Restriction map for
pFN21AA1628
(Circular) (Six-base) MAPSORT of: pFN21AA1628.seq Check: 3142 from: 1 to: 8455
pFN21AA1628 8455 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2271 3667 2271
Size: 1396 7059
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6265 278
Size: 53 83 186 936 4729 2468
Fragments arranged by size:
4729 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5798 1937
Size: 3861 4594
Acc65I G'GTAC_C
Cuts at: 5776 5776
Size: 8455
AclI AA'CG_TT
Cuts at: 1563 6582 6955 1563
Size: 5019 373 3063
Fragments arranged by size:
5019 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 2653 3761 4348 4705 5145 5178 5418
Size: 947 74 1108 587 357 440 33 240
Cuts at: 5418 6518 7902 8170 1632
Size: 1100 1384 268 1917
Fragments arranged by size:
1917 1384 1108 1100 947 587 440 357
268 240 74 33
AfeI AGC'GCT
Cuts at: 3099 4523 3099
Size: 1424 7031
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8258
AflIII A'CryG_T
Cuts at: 1203 7354 8331 1203
Size: 6151 977 1327
Fragments arranged by size:
6151 1327 977
AgeI A'CCGG_T
Cuts at: 7286 7286
Size: 8455
AhdI GACnn_n'nnGTC
Cuts at: 7184 7184
Size: 8455
AleI CACnn'nnGTG
Cuts at: 1166 2210 2765 2768 4787 1166
Size: 1044 555 3 2019 4834
Fragments arranged by size:
4834 2019 1044 555 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8455
AlwNI CAG_nnn'CTG
Cuts at: 2330 2602 2984 4371 5311 5326 6198 7770 2330
Size: 272 382 1387 940 15 872 1572 3015
Fragments arranged by size:
3015 1572 1387 940 872 382 272 15
ApaI G_GGCC'C
Cuts at: 2150 3838 5281 2150
Size: 1688 1443 5324
Fragments arranged by size:
5324 1688 1443
ApaLI G'TGCA_C
Cuts at: 3563 5069 5129 6512 7668 3563
Size: 1506 60 1383 1156 4350
Fragments arranged by size:
4350 1506 1383 1156 60
ApoI r'AATT_y
Cuts at: 1493 5764 5990 6142 8251 1493
Size: 4271 226 152 2109 1697
Fragments arranged by size:
4271 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7009 160
Size: 872 5977 1606
Fragments arranged by size:
5977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8455
AvaI C'yCGr_G
Cuts at: 1870 1943 3026 3359 4991 5274 5506 5579 5780
Size: 73 1083 333 1632 283 232 73 201
Cuts at: 5780 1870
Size: 4545
Fragments arranged by size:
4545 1632 1083 333 283 232 201 73
73
BamHI G'GATC_C
Cuts at: 5518 5785 6152 5518
Size: 267 367 7821
Fragments arranged by size:
7821 367 267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3022 3121 4216 4222 4608
Size: 325 217 627 1235 99 1095 6 386
Cuts at: 4608 5776 7231 618
Size: 1168 1455 1842
Fragments arranged by size:
1842 1455 1235 1168 1095 627 386 325
217 99 6
BanII G_rGCy'C
Cuts at: 1391 2040 2150 2991 3838 4380 4577 4626 5020
Size: 649 110 841 847 542 197 49 394
Cuts at: 5020 5197 5281 5774 1391
Size: 177 84 493 4072
Fragments arranged by size:
4072 847 841 649 542 493 394 197
177 110 84 49
BbeI G_GCGC'C
Cuts at: 3026 3125 4220 3026
Size: 99 1095 7261
Fragments arranged by size:
7261 1095 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4428 928
Size: 971 2529 4955
Fragments arranged by size:
4955 2529 971
BbvCI CC'TCA_GC
Cuts at: 2806 4014 4728 2806
Size: 1208 714 6533
Fragments arranged by size:
6533 1208 714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6646 6680 1903
Size: 34 4709 34 3678
Fragments arranged by size:
4709 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6347 7557 6347
Size: 1210 7245
BclI T'GATC_A
Cuts at: 1548 4824 1548
Size: 3276 5179
BfrBI ATG'CAT
Cuts at: 3853 3853
Size: 8455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3130 3139 4084
Size: 107 122 71 631 605 1458 9 945
Cuts at: 4084 4540 7066 136
Size: 456 2526 1525
Fragments arranged by size:
2526 1525 1458 945 631 605 456 122
107 71 9
BglII A'GATC_T
Cuts at: 3975 5184 8450 3975
Size: 1209 3266 3980
Fragments arranged by size:
3980 3266 1209
BlpI GC'TnA_GC
Cuts at: 2111 2985 5210 5872 2111
Size: 874 2225 662 4694
Fragments arranged by size:
4694 2225 874 662
Bme1580I G_kGCm'C
Cuts at: 1790 2150 3567 3838 4613 5073 5133 5281 6516
Size: 360 1417 271 775 460 60 148 1235
Cuts at: 6516 7672 1790
Size: 1156 2573
Fragments arranged by size:
2573 1417 1235 1156 775 460 360 271
148 60
BmgBI CAC'GTC
Cuts at: 1318 2734 4270 5110 1318
Size: 1416 1536 840 4663
Fragments arranged by size:
4663 1536 1416 840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 3838 3861
Size: 416 53 83 399 24 2162 259 23
Cuts at: 3861 5310 6186 7144 442
Size: 1449 876 958 1753
Fragments arranged by size:
2162 1753 1449 958 876 416 399 259
83 53 24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8455
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4770 4770
Size: 8455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2239 2264 4245 4437 4763 4817 4996 5469
Size: 1000 25 1981 192 326 54 179 473
Cuts at: 5469 5483 5627 5694 7115 1239
Size: 14 144 67 1421 2579
Fragments arranged by size:
2579 1981 1421 1000 473 326 192 179
144 67 54 25 14
Bpu10I CC'TnA_GC
Cuts at: 2806 3665 4014 4379 4680 4728 2806
Size: 859 349 365 301 48 6533
Fragments arranged by size:
6533 859 365 349 301 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4073 6580 7445 7743 4073
Size: 2507 865 298 4785
Fragments arranged by size:
4785 2507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4050 5660 5746 7118 882
Size: 617 2551 1610 86 1372 2219
Fragments arranged by size:
2551 2219 1610 1372 617 86
BsaAI yAC'GTr
Cuts at: 493 1204 3511 6246 493
Size: 711 2307 2735 2702
Fragments arranged by size:
2735 2702 2307 711
BsaBI GATnn'nnATC
Cuts at: 8449 8449
Size: 8455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 2589 2714 2928
Size: 53 83 186 936 486 570 125 214
Cuts at: 2928 3023 3122 3201 3863 4217 5337 6262 6644
Size: 95 99 79 662 354 1120 925 382
Cuts at: 6644 7279 275
Size: 635 1451
Fragments arranged by size:
1451 1120 936 925 662 635 570 486
382 354 214 186 125 99 95 83
79 53
BsaWI w'CCGG_w
Cuts at: 6888 7286 7560 7707 8118 6888
Size: 398 274 147 411 7225
Fragments arranged by size:
7225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 4529 4559 4829
Size: 30 165 30 666 30 2430 30 270
Cuts at: 4829 4859 1178
Size: 30 4774
Fragments arranged by size:
4774 2430 666 270 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2483 2573 4031 4034 4895 5678 5734
Size: 844 453 90 1458 3 861 783 56
Cuts at: 5734 5737 1186
Size: 3 3904
Fragments arranged by size:
3904 1458 861 844 783 453 90 56
3 3
BseYI C'CCAG_C
Cuts at: 2292 3290 3525 3529 3553 3597 3659 4119 5033
Size: 998 235 4 24 44 62 460 914
Cuts at: 5033 5041 5214 5371 7658 8154 2292
Size: 8 173 157 2287 496 2593
Fragments arranged by size:
2593 2287 998 914 496 460 235 173
157 62 44 24 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 3095 3609 3938 4493 4586 1163
Size: 573 693 666 514 329 555 93 5032
Fragments arranged by size:
5032 693 666 573 555 514 329 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 2032 4036 5264 5905 6666
Size: 275 186 25 6 2004 1228 641 761
Cuts at: 6666 7694 1540
Size: 1028 2301
Fragments arranged by size:
2301 2004 1228 1028 761 641 275 186
25 6
BsiHKAI G_wGCw'C
Cuts at: 2250 2991 3056 3567 4686 5020 5073 5133 6516
Size: 741 65 511 1119 334 53 60 1383
Cuts at: 6516 6601 7672 2250
Size: 85 1071 3033
Fragments arranged by size:
3033 1383 1119 1071 741 511 334 85
65 60 53
BsmI GAATG_Cn'
Cuts at: 4390 5966 6059 4390
Size: 1576 93 6786
Fragments arranged by size:
6786 1576 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2090 2090
Size: 8455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2150 2250 2991 3056 3567 3838
Size: 399 250 110 100 741 65 511 271
Cuts at: 3838 4380 4577 4613 4626 4686 5020 5073 5133
Size: 542 197 36 13 60 334 53 60
Cuts at: 5133 5197 5281 5774 6516 6601 7672 1391
Size: 64 84 493 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 542 511 493 399
334 271 250 197 110 100 85 84
65 64 60 60 53 36 13
BspEI T'CCGG_A
Cuts at: 8118 8118
Size: 8455
BspHI T'CATG_A
Cuts at: 6344 8074 6344
Size: 1730 6725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 3667 4572 5606 5810 844
Size: 1427 1396 905 1034 204 3489
Fragments arranged by size:
3489 1427 1396 1034 905 204
BsrBI CCG'CTC
Cuts at: 1394 1823 3355 6342 1394
Size: 429 1532 2987 3507
Fragments arranged by size:
3507 2987 1532 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2549 3676 5356 6950 7124 65
Size: 1183 1301 1127 1680 1594 174 1396
Fragments arranged by size:
1680 1594 1396 1301 1183 1127 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 3203 3824 4134 4219 4539 5263
Size: 110 211 1182 621 310 85 320 724
Cuts at: 5263 7099 7286 1700
Size: 1836 187 2869
Fragments arranged by size:
2869 1836 1182 724 621 320 310 211
187 110 85
BsrGI T'GTAC_A
Cuts at: 96 4656 96
Size: 4560 3895
BssHII G'CGCG_C
Cuts at: 1925 2069 3059 4778 1925
Size: 144 990 1719 5602
Fragments arranged by size:
5602 1719 990 144
BssSI C'ACGA_G
Cuts at: 4154 6515 7527 4154
Size: 2361 1012 5082
Fragments arranged by size:
5082 2361 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5618 794
Size: 4824 3631
BstBI TT'CG_AA
Cuts at: 7259 7259
Size: 8455
BstEII G'GTnAC_C
Cuts at: 1184 2212 4396 1184
Size: 1028 2184 5243
Fragments arranged by size:
5243 2184 1028
BstXI CCAn_nnnn'nTGG
Cuts at: 3980 3980
Size: 8455
BstYI r'GATC_y
Cuts at: 1972 2598 3975 5184 5518 5732 5785 6152 6537
Size: 626 1377 1209 334 214 53 367 385
Cuts at: 6537 6554 8006 8092 8104 8450 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1377 1209 626 385 367 346
334 214 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2331 2556 5469 2331
Size: 225 2913 5317
Fragments arranged by size:
5317 2913 225
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 2032 2374 3089 3267 4051
Size: 552 939 22 6 342 715 178 784
Cuts at: 4051 4590 4735 513
Size: 539 145 4233
Fragments arranged by size:
4233 939 784 715 552 539 342 178
145 22 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7716
Fragments arranged by size:
7716 683 56
BtsI GCAGTG_nn'
Cuts at: 2129 2169 2320 2973 3309 3450 4197 4476 5971
Size: 40 151 653 336 141 747 279 1495
Cuts at: 5971 6765 6785 2129
Size: 794 20 3799
Fragments arranged by size:
3799 1495 794 747 653 336 279 151
141 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2806 2841 529
Size: 35 2242 35 6143
Fragments arranged by size:
6143 2242 35 35
DraI TTT'AAA
Cuts at: 5759 5759
Size: 8455
DraIII CAC_nnn'GTG
Cuts at: 4486 4486
Size: 8455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 7462 809
Size: 507 1008 5138 1802
Fragments arranged by size:
5138 1802 1008 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2029 2064
Size: 54 1425 50 275 18 193 6 35
Cuts at: 2064 2394 2643 2776 3092 3264 4132 5261 5902
Size: 330 249 133 316 172 868 1129 641
Cuts at: 5902 6791 8420 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1129 889 868 641 330 316
275 249 193 172 133 54 50 43
35 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2023 2029 5261 5902 1537
Size: 275 211 6 3232 641 4090
Fragments arranged by size:
4090 3232 641 275 211 6
EarI CTCTTCn'nnn_
Cuts at: 1353 6385 1353
Size: 5032 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7042 7416 7562 387
Size: 1064 138 5453 374 146 1280
Fragments arranged by size:
5453 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2989 5018 2989
Size: 2029 6426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2239 2264 2579 2653 3761 4245 4348
Size: 393 607 25 315 74 1108 484 103
Cuts at: 4348 4437 4705 4763 4817 4996 5145 5178 5418
Size: 89 268 58 54 179 149 33 240
Cuts at: 5418 5469 5483 5627 5694 6518 7115 7902 8170
Size: 51 14 144 67 824 597 787 268
Cuts at: 8170 1239
Size: 1524
Fragments arranged by size:
1524 1108 824 787 607 597 484 393
315 268 268 240 179 149 144 103
89 74 67 58 54 51 33 25
14
EcoO109I rG'GnC_Cy
Cuts at: 2627 2680 2706 3478 3834 3835 4076 4793 5255
Size: 53 26 772 356 1 241 717 462
Cuts at: 5255 5278 5473 2627
Size: 23 195 5609
Fragments arranged by size:
5609 772 717 462 356 241 195 53
26 23 1
EcoRI G'AATT_C
Cuts at: 5764 6142 5764
Size: 378 8077
FspI TGC'GCA
Cuts at: 6961 6961
Size: 8455
HaeII r_GCGC'y
Cuts at: 1678 3026 3035 3101 3125 3297 4220 4525 7602
Size: 1348 9 66 24 172 923 305 3077
Cuts at: 7602 1678
Size: 2531
Fragments arranged by size:
3077 2531 1348 923 305 172 66 24
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3020 3052 4410 4442 4529
Size: 32 135 32 1448 32 1358 32 87
Cuts at: 4529 4561 4829 4850 4861 4882 5489 5521 5724
Size: 32 268 21 11 21 607 32 203
Cuts at: 5724 5756 7096 7128 7170 7202 1373
Size: 32 1340 32 42 32 2626
Fragments arranged by size:
2626 1448 1358 1340 607 268 203 135
87 42 32 32 32 32 32 32
32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2049 4348 5799 6045 669
Size: 988 281 111 2299 1451 246 3079
Fragments arranged by size:
3079 2299 1451 988 281 246 111
HindIII A'AGCT_T
Cuts at: 748 2417 748
Size: 1669 6786
HpaI GTT'AAC
Cuts at: 6045 6045
Size: 8455
KasI G'GCGC_C
Cuts at: 3022 3121 4216 3022
Size: 99 1095 7261
Fragments arranged by size:
7261 1095 99
KpnI G_GTAC'C
Cuts at: 5780 5780
Size: 8455
MfeI C'AATT_G
Cuts at: 6054 6054
Size: 8455
MluI A'CGCG_T
Cuts at: 8331 8331
Size: 8455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2863 4584 7569 7753 1305
Size: 1558 1721 2985 184 2007
Fragments arranged by size:
2985 2007 1721 1558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 2645 2778 10
Size: 54 1425 343 564 249 133 5687
Fragments arranged by size:
5687 1425 564 343 249 133 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2765 2768 3635 4176 4272 4787
Size: 648 1044 555 3 867 541 96 515
Cuts at: 4787 4883 6413 6772 6931 518
Size: 96 1530 359 159 2042
Fragments arranged by size:
2042 1530 1044 867 648 555 541 515
359 159 96 96 3
MspA1I CmG'CkG
Cuts at: 1606 2028 2195 2637 2810 2984 3091 3127 3335
Size: 422 167 442 173 174 107 36 208
Cuts at: 3335 3529 5045 5871 6548 7696 7941 1606
Size: 194 1516 826 677 1148 245 2120
Fragments arranged by size:
2120 1516 1148 826 677 442 422 245
208 194 174 173 167 107 36
NaeI GCC'GGC
Cuts at: 2023 3205 3826 4136 4221 5265 2023
Size: 1182 621 310 85 1044 5213
Fragments arranged by size:
5213 1182 1044 621 310 85
NarI GG'CG_CC
Cuts at: 3023 3122 4217 3023
Size: 99 1095 7261
Fragments arranged by size:
7261 1095 99
NcoI C'CATG_G
Cuts at: 513 1065 2004 4735 513
Size: 552 939 2731 4233
Fragments arranged by size:
4233 2731 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8455
NgoMIV G'CCGG_C
Cuts at: 2021 3203 3824 4134 4219 5263 2021
Size: 1182 621 310 85 1044 5213
Fragments arranged by size:
5213 1182 1044 621 310 85
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8455
NotI GC'GGCC_GC
Cuts at: 5902 5902
Size: 8455
NsiI A_TGCA'T
Cuts at: 3855 3855
Size: 8455
NspI r_CATG'y
Cuts at: 1132 1229 4234 4864 4927 5813 7358 1132
Size: 97 3005 630 63 886 1545 2229
Fragments arranged by size:
3005 2229 1545 886 630 97 63
PasI CC'CwG_GG
Cuts at: 1348 4371 1348
Size: 3023 5432
PciI A'CATG_T
Cuts at: 7354 7354
Size: 8455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2785 3220 4741 5056 5461 1108
Size: 1677 435 1521 315 405 4102
Fragments arranged by size:
4102 1677 1521 435 405 315
PfoI T'CCnGG_A
Cuts at: 2601 4833 2601
Size: 2232 6223
PmeI GTTT'AAAC
Cuts at: 5759 5759
Size: 8455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6524 6556 8037 8069 6524
Size: 32 1481 32 6910
Fragments arranged by size:
6910 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2706 3478 4793 5473 2706
Size: 772 1315 680 5688
Fragments arranged by size:
5688 1315 772 680
PshAI GACnn'nnGTC
Cuts at: 1613 2408 1613
Size: 795 7660
PsiI TTA'TAA
Cuts at: 6025 6025
Size: 8455
PspOMI G'GGCC_C
Cuts at: 2146 3834 5277 2146
Size: 1688 1443 5324
Fragments arranged by size:
5324 1688 1443
PstI C_TGCA'G
Cuts at: 830 2164 2980 3198 3907 4818 5322 5381 5807
Size: 1334 816 218 709 911 504 59 426
Cuts at: 5807 830
Size: 3478
Fragments arranged by size:
3478 1334 911 816 709 504 426 218
59
PvuI CG_AT'CG
Cuts at: 2001 4036 2001
Size: 2035 6420
PvuII CAG'CTG
Cuts at: 2810 3529 5045 2810
Size: 719 1516 6220
Fragments arranged by size:
6220 1516 719
SacI G_AGCT'C
Cuts at: 2991 5020 2991
Size: 2029 6426
SacII CC_GC'GG
Cuts at: 2029 3092 2029
Size: 1063 7392
SalI G'TCGA_C
Cuts at: 1936 5797 1936
Size: 3861 4594
SanDI GG'GwC_CC
Cuts at: 2706 2706
Size: 8455
SbfI CC_TGCA'GG
Cuts at: 3198 4818 5381 5807 3198
Size: 1620 563 426 5846
Fragments arranged by size:
5846 1620 563 426
ScaI AGT'ACT
Cuts at: 6703 8415 6703
Size: 1712 6743
SexAI A'CCwGG_T
Cuts at: 2541 5385 2541
Size: 2844 5611
SfcI C'TryA_G
Cuts at: 826 1045 2160 2173 2976 3194 3467 3903 4814
Size: 219 1115 13 803 218 273 436 911
Cuts at: 4814 5318 5377 5803 6938 7619 7810 826
Size: 504 59 426 1135 681 191 1471
Fragments arranged by size:
1471 1135 1115 911 803 681 504 436
426 273 219 218 191 59 13
SfoI GGC'GCC
Cuts at: 3024 3123 4218 3024
Size: 99 1095 7261
Fragments arranged by size:
7261 1095 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8455
SmaI CCC'GGG
Cuts at: 1872 4993 5276 5581 5782 1872
Size: 3121 283 305 201 4545
Fragments arranged by size:
4545 3121 305 283 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4088 6559 7460 7722 820
Size: 197 926 2145 2471 901 262 1553
Fragments arranged by size:
2471 2145 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6246 493
Size: 5753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8455
SphI G_CATG'C
Cuts at: 1132 5813 1132
Size: 4681 3774
SspI AAT'ATT
Cuts at: 5 52 6379 5
Size: 47 6327 2081
Fragments arranged by size:
6327 2081 47
StuI AGG'CCT
Cuts at: 1517 4971 5724 1517
Size: 3454 753 4248
Fragments arranged by size:
4248 3454 753
StyI C'CwwG_G
Cuts at: 513 1065 2004 4735 5894 513
Size: 552 939 2731 1159 3074
Fragments arranged by size:
3074 2731 1159 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2320 3986 4877 5132 6482 6499 6652
Size: 1255 740 1666 891 255 1350 17 153
Cuts at: 6652 6837 7282 325
Size: 185 445 1498
Fragments arranged by size:
1666 1498 1350 1255 891 740 445 255
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3687 4656 5007
Size: 274 80 33 51 1443 1710 969 351
Cuts at: 5007 6701 8413 96
Size: 1694 1712 138
Fragments arranged by size:
1712 1710 1694 1443 969 351 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2690 2722 4943 4975 8415 8447 2690
Size: 32 2221 32 3440 32 2698
Fragments arranged by size:
3440 2698 2221 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2735 4585 1316
Size: 1419 1850 5186
Fragments arranged by size:
5186 1850 1419
XbaI T'CTAG_A
Cuts at: 5791 5791
Size: 8455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3102 3161 3556 4127 3102
Size: 59 395 571 7430
Fragments arranged by size:
7430 571 395 59
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8455
XmaI C'CCGG_G
Cuts at: 1870 4991 5274 5579 5780 1870
Size: 3121 283 305 201 4545
Fragments arranged by size:
4545 3121 305 283 201
XmnI GAAnn'nnTTC
Cuts at: 4448 6584 4448
Size: 2136 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6263 276
Size: 53 83 186 936 4729 2468
Fragments arranged by size:
4729 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstZ17I EcoNI EcoRV
FalI FseI FspAI NruI PacI PmlI PspXI PsrI
RsrII SapI SfiI SrfI SwaI