Restriction map for
pFN21AA1582
(Circular) (Six-base) MAPSORT of: pFN21AA1582.seq Check: 7401 from: 1 to: 9775
pFN21AA1582 9775 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2676 3553 2676
Size: 877 8898
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4796 6231 7585 278
Size: 53 83 186 936 3260 1435 1354 2468
Fragments arranged by size:
3260 2468 1435 1354 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7118 1937
Size: 5181 4594
Acc65I G'GTAC_C
Cuts at: 4093 4184 7096 4093
Size: 91 2912 6772
Fragments arranged by size:
6772 2912 91
AclI AA'CG_TT
Cuts at: 1563 5491 7902 8275 1563
Size: 3928 2411 373 3063
Fragments arranged by size:
3928 3063 2411 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2748 3501 5141 7838 9222 9490 1632
Size: 1116 753 1640 2697 1384 268 1917
Fragments arranged by size:
2697 1917 1640 1384 1116 753 268
AfeI AGC'GCT
Cuts at: 4924 4924
Size: 9775
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9578
AflIII A'CryG_T
Cuts at: 1203 4233 4701 6714 8674 9651 1203
Size: 3030 468 2013 1960 977 1327
Fragments arranged by size:
3030 2013 1960 1327 977 468
AgeI A'CCGG_T
Cuts at: 4096 5849 8606 4096
Size: 1753 2757 5265
Fragments arranged by size:
5265 2757 1753
AhdI GACnn_n'nnGTC
Cuts at: 8504 8504
Size: 9775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2882 3393 5583 5706 6956 1184
Size: 1698 511 2190 123 1250 4003
Fragments arranged by size:
4003 2190 1698 1250 511 123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 9743
AlwNI CAG_nnn'CTG
Cuts at: 3524 3887 3924 4749 5161 5632 6546 6799 7518
Size: 363 37 825 412 471 914 253 719
Cuts at: 7518 9090 3524
Size: 1572 4209
Fragments arranged by size:
4209 1572 914 825 719 471 412 363
253 37
ApaI G_GGCC'C
Cuts at: 6986 6986
Size: 9775
ApaLI G'TGCA_C
Cuts at: 5142 7832 8988 5142
Size: 2690 1156 5929
Fragments arranged by size:
5929 2690 1156
ApoI r'AATT_y
Cuts at: 1493 3386 5200 6167 7084 7310 7462 9571 1493
Size: 1893 1814 967 917 226 152 2109 1697
Fragments arranged by size:
2109 1893 1814 1697 967 917 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2292 5433 8329 160
Size: 872 1260 3141 2896 1606
Fragments arranged by size:
3141 2896 1606 1260 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9775
AvaI C'yCGr_G
Cuts at: 1870 1943 3604 4861 6502 7100 1870
Size: 73 1661 1257 1641 598 4545
Fragments arranged by size:
4545 1661 1641 1257 598 73
AvrII C'CTAG_G
Cuts at: 3401 3401
Size: 9775
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4654 4687 4654
Size: 33 9742
BamHI G'GATC_C
Cuts at: 2852 5540 7105 7472 2852
Size: 2688 1565 367 5155
Fragments arranged by size:
5155 2688 1565 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 4093 4184 6394 6467
Size: 325 217 627 282 2024 91 2210 73
Cuts at: 6467 6955 7096 8551 618
Size: 488 141 1455 1842
Fragments arranged by size:
2210 2024 1842 1455 627 488 325 282
217 141 91 73
BanII G_rGCy'C
Cuts at: 1391 5088 6986 7094 1391
Size: 3697 1898 108 4072
Fragments arranged by size:
4072 3697 1898 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2057 4796 5025 5766 928
Size: 971 158 2739 229 741 4937
Fragments arranged by size:
4937 2739 971 741 229 158
BbvCI CC'TCA_GC
Cuts at: 4189 4597 4875 4189
Size: 408 278 9089
Fragments arranged by size:
