Restriction map for
pFN21AA1611
(Circular) (Six-base) MAPSORT of: pFN21AA1611.seq Check: 9227 from: 1 to: 7159
pFN21AA1611 7159 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4969 278
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2633 4502 1937
Size: 696 1869 4594
Fragments arranged by size:
4594 1869 696
Acc65I G'GTAC_C
Cuts at: 4480 4480
Size: 7159
AclI AA'CG_TT
Cuts at: 1563 5286 5659 1563
Size: 3723 373 3063
Fragments arranged by size:
3723 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2953 3373 3541 4129 4381 5222 6606 6874
Size: 1321 420 168 588 252 841 1384 268
Cuts at: 6874 1632
Size: 1917
Fragments arranged by size:
1917 1384 1321 841 588 420 268 252
168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6962
AflIII A'CryG_T
Cuts at: 1203 6058 7035 1203
Size: 4855 977 1327
Fragments arranged by size:
4855 1327 977
AgeI A'CCGG_T
Cuts at: 5990 5990
Size: 7159
AhdI GACnn_n'nnGTC
Cuts at: 5888 5888
Size: 7159
AleI CACnn'nnGTG
Cuts at: 1166 2351 1166
Size: 1185 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7159
AlwNI CAG_nnn'CTG
Cuts at: 4902 6474 4902
Size: 1572 5587
ApaLI G'TGCA_C
Cuts at: 5216 6372 5216
Size: 1156 6003
ApoI r'AATT_y
Cuts at: 1493 2051 2670 2809 2835 3003 3145 3171 3481
Size: 558 619 139 26 168 142 26 310
Cuts at: 3481 3507 3675 3927 4263 4468 4694 4846 6955
Size: 26 168 252 336 205 226 152 2109
Cuts at: 6955 1493
Size: 1697
Fragments arranged by size:
2109 1697 619 558 336 310 252 226
205 168 168 152 142 139 26 26
26
AseI AT'TA_AT
Cuts at: 160 1032 5713 160
Size: 872 4681 1606
Fragments arranged by size:
4681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
AvaI C'yCGr_G
Cuts at: 1870 1943 4484 1870
Size: 73 2541 4545
Fragments arranged by size:
4545 2541 73
BamHI G'GATC_C
Cuts at: 4489 4856 4489
Size: 367 6792
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4480 5935 618
Size: 325 217 627 2693 1455 1842
Fragments arranged by size:
2693 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 4478 1391
Size: 811 2276 4072
Fragments arranged by size:
4072 2276 811
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2391 2959 3715 4051 4387 928
Size: 971 492 568 756 336 336 3700
Fragments arranged by size:
3700 971 756 568 492 336 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5350 5384 1903
Size: 34 3413 34 3678
Fragments arranged by size:
3678 3413 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5051 6261 5051
Size: 1210 5949
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7159
BfrBI ATG'CAT
Cuts at: 3397 3985 4435 3397
Size: 588 450 6121
Fragments arranged by size:
6121 588 450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5770 136
Size: 107 122 71 631 605 4098 1525
Fragments arranged by size:
4098 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7154 7154
Size: 7159
BlpI GC'TnA_GC
Cuts at: 4576 4576
Size: 7159
Bme1580I G_kGCm'C
Cuts at: 1790 5220 6376 1790
Size: 3430 1156 2573
Fragments arranged by size:
3430 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 6367
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4197 4281 4890
Size: 416 53 83 399 24 2780 84 609
Cuts at: 4890 5848 442
Size: 958 1753
Fragments arranged by size:
2780 1753 958 609 416 399 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3660 3660
Size: 7159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2949 3033 3117 3201 3285 3537 3621 3705
Size: 1710 84 84 84 84 252 84 84
Cuts at: 3705 3789 3873 3957 4041 4377 5819 1239
Size: 84 84 84 84 336 1442 2579
Fragments arranged by size:
2579 1710 1442 336 252 84 84 84
84 84 84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5284 6149 6447 5284
Size: 865 298 5996
Fragments arranged by size:
5996 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5822 882
Size: 617 4323 2219
Fragments arranged by size:
4323 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4950 493
Size: 711 3746 2702
Fragments arranged by size:
3746 2702 711
BsaBI GATnn'nnATC
Cuts at: 7153 7153
Size: 7159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4966 5348 5983 275
Size: 53 83 186 936 3433 382 635 1451
Fragments arranged by size:
3433 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4103 4439 5592 5990 6264 6411 6822 4103
Size: 336 1153 398 274 147 411 4440
Fragments arranged by size:
4440 1153 411 398 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6934
Fragments arranged by size:
6934 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 6267
BseYI C'CCAG_C
Cuts at: 6362 6858 6362
Size: 496 6663
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6586
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4609 5370 6398 1540
Size: 275 186 2608 761 1028 2301
Fragments arranged by size:
2608 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5220 5305 6376 5220
Size: 85 1071 6003
Fragments arranged by size:
6003 1071 85
BsmI GAATG_Cn'
Cuts at: 3397 3464 3884 3985 4437 4670 4763 3397
Size: 67 420 101 452 233 93 5793
Fragments arranged by size:
5793 452 420 233 101 93 67
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 4478 5220 5305 6376 1391
Size: 399 412 2276 742 85 1071 2174
Fragments arranged by size:
2276 2174 1071 742 412 399 85
BspEI T'CCGG_A
Cuts at: 6822 6822
Size: 7159
BspHI T'CATG_A
Cuts at: 2710 2950 3874 5048 6778 2710
Size: 240 924 1174 1730 3091
Fragments arranged by size:
3091 1730 1174 924 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4514 844
Size: 3670 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5046 1394
Size: 429 3223 3507
Fragments arranged by size:
3507 3223 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3208 3460 4132 5654 5828 65
Size: 1183 1960 252 672 1522 174 1396
Fragments arranged by size:
1960 1522 1396 1183 672 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5803 5990 1700
Size: 110 3993 187 2869
Fragments arranged by size:
3993 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2291 4292 96
Size: 2195 2001 2963
Fragments arranged by size:
2963 2195 2001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7159
BssSI C'ACGA_G
Cuts at: 5219 6231 5219
Size: 1012 6147
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2871 3207 3459 794
Size: 2077 336 252 4494
Fragments arranged by size:
4494 2077 336 252
BstBI TT'CG_AA
Cuts at: 2813 5963 2813
Size: 3150 4009
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7159
BstYI r'GATC_y
Cuts at: 1972 2094 4489 4856 5241 5258 6710 6796 6808
Size: 122 2395 367 385 17 1452 86 12
Cuts at: 6808 7154 1972
Size: 346 1977
Fragments arranged by size:
2395 1977 1452 385 367 346 122 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5668
Fragments arranged by size:
5668 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6420
Fragments arranged by size:
6420 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 4675 5469 5489 2872
Size: 1803 794 20 4542
Fragments arranged by size:
4542 1803 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4114 4149 529
Size: 35 3550 35 3539
Fragments arranged by size:
3550 3539 35 35
DraI TTT'AAA
Cuts at: 4463 4463
Size: 7159
DraIII CAC_nnn'GTG
Cuts at: 2350 2350
Size: 7159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6166 809
Size: 507 4850 1802
Fragments arranged by size:
4850 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 4606 5495
Size: 54 1425 50 275 18 213 2563 889
Cuts at: 5495 7124 8
Size: 1629 43
Fragments arranged by size:
2563 1629 1425 889 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4606 1537
Size: 275 2794 4090
Fragments arranged by size:
4090 2794 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2315 2506 3140 5089 1353
Size: 854 108 191 634 1949 3423
Fragments arranged by size:
3423 1949 854 634 191 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5746 6120 6266 387
Size: 1064 138 4157 374 146 1280
Fragments arranged by size:
4157 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2949 2953 3033 3117 3201 3285 3373
Size: 393 1317 4 80 84 84 84 88
Cuts at: 3373 3537 3541 3621 3705 3789 3873 3957 4041
Size: 164 4 80 84 84 84 84 84
Cuts at: 4041 4129 4377 4381 5222 5819 6606 6874 1239
Size: 88 248 4 841 597 787 268 1524
Fragments arranged by size:
1524 1317 841 787 597 393 268 248
164 88 88 84 84 84 84 84
84 84 84 80 80 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2015
Size: 7159
EcoRI G'AATT_C
Cuts at: 2051 2809 2835 3003 3171 3507 3675 3927 4263
Size: 758 26 168 168 336 168 252 336
Cuts at: 4263 4468 4846 2051
Size: 205 378 4364
Fragments arranged by size:
4364 758 378 336 336 252 205 168
168 168 26
EcoRV GAT'ATC
Cuts at: 2278 2278
Size: 7159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3846 3846
Size: 7159
FspI TGC'GCA
Cuts at: 5665 5665
Size: 7159
HaeII r_GCGC'y
Cuts at: 1678 6306 1678
Size: 4628 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3568 3600 4396 4428 5800
Size: 32 135 32 1996 32 796 32 1372
Cuts at: 5800 5832 5874 5906 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1996 1372 796 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 4503 4749 669
Size: 988 281 90 2475 246 3079
Fragments arranged by size:
3079 2475 988 281 246 90
HindIII A'AGCT_T
Cuts at: 748 2528 2554 748
Size: 1780 26 5353
Fragments arranged by size:
5353 1780 26
HpaI GTT'AAC
Cuts at: 4749 4749
Size: 7159
KpnI G_GTAC'C
Cuts at: 4484 4484
Size: 7159
MfeI C'AATT_G
Cuts at: 4758 4758
Size: 7159
MluI A'CGCG_T
Cuts at: 7035 7035
Size: 7159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 2163 2337 6273 6457 1305
Size: 792 66 174 3936 184 2007
Fragments arranged by size:
3936 2007 792 184 174 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 10
Size: 54 1425 343 213 5124
Fragments arranged by size:
5124 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2955 3348 5117 5476 5635 518
Size: 648 1185 604 393 1769 359 159 2042
Fragments arranged by size:
2042 1769 1185 648 604 393 359 159
MspA1I CmG'CkG
Cuts at: 1606 4575 5252 6400 6645 1606
Size: 2969 677 1148 245 2120
Fragments arranged by size:
2969 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5668
Fragments arranged by size:
5668 939 552
NdeI CA'TA_TG
Cuts at: 387 4341 387
Size: 3954 3205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7159
NotI GC'GGCC_GC
Cuts at: 4606 4606
Size: 7159
NsiI A_TGCA'T
Cuts at: 3399 3987 4437 3399
Size: 588 450 6121
Fragments arranged by size:
6121 588 450
NspI r_CATG'y
Cuts at: 1132 1229 4517 6062 1132
Size: 97 3288 1545 2229
Fragments arranged by size:
3288 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7159
PciI A'CATG_T
Cuts at: 6058 6058
Size: 7159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 1108
Size: 1242 5917
PmeI GTTT'AAAC
Cuts at: 4463 4463
Size: 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5228 5260 6741 6773 5228
Size: 32 1481 32 5614
Fragments arranged by size:
5614 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7159
PsiI TTA'TAA
Cuts at: 2775 3158 4729 2775
Size: 383 1571 5205
Fragments arranged by size:
5205 1571 383
PstI C_TGCA'G
Cuts at: 830 2583 4511 830
Size: 1753 1928 3478
Fragments arranged by size:
3478 1928 1753
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7159
SalI G'TCGA_C
Cuts at: 1936 4501 1936
Size: 2565 4594
SapI GCTCTTCn'nnn_
Cuts at: 2207 2315 3140 2207
Size: 108 825 6226
Fragments arranged by size:
6226 825 108
SbfI CC_TGCA'GG
Cuts at: 4511 4511
Size: 7159
ScaI AGT'ACT
Cuts at: 5407 7119 5407
Size: 1712 5447
SfcI C'TryA_G
Cuts at: 826 1045 2579 4507 5642 6323 6514 826
Size: 219 1534 1928 1135 681 191 1471
Fragments arranged by size:
1928 1534 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7159
SmaI CCC'GGG
Cuts at: 1872 4486 1872
Size: 2614 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5263 6164 6426 820
Size: 197 926 3320 901 262 1553
Fragments arranged by size:
3320 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4950 493
Size: 4457 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7159
SphI G_CATG'C
Cuts at: 1132 4517 1132
Size: 3385 3774
SspI AAT'ATT
Cuts at: 5 52 2170 5083 5
Size: 47 2118 2913 2081
Fragments arranged by size:
2913 2118 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7159
StyI C'CwwG_G
Cuts at: 513 1065 2004 4598 513
Size: 552 939 2594 3074
Fragments arranged by size:
3074 2594 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4184 5186 5203 5356 5541 5986 325
Size: 1255 2604 1002 17 153 185 445 1498
Fragments arranged by size:
2604 1498 1255 1002 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2291 2327 4292
Size: 274 80 33 51 1443 314 36 1965
Cuts at: 4292 5405 7117 96
Size: 1113 1712 138
Fragments arranged by size:
1965 1712 1443 1113 314 274 138 80
51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7119 7151 7119
Size: 32 7127
Tth111I GACn'n_nGTC
Cuts at: 1316 2151 1316
Size: 835 6324
XbaI T'CTAG_A
Cuts at: 4495 4495
Size: 7159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7159
XmaI C'CCGG_G
Cuts at: 1870 4484 1870
Size: 2614 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 3679 4142 5288 3554
Size: 125 463 1146 5425
Fragments arranged by size:
5425 1146 463 125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4967 276
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI