Restriction map for
pFN21AA1534
(Circular) (Six-base) MAPSORT of: pFN21AA1534.seq Check: 2744 from: 1 to: 7138
pFN21AA1534 7138 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2564 3214 2564
Size: 650 6488
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4948 278
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 4481 1937
Size: 136 2408 4594
Fragments arranged by size:
4594 2408 136
Acc65I G'GTAC_C
Cuts at: 3096 4459 3096
Size: 1363 5775
AclI AA'CG_TT
Cuts at: 1563 5265 5638 1563
Size: 3702 373 3063
Fragments arranged by size:
3702 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2423 2452 2945 3974 5201 6585 6853 1632
Size: 791 29 493 1029 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 1029 791 493 268 29
AfeI AGC'GCT
Cuts at: 2362 2362
Size: 7138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6941
AflIII A'CryG_T
Cuts at: 1203 3618 6037 7014 1203
Size: 2415 2419 977 1327
Fragments arranged by size:
2419 2415 1327 977
AgeI A'CCGG_T
Cuts at: 5969 5969
Size: 7138
AhdI GACnn_n'nnGTC
Cuts at: 5867 5867
Size: 7138
AleI CACnn'nnGTG
Cuts at: 1166 2213 2828 1166
Size: 1047 615 5476
Fragments arranged by size:
5476 1047 615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2663 2695 2993 3025 2663
Size: 32 298 32 6776
Fragments arranged by size:
6776 298 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2446 3694 3942 4252 4294 4881 6453 2446
Size: 1248 248 310 42 587 1572 3131
Fragments arranged by size:
3131 1572 1248 587 310 248 42
ApaI G_GGCC'C
Cuts at: 2190 2411 2806 3844 4050 2190
Size: 221 395 1038 206 5278
Fragments arranged by size:
5278 1038 395 221 206
ApaLI G'TGCA_C
Cuts at: 5195 6351 5195
Size: 1156 5982
ApoI r'AATT_y
Cuts at: 1493 3506 4447 4673 4825 6934 1493
Size: 2013 941 226 152 2109 1697
Fragments arranged by size:
2109 2013 1697 941 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5692 160
Size: 872 4660 1606
Fragments arranged by size:
4660 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
AvaI C'yCGr_G
Cuts at: 1870 1943 3556 3667 4463 1870
Size: 73 1613 111 796 4545
Fragments arranged by size:
4545 1613 796 111 73
BamHI G'GATC_C
Cuts at: 3984 4136 4468 4835 3984
Size: 152 332 367 6287
Fragments arranged by size:
6287 367 332 152
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2215 2507 2552 3096
Size: 325 217 627 241 187 292 45 544
Cuts at: 3096 3709 4302 4345 4459 5914 618
Size: 613 593 43 114 1455 1842
Fragments arranged by size:
1842 1455 627 613 593 544 325 292
241 217 187 114 45 43
BanII G_rGCy'C
Cuts at: 1391 2124 2157 2190 2411 2432 2806 3844 4050
Size: 733 33 33 221 21 374 1038 206
Cuts at: 4050 4164 4211 4325 4373 4457 1391
Size: 114 47 114 48 84 4072
Fragments arranged by size:
4072 1038 733 374 221 206 114 114
84 48 47 33 33 21
BbeI G_GCGC'C
Cuts at: 2032 2511 2032
Size: 479 6659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2670 2771 928
Size: 971 771 101 5295
Fragments arranged by size:
5295 971 771 101
BbvCI CC'TCA_GC
Cuts at: 2105 2632 3695 2105
Size: 527 1063 5548
Fragments arranged by size:
5548 1063 527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5329 5363 1903
Size: 34 3392 34 3678
Fragments arranged by size:
3678 3392 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5030 6240 5030
Size: 1210 5928
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7138
BfrBI ATG'CAT
Cuts at: 2696 2696
Size: 7138
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2406 3600 5749
Size: 107 122 71 631 605 734 1194 2149
Cuts at: 5749 136
Size: 1525
Fragments arranged by size:
2149 1525 1194 734 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3552 7133 3552
Size: 3581 3557
BlpI GC'TnA_GC
Cuts at: 2357 4319 4555 2357
Size: 1962 236 4940
Fragments arranged by size:
4940 1962 236
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2411 2555 2806 3844 4050 5199 6355
Size: 400 221 144 251 1038 206 1149 1156
Cuts at: 6355 1790
Size: 2573
Fragments arranged by size:
2573 1156 1149 1038 400 251 221 206
144
BmgBI CAC'GTC
Cuts at: 1318 3259 3570 3619 3760 1318
Size: 1941 311 49 141 4696
Fragments arranged by size:
4696 1941 311 141 49
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2558 3616 3961
Size: 416 53 83 399 24 1141 1058 345
Cuts at: 3961 4869 5827 442
Size: 908 958 1753
Fragments arranged by size:
1753 1141 1058 958 908 416 399 345
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 7138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 2705 3002 3025 3467 3737 3755 3839
Size: 1295 171 297 23 442 270 18 84
Cuts at: 3839 5798 1239
Size: 1959 2579
Fragments arranged by size:
2579 1959 1295 442 297 270 171 84
23 18
Bpu10I CC'TnA_GC
Cuts at: 2105 2632 3695 2105
Size: 527 1063 5548
Fragments arranged by size:
5548 1063 527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 3500 5263 6128 6426 2285
Size: 1215 1763 865 298 2997
Fragments arranged by size:
2997 1763 1215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 2786 3155 5801 882
Size: 617 1239 48 369 2646 2219
Fragments arranged by size:
2646 2219 1239 617 369 48
BsaAI yAC'GTr
Cuts at: 493 1204 4929 493
Size: 711 3725 2702
Fragments arranged by size:
3725 2702 711
BsaBI GATnn'nnATC
Cuts at: 7132 7132
Size: 7138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2508 2870 4945
Size: 53 83 186 936 496 479 362 2075
Cuts at: 4945 5327 5962 275
Size: 382 635 1451
Fragments arranged by size:
2075 1451 936 635 496 479 382 362
186 83 53
BsaWI w'CCGG_w
Cuts at: 3267 5571 5969 6243 6390 6801 3267
Size: 2304 398 274 147 411 3604
Fragments arranged by size:
3604 2304 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2873 2903 2993 3023 3053
Size: 30 165 30 1470 30 90 30 30
Cuts at: 3053 3567 3597 3722 3752 4088 4118 1178
Size: 514 30 125 30 336 30 4198
Fragments arranged by size:
4198 1470 514 336 165 125 90 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2093 2744 2861 2909 3071 3734 3836
Size: 841 66 651 117 48 162 663 102
Cuts at: 3836 4187 1186
Size: 351 4137
Fragments arranged by size:
4137 841 663 651 351 162 117 102
66 48
BseYI C'CCAG_C
Cuts at: 2375 2450 2503 2851 2860 3451 3498 4284 6341
Size: 75 53 348 9 591 47 786 2057
Cuts at: 6341 6837 2375
Size: 496 2676
Fragments arranged by size:
2676 2057 786 591 496 348 75 53
47 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4238 4243 4280 1163
Size: 573 2502 5 37 4021
Fragments arranged by size:
4021 2502 573 37 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4588 5349 6377 1540
Size: 275 186 2587 761 1028 2301
Fragments arranged by size:
2587 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 4164 4325 5199 5284 6355 2124
Size: 2040 161 874 85 1071 2907
Fragments arranged by size:
2907 2040 1071 874 161 85
BsmI GAATG_Cn'
Cuts at: 2696 4649 4742 2696
Size: 1953 93 5092
Fragments arranged by size:
5092 1953 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2565 2565
Size: 7138
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2157 2190 2411 2432 2555 2806
Size: 399 334 33 33 221 21 123 251
Cuts at: 2806 3844 4050 4164 4211 4325 4373 4457 5199
Size: 1038 206 114 47 114 48 84 742
Cuts at: 5199 5284 6355 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1038 742 399 334 251 221
206 123 114 114 85 84 48 47
33 33 21
BspEI T'CCGG_A
Cuts at: 6801 6801
Size: 7138
BspHI T'CATG_A
Cuts at: 5027 6757 5027
Size: 1730 5408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2564 2827 3042 3214 4493 844
Size: 1720 263 215 172 1279 3489
Fragments arranged by size:
3489 1720 1279 263 215 172
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 3672 3850 5025 1394
Size: 429 1819 30 178 1175 3507
Fragments arranged by size:
3507 1819 1175 429 178 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 5633 5807 65
Size: 1183 4385 174 1396
Fragments arranged by size:
4385 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2221 2322 5782 5969 1700
Size: 110 411 101 3460 187 2869
Fragments arranged by size:
3460 2869 411 187 110 101
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7138
BssHII G'CGCG_C
Cuts at: 1925 2338 1925
Size: 413 6725
BssSI C'ACGA_G
Cuts at: 4055 4256 5198 6210 4055
Size: 201 942 1012 4983
Fragments arranged by size:
4983 1012 942 201
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3709 4252 4294 794
Size: 2915 543 42 3638
Fragments arranged by size:
3638 2915 543 42
BstBI TT'CG_AA
Cuts at: 5942 5942
Size: 7138
BstEII G'GTnAC_C
Cuts at: 1184 2281 3463 1184
Size: 1097 1182 4859
Fragments arranged by size:
4859 1182 1097
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 4033 2830
Size: 1203 5935
BstYI r'GATC_y
Cuts at: 1972 3552 3984 4136 4468 4835 5220 5237 6689
Size: 1580 432 152 332 367 385 17 1452
Cuts at: 6689 6775 6787 7133 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1580 1452 432 385 367 346 332
152 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2727 2727
Size: 7138
BtgI C'CryG_G
Cuts at: 513 1065 4011 4122 4326 513
Size: 552 2946 111 204 3325
Fragments arranged by size:
3325 2946 552 204 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2595 3946 487
Size: 683 56 1369 1351 3679
Fragments arranged by size:
3679 1369 1351 683 56
BtsI GCAGTG_nn'
Cuts at: 3412 3655 4654 5448 5468 3412
Size: 243 999 794 20 5082
Fragments arranged by size:
5082 999 794 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7103
DraI TTT'AAA
Cuts at: 4442 4442
Size: 7138
DraIII CAC_nnn'GTG
Cuts at: 3709 3709
Size: 7138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6145 809
Size: 507 4829 1802
Fragments arranged by size:
4829 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2936 3601 4108
Size: 54 1425 50 275 18 1106 665 507
Cuts at: 4108 4311 4585 5474 7103 8
Size: 203 274 889 1629 43
Fragments arranged by size:
1629 1425 1106 889 665 507 275 274
203 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4585 1537
Size: 275 2773 4090
Fragments arranged by size:
4090 2773 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5068 1353
Size: 3715 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5725 6099 6245 387
Size: 1064 138 4136 374 146 1280
Fragments arranged by size:
4136 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 4162 4323 2122
Size: 2040 161 4937
Fragments arranged by size:
4937 2040 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2452 2534 2705 2945 3002 3025
Size: 393 791 29 82 171 240 57 23
Cuts at: 3025 3467 3737 3755 3839 3974 5201 5798 6585
Size: 442 270 18 84 135 1227 597 787
Cuts at: 6585 6853 1239
Size: 268 1524
Fragments arranged by size:
1524 1227 791 787 597 442 393 270
268 240 171 135 84 82 57 29
23 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4256 4256
Size: 7138
EcoO109I rG'GnC_Cy
Cuts at: 2186 2187 2407 2408 2586 2802 2803 3946 4046
Size: 1 220 1 178 216 1 1143 100
Cuts at: 4046 4249 2186
Size: 203 5075
Fragments arranged by size:
5075 1143 220 216 203 178 100 1
1 1
EcoRI G'AATT_C
Cuts at: 3506 4447 4825 3506
Size: 941 378 5819
Fragments arranged by size:
5819 941 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3295 3505 3541 3295
Size: 210 36 6892
Fragments arranged by size:
6892 210 36
FspI TGC'GCA
Cuts at: 5644 5644
Size: 7138
HaeII r_GCGC'y
Cuts at: 1678 2032 2364 2511 3341 6285 1678
Size: 354 332 147 830 2944 2531
Fragments arranged by size:
2944 2531 830 354 332 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 2873 2905 3023
Size: 32 135 32 1112 32 157 32 118
Cuts at: 3023 3055 3212 3244 3567 3599 3722 3754 4088
Size: 32 157 32 323 32 123 32 334
Cuts at: 4088 4120 5779 5811 5853 5885 1373
Size: 32 1659 32 42 32 2626
Fragments arranged by size:
2626 1659 1112 334 323 157 157 135
123 118 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2074 4482 4728 669
Size: 988 281 136 2408 246 3079
Fragments arranged by size:
3079 2408 988 281 246 136
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7138
HpaI GTT'AAC
Cuts at: 4728 4728
Size: 7138
KasI G'GCGC_C
Cuts at: 2028 2507 2028
Size: 479 6659
KpnI G_GTAC'C
Cuts at: 3100 4463 3100
Size: 1363 5775
MfeI C'AATT_G
Cuts at: 4737 4737
Size: 7138
MluI A'CGCG_T
Cuts at: 7014 7014
Size: 7138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 3036 6252 6436 1305
Size: 1386 345 3216 184 2007
Fragments arranged by size:
3216 2007 1386 345 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3603 10
Size: 54 1425 343 1771 3545
Fragments arranged by size:
3545 1771 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2828 5096 5455 5614 518
Size: 648 1047 615 2268 359 159 2042
Fragments arranged by size:
2268 2042 1047 648 615 359 159
MspA1I CmG'CkG
Cuts at: 1606 2109 2353 3171 3502 3715 3832 3838 3883
Size: 503 244 818 331 213 117 6 45
Cuts at: 3883 4078 4134 4414 4554 5231 6379 6624 1606
Size: 195 56 280 140 677 1148 245 2120
Fragments arranged by size:
2120 1148 818 677 503 331 280 245
244 213 195 140 117 56 45 6
NarI GG'CG_CC
Cuts at: 2029 2508 2029
Size: 479 6659
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6586
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7138
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7138
NotI GC'GGCC_GC
Cuts at: 4585 4585
Size: 7138
NsiI A_TGCA'T
Cuts at: 2698 2698
Size: 7138
NspI r_CATG'y
Cuts at: 1132 1229 4496 6041 1132
Size: 97 3267 1545 2229
Fragments arranged by size:
3267 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2138 2175 2798 3942 4043 1348
Size: 790 37 623 1144 101 4443
Fragments arranged by size:
4443 1144 790 623 101 37
PciI A'CATG_T
Cuts at: 6037 6037
Size: 7138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7138
PfoI T'CCnGG_A
Cuts at: 2841 2841
Size: 7138
PmeI GTTT'AAAC
Cuts at: 4442 4442
Size: 7138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 5207 5239 6720 6752 2993
Size: 32 2182 32 1481 32 3379
Fragments arranged by size:
3379 2182 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2586 3946 2586
Size: 1360 5778
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7138
PsiI TTA'TAA
Cuts at: 4708 4708
Size: 7138
PspOMI G'GGCC_C
Cuts at: 2186 2407 2802 3840 4046 2186
Size: 221 395 1038 206 5278
Fragments arranged by size:
5278 1038 395 221 206
PstI C_TGCA'G
Cuts at: 830 4248 4368 4490 830
Size: 3418 120 122 3478
Fragments arranged by size:
3478 3418 122 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7138
PvuII CAG'CTG
Cuts at: 2353 3502 3883 4414 2353
Size: 1149 381 531 5077
Fragments arranged by size:
5077 1149 531 381
SacI G_AGCT'C
Cuts at: 2124 4164 4325 2124
Size: 2040 161 4937
Fragments arranged by size:
4937 2040 161
SalI G'TCGA_C
Cuts at: 1936 2072 4480 1936
Size: 136 2408 4594
Fragments arranged by size:
4594 2408 136
SanDI GG'GwC_CC
Cuts at: 3946 3946
Size: 7138
SbfI CC_TGCA'GG
Cuts at: 4490 4490
Size: 7138
ScaI AGT'ACT
Cuts at: 5386 7098 5386
Size: 1712 5426
SfcI C'TryA_G
Cuts at: 826 1045 3067 4244 4364 4486 5621 6302 6493
Size: 219 2022 1177 120 122 1135 681 191
Cuts at: 6493 826
Size: 1471
Fragments arranged by size:
2022 1471 1177 1135 681 219 191 122
120
SfoI GGC'GCC
Cuts at: 2030 2509 2030
Size: 479 6659
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7138
SmaI CCC'GGG
Cuts at: 1872 4465 1872
Size: 2593 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 3515 5242 6143 6405 820
Size: 197 926 357 1215 1727 901 262 1553
Fragments arranged by size:
1727 1553 1215 926 901 357 262 197
SnaBI TAC'GTA
Cuts at: 493 4929 493
Size: 4436 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7138
SphI G_CATG'C
Cuts at: 1132 4496 1132
Size: 3364 3774
SspI AAT'ATT
Cuts at: 5 52 5062 5
Size: 47 5010 2081
Fragments arranged by size:
5010 2081 47
StuI AGG'CCT
Cuts at: 1517 2018 4359 1517
Size: 501 2341 4296
Fragments arranged by size:
4296 2341 501
StyI C'CwwG_G
Cuts at: 513 1065 3123 4577 513
Size: 552 2058 1454 3074
Fragments arranged by size:
3074 2058 1454 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2661 3443 3920 5165 5182 5335 5520
Size: 1255 1081 782 477 1245 17 153 185
Cuts at: 5520 5965 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1245 1081 782 477 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 5384 7096
Size: 274 80 33 51 1443 252 3155 1712
Cuts at: 7096 96
Size: 138
Fragments arranged by size:
3155 1712 1443 274 252 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7098 7130 7098
Size: 32 7106
Tth111I GACn'n_nGTC
Cuts at: 1316 2887 4190 1316
Size: 1571 1303 4264
Fragments arranged by size:
4264 1571 1303
XbaI T'CTAG_A
Cuts at: 4474 4474
Size: 7138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2977 3049 2977
Size: 72 7066
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7138
XmaI C'CCGG_G
Cuts at: 1870 4463 1870
Size: 2593 4545
XmnI GAAnn'nnTTC
Cuts at: 3488 5267 3488
Size: 1779 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4946 276
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI