
Cuts at: 1203 3220 4626 6304 7281 1203
Size: 2017 1406 1678 977 1327
Fragments arranged by size:
2017 1678 1406 1327 977
AgeI A'CCGG_T
Cuts at: 6236 6236
Size: 7405
AhdI GACnn_n'nnGTC
Cuts at: 6134 6134
Size: 7405
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7405
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4694 1184
Size: 3510 3895
AlwNI CAG_nnn'CTG
Cuts at: 2311 5148 6720 2311
Size: 2837 1572 2996
Fragments arranged by size:
2996 2837 1572
ApaLI G'TGCA_C
Cuts at: 5462 6618 5462
Size: 1156 6249
ApoI r'AATT_y
Cuts at: 1493 2573 2627 2780 3575 3707 4714 4940 5092
Size: 1080 54 153 795 132 1007 226 152
Cuts at: 5092 7201 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1080 1007 795 226 153 152
132 54
AseI AT'TA_AT
Cuts at: 160 1032 5959 160
Size: 872 4927 1606
Fragments arranged by size:
4927 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7405
AvaI C'yCGr_G
Cuts at: 1870 1943 4730 1870
Size: 73 2787 4545
Fragments arranged by size:
4545 2787 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2606 2639 2606
Size: 33 7372
BamHI G'GATC_C
Cuts at: 3231 4735 5102 3231
Size: 1504 367 5534
Fragments arranged by size:
5534 1504 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4726 6181 618
Size: 325 217 627 2939 1455 1842
Fragments arranged by size:
2939 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4490 4521 4724 1391
Size: 3099 31 203 4072
Fragments arranged by size:
4072 3099 203 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2083 2350 2508 3587 928
Size: 971 184 267 158 1079 4746
Fragments arranged by size:
4746 1079 971 267 184 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5596 5630 1903
Size: 34 3659 34 3678
Fragments arranged by size:
3678 3659 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5297 6507 5297
Size: 1210 6195
BclI T'GATC_A
Cuts at: 1548 3274 3760 1548
Size: 1726 486 5193
Fragments arranged by size:
5193 1726 486
BfrBI ATG'CAT
Cuts at: 2822 2822
Size: 7405
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6016 136
Size: 107 122 71 631 605 4344 1525
Fragments arranged by size:
4344 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2390 7400 2390
Size: 5010 2395
BlpI GC'TnA_GC
Cuts at: 4822 4822
Size: 7405
Bme1580I G_kGCm'C
Cuts at: 1790 5466 6622 1790
Size: 3676 1156 2573
Fragments arranged by size:
3676 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3969 1318
Size: 2651 4754
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5136 6094 442
Size: 416 53 83 399 24 3719 958 1753
Fragments arranged by size:
3719 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6065 1239
Size: 4826 2579
Bpu10I CC'TnA_GC
Cuts at: 2161 2238 3714 2161
Size: 77 1476 5852
Fragments arranged by size:
5852 1476 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2140 4028 5530 6395 6693 2140
Size: 1888 1502 865 298 2852
Fragments arranged by size:
2852 1888 1502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2044 6068 882
Size: 617 545 4024 2219
Fragments arranged by size:
4024 2219 617 545
BsaAI yAC'GTr
Cuts at: 493 1204 5196 493
Size: 711 3992 2702
Fragments arranged by size:
3992 2702 711
BsaBI GATnn'nnATC
Cuts at: 7399 7399
Size: 7405
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5212 5594 6229 275
Size: 53 83 186 936 3679 382 635 1451
Fragments arranged by size:
3679 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3016 5838 6236 6510 6657 7068 3016
Size: 2822 398 274 147 411 3353
Fragments arranged by size:
3353 2822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7180
Fragments arranged by size:
7180 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2197 3391 1186
Size: 1011 1194 5200
Fragments arranged by size:
5200 1194 1011
BseYI C'CCAG_C
Cuts at: 3160 4096 6608 7104 3160
Size: 936 2512 496 3461
Fragments arranged by size:
3461 2512 936 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3871 3960 1163
Size: 573 2135 89 4608
Fragments arranged by size:
4608 2135 573 89
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4855 5616 6644 1540
Size: 275 186 2854 761 1028 2301
Fragments arranged by size:
2854 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2284 2427 4490 5466 5551 6622 2284
Size: 143 2063 976 85 1071 3067
Fragments arranged by size:
3067 2063 1071 976 143 85
BsmI GAATG_Cn'
Cuts at: 3798 4037 4570 4916 5009 3798
Size: 239 533 346 93 6194
Fragments arranged by size:
6194 533 346 239 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3643 3643
Size: 7405
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2427 4490 4521 4724 5466 5551
Size: 399 494 143 2063 31 203 742 85
Cuts at: 5551 6622 1391
Size: 1071 2174
Fragments arranged by size:
2174 2063 1071 742 494 399 203 143
85 31
BspEI T'CCGG_A
Cuts at: 7068 7068
Size: 7405
BspHI T'CATG_A
Cuts at: 5294 7024 5294
Size: 1730 5675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3079 3473 4578 4760 844
Size: 2235 394 1105 182 3489
Fragments arranged by size:
3489 2235 1105 394 182
BsrBI CCG'CTC
Cuts at: 1394 1823 5292 1394
Size: 429 3469 3507
Fragments arranged by size:
3507 3469 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5900 6074 65
Size: 1183 4652 174 1396
Fragments arranged by size:
4652 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6049 6236 1700
Size: 110 4239 187 2869
Fragments arranged by size:
4239 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4629 96
Size: 4533 2872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7405
BssSI C'ACGA_G
Cuts at: 5465 6477 5465
Size: 1012 6393
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3320 794
Size: 2526 4879
BstBI TT'CG_AA
Cuts at: 6209 6209
Size: 7405
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7405
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 4700 2611
Size: 2089 5316
BstYI r'GATC_y
Cuts at: 1972 2390 3231 3533 4735 5102 5487 5504 6956
Size: 418 841 302 1202 367 385 17 1452
Cuts at: 6956 7042 7054 7400 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1202 841 418 385 367 346
302 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5914
Fragments arranged by size:
5914 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6666
Fragments arranged by size:
6666 683 56
BtsI GCAGTG_nn'
Cuts at: 3402 4921 5715 5735 3402
Size: 1519 794 20 5072
Fragments arranged by size:
5072 1519 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7405
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7370
DraI TTT'AAA
Cuts at: 4709 4709
Size: 7405
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6412 809
Size: 507 5096 1802
Fragments arranged by size:
5096 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2866 4852 5741
Size: 54 1425 50 275 18 1036 1986 889
Cuts at: 5741 7370 8
Size: 1629 43
Fragments arranged by size:
1986 1629 1425 1036 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4852 1537
Size: 275 3040 4090
Fragments arranged by size:
4090 3040 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 3914 3990 4086 5335 1353
Size: 741 1820 76 96 1249 3423
Fragments arranged by size:
3423 1820 1249 741 96 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5992 6366 6512 387
Size: 1064 138 4403 374 146 1280
Fragments arranged by size:
4403 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4488 4488
Size: 7405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2760 2817 4014 4070 4406 4431 4514
Size: 393 1128 57 1197 56 336 25 83
Cuts at: 4514 5468 6065 6852 7120 1239
Size: 954 597 787 268 1524
Fragments arranged by size:
1524 1197 1128 954 787 597 393 336
268 83 57 56 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3825 3825
Size: 7405
EcoRI G'AATT_C
Cuts at: 2627 4714 5092 2627
Size: 2087 378 4940
Fragments arranged by size:
4940 2087 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 4446 2461
Size: 1985 5420
FspI TGC'GCA
Cuts at: 5911 5911
Size: 7405
HaeII r_GCGC'y
Cuts at: 1678 6552 1678
Size: 4874 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2792 2824 2867
Size: 32 135 32 431 32 757 32 43
Cuts at: 2867 2899 4019 4051 4237 4269 4599 4631 6046
Size: 32 1120 32 186 32 330 32 1415
Cuts at: 6046 6078 6120 6152 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1415 1120 757 431 330 186 135
43 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2532 4355 4749 4995 669
Size: 988 281 594 1823 394 246 3079
Fragments arranged by size:
3079 1823 988 594 394 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7405
HpaI GTT'AAC
Cuts at: 4995 4995
Size: 7405
KpnI G_GTAC'C
