Restriction map for
pFN21AA1643
(Circular) (Six-base) MAPSORT of: pFN21AA1643.seq Check: 1207 from: 1 to: 7363
pFN21AA1643 7363 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4576 4576
Size: 7363
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2499 5173 278
Size: 53 83 186 936 963 2674 2468
Fragments arranged by size:
2674 2468 963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3160 4706 1937
Size: 1223 1546 4594
Fragments arranged by size:
4594 1546 1223
Acc65I G'GTAC_C
Cuts at: 2628 4684 2628
Size: 2056 5307
AclI AA'CG_TT
Cuts at: 1563 5490 5863 1563
Size: 3927 373 3063
Fragments arranged by size:
3927 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5426 6810 7078 1632
Size: 3794 1384 268 1917
Fragments arranged by size:
3794 1917 1384 268
AfeI AGC'GCT
Cuts at: 2481 2481
Size: 7363
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7166
AflIII A'CryG_T
Cuts at: 1203 2757 2784 4620 6262 7239 1203
Size: 1554 27 1836 1642 977 1327
Fragments arranged by size:
1836 1642 1554 1327 977 27
AgeI A'CCGG_T
Cuts at: 6194 6194
Size: 7363
AhdI GACnn_n'nnGTC
Cuts at: 3067 6092 3067
Size: 3025 4338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2076 2387 1184
Size: 892 311 6160
Fragments arranged by size:
6160 892 311
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 7331
AlwNI CAG_nnn'CTG
Cuts at: 2440 2821 3025 3288 3435 4039 4336 5106 6678
Size: 381 204 263 147 604 297 770 1572
Cuts at: 6678 2440
Size: 3125
Fragments arranged by size:
3125 1572 770 604 381 297 263 204
147
ApaI G_GGCC'C
Cuts at: 2617 3820 2617
Size: 1203 6160
ApaLI G'TGCA_C
Cuts at: 2146 4362 4606 5420 6576 2146
Size: 2216 244 814 1156 2933
Fragments arranged by size:
2933 2216 1156 814 244
ApoI r'AATT_y
Cuts at: 1493 4672 4898 5050 7159 1493
Size: 3179 226 152 2109 1697
Fragments arranged by size:
3179 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5917 160
Size: 872 4885 1606
Fragments arranged by size:
4885 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7363
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 3389 3760 4688 1870
Size: 73 1305 141 371 928 4545
Fragments arranged by size:
4545 1305 928 371 141 73
BamHI G'GATC_C
Cuts at: 2047 4693 5060 2047
Size: 2646 367 4350
Fragments arranged by size:
4350 2646 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2420 2539 2628 2920
Size: 325 217 627 525 108 119 89 292
Cuts at: 2920 3425 3776 3838 4027 4444 4684 6139 618
Size: 505 351 62 189 417 240 1455 1842
Fragments arranged by size:
1842 1455 627 525 505 417 351 325
292 240 217 189 119 108 89 62
BanII G_rGCy'C
Cuts at: 1391 2520 2617 3621 3820 3879 4013 4049 4682
Size: 1129 97 1004 199 59 134 36 633
Cuts at: 4682 1391
Size: 4072
Fragments arranged by size:
4072 1129 1004 633 199 134 97 59
36
BbeI G_GCGC'C
Cuts at: 2424 2924 3842 4031 2424
Size: 500 918 189 5756
Fragments arranged by size:
5756 918 500 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2760 4434 928
Size: 971 861 1674 3857
Fragments arranged by size:
3857 1674 971 861
BbvCI CC'TCA_GC
Cuts at: 2483 2483
Size: 7363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 5554 5588 1903
Size: 34 1991 34 1592 34 3678
Fragments arranged by size:
3678 1991 1592 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5255 6465 5255
Size: 1210 6153
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7363
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3423 3765 4130
Size: 107 122 71 631 605 1751 342 365
Cuts at: 4130 5974 136
Size: 1844 1525
Fragments arranged by size:
1844 1751 1525 631 605 365 342 122
107 71
BglII A'GATC_T
Cuts at: 4533 7358 4533
Size: 2825 4538
BlpI GC'TnA_GC
Cuts at: 2519 3035 3656 3796 4780 2519
Size: 516 621 140 984 5102
Fragments arranged by size:
5102 984 621 516 140
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2617 3404 3428 3820 4366 4610 5424
Size: 360 467 787 24 392 546 244 814
Cuts at: 5424 6580 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 814 787 546 467 392 360
244 24
BmgBI CAC'GTC
Cuts at: 1318 2413 2875 2899 3217 3730 1318
Size: 1095 462 24 318 513 4951
Fragments arranged by size:
4951 1095 513 462 318 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 5094 6052
Size: 416 53 83 399 24 2255 1422 958
Cuts at: 6052 442
Size: 1753
Fragments arranged by size:
2255 1753 1422 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3628 3088
Size: 540 6823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2426 3047 3138 4033 6023 1239
Size: 1139 48 621 91 895 1990 2579
Fragments arranged by size:
2579 1990 1139 895 621 91 48
Bpu10I CC'TnA_GC
Cuts at: 2483 2967 2483
Size: 484 6879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3394 5488 6353 6651 3394
Size: 2094 865 298 4106
Fragments arranged by size:
4106 2094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 6026 882
Size: 617 2871 1656 2219
Fragments arranged by size:
2871 2219 1656 617
BsaAI yAC'GTr
Cuts at: 493 1204 2569 5154 493
Size: 711 1365 2585 2702
Fragments arranged by size:
2702 2585 1365 711
BsaBI GATnn'nnATC
Cuts at: 7357 7357
Size: 7363
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2421 2496 2921 3768
Size: 53 83 186 936 888 75 425 847
Cuts at: 3768 3839 4028 5170 5552 6187 275
Size: 71 189 1142 382 635 1451
Fragments arranged by size:
1451 1142 936 888 847 635 425 382
189 186 83 75 71 53
BsaWI w'CCGG_w
Cuts at: 3519 4149 5796 6194 6468 6615 7026 3519
Size: 630 1647 398 274 147 411 3856
Fragments arranged by size:
3856 1647 630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3185 3215 4367 4397 1178
Size: 30 165 30 1782 30 1152 30 4144
Fragments arranged by size:
4144 1782 1152 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2285 2843 2846 3203 3206 3380 3848
Size: 913 186 558 3 357 3 174 468
Cuts at: 3848 4066 4543 1186
Size: 218 477 4006
Fragments arranged by size:
4006 913 558 477 468 357 218 186
174 3 3
BseYI C'CCAG_C
Cuts at: 2139 3106 3256 3471 3959 4004 4064 4105 6566
Size: 967 150 215 488 45 60 41 2461
Cuts at: 6566 7062 2139
Size: 496 2440
Fragments arranged by size:
2461 2440 967 496 488 215 150 60
45 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2591 2822 2921 3443 3775 4453 1163
Size: 573 855 231 99 522 332 678 4073
Fragments arranged by size:
4073 855 678 573 522 332 231 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3594 4813 5574 6602 1540
Size: 275 186 1593 1219 761 1028 2301
Fragments arranged by size:
2301 1593 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2150 2520 2592 4366 4610 5424 5509 6580 2150
Size: 370 72 1774 244 814 85 1071 2933
Fragments arranged by size:
2933 1774 1071 814 370 244 85 72
BsiWI C'GTAC_G
Cuts at: 3783 3783
Size: 7363
BsmI GAATG_Cn'
Cuts at: 3685 4874 4967 3685
Size: 1189 93 6081
Fragments arranged by size:
6081 1189 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2533 2594 3491 2533
Size: 61 897 6405
Fragments arranged by size:
6405 897 61
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2520 2592 2617 3404 3428 3621
Size: 399 360 370 72 25 787 24 193
Cuts at: 3621 3820 3879 4013 4049 4366 4610 4682 5424
Size: 199 59 134 36 317 244 72 742
Cuts at: 5424 5509 6580 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 787 742 399 370 360 317
244 199 193 134 85 72 72 59
36 25 24
BspEI T'CCGG_A
Cuts at: 7026 7026
Size: 7363
BspHI T'CATG_A
Cuts at: 2343 5252 6982 2343
Size: 2909 1730 2724
Fragments arranged by size:
2909 2724 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3959 4576 4718 844
Size: 3115 617 142 3489
Fragments arranged by size:
3489 3115 617 142
BsrBI CCG'CTC
Cuts at: 1394 1823 2172 3125 3332 5250 1394
Size: 429 349 953 207 1918 3507
Fragments arranged by size:
3507 1918 953 429 349 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 3090 5858 6032 65
Size: 1183 1842 2768 174 1396
Fragments arranged by size:
2768 1842 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2294 2330 2662 3791 4594 4655 6007
Size: 110 484 36 332 1129 803 61 1352
Cuts at: 6007 6194 1700
Size: 187 2869
Fragments arranged by size:
2869 1352 1129 803 484 332 187 110
61 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7363
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7363
BssSI C'ACGA_G
Cuts at: 2708 4022 5423 6435 2708
Size: 1314 1401 1012 3636
Fragments arranged by size:
3636 1401 1314 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4476 4614 794
Size: 3682 138 3543
Fragments arranged by size:
3682 3543 138
BstBI TT'CG_AA
Cuts at: 6167 6167
Size: 7363
BstEII G'GTnAC_C
Cuts at: 1184 4324 1184
Size: 3140 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 3273 3580 3273
Size: 307 7056
BstYI r'GATC_y
Cuts at: 1972 2047 2274 4146 4533 4693 5060 5445 5462
Size: 75 227 1872 387 160 367 385 17
Cuts at: 5462 6914 7000 7012 7358 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1872 1452 387 385 367 346 227
160 86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 2268 2949 3916 4032 2268
Size: 681 967 116 5599
Fragments arranged by size:
5599 967 681 116
BtgI C'CryG_G
Cuts at: 513 1065 2103 2535 3546 3621 4162 513
Size: 552 1038 432 1011 75 541 3714
Fragments arranged by size:
3714 1038 1011 552 541 432 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4428 487
Size: 683 56 3202 3422
Fragments arranged by size:
3422 3202 683 56
BtsI GCAGTG_nn'
Cuts at: 3187 4513 4612 4879 5673 5693 3187
Size: 1326 99 267 794 20 4857
Fragments arranged by size:
4857 1326 794 267 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7363
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7328
DraI TTT'AAA
Cuts at: 4667 4667
Size: 7363
DraIII CAC_nnn'GTG
Cuts at: 4476 4476
Size: 7363
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3590 6370 809
Size: 507 2274 2780 1802
Fragments arranged by size:
2780 2274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2292 2296
Size: 54 1425 50 275 18 270 192 4
Cuts at: 2296 2325 2367 2382 2532 2691 3240 3624 4281
Size: 29 42 15 150 159 549 384 657
Cuts at: 4281 4810 5699 7328 8
Size: 529 889 1629 43
Fragments arranged by size:
1629 1425 889 657 549 529 384 275
270 192 159 150 54 50 43 42
29 18 15 4
EagI C'GGCC_G
Cuts at: 1537 1812 4810 1537
Size: 275 2998 4090
Fragments arranged by size:
4090 2998 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2597 5293 1353
Size: 1244 2696 3423
Fragments arranged by size:
3423 2696 1244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2143 3342 4490 5950 6324 6470
Size: 1064 138 554 1199 1148 1460 374 146
Cuts at: 6470 387
Size: 1280
Fragments arranged by size:
1460 1280 1199 1148 1064 554 374 146
138
EcoICRI GAG'CTC
Cuts at: 2518 2518
Size: 7363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2426 3047 3138 4033 5426 6023
Size: 393 746 48 621 91 895 1393 597
Cuts at: 6023 6810 7078 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1393 895 787 746 621 597 393
268 91 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2636 2636
Size: 7363
EcoO109I rG'GnC_Cy
Cuts at: 2134 2446 2613 2614 2906 3816 3817 4036 4068
Size: 312 167 1 292 910 1 219 32
Cuts at: 4068 2134
Size: 5429
Fragments arranged by size:
5429 910 312 292 219 167 32 1
1
EcoRI G'AATT_C
Cuts at: 4672 5050 4672
Size: 378 6985
FseI GG_CCGG'CC
Cuts at: 2298 4659 2298
Size: 2361 5002
FspI TGC'GCA
Cuts at: 5869 5869
Size: 7363
HaeII r_GCGC'y
Cuts at: 1678 2424 2441 2483 2924 3842 4031 6510 1678
Size: 746 17 42 441 918 189 2479 2531
Fragments arranged by size:
2531 2479 918 746 441 189 42 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 3053 3085 4131
Size: 32 135 32 460 32 989 32 1046
Cuts at: 4131 4163 4367 4399 6004 6036 6078 6110 1373
Size: 32 204 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 1046 989 460 204 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4707 4953 669
Size: 988 281 2769 246 3079
Fragments arranged by size:
3079 2769 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3653 748
Size: 2905 4458
HpaI GTT'AAC
Cuts at: 4953 4953
Size: 7363
KasI G'GCGC_C
Cuts at: 2420 2920 3838 4027 2420
Size: 500 918 189 5756
Fragments arranged by size:
5756 918 500 189
KpnI G_GTAC'C
Cuts at: 2632 4688 2632
Size: 2056 5307
MfeI C'AATT_G
Cuts at: 4962 4962
Size: 7363
MluI A'CGCG_T
Cuts at: 7239 7239
Size: 7363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 3888 3918 6477 6661 1305
Size: 720 1863 30 2559 184 2007
Fragments arranged by size:
2559 2007 1863 720 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 2384 2693 3242 4283
Size: 54 1425 343 537 15 309 549 1041
Cuts at: 4283 10
Size: 3090
Fragments arranged by size:
3090 1425 1041 549 537 343 309 54
15
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 2369 3271 5321 5680 5839 518
Size: 648 1176 27 902 2050 359 159 2042
Fragments arranged by size:
2050 2042 1176 902 648 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 2056 2105 2143 2302 2380 2912 3260 3441
Size: 450 49 38 159 78 532 348 181
Cuts at: 3441 4064 4222 4524 4570 4779 5456 6604 6849
Size: 623 158 302 46 209 677 1148 245
Cuts at: 6849 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 623 532 450 348 302
245 209 181 159 158 78 49 46
38
NaeI GCC'GGC
Cuts at: 2296 2332 2664 3793 4657 2296
Size: 36 332 1129 864 5002
Fragments arranged by size:
5002 1129 864 332 36
NarI GG'CG_CC
Cuts at: 2421 2921 3839 4028 2421
Size: 500 918 189 5756
Fragments arranged by size:
5756 918 500 189
NcoI C'CATG_G
Cuts at: 513 1065 2535 513
Size: 552 1470 5341
Fragments arranged by size:
5341 1470 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7363
NgoMIV G'CCGG_C
Cuts at: 2294 2330 2662 3791 4655 2294
Size: 36 332 1129 864 5002
Fragments arranged by size:
5002 1129 864 332 36
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7363
NotI GC'GGCC_GC
Cuts at: 4810 4810
Size: 7363
NspI r_CATG'y
Cuts at: 1132 1229 2761 4004 4121 4624 4721 6266 1132
Size: 97 1532 1243 117 503 97 1545 2229
Fragments arranged by size:
2229 1545 1532 1243 503 117 97 97
PasI CC'CwG_GG
Cuts at: 1348 2556 1348
Size: 1208 6155
PciI A'CATG_T
Cuts at: 2757 4620 6262 2757
Size: 1863 1642 3858
Fragments arranged by size:
3858 1863 1642
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 2875 3025 1108
Size: 1104 663 150 5446
Fragments arranged by size:
5446 1104 663 150
PmeI GTTT'AAAC
Cuts at: 4667 4667
Size: 7363
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2841 2873 3185 3217 5432 5464 6945 6977 2841
Size: 32 312 32 2215 32 1481 32 3227
Fragments arranged by size:
3227 2215 1481 312 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2446 2906 4068 2134
Size: 312 460 1162 5429
Fragments arranged by size:
5429 1162 460 312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7363
PsiI TTA'TAA
Cuts at: 4933 4933
Size: 7363
PspOMI G'GGCC_C
Cuts at: 2613 3816 2613
Size: 1203 6160
PstI C_TGCA'G
Cuts at: 830 2817 3440 4227 4715 830
Size: 1987 623 787 488 3478
Fragments arranged by size:
3478 1987 787 623 488
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7363
PvuII CAG'CTG
Cuts at: 2056 2302 3260 3441 4064 4222 4524 2056
Size: 246 958 181 623 158 302 4895
Fragments arranged by size:
4895 958 623 302 246 181 158
RsrII CG'GwC_CG
Cuts at: 4597 4597
Size: 7363
SacI G_AGCT'C
Cuts at: 2520 2520
Size: 7363
SacII CC_GC'GG
Cuts at: 2106 2106
Size: 7363
SalI G'TCGA_C
Cuts at: 1936 4705 1936
Size: 2769 4594
SanDI GG'GwC_CC
Cuts at: 2906 2906
Size: 7363
SapI GCTCTTCn'nnn_
Cuts at: 2597 2597
Size: 7363
SbfI CC_TGCA'GG
Cuts at: 4715 4715
Size: 7363
ScaI AGT'ACT
Cuts at: 2564 5611 7323 2564
Size: 3047 1712 2604
Fragments arranged by size:
3047 2604 1712
SexAI A'CCwGG_T
Cuts at: 2094 2094
Size: 7363
SfcI C'TryA_G
Cuts at: 826 1045 2813 3436 4223 4642 4711 5846 6527
Size: 219 1768 623 787 419 69 1135 681
Cuts at: 6527 6718 826
Size: 191 1471
Fragments arranged by size:
1768 1471 1135 787 681 623 419 219
191 69
SfoI GGC'GCC
Cuts at: 2422 2922 3840 4029 2422
Size: 500 918 189 5756
Fragments arranged by size:
5756 918 500 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7363
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7363
SmaI CCC'GGG
Cuts at: 1872 3762 4690 1872
Size: 1890 928 4545
Fragments arranged by size:
4545 1890 928
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3373 3389 5467 6368 6630 820
Size: 197 926 1430 16 2078 901 262 1553
Fragments arranged by size:
2078 1553 1430 926 901 262 197 16
SnaBI TAC'GTA
Cuts at: 493 5154 493
Size: 4661 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7363
SphI G_CATG'C
Cuts at: 1132 4004 4121 4721 1132
Size: 2872 117 600 3774
Fragments arranged by size:
3774 2872 600 117
SspI AAT'ATT
Cuts at: 5 52 5287 5
Size: 47 5235 2081
Fragments arranged by size:
5235 2081 47
StuI AGG'CCT
Cuts at: 1517 3149 4457 1517
Size: 1632 1308 4423
Fragments arranged by size:
4423 1632 1308
StyI C'CwwG_G
Cuts at: 513 1065 2385 2535 3801 4350 4802 513
Size: 552 1320 150 1266 549 452 3074
Fragments arranged by size:
3074 1320 1266 552 549 452 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3261 3470 3614 4343 4432 4625 5390
Size: 1255 1681 209 144 729 89 193 765
Cuts at: 5390 5407 5560 5745 6190 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1681 1498 1255 765 729 445 209 193
185 153 144 89 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 5609 7321
Size: 274 80 33 51 1443 585 3047 1712
Cuts at: 7321 96
Size: 138
Fragments arranged by size:
3047 1712 1443 585 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7323 7355 7323
Size: 32 7331
Tth111I GACn'n_nGTC
Cuts at: 1316 4381 1316
Size: 3065 4298
XbaI T'CTAG_A
Cuts at: 4699 4699
Size: 7363
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2377
Size: 7363
XhoI C'TCGA_G
Cuts at: 1943 3389 1943
Size: 1446 5917
XmaI C'CCGG_G
Cuts at: 1870 3760 4688 1870
Size: 1890 928 4545
Fragments arranged by size:
4545 1890 928
XmnI GAAnn'nnTTC
Cuts at: 2012 4535 5492 2012
Size: 2523 957 3883
Fragments arranged by size:
3883 2523 957
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2497 5171 276
Size: 53 83 186 936 963 2674 2468
Fragments arranged by size:
2674 2468 963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I EcoRV FalI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI SfiI
SrfI SwaI