Restriction map for
pFN21AA1503
(Circular) (Six-base) MAPSORT of: pFN21AA1503.seq Check: 9012 from: 1 to: 7075
pFN21AA1503 7075 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4885 278
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 4418 1937
Size: 484 1997 4594
Fragments arranged by size:
4594 1997 484
Acc65I G'GTAC_C
Cuts at: 4396 4396
Size: 7075
AclI AA'CG_TT
Cuts at: 1563 5202 5575 1563
Size: 3639 373 3063
Fragments arranged by size:
3639 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 3050 5138 6522 6790 1632
Size: 764 654 2088 1384 268 1917
Fragments arranged by size:
2088 1917 1384 764 654 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6878
AflIII A'CryG_T
Cuts at: 1203 5974 6951 1203
Size: 4771 977 1327
Fragments arranged by size:
4771 1327 977
AgeI A'CCGG_T
Cuts at: 5906 5906
Size: 7075
AhdI GACnn_n'nnGTC
Cuts at: 3816 5804 3816
Size: 1988 5087
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2130 1184
Size: 946 6129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 7043
AlwNI CAG_nnn'CTG
Cuts at: 3424 4265 4818 6390 3424
Size: 841 553 1572 4109
Fragments arranged by size:
4109 1572 841 553
ApaI G_GGCC'C
Cuts at: 2501 2501
Size: 7075
ApaLI G'TGCA_C
Cuts at: 3656 3746 5132 6288 3656
Size: 90 1386 1156 4443
Fragments arranged by size:
4443 1386 1156 90
ApoI r'AATT_y
Cuts at: 1493 2815 3965 4384 4610 4762 6871 1493
Size: 1322 1150 419 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 1150 419 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5629 160
Size: 872 4597 1606
Fragments arranged by size:
4597 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2219 2250 3144 4355 4400 1870
Size: 73 139 137 31 894 1211 45 4545
Fragments arranged by size:
4545 1211 894 139 137 73 45 31
BamHI G'GATC_C
Cuts at: 4405 4772 4405
Size: 367 6708
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2950 3245 4396 5851 618
Size: 325 217 627 1163 295 1151 1455 1842
Fragments arranged by size:
1842 1455 1163 1151 627 325 295 217
BanII G_rGCy'C
Cuts at: 1391 2154 2220 2501 3621 4221 4356 4394 1391
Size: 763 66 281 1120 600 135 38 4072
Fragments arranged by size:
4072 1120 763 600 281 135 66 38
BbeI G_GCGC'C
Cuts at: 2954 2954
Size: 7075
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3375 928
Size: 971 1476 4628
Fragments arranged by size:
4628 1476 971
BbvCI CC'TCA_GC
Cuts at: 3433 3433
Size: 7075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5266 5300 1903
Size: 34 3329 34 3678
Fragments arranged by size:
3678 3329 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 3841 4967 6177 3347
Size: 494 1126 1210 4245
Fragments arranged by size:
4245 1210 1126 494
BclI T'GATC_A
Cuts at: 1548 3096 3426 1548
Size: 1548 330 5197
Fragments arranged by size:
5197 1548 330
BfrBI ATG'CAT
Cuts at: 2859 2859
Size: 7075
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 4360 5686
Size: 107 122 71 631 605 412 2276 1326
Cuts at: 5686 136
Size: 1525
Fragments arranged by size:
2276 1525 1326 631 605 412 122 107
71
BglII A'GATC_T
Cuts at: 2550 7070 2550
Size: 4520 2555
BlpI GC'TnA_GC
Cuts at: 2148 4492 2148
Size: 2344 4731
Bme1580I G_kGCm'C
Cuts at: 1790 2501 2658 2791 3250 3660 3750 5136 6292
Size: 711 157 133 459 410 90 1386 1156
Cuts at: 6292 1790
Size: 2573
Fragments arranged by size:
2573 1386 1156 711 459 410 157 133
90
BmgBI CAC'GTC
Cuts at: 1318 3457 1318
Size: 2139 4936
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3625 4253 4806
Size: 416 53 83 399 24 2208 628 553
Cuts at: 4806 5764 442
Size: 958 1753
Fragments arranged by size:
2208 1753 958 628 553 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 2849 3299 3425 3635 5735 1239
Size: 1430 180 450 126 210 2100 2579
Fragments arranged by size:
2579 2100 1430 450 210 180 126
Bpu10I CC'TnA_GC
Cuts at: 2169 3090 3433 4350 2169
Size: 921 343 917 4894
Fragments arranged by size:
4894 921 917 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2175 4235 5200 6065 6363 2175
Size: 2060 965 865 298 2887
Fragments arranged by size:
2887 2060 965 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2735 4318 5738 882
Size: 617 1236 1583 1420 2219
Fragments arranged by size:
2219 1583 1420 1236 617
BsaAI yAC'GTr
Cuts at: 493 1204 3625 4866 493
Size: 711 2421 1241 2702
Fragments arranged by size:
2702 2421 1241 711
BsaBI GATnn'nnATC
Cuts at: 7069 7069
Size: 7075
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2951 3670 4882 5264
Size: 53 83 186 936 1418 719 1212 382
Cuts at: 5264 5899 275
Size: 635 1451
Fragments arranged by size:
1451 1418 1212 936 719 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3638 5508 5906 6180 6327 6738 3638
Size: 1870 398 274 147 411 3975
Fragments arranged by size:
3975 1870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2825 2855 1178
Size: 30 165 30 1422 30 5398
Fragments arranged by size:
5398 1422 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2846 3978 1186
Size: 991 669 1132 4283
Fragments arranged by size:
4283 1132 991 669
BseYI C'CCAG_C
Cuts at: 2386 3204 3651 4180 4196 4287 6278 6774 2386
Size: 818 447 529 16 91 1991 496 2687
Fragments arranged by size:
2687 1991 818 529 496 447 91 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 2591 2909 3293 1163
Size: 573 762 93 318 384 4945
Fragments arranged by size:
4945 762 573 384 318 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4525 5286 6314 1540
Size: 275 186 2524 761 1028 2301
Fragments arranged by size:
2524 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 3660 3709 3750 5136 5221 6292 2154
Size: 1506 49 41 1386 85 1071 2937
Fragments arranged by size:
2937 1506 1386 1071 85 49 41
BsmI GAATG_Cn'
Cuts at: 2119 3834 4586 4679 2119
Size: 1715 752 93 4515
Fragments arranged by size:
4515 1715 752 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2220 2501 2658 2791 3250 3621
Size: 399 364 66 281 157 133 459 371
Cuts at: 3621 3660 3709 3750 4221 4356 4394 5136 5221
Size: 39 49 41 471 135 38 742 85
Cuts at: 5221 6292 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 471 459 399 371 364
281 157 135 133 85 66 49 41
39 38
BspEI T'CCGG_A
Cuts at: 3638 6738 3638
Size: 3100 3975
BspHI T'CATG_A
Cuts at: 3057 3687 4964 6694 3057
Size: 630 1277 1730 3438
Fragments arranged by size:
3438 1730 1277 630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3701 4363 4430 844
Size: 2857 662 67 3489
Fragments arranged by size:
3489 2857 662 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4962 1394
Size: 429 3139 3507
Fragments arranged by size:
3507 3139 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 5570 5744 65
Size: 1183 2606 1716 174 1396
Fragments arranged by size:
2606 1716 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5719 5906 1700
Size: 110 3909 187 2869
Fragments arranged by size:
3909 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2398 96
Size: 2302 4773
BssHII G'CGCG_C
Cuts at: 1925 3647 1925
Size: 1722 5353
BssSI C'ACGA_G
Cuts at: 5135 6147 5135
Size: 1012 6063
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2856 3316 3469 794
Size: 2062 460 153 4400
Fragments arranged by size:
4400 2062 460 153
BstBI TT'CG_AA
Cuts at: 3790 5879 3790
Size: 2089 4986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7075
BstXI CCAn_nnnn'nTGG
Cuts at: 4188 4188
Size: 7075
BstYI r'GATC_y
Cuts at: 1972 2550 2976 3544 4405 4772 5157 5174 6626
Size: 578 426 568 861 367 385 17 1452
Cuts at: 6626 6712 6724 7070 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 861 578 568 426 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2174 513
Size: 552 1109 5414
Fragments arranged by size:
5414 1109 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4194 487
Size: 683 56 2968 3368
Fragments arranged by size:
3368 2968 683 56
BtsI GCAGTG_nn'
Cuts at: 2286 4591 5385 5405 2286
Size: 2305 794 20 3956
Fragments arranged by size:
3956 2305 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7040
DraI TTT'AAA
Cuts at: 4379 4379
Size: 7075
DraIII CAC_nnn'GTG
Cuts at: 2201 3469 2201
Size: 1268 5807
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3019 6082 809
Size: 507 1703 3063 1802
Fragments arranged by size:
3063 1802 1703 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2094 3605
Size: 54 1425 50 275 18 211 53 1511
Cuts at: 3605 4150 4522 5411 7040 8
Size: 545 372 889 1629 43
Fragments arranged by size:
1629 1511 1425 889 545 372 275 211
54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4522 1537
Size: 275 2710 4090
Fragments arranged by size:
4090 2710 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 3170 3998 5005 1353
Size: 893 924 828 1007 3423
Fragments arranged by size:
3423 1007 924 893 828
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 4378 5662 6036 6182 387
Size: 1064 138 699 2090 1284 374 146 1280
Fragments arranged by size:
2090 1284 1280 1064 699 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 2152
Size: 7075
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2396 2669 2849 3050 3299 3425 3635
Size: 393 764 273 180 201 249 126 210
Cuts at: 3635 5138 5735 6522 6790 1239
Size: 1503 597 787 268 1524
Fragments arranged by size:
1524 1503 787 764 597 393 273 268
249 210 201 180 126
EcoNI CCTnn'n_nnAGG
Cuts at: 3490 3490
Size: 7075
EcoO109I rG'GnC_Cy
Cuts at: 2186 2326 2497 2498 2822 3224 3400 2186
Size: 140 171 1 324 402 176 5861
Fragments arranged by size:
5861 402 324 176 171 140 1
EcoRI G'AATT_C
Cuts at: 4384 4762 4384
Size: 378 6697
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 7075
FspI TGC'GCA
Cuts at: 5581 5581
Size: 7075
HaeII r_GCGC'y
Cuts at: 1678 2954 6222 1678
Size: 1276 3268 2531
Fragments arranged by size:
3268 2531 1276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2620 2652 3797 3802 3829
Size: 32 135 32 1048 32 1145 5 27
Cuts at: 3829 3834 5716 5748 5790 5822 1373
Size: 5 1882 32 42 32 2626
Fragments arranged by size:
2626 1882 1145 1048 135 42 32 32
32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3124 3898 4419 4665 669
Size: 988 281 1186 774 521 246 3079
Fragments arranged by size:
3079 1186 988 774 521 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7075
HpaI GTT'AAC
Cuts at: 4665 4665
Size: 7075
KasI G'GCGC_C
Cuts at: 2950 2950
Size: 7075
KpnI G_GTAC'C
Cuts at: 4400 4400
Size: 7075
MfeI C'AATT_G
Cuts at: 4674 4674
Size: 7075
MluI A'CGCG_T
Cuts at: 6951 6951
Size: 7075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2160 3825 6189 6373 1305
Size: 855 1665 2364 184 2007
Fragments arranged by size:
2364 2007 1665 855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 3607 10
Size: 54 1425 343 264 1511 3478
Fragments arranged by size:
3478 1511 1425 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 5033 5392 5551 518
Size: 648 1524 2343 359 159 2042
Fragments arranged by size:
2343 2042 1524 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2887 3204 4491 5168 6316 6561 1606
Size: 1281 317 1287 677 1148 245 2120
Fragments arranged by size:
2120 1287 1281 1148 677 317 245
NarI GG'CG_CC
Cuts at: 2951 2951
Size: 7075
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6523
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7075
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7075
NotI GC'GGCC_GC
Cuts at: 4522 4522
Size: 7075
NsiI A_TGCA'T
Cuts at: 2861 2861
Size: 7075
NspI r_CATG'y
Cuts at: 1132 1229 2395 3556 3889 4433 5978 1132
Size: 97 1166 1161 333 544 1545 2229
Fragments arranged by size:
2229 1545 1166 1161 544 333 97
PasI CC'CwG_GG
Cuts at: 1348 3250 1348
Size: 1902 5173
PciI A'CATG_T
Cuts at: 5974 5974
Size: 7075
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2454 3049 1108
Size: 1346 595 5134
Fragments arranged by size:
5134 1346 595
PfoI T'CCnGG_A
Cuts at: 2979 3339 2979
Size: 360 6715
PmeI GTTT'AAAC
Cuts at: 4379 4379
Size: 7075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 5144 5176 6657 6689 2825
Size: 32 2287 32 1481 32 3211
Fragments arranged by size:
3211 2287 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2186 2326 2822 3224 3400 2186
Size: 140 496 402 176 5861
Fragments arranged by size:
5861 496 402 176 140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7075
PsiI TTA'TAA
Cuts at: 4645 4645
Size: 7075
PspOMI G'GGCC_C
Cuts at: 2497 2497
Size: 7075
PstI C_TGCA'G
Cuts at: 830 2145 2680 3954 4374 4427 830
Size: 1315 535 1274 420 53 3478
Fragments arranged by size:
3478 1315 1274 535 420 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7075
PvuII CAG'CTG
Cuts at: 2887 2887
Size: 7075
SacI G_AGCT'C
Cuts at: 2154 2154
Size: 7075
SalI G'TCGA_C
Cuts at: 1936 4417 1936
Size: 2481 4594
SanDI GG'GwC_CC
Cuts at: 3224 3224
Size: 7075
SapI GCTCTTCn'nnn_
Cuts at: 3170 3170
Size: 7075
SbfI CC_TGCA'GG
Cuts at: 4427 4427
Size: 7075
ScaI AGT'ACT
Cuts at: 5323 7035 5323
Size: 1712 5363
SfcI C'TryA_G
Cuts at: 826 1045 2141 2331 2676 3950 4370 4423 5558
Size: 219 1096 190 345 1274 420 53 1135
Cuts at: 5558 6239 6430 826
Size: 681 191 1471
Fragments arranged by size:
1471 1274 1135 1096 681 420 345 219
191 190 53
SfoI GGC'GCC
Cuts at: 2952 2952
Size: 7075
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7075
SmaI CCC'GGG
Cuts at: 1872 4357 4402 1872
Size: 2485 45 4545
Fragments arranged by size:
4545 2485 45
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2190 4214 5179 6080 6342 820
Size: 197 926 247 2024 965 901 262 1553
Fragments arranged by size:
2024 1553 965 926 901 262 247 197
SnaBI TAC'GTA
Cuts at: 493 4866 493
Size: 4373 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7075
SphI G_CATG'C
Cuts at: 1132 2395 3889 4433 1132
Size: 1263 1494 544 3774
Fragments arranged by size:
3774 1494 1263 544
SspI AAT'ATT
Cuts at: 5 52 4999 5
Size: 47 4947 2081
Fragments arranged by size:
4947 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7075
StyI C'CwwG_G
Cuts at: 513 1065 2157 3066 4514 513
Size: 552 1092 909 1448 3074
Fragments arranged by size:
3074 1448 1092 909 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3643 5102 5119 5272 5457 5902 325
Size: 1255 2063 1459 17 153 185 445 1498
Fragments arranged by size:
2063 1498 1459 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2398 3909 5321
Size: 274 80 33 51 1443 421 1511 1412
Cuts at: 5321 7033 96
Size: 1712 138
Fragments arranged by size:
1712 1511 1443 1412 421 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 2900 2932 2961 2993 4161 4193 7035
Size: 32 601 32 29 32 1168 32 2842
Cuts at: 7035 7067 2267
Size: 32 2275
Fragments arranged by size:
2842 2275 1168 601 32 32 32 32
32 29
Tth111I GACn'n_nGTC
Cuts at: 1316 3683 1316
Size: 2367 4708
XbaI T'CTAG_A
Cuts at: 4411 4411
Size: 7075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3781 3781
Size: 7075
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7075
XmaI C'CCGG_G
Cuts at: 1870 4355 4400 1870
Size: 2485 45 4545
Fragments arranged by size:
4545 2485 45
XmnI GAAnn'nnTTC
Cuts at: 5204 5204
Size: 7075
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4883 276
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI
SrfI SwaI