Restriction map for
pFN21AA1531
(Circular) (Six-base) MAPSORT of: pFN21AA1531.seq Check: 540 from: 1 to: 8068
pFN21AA1531 8068 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2604 3513 2604
Size: 909 7159
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5295 5878 278
Size: 53 83 186 936 3759 583 2468
Fragments arranged by size:
3759 2468 936 583 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3435 3672 5411 1937
Size: 1498 237 1739 4594
Fragments arranged by size:
4594 1739 1498 237
Acc65I G'GTAC_C
Cuts at: 2847 5389 2847
Size: 2542 5526
AclI AA'CG_TT
Cuts at: 1563 6195 6568 1563
Size: 4632 373 3063
Fragments arranged by size:
4632 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2426 3082 6131 7515 7783 1632
Size: 794 656 3049 1384 268 1917
Fragments arranged by size:
3049 1917 1384 794 656 268
AfeI AGC'GCT
Cuts at: 4481 4481
Size: 8068
AflII C'TTAA_G
Cuts at: 820 1017 2549 820
Size: 197 1532 6339
Fragments arranged by size:
6339 1532 197
AflIII A'CryG_T
Cuts at: 1203 6967 7944 1203
Size: 5764 977 1327
Fragments arranged by size:
5764 1327 977
AgeI A'CCGG_T
Cuts at: 6899 6899
Size: 8068
AhdI GACnn_n'nnGTC
Cuts at: 6797 6797
Size: 8068
AleI CACnn'nnGTG
Cuts at: 1166 3164 3278 3293 4560 1166
Size: 1998 114 15 1267 4674
Fragments arranged by size:
4674 1998 1267 114 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3503 3704 3893 1184
Size: 2319 201 189 5359
Fragments arranged by size:
5359 2319 201 189
AlwNI CAG_nnn'CTG
Cuts at: 2689 5811 7383 2689
Size: 3122 1572 3374
Fragments arranged by size:
3374 3122 1572
ApaI G_GGCC'C
Cuts at: 2620 2731 4368 4648 4695 4751 2620
Size: 111 1637 280 47 56 5937
Fragments arranged by size:
5937 1637 280 111 56 47
ApaLI G'TGCA_C
Cuts at: 2986 4880 6125 7281 2986
Size: 1894 1245 1156 3773
Fragments arranged by size:
3773 1894 1245 1156
ApoI r'AATT_y
Cuts at: 1493 3419 5377 5603 5755 7864 1493
Size: 1926 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1926 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2103 4594 2103
Size: 2491 5577
AseI AT'TA_AT
Cuts at: 160 1032 6622 160
Size: 872 5590 1606
Fragments arranged by size:
5590 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8068
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2097 2143 2234 3187 3195 4361
Size: 73 147 7 46 91 953 8 1166
Cuts at: 4361 4491 4641 4845 5065 5393 1870
Size: 130 150 204 220 328 4545
Fragments arranged by size:
4545 1166 953 328 220 204 150 147
130 91 73 46 8 7
BamHI G'GATC_C
Cuts at: 5398 5765 5398
Size: 367 7701
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2035 2086 2258 2381
Size: 325 217 627 222 26 51 172 123
Cuts at: 2381 2567 2847 3182 3191 3554 3644 3955 4130
Size: 186 280 335 9 363 90 311 175
Cuts at: 4130 4841 5069 5264 5389 6844 618
Size: 711 228 195 125 1455 1842
Fragments arranged by size:
1842 1455 711 627 363 335 325 311
280 228 222 217 195 186 175 172
125 123 90 51 26 9
BanII G_rGCy'C
Cuts at: 1391 2241 2301 2620 2731 2863 3945 4368 4465
Size: 850 60 319 111 132 1082 423 97
Cuts at: 4465 4648 4695 4751 5097 5387 1391
Size: 183 47 56 346 290 4072
Fragments arranged by size:
4072 1082 850 423 346 319 290 183
132 111 97 60 56 47
BbeI G_GCGC'C
Cuts at: 2013 2039 2090 2385 3648 4134 5073 5268 2013
Size: 26 51 295 1263 486 939 195 4813
Fragments arranged by size:
4813 1263 939 486 295 195 51 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4540 5342 928
Size: 971 2641 802 3654
Fragments arranged by size:
3654 2641 971 802
BbvCI CC'TCA_GC
Cuts at: 3584 4271 3584
Size: 687 7381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3059 3093 3529 3563 6259 6293 1903
Size: 34 1122 34 436 34 2696 34 3678
Fragments arranged by size:
3678 2696 1122 436 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3162 5960 7170 3162
Size: 2798 1210 4060
Fragments arranged by size:
4060 2798 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8068
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2161 4451 4460
Size: 107 122 71 631 605 489 2290 9
Cuts at: 4460 5160 5262 6679 136
Size: 700 102 1417 1525
Fragments arranged by size:
2290 1525 1417 700 631 605 489 122
107 102 71 9
BglII A'GATC_T
Cuts at: 2416 8063 2416
Size: 5647 2421
BlpI GC'TnA_GC
Cuts at: 2165 3617 5485 2165
Size: 1452 1868 4748
Fragments arranged by size:
4748 1868 1452
Bme1580I G_kGCm'C
Cuts at: 1790 2570 2620 2674 2731 2990 3171 3187 3194
Size: 780 50 54 57 259 181 16 7
Cuts at: 3194 3559 3958 4368 4648 4695 4751 4844 4884
Size: 365 399 410 280 47 56 93 40
Cuts at: 4884 6129 7285 1790
Size: 1245 1156 2573
Fragments arranged by size:
2573 1245 1156 780 410 399 365 280
259 181 93 57 56 54 50 47
40 16 7
BmgBI CAC'GTC
Cuts at: 1318 4579 1318
Size: 3261 4807
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2661 3990 4347
Size: 416 53 83 399 24 1244 1329 357
Cuts at: 4347 5799 6757 442
Size: 1452 958 1753
Fragments arranged by size:
1753 1452 1329 1244 958 416 399 357
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3372 1056
Size: 2316 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2494 2684 2753 3062 3581 3970 4047
Size: 1115 140 190 69 309 519 389 77
Cuts at: 4047 4274 5009 5231 6728 1239
Size: 227 735 222 1497 2579
Fragments arranged by size:
2579 1497 1115 735 519 389 309 227
222 190 140 77 69
Bpu10I CC'TnA_GC
Cuts at: 2682 3584 4271 4289 2682
Size: 902 687 18 6461
Fragments arranged by size:
6461 902 687 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 4688 4940 5177 6193 7058 7356 2296
Size: 2392 252 237 1016 865 298 3008
Fragments arranged by size:
3008 2392 1016 865 298 252 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2447 3038 6731 882
Size: 617 948 591 3693 2219
Fragments arranged by size:
3693 2219 948 617 591
BsaAI yAC'GTr
Cuts at: 493 1204 5859 493
Size: 711 4655 2702
Fragments arranged by size:
4655 2702 711
BsaBI GATnn'nnATC
Cuts at: 3305 8062 3305
Size: 4757 3311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2036 2087 2175
Size: 53 83 186 936 477 26 51 88
Cuts at: 2175 2382 3645 4117 4131 4358 4891 5070 5265
Size: 207 1263 472 14 227 533 179 195
Cuts at: 5265 5292 5875 6257 6892 275
Size: 27 583 382 635 1451
Fragments arranged by size:
1451 1263 936 635 583 533 477 472
382 227 207 195 186 179 88 83
53 51 27 26 14
BsaWI w'CCGG_w
Cuts at: 2430 4008 5339 6501 6899 7173 7320 7731 2430
Size: 1578 1331 1162 398 274 147 411 2767
Fragments arranged by size:
2767 1578 1331 1162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 3725 3755 3872
Size: 30 165 30 1089 30 1203 30 117
Cuts at: 3872 3902 4982 5012 1178
Size: 30 1080 30 4234
Fragments arranged by size:
4234 1203 1089 1080 165 117 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3877 4280 1186
Size: 2691 403 4974
Fragments arranged by size:
4974 2691 403
BseYI C'CCAG_C
Cuts at: 2709 3228 3519 3597 4476 7271 7767 2709
Size: 519 291 78 879 2795 496 3010
Fragments arranged by size:
3010 2795 879 519 496 291 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2227 2393 2723 2905 3644 3664 3863
Size: 573 491 166 330 182 739 20 199
Cuts at: 3863 4522 5003 1163
Size: 659 481 4228
Fragments arranged by size:
4228 739 659 573 491 481 330 199
182 166 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 4812 5518 6279 7307 1540
Size: 275 186 1225 1586 706 761 1028 2301
Fragments arranged by size:
2301 1586 1225 1028 761 706 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 2810 2990 3504 3933 3945 4884 6129 6214
Size: 666 180 514 429 12 939 1245 85
Cuts at: 6214 7285 2144
Size: 1071 2927
Fragments arranged by size:
2927 1245 1071 939 666 514 429 180
85 12
BsmI GAATG_Cn'
Cuts at: 5579 5672 5579
Size: 93 7975
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2241 2301 2570 2620 2674 2731
Size: 399 354 97 60 269 50 54 57
Cuts at: 2731 2810 2863 2990 3171 3187 3194 3504 3559
Size: 79 53 127 181 16 7 310 55
Cuts at: 3559 3933 3945 3958 4368 4465 4648 4695 4751
Size: 374 12 13 410 97 183 47 56
Cuts at: 4751 4844 4884 5097 5387 6129 6214 7285 1391
Size: 93 40 213 290 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 374 354 310
290 269 213 183 181 127 97 97
93 85 79 60 57 56 55 54
53 50 47 40 16 13 12 7
BspEI T'CCGG_A
Cuts at: 7731 7731
Size: 8068
BspHI T'CATG_A
Cuts at: 5957 7687 5957
Size: 1730 6338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2604 3513 4776 5423 844
Size: 1760 909 1263 647 3489
Fragments arranged by size:
3489 1760 1263 909 647
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 2169 3983 5010 5232 5242 5955
Size: 429 329 17 1814 1027 222 10 713
Cuts at: 5955 1394
Size: 3507
Fragments arranged by size:
3507 1814 1027 713 429 329 222 17
10
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 6563 6737 65
Size: 1183 1063 4252 174 1396
Fragments arranged by size:
4252 1396 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3207 3221 4811 4834 4950 5072 6712
Size: 110 1397 14 1590 23 116 122 1640
Cuts at: 6712 6899 1700
Size: 187 2869
Fragments arranged by size:
2869 1640 1590 1397 187 122 116 110
23 14
BsrGI T'GTAC_A
Cuts at: 96 3285 3351 96
Size: 3189 66 4813
Fragments arranged by size:
4813 3189 66
BssHII G'CGCG_C
Cuts at: 1925 2042 2103 4344 4594 5205 1925
Size: 117 61 2241 250 611 4788
Fragments arranged by size:
4788 2241 611 250 117 61
BssSI C'ACGA_G
Cuts at: 4588 6128 7140 4588
Size: 1540 1012 5516
Fragments arranged by size:
5516 1540 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3775 794
Size: 2981 5087
BstBI TT'CG_AA
Cuts at: 6872 6872
Size: 8068
BstEII G'GTnAC_C
Cuts at: 1184 4420 1184
Size: 3236 4832
BstXI CCAn_nnnn'nTGG
Cuts at: 2821 3637 2821
Size: 816 7252
BstYI r'GATC_y
Cuts at: 1972 2416 4173 5398 5765 6150 6167 7619 7705
Size: 444 1757 1225 367 385 17 1452 86
Cuts at: 7705 7717 8063 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1757 1452 1225 444 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3673 3673
Size: 8068
Bsu36I CC'TnA_GG
Cuts at: 2348 2971 2348
Size: 623 7445
BtgI C'CryG_G
Cuts at: 513 1065 2004 2066 2997 3088 3666 4620 4885
Size: 552 939 62 931 91 578 954 265
Cuts at: 4885 513
Size: 3696
Fragments arranged by size:
3696 954 939 931 578 552 265 91
62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5210 487
Size: 683 56 3984 3345
Fragments arranged by size:
3984 3345 683 56
BtsI GCAGTG_nn'
Cuts at: 2327 4476 5584 6378 6398 2327
Size: 2149 1108 794 20 3997
Fragments arranged by size:
3997 2149 1108 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8068
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8033
DraI TTT'AAA
Cuts at: 5372 5372
Size: 8068
DraIII CAC_nnn'GTG
Cuts at: 3667 3667
Size: 8068
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7075 809
Size: 507 5759 1802
Fragments arranged by size:
5759 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2898 3223 3474
Size: 54 1425 50 275 18 1068 325 251
Cuts at: 3474 4443 4729 4809 5515 6404 8033 8
Size: 969 286 80 706 889 1629 43
Fragments arranged by size:
1629 1425 1068 969 889 706 325 286
275 251 80 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 4809 5515 1537
Size: 275 1411 1586 706 4090
Fragments arranged by size:
4090 1586 1411 706 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3710 4547 5345 5998 1353
Size: 2357 837 798 653 3423
Fragments arranged by size:
3423 2357 837 798 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2209 4519 4585 6655 7029 7175
Size: 1064 138 620 2310 66 2070 374 146
Cuts at: 7175 387
Size: 1280
Fragments arranged by size:
2310 2070 1280 1064 620 374 146 138
66
EcoICRI GAG'CTC
Cuts at: 3943 3943
Size: 8068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2426 2494 2684 2753 3062 3082
Size: 393 722 72 68 190 69 309 20
Cuts at: 3082 3581 3970 4047 4274 5009 5231 6131 6728
Size: 499 389 77 227 735 222 900 597
Cuts at: 6728 7515 7783 1239
Size: 787 268 1524
Fragments arranged by size:
1524 900 787 735 722 597 499 393
389 309 268 227 222 190 77 72
69 68 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 3628 2702
Size: 926 7142
EcoO109I rG'GnC_Cy
Cuts at: 2218 2464 2686 2727 2728 3976 4208 4245 4365
Size: 246 222 41 1 1248 232 37 120
Cuts at: 4365 4645 4691 4692 4748 4960 2218
Size: 280 46 1 56 212 5326
Fragments arranged by size:
5326 1248 280 246 232 222 212 120
56 46 41 37 1 1
EcoRI G'AATT_C
Cuts at: 5377 5755 5377
Size: 378 7690
FspI TGC'GCA
Cuts at: 6574 6574
Size: 8068
HaeII r_GCGC'y
Cuts at: 1678 2013 2039 2090 2385 3624 3648 4134 4418
Size: 335 26 51 295 1239 24 486 284
Cuts at: 4418 4483 4988 5073 5133 5170 5178 5268 7215
Size: 65 505 85 60 37 8 90 1947
Cuts at: 7215 1678
Size: 2531
Fragments arranged by size:
2531 1947 1239 505 486 335 295 284
90 85 65 60 51 37 26 24
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2425 2457 2935 2967 3319
Size: 32 135 32 853 32 478 32 352
Cuts at: 3319 3351 4982 4999 5014 5031 6709 6741 6783
Size: 32 1631 17 15 17 1678 32 42
Cuts at: 6783 6815 1373
Size: 32 2626
Fragments arranged by size:
2626 1678 1631 853 478 352 135 42
32 32 32 32 32 32 32 17
17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3436 4069 5412 5658 669
Size: 988 281 1498 633 1343 246 3079
Fragments arranged by size:
3079 1498 1343 988 633 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8068
HpaI GTT'AAC
Cuts at: 5658 5658
Size: 8068
KasI G'GCGC_C
Cuts at: 2009 2035 2086 2381 3644 4130 5069 5264 2009
Size: 26 51 295 1263 486 939 195 4813
Fragments arranged by size:
4813 1263 939 486 295 195 51 26
KpnI G_GTAC'C
Cuts at: 2851 5393 2851
Size: 2542 5526
MfeI C'AATT_G
Cuts at: 5667 5667
Size: 8068
MluI A'CGCG_T
Cuts at: 7944 7944
Size: 8068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2448 2506 2622 7182 7366 1305
Size: 1143 58 116 4560 184 2007
Fragments arranged by size:
4560 2007 1143 184 116 58
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3476 4445 10
Size: 54 1425 343 1644 969 3633
Fragments arranged by size:
3633 1644 1425 969 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2960 3164 3278 3293 3608 3749 3800
Size: 648 1794 204 114 15 315 141 51
Cuts at: 3800 4145 4560 5300 6026 6385 6544 518
Size: 345 415 740 726 359 159 2042
Fragments arranged by size:
2042 1794 740 726 648 415 359 345
315 204 159 141 114 51 15
MspA1I CmG'CkG
Cuts at: 1606 2059 2133 2211 2638 2844 3090 3228 3343
Size: 453 74 78 427 206 246 138 115
Cuts at: 3343 4061 4299 4506 4622 4728 4738 4774 5083
Size: 718 238 207 116 106 10 36 309
Cuts at: 5083 5322 5484 6161 7309 7554 1606
Size: 239 162 677 1148 245 2120
Fragments arranged by size:
2120 1148 718 677 453 427 309 246
245 239 238 207 206 162 138 116
115 106 78 74 36 10
NaeI GCC'GGC
Cuts at: 3223 4813 4836 5074 3223
Size: 1590 23 238 6217
Fragments arranged by size:
6217 1590 238 23
NarI GG'CG_CC
Cuts at: 2010 2036 2087 2382 3645 4131 5070 5265 2010
Size: 26 51 295 1263 486 939 195 4813
Fragments arranged by size:
4813 1263 939 486 295 195 51 26
NcoI C'CATG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 5584
Fragments arranged by size:
5584 993 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8068
NgoMIV G'CCGG_C
Cuts at: 3221 4811 4834 5072 3221
Size: 1590 23 238 6217
Fragments arranged by size:
6217 1590 238 23
NheI G'CTAG_C
Cuts at: 1052 3368 1052
Size: 2316 5752
NotI GC'GGCC_GC
Cuts at: 5515 5515
Size: 8068
NspI r_CATG'y
Cuts at: 1132 1229 2681 3775 5426 6971 1132
Size: 97 1452 1094 1651 1545 2229
Fragments arranged by size:
2229 1651 1545 1452 1094 97
PasI CC'CwG_GG
Cuts at: 1348 2613 2689 3172 3630 4534 1348
Size: 1265 76 483 458 904 4882
Fragments arranged by size:
4882 1265 904 483 458 76
PciI A'CATG_T
Cuts at: 6967 6967
Size: 8068
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3865 4127 4738 1108
Size: 2757 262 611 4438
Fragments arranged by size:
4438 2757 611 262
PfoI T'CCnGG_A
Cuts at: 2223 2700 3755 4396 2223
Size: 477 1055 641 5895
Fragments arranged by size:
5895 1055 641 477
PmeI GTTT'AAAC
Cuts at: 5372 5372
Size: 8068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2637 2669 6137 6169 7650 7682 2637
Size: 32 3468 32 1481 32 3023
Fragments arranged by size:
3468 3023 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 3976 4208 2218
Size: 1758 232 6078
Fragments arranged by size:
6078 1758 232
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8068
PsiI TTA'TAA
Cuts at: 5638 5638
Size: 8068
PspOMI G'GGCC_C
Cuts at: 2616 2727 4364 4644 4691 4747 2616
Size: 111 1637 280 47 56 5937
Fragments arranged by size:
5937 1637 280 111 56 47
PstI C_TGCA'G
Cuts at: 830 2637 3150 3542 4066 4773 5082 5420 830
Size: 1807 513 392 524 707 309 338 3478
Fragments arranged by size:
3478 1807 707 524 513 392 338 309
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8068
PvuII CAG'CTG
Cuts at: 2133 2638 3343 4061 4506 4774 5083 2133
Size: 505 705 718 445 268 309 5118
Fragments arranged by size:
5118 718 705 505 445 309 268
SacI G_AGCT'C
Cuts at: 3945 3945
Size: 8068
SacII CC_GC'GG
Cuts at: 3091 4623 3091
Size: 1532 6536
SalI G'TCGA_C
Cuts at: 1936 3434 5410 1936
Size: 1498 1976 4594
Fragments arranged by size:
4594 1976 1498
SanDI GG'GwC_CC
Cuts at: 3976 3976
Size: 8068
SapI GCTCTTCn'nnn_
Cuts at: 5345 5345
Size: 8068
SbfI CC_TGCA'GG
Cuts at: 5420 5420
Size: 8068
ScaI AGT'ACT
Cuts at: 6316 8028 6316
Size: 1712 6356
SfcI C'TryA_G
Cuts at: 826 1045 2633 3146 3538 4062 4769 5078 5416
Size: 219 1588 513 392 524 707 309 338
Cuts at: 5416 6551 7232 7423 826
Size: 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 707 681 524 513 392
338 309 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4451 5160 4451
Size: 709 7359
SfoI GGC'GCC
Cuts at: 2011 2037 2088 2383 3646 4132 5071 5266 2011
Size: 26 51 295 1263 486 939 195 4813
Fragments arranged by size:
4813 1263 939 486 295 195 51 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8068
SgrAI Cr'CCGG_yG
Cuts at: 1700 3207 1700
Size: 1507 6561
SmaI CCC'GGG
Cuts at: 1872 3189 4363 4493 4643 4847 5067 5395 1872
Size: 1317 1174 130 150 204 220 328 4545
Fragments arranged by size:
4545 1317 1174 328 220 204 150 130
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2275 2549 4703 4955 5192 6172
Size: 197 926 332 274 2154 252 237 980
Cuts at: 6172 7073 7335 820
Size: 901 262 1553
Fragments arranged by size:
2154 1553 980 926 901 332 274 262
252 237 197
SnaBI TAC'GTA
Cuts at: 493 5859 493
Size: 5366 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8068
SphI G_CATG'C
Cuts at: 1132 2681 5426 1132
Size: 1549 2745 3774
Fragments arranged by size:
3774 2745 1549
SrfI GCCC'GGGC
Cuts at: 4363 5067 4363
Size: 704 7364
SspI AAT'ATT
Cuts at: 5 52 5992 5
Size: 47 5940 2081
Fragments arranged by size:
5940 2081 47
StuI AGG'CCT
Cuts at: 1517 3536 4937 1517
Size: 2019 1401 4648
Fragments arranged by size:
4648 2019 1401
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2997 3004 4126 4227 4914
Size: 552 939 153 840 7 1122 101 687
Cuts at: 4914 5271 5507 513
Size: 357 236 3074
Fragments arranged by size:
3074 1122 939 840 687 552 357 236
153 101 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 6095 6112 6265 6450 6895 325
Size: 1255 1962 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3285 3351 4437
Size: 274 80 33 51 1443 1308 66 1086
Cuts at: 4437 6314 8026 96
Size: 1877 1712 138
Fragments arranged by size:
1877 1712 1443 1308 1086 274 138 80
66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3872 3904 8028 8060 3086
Size: 32 754 32 4124 32 3094
Fragments arranged by size:
4124 3094 754 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2947 5291 1316
Size: 1631 2344 4093
Fragments arranged by size:
4093 2344 1631
XbaI T'CTAG_A
Cuts at: 5404 5404
Size: 8068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 3847 4771 3022
Size: 825 924 6319
Fragments arranged by size:
6319 924 825
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8068
XmaI C'CCGG_G
Cuts at: 1870 3187 4361 4491 4641 4845 5065 5393 1870
Size: 1317 1174 130 150 204 220 328 4545
Fragments arranged by size:
4545 1317 1174 328 220 204 150 130
XmnI GAAnn'nnTTC
Cuts at: 6197 6197
Size: 8068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5293 5876 276
Size: 53 83 186 936 3759 583 2468
Fragments arranged by size:
3759 2468 936 583 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BplI BsiWI BsmBI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SexAI SwaI