Restriction map for
pFN21AA1525
(Circular) (Six-base) MAPSORT of: pFN21AA1525.seq Check: 6602 from: 1 to: 8101
pFN21AA1525 8101 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4000 4000
Size: 8101
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4997 5911 278
Size: 53 83 186 936 3461 914 2468
Fragments arranged by size:
3461 2468 936 914 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2436 3002 4449 4683 5444 1937
Size: 499 566 1447 234 761 4594
Fragments arranged by size:
4594 1447 761 566 499 234
Acc65I G'GTAC_C
Cuts at: 5422 5422
Size: 8101
AclI AA'CG_TT
Cuts at: 1563 4153 6228 6601 1563
Size: 2590 2075 373 3063
Fragments arranged by size:
3063 2590 2075 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2751 3906 4731 5265 6164 7548 7816 1632
Size: 1119 1155 825 534 899 1384 268 1917
Fragments arranged by size:
1917 1384 1155 1119 899 825 534 268
AflII C'TTAA_G
Cuts at: 820 1017 3601 820
Size: 197 2584 5320
Fragments arranged by size:
5320 2584 197
AflIII A'CryG_T
Cuts at: 1203 2733 7000 7977 1203
Size: 1530 4267 977 1327
Fragments arranged by size:
4267 1530 1327 977
AgeI A'CCGG_T
Cuts at: 5150 6932 5150
Size: 1782 6319
AhdI GACnn_n'nnGTC
Cuts at: 5287 6830 5287
Size: 1543 6558
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3717 1184
Size: 2533 5568
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3375 3407 3375
Size: 32 8069
AlwNI CAG_nnn'CTG
Cuts at: 2774 3994 4106 4574 4709 4738 5844 7416 2774
Size: 1220 112 468 135 29 1106 1572 3459
Fragments arranged by size:
3459 1572 1220 1106 468 135 112 29
ApaLI G'TGCA_C
Cuts at: 3257 6158 7314 3257
Size: 2901 1156 4044
Fragments arranged by size:
4044 2901 1156
ApoI r'AATT_y
Cuts at: 1493 2059 2681 2695 2872 3146 3290 3939 4065
Size: 566 622 14 177 274 144 649 126
Cuts at: 4065 4136 5410 5636 5788 7897 1493
Size: 71 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 649 622 566 274 226
177 152 144 126 71 14
AseI AT'TA_AT
Cuts at: 160 1032 6655 160
Size: 872 5623 1606
Fragments arranged by size:
5623 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 5426 1870
Size: 73 101 3382 4545
Fragments arranged by size:
4545 3382 101 73
BamHI G'GATC_C
Cuts at: 5431 5798 5431
Size: 367 7734
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4046 5422 6877 618
Size: 325 217 627 2259 1376 1455 1842
Fragments arranged by size:
2259 1842 1455 1376 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2826 3531 5308 5420 1391
Size: 1435 705 1777 112 4072
Fragments arranged by size:
4072 1777 1435 705 112
BbeI G_GCGC'C
Cuts at: 4050 4050
Size: 8101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5278 928
Size: 971 3379 3751
Fragments arranged by size:
3751 3379 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6292 6326 1903
Size: 34 4355 34 3678
Fragments arranged by size:
4355 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2883 5993 7203 2883
Size: 3110 1210 3781
Fragments arranged by size:
3781 3110 1210
BclI T'GATC_A
Cuts at: 1548 3879 1548
Size: 2331 5770
BfrBI ATG'CAT
Cuts at: 2018 2303 2018
Size: 285 7816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 2483 2837
Size: 107 122 71 631 605 691 120 354
Cuts at: 2837 6712 136
Size: 3875 1525
Fragments arranged by size:
3875 1525 691 631 605 354 122 120
107 71
BglII A'GATC_T
Cuts at: 4243 8096 4243
Size: 3853 4248
BlpI GC'TnA_GC
Cuts at: 5518 5518
Size: 8101
Bme1580I G_kGCm'C
Cuts at: 1790 3261 6162 7318 1790
Size: 1471 2901 1156 2573
Fragments arranged by size:
2901 2573 1471 1156
BmgBI CAC'GTC
Cuts at: 1318 3010 1318
Size: 1692 6409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4353 4439 4511
Size: 416 53 83 399 24 2936 86 72
Cuts at: 4511 5832 6790 442
Size: 1321 958 1753
Fragments arranged by size:
2936 1753 1321 958 416 399 86 83
72 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2288 3211 2288
Size: 923 7178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4399 6761 1239
Size: 3160 2362 2579
Fragments arranged by size:
3160 2579 2362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2623 3206 4637 6226 7091 7389 2623
Size: 583 1431 1589 865 298 3335
Fragments arranged by size:
3335 1589 1431 865 583 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6764 882
Size: 617 5265 2219
Fragments arranged by size:
5265 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5892 493
Size: 711 4688 2702
Fragments arranged by size:
4688 2702 711
BsaBI GATnn'nnATC
Cuts at: 8095 8095
Size: 8101
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4047 4994 5908 6290
Size: 53 83 186 936 2514 947 914 382
Cuts at: 6290 6925 275
Size: 635 1451
Fragments arranged by size:
2514 1451 947 936 914 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4831 5150 5264 6534 6932 7206 7353 7764 4831
Size: 319 114 1270 398 274 147 411 5168
Fragments arranged by size:
5168 1270 411 398 319 274 147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3043 3073 1178
Size: 30 165 30 1640 30 6206
Fragments arranged by size:
6206 1640 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 3627 3947 1186
Size: 1075 1366 320 5340
Fragments arranged by size:
5340 1366 1075 320
BseYI C'CCAG_C
Cuts at: 4306 4731 5106 7304 7800 4306
Size: 425 375 2198 496 4607
Fragments arranged by size:
4607 2198 496 425 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2835 4034 4455 1163
Size: 573 1099 1199 421 4809
Fragments arranged by size:
4809 1199 1099 573 421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5551 6312 7340 1540
Size: 275 186 3550 761 1028 2301
Fragments arranged by size:
3550 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2826 3261 3531 5308 6162 6247 7318 2826
Size: 435 270 1777 854 85 1071 3609
Fragments arranged by size:
3609 1777 1071 854 435 270 85
BsmI GAATG_Cn'
Cuts at: 5612 5705 5612
Size: 93 8008
BsmBI CGTCTCn'nnnn_
Cuts at: 3959 5001 3959
Size: 1042 7059
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2826 3261 3531 5308 5420 6162 6247
Size: 399 1036 435 270 1777 112 742 85
Cuts at: 6247 7318 1391
Size: 1071 2174
Fragments arranged by size:
2174 1777 1071 1036 742 435 399 270
112 85
BspEI T'CCGG_A
Cuts at: 4831 7764 4831
Size: 2933 5168
BspHI T'CATG_A
Cuts at: 3303 5990 7720 3303
Size: 2687 1730 3684
Fragments arranged by size:
3684 2687 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4000 5456 844
Size: 3156 1456 3489
Fragments arranged by size:
3489 3156 1456
BsrBI CCG'CTC
Cuts at: 1394 1823 5988 1394
Size: 429 4165 3507
Fragments arranged by size:
4165 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2384 3180 3618 6596 6770 65
Size: 1183 1136 796 438 2978 174 1396
Fragments arranged by size:
2978 1396 1183 1136 796 438 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5150 6745 6932 1700
Size: 110 3340 1595 187 2869
Fragments arranged by size:
3340 2869 1595 187 110
BsrGI T'GTAC_A
Cuts at: 96 4935 96
Size: 4839 3262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8101
BssSI C'ACGA_G
Cuts at: 6161 7173 6161
Size: 1012 7089
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3994 4106 794
Size: 3200 112 4789
Fragments arranged by size:
4789 3200 112
BstBI TT'CG_AA
Cuts at: 2869 6905 2869
Size: 4036 4065
BstEII G'GTnAC_C
Cuts at: 1184 5358 1184
Size: 4174 3927
BstYI r'GATC_y
Cuts at: 1972 3537 3790 4243 5261 5431 5798 6183 6200
Size: 1565 253 453 1018 170 367 385 17
Cuts at: 6200 7652 7738 7750 8096 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1565 1452 1018 453 385 367 346
253 170 86 17 12
BstZ17I GTA'TAC
Cuts at: 3003 3003
Size: 8101
BtgI C'CryG_G
Cuts at: 513 1065 2004 2146 3114 5299 513
Size: 552 939 142 968 2185 3315
Fragments arranged by size:
3315 2185 968 939 552 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4609 487
Size: 683 56 3383 3979
Fragments arranged by size:
3979 3383 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 3471 4491 5617 6411 6431 2510
Size: 961 1020 1126 794 20 4180
Fragments arranged by size:
4180 1126 1020 961 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8066
DraI TTT'AAA
Cuts at: 2506 5405 2506
Size: 2899 5202
DraIII CAC_nnn'GTG
Cuts at: 4961 4961
Size: 8101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4359 7108 809
Size: 507 3043 2749 1802
Fragments arranged by size:
3043 2749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2149 5548 6437
Size: 54 1425 50 275 18 319 3399 889
Cuts at: 6437 8066 8
Size: 1629 43
Fragments arranged by size:
3399 1629 1425 889 319 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5548 1537
Size: 275 3736 4090
Fragments arranged by size:
4090 3736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2814 3519 3734 5250 6031 1353
Size: 1461 705 215 1516 781 3423
Fragments arranged by size:
3423 1516 1461 781 705 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6688 7062 7208 387
Size: 1064 138 5099 374 146 1280
Fragments arranged by size:
5099 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2824 3529 5306 2824
Size: 705 1777 5619
Fragments arranged by size:
5619 1777 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2751 3906 4399 4731 5265 6164 6761
Size: 393 1119 1155 493 332 534 899 597
Cuts at: 6761 7548 7816 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1155 1119 899 787 597 534 493
393 332 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3284 4908 3284
Size: 1624 6477
EcoO109I rG'GnC_Cy
Cuts at: 2088 3511 4020 4724 2088
Size: 1423 509 704 5465
Fragments arranged by size:
5465 1423 704 509
EcoRI G'AATT_C
Cuts at: 5410 5788 5410
Size: 378 7723
EcoRV GAT'ATC
Cuts at: 2659 3178 3391 2659
Size: 519 213 7369
Fragments arranged by size:
7369 519 213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3410 3936 5392 3410
Size: 526 1456 6119
Fragments arranged by size:
6119 1456 526
FspI TGC'GCA
Cuts at: 6607 6607
Size: 8101
HaeII r_GCGC'y
Cuts at: 1678 4050 7248 1678
Size: 2372 3198 2531
Fragments arranged by size:
3198 2531 2372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 3220 3252 3619
Size: 32 135 32 740 32 876 32 367
Cuts at: 3619 3651 4235 4267 5014 5046 5273 5305 6742
Size: 32 584 32 747 32 227 32 1437
Cuts at: 6742 6774 6816 6848 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1437 876 747 740 584 367 227
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5445 5691 669
Size: 988 281 3507 246 3079
Fragments arranged by size:
3507 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2787 3267 748
Size: 2039 480 5582
Fragments arranged by size:
5582 2039 480
HpaI GTT'AAC
Cuts at: 5691 5691
Size: 8101
KasI G'GCGC_C
Cuts at: 4046 4046
Size: 8101
KpnI G_GTAC'C
Cuts at: 5426 5426
Size: 8101
MfeI C'AATT_G
Cuts at: 5700 5700
Size: 8101
MluI A'CGCG_T
Cuts at: 7977 7977
Size: 8101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2204 7215 7399 1305
Size: 899 5011 184 2007
Fragments arranged by size:
5011 2007 899 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2151 10
Size: 54 1425 343 319 5960
Fragments arranged by size:
5960 1425 343 319 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 3240 4628 5085 6059 6418 6577
Size: 648 985 1089 1388 457 974 359 159
Cuts at: 6577 518
Size: 2042
Fragments arranged by size:
2042 1388 1089 985 974 648 457 359
159
MspA1I CmG'CkG
Cuts at: 1606 2567 5110 5517 6194 7342 7587 1606
Size: 961 2543 407 677 1148 245 2120
Fragments arranged by size:
2543 2120 1148 961 677 407 245
NarI GG'CG_CC
Cuts at: 4047 4047
Size: 8101
NcoI C'CATG_G
Cuts at: 513 1065 2004 2146 3114 5299 513
Size: 552 939 142 968 2185 3315
Fragments arranged by size:
3315 2185 968 939 552 142
NdeI CA'TA_TG
Cuts at: 387 3241 387
Size: 2854 5247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8101
NotI GC'GGCC_GC
Cuts at: 5548 5548
Size: 8101
NsiI A_TGCA'T
Cuts at: 2020 2305 2020
Size: 285 7816
NspI r_CATG'y
Cuts at: 1132 1229 2737 3157 5238 5459 7004 1132
Size: 97 1508 420 2081 221 1545 2229
Fragments arranged by size:
2229 2081 1545 1508 420 221 97
PasI CC'CwG_GG
Cuts at: 1348 5293 1348
Size: 3945 4156
PciI A'CATG_T
Cuts at: 2733 7000 2733
Size: 4267 3834
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2534 1108
Size: 1426 6675
PmeI GTTT'AAAC
Cuts at: 5405 5405
Size: 8101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6170 6202 7683 7715 6170
Size: 32 1481 32 6556
Fragments arranged by size:
6556 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2088 3511 2088
Size: 1423 6678
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8101
PsiI TTA'TAA
Cuts at: 5671 5671
Size: 8101
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3564 3596 3564
Size: 32 8069
PstI C_TGCA'G
Cuts at: 830 3990 4114 4228 4498 5182 5453 830
Size: 3160 124 114 270 684 271 3478
Fragments arranged by size:
3478 3160 684 271 270 124 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8101
PvuII CAG'CTG
Cuts at: 2567 2567
Size: 8101
SacI G_AGCT'C
Cuts at: 2826 3531 5308 2826
Size: 705 1777 5619
Fragments arranged by size:
5619 1777 705
SalI G'TCGA_C
Cuts at: 1936 5443 1936
Size: 3507 4594
SapI GCTCTTCn'nnn_
Cuts at: 2814 3519 2814
Size: 705 7396
SbfI CC_TGCA'GG
Cuts at: 5453 5453
Size: 8101
ScaI AGT'ACT
Cuts at: 3634 6349 8061 3634
Size: 2715 1712 3674
Fragments arranged by size:
3674 2715 1712
SexAI A'CCwGG_T
Cuts at: 4984 4984
Size: 8101
SfcI C'TryA_G
Cuts at: 826 1045 3034 3371 3986 4110 4224 4494 4680
Size: 219 1989 337 615 124 114 270 186
Cuts at: 4680 5178 5449 6584 7265 7456 826
Size: 498 271 1135 681 191 1471
Fragments arranged by size:
1989 1471 1135 681 615 498 337 271
270 219 191 186 124 114
SfoI GGC'GCC
Cuts at: 4048 4048
Size: 8101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8101
SmaI CCC'GGG
Cuts at: 1872 5428 1872
Size: 3556 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2638 3221 3601 4652 6205 7106
Size: 197 926 695 583 380 1051 1553 901
Cuts at: 7106 7368 820
Size: 262 1553
Fragments arranged by size:
1553 1553 1051 926 901 695 583 380
262 197
SnaBI TAC'GTA
Cuts at: 493 5892 493
Size: 5399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8101
SphI G_CATG'C
Cuts at: 1132 5459 1132
Size: 4327 3774
SspI AAT'ATT
Cuts at: 5 52 2445 6025 5
Size: 47 2393 3580 2081
Fragments arranged by size:
3580 2393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8101
StyI C'CwwG_G
Cuts at: 513 1065 2004 2146 3114 5299 5540 513
Size: 552 939 142 968 2185 241 3074
Fragments arranged by size:
3074 2185 968 939 552 241 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2268 2931 3244 4622 6128 6145
Size: 1255 417 271 663 313 1378 1506 17
Cuts at: 6145 6298 6483 6928 325
Size: 153 185 445 1498
Fragments arranged by size:
1506 1498 1378 1255 663 445 417 313
271 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 2426 3632
Size: 274 80 33 51 1443 438 11 1206
Cuts at: 3632 4214 4935 6347 8059 96
Size: 582 721 1412 1712 138
Fragments arranged by size:
1712 1443 1412 1206 721 582 438 274
138 80 51 33 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3043 3075 8061 8093 3043
Size: 32 4986 32 3051
Fragments arranged by size:
4986 3051 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3974 1316
Size: 2658 5443
XbaI T'CTAG_A
Cuts at: 5437 5437
Size: 8101
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8101
XmaI C'CCGG_G
Cuts at: 1870 5426 1870
Size: 3556 4545
XmnI GAAnn'nnTTC
Cuts at: 6230 6230
Size: 8101
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4995 5909 276
Size: 53 83 186 936 3461 914 2468
Fragments arranged by size:
3461 2468 936 914 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BstXI Bsu36I FseI FspAI NaeI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI RsrII SacII SanDI SfiI
SrfI SwaI XcmI