Cuts at: 4730 4730
Size: 7405
MfeI C'AATT_G
Cuts at: 5004 5004
Size: 7405
MluI A'CGCG_T
Cuts at: 7281 7281
Size: 7405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6519 6703 1305
Size: 5214 184 2007
Fragments arranged by size:
5214 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2868 10
Size: 54 1425 343 1036 4547
Fragments arranged by size:
4547 1425 1036 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3306 5363 5722 5881 518
Size: 648 2140 2057 359 159 2042
Fragments arranged by size:
2140 2057 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4054 4319 4821 5498 6646 6891 1606
Size: 2448 265 502 677 1148 245 2120
Fragments arranged by size:
2448 2120 1148 677 502 265 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5914
Fragments arranged by size:
5914 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7405
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7405
NotI GC'GGCC_GC
Cuts at: 4852 4852
Size: 7405
NsiI A_TGCA'T
Cuts at: 2824 2824
Size: 7405
NspI r_CATG'y
Cuts at: 1132 1229 3224 4630 4763 6308 1132
Size: 97 1995 1406 133 1545 2229
Fragments arranged by size:
2229 1995 1545 1406 133 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7405
PciI A'CATG_T
Cuts at: 3220 4626 6304 3220
Size: 1406 1678 4321
Fragments arranged by size:
4321 1678 1406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7405
PmeI GTTT'AAAC
Cuts at: 4709 4709
Size: 7405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5474 5506 6987 7019 5474
Size: 32 1481 32 5860
Fragments arranged by size:
5860 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3948 1613
Size: 2335 5070
PsiI TTA'TAA
Cuts at: 2450 4975 2450
Size: 2525 4880
PstI C_TGCA'G
Cuts at: 830 3409 4053 4757 830
Size: 2579 644 704 3478
Fragments arranged by size:
3478 2579 704 644
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7405
PvuII CAG'CTG
Cuts at: 4054 4054
Size: 7405
SacI G_AGCT'C
Cuts at: 4490 4490
Size: 7405
SalI G'TCGA_C
Cuts at: 1936 4747 1936
Size: 2811 4594
SapI GCTCTTCn'nnn_
Cuts at: 3990 4086 3990
Size: 96 7309
SbfI CC_TGCA'GG
Cuts at: 4757 4757
Size: 7405
ScaI AGT'ACT
Cuts at: 5653 7365 5653
Size: 1712 5693
SexAI A'CCwGG_T
Cuts at: 2287 2287
Size: 7405
SfcI C'TryA_G
Cuts at: 826 1045 2591 3405 4049 4753 5888 6569 6760
Size: 219 1546 814 644 704 1135 681 191
Cuts at: 6760 826
Size: 1471
Fragments arranged by size:
1546 1471 1135 814 704 681 644 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7405
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7405
SmaI CCC'GGG
Cuts at: 1872 4732 1872
Size: 2860 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2155 4043 5509 6410 6672 820
Size: 197 926 212 1888 1466 901 262 1553
Fragments arranged by size:
1888 1553 1466 926 901 262 212 197
SnaBI TAC'GTA
Cuts at: 493 5196 493
Size: 4703 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7405
SphI G_CATG'C
Cuts at: 1132 4763 1132
Size: 3631 3774
SspI AAT'ATT
Cuts at: 5 52 2055 5329 5
Size: 47 2003 3274 2081
Fragments arranged by size:
3274 2081 2003 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7405
StyI C'CwwG_G
Cuts at: 513 1065 2004 3176 4844 513
Size: 552 939 1172 1668 3074
Fragments arranged by size:
3074 1668 1172 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4618 5432 5449 5602 5787 6232 325
Size: 1255 3038 814 17 153 185 445 1498
Fragments arranged by size:
3038 1498 1255 814 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4204 4629 5651
Size: 274 80 33 51 1443 2227 425 1022
Cuts at: 5651 7363 96
Size: 1712 138
Fragments arranged by size:
2227 1712 1443 1022 425 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3958 3990 7365 7397 3958
Size: 32 3375 32 3966
Fragments arranged by size:
3966 3375 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7405
XbaI T'CTAG_A
Cuts at: 4741 4741
Size: 7405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3813 3813
Size: 7405
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7405
XmaI C'CCGG_G
Cuts at: 1870 4730 1870
Size: 2860 4545
XmnI GAAnn'nnTTC
Cuts at: 2347 5534 2347
Size: 3187 4218
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5213 276
Size: 53 83 186 936 3679 2468
Fragments arranged by size:
3679 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI