Restriction map for
pFN21AA1526
(Circular) (Six-base) MAPSORT of: pFN21AA1526.seq Check: 96 from: 1 to: 7426
pFN21AA1526 7426 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3309 3309
Size: 7426
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3081 4209 5236 278
Size: 53 83 186 936 1545 1128 1027 2468
Fragments arranged by size:
2468 1545 1128 1027 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4769 1937
Size: 2832 4594
Acc65I G'GTAC_C
Cuts at: 4747 4747
Size: 7426
AclI AA'CG_TT
Cuts at: 1563 5553 5926 1563
Size: 3990 373 3063
Fragments arranged by size:
3990 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2449 2648 2964 5489 6873 7141 1632
Size: 817 199 316 2525 1384 268 1917
Fragments arranged by size:
2525 1917 1384 817 316 268 199
AfeI AGC'GCT
Cuts at: 2139 2139
Size: 7426
AflII C'TTAA_G
Cuts at: 820 1017 3041 820
Size: 197 2024 5205
Fragments arranged by size:
5205 2024 197
AflIII A'CryG_T
Cuts at: 1203 2505 6325 7302 1203
Size: 1302 3820 977 1327
Fragments arranged by size:
3820 1327 1302 977
AgeI A'CCGG_T
Cuts at: 6257 6257
Size: 7426
AhdI GACnn_n'nnGTC
Cuts at: 2026 2552 2866 6155 2026
Size: 526 314 3289 3297
Fragments arranged by size:
3297 3289 526 314
AleI CACnn'nnGTG
Cuts at: 1166 2711 2769 2927 3107 4566 1166
Size: 1545 58 158 180 1459 4026
Fragments arranged by size:
4026 1545 1459 180 158 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4397 1184
Size: 3213 4213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3118 3150 3118
Size: 32 7394
AlwNI CAG_nnn'CTG
Cuts at: 2569 2942 3730 4100 4522 5169 6741 2569
Size: 373 788 370 422 647 1572 3254
Fragments arranged by size:
3254 1572 788 647 422 373 370
ApaI G_GGCC'C
Cuts at: 3227 3227
Size: 7426
ApaLI G'TGCA_C
Cuts at: 5483 6639 5483
Size: 1156 6270
ApoI r'AATT_y
Cuts at: 1493 4735 4961 5113 7222 1493
Size: 3242 226 152 2109 1697
Fragments arranged by size:
3242 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5980 160
Size: 872 4948 1606
Fragments arranged by size:
4948 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7426
AvaI C'yCGr_G
Cuts at: 1870 1943 2595 2746 3536 3853 4751 1870
Size: 73 652 151 790 317 898 4545
Fragments arranged by size:
4545 898 790 652 317 151 73
AvrII C'CTAG_G
Cuts at: 4526 4526
Size: 7426
BamHI G'GATC_C
Cuts at: 4756 5123 4756
Size: 367 7059
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 2771 3054 3297 3446
Size: 325 217 627 481 503 283 243 149
Cuts at: 3446 3602 3899 4502 4747 6202 618
Size: 156 297 603 245 1455 1842
Fragments arranged by size:
1842 1455 627 603 503 481 325 297
283 245 243 217 156 149
BanII G_rGCy'C
Cuts at: 1391 2039 2063 2483 2636 2964 3227 4440 4745
Size: 648 24 420 153 328 263 1213 305
Cuts at: 4745 1391
Size: 4072
Fragments arranged by size:
4072 1213 648 420 328 305 263 153
24
BbeI G_GCGC'C
Cuts at: 2272 4506 2272
Size: 2234 5192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2209 3856 928
Size: 971 310 1647 4498
Fragments arranged by size:
4498 1647 971 310
BbvCI CC'TCA_GC
Cuts at: 3491 3491
Size: 7426
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2200 2234 4474 4508 5617 5651 1903
Size: 34 263 34 2240 34 1109 34 3678
Fragments arranged by size:
3678 2240 1109 263 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5318 6528 5318
Size: 1210 6216
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7426
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2621 3231 3329
Size: 107 122 71 631 605 949 610 98
Cuts at: 3329 4010 4097 6037 136
Size: 681 87 1940 1525
Fragments arranged by size:
1940 1525 949 681 631 610 605 122
107 98 87 71
BglII A'GATC_T
Cuts at: 7421 7421
Size: 7426
BlpI GC'TnA_GC
Cuts at: 2153 4555 4843 2153
Size: 2402 288 4736
Fragments arranged by size:
4736 2402 288
Bme1580I G_kGCm'C
Cuts at: 1790 2776 3227 3860 3902 5487 6643 1790
Size: 986 451 633 42 1585 1156 2573
Fragments arranged by size:
2573 1585 1156 986 633 451 42
BmgBI CAC'GTC
Cuts at: 1318 4443 1318
Size: 3125 4301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5157 6115 442
Size: 416 53 83 399 24 3740 958 1753
Fragments arranged by size:
3740 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7426
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3839
Size: 7426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3302 3665 3809 4454 6086 1239
Size: 2063 363 144 645 1632 2579
Fragments arranged by size:
2579 2063 1632 645 363 144
Bpu10I CC'TnA_GC
Cuts at: 3248 3491 3248
Size: 243 7183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2948 4562 5551 6416 6714 2948
Size: 1614 989 865 298 3660
Fragments arranged by size:
3660 1614 989 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3110 3143 3568 6089 882
Size: 617 1611 33 425 2521 2219
Fragments arranged by size:
2521 2219 1611 617 425 33
BsaAI yAC'GTr
Cuts at: 493 1204 2506 4591 5217 493
Size: 711 1302 2085 626 2702
Fragments arranged by size:
2702 2085 1302 711 626
BsaBI GATnn'nnATC
Cuts at: 7420 7420
Size: 7426
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2269 3078 4002 4206
Size: 53 83 186 936 736 809 924 204
Cuts at: 4206 4503 5233 5615 6250 275
Size: 297 730 382 635 1451
Fragments arranged by size:
1451 936 924 809 736 730 635 382
297 204 186 83 53
BsaWI w'CCGG_w
Cuts at: 2257 2796 5859 6257 6531 6678 7089 2257
Size: 539 3063 398 274 147 411 2594
Fragments arranged by size:
3063 2594 539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3539 3569 3590 3620 3968
Size: 30 165 30 2136 30 21 30 348
Cuts at: 3968 3998 4427 4457 1178
Size: 30 429 30 4147
Fragments arranged by size:
4147 2136 429 348 165 30 30 30
30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2815 3299 3419 3913 1186
Size: 1629 484 120 494 4699
Fragments arranged by size:
4699 1629 494 484 120
BseYI C'CCAG_C
Cuts at: 2056 2757 2854 3226 3542 3808 3980 4100 4257
Size: 701 97 372 316 266 172 120 157
Cuts at: 4257 6629 7125 2056
Size: 2372 496 2357
Fragments arranged by size:
2372 2357 701 496 372 316 266 172
157 120 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 2795 3912 1163
Size: 573 378 681 1117 4677
Fragments arranged by size:
4677 1117 681 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 2337 2866 4876 5637 6665
Size: 275 186 69 267 529 2010 761 1028
Cuts at: 6665 1540
Size: 2301
Fragments arranged by size:
2301 2010 1028 761 529 275 267 186
69
BsiHKAI G_wGCw'C
Cuts at: 2039 2490 2807 4635 5487 5572 6643 2039
Size: 451 317 1828 852 85 1071 2822
Fragments arranged by size:
2822 1828 1071 852 451 317 85
BsiWI C'GTAC_G
Cuts at: 2119 2119
Size: 7426
BsmI GAATG_Cn'
Cuts at: 4937 5030 4937
Size: 93 7333
BsmBI CGTCTCn'nnnn_
Cuts at: 3370 3970 3370
Size: 600 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2063 2483 2490 2636 2776 2807
Size: 399 249 24 420 7 146 140 31
Cuts at: 2807 2964 3227 3860 3902 4440 4635 4745 5487
Size: 157 263 633 42 538 195 110 742
Cuts at: 5487 5572 6643 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 633 538 420 399 263
249 195 157 146 140 110 85 42
31 24 7
BspEI T'CCGG_A
Cuts at: 7089 7089
Size: 7426
BspHI T'CATG_A
Cuts at: 5315 7045 5315
Size: 1730 5696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 3309 4781 844
Size: 1514 951 1472 3489
Fragments arranged by size:
3489 1514 1472 951
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 5313 1394
Size: 429 344 3146 3507
Fragments arranged by size:
3507 3146 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 5921 6095 65
Size: 1183 4673 174 1396
Fragments arranged by size:
4673 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2865 3230 6070 6257 1700
Size: 110 238 817 365 2840 187 2869
Fragments arranged by size:
2869 2840 817 365 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7426
BssHII G'CGCG_C
Cuts at: 1925 2204 1925
Size: 279 7147
BssSI C'ACGA_G
Cuts at: 2450 3270 5486 6498 2450
Size: 820 2216 1012 3378
Fragments arranged by size:
3378 2216 1012 820
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2818 794
Size: 2024 5402
BstBI TT'CG_AA
Cuts at: 6230 6230
Size: 7426
BstEII G'GTnAC_C
Cuts at: 1184 2599 2792 3241 1184
Size: 1415 193 449 5369
Fragments arranged by size:
5369 1415 449 193
BstXI CCAn_nnnn'nTGG
Cuts at: 2644 2644
Size: 7426
BstYI r'GATC_y
Cuts at: 1972 3713 4756 5123 5508 5525 6977 7063 7075
Size: 1741 1043 367 385 17 1452 86 12
Cuts at: 7075 7421 1972
Size: 346 1977
Fragments arranged by size:
1977 1741 1452 1043 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2777 2777
Size: 7426
BtgI C'CryG_G
Cuts at: 513 1065 2473 2764 2887 3153 3327 3352 3558
Size: 552 1408 291 123 266 174 25 206
Cuts at: 3558 3598 3699 4046 513
Size: 40 101 347 3893
Fragments arranged by size:
3893 1408 552 347 291 266 206 174
123 101 40 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4471 487
Size: 683 56 3245 3442
Fragments arranged by size:
3442 3245 683 56
BtsI GCAGTG_nn'
Cuts at: 2182 3746 4942 5736 5756 2182
Size: 1564 1196 794 20 3852
Fragments arranged by size:
3852 1564 1196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7391
DraI TTT'AAA
Cuts at: 4730 4730
Size: 7426
DraIII CAC_nnn'GTG
Cuts at: 2494 3487 2494
Size: 993 6433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2258 3029 4700 6433 809
Size: 507 942 771 1671 1733 1802
Fragments arranged by size:
1802 1733 1671 942 771 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2334 2863
Size: 54 1425 50 275 18 237 267 529
Cuts at: 2863 3624 4132 4473 4873 5762 7391 8
Size: 761 508 341 400 889 1629 43
Fragments arranged by size:
1629 1425 889 761 529 508 400 341
275 267 237 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2067 2334 2863 4873 1537
Size: 275 255 267 529 2010 4090
Fragments arranged by size:
4090 2010 529 275 267 255
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 3949 5356 1353
Size: 971 1625 1407 3423
Fragments arranged by size:
3423 1625 1407 971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2177 3013 6013 6387 6533 387
Size: 1064 138 588 836 3000 374 146 1280
Fragments arranged by size:
3000 1280 1064 836 588 374 146 138
EcoICRI GAG'CTC
Cuts at: 2037 2037
Size: 7426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2449 2648 2964 3302 3665 3809 4454
Size: 393 817 199 316 338 363 144 645
Cuts at: 4454 5489 6086 6873 7141 1239
Size: 1035 597 787 268 1524
Fragments arranged by size:
1524 1035 817 787 645 597 393 363
338 316 268 199 144
EcoNI CCTnn'n_nnAGG
Cuts at: 3434 3434
Size: 7426
EcoO109I rG'GnC_Cy
Cuts at: 2341 3223 3376 3533 3820 3918 2341
Size: 882 153 157 287 98 5849
Fragments arranged by size:
5849 882 287 157 153 98
EcoRI G'AATT_C
Cuts at: 4735 5113 4735
Size: 378 7048
FspI TGC'GCA
Cuts at: 2231 5932 2231
Size: 3701 3725
FspAI rTGC'GCAy
Cuts at: 2231 2231
Size: 7426
HaeII r_GCGC'y
Cuts at: 1678 2141 2272 4226 4329 4506 6573 1678
Size: 463 131 1954 103 177 2067 2531
Fragments arranged by size:
2531 2067 1954 463 177 131 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2538 2570 2852
Size: 32 135 32 440 32 494 32 282
Cuts at: 2852 2884 2924 2956 3491 3523 3539 3571 3698
Size: 32 40 32 535 32 16 32 127
Cuts at: 3698 3730 3926 3958 4427 4459 6067 6099 6141
Size: 32 196 32 469 32 1608 32 42
Cuts at: 6141 6173 1373
Size: 32 2626
Fragments arranged by size:
2626 1608 535 494 469 440 282 196
135 127 42 40 32 32 32 32
32 32 32 32 32 32 32 32
32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 4021 4770 5016 669
Size: 988 281 640 1443 749 246 3079
Fragments arranged by size:
3079 1443 988 749 640 281 246
HindIII A'AGCT_T
Cuts at: 748 3736 748
Size: 2988 4438
HpaI GTT'AAC
Cuts at: 5016 5016
Size: 7426
KasI G'GCGC_C
Cuts at: 2268 4502 2268
Size: 2234 5192
KpnI G_GTAC'C
Cuts at: 4751 4751
Size: 7426
MfeI C'AATT_G
Cuts at: 5025 5025
Size: 7426
MluI A'CGCG_T
Cuts at: 7302 7302
Size: 7426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 6540 6724 1305
Size: 1026 4209 184 2007
Fragments arranged by size:
4209 2007 1026 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4134 10
Size: 54 1425 343 2302 3302
Fragments arranged by size:
3302 2302 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 2711 2769 2927 3107 4566 5384
Size: 648 1338 207 58 158 180 1459 818
Cuts at: 5384 5743 5902 518
Size: 359 159 2042
Fragments arranged by size:
2042 1459 1338 818 648 359 207 180
159 158 58
MspA1I CmG'CkG
Cuts at: 1606 2017 2128 2314 2958 3896 3997 4272 4842
Size: 411 111 186 644 938 101 275 570
Cuts at: 4842 5519 6667 6912 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 644 570 411 275
245 186 111 101
NaeI GCC'GGC
Cuts at: 3232 3232
Size: 7426
NarI GG'CG_CC
Cuts at: 2269 4503 2269
Size: 2234 5192
NcoI C'CATG_G
Cuts at: 513 1065 3153 3327 3558 513
Size: 552 2088 174 231 4381
Fragments arranged by size:
4381 2088 552 231 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7426
NgoMIV G'CCGG_C
Cuts at: 3230 3230
Size: 7426
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7426
NotI GC'GGCC_GC
Cuts at: 4873 4873
Size: 7426
NspI r_CATG'y
Cuts at: 1132 1229 4784 6329 1132
Size: 97 3555 1545 2229
Fragments arranged by size:
3555 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2678 2872 3724 3914 4480 1348
Size: 1330 194 852 190 566 4294
Fragments arranged by size:
4294 1330 852 566 194 190
PciI A'CATG_T
Cuts at: 6325 6325
Size: 7426
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7426
PfoI T'CCnGG_A
Cuts at: 3137 3137
Size: 7426
PmeI GTTT'AAAC
Cuts at: 4730 4730
Size: 7426
PmlI CAC'GTG
Cuts at: 4591 4591
Size: 7426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5495 5527 7008 7040 5495
Size: 32 1481 32 5881
Fragments arranged by size:
5881 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3820 3918 3820
Size: 98 7328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7426
PsiI TTA'TAA
Cuts at: 4996 4996
Size: 7426
PspOMI G'GGCC_C
Cuts at: 3223 3223
Size: 7426
PstI C_TGCA'G
Cuts at: 830 4087 4778 830
Size: 3257 691 3478
Fragments arranged by size:
3478 3257 691
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7426
PvuII CAG'CTG
Cuts at: 2128 2314 2128
Size: 186 7240
SacI G_AGCT'C
Cuts at: 2039 2039
Size: 7426
SalI G'TCGA_C
Cuts at: 1936 4768 1936
Size: 2832 4594
SapI GCTCTTCn'nnn_
Cuts at: 2324 2324
Size: 7426
SbfI CC_TGCA'GG
Cuts at: 4778 4778
Size: 7426
ScaI AGT'ACT
Cuts at: 5674 7386 5674
Size: 1712 5714
SexAI A'CCwGG_T
Cuts at: 2223 2847 3483 2223
Size: 624 636 6166
Fragments arranged by size:
6166 636 624
SfcI C'TryA_G
Cuts at: 826 1045 2368 3568 4083 4774 5909 6590 6781
Size: 219 1323 1200 515 691 1135 681 191
Cuts at: 6781 826
Size: 1471
Fragments arranged by size:
1471 1323 1200 1135 691 681 515 219
191
SfoI GGC'GCC
Cuts at: 2270 4504 2270
Size: 2234 5192
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7426
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7426
SmaI CCC'GGG
Cuts at: 1872 2597 3538 4753 1872
Size: 725 941 1215 4545
Fragments arranged by size:
4545 1215 941 725
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2963 3041 4577 5530 6431 6693
Size: 197 926 1020 78 1536 953 901 262
Cuts at: 6693 820
Size: 1553
Fragments arranged by size:
1553 1536 1020 953 926 901 262 197
78
SnaBI TAC'GTA
Cuts at: 493 5217 493
Size: 4724 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7426
SphI G_CATG'C
Cuts at: 1132 4784 1132
Size: 3652 3774
SspI AAT'ATT
Cuts at: 5 52 5350 5
Size: 47 5298 2081
Fragments arranged by size:
5298 2081 47
StuI AGG'CCT
Cuts at: 1517 4664 1517
Size: 3147 4279
StyI C'CwwG_G
Cuts at: 513 1065 2442 2908 3153 3327 3558 3772 3800
Size: 552 1377 466 245 174 231 214 28
Cuts at: 3800 4526 4865 513
Size: 726 339 3074
Fragments arranged by size:
3074 1377 726 552 466 339 245 231
214 174 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 2701 3425 3922 4152 5453 5470
Size: 1255 615 506 724 497 230 1301 17
Cuts at: 5470 5623 5808 6253 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1301 1255 724 615 506 497 445
230 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2658 5672 7384
Size: 274 80 33 51 1443 681 3014 1712
Cuts at: 7384 96
Size: 138
Fragments arranged by size:
3014 1712 1443 681 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3590 3622 7386 7418 3590
Size: 32 3764 32 3598
Fragments arranged by size:
3764 3598 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7426
XbaI T'CTAG_A
Cuts at: 2983 4762 2983
Size: 1779 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2287 2765 3634 3994 4447 2053
Size: 234 478 869 360 453 5032
Fragments arranged by size:
5032 869 478 453 360 234
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7426
XmaI C'CCGG_G
Cuts at: 1870 2595 3536 4751 1870
Size: 725 941 1215 4545
Fragments arranged by size:
4545 1215 941 725
XmnI GAAnn'nnTTC
Cuts at: 5555 5555
Size: 7426
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3079 4207 5234 276
Size: 53 83 186 936 1545 1128 1027 2468
Fragments arranged by size:
2468 1545 1128 1027 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BstZ17I EcoRV FalI FseI NruI
NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI