Restriction map for
pFN21AA1594
(Circular) (Six-base) MAPSORT of: pFN21AA1594.seq Check: 6545 from: 1 to: 7642
pFN21AA1594 7642 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4985 1937
Size: 3048 4594
Acc65I G'GTAC_C
Cuts at: 4963 4963
Size: 7642
AclI AA'CG_TT
Cuts at: 1563 3976 5769 6142 1563
Size: 2413 1793 373 3063
Fragments arranged by size:
3063 2413 1793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 4421 4638 4698 5705 7089 7357 1632
Size: 404 2385 217 60 1007 1384 268 1917
Fragments arranged by size:
2385 1917 1384 1007 404 268 217 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 6541 7518 1203
Size: 5338 977 1327
Fragments arranged by size:
5338 1327 977
AgeI A'CCGG_T
Cuts at: 6473 6473
Size: 7642
AhdI GACnn_n'nnGTC
Cuts at: 4929 6371 4929
Size: 1442 6200
AleI CACnn'nnGTG
Cuts at: 1166 4913 1166
Size: 3747 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3303 3436 1184
Size: 2119 133 5390
Fragments arranged by size:
5390 2119 133
AlwNI CAG_nnn'CTG
Cuts at: 3022 4447 4616 5385 6957 3022
Size: 1425 169 769 1572 3707
Fragments arranged by size:
3707 1572 1425 769 169
ApaLI G'TGCA_C
Cuts at: 5699 6855 5699
Size: 1156 6486
ApoI r'AATT_y
Cuts at: 1493 2037 2142 3138 3296 3385 3656 3871 3908
Size: 544 105 996 158 89 271 215 37
Cuts at: 3908 4951 5177 5329 7438 1493
Size: 1043 226 152 2109 1697
Fragments arranged by size:
2109 1697 1043 996 544 271 226 215
158 152 105 89 37
AseI AT'TA_AT
Cuts at: 160 1032 6196 160
Size: 872 5164 1606
Fragments arranged by size:
5164 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 4967 1870
Size: 73 3024 4545
Fragments arranged by size:
4545 3024 73
AvrII C'CTAG_G
Cuts at: 3671 3671
Size: 7642
BamHI G'GATC_C
Cuts at: 2078 2828 4303 4972 5339 2078
Size: 750 1475 669 367 4381
Fragments arranged by size:
4381 1475 750 669 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4963 6418 618
Size: 325 217 627 3176 1455 1842
Fragments arranged by size:
3176 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3932 4961 1391
Size: 2541 1029 4072
Fragments arranged by size:
4072 2541 1029
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3365 4041 4517 4928 928
Size: 971 1466 676 476 411 3642
Fragments arranged by size:
3642 1466 971 676 476 411
BbvCI CC'TCA_GC
Cuts at: 2922 3989 4623 2922
Size: 1067 634 5941
Fragments arranged by size:
5941 1067 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4799 4833 5833 5867 1903
Size: 34 2862 34 1000 34 3678
Fragments arranged by size:
3678 2862 1000 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3134 5534 6744 3134
Size: 2400 1210 4032
Fragments arranged by size:
4032 2400 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7642
BfrBI ATG'CAT
Cuts at: 4556 4556
Size: 7642
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6253 136
Size: 107 122 71 631 605 4581 1525
Fragments arranged by size:
4581 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4860 7637 4860
Size: 2777 4865
BlpI GC'TnA_GC
Cuts at: 5059 5059
Size: 7642
Bme1580I G_kGCm'C
Cuts at: 1790 2997 5703 6859 1790
Size: 1207 2706 1156 2573
Fragments arranged by size:
2706 2573 1207 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 2190 5373
Size: 416 53 83 399 24 647 126 3183
Cuts at: 5373 6331 442
Size: 958 1753
Fragments arranged by size:
3183 1753 958 647 416 399 126 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 7642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2695 3074 3396 3626 4347 4379 4799
Size: 918 538 379 322 230 721 32 420
Cuts at: 4799 6302 1239
Size: 1503 2579
Fragments arranged by size:
2579 1503 918 721 538 420 379 322
230 32
Bpu10I CC'TnA_GC
Cuts at: 2922 3989 4623 2922
Size: 1067 634 5941
Fragments arranged by size:
5941 1067 634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 5767 6632 6930 3209
Size: 2558 865 298 3921
Fragments arranged by size:
3921 2558 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3200 6305 882
Size: 617 1701 3105 2219
Fragments arranged by size:
3105 2219 1701 617
BsaAI yAC'GTr
Cuts at: 493 1204 5433 493
Size: 711 4229 2702
Fragments arranged by size:
4229 2702 711
BsaBI GATnn'nnATC
Cuts at: 4821 7636 4821
Size: 2815 4827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5449 5831 6466 275
Size: 53 83 186 936 3916 382 635 1451
Fragments arranged by size:
3916 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4217 6075 6473 6747 6894 7305 4217
Size: 1858 398 274 147 411 4554
Fragments arranged by size:
4554 1858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7417
Fragments arranged by size:
7417 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2001 3546 1186
Size: 815 1545 5282
Fragments arranged by size:
5282 1545 815
BseYI C'CCAG_C
Cuts at: 6845 7341 6845
Size: 496 7146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4829 1163
Size: 573 3093 3976
Fragments arranged by size:
3976 3093 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5092 5853 6881 1540
Size: 275 186 3091 761 1028 2301
Fragments arranged by size:
3091 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3643 3932 5703 5788 6859 3643
Size: 289 1771 85 1071 4426
Fragments arranged by size:
4426 1771 1071 289 85
BsmI GAATG_Cn'
Cuts at: 3067 5153 5246 3067
Size: 2086 93 5463
Fragments arranged by size:
5463 2086 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2349 2349
Size: 7642
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2997 3643 3932 4961 5703 5788 6859
Size: 399 1207 646 289 1029 742 85 1071
Cuts at: 6859 1391
Size: 2174
Fragments arranged by size:
2174 1207 1071 1029 742 646 399 289
85
BspEI T'CCGG_A
Cuts at: 7305 7305
Size: 7642
BspHI T'CATG_A
Cuts at: 3292 4180 5531 7261 3292
Size: 888 1351 1730 3673
Fragments arranged by size:
3673 1730 1351 888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 4997 844
Size: 2215 1938 3489
Fragments arranged by size:
3489 2215 1938
BsrBI CCG'CTC
Cuts at: 1394 1823 5529 1394
Size: 429 3706 3507
Fragments arranged by size:
3706 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3781 6137 6311 65
Size: 1183 2533 2356 174 1396
Fragments arranged by size:
2533 2356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4671 6286 6473 1700
Size: 110 2861 1615 187 2869
Fragments arranged by size:
2869 2861 1615 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7642
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7642
BssSI C'ACGA_G
Cuts at: 5702 6714 5702
Size: 1012 6630
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3022 794
Size: 2228 5414
BstBI TT'CG_AA
Cuts at: 2041 2482 6446 2041
Size: 441 3964 3237
Fragments arranged by size:
3964 3237 441
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7642
BstYI r'GATC_y
Cuts at: 1972 2078 2704 2828 4303 4370 4860 4972 5339
Size: 106 626 124 1475 67 490 112 367
Cuts at: 5339 5724 5741 7193 7279 7291 7637 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1475 1452 626 490 385 367 346
124 112 106 86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2664 2664
Size: 7642
BtgI C'CryG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 5027
Fragments arranged by size:
5027 2063 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6903
Fragments arranged by size:
6903 683 56
BtsI GCAGTG_nn'
Cuts at: 5158 5952 5972 5158
Size: 794 20 6828
Fragments arranged by size:
6828 794 20
ClaI AT'CG_AT
Cuts at: 1554 2513 1554
Size: 959 6683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7607
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6649 809
Size: 507 5333 1802
Fragments arranged by size:
5333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5089 5978 7607
Size: 54 1425 50 275 18 3259 889 1629
Cuts at: 7607 8
Size: 43
Fragments arranged by size:
3259 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2760 2848 4116 4419 5572 1353
Size: 1407 88 1268 303 1153 3423
Fragments arranged by size:
3423 1407 1268 1153 303 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6229 6603 6749 387
Size: 1064 138 4640 374 146 1280
Fragments arranged by size:
4640 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3930 3930
Size: 7642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2157 2695 3074 3396 3626 4347
Size: 393 404 121 538 379 322 230 721
Cuts at: 4347 4379 4421 4638 4698 4799 5705 6302 7089
Size: 32 42 217 60 101 906 597 787
Cuts at: 7089 7357 1239
Size: 268 1524
Fragments arranged by size:
1524 906 787 721 597 538 404 393
379 322 268 230 217 121 101 60
42 32
EcoO109I rG'GnC_Cy
Cuts at: 2385 3676 2385
Size: 1291 6351
EcoRI G'AATT_C
Cuts at: 2037 2142 3871 4951 5329 2037
Size: 105 1729 1080 378 4350
Fragments arranged by size:
4350 1729 1080 378 105
EcoRV GAT'ATC
Cuts at: 2631 3193 2631
Size: 562 7080
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3593 3949 4142 4879 3593
Size: 356 193 737 6356
Fragments arranged by size:
6356 737 356 193
FspI TGC'GCA
Cuts at: 3828 6148 3828
Size: 2320 5322
FspAI rTGC'GCAy
Cuts at: 3828 3828
Size: 7642
HaeII r_GCGC'y
Cuts at: 1678 6789 1678
Size: 5111 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2666 2698 3474
Size: 32 135 32 439 32 623 32 776
Cuts at: 3474 3506 4559 4591 4639 4671 4915 4947 6283
Size: 32 1053 32 48 32 244 32 1336
Cuts at: 6283 6315 6357 6389 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1336 1053 776 623 439 244 135
48 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2226 4986 5232 669
Size: 988 281 288 2760 246 3079
Fragments arranged by size:
3079 2760 988 288 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7642
HpaI GTT'AAC
Cuts at: 2226 5232 2226
Size: 3006 4636
KpnI G_GTAC'C
Cuts at: 4967 4967
Size: 7642
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3800 6756 6940 1305
Size: 2495 2956 184 2007
Fragments arranged by size:
2956 2495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5820
Fragments arranged by size:
5820 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4185 4913 5600 5959 6118 518
Size: 648 3019 728 687 359 159 2042
Fragments arranged by size:
3019 2042 728 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2746 3322 4136 5058 5735 6883 7128 1606
Size: 1140 576 814 922 677 1148 245 2120
Fragments arranged by size:
2120 1148 1140 922 814 677 576 245
NcoI C'CATG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 5027
Fragments arranged by size:
5027 2063 552
NdeI CA'TA_TG
Cuts at: 387 3831 387
Size: 3444 4198
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NsiI A_TGCA'T
Cuts at: 4558 4558
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 3480 5000 6545 1132
Size: 97 2251 1520 1545 2229
Fragments arranged by size:
2251 2229 1545 1520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7642
PciI A'CATG_T
Cuts at: 6541 6541
Size: 7642
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7642
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2385 3676 2385
Size: 1291 6351
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7642
PsiI TTA'TAA
Cuts at: 5212 5212
Size: 7642
PstI C_TGCA'G
Cuts at: 830 2332 3027 4994 830
Size: 1502 695 1967 3478
Fragments arranged by size:
3478 1967 1502 695
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 2746 3322 4136 2746
Size: 576 814 6252
Fragments arranged by size:
6252 814 576
SacI G_AGCT'C
Cuts at: 3932 3932
Size: 7642
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SapI GCTCTTCn'nnn_
Cuts at: 4116 4419 4116
Size: 303 7339
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 5890 7602 5890
Size: 1712 5930
SfcI C'TryA_G
Cuts at: 826 1045 2328 2523 3023 3252 4990 6125 6806
Size: 219 1283 195 500 229 1738 1135 681
Cuts at: 6806 6997 826
Size: 191 1471
Fragments arranged by size:
1738 1471 1283 1135 681 500 229 219
195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7642
SmaI CCC'GGG
Cuts at: 1872 4969 1872
Size: 3097 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 5746 6647 6909 820
Size: 197 926 1281 2522 901 262 1553
Fragments arranged by size:
2522 1553 1281 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 5000 1132
Size: 3868 3774
SspI AAT'ATT
Cuts at: 5 52 5566 5
Size: 47 5514 2081
Fragments arranged by size:
5514 2081 47
StuI AGG'CCT
Cuts at: 1517 4937 1517
Size: 3420 4222
StyI C'CwwG_G
Cuts at: 513 1065 2147 2832 3128 3671 3814 3933 5081
Size: 552 1082 685 296 543 143 119 1148
Cuts at: 5081 513
Size: 3074
Fragments arranged by size:
3074 1148 1082 685 552 543 296 143
119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5669 5686 5839 6024 6469 325
Size: 1255 4089 17 153 185 445 1498
Fragments arranged by size:
4089 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4605 5888 7600
Size: 274 80 33 51 1443 2628 1283 1712
Cuts at: 7600 96
Size: 138
Fragments arranged by size:
2628 1712 1443 1283 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7602 7634 7602
Size: 32 7610
Tth111I GACn'n_nGTC
Cuts at: 1316 3772 1316
Size: 2456 5186
XbaI T'CTAG_A
Cuts at: 2299 3842 4978 2299
Size: 1543 1136 4963
Fragments arranged by size:
4963 1543 1136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2905 2969 3931 2905
Size: 64 962 6616
Fragments arranged by size:
6616 962 64
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 4967 1870
Size: 3097 4545
XmnI GAAnn'nnTTC
Cuts at: 4125 5771 4125
Size: 1646 5996
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BsiWI
BstXI BstZ17I DraIII EcoNI FseI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI