Restriction map for
pFN21AA1497
(Circular) (Six-base) MAPSORT of: pFN21AA1497.seq Check: 5016 from: 1 to: 6853
pFN21AA1497 6853 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3659 4663 278
Size: 53 83 186 936 2123 1004 2468
Fragments arranged by size:
2468 2123 1004 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2419 2913 4125 4196 1937
Size: 482 494 1212 71 4594
Fragments arranged by size:
4594 1212 494 482 71
Acc65I G'GTAC_C
Cuts at: 3449 3572 4174 3449
Size: 123 602 6128
Fragments arranged by size:
6128 602 123
AclI AA'CG_TT
Cuts at: 1563 4980 5353 1563
Size: 3417 373 3063
Fragments arranged by size:
3417 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4916 6300 6568 1632
Size: 3284 1384 268 1917
Fragments arranged by size:
3284 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6656
AflIII A'CryG_T
Cuts at: 1203 3497 4146 5752 6729 1203
Size: 2294 649 1606 977 1327
Fragments arranged by size:
2294 1606 1327 977 649
AgeI A'CCGG_T
Cuts at: 5684 5684
Size: 6853
AhdI GACnn_n'nnGTC
Cuts at: 5582 5582
Size: 6853
AleI CACnn'nnGTG
Cuts at: 1166 2228 3533 1166
Size: 1062 1305 4486
Fragments arranged by size:
4486 1305 1062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6853
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3255 3287 3255
Size: 32 6821
AlwNI CAG_nnn'CTG
Cuts at: 3368 4596 6168 3368
Size: 1228 1572 4053
Fragments arranged by size:
4053 1572 1228
ApaLI G'TGCA_C
Cuts at: 4910 6066 4910
Size: 1156 5697
ApoI r'AATT_y
Cuts at: 1493 2121 2586 2619 2650 2794 2801 3372 4162
Size: 628 465 33 31 144 7 571 790
Cuts at: 4162 4388 4540 6649 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 790 628 571 465 226 152
144 33 31 7
AseI AT'TA_AT
Cuts at: 160 1032 5407 160
Size: 872 4375 1606
Fragments arranged by size:
4375 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
AvaI C'yCGr_G
Cuts at: 1870 1943 3531 4178 1870
Size: 73 1588 647 4545
Fragments arranged by size:
4545 1588 647 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3484 3517 3564 3597 3484
Size: 33 47 33 6740
Fragments arranged by size:
6740 47 33 33
BamHI G'GATC_C
Cuts at: 4183 4550 4183
Size: 367 6486
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3225 3449 3572 4174 5629
Size: 325 217 627 1438 224 123 602 1455
Cuts at: 5629 618
Size: 1842
Fragments arranged by size:
1842 1455 1438 627 602 325 224 217
123
BanII G_rGCy'C
Cuts at: 1391 2904 3186 4172 1391
Size: 1513 282 986 4072
Fragments arranged by size:
4072 1513 986 282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 2267 3486 928
Size: 971 171 197 1219 4295
Fragments arranged by size:
4295 1219 971 197 171
BbvCI CC'TCA_GC
Cuts at: 2429 2429
Size: 6853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2236 2270 2903 2937 5044 5078 1903
Size: 34 299 34 633 34 2107 34 3678
Fragments arranged by size:
3678 2107 633 299 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4745 5955 3116
Size: 1629 1210 4014
Fragments arranged by size:
4014 1629 1210
BclI T'GATC_A
Cuts at: 1548 3877 1548
Size: 2329 4524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5464 136
Size: 107 122 71 631 605 3792 1525
Fragments arranged by size:
3792 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6848 6848
Size: 6853
BlpI GC'TnA_GC
Cuts at: 3357 4270 3357
Size: 913 5940
Bme1580I G_kGCm'C
Cuts at: 1790 3228 4914 6070 1790
Size: 1438 1686 1156 2573
Fragments arranged by size:
2573 1686 1438 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6853
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2199 2888 3388
Size: 416 53 83 399 24 782 689 500
Cuts at: 3388 3722 4584 5542 442
Size: 334 862 958 1753
Fragments arranged by size:
1753 958 862 782 689 500 416 399
334 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5513 1239
Size: 4274 2579
Bpu10I CC'TnA_GC
Cuts at: 2429 2429
Size: 6853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 4978 5843 6141 2747
Size: 2231 865 298 3459
Fragments arranged by size:
3459 2231 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5516 882
Size: 617 4017 2219
Fragments arranged by size:
4017 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4644 493
Size: 711 3440 2702
Fragments arranged by size:
3440 2702 711
BsaBI GATnn'nnATC
Cuts at: 3104 6847 3104
Size: 3743 3110
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3656 4660 5042 5677
Size: 53 83 186 936 2123 1004 382 635
Cuts at: 5677 275
Size: 1451
Fragments arranged by size:
2123 1451 1004 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5286 5684 5958 6105 6516 5286
Size: 398 274 147 411 5623
Fragments arranged by size:
5623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2513 2543 3173 3203 3255
Size: 30 165 30 1110 30 630 30 52
Cuts at: 3255 3285 1178
Size: 30 4746
Fragments arranged by size:
4746 1110 630 165 52 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6853
BseYI C'CCAG_C
Cuts at: 2047 2361 6056 6552 2047
Size: 314 3695 496 2348
Fragments arranged by size:
3695 2348 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6280
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2917 4303 5064 6092 1540
Size: 275 186 916 1386 761 1028 2301
Fragments arranged by size:
2301 1386 1028 916 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2904 4914 4999 6070 2904
Size: 2010 85 1071 3687
Fragments arranged by size:
3687 2010 1071 85
BsmI GAATG_Cn'
Cuts at: 3058 4364 4457 3058
Size: 1306 93 5454
Fragments arranged by size:
5454 1306 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2904 3186 3228 4172 4914 4999 6070
Size: 399 1114 282 42 944 742 85 1071
Cuts at: 6070 1391
Size: 2174
Fragments arranged by size:
2174 1114 1071 944 742 399 282 85
42
BspEI T'CCGG_A
Cuts at: 6516 6516
Size: 6853
BspHI T'CATG_A
Cuts at: 2592 3651 4742 6472 2592
Size: 1059 1091 1730 2973
Fragments arranged by size:
2973 1730 1091 1059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4208 844
Size: 3364 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4740 1394
Size: 429 2917 3507
Fragments arranged by size:
3507 2917 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2185 3945 5348 5522 65
Size: 1183 937 1760 1403 174 1396
Fragments arranged by size:
1760 1403 1396 1183 937 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5497 5684 1700
Size: 110 3687 187 2869
Fragments arranged by size:
3687 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6853
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6853
BssSI C'ACGA_G
Cuts at: 4913 5925 4913
Size: 1012 5841
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6853
BstBI TT'CG_AA
Cuts at: 5657 5657
Size: 6853
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6853
BstYI r'GATC_y
Cuts at: 1972 4183 4550 4935 4952 6404 6490 6502 6848
Size: 2211 367 385 17 1452 86 12 346
Cuts at: 6848 1972
Size: 1977
Fragments arranged by size:
2211 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3122 3122
Size: 6853
BtgI C'CryG_G
Cuts at: 513 1065 2004 3351 513
Size: 552 939 1347 4015
Fragments arranged by size:
4015 1347 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2239 487
Size: 683 56 1013 5101
Fragments arranged by size:
5101 1013 683 56
BtsI GCAGTG_nn'
Cuts at: 3870 4369 5163 5183 3870
Size: 499 794 20 5540
Fragments arranged by size:
5540 794 499 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2152 2187 529
Size: 35 1588 35 5195
Fragments arranged by size:
5195 1588 35 35
DraI TTT'AAA
Cuts at: 4157 4157
Size: 6853
DraIII CAC_nnn'GTG
Cuts at: 3232 3232
Size: 6853
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3823 5860 809
Size: 507 2507 2037 1802
Fragments arranged by size:
2507 2037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4300 5189 6818
Size: 54 1425 50 275 18 2470 889 1629
Cuts at: 6818 8
Size: 43
Fragments arranged by size:
2470 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4300 1537
Size: 275 2488 4090
Fragments arranged by size:
4090 2488 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4027 4783 1353
Size: 2674 756 3423
Fragments arranged by size:
3423 2674 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5440 5814 5960 387
Size: 1064 138 3851 374 146 1280
Fragments arranged by size:
3851 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2902 2902
Size: 6853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4916 5513 6300 6568 1239
Size: 393 3284 597 787 268 1524
Fragments arranged by size:
3284 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3558 3724 3558
Size: 166 6687
EcoRI G'AATT_C
Cuts at: 2619 4162 4540 2619
Size: 1543 378 4932
Fragments arranged by size:
4932 1543 378
EcoRV GAT'ATC
Cuts at: 3282 3319 3282
Size: 37 6816
FspI TGC'GCA
Cuts at: 5359 5359
Size: 6853
HaeII r_GCGC'y
Cuts at: 1678 6000 1678
Size: 4322 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2752 3173 3205 5494
Size: 32 135 32 1148 32 421 32 2289
Cuts at: 5494 5526 5568 5600 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2289 1148 421 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2148 2914 3553 4126 4197 4443
Size: 988 281 210 766 639 573 71 246
Cuts at: 4443 669
Size: 3079
Fragments arranged by size:
3079 988 766 639 573 281 246 210
71
HindIII A'AGCT_T
Cuts at: 748 2659 3025 748
Size: 1911 366 4576
Fragments arranged by size:
