Restriction map for
pFN21AA1633
(Circular) (Six-base) MAPSORT of: pFN21AA1633.seq Check: 4796 from: 1 to: 10147
pFN21AA1633 10147 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2405 2458 2405
Size: 53 10094
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2034 7957 278
Size: 53 83 186 936 498 5923 2468
Fragments arranged by size:
5923 2468 936 498 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4007 4094 7490 1937
Size: 2070 87 3396 4594
Fragments arranged by size:
4594 3396 2070 87
Acc65I G'GTAC_C
Cuts at: 7468 7468
Size: 10147
AclI AA'CG_TT
Cuts at: 1563 5018 8274 8647 1563
Size: 3455 3256 373 3063
Fragments arranged by size:
3455 3256 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2430 3123 3467 4046 4571 4949 5001
Size: 731 67 693 344 579 525 378 52
Cuts at: 5001 5217 5232 6048 6138 6470 6644 6707 7178
Size: 216 15 816 90 332 174 63 471
Cuts at: 7178 8210 9594 9862 1632
Size: 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1032 816 731 693 579 525
471 378 344 332 268 216 174 90
67 63 52 15
AflII C'TTAA_G
Cuts at: 820 1017 3962 4249 820
Size: 197 2945 287 6718
Fragments arranged by size:
6718 2945 287 197
AflIII A'CryG_T
Cuts at: 1203 3474 5548 9046 10023 1203
Size: 2271 2074 3498 977 1327
Fragments arranged by size:
3498 2271 2074 1327 977
AgeI A'CCGG_T
Cuts at: 8978 8978
Size: 10147
AhdI GACnn_n'nnGTC
Cuts at: 8876 8876
Size: 10147
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4318 1184
Size: 3134 7013
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7060 7092 7411 7443 7060
Size: 32 319 32 9764
Fragments arranged by size:
9764 319 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2058 3226 4024 5620 6568 6601 6640 7890 9462
Size: 1168 798 1596 948 33 39 1250 1572
Cuts at: 9462 2058
Size: 2743
Fragments arranged by size:
2743 1596 1572 1250 1168 948 798 39
33
ApaI G_GGCC'C
Cuts at: 4291 5464 4291
Size: 1173 8974
ApaLI G'TGCA_C
Cuts at: 8204 9360 8204
Size: 1156 8991
ApoI r'AATT_y
Cuts at: 1493 2276 3089 3488 3811 3846 5386 5656 6207
Size: 783 813 399 323 35 1540 270 551
Cuts at: 6207 6290 6357 7456 7682 7834 9943 1493
Size: 83 67 1099 226 152 2109 1697
Fragments arranged by size:
2109 1697 1540 1099 813 783 551 399
323 270 226 152 83 67 35
AseI AT'TA_AT
Cuts at: 160 1032 4167 5313 8701 160
Size: 872 3135 1146 3388 1606
Fragments arranged by size:
3388 3135 1606 1146 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10147
AvaI C'yCGr_G
Cuts at: 1870 1943 7184 7472 1870
Size: 73 5241 288 4545
Fragments arranged by size:
5241 4545 288 73
BamHI G'GATC_C
Cuts at: 2877 6410 7477 7844 2877
Size: 3533 1067 367 5180
Fragments arranged by size:
5180 3533 1067 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4747 7468 8923 618
Size: 325 217 627 2960 2721 1455 1842
Fragments arranged by size:
2960 2721 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2322 2781 3558 4291 5228 5464 5763 7411
Size: 931 459 777 733 937 236 299 1648
Cuts at: 7411 7466 1391
Size: 55 4072
Fragments arranged by size:
4072 1648 937 931 777 733 459 299
236 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 3386 3827 4484 6024 6155 6470
Size: 971 1212 275 441 657 1540 131 315
Cuts at: 6470 928
Size: 4605
Fragments arranged by size:
4605 1540 1212 971 657 441 315 275
131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8338 8372 1903
Size: 34 6401 34 3678
Fragments arranged by size:
6401 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 8039 9249 4238
Size: 3801 1210 5136
Fragments arranged by size:
5136 3801 1210
BclI T'GATC_A
Cuts at: 1548 3438 3886 5128 5296 7339 1548
Size: 1890 448 1242 168 2043 4356
Fragments arranged by size:
4356 2043 1890 1242 448 168
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2528 3062 3143
Size: 107 122 71 631 605 856 534 81
Cuts at: 3143 4756 8758 136
Size: 1613 4002 1525
Fragments arranged by size:
4002 1613 1525 856 631 605 534 122
107 81 71
BglII A'GATC_T
Cuts at: 2476 4905 5710 10142 2476
Size: 2429 805 4432 2481
Fragments arranged by size:
4432 2481 2429 805
BlpI GC'TnA_GC
Cuts at: 2051 2404 5031 5922 6602 7310 7564 2051
Size: 353 2627 891 680 708 254 4634
Fragments arranged by size:
4634 2627 891 708 680 353 254
Bme1580I G_kGCm'C
Cuts at: 1790 4291 4596 5464 7202 8208 9364 1790
Size: 2501 305 868 1738 1006 1156 2573
Fragments arranged by size:
2573 2501 1738 1156 1006 868 305
BmgBI CAC'GTC
Cuts at: 1318 3475 1318
Size: 2157 7990
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2070 5410 7878
Size: 416 53 83 399 24 653 3340 2468
Cuts at: 7878 8836 442
Size: 958 1753
Fragments arranged by size:
3340 2468 1753 958 653 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4122 1056
Size: 3066 7081
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5170 6470 6472 7196 5170
Size: 1300 2 724 8121
Fragments arranged by size:
8121 1300 724 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2793 3278 3290 3806 4470 5125 5621
Size: 1100 454 485 12 516 664 655 496
Cuts at: 5621 5642 5951 6442 6497 7189 7409 8807 1239
Size: 21 309 491 55 692 220 1398 2579
Fragments arranged by size:
2579 1398 1100 692 664 655 516 496
491 485 454 309 220 55 21 12
Bpu10I CC'TnA_GC
Cuts at: 3557 4485 6057 7394 3557
Size: 928 1572 1337 6310
Fragments arranged by size:
6310 1572 1337 928
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2276 2306 3572 3936 5494 6162 6388 8272 9137
Size: 30 1266 364 1558 668 226 1884 865
Cuts at: 9137 9435 2276
Size: 298 2988
Fragments arranged by size:
2988 1884 1558 1266 865 668 364 298
226 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6114 8810 882
Size: 617 4615 2696 2219
Fragments arranged by size:
4615 2696 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5542 5551 7938 493
Size: 711 4338 9 2387 2702
Fragments arranged by size:
4338 2702 2387 711 9
BsaBI GATnn'nnATC
Cuts at: 10141 10141
Size: 10147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 7954 8336 8971
Size: 53 83 186 936 498 5923 382 635
Cuts at: 8971 275
Size: 1451
Fragments arranged by size:
5923 1451 936 635 498 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8580 8978 9252 9399 9810 8580
Size: 398 274 147 411 8917
Fragments arranged by size:
8917 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2146 2176 6611 6641 6830
Size: 30 165 30 743 30 4435 30 189
Cuts at: 6830 6836 6860 6866 6868 6898 6990 7020 1178
Size: 6 24 6 2 30 92 30 4305
Fragments arranged by size:
4435 4305 743 189 165 92 30 30
30 30 30 30 24 6 6 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 2744 3845 5864 6632 6810 1186
Size: 1504 54 1101 2019 768 178 4523
Fragments arranged by size:
4523 2019 1504 1101 768 178 54
BseYI C'CCAG_C
Cuts at: 2114 4569 7043 7063 7084 7430 9350 9846 2114
Size: 2455 2474 20 21 346 1920 496 2415
Fragments arranged by size:
2474 2455 2415 1920 496 346 21 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2483 2817 3289 4643 4692 4724 5440
Size: 573 747 334 472 1354 49 32 716
Cuts at: 5440 6632 1163
Size: 1192 4678
Fragments arranged by size:
4678 1354 1192 747 716 573 472 334
49 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7597 8358 9386 1540
Size: 275 186 5596 761 1028 2301
Fragments arranged by size:
5596 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2176 2322 2781 3558 3590 5763 6483 6717 7121
Size: 146 459 777 32 2173 720 234 404
Cuts at: 7121 8208 8293 9364 2176
Size: 1087 85 1071 2959
Fragments arranged by size:
2959 2173 1087 1071 777 720 459 404
234 146 85 32
BsmI GAATG_Cn'
Cuts at: 2271 4319 5032 7658 7751 2271
Size: 2048 713 2626 93 4667
Fragments arranged by size:
4667 2626 2048 713 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2555 4868 2555
Size: 2313 7834
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2322 2781 3558 3590 4291 4596
Size: 399 386 146 459 777 32 701 305
Cuts at: 4596 5228 5464 5763 6483 6717 7121 7202 7411
Size: 632 236 299 720 234 404 81 209
Cuts at: 7411 7466 8208 8293 9364 1391
Size: 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 777 742 720 701 632 459
404 399 386 305 299 236 234 209
146 85 81 55 32
BspEI T'CCGG_A
Cuts at: 9810 9810
Size: 10147
BspHI T'CATG_A
Cuts at: 8036 9766 8036
Size: 1730 8417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2405 2458 3750 4338 4656 4746 7502 844
Size: 1561 53 1292 588 318 90 2756 3489
Fragments arranged by size:
3489 2756 1561 1292 588 318 90 53
BsrBI CCG'CTC
Cuts at: 1394 1823 8034 1394
Size: 429 6211 3507
Fragments arranged by size:
6211 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3352 3378 6402 8642 8816 65
Size: 1183 2104 26 3024 2240 174 1396
Fragments arranged by size:
3024 2240 2104 1396 1183 174 26
BsrFI r'CCGG_y
Cuts at: 1700 1810 8791 8978 1700
Size: 110 6981 187 2869
Fragments arranged by size:
6981 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4504 96
Size: 4408 5739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10147
BssSI C'ACGA_G
Cuts at: 2621 6117 8207 9219 2621
Size: 3496 2090 1012 3549
Fragments arranged by size:
3549 3496 2090 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3796 4747 5533 6640 794
Size: 3002 951 786 1107 4301
Fragments arranged by size:
4301 3002 1107 951 786
BstBI TT'CG_AA
Cuts at: 6211 8951 6211
Size: 2740 7407
BstEII G'GTnAC_C
Cuts at: 1184 7240 1184
Size: 6056 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 4070 4298 5872 4070
Size: 228 1574 8345
Fragments arranged by size:
8345 1574 228
BstYI r'GATC_y
Cuts at: 1972 2476 2877 3547 4905 5682 5710 6284 6410
Size: 504 401 670 1358 777 28 574 126
Cuts at: 6410 7477 7844 8229 8246 9698 9784 9796 10142
Size: 1067 367 385 17 1452 86 12 346
Cuts at: 10142 1972
Size: 1977
Fragments arranged by size:
1977 1452 1358 1067 777 670 574 504
401 385 367 346 126 86 28 17
12
BstZ17I GTA'TAC
Cuts at: 4008 4008
Size: 10147
BtgI C'CryG_G
Cuts at: 513 1065 2976 4635 5794 6747 513
Size: 552 1911 1659 1159 953 3913
Fragments arranged by size:
3913 1911 1659 1159 953 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2651 487
Size: 683 56 1425 7983
Fragments arranged by size:
7983 1425 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 4704 4992 5428 6798 7117 7663 8457 8477
Size: 2636 288 436 1370 319 546 794 20
Cuts at: 8477 2068
Size: 3738
Fragments arranged by size:
3738 2636 1370 794 546 436 319 288
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10112
DraI TTT'AAA
Cuts at: 4939 7451 4939
Size: 2512 7635
DraIII CAC_nnn'GTG
Cuts at: 4747 5533 6888 4747
Size: 786 1355 8006
Fragments arranged by size:
8006 1355 786
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2036 5299 9154 809
Size: 507 720 3263 3855 1802
Fragments arranged by size:
3855 3263 1802 720 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4632 4727 5791
Size: 54 1425 50 275 18 2802 95 1064
Cuts at: 5791 5886 6592 6910 6937 7122 7192 7594 8483
Size: 95 706 318 27 185 70 402 889
Cuts at: 8483 10112 8
Size: 1629 43
Fragments arranged by size:
2802 1629 1425 1064 889 706 402 318
275 185 95 95 70 54 50 43
27 18
EagI C'GGCC_G
Cuts at: 1537 1812 7594 1537
Size: 275 5782 4090
Fragments arranged by size:
5782 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2580 2690 2886 3284 3595 3651 4683
Size: 666 561 110 196 398 311 56 1032
Cuts at: 4683 6705 7134 8077 1353
Size: 2022 429 943 3423
Fragments arranged by size:
3423 2022 1032 943 666 561 429 398
311 196 110 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8734 9108 9254 387
Size: 1064 138 7145 374 146 1280
Fragments arranged by size:
7145 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2320 2779 3556 5761 2320
Size: 459 777 2205 6706
Fragments arranged by size:
6706 2205 777 459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2363 2430 2793 3123 3278 3290
Size: 393 707 24 67 363 330 155 12
Cuts at: 3290 3467 3806 4046 4470 4571 4949 5001 5125
Size: 177 339 240 424 101 378 52 124
Cuts at: 5125 5217 5232 5621 5642 5951 6048 6138 6442
Size: 92 15 389 21 309 97 90 304
Cuts at: 6442 6470 6497 6644 6707 7178 7189 7409 8210
Size: 28 27 147 63 471 11 220 801
Cuts at: 8210 8807 9594 9862 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 801 787 707 597 471 424 393
389 378 363 339 330 309 304 268
240 220 177 155 147 124 101 97
92 90 67 63 52 28 27 24
21 15 12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2726 6959 7139 7370 2726
Size: 4233 180 231 5503
Fragments arranged by size:
5503 4233 231 180
EcoO109I rG'GnC_Cy
Cuts at: 2896 3064 3265 4287 5460 6385 6973 2896
Size: 168 201 1022 1173 925 588 6070
Fragments arranged by size:
6070 1173 1022 925 588 201 168
EcoRI G'AATT_C
Cuts at: 7456 7834 7456
Size: 378 9769
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3977 4517 4698 4831 6127 3977
Size: 540 181 133 1296 7997
Fragments arranged by size:
7997 1296 540 181 133
FspI TGC'GCA
Cuts at: 8653 8653
Size: 10147
HaeII r_GCGC'y
Cuts at: 1678 7321 9294 1678
Size: 5643 1973 2531
Fragments arranged by size:
5643 2531 1973
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2734 2750 2752 2766
Size: 32 135 32 1148 14 16 2 14
Cuts at: 2766 2782 3430 3462 3878 3910 5674 5706 6830
Size: 16 648 32 416 32 1764 32 1124
Cuts at: 6830 6836 6862 6868 6990 7022 8788 8820 8862
Size: 6 26 6 122 32 1766 32 42
Cuts at: 8862 8894 1373
Size: 32 2626
Fragments arranged by size:
2626 1766 1764 1148 1124 648 416 135
122 42 32 32 32 32 32 32
32 32 26 16 16 14 14 6
6 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5819 7491 7737 669
Size: 988 281 3881 1672 246 3079
Fragments arranged by size:
3881 3079 1672 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2597 2706 7160 7298 748
Size: 1849 109 4454 138 3597
Fragments arranged by size:
4454 3597 1849 138 109
HpaI GTT'AAC
Cuts at: 7737 7737
Size: 10147
KpnI G_GTAC'C
Cuts at: 7472 7472
Size: 10147
MfeI C'AATT_G
Cuts at: 7343 7746 7343
Size: 403 9744
MluI A'CGCG_T
Cuts at: 10023 10023
Size: 10147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 5950 6252 9261 9445 1305
Size: 696 3949 302 3009 184 2007
Fragments arranged by size:
3949 3009 2007 696 302 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4634 5793 6912 7194 10
Size: 54 1425 343 2802 1159 1119 282 2963
Fragments arranged by size:
2963 2802 1425 1159 1119 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4461 8105 8464 8623 518
Size: 648 3295 3644 359 159 2042
Fragments arranged by size:
3644 3295 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2371 2434 4894 5569 5620 5908 6490
Size: 449 316 63 2460 675 51 288 582
Cuts at: 6490 6568 6585 6591 6640 7309 7563 8240 9388
Size: 78 17 6 49 669 254 677 1148
Cuts at: 9388 9633 1606
Size: 245 2120
Fragments arranged by size:
2460 2120 1148 677 675 669 582 449
316 288 254 245 78 63 51 49
17 6
NcoI C'CATG_G
Cuts at: 513 1065 2976 5794 6747 513
Size: 552 1911 2818 953 3913
Fragments arranged by size:
3913 2818 1911 953 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10147
NheI G'CTAG_C
Cuts at: 1052 4118 1052
Size: 3066 7081
NotI GC'GGCC_GC
Cuts at: 7594 7594
Size: 10147
NspI r_CATG'y
Cuts at: 1132 1229 2751 5668 7425 7505 9050 1132
Size: 97 1522 2917 1757 80 1545 2229
Fragments arranged by size:
2917 2229 1757 1545 1522 97 80
PasI CC'CwG_GG
Cuts at: 1348 2042 1348
Size: 694 9453
PciI A'CATG_T
Cuts at: 9046 9046
Size: 10147
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4882 4909 5359 6830 6949 1108
Size: 3774 27 450 1471 119 4306
Fragments arranged by size:
4306 3774 1471 450 119 27
PfoI T'CCnGG_A
Cuts at: 2355 2649 5898 6458 6573 7414 2355
Size: 294 3249 560 115 841 5088
Fragments arranged by size:
5088 3249 841 560 294 115
PmeI GTTT'AAAC
Cuts at: 7451 7451
Size: 10147
PmlI CAC'GTG
Cuts at: 5551 5551
Size: 10147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8216 8248 9729 9761 8216
Size: 32 1481 32 8602
Fragments arranged by size:
8602 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2896 3265 2896
Size: 369 9778
PshAI GACnn'nnGTC
Cuts at: 1613 4090 1613
Size: 2477 7670
PsiI TTA'TAA
Cuts at: 7717 7717
Size: 10147
PspOMI G'GGCC_C
Cuts at: 4287 5460 4287
Size: 1173 8974
PstI C_TGCA'G
Cuts at: 830 2063 3544 3720 3747 4899 4920 5646 5697
Size: 1233 1481 176 27 1152 21 726 51
Cuts at: 5697 5862 6531 6590 7499 830
Size: 165 669 59 909 3478
Fragments arranged by size:
3478 1481 1233 1152 909 726 669 176
165 59 51 27 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10147
PvuII CAG'CTG
Cuts at: 2371 4894 5620 5908 6568 6640 7309 2371
Size: 2523 726 288 660 72 669 5209
Fragments arranged by size:
5209 2523 726 669 660 288 72
RsrII CG'GwC_CG
Cuts at: 5564 5564
Size: 10147
SacI G_AGCT'C
Cuts at: 2322 2781 3558 5763 2322
Size: 459 777 2205 6706
Fragments arranged by size:
6706 2205 777 459
SalI G'TCGA_C
Cuts at: 1936 7489 1936
Size: 5553 4594
SapI GCTCTTCn'nnn_
Cuts at: 2580 2690 3284 3595 3651 6705 2580
Size: 110 594 311 56 3054 6022
Fragments arranged by size:
6022 3054 594 311 110 56
SbfI CC_TGCA'GG
Cuts at: 3747 7499 3747
Size: 3752 6395
ScaI AGT'ACT
Cuts at: 8395 10107 8395
Size: 1712 8435
SfcI C'TryA_G
Cuts at: 826 1045 2059 2925 3068 3540 3716 3743 4095
Size: 219 1014 866 143 472 176 27 352
Cuts at: 4095 4895 4916 5642 5693 5858 6527 6586 7147
Size: 800 21 726 51 165 669 59 561
Cuts at: 7147 7495 8630 9311 9502 826
Size: 348 1135 681 191 1471
Fragments arranged by size:
1471 1135 1014 866 800 726 681 669
561 472 352 348 219 191 176 165
143 59 51 27 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10147
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10147
SmaI CCC'GGG
Cuts at: 1872 7474 1872
Size: 5602 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 2321 3551 3951 3962 4249
Size: 197 926 312 66 1230 400 11 287
Cuts at: 4249 5473 6177 6367 8251 9152 9414 820
Size: 1224 704 190 1884 901 262 1553
Fragments arranged by size:
1884 1553 1230 1224 926 901 704 400
312 287 262 197 190 66 11
SnaBI TAC'GTA
Cuts at: 493 7938 493
Size: 7445 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10147
SphI G_CATG'C
Cuts at: 1132 7505 1132
Size: 6373 3774
SspI AAT'ATT
Cuts at: 5 52 8071 5
Size: 47 8019 2081
Fragments arranged by size:
8019 2081 47
StuI AGG'CCT
Cuts at: 1517 2546 7145 7399 1517
Size: 1029 4599 254 4265
Fragments arranged by size:
4599 4265 1029 254
StyI C'CwwG_G
Cuts at: 513 1065 2801 2976 3825 4596 5794 6747 7586
Size: 552 1736 175 849 771 1198 953 839
Cuts at: 7586 513
Size: 3074
Fragments arranged by size:
3074 1736 1198 953 849 839 771 552
175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3233 4743 4889 5552 6002 7098 8174
Size: 1255 1653 1510 146 663 450 1096 1076
Cuts at: 8174 8191 8344 8529 8974 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1653 1510 1498 1255 1096 1076 663 450
445 185 153 146 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3176 4504 8393
Size: 274 80 33 51 1443 1199 1328 3889
Cuts at: 8393 10105 96
Size: 1712 138
Fragments arranged by size:
3889 1712 1443 1328 1199 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7181 7213 10107 10139 7181
Size: 32 2894 32 7189
Fragments arranged by size:
7189 2894 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10147
XbaI T'CTAG_A
Cuts at: 7483 7483
Size: 10147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2116 4366 4879 7132 7384 2011
Size: 105 2250 513 2253 252 4774
Fragments arranged by size:
4774 2253 2250 513 252 105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10147
XmaI C'CCGG_G
Cuts at: 1870 7472 1870
Size: 5602 4545
XmnI GAAnn'nnTTC
Cuts at: 4410 5217 5702 6294 8276 4410
Size: 807 485 592 1982 6281
Fragments arranged by size:
6281 1982 807 592 485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2032 7955 276
Size: 53 83 186 936 498 5923 2468
Fragments arranged by size:
5923 2468 936 498 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI SacII SanDI SexAI
SfiI SfoI SrfI SwaI