9089 408 278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3792 3826 5682 5716 6532 6566 7966
Size: 34 1855 34 1856 34 816 34 1400
Cuts at: 7966 8000 1903
Size: 34 3678
Fragments arranged by size:
3678 1856 1855 1400 816 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7667 8877 7667
Size: 1210 8565
BclI T'GATC_A
Cuts at: 1548 4833 4909 1548
Size: 3285 76 6414
Fragments arranged by size:
6414 3285 76
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3167 6480 6921
Size: 107 122 71 631 605 1495 3313 441
Cuts at: 6921 6981 8386 136
Size: 60 1405 1525
Fragments arranged by size:
3313 1525 1495 1405 631 605 441 122
107 71 60
BglII A'GATC_T
Cuts at: 3908 9770 3908
Size: 5862 3913
BlpI GC'TnA_GC
Cuts at: 6851 7058 7192 6851
Size: 207 134 9434
Fragments arranged by size:
9434 207 134
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2113 3297 5146 6399 6960 6986 7836
Size: 233 90 1184 1849 1253 561 26 850
Cuts at: 7836 8992 1790
Size: 1156 2573
Fragments arranged by size:
2573 1849 1253 1184 1156 850 561 233
90 26
BmgBI CAC'GTC
Cuts at: 1318 4702 4948 6336 1318
Size: 3384 246 1388 4757
Fragments arranged by size:
4757 3384 1388 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2635 3246 3757
Size: 416 53 83 399 24 1218 611 511
Cuts at: 3757 3976 4012 4048 5145 5316 5872 5878 5893
Size: 219 36 36 1097 171 556 6 15
Cuts at: 5893 5928 6261 6955 7506 8464 442
Size: 35 333 694 551 958 1753
Fragments arranged by size:
1753 1218 1097 958 694 611 556 551
511 416 399 333 219 171 83 53
36 36 35 24 15 6
BmtI G_CTAG'C
Cuts at: 1056 4011 1056
Size: 2955 6820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6444 6444
Size: 9775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2161 3799 5972 5991 6006 6414 6535 6952
Size: 922 1638 2173 19 15 408 121 417
Cuts at: 6952 8435 1239
Size: 1483 2579
Fragments arranged by size:
2579 2173 1638 1483 922 417 408 121
19 15
Bpu10I CC'TnA_GC
Cuts at: 4189 4597 4875 4189
Size: 408 278 9089
Fragments arranged by size:
9089 408 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2691 5319 6284 6633 7900 8765 9063 2691
Size: 2628 965 349 1267 865 298 3403
Fragments arranged by size:
3403 2628 1267 965 865 349 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5408 8438 882
Size: 617 3909 3030 2219
Fragments arranged by size:
3909 3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6110 7566 493
Size: 711 4906 1456 2702
Fragments arranged by size:
4906 2702 1456 711
BsaBI GATnn'nnATC
Cuts at: 9769 9769
Size: 9775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4334 4793 6228 7582
Size: 53 83 186 936 2801 459 1435 1354
Cuts at: 7582 7964 8599 275
Size: 382 635 1451
Fragments arranged by size:
2801 1451 1435 1354 936 635 459 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4096 5537 5849 6666 8208 8606 8880 9027 9438
Size: 1441 312 817 1542 398 274 147 411
Cuts at: 9438 4096
Size: 4433
Fragments arranged by size:
4433 1542 1441 817 411 398 312 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2805 2835 3137 3167 3689
Size: 30 165 30 1402 30 302 30 522
Cuts at: 3689 3719 5776 5806 5900 5930 1178
Size: 30 2057 30 94 30 5023
Fragments arranged by size:
5023 2057 1402 522 302 165 94 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4059 4726 4892 6287 6445 6490 6698 1186
Size: 2873 667 166 1395 158 45 208 4263
Fragments arranged by size:
4263 2873 1395 667 208 166 158 45
BseYI C'CCAG_C
Cuts at: 2262 2320 2996 3924 4131 4410 4517 4638 4963
Size: 58 676 928 207 279 107 121 325
Cuts at: 4963 5182 5316 5523 6989 8978 9474 2262
Size: 219 134 207 1466 1989 496 2563
Fragments arranged by size:
2563 1989 1466 928 676 496 325 279
219 207 207 134 121 107 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3468 3804 5318 5397 5666 6396 6695
Size: 573 1732 336 1514 79 269 730 299
Cuts at: 6695 1163
Size: 4243
Fragments arranged by size:
4243 1732 1514 730 573 336 299 269
79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7225 7986 9014 1540
Size: 275 186 5224 761 1028 2301
Fragments arranged by size:
5224 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2483 5146 5335 6521 7836 7921 8992 2483
Size: 2663 189 1186 1315 85 1071 3266
Fragments arranged by size:
3266 2663 1315 1186 1071 189 85
BsmI GAATG_Cn'
Cuts at: 3052 7286 7379 3052
Size: 4234 93 5448
Fragments arranged by size:
5448 4234 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4325 4325
Size: 9775
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2113 2483 3297 5088 5146 5335
Size: 399 233 90 370 814 1791 58 189
Cuts at: 5335 6399 6521 6960 6986 7094 7836 7921 8992
Size: 1064 122 439 26 108 742 85 1071
Cuts at: 8992 1391
Size: 2174
Fragments arranged by size:
2174 1791 1071 1064 814 742 439 399
370 233 189 122 108 90 85 58
26
BspEI T'CCGG_A
Cuts at: 5537 9438 5537
Size: 3901 5874
BspHI T'CATG_A
Cuts at: 3495 4821 6379 7664 9394 3495
Size: 1326 1558 1285 1730 3876
Fragments arranged by size:
3876 1730 1558 1326 1285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 3553 5760 7062 7130 844
Size: 1832 877 2207 1302 68 3489
Fragments arranged by size:
3489 2207 1832 1302 877 68
BsrBI CCG'CTC
Cuts at: 1394 1823 7662 1394
Size: 429 5839 3507
Fragments arranged by size:
5839 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2607 6661 8270 8444 65
Size: 1183 1359 4054 1609 174 1396
Fragments arranged by size:
4054 1609 1396 1359 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4090 4096 4558 4827 5849 6039 8419
Size: 110 2280 6 462 269 1022 190 2380
Cuts at: 8419 8606 1700
Size: 187 2869
Fragments arranged by size:
2869 2380 2280 1022 462 269 190 187
110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9775
BssHII G'CGCG_C
Cuts at: 1925 5570 1925
Size: 3645 6130
BssSI C'ACGA_G
Cuts at: 3904 4306 5075 5636 6418 7835 8847 3904
Size: 402 769 561 782 1417 1012 4832
Fragments arranged by size:
4832 1417 1012 782 769 561 402
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2837 4589 794
Size: 2043 1752 5980
Fragments arranged by size:
5980 2043 1752
BstBI TT'CG_AA
Cuts at: 6559 8579 6559
Size: 2020 7755
BstEII G'GTnAC_C
Cuts at: 1184 2765 1184
Size: 1581 8194
BstXI CCAn_nnnn'nTGG
Cuts at: 2328 5920 6581 2328
Size: 3592 661 5522
Fragments arranged by size:
5522 3592 661
BstYI r'GATC_y
Cuts at: 1972 2852 3851 3908 5540 5598 7105 7472 7857
Size: 880 999 57 1632 58 1507 367 385
Cuts at: 7857 7874 9326 9412 9424 9770 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1632 1507 1452 999 880 385 367
346 86 58 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 5358 6529 5358
Size: 1171 8604
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 5037 5706 5919 6185 6845
Size: 552 939 351 2682 669 213 266 660
Cuts at: 6845 6871 513
Size: 26 3417
Fragments arranged by size:
3417 2682 939 669 660 552 351 266
213 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9036
Fragments arranged by size:
9036 683 56
BtsI GCAGTG_nn'
Cuts at: 2194 2728 2866 2953 3375 4123 4528 4651 6247
Size: 534 138 87 422 748 405 123 1596
Cuts at: 6247 6590 6940 7291 8085 8105 2194
Size: 343 350 351 794 20 3864
Fragments arranged by size:
3864 1596 794 748 534 422 405 351
350 343 138 123 87 20
ClaI AT'CG_AT
Cuts at: 1554 4369 1554
Size: 2815 6960
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5038 5073 529
Size: 35 4474 35 5231
Fragments arranged by size:
5231 4474 35 35
DraI TTT'AAA
Cuts at: 3981 7079 3981
Size: 3098 6677
DraIII CAC_nnn'GTG
Cuts at: 5141 5141
Size: 9775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6227 8782 809
Size: 507 4911 2555 1802
Fragments arranged by size:
4911 2555 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 3667 4989
Size: 54 1425 50 275 18 318 1519 1322
Cuts at: 4989 5703 6037 6744 6895 7222 8111 9740 8
Size: 714 334 707 151 327 889 1629 43
Fragments arranged by size:
1629 1519 1425 1322 889 714 707 334
327 318 275 151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7222 1537
Size: 275 5410 4090
Fragments arranged by size:
5410 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2584 3520 4567 4883 5076 5317 7705 1353
Size: 1231 936 1047 316 193 241 2388 3423
Fragments arranged by size:
3423 2388 1231 1047 936 316 241 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8362 8736 8882 387
Size: 1064 138 6773 374 146 1280
Fragments arranged by size:
6773 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 2748 3501 3799 5141 5972 5991
Size: 393 529 587 753 298 1342 831 19
Cuts at: 5991 6006 6414 6535 6952 7838 8435 9222 9490
Size: 15 408 121 417 886 597 787 268
Cuts at: 9490 1239
Size: 1524
Fragments arranged by size:
1524 1342 886 831 787 753 597 587
529 417 408 393 298 268 121 19
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2775 2938 2775
Size: 163 9612
EcoO109I rG'GnC_Cy
Cuts at: 2906 4201 5158 5629 5869 6982 7051 2906
Size: 1295 957 471 240 1113 69 5630
Fragments arranged by size:
5630 1295 1113 957 471 240 69
EcoRI G'AATT_C
Cuts at: 5200 6167 7084 7462 5200
Size: 967 917 378 7513
Fragments arranged by size:
7513 967 917 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5390 5390
Size: 9775
FspI TGC'GCA
Cuts at: 8281 8281
Size: 9775
HaeII r_GCGC'y
Cuts at: 1678 3596 4926 5617 6800 6996 8922 1678
Size: 1918 1330 691 1183 196 1926 2531
Fragments arranged by size:
2531 1926 1918 1330 1183 691 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3689 3721 3797 3829 4172
Size: 32 135 32 2117 32 76 32 343
Cuts at: 4172 4204 5212 5244 5756 5788 6119 6151 6314
Size: 32 1008 32 512 32 331 32 163
Cuts at: 6314 6346 8416 8448 8490 8522 1373
Size: 32 2070 32 42 32 2626
Fragments arranged by size:
2626 2117 2070 1008 512 343 331 163
135 76 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3535 5833 6286 6438 7119 7365
Size: 988 281 1597 2298 453 152 681 246
Cuts at: 7365 669
Size: 3079
Fragments arranged by size:
3079 2298 1597 988 681 453 281 246
152
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9775
HpaI GTT'AAC
Cuts at: 6438 7365 6438
Size: 927 8848
KpnI G_GTAC'C
Cuts at: 4097 4188 7100 4097
Size: 91 2912 6772
Fragments arranged by size:
6772 2912 91
MfeI C'AATT_G
Cuts at: 7374 7374
Size: 9775
MluI A'CGCG_T
Cuts at: 9651 9651
Size: 9775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 3081 3872 8889 9073 1305
Size: 993 783 791 5017 184 2007
Fragments arranged by size:
5017 2007 993 791 783 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3669 6897 10
Size: 54 1425 343 1837 3228 2888
Fragments arranged by size:
3228 2888 1837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4850 5843 7733 8092 8251 518
Size: 648 3684 993 1890 359 159 2042
Fragments arranged by size:
3684 2042 1890 993 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2324 3551 4363 4530 4874 5473 5479 5620
Size: 718 1227 812 167 344 599 6 141
Cuts at: 5620 6546 6816 6847 6860 7062 7191 7868 9016
Size: 926 270 31 13 202 129 677 1148
Cuts at: 9016 9261 1606
Size: 245 2120
Fragments arranged by size:
2120 1227 1148 926 812 718 677 599
344 270 245 202 167 141 129 31
13 6
NaeI GCC'GGC
Cuts at: 4829 4829
Size: 9775
NcoI C'CATG_G
Cuts at: 513 1065 2004 5706 5919 6185 6871 513
Size: 552 939 3702 213 266 686 3417
Fragments arranged by size:
3702 3417 939 686 552 266 213
NdeI CA'TA_TG
Cuts at: 387 5445 387
Size: 5058 4717
NgoMIV G'CCGG_C
Cuts at: 4827 4827
Size: 9775
NheI G'CTAG_C
Cuts at: 1052 4007 1052
Size: 2955 6820
NotI GC'GGCC_GC
Cuts at: 7222 7222
Size: 9775
NspI r_CATG'y
Cuts at: 1132 1229 4237 5191 6718 7133 8678 1132
Size: 97 3008 954 1527 415 1545 2229
Fragments arranged by size:
3008 2229 1545 1527 954 415 97
PasI CC'CwG_GG
Cuts at: 1348 2023 5161 1348
Size: 675 3138 5962
Fragments arranged by size:
5962 3138 675
PciI A'CATG_T
Cuts at: 4233 6714 8674 4233
Size: 2481 1960 5334
Fragments arranged by size:
5334 2481 1960
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2157 4680 6546 6877 1108
Size: 1049 2523 1866 331 4006
Fragments arranged by size:
4006 2523 1866 1049 331
PfoI T'CCnGG_A
Cuts at: 2920 6175 6276 2920
Size: 3255 101 6419
Fragments arranged by size:
6419 3255 101
PmeI GTTT'AAAC
Cuts at: 7079 7079
Size: 9775
PmlI CAC'GTG
Cuts at: 6110 6110
Size: 9775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7844 7876 9357 9389 7844
Size: 32 1481 32 8230
Fragments arranged by size:
8230 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2906 4201 5869 7051 2906
Size: 1295 1668 1182 5630
Fragments arranged by size:
5630 1668 1295 1182
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9775
PsiI TTA'TAA
Cuts at: 7345 7345
Size: 9775
PspOMI G'GGCC_C
Cuts at: 6982 6982
Size: 9775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3193 3225 3193
Size: 32 9743
PstI C_TGCA'G
Cuts at: 830 4745 5478 5592 5695 5757 6201 7127 830
Size: 3915 733 114 103 62 444 926 3478
Fragments arranged by size:
3915 3478 926 733 444 114 103 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9775
PvuII CAG'CTG
Cuts at: 2324 4363 4874 5473 5479 5620 6546 6816 2324
Size: 2039 511 599 6 141 926 270 5283
Fragments arranged by size:
5283 2039 926 599 511 270 141 6
SacII CC_GC'GG
Cuts at: 6848 6848
Size: 9775
SalI G'TCGA_C
Cuts at: 1936 7117 1936
Size: 5181 4594
SanDI GG'GwC_CC
Cuts at: 4201 4201
Size: 9775
SapI GCTCTTCn'nnn_
Cuts at: 4567 5076 4567
Size: 509 9266
SbfI CC_TGCA'GG
Cuts at: 7127 7127
Size: 9775
ScaI AGT'ACT
Cuts at: 3646 6409 6965 8023 9735 3646
Size: 2763 556 1058 1712 3686
Fragments arranged by size:
3686 2763 1712 1058 556
SfcI C'TryA_G
Cuts at: 826 1045 2010 3183 4741 5474 5588 5691 5753
Size: 219 965 1173 1558 733 114 103 62
Cuts at: 5753 6197 7123 8258 8939 9130 826
Size: 444 926 1135 681 191 1471
Fragments arranged by size:
1558 1471 1173 1135 965 926 733 681
444 219 191 114 103 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9775
SgrAI Cr'CCGG_yG
Cuts at: 1700 4558 1700
Size: 2858 6917
SmaI CCC'GGG
Cuts at: 1872 3606 7102 1872
Size: 1734 3496 4545
Fragments arranged by size:
4545 3496 1734
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2706 5334 6299 6648 7879 8780
Size: 197 926 763 2628 965 349 1231 901
Cuts at: 8780 9042 820
Size: 262 1553
Fragments arranged by size:
2628 1553 1231 965 926 901 763 349
262 197
SnaBI TAC'GTA
Cuts at: 493 7566 493
Size: 7073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9775
SphI G_CATG'C
Cuts at: 1132 5191 7133 1132
Size: 4059 1942 3774
Fragments arranged by size:
4059 3774 1942
SspI AAT'ATT
Cuts at: 5 52 6205 7699 5
Size: 47 6153 1494 2081
Fragments arranged by size:
6153 2081 1494 47
StuI AGG'CCT
Cuts at: 1517 2676 3437 4814 4883 1517
Size: 1159 761 1377 69 6409
Fragments arranged by size:
6409 1377 1159 761 69
StyI C'CwwG_G
Cuts at: 513 1065 2004 2113 3289 3308 3401 3820 5706
Size: 552 939 109 1176 19 93 419 1886
Cuts at: 5706 5919 6185 6678 6693 6787 6871 7214 513
Size: 213 266 493 15 94 84 343 3074
Fragments arranged by size:
3074 1886 1176 939 552 493 419 343
266 213 109 94 93 84 19 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2599 3516 3724 4531 4988 5153 5742
Size: 1255 1019 917 208 807 457 165 589
Cuts at: 5742 5794 6430 6820 6908 7802 7819 7972 8157
Size: 52 636 390 88 894 17 153 185
Cuts at: 8157 8602 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1019 917 894 807 636 589
457 445 390 208 185 165 153 88
52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2335 3503 3644
Size: 274 80 33 51 1443 358 1168 141
Cuts at: 3644 6407 6963 8021 9733 96
Size: 2763 556 1058 1712 138
Fragments arranged by size:
2763 1712 1443 1168 1058 556 358 274
141 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2805 2837 3137 3169 5776 5808 5900 5932 9735
Size: 32 300 32 2607 32 92 32 3803
Cuts at: 9735 9767 2805
Size: 32 2813
Fragments arranged by size:
3803 2813 2607 300 92 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6337 1316
Size: 5021 4754
XbaI T'CTAG_A
Cuts at: 5207 7111 5207
Size: 1904 7871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 3677 3963 5158 2185
Size: 1492 286 1195 6802
Fragments arranged by size:
6802 1492 1195 286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9775
XmaI C'CCGG_G
Cuts at: 1870 3604 7100 1870
Size: 1734 3496 4545
Fragments arranged by size:
4545 3496 1734
XmnI GAAnn'nnTTC
Cuts at: 6776 7904 6776
Size: 1128 8647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4794 6229 7583 276
Size: 53 83 186 936 3260 1435 1354 2468
Fragments arranged by size:
3260 2468 1435 1354 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PspXI RsrII
SacI SexAI SfiI SfoI SrfI SwaI