(Circular) (Six-base) MAPSORT of: pFN21AA1592.seq Check: 6024 from: 1 to: 6598 pFN21AA1592 6598 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3591 4408 278 Size: 53 83 186 936 2055 817 2468 Fragments arranged by size: 2468 2055 936 817 186 83 53 AccI GT'mk_AC Cuts at: 1937 3941 1937 Size: 2004 4594 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 4725 5098 1563 Size: 3162 373 3063 Fragments arranged by size: 3162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 3649 4661 6045 6313 1632 Size: 443 1574 1012 1384 268 1917 Fragments arranged by size: 1917 1574 1384 1012 443 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 3111 5497 6474 1203 Size: 1908 2386 977 1327 Fragments arranged by size: 2386 1908 1327 977 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 2815 5327 2815 Size: 2512 4086 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6598 AlwNI CAG_nnn'CTG Cuts at: 2440 4341 5913 2440 Size: 1901 1572 3125 Fragments arranged by size: 3125 1901 1572 ApaI G_GGCC'C Cuts at: 2035 2049 2035 Size: 14 6584 ApaLI G'TGCA_C Cuts at: 2023 2918 3778 4655 5811 2023 Size: 895 860 877 1156 2810 Fragments arranged by size: 2810 1156 895 877 860 ApoI r'AATT_y Cuts at: 1493 2492 3907 4133 4285 6394 1493 Size: 999 1415 226 152 2109 1697 Fragments arranged by size: 2109 1697 1415 999 226 152 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2183 3923 1870 Size: 73 85 155 1740 4545 Fragments arranged by size: 4545 1740 155 85 73 AvrII C'CTAG_G Cuts at: 2314 2314 Size: 6598 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3296 3329 3296 Size: 33 6565 BamHI G'GATC_C Cuts at: 3928 4295 3928 Size: 367 6231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 2468 2663 3473 3919 Size: 325 217 627 447 234 195 810 446 Cuts at: 3919 5374 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 810 627 447 446 325 234 217 195 BanII G_rGCy'C Cuts at: 1391 2035 2049 2274 2622 2649 3198 3917 1391 Size: 644 14 225 348 27 549 719 4072 Fragments arranged by size: 4072 719 644 549 348 225 27 14 BbeI G_GCGC'C Cuts at: 3477 3477 Size: 6598 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2814 2920 928 Size: 971 915 106 4606 Fragments arranged by size: 4606 971 915 106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4789 4823 1903 Size: 34 2852 34 3678 Fragments arranged by size: 3678 2852 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4490 5700 4490 Size: 1210 5388 BclI T'GATC_A Cuts at: 1548 1548 Size: 6598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2044 2119 2278 Size: 107 122 71 631 605 372 75 159 Cuts at: 2278 5209 136 Size: 2931 1525 Fragments arranged by size: 2931 1525 631 605 372 159 122 107 75 71 BglII A'GATC_T Cuts at: 6593 6593 Size: 6598 BlpI GC'TnA_GC Cuts at: 3556 4015 3556 Size: 459 6139 Bme1580I G_kGCm'C Cuts at: 1790 2027 2035 2049 2668 2922 3679 3782 4659 Size: 237 8 14 619 254 757 103 877 Cuts at: 4659 5815 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 877 757 619 254 237 103 14 8 BmgBI CAC'GTC Cuts at: 1318 3067 1318 Size: 1749 4849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4329 5287 442 Size: 416 53 83 399 24 2912 958 1753 Fragments arranged by size: 2912 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6598 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3877 3877 Size: 6598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2696 2978 3080 5258 1239 Size: 1457 282 102 2178 2579 Fragments arranged by size: 2579 2178 1457 282 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2870 3293 3320 4723 5588 5886 2870 Size: 423 27 1403 865 298 3582 Fragments arranged by size: 3582 1403 865 423 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3262 3827 5261 882 Size: 617 1763 565 1434 2219 Fragments arranged by size: 2219 1763 1434 617 565 BsaAI yAC'GTr Cuts at: 493 1204 4389 493 Size: 711 3185 2702 Fragments arranged by size: 3185 2702 711 BsaBI GATnn'nnATC Cuts at: 6592 6592 Size: 6598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3474 3588 4405 4787 Size: 53 83 186 936 1941 114 817 382 Cuts at: 4787 5422 275 Size: 635 1451 Fragments arranged by size: 1941 1451 936 817 635 382 186 114 83 53 BsaWI w'CCGG_w Cuts at: 5031 5429 5703 5850 6261 5031 Size: 398 274 147 411 5368 Fragments arranged by size: 5368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3863 3893 1178 Size: 30 165 30 2460 30 3883 Fragments arranged by size: 3883 2460 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2383 2657 2975 3089 3212 1186 Size: 922 275 274 318 114 123 4572 Fragments arranged by size: 4572 922 318 275 274 123 114 BseYI C'CCAG_C Cuts at: 2034 2706 3529 5801 6297 2034 Size: 672 823 2272 496 2335 Fragments arranged by size: 2335 2272 823 672 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2243 2860 3017 3854 1163 Size: 573 372 135 617 157 837 3907 Fragments arranged by size: 3907 837 617 573 372 157 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3399 3523 4048 4809 5837 1540 Size: 275 186 1398 124 525 761 1028 2301 Fragments arranged by size: 2301 1398 1028 761 525 275 186 124 BsiHKAI G_wGCw'C Cuts at: 2027 2447 2649 2922 3198 3351 3782 4659 4744 Size: 420 202 273 276 153 431 877 85 Cuts at: 4744 5815 2027 Size: 1071 2810 Fragments arranged by size: 2810 1071 877 431 420 276 273 202 153 85 BsmI GAATG_Cn' Cuts at: 3895 4109 4202 3895 Size: 214 93 6291 Fragments arranged by size: 6291 214 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3461 3595 3461 Size: 134 6464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2035 2049 2274 2447 2622 2649 Size: 399 237 8 14 225 173 175 27 Cuts at: 2649 2668 2922 3198 3351 3679 3782 3917 4659 Size: 19 254 276 153 328 103 135 742 Cuts at: 4659 4744 5815 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 276 254 237 225 175 173 153 135 103 85 27 19 14 8 BspEI T'CCGG_A Cuts at: 6261 6261 Size: 6598 BspHI T'CATG_A Cuts at: 2700 2724 4487 6217 2700 Size: 24 1763 1730 3081 Fragments arranged by size: 3081 1763 1730 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3953 844 Size: 3109 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3233 3555 4485 1394 Size: 429 1410 322 930 3507 Fragments arranged by size: 3507 1410 930 429 322 BsrDI GCAATG_nn' Cuts at: 65 1248 5093 5267 65 Size: 1183 3845 174 1396 Fragments arranged by size: 3845 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3394 5242 5429 1700 Size: 110 1584 1848 187 2869 Fragments arranged by size: 2869 1848 1584 187 110 BsrGI T'GTAC_A Cuts at: 96 3114 3378 96 Size: 3018 264 3316 Fragments arranged by size: 3316 3018 264 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 2633 3680 3884 4658 5670 2633 Size: 1047 204 774 1012 3561 Fragments arranged by size: 3561 1047 1012 774 204 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6598 BstBI TT'CG_AA Cuts at: 2805 5402 2805 Size: 2597 4001 BstEII G'GTnAC_C Cuts at: 1184 3023 3052 1184 Size: 1839 29 4730 Fragments arranged by size: 4730 1839 29 BstXI CCAn_nnnn'nTGG Cuts at: 3790 3790 Size: 6598 BstYI r'GATC_y Cuts at: 1972 2424 3093 3291 3928 4295 4680 4697 6149 Size: 452 669 198 637 367 385 17 1452 Cuts at: 6149 6235 6247 6593 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 669 637 452 385 367 346 198 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2433 2433 Size: 6598 BtgI C'CryG_G Cuts at: 513 1065 2205 513 Size: 552 1140 4906 Fragments arranged by size: 4906 1140 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2751 3221 487 Size: 683 56 1525 470 3864 Fragments arranged by size: 3864 1525 683 470 56 BtsI GCAGTG_nn' Cuts at: 2075 4114 4908 4928 2075 Size: 2039 794 20 3745 Fragments arranged by size: 3745 2039 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 3902 3902 Size: 6598 DraIII CAC_nnn'GTG Cuts at: 2917 2917 Size: 6598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3668 5605 809 Size: 507 2352 1937 1802 Fragments arranged by size: 2352 1937 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2988 3228 3396 Size: 54 1425 50 275 18 1158 240 168 Cuts at: 3396 3801 4045 4934 6563 8 Size: 405 244 889 1629 43 Fragments arranged by size: 1629 1425 1158 889 405 275 244 240 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3396 4045 1537 Size: 275 1584 649 4090 Fragments arranged by size: 4090 1584 649 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 3849 4528 1353 Size: 734 1762 679 3423 Fragments arranged by size: 3423 1762 734 679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5185 5559 5705 387 Size: 1064 138 3596 374 146 1280 Fragments arranged by size: 3596 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2647 3196 2647 Size: 549 6049 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2696 2978 3080 3649 4661 5258 Size: 393 443 621 282 102 569 1012 597 Cuts at: 5258 6045 6313 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1012 787 621 597 569 443 393 282 268 102 EcoNI CCTnn'n_nnAGG Cuts at: 2432 2432 Size: 6598 EcoO109I rG'GnC_Cy Cuts at: 2046 2437 3040 2046 Size: 391 603 5604 Fragments arranged by size: 5604 603 391 EcoRI G'AATT_C Cuts at: 3907 4285 3907 Size: 378 6220 EcoRV GAT'ATC Cuts at: 2698 3282 2698 Size: 584 6014 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3181 3181 Size: 6598 FspI TGC'GCA Cuts at: 2216 5104 2216 Size: 2888 3710 HaeII r_GCGC'y Cuts at: 1678 2254 3477 5745 1678 Size: 576 1223 2268 2531 Fragments arranged by size: 2531 2268 1223 576 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2416 2448 2801 Size: 32 135 32 485 32 327 32 353 Cuts at: 2801 2833 2881 2913 3826 3858 5239 5271 5313 Size: 32 48 32 913 32 1381 32 42 Cuts at: 5313 5345 1373 Size: 32 2626 Fragments arranged by size: 2626 1381 913 485 353 327 135 48 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2332 3942 4188 669 Size: 988 281 394 1610 246 3079 Fragments arranged by size: 3079 1610 988 394 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6598 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KasI G'GCGC_C Cuts at: 3473 3473 Size: 6598 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2745 2925 3069 3132 3543 5712 5896 1305 Size: 1440 180 144 63 411 2169 184 2007 Fragments arranged by size: 2169 2007 1440 411 184 180 144 63 MscI TGG'CCA Cuts at: 10 64 1489 1832 2990 10 Size: 54 1425 343 1158 3618 Fragments arranged by size: 3618 1425 1158 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2798 3788 3888 4556 4915 5074 Size: 648 1218 414 990 100 668 359 159 Cuts at: 5074 518 Size: 2042 Fragments arranged by size: 2042 1218 990 668 648 414 359 159 100 MspA1I CmG'CkG Cuts at: 1606 4014 4691 5839 6084 1606 Size: 2408 677 1148 245 2120 Fragments arranged by size: 2408 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3396 3396 Size: 6598 NarI GG'CG_CC Cuts at: 3474 3474 Size: 6598 NcoI C'CATG_G Cuts at: 513 1065 2205 513 