Restriction map for
pFN21AA1512
(Circular) (Six-base) MAPSORT of: pFN21AA1512.seq Check: 2896 from: 1 to: 10654
pFN21AA1512 10654 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3385 5758 7573 3385
Size: 2373 1815 6466
Fragments arranged by size:
6466 2373 1815
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8464 278
Size: 53 83 186 936 6928 2468
Fragments arranged by size:
6928 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2994 7997 1937
Size: 1057 5003 4594
Fragments arranged by size:
5003 4594 1057
Acc65I G'GTAC_C
Cuts at: 4314 7975 4314
Size: 3661 6993
AclI AA'CG_TT
Cuts at: 1563 8781 9154 1563
Size: 7218 373 3063
Fragments arranged by size:
7218 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3145 5348 7478 8717 10101 10369 1632
Size: 1513 2203 2130 1239 1384 268 1917
Fragments arranged by size:
2203 2130 1917 1513 1384 1239 268
AfeI AGC'GCT
Cuts at: 2450 4862 2450
Size: 2412 8242
AflII C'TTAA_G
Cuts at: 820 1017 7661 820
Size: 197 6644 3813
Fragments arranged by size:
6644 3813 197
AflIII A'CryG_T
Cuts at: 1203 3615 9553 10530 1203
Size: 2412 5938 977 1327
Fragments arranged by size:
5938 2412 1327 977
AgeI A'CCGG_T
Cuts at: 9485 9485
Size: 10654
AhdI GACnn_n'nnGTC
Cuts at: 2641 9383 2641
Size: 6742 3912
AleI CACnn'nnGTG
Cuts at: 1166 2049 2118 4436 6168 1166
Size: 883 69 2318 1732 5652
Fragments arranged by size:
5652 2318 1732 883 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10654
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 4568 4600 3740
Size: 32 796 32 9794
Fragments arranged by size:
9794 796 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3478 4492 4561 4768 5170 5377 5800 6157 6229
Size: 1014 69 207 402 207 423 357 72
Cuts at: 6229 6467 6470 6727 7138 7171 7594 7750 8397
Size: 238 3 257 411 33 423 156 647
Cuts at: 8397 9969 3478
Size: 1572 4163
Fragments arranged by size:
4163 1572 1014 647 423 423 411 402
357 257 238 207 207 156 72 69
33 3
ApaI G_GGCC'C
Cuts at: 5458 5542 6802 6973 7940 5458
Size: 84 1260 171 967 8172
Fragments arranged by size:
8172 1260 967 171 84
ApaLI G'TGCA_C
Cuts at: 2201 4990 7479 8711 9867 2201
Size: 2789 2489 1232 1156 2988
Fragments arranged by size:
2988 2789 2489 1232 1156
ApoI r'AATT_y
Cuts at: 1493 7963 8189 8341 10450 1493
Size: 6470 226 152 2109 1697
Fragments arranged by size:
6470 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9208 160
Size: 872 8176 1606
Fragments arranged by size:
8176 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10654
AvaI C'yCGr_G
Cuts at: 1870 1943 3345 5490 5535 7923 7979 1870
Size: 73 1402 2145 45 2388 56 4545
Fragments arranged by size:
4545 2388 2145 1402 73 56 45
AvrII C'CTAG_G
Cuts at: 3154 3154
Size: 10654
BamHI G'GATC_C
Cuts at: 3937 4254 6831 7984 8351 3937
Size: 317 2577 1153 367 6240
Fragments arranged by size:
6240 2577 1153 367 317
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2239 2780 3200 3898
Size: 325 217 627 264 188 541 420 698
Cuts at: 3898 4314 6099 6584 7975 9430 618
Size: 416 1785 485 1391 1455 1842
Fragments arranged by size:
1842 1785 1455 1391 698 627 541 485
420 416 325 264 217 188
BanII G_rGCy'C
Cuts at: 1391 3126 3418 4022 4038 4394 4479 5001 5458
Size: 1735 292 604 16 356 85 522 457
Cuts at: 5458 5542 6594 6802 6846 6973 7152 7530 7701
Size: 84 1052 208 44 127 179 378 171
Cuts at: 7701 7940 7973 1391
Size: 239 33 4072
Fragments arranged by size:
4072 1735 1052 604 522 457 378 356
292 239 208 179 171 127 85 84
44 33 16
BbeI G_GCGC'C
Cuts at: 3204 6103 3204
Size: 2899 7755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3658 5442 7508 928
Size: 971 1759 1784 2066 4074
Fragments arranged by size:
4074 2066 1784 1759 971
BbvCI CC'TCA_GC
Cuts at: 4686 6204 6460 7404 4686
Size: 1518 256 944 7936
Fragments arranged by size:
7936 1518 944 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5828 5862 6266 6300 8845 8879 1903
Size: 34 3891 34 404 34 2545 34 3678
Fragments arranged by size:
3891 3678 2545 404 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4331 8546 9756 4331
Size: 4215 1210 5229
Fragments arranged by size:
5229 4215 1210
BclI T'GATC_A
Cuts at: 1548 2652 4875 6042 1548
Size: 1104 2223 1167 6160
Fragments arranged by size:
6160 2223 1167 1104
BfrBI ATG'CAT
Cuts at: 3753 3753
Size: 10654
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2822 4709 5717
Size: 107 122 71 631 605 1150 1887 1008
Cuts at: 5717 6152 6254 7064 7421 7445 9265 136
Size: 435 102 810 357 24 1820 1525
Fragments arranged by size:
1887 1820 1525 1150 1008 810 631 605
435 357 122 107 102 71 24
BglII A'GATC_T
Cuts at: 3537 10649 3537
Size: 7112 3542
BlpI GC'TnA_GC
Cuts at: 4830 5411 5420 5588 5918 6053 6216 7146 7361
Size: 581 9 168 330 135 163 930 215
Cuts at: 7361 7623 8071 4830
Size: 262 448 7413
Fragments arranged by size:
7413 930 581 448 330 262 215 168
163 135 9
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2783 4994 5458 5542 6136 6508 6587
Size: 415 578 2211 464 84 594 372 79
Cuts at: 6587 6802 6973 7483 7918 7940 8715 9871 1790
Size: 215 171 510 435 22 775 1156 2573
Fragments arranged by size:
2573 2211 1156 775 594 578 510 464
435 415 372 215 171 84 79 22
BmgBI CAC'GTC
Cuts at: 1318 4783 1318
Size: 3465 7189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2112 2516 3670
Size: 416 53 83 399 24 695 404 1154
Cuts at: 3670 3731 4326 4408 6253 8385 9343 442
Size: 61 595 82 1845 2132 958 1753
Fragments arranged by size:
2132 1845 1753 1154 958 695 595 416
404 399 83 82 61 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2408 2963 3647 3761 3944 4268 4790
Size: 833 336 555 684 114 183 324 522
Cuts at: 4790 4916 4949 5000 5042 5252 5285 5336 5399
Size: 126 33 51 42 210 33 51 63
Cuts at: 5399 5537 5600 5621 5705 5822 6329 6461 6467
Size: 138 63 21 84 117 507 132 6
Cuts at: 6467 6530 6572 6656 6749 6803 6824 6830 6860
Size: 63 42 84 93 54 21 6 30
Cuts at: 6860 7046 7193 7226 7247 7361 7514 7529 7616
Size: 186 147 33 21 114 153 15 87
Cuts at: 7616 7637 9314 1239
Size: 21 1677 2579
Fragments arranged by size:
2579 1677 833 684 555 522 507 336
324 210 186 183 153 147 138 132
126 117 114 114 93 87 84 84
63 63 63 54 51 51 42 42
33 33 33 30 21 21 21 21
15 6 6
Bpu10I CC'TnA_GC
Cuts at: 4686 6204 6460 7404 4686
Size: 1518 256 944 7936
Fragments arranged by size:
7936 1518 944 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2807 4568 4985 5346 5375 6629 6977 7085 7562
Size: 1761 417 361 29 1254 348 108 477
Cuts at: 7562 8779 9644 9942 2807
Size: 1217 865 298 3519
Fragments arranged by size:
3519 1761 1254 1217 865 477 417 361
348 298 108 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2306 3944 6809 9317 882
Size: 617 807 1638 2865 2508 2219
Fragments arranged by size:
2865 2508 2219 1638 807 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 3370 8445 493
Size: 711 1059 1107 5075 2702
Fragments arranged by size:
5075 2702 1107 1059 711
BsaBI GATnn'nnATC
Cuts at: 4259 10648 4259
Size: 6389 4265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 3087 3201 3774
Size: 53 83 186 936 723 831 114 573
Cuts at: 3774 4464 6100 7929 8461 8843 9478 275
Size: 690 1636 1829 532 382 635 1451
Fragments arranged by size:
1829 1636 1451 936 831 723 690 635
573 532 382 186 114 83 53
BsaWI w'CCGG_w
Cuts at: 2657 4257 6870 9087 9485 9759 9906 10317 2657
Size: 1600 2613 2217 398 274 147 411 2994
Fragments arranged by size:
2994 2613 2217 1600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4973 5003 6020 6050 1178
Size: 30 165 30 3570 30 1017 30 5782
Fragments arranged by size:
5782 3570 1017 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2096 2851 3134 3263 3314 3650 3758
Size: 907 3 755 283 129 51 336 108
Cuts at: 3758 4703 4706 4787 4871 4952 4955 5204 5396
Size: 945 3 81 84 81 3 249 192
Cuts at: 5396 5513 5525 5597 5618 5702 5957 6041 6125
Size: 117 12 72 21 84 255 84 84
Cuts at: 6125 6209 6212 6280 6317 6326 6368 6545 6548
Size: 84 3 68 37 9 42 177 3
Cuts at: 6548 6632 6686 6800 6827 6908 7121 7142 7223
Size: 84 54 114 27 81 213 21 81
Cuts at: 7223 7391 7421 7466 7559 7730 7814 7886 1186
Size: 168 30 45 93 171 84 72 3954
Fragments arranged by size:
3954 945 907 755 336 283 255 249
213 192 177 171 168 129 117 114
108 93 84 84 84 84 84 84
84 81 81 81 81 72 72 68
54 51 45 42 37 30 27 21
21 12 9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3527 4095 4387 4451 4564 5662 5758 7036 7356
Size: 568 292 64 113 1098 96 1278 320
Cuts at: 7356 7848 9857 10353 3527
Size: 492 2009 496 3828
Fragments arranged by size:
3828 2009 1278 1098 568 496 492 320
292 113 96 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 3194 4085 4910 5580 5904 6170
Size: 573 681 777 891 825 670 324 266
Cuts at: 6170 6762 7368 7607 1163
Size: 592 606 239 4210
Fragments arranged by size:
4210 891 825 777 681 670 606 592
573 324 266 239
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4749 8104 8865 9893 1540
Size: 275 186 2748 3355 761 1028 2301
Fragments arranged by size:
3355 2748 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 3126 4479 4994 5001 6386 6594 6846 7152
Size: 921 1353 515 7 1385 208 252 306
Cuts at: 7152 7483 7530 7701 8715 8800 9871 2205
Size: 331 47 171 1014 85 1071 2988
Fragments arranged by size:
2988 1385 1353 1071 1014 921 515 331
306 252 208 171 85 47 7
BsmI GAATG_Cn'
Cuts at: 2886 3755 5261 7300 8165 8258 2886
Size: 869 1506 2039 865 93 5282
Fragments arranged by size:
5282 2039 1506 869 865 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2727 4769 6632 2727
Size: 2042 1863 6749
Fragments arranged by size:
6749 2042 1863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2783 3126 3418 4022 4038 4394
Size: 399 415 578 343 292 604 16 356
Cuts at: 4394 4479 4994 5001 5458 5542 6136 6386 6508
Size: 85 515 7 457 84 594 250 122
Cuts at: 6508 6587 6594 6802 6846 6973 7152 7483 7530
Size: 79 7 208 44 127 179 331 47
Cuts at: 7530 7701 7918 7940 7973 8715 8800 9871 1391
Size: 171 217 22 33 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 594 578 515 457
415 399 356 343 331 292 250 217
208 179 171 127 122 85 85 84
79 47 44 33 22 16 7 7
BspEI T'CCGG_A
Cuts at: 4257 10317 4257
Size: 6060 4594
BspHI T'CATG_A
Cuts at: 8543 10273 8543
Size: 1730 8924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 3385 3696 4143 5187 5758 6672 7573
Size: 1604 937 311 447 1044 571 914 901
Cuts at: 7573 8009 844
Size: 436 3489
Fragments arranged by size:
3489 1604 1044 937 914 901 571 447
436 311
BsrBI CCG'CTC
Cuts at: 1394 1823 2568 4924 5299 5527 5596 6451 7365
Size: 429 745 2356 375 228 69 855 914
Cuts at: 7365 7876 8541 1394
Size: 511 665 3507
Fragments arranged by size:
3507 2356 914 855 745 665 511 429
375 228 69
BsrDI GCAATG_nn'
Cuts at: 65 1248 4243 6982 9149 9323 65
Size: 1183 2995 2739 2167 174 1396
Fragments arranged by size:
2995 2739 2167 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2672 3438 4968 7245 9298 9485 1700
Size: 110 862 766 1530 2277 2053 187 2869
Fragments arranged by size:
2869 2277 2053 1530 862 766 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10654
BssHII G'CGCG_C
Cuts at: 1925 5205 1925
Size: 3280 7374
BssSI C'ACGA_G
Cuts at: 5705 6191 6614 7154 7853 8714 9726 5705
Size: 486 423 540 699 861 1012 6633
Fragments arranged by size:
6633 1012 861 699 540 486 423
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 3037 5788 6157 6229 6470 7519 794
Size: 1646 597 2751 369 72 241 1049 3929
Fragments arranged by size:
3929 2751 1646 1049 597 369 241 72
BstBI TT'CG_AA
Cuts at: 3558 9458 3558
Size: 5900 4754
BstEII G'GTnAC_C
Cuts at: 1184 5174 5321 7010 1184
Size: 3990 147 1689 4828
Fragments arranged by size:
4828 3990 1689 147
BstXI CCAn_nnnn'nTGG
Cuts at: 2972 2972
Size: 10654
BstYI r'GATC_y
Cuts at: 1972 3003 3537 3937 4254 4260 6768 6831 7984
Size: 1031 534 400 317 6 2508 63 1153
Cuts at: 7984 8351 8736 8753 10205 10291 10303 10649 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2508 1977 1452 1153 1031 534 400 385
367 346 317 86 63 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 2846 6908 2846
Size: 4062 6592
BtgI C'CryG_G
Cuts at: 513 1065 2047 2213 2307 2817 2919 3096 3144
Size: 552 982 166 94 510 102 177 48
Cuts at: 3144 3954 4528 5622 6058 6574 7567 513
Size: 810 574 1094 436 516 993 3600
Fragments arranged by size:
3600 1094 993 982 810 574 552 516
510 436 177 166 102 94 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3112 3508 487
Size: 683 56 1886 396 7633
Fragments arranged by size:
7633 1886 683 396 56
BtsI GCAGTG_nn'
Cuts at: 2124 3319 5723 6332 8170 8964 8984 2124
Size: 1195 2404 609 1838 794 20 3794
Fragments arranged by size:
3794 2404 1838 1195 794 609 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10619
DraI TTT'AAA
Cuts at: 7958 7958
Size: 10654
DraIII CAC_nnn'GTG
Cuts at: 6148 6148
Size: 10654
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 9661 809
Size: 507 4025 4320 1802
Fragments arranged by size:
4320 4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 3420 4426
Size: 54 1425 50 275 18 567 1023 1006
Cuts at: 4426 4494 4531 4746 4959 5034 6072 6610 7437
Size: 68 37 215 213 75 1038 538 827
Cuts at: 7437 7446 7830 8101 8990 10619 8
Size: 9 384 271 889 1629 43
Fragments arranged by size:
1629 1425 1038 1023 1006 889 827 567
538 384 275 271 215 213 75 68
54 50 43 37 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4746 8101 1537
Size: 275 2934 3355 4090
Fragments arranged by size:
4090 3355 2934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2492 4467 5085 5502 5807 6084 6599 6707
Size: 1139 1975 618 417 305 277 515 108
Cuts at: 6707 6755 8584 1353
Size: 48 1829 3423
Fragments arranged by size:
3423 1975 1829 1139 618 515 417 305
277 108 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2162 2615 3272 5510 5672 5756
Size: 1064 138 573 453 657 2238 162 84
Cuts at: 5756 5768 5978 7361 7820 7904 9241 9615 9761
Size: 12 210 1383 459 84 1337 374 146
Cuts at: 9761 387
Size: 1280
Fragments arranged by size:
2238 1383 1337 1280 1064 657 573 459
453 374 210 162 146 138 84 84
12
EcoICRI GAG'CTC
Cuts at: 3124 4477 4999 6592 6844 7150 7528 7699 3124
Size: 1353 522 1593 252 306 378 171 6079
Fragments arranged by size:
6079 1593 1353 522 378 306 252 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2408 2963 3145 3647 3761 3944
Size: 393 440 336 555 182 502 114 183
Cuts at: 3944 4268 4790 4916 4949 5000 5042 5252 5285
Size: 324 522 126 33 51 42 210 33
Cuts at: 5285 5336 5348 5399 5537 5600 5621 5705 5822
Size: 51 12 51 138 63 21 84 117
Cuts at: 5822 6329 6461 6467 6530 6572 6656 6749 6803
Size: 507 132 6 63 42 84 93 54
Cuts at: 6803 6824 6830 6860 7046 7193 7226 7247 7361
Size: 21 6 30 186 147 33 21 114
Cuts at: 7361 7478 7514 7529 7616 7637 8717 9314 10101
Size: 117 36 15 87 21 1080 597 787
Cuts at: 10101 10369 1239
Size: 268 1524
Fragments arranged by size:
1524 1080 787 597 555 522 507 502
440 393 336 324 268 210 186 183
182 147 138 132 126 117 117 114
114 93 87 84 84 63 63 54
51 51 51 42 42 36 33 33
33 30 21 21 21 21 15 12
6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2417 3743 4496 5267 6023 6668 7229 7544
Size: 243 1326 753 771 756 645 561 315
Cuts at: 7544 2174
Size: 5284
Fragments arranged by size:
5284 1326 771 756 753 645 561 315
243
EcoO109I rG'GnC_Cy
Cuts at: 3319 4243 5167 5248 5454 6067 6798 6799 7657
Size: 924 924 81 206 613 731 1 858
Cuts at: 7657 7747 7937 3319
Size: 90 190 6036
Fragments arranged by size:
6036 924 924 858 731 613 206 190
90 81 1
EcoRI G'AATT_C
Cuts at: 7963 8341 7963
Size: 378 10276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4459 4459
Size: 10654
FspI TGC'GCA
Cuts at: 2627 5285 5825 7673 9160 2627
Size: 2658 540 1848 1487 4121
Fragments arranged by size:
4121 2658 1848 1487 540
HaeII r_GCGC'y
Cuts at: 1678 2452 2566 3204 4492 4561 4739 4762 4864
Size: 774 114 638 1288 69 178 23 102
Cuts at: 4864 5699 6103 6749 6986 7180 7288 7687 7792
Size: 835 404 646 237 194 108 399 105
Cuts at: 7792 9801 1678
Size: 2009 2531
Fragments arranged by size:
2531 2009 1288 835 774 646 638 404
399 237 194 178 114 108 105 102
69 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2627 2659 2846
Size: 32 135 32 692 32 331 32 187
Cuts at: 2846 2878 9295 9327 9369 9401 1373
Size: 32 6417 32 42 32 2626
Fragments arranged by size:
6417 2626 692 331 187 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 7998 8244 669
Size: 988 281 748 5312 246 3079
Fragments arranged by size:
5312 3079 988 748 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10654
HpaI GTT'AAC
Cuts at: 2686 8244 2686
Size: 5558 5096
KasI G'GCGC_C
Cuts at: 3200 6099 3200
Size: 2899 7755
KpnI G_GTAC'C
Cuts at: 4318 7979 4318
Size: 3661 6993
MfeI C'AATT_G
Cuts at: 8253 8253
Size: 10654
MluI A'CGCG_T
Cuts at: 10530 10530
Size: 10654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 6372 9768 9952 1305
Size: 957 4110 3396 184 2007
Fragments arranged by size:
4110 3396 2007 957 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 3422 4428 4496 5036
Size: 54 1425 343 567 1023 1006 68 540
Cuts at: 5036 7832 10
Size: 2796 2832
Fragments arranged by size:
2832 2796 1425 1023 1006 567 540 343
68 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2118 2129 2399 2589 3095 3620
Size: 648 883 69 11 270 190 506 525
Cuts at: 3620 4355 4436 5831 6168 8612 8971 9130 518
Size: 735 81 1395 337 2444 359 159 2042
Fragments arranged by size:
2444 2042 1395 883 735 648 525 506
359 337 270 190 159 81 69 11
MspA1I CmG'CkG
Cuts at: 1606 2460 2921 3244 3565 4099 4164 4228 4312
Size: 854 461 323 321 534 65 64 84
Cuts at: 4312 4666 4822 4894 5377 5419 5624 5785 5953
Size: 354 156 72 483 42 205 161 168
Cuts at: 5953 5959 6052 6226 6467 6490 6544 6838 7036
Size: 6 93 174 241 23 54 294 198
Cuts at: 7036 7135 7145 7360 8070 8747 9895 10140 1606
Size: 99 10 215 710 677 1148 245 2120
Fragments arranged by size:
2120 1148 854 710 677 534 483 461
354 323 321 294 245 241 215 205
198 174 168 161 156 99 93 84
72 65 64 54 42 23 10 6
NaeI GCC'GGC
Cuts at: 4970 7247 4970
Size: 2277 8377
NarI GG'CG_CC
Cuts at: 3201 6100 3201
Size: 2899 7755
NcoI C'CATG_G
Cuts at: 513 1065 2213 2817 3144 513
Size: 552 1148 604 327 8023
Fragments arranged by size:
8023 1148 604 552 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10654
NgoMIV G'CCGG_C
Cuts at: 4968 7245 4968
Size: 2277 8377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10654
NotI GC'GGCC_GC
Cuts at: 8101 8101
Size: 10654
NsiI A_TGCA'T
Cuts at: 3755 3755
Size: 10654
NspI r_CATG'y
Cuts at: 1132 1229 2623 3040 3619 3757 7744 8012 9557
Size: 97 1394 417 579 138 3987 268 1545
Cuts at: 9557 1132
Size: 2229
Fragments arranged by size:
3987 2229 1545 1394 579 417 268 138
97
PasI CC'CwG_GG
Cuts at: 1348 2728 4193 5170 7933 1348
Size: 1380 1465 977 2763 4069
Fragments arranged by size:
4069 2763 1465 1380 977
PciI A'CATG_T
Cuts at: 3615 9553 3615
Size: 5938 4716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2761 3145 3388 3478 4492 4596 4768 6085
Size: 1653 384 243 90 1014 104 172 1317
Cuts at: 6085 6727 1108
Size: 642 5035
Fragments arranged by size:
5035 1653 1317 1014 642 384 243 172
104 90
PfoI T'CCnGG_A
Cuts at: 2940 3738 4245 4341 4467 6720 2940
Size: 798 507 96 126 2253 6874
Fragments arranged by size:
6874 2253 798 507 126 96
PmeI GTTT'AAAC
Cuts at: 7958 7958
Size: 10654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2408 2440 8723 8755 10236 10268 2408
Size: 32 6283 32 1481 32 2794
Fragments arranged by size:
6283 2794 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3319 4243 5248 7747 3319
Size: 924 1005 2499 6226
Fragments arranged by size:
6226 2499 1005 924
PshAI GACnn'nnGTC
Cuts at: 1613 3956 4781 1613
Size: 2343 825 7486
Fragments arranged by size:
7486 2343 825
PsiI TTA'TAA
Cuts at: 8224 8224
Size: 10654
PspOMI G'GGCC_C
Cuts at: 5454 5538 6798 6969 7936 5454
Size: 84 1260 171 967 8172
Fragments arranged by size:
8172 1260 967 171 84
PstI C_TGCA'G
Cuts at: 830 2358 3033 3080 3693 4518 4821 4827 5184
Size: 1528 675 47 613 825 303 6 357
Cuts at: 5184 5487 5784 5952 6225 6345 6669 6945 7035
Size: 303 297 168 273 120 324 276 90
Cuts at: 7035 7767 8006 830
Size: 732 239 3478
Fragments arranged by size:
3478 1528 825 732 675 613 357 324
303 303 297 276 273 239 168 120
90 47 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10654
PvuII CAG'CTG
Cuts at: 3565 4099 4228 4822 4894 5377 5419 5959 6052
Size: 534 129 594 72 483 42 540 93
Cuts at: 6052 6467 6838 7036 7145 7360 3565
Size: 415 371 198 109 215 6859
Fragments arranged by size:
6859 594 540 534 483 415 371 215
198 129 109 93 72 42
SacI G_AGCT'C
Cuts at: 3126 4479 5001 6594 6846 7152 7530 7701 3126
Size: 1353 522 1593 252 306 378 171 6079
Fragments arranged by size:
6079 1593 1353 522 378 306 252 171
SacII CC_GC'GG
Cuts at: 2922 5625 2922
Size: 2703 7951
SalI G'TCGA_C
Cuts at: 1936 7996 1936
Size: 6060 4594
SapI GCTCTTCn'nnn_
Cuts at: 4467 6599 6707 4467
Size: 2132 108 8414
Fragments arranged by size:
8414 2132 108
SbfI CC_TGCA'GG
Cuts at: 5184 6669 6945 8006 5184
Size: 1485 276 1061 7832
Fragments arranged by size:
7832 1485 1061 276
ScaI AGT'ACT
Cuts at: 2708 8902 10614 2708
Size: 6194 1712 2748
Fragments arranged by size:
6194 2748 1712
SexAI A'CCwGG_T
Cuts at: 6693 7749 6693
Size: 1056 9598
SfcI C'TryA_G
Cuts at: 826 1045 2354 3029 3076 3689 4514 4817 4823
Size: 219 1309 675 47 613 825 303 6
Cuts at: 4823 5180 5483 5780 5948 5990 6221 6341 6413
Size: 357 303 297 168 42 231 120 72
Cuts at: 6413 6665 6941 7031 7763 8002 9137 9818 10009
Size: 252 276 90 732 239 1135 681 191
Cuts at: 10009 826
Size: 1471
Fragments arranged by size:
1471 1309 1135 825 732 681 675 613
357 303 303 297 276 252 239 231
219 191 168 120 90 72 47 42
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 7064 7421 7445 7064
Size: 357 24 10273
Fragments arranged by size:
10273 357 24
SfoI GGC'GCC
Cuts at: 3202 6101 3202
Size: 2899 7755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10654
SgrAI Cr'CCGG_yG
Cuts at: 1700 4968 1700
Size: 3268 7386
SmaI CCC'GGG
Cuts at: 1872 3347 5537 7925 7981 1872
Size: 1475 2190 2388 56 4545
Fragments arranged by size:
4545 2388 2190 1475 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2786 4547 5000 5361 5390 6644
Size: 197 926 843 1761 453 361 29 1254
Cuts at: 6644 6992 7100 7541 7661 8758 9659 9921 820
Size: 348 108 441 120 1097 901 262 1553
Fragments arranged by size:
1761 1553 1254 1097 926 901 843 453
441 361 348 262 197 120 108 29
SnaBI TAC'GTA
Cuts at: 493 8445 493
Size: 7952 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10654
SphI G_CATG'C
Cuts at: 1132 7744 8012 1132
Size: 6612 268 3774
Fragments arranged by size:
6612 3774 268
SrfI GCCC'GGGC
Cuts at: 5537 5537
Size: 10654
SspI AAT'ATT
Cuts at: 5 52 8578 5
Size: 47 8526 2081
Fragments arranged by size:
8526 2081 47
StuI AGG'CCT
Cuts at: 1517 2423 2484 2825 3333 3767 6947 7317 1517
Size: 906 61 341 508 434 3180 370 4854
Fragments arranged by size:
4854 3180 906 508 434 370 341 61
StyI C'CwwG_G
Cuts at: 513 1065 2069 2213 2817 3144 3154 3375 3423
Size: 552 1004 144 604 327 10 221 48
Cuts at: 3423 3762 4270 4434 4497 4884 5130 5874 6237
Size: 339 508 164 63 387 246 744 363
Cuts at: 6237 7602 8093 513
Size: 1365 491 3074
Fragments arranged by size:
3074 1365 1004 744 604 552 508 491
387 363 339 327 246 221 164 144
63 48 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 3835 4427 4808 5162 6314 8681
Size: 1255 647 1608 592 381 354 1152 2367
Cuts at: 8681 8698 8851 9036 9481 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2367 1608 1498 1255 1152 647 592 445
381 354 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 3639 8900
Size: 274 80 33 51 1443 729 933 5261
Cuts at: 8900 10612 96
Size: 1712 138
Fragments arranged by size:
5261 1712 1443 933 729 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3711 3743 3810 3842 4591 4623 4973 5005 6020
Size: 32 67 32 749 32 350 32 1015
Cuts at: 6020 6052 10614 10646 3711
Size: 32 4562 32 3719
Fragments arranged by size:
4562 3719 1015 749 350 67 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3662 4090 6421 1316
Size: 2346 428 2331 5549
Fragments arranged by size:
5549 2346 2331 428
XbaI T'CTAG_A
Cuts at: 7990 7990
Size: 10654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2815 4421 4891 5227 5311 5935 6133 6250
Size: 684 1606 470 336 84 624 198 117
Cuts at: 6250 6325 6745 7825 2131
Size: 75 420 1080 4960
Fragments arranged by size:
4960 1606 1080 684 624 470 420 336
198 117 84 75
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10654
XmaI C'CCGG_G
Cuts at: 1870 3345 5535 7923 7979 1870
Size: 1475 2190 2388 56 4545
Fragments arranged by size:
4545 2388 2190 1475 56
XmnI GAAnn'nnTTC
Cuts at: 8783 8783
Size: 10654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8462 276
Size: 53 83 186 936 6928 2468
Fragments arranged by size:
6928 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BplI BsiWI BstZ17I EcoRV FseI FspAI
NruI PacI PmlI PspXI PsrI RsrII SanDI SwaI