4576 1911 366
HpaI GTT'AAC
Cuts at: 3553 4443 3553
Size: 890 5963
KpnI G_GTAC'C
Cuts at: 3453 3576 4178 3453
Size: 123 602 6128
Fragments arranged by size:
6128 602 123
MfeI C'AATT_G
Cuts at: 2039 4452 2039
Size: 2413 4440
MluI A'CGCG_T
Cuts at: 6729 6729
Size: 6853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 3180 5967 6151 1305
Size: 1251 624 2787 184 2007
Fragments arranged by size:
2787 2007 1251 624 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5031
Fragments arranged by size:
5031 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 3533 3623 3950 4811 5170 5329
Size: 648 1062 1305 90 327 861 359 159
Cuts at: 5329 518
Size: 2042
Fragments arranged by size:
2042 1305 1062 861 648 359 327 159
90
MspA1I CmG'CkG
Cuts at: 1606 4269 4946 6094 6339 1606
Size: 2663 677 1148 245 2120
Fragments arranged by size:
2663 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3351 513
Size: 552 939 1347 4015
Fragments arranged by size:
4015 1347 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6853
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6853
NotI GC'GGCC_GC
Cuts at: 4300 4300
Size: 6853
NruI TCG'CGA
Cuts at: 2258 2258
Size: 6853
NspI r_CATG'y
Cuts at: 1132 1229 3501 4150 4211 5756 1132
Size: 97 2272 649 61 1545 2229
Fragments arranged by size:
2272 2229 1545 649 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6853
PciI A'CATG_T
Cuts at: 3497 4146 5752 3497
Size: 649 1606 4598
Fragments arranged by size:
4598 1606 649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2995 1108
Size: 1887 4966
PfoI T'CCnGG_A
Cuts at: 2695 3249 3629 2695
Size: 554 380 5919
Fragments arranged by size:
5919 554 380
PmeI GTTT'AAAC
Cuts at: 4157 4157
Size: 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3255 3287 4922 4954 6435 6467 3255
Size: 32 1635 32 1481 32 3641
Fragments arranged by size:
3641 1635 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3558 3724 3558
Size: 166 6687
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6853
PsiI TTA'TAA
Cuts at: 4423 4423
Size: 6853
PstI C_TGCA'G
Cuts at: 830 2280 3765 3907 4105 4205 830
Size: 1450 1485 142 198 100 3478
Fragments arranged by size:
3478 1485 1450 198 142 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6853
SacI G_AGCT'C
Cuts at: 2904 2904
Size: 6853
SalI G'TCGA_C
Cuts at: 1936 2912 4124 4195 1936
Size: 976 1212 71 4594
Fragments arranged by size:
4594 1212 976 71
SanDI GG'GwC_CC
Cuts at: 3558 3724 3558
Size: 166 6687
SbfI CC_TGCA'GG
Cuts at: 4205 4205
Size: 6853
ScaI AGT'ACT
Cuts at: 5101 6813 5101
Size: 1712 5141
SexAI A'CCwGG_T
Cuts at: 3228 3228
Size: 6853
SfcI C'TryA_G
Cuts at: 826 1045 2276 3761 3903 4101 4201 5336 6017
Size: 219 1231 1485 142 198 100 1135 681
Cuts at: 6017 6208 826
Size: 191 1471
Fragments arranged by size:
1485 1471 1231 1135 681 219 198 191
142 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6853
SmaI CCC'GGG
Cuts at: 1872 4180 1872
Size: 2308 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 4957 5858 6120 820
Size: 197 926 783 2231 901 262 1553
Fragments arranged by size:
2231 1553 926 901 783 262 197
SnaBI TAC'GTA
Cuts at: 493 4644 493
Size: 4151 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6853
SphI G_CATG'C
Cuts at: 1132 4211 1132
Size: 3079 3774
SspI AAT'ATT
Cuts at: 5 52 3805 4777 5
Size: 47 3753 972 2081
Fragments arranged by size:
3753 2081 972 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6853
StyI C'CwwG_G
Cuts at: 513 1065 2004 3351 3453 3517 4292 513
Size: 552 939 1347 102 64 775 3074
Fragments arranged by size:
3074 1347 939 775 552 102 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2590 4880 4897 5050 5235 5680 325
Size: 1255 1010 2290 17 153 185 445 1498
Fragments arranged by size:
2290 1498 1255 1010 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 3954 5099
Size: 274 80 33 51 1443 1913 64 1145
Cuts at: 5099 6811 96
Size: 1712 138
Fragments arranged by size:
1913 1712 1443 1145 274 138 80 64
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2513 2545 2677 2709 6813 6845 2513
Size: 32 132 32 4104 32 2521
Fragments arranged by size:
4104 2521 132 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6853
XbaI T'CTAG_A
Cuts at: 4189 4189
Size: 6853
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6853
XmaI C'CCGG_G
Cuts at: 1870 4178 1870
Size: 2308 4545
XmnI GAAnn'nnTTC
Cuts at: 4982 4982
Size: 6853
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3657 4661 276
Size: 53 83 186 936 2123 1004 2468
Fragments arranged by size:
2468 2123 1004 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SapI SfiI SfoI
SrfI SwaI XcmI