Restriction map for
pFN21AA1622
(Circular) (Six-base) MAPSORT of: pFN21AA1622.seq Check: 8384 from: 1 to: 7324
pFN21AA1622 7324 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5134 278
Size: 53 83 186 936 3598 2468
Fragments arranged by size:
3598 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 4667 1937
Size: 1461 1269 4594
Fragments arranged by size:
4594 1461 1269
Acc65I G'GTAC_C
Cuts at: 4645 4645
Size: 7324
AclI AA'CG_TT
Cuts at: 1563 5451 5824 1563
Size: 3888 373 3063
Fragments arranged by size:
3888 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3249 3509 3923 3932 5387 6771 7039 1632
Size: 1617 260 414 9 1455 1384 268 1917
Fragments arranged by size:
1917 1617 1455 1384 414 268 260 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7127
AflIII A'CryG_T
Cuts at: 1203 3547 6223 7200 1203
Size: 2344 2676 977 1327
Fragments arranged by size:
2676 2344 1327 977
AgeI A'CCGG_T
Cuts at: 6155 6155
Size: 7324
AhdI GACnn_n'nnGTC
Cuts at: 3698 6053 3698
Size: 2355 4969
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3680 1184
Size: 2496 4828
AlwNI CAG_nnn'CTG
Cuts at: 3473 5067 6639 3473
Size: 1594 1572 4158
Fragments arranged by size:
4158 1594 1572
ApaLI G'TGCA_C
Cuts at: 5381 6537 5381
Size: 1156 6168
ApoI r'AATT_y
Cuts at: 1493 2589 2612 2982 3017 3286 3776 4633 4859
Size: 1096 23 370 35 269 490 857 226
Cuts at: 4859 5011 7120 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1096 857 490 370 269 226
152 35 23
AseI AT'TA_AT
Cuts at: 160 1032 2771 3713 5878 160
Size: 872 1739 942 2165 1606
Fragments arranged by size:
2165 1739 1606 942 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7324
AvaI C'yCGr_G
Cuts at: 1870 1943 4649 1870
Size: 73 2706 4545
Fragments arranged by size:
4545 2706 73
BamHI G'GATC_C
Cuts at: 4654 5021 4654
Size: 367 6957
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4645 6100 618
Size: 325 217 627 2858 1455 1842
Fragments arranged by size:
2858 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2085 4643 1391
Size: 694 2558 4072
Fragments arranged by size:
4072 2558 694
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 928
Size: 971 264 6089
Fragments arranged by size:
6089 971 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3636 3670 5515 5549 1903
Size: 34 50 34 1615 34 1845 34 3678
Fragments arranged by size:
3678 1845 1615 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3760 5216 6426 3760
Size: 1456 1210 4658
Fragments arranged by size:
4658 1456 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7324
BfrBI ATG'CAT
Cuts at: 2008 2390 2459 3657 2008
Size: 382 69 1198 5675
Fragments arranged by size:
5675 1198 382 69
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5935 136
Size: 107 122 71 631 605 4263 1525
Fragments arranged by size:
4263 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7319 7319
Size: 7324
BlpI GC'TnA_GC
Cuts at: 4741 4741
Size: 7324
Bme1580I G_kGCm'C
Cuts at: 1790 5385 6541 1790
Size: 3595 1156 2573
Fragments arranged by size:
3595 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5055 6013 442
Size: 416 53 83 399 24 3638 958 1753
Fragments arranged by size:
3638 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2976 3312 3365 5984 1239
Size: 1211 526 336 53 2619 2579
Fragments arranged by size:
2619 2579 1211 526 336 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 3025 5449 6314 6612 2102
Size: 923 2424 865 298 2814
Fragments arranged by size:
2814 2424 923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5987 882
Size: 617 4488 2219
Fragments arranged by size:
4488 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5115 493
Size: 711 3911 2702
Fragments arranged by size:
3911 2702 711
BsaBI GATnn'nnATC
Cuts at: 3930 7318 3930
Size: 3388 3936
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5131 5513 6148 275
Size: 53 83 186 936 3598 382 635 1451
Fragments arranged by size:
3598 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2125 4464 5757 6155 6429 6576 6987 2125
Size: 2339 1293 398 274 147 411 2462
Fragments arranged by size:
2462 2339 1293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7099
Fragments arranged by size:
7099 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7324
BseYI C'CCAG_C
Cuts at: 6527 7023 6527
Size: 496 6828
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6751
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4774 5535 6563 1540
Size: 275 186 2773 761 1028 2301
Fragments arranged by size:
2773 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2436 2763 5385 5470 6541 2085
Size: 351 327 2622 85 1071 2868
Fragments arranged by size:
2868 2622 1071 351 327 85
BsmI GAATG_Cn'
Cuts at: 4835 4928 4835
Size: 93 7231
BsmBI CGTCTCn'nnnn_
Cuts at: 2261 2261
Size: 7324
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2436 2763 4643 5385 5470 6541
Size: 399 295 351 327 1880 742 85 1071
Cuts at: 6541 1391
Size: 2174
Fragments arranged by size:
2174 1880 1071 742 399 351 327 295
85
BspEI T'CCGG_A
Cuts at: 4464 6987 4464
Size: 2523 4801
BspHI T'CATG_A
Cuts at: 3511 3603 5213 6943 3511
Size: 92 1610 1730 3892
Fragments arranged by size:
3892 1730 1610 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 3838 4679 844
Size: 1238 1756 841 3489
Fragments arranged by size:
3489 1756 1238 841
BsrBI CCG'CTC
Cuts at: 1394 1823 5211 1394
Size: 429 3388 3507
Fragments arranged by size:
3507 3388 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 5819 5993 65
Size: 1183 1213 3358 174 1396
Fragments arranged by size:
3358 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 5968 6155 1700
Size: 110 291 3867 187 2869
Fragments arranged by size:
3867 2869 291 187 110
BsrGI T'GTAC_A
Cuts at: 96 3035 96
Size: 2939 4385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7324
BssSI C'ACGA_G
Cuts at: 5384 6396 5384
Size: 1012 6312
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4231 794
Size: 3437 3887
BstBI TT'CG_AA
Cuts at: 4318 6128 4318
Size: 1810 5514
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7324
BstXI CCAn_nnnn'nTGG
Cuts at: 2732 2732
Size: 7324
BstYI r'GATC_y
Cuts at: 1972 4654 5021 5406 5423 6875 6961 6973 7319
Size: 2682 367 385 17 1452 86 12 346
Cuts at: 7319 1972
Size: 1977
Fragments arranged by size:
2682 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 4531 4586 513
Size: 552 3466 55 3251
Fragments arranged by size:
3466 3251 552 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2045 2357 487
Size: 683 56 819 312 5454
Fragments arranged by size:
5454 819 683 312 56
BtsI GCAGTG_nn'
Cuts at: 2806 3421 4570 4840 5634 5654 2806
Size: 615 1149 270 794 20 4476
Fragments arranged by size:
4476 1149 794 615 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2736 2771 4546 4581 529
Size: 35 2172 35 1775 35 3272
Fragments arranged by size:
3272 2172 1775 35 35 35
DraI TTT'AAA
Cuts at: 3498 4628 3498
Size: 1130 6194
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2447 6331 809
Size: 507 1131 3884 1802
Fragments arranged by size:
3884 1802 1131 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3150 4771 5660
Size: 54 1425 50 275 18 1320 1621 889
Cuts at: 5660 7289 8
Size: 1629 43
Fragments arranged by size:
1629 1621 1425 1320 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4771 1537
Size: 275 2959 4090
Fragments arranged by size:
4090 2959 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 4273 5254 1353
Size: 1125 1795 981 3423
Fragments arranged by size:
3423 1795 1125 981
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2001 2096 5911 6285 6431 387
Size: 1064 138 412 95 3815 374 146 1280
Fragments arranged by size:
3815 1280 1064 412 374 146 138 95
EcoICRI GAG'CTC
Cuts at: 2083 2083
Size: 7324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2976 3249 3312 3365 3509 3923
Size: 393 818 526 273 63 53 144 414
Cuts at: 3923 3932 5387 5984 6771 7039 1239
Size: 9 1455 597 787 268 1524
Fragments arranged by size:
1524 1455 818 787 597 526 414 393
273 268 144 63 53 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3098 3098
Size: 7324
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 7324
EcoRI G'AATT_C
Cuts at: 3286 4633 5011 3286
Size: 1347 378 5599
Fragments arranged by size:
5599 1347 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4133 4344 4615 4133
Size: 211 271 6842
Fragments arranged by size:
6842 271 211
FspI TGC'GCA
Cuts at: 5830 5830
Size: 7324
HaeII r_GCGC'y
Cuts at: 1678 6471 1678
Size: 4793 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2133 2165 2218 2250 2512
Size: 32 135 32 561 32 53 32 262
Cuts at: 2512 2544 3684 3716 3835 3867 5965 5997 6039
Size: 32 1140 32 119 32 2098 32 42
Cuts at: 6039 6071 1373
Size: 32 2626
Fragments arranged by size:
2626 2098 1140 561 262 135 119 53
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2334 4552 4668 4914 669
Size: 988 281 396 2218 116 246 3079
Fragments arranged by size:
3079 2218 988 396 281 246 116
HindIII A'AGCT_T
Cuts at: 748 3278 4341 748
Size: 2530 1063 3731
Fragments arranged by size:
3731 2530 1063
HpaI GTT'AAC
Cuts at: 2334 4914 2334
Size: 2580 4744
KpnI G_GTAC'C
Cuts at: 4649 4649
Size: 7324
MfeI C'AATT_G
Cuts at: 3722 4923 3722
Size: 1201 6123
MluI A'CGCG_T
Cuts at: 7200 7200
Size: 7324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4008 4068 6438 6622 1305
Size: 2703 60 2370 184 2007
Fragments arranged by size:
2703 2370 2007 184 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5502
Fragments arranged by size:
5502 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3602 5282 5641 5800 518
Size: 648 2436 1680 359 159 2042
Fragments arranged by size:
2436 2042 1680 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3470 4740 5417 6565 6810 1606
Size: 1864 1270 677 1148 245 2120
Fragments arranged by size:
2120 1864 1270 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6772
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7324
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7324
NotI GC'GGCC_GC
Cuts at: 4771 4771
Size: 7324
NsiI A_TGCA'T
Cuts at: 2010 2392 2461 3659 2010
Size: 382 69 1198 5675
Fragments arranged by size:
5675 1198 382 69
NspI r_CATG'y
Cuts at: 1132 1229 2312 3551 4682 6227 1132
Size: 97 1083 1239 1131 1545 2229
Fragments arranged by size:
2229 1545 1239 1131 1083 97
PasI CC'CwG_GG
Cuts at: 1348 2725 1348
Size: 1377 5947
PciI A'CATG_T
Cuts at: 3547 6223 3547
Size: 2676 4648
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7324
PfoI T'CCnGG_A
Cuts at: 4258 4258
Size: 7324
PmeI GTTT'AAAC
Cuts at: 4628 4628
Size: 7324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5393 5425 6906 6938 5393
Size: 32 1481 32 5779
Fragments arranged by size:
5779 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 7324
PshAI GACnn'nnGTC
Cuts at: 1613 2865 1613
Size: 1252 6072
PsiI TTA'TAA
Cuts at: 3024 3141 3672 4894 3024
Size: 117 531 1222 5454
Fragments arranged by size:
5454 1222 531 117
PstI C_TGCA'G
Cuts at: 830 2079 2367 3490 4676 830
Size: 1249 288 1123 1186 3478
Fragments arranged by size:
3478 1249 1186 1123 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7324
PvuII CAG'CTG
Cuts at: 3470 3470
Size: 7324
RsrII CG'GwC_CG
Cuts at: 2104 2104
Size: 7324
SacI G_AGCT'C
Cuts at: 2085 2085
Size: 7324
SalI G'TCGA_C
Cuts at: 1936 4666 1936
Size: 2730 4594
SbfI CC_TGCA'GG
Cuts at: 2079 4676 2079
Size: 2597 4727
ScaI AGT'ACT
Cuts at: 4525 5572 7284 4525
Size: 1047 1712 4565
Fragments arranged by size:
4565 1712 1047
SfcI C'TryA_G
Cuts at: 826 1045 2075 2363 3486 4672 5807 6488 6679
Size: 219 1030 288 1123 1186 1135 681 191
Cuts at: 6679 826
Size: 1471
Fragments arranged by size:
1471 1186 1135 1123 1030 681 288 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7324
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7324
SmaI CCC'GGG
Cuts at: 1872 4651 1872
Size: 2779 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 3004 5428 6329 6591 820
Size: 197 926 174 887 2424 901 262 1553
Fragments arranged by size:
2424 1553 926 901 887 262 197 174
SnaBI TAC'GTA
Cuts at: 493 5115 493
Size: 4622 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7324
SphI G_CATG'C
Cuts at: 1132 4682 1132
Size: 3550 3774
SspI AAT'ATT
Cuts at: 5 52 3818 5248 5
Size: 47 3766 1430 2081
Fragments arranged by size:
3766 2081 1430 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7324
StyI C'CwwG_G
Cuts at: 513 1065 2212 2541 3730 4763 513
Size: 552 1147 329 1189 1033 3074
Fragments arranged by size:
3074 1189 1147 1033 552 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5351 5368 5521 5706 6151 325
Size: 1255 3771 17 153 185 445 1498
Fragments arranged by size:
3771 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3035 4523 5570
Size: 274 80 33 51 1443 1058 1488 1047
Cuts at: 5570 7282 96
Size: 1712 138
Fragments arranged by size:
1712 1488 1443 1058 1047 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2208 2240 3075 3107 3118 3150 7284 7316 2208
Size: 32 835 32 11 32 4134 32 2216
Fragments arranged by size:
4134 2216 835 32 32 32 32 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7324
XbaI T'CTAG_A
Cuts at: 4660 4660
Size: 7324
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7324
XmaI C'CCGG_G
Cuts at: 1870 4649 1870
Size: 2779 4545
XmnI GAAnn'nnTTC
Cuts at: 2472 2643 2928 4116 4432 5453 2472
Size: 171 285 1188 316 1021 4343
Fragments arranged by size:
4343 1188 1021 316 285 171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5132 276
Size: 53 83 186 936 3598 2468
Fragments arranged by size:
3598 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI