Restriction map for
pFN21AA1673
(Circular) (Six-base) MAPSORT of: pFN21AA1673.seq Check: 6879 from: 1 to: 6841
pFN21AA1673 6841 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4651 278
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4184 1937
Size: 2247 4594
Acc65I G'GTAC_C
Cuts at: 4162 4162
Size: 6841
AclI AA'CG_TT
Cuts at: 1563 4140 4968 5341 1563
Size: 2577 828 373 3063
Fragments arranged by size:
3063 2577 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3313 4904 6288 6556 1632
Size: 1681 1591 1384 268 1917
Fragments arranged by size:
1917 1681 1591 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6644
AflIII A'CryG_T
Cuts at: 1203 5740 6717 1203
Size: 4537 977 1327
Fragments arranged by size:
4537 1327 977
AgeI A'CCGG_T
Cuts at: 5672 5672
Size: 6841
AhdI GACnn_n'nnGTC
Cuts at: 5570 5570
Size: 6841
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6841
AlwNI CAG_nnn'CTG
Cuts at: 2514 4584 6156 2514
Size: 2070 1572 3199
Fragments arranged by size:
3199 2070 1572
ApaI G_GGCC'C
Cuts at: 3973 3973
Size: 6841
ApaLI G'TGCA_C
Cuts at: 4898 6054 4898
Size: 1156 5685
ApoI r'AATT_y
Cuts at: 1493 2629 3013 3986 4076 4150 4376 4528 6637
Size: 1136 384 973 90 74 226 152 2109
Cuts at: 6637 1493
Size: 1697
Fragments arranged by size:
2109 1697 1136 973 384 226 152 90
74
AseI AT'TA_AT
Cuts at: 160 1032 5395 160
Size: 872 4363 1606
Fragments arranged by size:
4363 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
AvaI C'yCGr_G
Cuts at: 1870 1943 2905 4166 1870
Size: 73 962 1261 4545
Fragments arranged by size:
4545 1261 962 73
BamHI G'GATC_C
Cuts at: 4171 4538 4171
Size: 367 6474
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4162 5617 618
Size: 325 217 627 2375 1455 1842
Fragments arranged by size:
2375 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2906 3973 4160 1391
Size: 1515 1067 187 4072
Fragments arranged by size:
4072 1515 1067 187
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3234 928
Size: 971 1335 4535
Fragments arranged by size:
4535 1335 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5032 5066 1903
Size: 34 3095 34 3678
Fragments arranged by size:
3678 3095 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4733 5943 4733
Size: 1210 5631
BclI T'GATC_A
Cuts at: 1548 3943 1548
Size: 2395 4446
BfrBI ATG'CAT
Cuts at: 3506 3554 3558 3506
Size: 48 4 6789
Fragments arranged by size:
6789 48 4
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5452 136
Size: 107 122 71 631 605 3780 1525
Fragments arranged by size:
3780 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2223 6836 2223
Size: 4613 2228
BlpI GC'TnA_GC
Cuts at: 4258 4258
Size: 6841
Bme1580I G_kGCm'C
Cuts at: 1790 3877 3973 4902 6058 1790
Size: 2087 96 929 1156 2573
Fragments arranged by size:
2573 2087 1156 929 96
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6841
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 2460 4572
Size: 416 53 83 399 24 638 405 2112
Cuts at: 4572 5530 442
Size: 958 1753
Fragments arranged by size:
2112 1753 958 638 416 405 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2400 2920 3023 3182 3378 5501 1239
Size: 942 219 520 103 159 196 2123 2579
Fragments arranged by size:
2579 2123 942 520 219 196 159 103
Bpu10I CC'TnA_GC
Cuts at: 3801 3801
Size: 6841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4966 5831 6129 4966
Size: 865 298 5678
Fragments arranged by size:
5678 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2763 5504 882
Size: 617 1264 2741 2219
Fragments arranged by size:
2741 2219 1264 617
BsaAI yAC'GTr
Cuts at: 493 1204 4632 493
Size: 711 3428 2702
Fragments arranged by size:
3428 2702 711
BsaBI GATnn'nnATC
Cuts at: 6835 6835
Size: 6841
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4648 5030 5665 275
Size: 53 83 186 936 3115 382 635 1451
Fragments arranged by size:
3115 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3127 5274 5672 5946 6093 6504 3127
Size: 2147 398 274 147 411 3464
Fragments arranged by size:
3464 2147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2016 2046 1178
Size: 30 165 30 613 30 5973
Fragments arranged by size:
5973 613 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2530 2664 2881 1186
Size: 1344 134 217 5146
Fragments arranged by size:
5146 1344 217 134
BseYI C'CCAG_C
Cuts at: 2120 2171 2928 3056 3782 3824 4044 6044 6540
Size: 51 757 128 726 42 220 2000 496
Cuts at: 6540 2120
Size: 2421
Fragments arranged by size:
2421 2000 757 726 496 220 128 51
42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3557 1163
Size: 573 1821 4447
Fragments arranged by size:
4447 1821 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4291 5052 6080 1540
Size: 275 186 2290 761 1028 2301
Fragments arranged by size:
2301 2290 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2617 4902 4987 6058 2617
Size: 2285 85 1071 3400
Fragments arranged by size:
3400 2285 1071 85
BsmI GAATG_Cn'
Cuts at: 3506 4352 4445 3506
Size: 846 93 5902
Fragments arranged by size:
5902 846 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2499 2499
Size: 6841
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2617 2906 3877 3973 4160 4902 4987
Size: 399 827 289 971 96 187 742 85
Cuts at: 4987 6058 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 971 827 742 399 289 187
96 85
BspEI T'CCGG_A
Cuts at: 3127 6504 3127
Size: 3377 3464
BspHI T'CATG_A
Cuts at: 4730 6460 4730
Size: 1730 5111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4196 844
Size: 3352 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4728 1394
Size: 429 2905 3507
Fragments arranged by size:
3507 2905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2392 5336 5510 65
Size: 1183 1144 2944 174 1396
Fragments arranged by size:
2944 1396 1183 1144 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5485 5672 1700
Size: 110 3675 187 2869
Fragments arranged by size:
3675 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6841
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6841
BssSI C'ACGA_G
Cuts at: 4062 4901 5913 4062
Size: 839 1012 4990
Fragments arranged by size:
4990 1012 839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6841
BstBI TT'CG_AA
Cuts at: 5645 5645
Size: 6841
BstEII G'GTnAC_C
Cuts at: 1184 4106 1184
Size: 2922 3919
BstYI r'GATC_y
Cuts at: 1972 2223 4171 4538 4923 4940 6392 6478 6490
Size: 251 1948 367 385 17 1452 86 12
Cuts at: 6490 6836 1972
Size: 346 1977
Fragments arranged by size:
1977 1948 1452 385 367 346 251 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2855 3283 513
Size: 552 939 851 428 4071
Fragments arranged by size:
4071 939 851 552 428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3758 487
Size: 683 56 2532 3570
Fragments arranged by size:
3570 2532 683 56
BtsI GCAGTG_nn'
Cuts at: 2173 3322 4357 5151 5171 2173
Size: 1149 1035 794 20 3843
Fragments arranged by size:
3843 1149 1035 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6806
DraI TTT'AAA
Cuts at: 4145 4145
Size: 6841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5848 809
Size: 507 4532 1802
Fragments arranged by size:
4532 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4288 5177
Size: 54 1425 50 275 18 1450 1008 889
Cuts at: 5177 6806 8
Size: 1629 43
Fragments arranged by size:
1629 1450 1425 1008 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4288 1537
Size: 275 2476 4090
Fragments arranged by size:
4090 2476 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2899 3303 4771 1353
Size: 1546 404 1468 3423
Fragments arranged by size:
3423 1546 1468 404
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3399 5428 5802 5948 387
Size: 1064 138 1810 2029 374 146 1280
Fragments arranged by size:
2029 1810 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2400 2920 3023 3182 3313 3378
Size: 393 549 219 520 103 159 131 65
Cuts at: 3378 4904 5501 6288 6556 1239
Size: 1526 597 787 268 1524
Fragments arranged by size:
1526 1524 787 597 549 520 393 268
219 159 131 103 65
EcoNI CCTnn'n_nnAGG
Cuts at: 3495 4094 3495
Size: 599 6242
EcoO109I rG'GnC_Cy
Cuts at: 2587 3969 2587
Size: 1382 5459
EcoRI G'AATT_C
Cuts at: 4076 4150 4528 4076
Size: 74 378 6389
Fragments arranged by size:
6389 378 74
FspI TGC'GCA
Cuts at: 5347 5347
Size: 6841
HaeII r_GCGC'y
Cuts at: 1678 2534 5988 1678
Size: 856 3454 2531
Fragments arranged by size:
3454 2531 856
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 2671 2703 3140
Size: 32 135 32 826 32 241 32 437
Cuts at: 3140 3172 3464 3496 3515 3547 3739 3771 5482
Size: 32 292 32 19 32 192 32 1711
Cuts at: 5482 5514 5556 5588 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1711 826 437 292 241 192 135
42 32 32 32 32 32 32 32
32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2424 4185 4431 669
Size: 988 281 486 1761 246 3079
Fragments arranged by size:
3079 1761 988 486 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6841
HpaI GTT'AAC
Cuts at: 4431 4431
Size: 6841
KpnI G_GTAC'C
Cuts at: 4166 4166
Size: 6841
MfeI C'AATT_G
