Restriction map for
pFN21AA1575
(Circular) (Six-base) MAPSORT of: pFN21AA1575.seq Check: 945 from: 1 to: 7717
pFN21AA1575 7717 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4990 4990
Size: 7717
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3312 5527 278
Size: 53 83 186 936 1776 2215 2468
Fragments arranged by size:
2468 2215 1776 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4041 4416 4422 5060 1937
Size: 2104 375 6 638 4594
Fragments arranged by size:
4594 2104 638 375 6
Acc65I G'GTAC_C
Cuts at: 4661 5038 4661
Size: 377 7340
AclI AA'CG_TT
Cuts at: 1563 2034 3672 5844 6217 1563
Size: 471 1638 2172 373 3063
Fragments arranged by size:
3063 2172 1638 471 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2422 3093 5780 7164 7432 1632
Size: 790 671 2687 1384 268 1917
Fragments arranged by size:
2687 1917 1384 790 671 268
AfeI AGC'GCT
Cuts at: 2499 2732 4507 2499
Size: 233 1775 5709
Fragments arranged by size:
5709 1775 233
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7520
AflIII A'CryG_T
Cuts at: 1203 2652 4464 6616 7593 1203
Size: 1449 1812 2152 977 1327
Fragments arranged by size:
2152 1812 1449 1327 977
AgeI A'CCGG_T
Cuts at: 6548 6548
Size: 7717
AhdI GACnn_n'nnGTC
Cuts at: 6446 6446
Size: 7717
AleI CACnn'nnGTG
Cuts at: 1166 4463 1166
Size: 3297 4420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7717
AlwNI CAG_nnn'CTG
Cuts at: 2701 3992 5460 7032 2701
Size: 1291 1468 1572 3386
Fragments arranged by size:
3386 1572 1468 1291
ApaLI G'TGCA_C
Cuts at: 5774 6930 5774
Size: 1156 6561
ApoI r'AATT_y
Cuts at: 1493 5026 5252 5404 7513 1493
Size: 3533 226 152 2109 1697
Fragments arranged by size:
3533 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6271 160
Size: 872 5239 1606
Fragments arranged by size:
5239 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
AvaI C'yCGr_G
Cuts at: 1870 1943 2257 2390 3027 3224 3539 5042 1870
Size: 73 314 133 637 197 315 1503 4545
Fragments arranged by size:
4545 1503 637 315 314 197 133 73
AvrII C'CTAG_G
Cuts at: 4678 4678
Size: 7717
BamHI G'GATC_C
Cuts at: 4762 5047 5414 4762
Size: 285 367 7065
Fragments arranged by size:
7065 367 285
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2597 3209 3535 3650 3743
Size: 325 217 627 810 612 326 115 93
Cuts at: 3743 3851 4086 4661 5038 6493 618
Size: 108 235 575 377 1455 1842
Fragments arranged by size:
1842 1455 810 627 612 575 377 326
325 235 217 115 108 93
BanII G_rGCy'C
Cuts at: 1391 2297 2537 3764 4264 4515 5009 5036 1391
Size: 906 240 1227 500 251 494 27 4072
Fragments arranged by size:
4072 1227 906 500 494 251 240 27
BbeI G_GCGC'C
Cuts at: 3855 3855
Size: 7717
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 3245 3327 4394 4621 928
Size: 971 192 1154 82 1067 227 4024
Fragments arranged by size:
4024 1154 1067 971 227 192 82
BbvCI CC'TCA_GC
Cuts at: 2673 2673
Size: 7717
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4715 4749 4940 4974 5908
Size: 34 50 34 2694 34 191 34 934
Cuts at: 5908 5942 1903
Size: 34 3678
Fragments arranged by size:
3678 2694 934 191 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3192 5609 6819 3192
Size: 2417 1210 4090
Fragments arranged by size:
4090 2417 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7717
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4117 6328 136
Size: 107 122 71 631 605 2445 2211 1525
Fragments arranged by size:
2445 2211 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3497 7712 3497
Size: 4215 3502
BlpI GC'TnA_GC
Cuts at: 4509 4976 5134 4509
Size: 467 158 7092
Fragments arranged by size:
7092 467 158
Bme1580I G_kGCm'C
Cuts at: 1790 3214 3225 3540 4089 4792 5778 6934 1790
Size: 1424 11 315 549 703 986 1156 2573
Fragments arranged by size:
2573 1424 1156 986 703 549 315 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7717
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 3613 4046
Size: 416 53 83 399 24 883 1313 433
Cuts at: 4046 5448 6406 442
Size: 1402 958 1753
Fragments arranged by size:
1753 1402 1313 958 883 433 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2694 3456 4727 6377 1239
Size: 1455 762 1271 1650 2579
Fragments arranged by size:
2579 1650 1455 1271 762
Bpu10I CC'TnA_GC
Cuts at: 2673 2673
Size: 7717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5842 6707 7005 5842
Size: 865 298 6554
Fragments arranged by size:
6554 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6380 882
Size: 617 4881 2219
Fragments arranged by size:
4881 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4420 4921 5508 493
Size: 711 3216 501 587 2702
Fragments arranged by size:
3216 2702 711 587 501
BsaBI GATnn'nnATC
Cuts at: 7711 7711
Size: 7717
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2880 3309 3852 5524
Size: 53 83 186 936 1347 429 543 1672
Cuts at: 5524 5906 6541 275
Size: 382 635 1451
Fragments arranged by size:
1672 1451 1347 936 635 543 429 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3193 3437 4284 6150 6548 6822 6969 7380 3193
Size: 244 847 1866 398 274 147 411 3530
Fragments arranged by size:
3530 1866 847 411 398 274 244 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3451 3481 1178
Size: 30 165 30 2048 30 5414
Fragments arranged by size:
5414 2048 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3267 3459 4238 4252 4589 1186
Size: 2081 192 779 14 337 4314
Fragments arranged by size:
4314 2081 779 337 192 14
BseYI C'CCAG_C
Cuts at: 2500 2694 2824 3045 3213 3703 3985 4475 6920
Size: 194 130 221 168 490 282 490 2445
Cuts at: 6920 7416 2500
Size: 496 2801
Fragments arranged by size:
2801 2445 496 490 490 282 221 194
168 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2701 3714 1163
Size: 573 965 1013 5166
Fragments arranged by size:
5166 1013 965 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3020 4132 5167 5928 6956 1540
Size: 275 186 1019 1112 1035 761 1028 2301
Fragments arranged by size:
2301 1112 1035 1028 1019 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2183 3435 3764 5778 5863 6934 2183
Size: 1252 329 2014 85 1071 2966
Fragments arranged by size:
2966 2014 1252 1071 329 85
BsmI GAATG_Cn'
Cuts at: 3938 5228 5321 3938
Size: 1290 93 6334
Fragments arranged by size:
6334 1290 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2419 2419
Size: 7717
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2297 2537 3214 3225 3435 3540
Size: 399 393 114 240 677 11 210 105
Cuts at: 3540 3764 4089 4264 4515 4792 5009 5036 5778
Size: 224 325 175 251 277 217 27 742
Cuts at: 5778 5863 6934 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 677 399 393 325 277
251 240 224 217 210 175 114 105
85 27 11
BspEI T'CCGG_A
Cuts at: 3193 3437 4284 7380 3193
Size: 244 847 3096 3530
Fragments arranged by size:
3530 3096 847 244
BspHI T'CATG_A
Cuts at: 5606 7336 5606
Size: 1730 5987
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2751 4649 4990 5072 844
Size: 1907 1898 341 82 3489
Fragments arranged by size:
3489 1907 1898 341 82
BsrBI CCG'CTC
Cuts at: 1394 1823 5604 1394
Size: 429 3781 3507
Fragments arranged by size:
3781 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2845 3999 4994 6212 6386 65
Size: 1183 1597 1154 995 1218 174 1396
Fragments arranged by size:
1597 1396 1218 1183 1154 995 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4127 4534 6361 6548 1700
Size: 110 2317 407 1827 187 2869
Fragments arranged by size:
2869 2317 1827 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 2655 96
Size: 2559 5158
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7717
BssSI C'ACGA_G
Cuts at: 5777 6789 5777
Size: 1012 6705
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7717
BstBI TT'CG_AA
Cuts at: 6521 6521
Size: 7717
BstEII G'GTnAC_C
Cuts at: 1184 2965 4925 1184
Size: 1781 1960 3976
Fragments arranged by size:
3976 1960 1781
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 3400
Size: 7717
BstYI r'GATC_y
Cuts at: 1972 3093 3497 4241 4762 5047 5414 5799 5816
Size: 1121 404 744 521 285 367 385 17
Cuts at: 5816 7268 7354 7366 7712 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1121 744 521 404 385 367
346 285 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3993 3993
Size: 7717
BtgI C'CryG_G
Cuts at: 513 1065 2867 3156 3393 513
Size: 552 1802 289 237 4837
Fragments arranged by size:
4837 1802 552 289 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4546 4740 487
Size: 683 56 3320 194 3464
Fragments arranged by size:
3464 3320 683 194 56
BtsI GCAGTG_nn'
Cuts at: 3513 4792 5233 6027 6047 3513
Size: 1279 441 794 20 5183
Fragments arranged by size:
5183 1279 794 441 20
ClaI AT'CG_AT
Cuts at: 1554 2196 1554
Size: 642 7075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3312 3347 529
Size: 35 2748 35 4899
Fragments arranged by size:
4899 2748 35 35
DraI TTT'AAA
Cuts at: 5021 5021
Size: 7717
DraIII CAC_nnn'GTG
Cuts at: 2167 4799 2167
Size: 2632 5085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4412 6724 809
Size: 507 3096 2312 1802
Fragments arranged by size:
3096 2312 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3017 3159 3810
Size: 54 1425 50 275 18 1187 142 651
Cuts at: 3810 4129 4498 5164 6053 7682 8
Size: 319 369 666 889 1629 43
Fragments arranged by size:
1629 1425 1187 889 666 651 369 319
275 142 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3017 4129 5164 1537
Size: 275 1205 1112 1035 4090
Fragments arranged by size:
4090 1205 1112 1035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 3552 5647 1353
Size: 786 1413 2095 3423
Fragments arranged by size:
3423 2095 1413 786
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6304 6678 6824 387
Size: 1064 138 4715 374 146 1280
Fragments arranged by size:
4715 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3762 3762
Size: 7717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2422 2694 3093 3456 4727 5780 6377
Size: 393 790 272 399 363 1271 1053 597
Cuts at: 6377 7164 7432 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1271 1053 790 787 597 399 393
363 272 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3964 4571 3964
Size: 607 7110
EcoO109I rG'GnC_Cy
Cuts at: 2254 3945 4034 4273 2254
Size: 1691 89 239 5698
Fragments arranged by size:
5698 1691 239 89
EcoRI G'AATT_C
Cuts at: 5026 5404 5026
Size: 378 7339
FspI TGC'GCA
Cuts at: 2851 6223 2851
Size: 3372 4345
HaeII r_GCGC'y
Cuts at: 1678 2501 2734 3855 4509 6864 1678
Size: 823 233 1121 654 2355 2531
Fragments arranged by size:
2531 2355 1121 823 654 233
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3050 3082 3254 3286 3489
Size: 32 135 32 1478 32 172 32 203
Cuts at: 3489 3521 4226 4258 4832 4864 6358 6390 6432
Size: 32 705 32 574 32 1494 32 42
Cuts at: 6432 6464 1373
Size: 32 2626
Fragments arranged by size:
2626 1494 1478 705 574 203 172 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5061 5307 669
Size: 988 281 3123 246 3079
Fragments arranged by size:
3123 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4409 748
Size: 3661 4056
HpaI GTT'AAC
Cuts at: 5307 5307
Size: 7717
KasI G'GCGC_C
Cuts at: 3851 3851
Size: 7717
KpnI G_GTAC'C
Cuts at: 4665 5042 4665
Size: 377 7340
MfeI C'AATT_G
Cuts at: 5316 5316
Size: 7717
MluI A'CGCG_T
Cuts at: 7593 7593
Size: 7717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3264 6831 7015 1305
Size: 1959 3567 184 2007
Fragments arranged by size:
3567 2007 1959 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4500 10
Size: 54 1425 343 2668 3227
Fragments arranged by size:
3227 2668 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4335 4463 5675 6034 6193 518
Size: 648 3169 128 1212 359 159 2042
Fragments arranged by size:
3169 2042 1212 648 359 159 128
MspA1I CmG'CkG
Cuts at: 1606 2043 2701 3158 3245 3622 4913 5133 5810
Size: 437 658 457 87 377 1291 220 677
Cuts at: 5810 6958 7203 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1291 1148 677 658 457 437 377
245 220 87
NaeI GCC'GGC
Cuts at: 4536 4536
Size: 7717
NarI GG'CG_CC
Cuts at: 3852 3852
Size: 7717
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7717
NgoMIV G'CCGG_C
Cuts at: 4534 4534
Size: 7717
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7717
NotI GC'GGCC_GC
Cuts at: 5164 5164
Size: 7717
NspI r_CATG'y
Cuts at: 1132 1229 2071 2656 3233 3580 3797 5075 6620
Size: 97 842 585 577 347 217 1278 1545
Cuts at: 6620 1132
Size: 2229
Fragments arranged by size:
2229 1545 1278 842 585 577 347 217
97
PasI CC'CwG_GG
Cuts at: 1348 2574 2888 2943 4162 1348
Size: 1226 314 55 1219 4903
Fragments arranged by size:
4903 1226 1219 314 55
PciI A'CATG_T
Cuts at: 2652 6616 2652
Size: 3964 3753
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2167 3479 4103 1108
Size: 1059 1312 624 4722
Fragments arranged by size:
4722 1312 1059 624
PfoI T'CCnGG_A
Cuts at: 2238 3472 4704 2238
Size: 1234 1232 5251
Fragments arranged by size:
5251 1234 1232
PmeI GTTT'AAAC
Cuts at: 5021 5021
Size: 7717
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2357 2389 5786 5818 7299 7331 2357
Size: 32 3397 32 1481 32 2743
Fragments arranged by size:
3397 2743 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 3945 4034 4273 2254
Size: 1691 89 239 5698
Fragments arranged by size:
5698 1691 239 89
PshAI GACnn'nnGTC
Cuts at: 1613 4394 1613
Size: 2781 4936
PsiI TTA'TAA
Cuts at: 5287 5287
Size: 7717
PstI C_TGCA'G
Cuts at: 830 3058 4545 5069 830
Size: 2228 1487 524 3478
Fragments arranged by size:
3478 2228 1487 524
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7717
PvuII CAG'CTG
Cuts at: 2043 2043
Size: 7717
SacI G_AGCT'C
Cuts at: 3764 3764
Size: 7717
SacII CC_GC'GG
Cuts at: 3159 3159
Size: 7717
SalI G'TCGA_C
Cuts at: 1936 5059 1936
Size: 3123 4594
SanDI GG'GwC_CC
Cuts at: 2254 2254
Size: 7717
SbfI CC_TGCA'GG
Cuts at: 5069 5069
Size: 7717
ScaI AGT'ACT
Cuts at: 5965 7677 5965
Size: 1712 6005
SfcI C'TryA_G
Cuts at: 826 1045 2481 2683 3054 3493 3765 4541 5065
Size: 219 1436 202 371 439 272 776 524
Cuts at: 5065 6200 6881 7072 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1436 1135 776 681 524 439 371
272 219 202 191
SfoI GGC'GCC
Cuts at: 3853 3853
Size: 7717
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7717
SmaI CCC'GGG
Cuts at: 1872 2259 3226 3541 5044 1872
Size: 387 967 315 1503 4545
Fragments arranged by size:
4545 1503 967 387 315
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5821 6722 6984 820
Size: 197 926 3878 901 262 1553
Fragments arranged by size:
3878 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4420 5508 493
Size: 3927 1088 2702
Fragments arranged by size:
3927 2702 1088
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7717
SphI G_CATG'C
Cuts at: 1132 3233 5075 1132
Size: 2101 1842 3774
Fragments arranged by size:
3774 2101 1842
SrfI GCCC'GGGC
Cuts at: 3226 3541 3226
Size: 315 7402
SspI AAT'ATT
Cuts at: 5 52 5641 5
Size: 47 5589 2081
Fragments arranged by size:
5589 2081 47
StuI AGG'CCT
Cuts at: 1517 2515 2663 1517
Size: 998 148 6571
Fragments arranged by size:
6571 998 148
StyI C'CwwG_G
Cuts at: 513 1065 2338 2784 2958 3186 4678 5156 513
Size: 552 1273 446 174 228 1492 478 3074
Fragments arranged by size:
3074 1492 1273 552 478 446 228 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2753 2809 3059 3520 4065 4913 4944
Size: 1255 1173 56 250 461 545 848 31
Cuts at: 4944 5744 5761 5914 6099 6544 325
Size: 800 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1173 848 800 545 461 445
250 185 153 56 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 5963 7675
Size: 274 80 33 51 1443 678 3308 1712
Cuts at: 7675 96
Size: 138
Fragments arranged by size:
3308 1712 1443 678 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3015 3047 4117 4149 7677 7709 3015
Size: 32 1070 32 3528 32 3023
Fragments arranged by size:
3528 3023 1070 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7717
XbaI T'CTAG_A
Cuts at: 5053 5053
Size: 7717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2195 2195
Size: 7717
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7717
XmaI C'CCGG_G
Cuts at: 1870 2257 3224 3539 5042 1870
Size: 387 967 315 1503 4545
Fragments arranged by size:
4545 1503 967 387 315
XmnI GAAnn'nnTTC
Cuts at: 5846 5846
Size: 7717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3310 5525 276
Size: 53 83 186 936 1776 2215 2468
Fragments arranged by size:
2468 2215 1776 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SapI SexAI SfiI SwaI