Restriction map for
pFN21AA1597
(Circular) (Six-base) MAPSORT of: pFN21AA1597.seq Check: 902 from: 1 to: 7390
pFN21AA1597 7390 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5200 278
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4733 1937
Size: 2796 4594
Acc65I G'GTAC_C
Cuts at: 4143 4711 4143
Size: 568 6822
AclI AA'CG_TT
Cuts at: 1563 5517 5890 1563
Size: 3954 373 3063
Fragments arranged by size:
3954 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2117 3588 3833 4172 4470 4572 4584
Size: 422 63 1471 245 339 298 102 12
Cuts at: 4584 5453 6837 7105 1632
Size: 869 1384 268 1917
Fragments arranged by size:
1917 1471 1384 869 422 339 298 268
245 102 63 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7193
AflIII A'CryG_T
Cuts at: 1203 6289 7266 1203
Size: 5086 977 1327
Fragments arranged by size:
5086 1327 977
AgeI A'CCGG_T
Cuts at: 6221 6221
Size: 7390
AhdI GACnn_n'nnGTC
Cuts at: 6119 6119
Size: 7390
AleI CACnn'nnGTG
Cuts at: 1166 4427 1166
Size: 3261 4129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2040 1184
Size: 856 6534
AlwNI CAG_nnn'CTG
Cuts at: 2095 4238 5133 6705 2095
Size: 2143 895 1572 2780
Fragments arranged by size:
2780 2143 1572 895
ApaLI G'TGCA_C
Cuts at: 4268 5447 6603 4268
Size: 1179 1156 5055
Fragments arranged by size:
5055 1179 1156
ApoI r'AATT_y
Cuts at: 1493 2886 3023 3265 3621 4324 4699 4925 5077
Size: 1393 137 242 356 703 375 226 152
Cuts at: 5077 7186 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1393 703 375 356 242 226
152 137
AseI AT'TA_AT
Cuts at: 160 1032 3210 5944 160
Size: 872 2178 2734 1606
Fragments arranged by size:
2734 2178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
AvaI C'yCGr_G
Cuts at: 1870 1943 2344 4715 1870
Size: 73 401 2371 4545
Fragments arranged by size:
4545 2371 401 73
AvrII C'CTAG_G
Cuts at: 4075 4075
Size: 7390
BamHI G'GATC_C
Cuts at: 2772 4720 5087 2772
Size: 1948 367 5075
Fragments arranged by size:
5075 1948 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3385 4143 4711 6166 618
Size: 325 217 627 1598 758 568 1455 1842
Fragments arranged by size:
1842 1598 1455 758 627 568 325 217
BanII G_rGCy'C
Cuts at: 1391 2764 4709 1391
Size: 1373 1945 4072
Fragments arranged by size:
4072 1945 1373
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2919 3504 4166 4385 4517 928
Size: 971 1020 585 662 219 132 3801
Fragments arranged by size:
3801 1020 971 662 585 219 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5581 5615 1903
Size: 34 3644 34 3678
Fragments arranged by size:
3678 3644 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4045 5282 6492 4045
Size: 1237 1210 4943
Fragments arranged by size:
4943 1237 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7390
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6001 136
Size: 107 122 71 631 605 4329 1525
Fragments arranged by size:
4329 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4453 7385 4453
Size: 2932 4458
BlpI GC'TnA_GC
Cuts at: 2214 4807 2214
Size: 2593 4797
Bme1580I G_kGCm'C
Cuts at: 1790 4272 5451 6607 1790
Size: 2482 1179 1156 2573
Fragments arranged by size:
2573 2482 1179 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 3832 4120
Size: 416 53 83 399 24 2012 403 288
Cuts at: 4120 5121 6079 442
Size: 1001 958 1753
Fragments arranged by size:
2012 1753 1001 958 416 403 399 288
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2778 2792 2778
Size: 14 7376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2274 2406 2773 2957 4199 4281 6050 1239
Size: 1035 132 367 184 1242 82 1769 2579
Fragments arranged by size:
2579 1769 1242 1035 367 184 132 82
Bpu10I CC'TnA_GC
Cuts at: 3102 3372 4307 3102
Size: 270 935 6185
Fragments arranged by size:
6185 935 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 3133 5515 6380 6678 2762
Size: 371 2382 865 298 3474
Fragments arranged by size:
3474 2382 865 371 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3399 6053 882
Size: 617 1900 2654 2219
Fragments arranged by size:
2654 2219 1900 617
BsaAI yAC'GTr
Cuts at: 493 1204 5181 493
Size: 711 3977 2702
Fragments arranged by size:
3977 2702 711
BsaBI GATnn'nnATC
Cuts at: 7384 7384
Size: 7390
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5197 5579 6214 275
Size: 53 83 186 936 3664 382 635 1451
Fragments arranged by size:
3664 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5823 6221 6495 6642 7053 5823
Size: 398 274 147 411 6160
Fragments arranged by size:
6160 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4197 4227 1178
Size: 30 165 30 2794 30 4341
Fragments arranged by size:
4341 2794 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7390
BseYI C'CCAG_C
Cuts at: 2253 6593 7089 2253
Size: 4340 496 2554
Fragments arranged by size:
4340 2554 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6817
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4840 5601 6629 1540
Size: 275 186 2839 761 1028 2301
Fragments arranged by size:
2839 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4272 5451 5536 6607 4272
Size: 1179 85 1071 5055
Fragments arranged by size:
5055 1179 1071 85
BsmI GAATG_Cn'
Cuts at: 2902 3220 3614 4292 4668 4901 4994 2902
Size: 318 394 678 376 233 93 5298
Fragments arranged by size:
5298 678 394 376 318 233 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2764 4272 4709 5451 5536 6607 1391
Size: 399 974 1508 437 742 85 1071 2174
Fragments arranged by size:
2174 1508 1071 974 742 437 399 85
BspEI T'CCGG_A
Cuts at: 7053 7053
Size: 7390
BspHI T'CATG_A
Cuts at: 5279 7009 5279
Size: 1730 5660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4745 844
Size: 3901 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5277 1394
Size: 429 3454 3507
Fragments arranged by size:
3507 3454 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5885 6059 65
Size: 1183 4637 174 1396
Fragments arranged by size:
4637 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6034 6221 1700
Size: 110 4224 187 2869
Fragments arranged by size:
4224 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7390
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7390
BssSI C'ACGA_G
Cuts at: 5450 6462 5450
Size: 1012 6378
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2620 4172 794
Size: 1826 1552 4012
Fragments arranged by size:
4012 1826 1552
BstBI TT'CG_AA
Cuts at: 6194 6194
Size: 7390
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7390
BstYI r'GATC_y
Cuts at: 1972 2772 4453 4720 5087 5472 5489 6941 7027
Size: 800 1681 267 367 385 17 1452 86
Cuts at: 7027 7039 7385 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1681 1452 800 385 367 346 267
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2158 3004 513
Size: 552 1093 846 4899
Fragments arranged by size:
4899 1093 846 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6651
Fragments arranged by size:
6651 683 56
BtsI GCAGTG_nn'
Cuts at: 2973 3733 4301 4649 4906 5700 5720 2973
Size: 760 568 348 257 794 20 4643
Fragments arranged by size:
4643 794 760 568 348 257 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7355
DraI TTT'AAA
Cuts at: 2700 4694 2700
Size: 1994 5396
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6397 809
Size: 507 5081 1802
Fragments arranged by size:
5081 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2110 4837 5726
Size: 54 1425 50 275 18 280 2727 889
Cuts at: 5726 7355 8
Size: 1629 43
Fragments arranged by size:
2727 1629 1425 889 280 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4837 1537
Size: 275 3025 4090
Fragments arranged by size:
4090 3025 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2043 2189 2307 2316 2423 2926 3078
Size: 677 13 146 118 9 107 503 152
Cuts at: 3078 5320 1353
Size: 2242 3423
Fragments arranged by size:
3423 2242 677 503 152 146 118 107
13 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5977 6351 6497 387
Size: 1064 138 4388 374 146 1280
Fragments arranged by size:
4388 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2117 2274 2406 2773 2957 3588
Size: 393 422 63 157 132 367 184 631
Cuts at: 3588 3833 4172 4199 4281 4470 4572 4584 5453
Size: 245 339 27 82 189 102 12 869
Cuts at: 5453 6050 6837 7105 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 869 787 631 597 422 393 367
339 268 245 189 184 157 132 102
82 63 27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4079 4079
Size: 7390
EcoO109I rG'GnC_Cy
Cuts at: 2199 2640 2199
Size: 441 6949
EcoRI G'AATT_C
Cuts at: 3265 4699 5077 3265
Size: 1434 378 5578
Fragments arranged by size:
5578 1434 378
EcoRV GAT'ATC
Cuts at: 2170 2170
Size: 7390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3976 3976
Size: 7390
FspI TGC'GCA
Cuts at: 5896 5896
Size: 7390
HaeII r_GCGC'y
Cuts at: 1678 6537 1678
Size: 4859 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2422 2454 2534 2566 2702
Size: 32 135 32 850 32 80 32 136
Cuts at: 2702 2734 3284 3316 3338 3370 3491 3523 6031
Size: 32 550 32 22 32 121 32 2508
Cuts at: 6031 6063 6105 6137 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2508 850 550 136 135 121 80
42 32 32 32 32 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 2571 4734 4980 669
Size: 988 281 331 302 2163 246 3079
Fragments arranged by size:
3079 2163 988 331 302 281 246
HindIII A'AGCT_T
Cuts at: 748 4247 748
Size: 3499 3891
HpaI GTT'AAC
Cuts at: 4980 4980
Size: 7390
KpnI G_GTAC'C
Cuts at: 4147 4715 4147
Size: 568 6822
MfeI C'AATT_G
Cuts at: 3792 4133 4989 3792
Size: 341 856 6193
Fragments arranged by size:
6193 856 341
MluI A'CGCG_T
Cuts at: 7266 7266
Size: 7390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6504 6688 1305
Size: 5199 184 2007
Fragments arranged by size:
5199 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2112 10
Size: 54 1425 343 280 5288
Fragments arranged by size:
5288 1425 343 280 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4277 4427 5348 5707 5866 518
Size: 648 3111 150 921 359 159 2042
Fragments arranged by size:
3111 2042 921 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2020 2095 2213 3860 4806 5483 6631 6876
Size: 414 75 118 1647 946 677 1148 245
Cuts at: 6876 1606
Size: 2120
Fragments arranged by size:
2120 1647 1148 946 677 414 245 118
75
NcoI C'CATG_G
Cuts at: 513 1065 2158 3004 513
Size: 552 1093 846 4899
Fragments arranged by size:
4899 1093 846 552
NdeI CA'TA_TG
Cuts at: 387 3157 3889 387
Size: 2770 732 3888
Fragments arranged by size:
3888 2770 732
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7390
NotI GC'GGCC_GC
Cuts at: 4837 4837
Size: 7390
NspI r_CATG'y
Cuts at: 1132 1229 2954 3827 4748 6293 1132
Size: 97 1725 873 921 1545 2229
Fragments arranged by size:
2229 1725 1545 921 873 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7390
PciI A'CATG_T
Cuts at: 6289 6289
Size: 7390
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3212 4238 4426 4516 4635 1108
Size: 2104 1026 188 90 119 3863
Fragments arranged by size:
3863 2104 1026 188 119 90
PmeI GTTT'AAAC
Cuts at: 4694 4694
Size: 7390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5459 5491 6972 7004 5459
Size: 32 1481 32 5845
Fragments arranged by size:
5845 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2640 2640
Size: 7390
PshAI GACnn'nnGTC
Cuts at: 1613 3253 1613
Size: 1640 5750
PsiI TTA'TAA
Cuts at: 2826 4960 2826
Size: 2134 5256
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 7358
PstI C_TGCA'G
Cuts at: 830 2968 4742 830
Size: 2138 1774 3478
Fragments arranged by size:
3478 2138 1774
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7390
PvuII CAG'CTG
Cuts at: 2095 2213 2095
Size: 118 7272
SalI G'TCGA_C
Cuts at: 1936 4732 1936
Size: 2796 4594
SanDI GG'GwC_CC
Cuts at: 2640 2640
Size: 7390
SapI GCTCTTCn'nnn_
Cuts at: 2307 2307
Size: 7390
SbfI CC_TGCA'GG
Cuts at: 4742 4742
Size: 7390
ScaI AGT'ACT
Cuts at: 5638 7350 5638
Size: 1712 5678
SfcI C'TryA_G
Cuts at: 826 1045 2964 4389 4738 5873 6554 6745 826
Size: 219 1919 1425 349 1135 681 191 1471
Fragments arranged by size:
1919 1471 1425 1135 681 349 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7390
SmaI CCC'GGG
Cuts at: 1872 4717 1872
Size: 2845 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2777 3148 5494 6395 6657 820
Size: 197 926 834 371 2346 901 262 1553
Fragments arranged by size:
2346 1553 926 901 834 371 262 197
SnaBI TAC'GTA
Cuts at: 493 5181 493
Size: 4688 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7390
SphI G_CATG'C
Cuts at: 1132 4748 1132
Size: 3616 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5314 5
Size: 47 3729 1533 2081
Fragments arranged by size:
3729 2081 1533 47
StuI AGG'CCT
Cuts at: 1517 2628 3899 4447 1517
Size: 1111 1271 548 4460
Fragments arranged by size:
4460 1271 1111 548
StyI C'CwwG_G
Cuts at: 513 1065 2130 2158 3004 4075 4829 513
Size: 552 1065 28 846 1071 754 3074
Fragments arranged by size:
3074 1071 1065 846 754 552 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 4267 5417 5434 5587 5772 6217
Size: 1255 665 2022 1150 17 153 185 445
Cuts at: 6217 325
Size: 1498
Fragments arranged by size:
2022 1498 1255 1150 665 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5636 7348 96
Size: 274 80 33 51 1443 3659 1712 138
Fragments arranged by size:
3659 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7350 7382 7350
Size: 32 7358
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7390
XbaI T'CTAG_A
Cuts at: 3401 3493 4726 3401
Size: 92 1233 6065
Fragments arranged by size:
6065 1233 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4513 4513
Size: 7390
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7390
XmaI C'CCGG_G
Cuts at: 1870 4715 1870
Size: 2845 4545
XmnI GAAnn'nnTTC
Cuts at: 2817 3087 3124 5519 2817
Size: 270 37 2395 4688
Fragments arranged by size:
4688 2395 270 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5198 276
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII
SexAI SfiI SfoI SrfI SwaI