Cuts at: 4440 4440
Size: 6841
MluI A'CGCG_T
Cuts at: 6717 6717
Size: 6841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5955 6139 1305
Size: 4650 184 2007
Fragments arranged by size:
4650 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5019
Fragments arranged by size:
5019 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2109 2796 3339 4062 4799 5158 5317
Size: 648 943 687 543 723 737 359 159
Cuts at: 5317 518
Size: 2042
Fragments arranged by size:
2042 943 737 723 687 648 543 359
159
MspA1I CmG'CkG
Cuts at: 1606 2153 2815 3889 3949 4134 4257 4934 6082
Size: 547 662 1074 60 185 123 677 1148
Cuts at: 6082 6327 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1074 677 662 547 245 185
123 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5350
Fragments arranged by size:
5350 939 552
NdeI CA'TA_TG
Cuts at: 387 3220 3931 387
Size: 2833 711 3297
Fragments arranged by size:
3297 2833 711
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6841
NotI GC'GGCC_GC
Cuts at: 4288 4288
Size: 6841
NsiI A_TGCA'T
Cuts at: 3508 3556 3560 3508
Size: 48 4 6789
Fragments arranged by size:
6789 48 4
NspI r_CATG'y
Cuts at: 1132 1229 3154 3558 4199 5744 1132
Size: 97 1925 404 641 1545 2229
Fragments arranged by size:
2229 1925 1545 641 404 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6841
PciI A'CATG_T
Cuts at: 5740 5740
Size: 6841
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6841
PfoI T'CCnGG_A
Cuts at: 2897 3080 2897
Size: 183 6658
PmeI GTTT'AAAC
Cuts at: 4145 4145
Size: 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 4910 4942 6423 6455 2981
Size: 32 1897 32 1481 32 3367
Fragments arranged by size:
3367 1897 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2587
Size: 6841
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6841
PsiI TTA'TAA
Cuts at: 2138 4411 2138
Size: 2273 4568
PspOMI G'GGCC_C
Cuts at: 3969 3969
Size: 6841
PstI C_TGCA'G
Cuts at: 830 2091 3894 4026 4193 830
Size: 1261 1803 132 167 3478
Fragments arranged by size:
3478 1803 1261 167 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6841
PvuII CAG'CTG
Cuts at: 2153 2815 3889 3949 2153
Size: 662 1074 60 5045
Fragments arranged by size:
5045 1074 662 60
SalI G'TCGA_C
Cuts at: 1936 4183 1936
Size: 2247 4594
SanDI GG'GwC_CC
Cuts at: 2587 2587
Size: 6841
SbfI CC_TGCA'GG
Cuts at: 4193 4193
Size: 6841
ScaI AGT'ACT
Cuts at: 5089 6801 5089
Size: 1712 5129
SfcI C'TryA_G
Cuts at: 826 1045 2087 3890 4022 4189 5324 6005 6196
Size: 219 1042 1803 132 167 1135 681 191
Cuts at: 6196 826
Size: 1471
Fragments arranged by size:
1803 1471 1135 1042 681 219 191 167
132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6841
SmaI CCC'GGG
Cuts at: 1872 2907 4168 1872
Size: 1035 1261 4545
Fragments arranged by size:
4545 1261 1035
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4945 5846 6108 820
Size: 197 926 3002 901 262 1553
Fragments arranged by size:
3002 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4632 493
Size: 4139 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6841
SphI G_CATG'C
Cuts at: 1132 3558 4199 1132
Size: 2426 641 3774
Fragments arranged by size:
3774 2426 641
SrfI GCCC'GGGC
Cuts at: 2907 2907
Size: 6841
SspI AAT'ATT
Cuts at: 5 52 4040 4765 5
Size: 47 3988 725 2081
Fragments arranged by size:
3988 2081 725 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6841
StyI C'CwwG_G
Cuts at: 513 1065 2004 2941 3632 4280 513
Size: 552 939 937 691 648 3074
Fragments arranged by size:
3074 939 937 691 648 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2718 4868 4885 5038 5223 5668 325
Size: 1255 1138 2150 17 153 185 445 1498
Fragments arranged by size:
2150 1498 1255 1138 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5087 6799 96
Size: 274 80 33 51 1443 3110 1712 138
Fragments arranged by size:
3110 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2016 2048 3628 3660 6801 6833 2016
Size: 32 1580 32 3141 32 2024
Fragments arranged by size:
3141 2024 1580 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6841
XbaI T'CTAG_A
Cuts at: 4177 4177
Size: 6841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4064 4064
Size: 6841
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6841
XmaI C'CCGG_G
Cuts at: 1870 2905 4166 1870
Size: 1035 1261 4545
Fragments arranged by size:
4545 1261 1035
XmnI GAAnn'nnTTC
Cuts at: 4970 4970
Size: 6841
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4649 276
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SwaI