Restriction map for
pFN21AA1664
(Circular) (Six-base) MAPSORT of: pFN21AA1664.seq Check: 8117 from: 1 to: 7453
pFN21AA1664 7453 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3699 3862 3699
Size: 163 7290
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2267 5263 278
Size: 53 83 186 936 731 2996 2468
Fragments arranged by size:
2996 2468 936 731 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4796 1937
Size: 2859 4594
Acc65I G'GTAC_C
Cuts at: 4774 4774
Size: 7453
AclI AA'CG_TT
Cuts at: 1563 5580 5953 1563
Size: 4017 373 3063
Fragments arranged by size:
4017 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2612 2942 2957 3074 3453 4169 5516 6900
Size: 980 330 15 117 379 716 1347 1384
Cuts at: 6900 7168 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1347 980 716 379 330 268
117 15
AfeI AGC'GCT
Cuts at: 2125 2999 2125
Size: 874 6579
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7256
AflIII A'CryG_T
Cuts at: 1203 2670 3906 4035 4395 6352 7329 1203
Size: 1467 1236 129 360 1957 977 1327
Fragments arranged by size:
1957 1467 1327 1236 977 360 129
AgeI A'CCGG_T
Cuts at: 4137 6284 4137
Size: 2147 5306
AhdI GACnn_n'nnGTC
Cuts at: 6182 6182
Size: 7453
AleI CACnn'nnGTG
Cuts at: 1166 3776 1166
Size: 2610 4843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2648 3727 1184
Size: 1464 1079 4910
Fragments arranged by size:
4910 1464 1079
AlwNI CAG_nnn'CTG
Cuts at: 2285 3664 4342 4486 4534 4552 4596 5196 6768
Size: 1379 678 144 48 18 44 600 1572
Cuts at: 6768 2285
Size: 2970
Fragments arranged by size:
2970 1572 1379 678 600 144 48 44
18
ApaI G_GGCC'C
Cuts at: 2358 2358
Size: 7453
ApaLI G'TGCA_C
Cuts at: 3194 3707 3779 5510 6666 3194
Size: 513 72 1731 1156 3981
Fragments arranged by size:
3981 1731 1156 513 72
ApoI r'AATT_y
Cuts at: 1493 4010 4762 4988 5140 7249 1493
Size: 2517 752 226 152 2109 1697
Fragments arranged by size:
2517 2109 1697 752 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6007 160
Size: 872 4975 1606
Fragments arranged by size:
4975 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7453
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 2420 3242 4778 1870
Size: 73 445 32 822 1536 4545
Fragments arranged by size:
4545 1536 822 445 73 32
BamHI G'GATC_C
Cuts at: 3615 4660 4783 5150 3615
Size: 1045 123 367 5918
Fragments arranged by size:
5918 1045 367 123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2984 3491 3995 4140
Size: 325 217 627 323 874 507 504 145
Cuts at: 4140 4560 4774 6229 618
Size: 420 214 1455 1842
Fragments arranged by size:
1842 1455 874 627 507 504 420 325
323 217 214 145
BanII G_rGCy'C
Cuts at: 1391 2064 2154 2358 3470 3609 3645 4772 1391
Size: 673 90 204 1112 139 36 1127 4072
Fragments arranged by size:
4072 1127 1112 673 204 139 90 36
BbeI G_GCGC'C
Cuts at: 2114 2988 4564 2114
Size: 874 1576 5003
Fragments arranged by size:
5003 1576 874
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2486 3381 3423 4331 928
Size: 971 587 895 42 908 4050
Fragments arranged by size:
4050 971 908 895 587 42
BbvCI CC'TCA_GC
Cuts at: 2951 3892 2951
Size: 941 6512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5644 5678 1903
Size: 34 3707 34 3678
Fragments arranged by size:
3707 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5345 6555 5345
Size: 1210 6243
BclI T'GATC_A
Cuts at: 1548 3105 3399 1548
Size: 1557 294 5602
Fragments arranged by size:
5602 1557 294
BfrBI ATG'CAT
Cuts at: 4285 4285
Size: 7453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 2534 6064
Size: 107 122 71 631 605 362 500 3530
Cuts at: 6064 136
Size: 1525
Fragments arranged by size:
3530 1525 631 605 500 362 122 107
71
BglII A'GATC_T
Cuts at: 7448 7448
Size: 7453
BlpI GC'TnA_GC
Cuts at: 3469 4870 3469
Size: 1401 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2358 2530 3169 3198 3265 3507 3711
Size: 388 180 172 639 29 67 242 204
Cuts at: 3711 3783 4742 5514 6670 1790
Size: 72 959 772 1156 2573
Fragments arranged by size:
2573 1156 959 772 639 388 242 204
180 172 72 67 29
BmgBI CAC'GTC
Cuts at: 1318 2133 1318
Size: 815 6638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4714 5184 6142
Size: 416 53 83 399 24 3297 470 958
Cuts at: 6142 442
Size: 1753
Fragments arranged by size:
3297 1753 958 470 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2401 2353
Size: 48 7405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2506 2831 2978 3484 3595 3641 3698 3937
Size: 1267 325 147 506 111 46 57 239
Cuts at: 3937 3946 4556 4592 6113 1239
Size: 9 610 36 1521 2579
Fragments arranged by size:
2579 1521 1267 610 506 325 239 147
111 57 46 36 9
Bpu10I CC'TnA_GC
Cuts at: 2338 2951 3892 2338
Size: 613 941 5899
Fragments arranged by size:
5899 941 613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2750 3008 5578 6443 6741 2558
Size: 192 258 2570 865 298 3270
Fragments arranged by size:
3270 2570 865 298 258 192
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3812 6116 882
Size: 617 2313 2304 2219
Fragments arranged by size:
2313 2304 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2673 2958 3907 4036 4237 5244 493
Size: 711 1469 285 949 129 201 1007 2702
Fragments arranged by size:
2702 1469 1007 949 711 285 201 129
BsaBI GATnn'nnATC
Cuts at: 3947 7447 3947
Size: 3500 3953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 2264 2985 4561
Size: 53 83 186 936 578 153 721 1576
Cuts at: 4561 5260 5642 6277 275
Size: 699 382 635 1451
Fragments arranged by size:
1576 1451 936 721 699 635 578 382
186 153 83 53
BsaWI w'CCGG_w
Cuts at: 2182 4137 5886 6284 6558 6705 7116 2182
Size: 1955 1749 398 274 147 411 2519
Fragments arranged by size:
2519 1955 1749 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3719 3749 3896 3926 4382
Size: 30 165 30 2316 30 147 30 456
Cuts at: 4382 4412 1178
Size: 30 4219
Fragments arranged by size:
4219 2316 456 165 147 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2475 4454 4553 1186
Size: 1289 1979 99 4086
Fragments arranged by size:
4086 1979 1289 99
BseYI C'CCAG_C
Cuts at: 2160 2166 2285 2351 2620 2890 3811 4295 4304
Size: 6 119 66 269 270 921 484 9
Cuts at: 4304 6656 7152 2160
Size: 2352 496 2461
Fragments arranged by size:
2461 2352 921 496 484 270 269 119
66 9 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2894 3697 1163
Size: 573 1158 803 4919
Fragments arranged by size:
4919 1158 803 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 2321 3275 3601 4903 5664
Size: 275 186 49 271 954 326 1302 761
Cuts at: 5664 6692 1540
Size: 1028 2301
Fragments arranged by size:
2301 1302 1028 954 761 326 275 271
186 49
BsiHKAI G_wGCw'C
Cuts at: 3198 3470 3609 3711 3783 4052 4274 4437 5514
Size: 272 139 102 72 269 222 163 1077
Cuts at: 5514 5599 6670 3198
Size: 85 1071 3981
Fragments arranged by size:
3981 1077 1071 272 269 222 163 139
102 85 72
BsmI GAATG_Cn'
Cuts at: 3540 4964 5057 3540
Size: 1424 93 5936
Fragments arranged by size:
5936 1424 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2252 2252
Size: 7453
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2154 2178 2358 2530 3169 3198
Size: 399 274 90 24 180 172 639 29
Cuts at: 3198 3265 3470 3507 3609 3645 3711 3783 4052
Size: 67 205 37 102 36 66 72 269
Cuts at: 4052 4274 4437 4742 4772 5514 5599 6670 1391
Size: 222 163 305 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 639 399 305 274 269
222 205 180 172 163 102 90 85
72 67 66 37 36 30 29 24
BspEI T'CCGG_A
Cuts at: 7116 7116
Size: 7453
BspHI T'CATG_A
Cuts at: 3582 5342 7072 3582
Size: 1760 1730 3963
Fragments arranged by size:
3963 1760 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3699 3862 4808 844
Size: 2855 163 946 3489
Fragments arranged by size:
3489 2855 946 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2052 2188 2383 3070 3247 5340 1394
Size: 429 229 136 195 687 177 2093 3507
Fragments arranged by size:
3507 2093 687 429 229 195 177 136
BsrDI GCAATG_nn'
Cuts at: 65 1248 2632 2968 5948 6122 65
Size: 1183 1384 336 2980 174 1396
Fragments arranged by size:
2980 1396 1384 1183 336 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2787 3270 3274 3625 4137 4563 6097
Size: 110 977 483 4 351 512 426 1534
Cuts at: 6097 6284 1700
Size: 187 2869
Fragments arranged by size:
2869 1534 977 512 483 426 351 187
110 4
BsrGI T'GTAC_A
Cuts at: 96 4689 96
Size: 4593 2860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7453
BssSI C'ACGA_G
Cuts at: 3500 4249 4436 5513 6525 3500
Size: 749 187 1077 1012 4428
Fragments arranged by size:
4428 1077 1012 749 187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3193 4596 794
Size: 2399 1403 3651
Fragments arranged by size:
3651 2399 1403
BstBI TT'CG_AA
Cuts at: 6257 6257
Size: 7453
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7453
BstXI CCAn_nnnn'nTGG
Cuts at: 3724 4022 3724
Size: 298 7155
BstYI r'GATC_y
Cuts at: 1972 3027 3615 4660 4783 5150 5535 5552 7004
Size: 1055 588 1045 123 367 385 17 1452
Cuts at: 7004 7090 7102 7448 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1055 1045 588 385 367 346
123 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2056 2677 3369 3456 513
Size: 552 939 52 621 692 87 4510
Fragments arranged by size:
4510 939 692 621 552 87 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2956 3772 487
Size: 683 56 1730 816 4168
Fragments arranged by size:
4168 1730 816 683 56
BtsI GCAGTG_nn'
Cuts at: 2238 2801 3167 3709 3711 4969 5763 5783 2238
Size: 563 366 542 2 1258 794 20 3908
Fragments arranged by size:
3908 1258 794 563 542 366 20 2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3438 3473 529
Size: 35 2874 35 4509
Fragments arranged by size:
4509 2874 35 35
DraI TTT'AAA
Cuts at: 4757 4757
Size: 7453
DraIII CAC_nnn'GTG
Cuts at: 3907 4036 3907
Size: 129 7324
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6460 809
Size: 507 5144 1802
Fragments arranged by size:
5144 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2318 2817
Size: 54 1425 50 275 18 217 271 499
Cuts at: 2817 3268 3272 3276 3598 3744 4900 5789 7418
Size: 451 4 4 322 146 1156 889 1629
Cuts at: 7418 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 499 451 322 275
271 217 146 54 50 43 18 4
4
EagI C'GGCC_G
Cuts at: 1537 1812 2047 2318 3272 3598 4900 1537
Size: 275 235 271 954 326 1302 4090
Fragments arranged by size:
4090 1302 954 326 275 271 235
EarI CTCTTCn'nnn_
Cuts at: 1353 2463 3086 4040 4605 4646 5383 1353
Size: 1110 623 954 565 41 737 3423
Fragments arranged by size:
3423 1110 954 737 623 565 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4637 6040 6414 6560 387
Size: 1064 138 3048 1403 374 146 1280
Fragments arranged by size:
3048 1403 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3468 3607 3468
Size: 139 7314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2506 2612 2831 2942 2957 2978 3074
Size: 393 874 106 219 111 15 21 96
Cuts at: 3074 3453 3484 3595 3641 3698 3937 3946 4169
Size: 379 31 111 46 57 239 9 223
Cuts at: 4169 4556 4592 5516 6113 6900 7168 1239
Size: 387 36 924 597 787 268 1524
Fragments arranged by size:
1524 924 874 787 597 393 387 379
268 239 223 219 111 111 106 96
57 46 36 31 21 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3935 3935
Size: 7453
EcoO109I rG'GnC_Cy
Cuts at: 2355 3673 2355
Size: 1318 6135
EcoRI G'AATT_C
Cuts at: 4010 4762 5140 4010
Size: 752 378 6323
Fragments arranged by size:
6323 752 378
FseI GG_CCGG'CC
Cuts at: 3274 3278 3274
Size: 4 7449
FspI TGC'GCA
Cuts at: 3900 5959 3900
Size: 2059 5394
HaeII r_GCGC'y
Cuts at: 1678 2114 2127 2608 2988 3001 4454 4552 4564
Size: 436 13 481 380 13 1453 98 12
Cuts at: 4564 6600 1678
Size: 2036 2531
Fragments arranged by size:
2531 2036 1453 481 436 380 98 13
13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2414 2446 2572
Size: 32 135 32 677 32 133 32 126
Cuts at: 2572 2604 2833 2865 3133 3165 3368 3400 3452
Size: 32 229 32 268 32 203 32 52
Cuts at: 3452 3479 3484 3511 3632 3664 4645 4677 6094
Size: 27 5 27 121 32 981 32 1417
Cuts at: 6094 6126 6168 6200 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 981 677 268 229 203 135
133 126 121 52 42 32 32 32
32 32 32 32 32 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4797 5043 669
Size: 988 281 2859 246 3079
Fragments arranged by size:
3079 2859 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7453
HpaI GTT'AAC
Cuts at: 5043 5043
Size: 7453
KasI G'GCGC_C
Cuts at: 2110 2984 4560 2110
Size: 874 1576 5003
Fragments arranged by size:
5003 1576 874
KpnI G_GTAC'C
Cuts at: 4778 4778
Size: 7453
MfeI C'AATT_G
Cuts at: 5052 5052
Size: 7453
MluI A'CGCG_T
Cuts at: 7329 7329
Size: 7453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 3131 6567 6751 1305
Size: 888 938 3436 184 2007
Fragments arranged by size:
3436 2007 938 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2819 10
Size: 54 1425 343 987 4644
Fragments arranged by size:
4644 1425 987 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2856 3395 3626 3776 4073 5411 5770
Size: 648 1690 539 231 150 297 1338 359
Cuts at: 5770 5929 518
Size: 159 2042
Fragments arranged by size:
2042 1690 1338 648 539 359 297 231
159 150
MspA1I CmG'CkG
Cuts at: 1606 2058 2197 2610 3163 3220 3661 3896 4147
Size: 452 139 413 553 57 441 235 251
Cuts at: 4147 4375 4483 4531 4596 4666 4869 5546 6694
Size: 228 108 48 65 70 203 677 1148
Cuts at: 6694 6939 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 553 452 441 413 251
245 235 228 203 139 108 70 65
57 48
NaeI GCC'GGC
Cuts at: 3272 3276 4565 3272
Size: 4 1289 6160
Fragments arranged by size:
6160 1289 4
NarI GG'CG_CC
Cuts at: 2111 2985 4561 2111
Size: 874 1576 5003
Fragments arranged by size:
5003 1576 874
NcoI C'CATG_G
Cuts at: 513 1065 2004 3369 513
Size: 552 939 1365 4597
Fragments arranged by size:
4597 1365 939 552
NdeI CA'TA_TG
Cuts at: 387 4734 387
Size: 4347 3106
NgoMIV G'CCGG_C
Cuts at: 3270 3274 4563 3270
Size: 4 1289 6160
Fragments arranged by size:
6160 1289 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7453
NotI GC'GGCC_GC
Cuts at: 2047 4900 2047
Size: 2853 4600
NsiI A_TGCA'T
Cuts at: 4287 4287
Size: 7453
NspI r_CATG'y
Cuts at: 1132 1229 4399 4811 6356 1132
Size: 97 3170 412 1545 2229
Fragments arranged by size:
3170 2229 1545 412 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7453
PciI A'CATG_T
Cuts at: 4395 6352 4395
Size: 1957 5496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2285 3694 1108
Size: 1177 1409 4867
Fragments arranged by size:
4867 1409 1177
PfoI T'CCnGG_A
Cuts at: 3611 3611
Size: 7453
PmeI GTTT'AAAC
Cuts at: 4757 4757
Size: 7453
PmlI CAC'GTG
Cuts at: 2673 2958 2673
Size: 285 7168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5522 5554 7035 7067 5522
Size: 32 1481 32 5908
Fragments arranged by size:
5908 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3673 3673
Size: 7453
PshAI GACnn'nnGTC
Cuts at: 1613 2197 2422 1613
Size: 584 225 6644
Fragments arranged by size:
6644 584 225
PsiI TTA'TAA
Cuts at: 5023 5023
Size: 7453
PspOMI G'GGCC_C
Cuts at: 2354 2354
Size: 7453
PspXI vC'TCGA_Gb
Cuts at: 3242 3242
Size: 7453
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4385 4417 4385
Size: 32 7421
PstI C_TGCA'G
Cuts at: 830 2310 3119 3174 4601 4805 830
Size: 1480 809 55 1427 204 3478
Fragments arranged by size:
3478 1480 1427 809 204 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7453
PvuII CAG'CTG
Cuts at: 3220 3896 4147 4596 3220
Size: 676 251 449 6077
Fragments arranged by size:
6077 676 449 251
SacI G_AGCT'C
Cuts at: 3470 3609 3470
Size: 139 7314
SacII CC_GC'GG
Cuts at: 2059 2059
Size: 7453
SalI G'TCGA_C
Cuts at: 1936 4795 1936
Size: 2859 4594
SapI GCTCTTCn'nnn_
Cuts at: 4040 4040
Size: 7453
SbfI CC_TGCA'GG
Cuts at: 3119 4805 3119
Size: 1686 5767
ScaI AGT'ACT
Cuts at: 3359 5701 7413 3359
Size: 2342 1712 3399
Fragments arranged by size:
3399 2342 1712
SfcI C'TryA_G
Cuts at: 826 1045 2306 2644 3115 3170 3559 4162 4597
Size: 219 1261 338 471 55 389 603 435
Cuts at: 4597 4801 5936 6617 6808 826
Size: 204 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 681 603 471 435 389
338 219 204 191 55
SfoI GGC'GCC
Cuts at: 2112 2986 4562 2112
Size: 874 1576 5003
Fragments arranged by size:
5003 1576 874
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7453
SgrAI Cr'CCGG_yG
Cuts at: 1700 4563 1700
Size: 2863 4590
SmaI CCC'GGG
Cuts at: 1872 2390 4780 1872
Size: 518 2390 4545
Fragments arranged by size:
4545 2390 518
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 2765 3023 3242 5557 6458
Size: 197 926 630 192 258 219 2315 901
Cuts at: 6458 6720 820
Size: 262 1553
Fragments arranged by size:
2315 1553 926 901 630 262 258 219
197 192
SnaBI TAC'GTA
Cuts at: 493 5244 493
Size: 4751 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7453
SphI G_CATG'C
Cuts at: 1132 4811 1132
Size: 3679 3774
SspI AAT'ATT
Cuts at: 5 52 5377 5
Size: 47 5325 2081
Fragments arranged by size:
5325 2081 47
StuI AGG'CCT
Cuts at: 1517 4577 1517
Size: 3060 4393
StyI C'CwwG_G
Cuts at: 513 1065 2004 2820 2826 3369 3411 3507 4275
Size: 552 939 816 6 543 42 96 768
Cuts at: 4275 4713 4892 513
Size: 438 179 3074
Fragments arranged by size:
3074 939 816 768 552 543 438 179
96 42 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5480 5497 5650 5835 6280 325
Size: 1255 3900 17 153 185 445 1498
Fragments arranged by size:
3900 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3357 3481 4398
Size: 274 80 33 51 1443 1380 124 917
Cuts at: 4398 4689 5699 7411 96
Size: 291 1010 1712 138
Fragments arranged by size:
1712 1443 1380 1010 917 291 274 138
124 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3719 3751 7413 7445 3719
Size: 32 3662 32 3727
Fragments arranged by size:
3727 3662 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2263 4342 1316
Size: 947 2079 4427
Fragments arranged by size:
4427 2079 947
XbaI T'CTAG_A
Cuts at: 4789 4789
Size: 7453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2675 2779 3217 3263 2530
Size: 145 104 438 46 6720
Fragments arranged by size:
6720 438 145 104 46
XhoI C'TCGA_G
Cuts at: 1943 3242 1943
Size: 1299 6154
XmaI C'CCGG_G
Cuts at: 1870 2388 4778 1870
Size: 518 2390 4545
Fragments arranged by size:
4545 2390 518
XmnI GAAnn'nnTTC
Cuts at: 5582 5582
Size: 7453
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2265 5261 276
Size: 53 83 186 936 731 2996 2468
Fragments arranged by size:
2996 2468 936 731 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstZ17I Bsu36I EcoRV
FalI FspAI NruI PacI RsrII SanDI SexAI SfiI
SrfI SwaI