Size: 552 1140 4906 Fragments arranged by size: 4906 1140 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NgoMIV G'CCGG_C Cuts at: 3394 3394 Size: 6598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6598 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 3115 3956 5501 1132 Size: 97 1886 841 1545 2229 Fragments arranged by size: 2229 1886 1545 841 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6598 PciI A'CATG_T Cuts at: 3111 5497 3111 Size: 2386 4212 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6598 PfoI T'CCnGG_A Cuts at: 2541 2712 2745 3096 2541 Size: 171 33 351 6043 Fragments arranged by size: 6043 351 171 33 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3040 3040 Size: 6598 PshAI GACnn'nnGTC Cuts at: 1613 3509 1613 Size: 1896 4702 PsiI TTA'TAA Cuts at: 4168 4168 Size: 6598 PspOMI G'GGCC_C Cuts at: 2031 2045 2031 Size: 14 6584 PstI C_TGCA'G Cuts at: 830 3423 3601 3950 830 Size: 2593 178 349 3478 Fragments arranged by size: 3478 2593 349 178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 SacI G_AGCT'C Cuts at: 2649 3198 2649 Size: 549 6049 SalI G'TCGA_C Cuts at: 1936 3940 1936 Size: 2004 4594 SapI GCTCTTCn'nnn_ Cuts at: 2087 2087 Size: 6598 SbfI CC_TGCA'GG Cuts at: 3423 3950 3423 Size: 527 6071 ScaI AGT'ACT Cuts at: 3359 4846 6558 3359 Size: 1487 1712 3399 Fragments arranged by size: 3399 1712 1487 SfcI C'TryA_G Cuts at: 826 1045 2688 3419 3597 3946 5081 5762 5953 Size: 219 1643 731 178 349 1135 681 191 Cuts at: 5953 826 Size: 1471 Fragments arranged by size: 1643 1471 1135 731 681 349 219 191 178 SfoI GGC'GCC Cuts at: 3475 3475 Size: 6598 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 2030 3925 1872 Size: 158 1895 4545 Fragments arranged by size: 4545 1895 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 2885 3308 3335 4702 5603 5865 Size: 197 926 942 423 27 1367 901 262 Cuts at: 5865 820 Size: 1553 Fragments arranged by size: 1553 1367 942 926 901 423 262 197 27 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6598 SphI G_CATG'C Cuts at: 1132 3956 1132 Size: 2824 3774 SspI AAT'ATT Cuts at: 5 52 2824 4522 5 Size: 47 2772 1698 2081 Fragments arranged by size: 2772 2081 1698 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6598 StyI C'CwwG_G Cuts at: 513 1065 2205 2314 3606 4037 513 Size: 552 1140 109 1292 431 3074 Fragments arranged by size: 3074 1292 1140 552 431 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2456 3499 3800 4625 4642 4795 4980 Size: 1255 876 1043 301 825 17 153 185 Cuts at: 4980 5425 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1043 876 825 445 301 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3114 3357 3378 Size: 274 80 33 51 1443 1137 243 21 Cuts at: 3378 3651 3693 4844 6556 96 Size: 273 42 1151 1712 138 Fragments arranged by size: 1712 1443 1151 1137 274 273 243 138 80 51 42 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3863 3895 6558 6590 3863 Size: 32 2663 32 3871 Fragments arranged by size: 3871 2663 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6598 XbaI T'CTAG_A Cuts at: 2353 3934 2353 Size: 1581 5017 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6598 XmaI C'CCGG_G Cuts at: 1870 2028 3923 1870 Size: 158 1895 4545 Fragments arranged by size: 4545 1895 158 XmnI GAAnn'nnTTC Cuts at: 4727 4727 Size: 6598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3589 4406 276 Size: 53 83 186 936 2055 817 2468 Fragments arranged by size: 2468 2055 936 817 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbvCI BfrBI Bpu10I BsiWI BstZ17I FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI