Restriction map for
pFN21AA1598
(Circular) (Six-base) MAPSORT of: pFN21AA1598.seq Check: 6776 from: 1 to: 6598
pFN21AA1598 6598 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3941 1937
Size: 2004 4594
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 2291 4725 5098 1563
Size: 728 2434 373 3063
Fragments arranged by size:
3063 2434 728 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2274 2367 3006 3222 4661 6045 6313
Size: 440 202 93 639 216 1439 1384 268
Cuts at: 6313 1632
Size: 1917
Fragments arranged by size:
1917 1439 1384 639 440 268 216 202
93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 5497 6474 1203
Size: 4294 977 1327
Fragments arranged by size:
4294 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 3153 1166
Size: 1987 4611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6598
AlwNI CAG_nnn'CTG
Cuts at: 2932 4341 5913 2932
Size: 1409 1572 3617
Fragments arranged by size:
3617 1572 1409
ApaI G_GGCC'C
Cuts at: 3636 3636
Size: 6598
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 2165 2175 2572 2844 2980 3001 3052 3590
Size: 672 10 397 272 136 21 51 538
Cuts at: 3590 3834 3907 4133 4285 6394 1493
Size: 244 73 226 152 2109 1697
Fragments arranged by size:
2109 1697 672 538 397 272 244 226
152 136 73 51 21 10
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 3923 1870
Size: 73 1980 4545
Fragments arranged by size:
4545 1980 73
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2393 3919 5374 618
Size: 325 217 627 606 1526 1455 1842
Fragments arranged by size:
1842 1526 1455 627 606 325 217
BanII G_rGCy'C
Cuts at: 1391 2771 3636 3917 1391
Size: 1380 865 281 4072
Fragments arranged by size:
4072 1380 865 281
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 2240 3308 3861 928
Size: 971 186 155 1068 553 3665
Fragments arranged by size:
3665 1068 971 553 186 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4789 4823 1903
Size: 34 2852 34 3678
Fragments arranged by size:
3678 2852 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3553 4490 5700 3553
Size: 937 1210 4451
Fragments arranged by size:
4451 1210 937
BclI T'GATC_A
Cuts at: 1548 2779 1548
Size: 1231 5367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5209 136
Size: 107 122 71 631 605 3537 1525
Fragments arranged by size:
3537 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3214 6593 3214
Size: 3379 3219
BlpI GC'TnA_GC
Cuts at: 4015 4015
Size: 6598
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4659 5815 1790
Size: 1846 1023 1156 2573
Fragments arranged by size:
2573 1846 1156 1023
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 3371 3501
Size: 416 53 83 399 24 1730 224 130
Cuts at: 3501 3678 4329 5287 442
Size: 177 651 958 1753
Fragments arranged by size:
1753 1730 958 651 416 399 224 177
130 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2687 2693 2954 3011 3602 5258 1239
Size: 1448 6 261 57 591 1656 2579
Fragments arranged by size:
2579 1656 1448 591 261 57 6
Bpu10I CC'TnA_GC
Cuts at: 3618 3618
Size: 6598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 4723 5588 5886 2492
Size: 2231 865 298 3204
Fragments arranged by size:
3204 2231 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5261 882
Size: 617 3762 2219
Fragments arranged by size:
3762 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4389 493
Size: 711 3185 2702
Fragments arranged by size:
3185 2702 711
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4405 4787 5422 275
Size: 53 83 186 936 2872 382 635 1451
Fragments arranged by size:
2872 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3135 5031 5429 5703 5850 6261 3135
Size: 1896 398 274 147 411 3472
Fragments arranged by size:
3472 1896 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6373
Fragments arranged by size:
6373 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2663 3063 3066 1186
Size: 1477 400 3 4718
Fragments arranged by size:
4718 1477 400 3
BseYI C'CCAG_C
Cuts at: 2326 2823 5801 6297 2326
Size: 497 2978 496 2627
Fragments arranged by size:
2978 2627 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3357 1163
Size: 573 1621 4404
Fragments arranged by size:
4404 1621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4048 4809 5837 1540
Size: 275 186 2047 761 1028 2301
Fragments arranged by size:
2301 2047 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2771 4659 4744 5815 2771
Size: 1888 85 1071 3554
Fragments arranged by size:
3554 1888 1071 85
BsmI GAATG_Cn'
Cuts at: 4109 4202 4109
Size: 93 6505
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2771 3636 3917 4659 4744 5815 1391
Size: 399 981 865 281 742 85 1071 2174
Fragments arranged by size:
2174 1071 981 865 742 399 281 85
BspEI T'CCGG_A
Cuts at: 3135 6261 3135
Size: 3126 3472
BspHI T'CATG_A
Cuts at: 4487 6217 4487
Size: 1730 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3953 844
Size: 3109 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4485 1394
Size: 429 2662 3507
Fragments arranged by size:
3507 2662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5093 5267 65
Size: 1183 3845 174 1396
Fragments arranged by size:
3845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5242 5429 1700
Size: 110 3432 187 2869
Fragments arranged by size:
3432 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2313 96
Size: 2217 4381
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 4658 5670 4658
Size: 1012 5586
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3696 794
Size: 2902 3696
BstBI TT'CG_AA
Cuts at: 2686 3320 5402 2686
Size: 634 2082 3882
Fragments arranged by size:
3882 2082 634
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 3155 2497
Size: 658 5940
BstYI r'GATC_y
Cuts at: 1972 3214 3751 3802 3928 4295 4680 4697 6149
Size: 1242 537 51 126 367 385 17 1452
Cuts at: 6149 6235 6247 6593 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1242 537 385 367 346 126
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5859
Fragments arranged by size:
5859 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3345 4114 4908 4928 3166
Size: 179 769 794 20 4836
Fragments arranged by size:
4836 794 769 179 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 2571 3006 3902 2571
Size: 435 896 5267
Fragments arranged by size:
5267 896 435
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 4045 4934
Size: 54 1425 50 275 18 489 1726 889
Cuts at: 4934 6563 8
Size: 1629 43
Fragments arranged by size:
1726 1629 1425 889 489 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 1537
Size: 275 2233 4090
Fragments arranged by size:
4090 2233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2343 2634 2832 2871 2982 3231 4528
Size: 663 327 291 198 39 111 249 1297
Cuts at: 4528 1353
Size: 3423
Fragments arranged by size:
3423 1297 663 327 291 249 198 111
39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5185 5559 5705 387
Size: 1064 138 3596 374 146 1280
Fragments arranged by size:
3596 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2769 2769
Size: 6598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2274 2367 2687 2693 2954 3006
Size: 393 440 202 93 320 6 261 52
Cuts at: 3006 3011 3222 3602 4661 5258 6045 6313 1239
Size: 5 211 380 1059 597 787 268 1524
Fragments arranged by size:
1524 1059 787 597 440 393 380 320
268 261 211 202 93 52 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3621 3621
Size: 6598
EcoO109I rG'GnC_Cy
Cuts at: 2680 3632 3633 2680
Size: 952 1 5645
Fragments arranged by size:
5645 952 1
EcoRI G'AATT_C
Cuts at: 2844 2980 3052 3590 3907 4285 2844
Size: 136 72 538 317 378 5157
Fragments arranged by size:
5157 538 378 317 136 72
FspI TGC'GCA
Cuts at: 5104 5104
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 5745 1678
Size: 4067 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3433 3465 3503 3535 5239
Size: 32 135 32 1861 32 38 32 1704
Cuts at: 5239 5271 5313 5345 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1861 1704 135 42 38 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3942 4188 669
Size: 988 281 2004 246 3079
Fragments arranged by size:
3079 2004 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2256 2796 3394 748
Size: 1508 540 598 3952
Fragments arranged by size:
3952 1508 598 540
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3512 3830 3915 5712 5896 1305
Size: 2207 318 85 1797 184 2007
Fragments arranged by size:
2207 2007 1797 318 184 85
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 10
Size: 54 1425 343 489 4287
Fragments arranged by size:
4287 1425 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3128 3153 3527 4556 4915 5074 518
Size: 648 1962 25 374 1029 359 159 2042
Fragments arranged by size:
2042 1962 1029 648 374 359 159 25
MspA1I CmG'CkG
Cuts at: 1606 2032 4014 4691 5839 6084 1606
Size: 426 1982 677 1148 245 2120
Fragments arranged by size:
2120 1982 1148 677 426 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6046
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 2311 3956 5501 1132
Size: 97 1082 1645 1545 2229
Fragments arranged by size:
2229 1645 1545 1082 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6598
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6598
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2680 2680
Size: 6598
PshAI GACnn'nnGTC
Cuts at: 1613 2408 1613
Size: 795 5803
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspOMI G'GGCC_C
Cuts at: 3632 3632
Size: 6598
PstI C_TGCA'G
Cuts at: 830 2037 2766 3950 830
Size: 1207 729 1184 3478
Fragments arranged by size:
3478 1207 1184 729
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
PvuII CAG'CTG
Cuts at: 2032 2032
Size: 6598
SacI G_AGCT'C
Cuts at: 2771 2771
Size: 6598
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SapI GCTCTTCn'nnn_
Cuts at: 2871 2871
Size: 6598
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SexAI A'CCwGG_T
Cuts at: 3786 3786
Size: 6598
SfcI C'TryA_G
Cuts at: 826 1045 2033 2379 2634 2762 3946 5081 5762
Size: 219 988 346 255 128 1184 1135 681
Cuts at: 5762 5953 826
Size: 191 1471
Fragments arranged by size:
1471 1184 1135 988 681 346 255 219
191 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 3925 1872
Size: 2053 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 4702 5603 5865 820
Size: 197 926 564 2195 901 262 1553
Fragments arranged by size:
2195 1553 926 901 564 262 197
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 3383 152
Size: 3231 3367
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SspI AAT'ATT
Cuts at: 5 52 4522 5
Size: 47 4470 2081
Fragments arranged by size:
4470 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6598
StyI C'CwwG_G
Cuts at: 513 1065 3663 4037 513
Size: 552 2598 374 3074
Fragments arranged by size:
3074 2598 552 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2837 3313 4625 4642 4795 4980 5425
Size: 1255 1257 476 1312 17 153 185 445
Cuts at: 5425 325
Size: 1498
Fragments arranged by size:
1498 1312 1257 1255 476 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2313 2416 4844
Size: 274 80 33 51 1443 336 103 2428
Cuts at: 4844 6556 96
Size: 1712 138
Fragments arranged by size:
2428 1712 1443 336 274 138 103 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6558 6590 6558
Size: 32 6566
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6598
XbaI T'CTAG_A
Cuts at: 2941 3934 2941
Size: 993 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3529 3529
Size: 6598
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6598
XmaI C'CCGG_G
Cuts at: 1870 3923 1870
Size: 2053 4545
XmnI GAAnn'nnTTC
Cuts at: 2166 4727 2166
Size: 2561 4037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI