Restriction map for
pFN21AA1565
(Circular) (Six-base) MAPSORT of: pFN21AA1565.seq Check: 8699 from: 1 to: 8530
pFN21AA1565 8530 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4155 4155
Size: 8530
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6340 278
Size: 53 83 186 936 4804 2468
Fragments arranged by size:
4804 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5873 1937
Size: 3936 4594
Acc65I G'GTAC_C
Cuts at: 5549 5851 5549
Size: 302 8228
AclI AA'CG_TT
Cuts at: 1563 4798 6657 7030 1563
Size: 3235 1859 373 3063
Fragments arranged by size:
3235 3063 1859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2150 2388 3002 3500 3810 4025 4223 4376
Size: 518 238 614 498 310 215 198 153
Cuts at: 4376 4872 5276 6593 7977 8245 1632
Size: 496 404 1317 1384 268 1917
Fragments arranged by size:
1917 1384 1317 614 518 498 496 404
310 268 238 215 198 153
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8333
AflIII A'CryG_T
Cuts at: 1203 2118 7429 8406 1203
Size: 915 5311 977 1327
Fragments arranged by size:
5311 1327 977 915
AgeI A'CCGG_T
Cuts at: 7361 7361
Size: 8530
AhdI GACnn_n'nnGTC
Cuts at: 7259 7259
Size: 8530
AleI CACnn'nnGTG
Cuts at: 1166 4823 1166
Size: 3657 4873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5097 5795 1184
Size: 3913 698 3919
Fragments arranged by size:
3919 3913 698
AlwNI CAG_nnn'CTG
Cuts at: 3337 5413 5810 6273 7845 3337
Size: 2076 397 463 1572 4022
Fragments arranged by size:
4022 2076 1572 463 397
ApaI G_GGCC'C
Cuts at: 5320 5320
Size: 8530
ApaLI G'TGCA_C
Cuts at: 3690 6587 7743 3690
Size: 2897 1156 4477
Fragments arranged by size:
4477 2897 1156
ApoI r'AATT_y
Cuts at: 1493 3523 4432 4582 5170 5650 5839 6065 6217
Size: 2030 909 150 588 480 189 226 152
Cuts at: 6217 8326 1493
Size: 2109 1697
Fragments arranged by size:
2109 2030 1697 909 588 480 226 189
152 150
AseI AT'TA_AT
Cuts at: 160 1032 2216 7084 160
Size: 872 1184 4868 1606
Fragments arranged by size:
4868 1606 1184 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
AvaI C'yCGr_G
Cuts at: 1870 1943 2336 2675 5481 5855 1870
Size: 73 393 339 2806 374 4545
Fragments arranged by size:
4545 2806 393 374 339 73
BamHI G'GATC_C
Cuts at: 5860 6227 5860
Size: 367 8163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2802 5549 5851 7306 618
Size: 325 217 627 1015 2747 302 1455 1842
Fragments arranged by size:
2747 1842 1455 1015 627 325 302 217
BanII G_rGCy'C
Cuts at: 1391 3771 3783 3891 4185 4368 4848 5320 5349
Size: 2380 12 108 294 183 480 472 29
Cuts at: 5349 5849 1391
Size: 500 4072
Fragments arranged by size:
4072 2380 500 480 472 294 183 108
29 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2453 2878 4821 5184 928
Size: 971 554 425 1943 363 4274
Fragments arranged by size:
4274 1943 971 554 425 363
BbvCI CC'TCA_GC
Cuts at: 4160 4160
Size: 8530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6721 6755 1903
Size: 34 4784 34 3678
Fragments arranged by size:
4784 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6422 7632 6422
Size: 1210 7320
BclI T'GATC_A
Cuts at: 1548 3220 5599 1548
Size: 1672 2379 4479
Fragments arranged by size:
4479 2379 1672
BfrBI ATG'CAT
Cuts at: 2918 2918
Size: 8530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7141 136
Size: 107 122 71 631 605 5469 1525
Fragments arranged by size:
5469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3169 8525 3169
Size: 5356 3174
BlpI GC'TnA_GC
Cuts at: 2286 5947 2286
Size: 3661 4869
Bme1580I G_kGCm'C
Cuts at: 1790 2078 3694 5320 6591 7747 1790
Size: 288 1616 1626 1271 1156 2573
Fragments arranged by size:
2573 1626 1616 1271 1156 288
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2138 6261 7219
Size: 416 53 83 399 24 721 4123 958
Cuts at: 7219 442
Size: 1753
Fragments arranged by size:
4123 1753 958 721 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8530
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2690
Size: 8530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3650 3905 4856 4931 5423 7190 1239
Size: 2411 255 951 75 492 1767 2579
Fragments arranged by size:
2579 2411 1767 951 492 255 75
Bpu10I CC'TnA_GC
Cuts at: 3707 4160 3707
Size: 453 8077
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 3176 4046 4148 4169 4191 4361 6655 7520
Size: 1149 870 102 21 22 170 2294 865
Cuts at: 7520 7818 2027
Size: 298 2739
Fragments arranged by size:
2739 2294 1149 870 865 298 170 102
22 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2632 7193 882
Size: 617 1133 4561 2219
Fragments arranged by size:
4561 2219 1133 617
BsaAI yAC'GTr
Cuts at: 493 1204 6321 493
Size: 711 5117 2702
Fragments arranged by size:
5117 2702 711
BsaBI GATnn'nnATC
Cuts at: 4408 8524 4408
Size: 4116 4414
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5826 6337 6719 7354
Size: 53 83 186 936 4293 511 382 635
Cuts at: 7354 275
Size: 1451
Fragments arranged by size:
4293 1451 936 635 511 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2613 6963 7361 7635 7782 8193 2613
Size: 4350 398 274 147 411 2950
Fragments arranged by size:
4350 2950 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8305
Fragments arranged by size:
8305 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2372 2477 4112 4115 4145 4364 1186
Size: 961 225 105 1635 3 30 219 5352
Fragments arranged by size:
5352 1635 961 225 219 105 30 3
BseYI C'CCAG_C
Cuts at: 2419 5313 7733 8229 2419
Size: 2894 2420 496 2720
Fragments arranged by size:
2894 2720 2420 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3655 4829 4913 5207 5791 1163
Size: 573 1919 1174 84 294 584 3902
Fragments arranged by size:
3902 1919 1174 584 573 294 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5980 6741 7769 1540
Size: 275 186 3979 761 1028 2301
Fragments arranged by size:
3979 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2442 3694 3708 3891 4062 4185 4368 4848 6591
Size: 1252 14 183 171 123 183 480 1743
Cuts at: 6591 6676 7747 2442
Size: 85 1071 3225
Fragments arranged by size:
3225 1743 1252 1071 480 183 183 171
123 85 14
BsmI GAATG_Cn'
Cuts at: 2518 3072 3800 5302 6041 6134 2518
Size: 554 728 1502 739 93 4914
Fragments arranged by size:
4914 1502 739 728 554 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2442 3694 3708 3771 3783 3891
Size: 399 288 364 1252 14 63 12 108
Cuts at: 3891 4062 4185 4368 4848 5320 5349 5849 6591
Size: 171 123 183 480 472 29 500 742
Cuts at: 6591 6676 7747 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1252 1071 742 500 480 472 399
364 288 183 171 123 108 85 63
29 14 12
BspEI T'CCGG_A
Cuts at: 8193 8193
Size: 8530
BspHI T'CATG_A
Cuts at: 4075 6419 8149 4075
Size: 2344 1730 4456
Fragments arranged by size:
4456 2344 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4155 5208 5885 844
Size: 3311 1053 677 3489
Fragments arranged by size:
3489 3311 1053 677
BsrBI CCG'CTC
Cuts at: 1394 1823 3241 3628 4222 6417 1394
Size: 429 1418 387 594 2195 3507
Fragments arranged by size:
3507 2195 1418 594 429 387
BsrDI GCAATG_nn'
Cuts at: 65 1248 2865 7025 7199 65
Size: 1183 1617 4160 174 1396
Fragments arranged by size:
4160 1617 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2423 7174 7361 1700
Size: 110 613 4751 187 2869
Fragments arranged by size:
4751 2869 613 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8530
BssSI C'ACGA_G
Cuts at: 6590 7602 6590
Size: 1012 7518
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3337 794
Size: 2543 5987
BstBI TT'CG_AA
Cuts at: 7334 7334
Size: 8530
BstEII G'GTnAC_C
Cuts at: 1184 2603 3058 3554 1184
Size: 1419 455 496 6160
Fragments arranged by size:
6160 1419 496 455
BstYI r'GATC_y
Cuts at: 1972 2528 2976 3169 3333 4511 5860 6227 6612
Size: 556 448 193 164 1178 1349 367 385
Cuts at: 6612 6629 8081 8167 8179 8525 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1349 1178 556 448 385 367
346 193 164 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3260 5811 3260
Size: 2551 5979
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 3933 5445 513
Size: 552 939 348 1581 1512 3598
Fragments arranged by size:
3598 1581 1512 939 552 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7791
Fragments arranged by size:
7791 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 2464 3577 3638 4062 5818 6046 6840 6860
Size: 24 1113 61 424 1756 228 794 20
Cuts at: 6860 2440
Size: 4110
Fragments arranged by size:
4110 1756 1113 794 424 228 61 24
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4305 4340 529
Size: 35 3741 35 4719
Fragments arranged by size:
4719 3741 35 35
DraI TTT'AAA
Cuts at: 2206 2746 3435 4464 4587 5259 5834 2206
Size: 540 689 1029 123 672 575 4902
Fragments arranged by size:
4902 1029 689 672 575 540 123
DraIII CAC_nnn'GTG
Cuts at: 2525 2525
Size: 8530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7537 809
Size: 507 6221 1802
Fragments arranged by size:
6221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3090 3231 5977
Size: 54 1425 50 275 18 1260 141 2746
Cuts at: 5977 6866 8495 8
Size: 889 1629 43
Fragments arranged by size:
2746 1629 1425 1260 889 275 141 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5977 1537
Size: 275 4165 4090
Fragments arranged by size:
4165 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 2946 3270 3393 3588 4254 6460 1353
Size: 1197 396 324 123 195 666 2206 3423
Fragments arranged by size:
3423 2206 1197 666 396 324 195 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3259 4856 7117 7491 7637 387
Size: 1064 138 1670 1597 2261 374 146 1280
Fragments arranged by size:
2261 1670 1597 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3889 4183 4366 4846 3889
Size: 294 183 480 7573
Fragments arranged by size:
7573 480 294 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2388 3002 3500 3650 3810 3905
Size: 393 518 238 614 498 150 160 95
Cuts at: 3905 4025 4223 4376 4856 4872 4931 5276 5423
Size: 120 198 153 480 16 59 345 147
Cuts at: 5423 6593 7190 7977 8245 1239
Size: 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 787 614 597 518 498 480
393 345 268 238 198 160 153 150
147 120 95 59 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2090 3341 2090
Size: 1251 7279
EcoO109I rG'GnC_Cy
Cuts at: 2082 2328 5317 2082
Size: 246 2989 5295
Fragments arranged by size:
5295 2989 246
EcoRI G'AATT_C
Cuts at: 5650 5839 6217 5650
Size: 189 378 7963
Fragments arranged by size:
7963 378 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4142 4142
Size: 8530
FspI TGC'GCA
Cuts at: 7036 7036
Size: 8530
HaeII r_GCGC'y
Cuts at: 1678 4251 7677 1678
Size: 2573 3426 2531
Fragments arranged by size:
3426 2573 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3318 3350 3873 3905 4189
Size: 32 135 32 1746 32 523 32 284
Cuts at: 4189 4221 5317 5349 7171 7203 7245 7277 1373
Size: 32 1096 32 1822 32 42 32 2626
Fragments arranged by size:
2626 1822 1746 1096 523 284 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5874 6120 669
Size: 988 281 3936 246 3079
Fragments arranged by size:
3936 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4124 4213 4418 748
Size: 3376 89 205 4860
Fragments arranged by size:
4860 3376 205 89
HpaI GTT'AAC
Cuts at: 6120 6120
Size: 8530
KpnI G_GTAC'C
Cuts at: 5553 5855 5553
Size: 302 8228
MfeI C'AATT_G
Cuts at: 4625 5498 6129 4625
Size: 873 631 7026
Fragments arranged by size:
7026 873 631
MluI A'CGCG_T
Cuts at: 8406 8406
Size: 8530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 5364 5731 7644 7828 1305
Size: 1797 2262 367 1913 184 2007
Fragments arranged by size:
2262 2007 1913 1797 367 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3092 10
Size: 54 1425 343 1260 5448
Fragments arranged by size:
5448 1425 1260 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4823 5121 6488 6847 7006 518
Size: 648 3657 298 1367 359 159 2042
Fragments arranged by size:
3657 2042 1367 648 359 298 159
MspA1I CmG'CkG
Cuts at: 1606 3756 3922 3942 4312 4927 5143 5413 5946
Size: 2150 166 20 370 615 216 270 533
Cuts at: 5946 6623 7771 8016 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2150 2120 1148 677 615 533 370 270
245 216 166 20
NcoI C'CATG_G
Cuts at: 513 1065 2004 5445 513
Size: 552 939 3441 3598
Fragments arranged by size:
3598 3441 939 552
NdeI CA'TA_TG
Cuts at: 387 3869 387
Size: 3482 5048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8530
NotI GC'GGCC_GC
Cuts at: 5977 5977
Size: 8530
NsiI A_TGCA'T
Cuts at: 2920 2920
Size: 8530
NspI r_CATG'y
Cuts at: 1132 1229 2122 5888 7433 1132
Size: 97 893 3766 1545 2229
Fragments arranged by size:
3766 2229 1545 893 97
PasI CC'CwG_GG
Cuts at: 1348 2086 1348
Size: 738 7792
PciI A'CATG_T
Cuts at: 2118 7429 2118
Size: 5311 3219
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4627 5446 1108
Size: 3519 819 4192
Fragments arranged by size:
4192 3519 819
PfoI T'CCnGG_A
Cuts at: 3411 5210 3411
Size: 1799 6731
PmeI GTTT'AAAC
Cuts at: 5834 5834
Size: 8530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3765 3797 6599 6631 8112 8144 3765
Size: 32 2802 32 1481 32 4151
Fragments arranged by size:
4151 2802 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2082 2328 2082
Size: 246 8284
PshAI GACnn'nnGTC
Cuts at: 1613 2326 1613
Size: 713 7817
PsiI TTA'TAA
Cuts at: 6100 6100
Size: 8530
PspOMI G'GGCC_C
Cuts at: 5316 5316
Size: 8530
PspXI vC'TCGA_Gb
Cuts at: 2675 2675
Size: 8530
PstI C_TGCA'G
Cuts at: 830 2154 2435 3342 4152 4564 5882 830
Size: 1324 281 907 810 412 1318 3478
Fragments arranged by size:
3478 1324 1318 907 810 412 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8530
PvuII CAG'CTG
Cuts at: 3922 3942 4312 4927 5143 5413 3922
Size: 20 370 615 216 270 7039
Fragments arranged by size:
7039 615 370 270 216 20
SacI G_AGCT'C
Cuts at: 3891 4185 4368 4848 3891
Size: 294 183 480 7573
Fragments arranged by size:
7573 480 294 183
SalI G'TCGA_C
Cuts at: 1936 5872 1936
Size: 3936 4594
SanDI GG'GwC_CC
Cuts at: 2082 2082
Size: 8530
SbfI CC_TGCA'GG
Cuts at: 4152 5882 4152
Size: 1730 6800
ScaI AGT'ACT
Cuts at: 6778 8490 6778
Size: 1712 6818
SfcI C'TryA_G
Cuts at: 826 1045 2150 2431 3338 4148 4560 5878 7013
Size: 219 1105 281 907 810 412 1318 1135
Cuts at: 7013 7694 7885 826
Size: 681 191 1471
Fragments arranged by size:
1471 1318 1135 1105 907 810 681 412
281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8530
SmaI CCC'GGG
Cuts at: 1872 5483 5857 1872
Size: 3611 374 4545
Fragments arranged by size:
4545 3611 374
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2675 3191 4061 4127 4184
Size: 197 926 99 633 516 870 66 57
Cuts at: 4184 4206 4340 6634 7535 7797 820
Size: 22 134 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 870 633 516 262
197 134 99 66 57 22
SnaBI TAC'GTA
Cuts at: 493 6321 493
Size: 5828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8530
SphI G_CATG'C
Cuts at: 1132 5888 1132
Size: 4756 3774
SspI AAT'ATT
Cuts at: 5 52 6454 5
Size: 47 6402 2081
Fragments arranged by size:
6402 2081 47
StuI AGG'CCT
Cuts at: 1517 2987 4236 5697 1517
Size: 1470 1249 1461 4350
Fragments arranged by size:
4350 1470 1461 1249
StyI C'CwwG_G
Cuts at: 513 1065 2004 2988 5445 5969 513
Size: 552 939 984 2457 524 3074
Fragments arranged by size:
3074 2457 984 939 552 524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6557 6574 6727 6912 7357 325
Size: 1255 4977 17 153 185 445 1498
Fragments arranged by size:
4977 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3252 3279 6776
Size: 274 80 33 51 1443 1275 27 3497
Cuts at: 6776 8488 96
Size: 1712 138
Fragments arranged by size:
3497 1712 1443 1275 274 138 80 51
33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2581 2613 5799 5831 8490 8522 2581
Size: 32 3186 32 2659 32 2589
Fragments arranged by size:
3186 2659 2589 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8530
XbaI T'CTAG_A
Cuts at: 3466 5866 3466
Size: 2400 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 8530
XhoI C'TCGA_G
Cuts at: 1943 2675 1943
Size: 732 7798
XmaI C'CCGG_G
Cuts at: 1870 5481 5855 1870
Size: 3611 374 4545
Fragments arranged by size:
4545 3611 374
XmnI GAAnn'nnTTC
Cuts at: 2256 4236 4419 5163 5676 6659 2256
Size: 1980 183 744 513 983 4127
Fragments arranged by size:
4127 1980 983 744 513 183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6338 276
Size: 53 83 186 936 4804 2468
Fragments arranged by size:
4804 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI