Restriction map for
pFN21AA1496
(Circular) (Six-base) MAPSORT of: pFN21AA1496.seq Check: 444 from: 1 to: 7789
pFN21AA1496 7789 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3259 3446 3259
Size: 187 7602
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5599 278
Size: 53 83 186 936 4063 2468
Fragments arranged by size:
4063 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5132 1937
Size: 3195 4594
Acc65I G'GTAC_C
Cuts at: 5110 5110
Size: 7789
AclI AA'CG_TT
Cuts at: 1563 5916 6289 1563
Size: 4353 373 3063
Fragments arranged by size:
4353 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 4110 4140 4200 4631 4863 4998 5852
Size: 704 1774 30 60 431 232 135 854
Cuts at: 5852 7236 7504 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1774 1384 854 704 431 268 232
135 60 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7592
AflIII A'CryG_T
Cuts at: 1203 2588 6688 7665 1203
Size: 1385 4100 977 1327
Fragments arranged by size:
4100 1385 1327 977
AgeI A'CCGG_T
Cuts at: 6620 6620
Size: 7789
AhdI GACnn_n'nnGTC
Cuts at: 4839 6518 4839
Size: 1679 6110
AleI CACnn'nnGTG
Cuts at: 1166 2591 3947 4757 1166
Size: 1425 1356 810 4198
Fragments arranged by size:
4198 1425 1356 810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7789
AlwNI CAG_nnn'CTG
Cuts at: 2402 3953 3986 4178 5532 7104 2402
Size: 1551 33 192 1354 1572 3087
Fragments arranged by size:
3087 1572 1551 1354 192 33
ApaI G_GGCC'C
Cuts at: 3625 3625
Size: 7789
ApaLI G'TGCA_C
Cuts at: 5846 7002 5846
Size: 1156 6633
ApoI r'AATT_y
Cuts at: 1493 2314 2371 2905 3369 4019 5098 5324 5476
Size: 821 57 534 464 650 1079 226 152
Cuts at: 5476 7585 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1079 821 650 534 464 226
152 57
AseI AT'TA_AT
Cuts at: 160 1032 2265 2781 6343 160
Size: 872 1233 516 3562 1606
Fragments arranged by size:
3562 1606 1233 872 516
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7789
AvaI C'yCGr_G
Cuts at: 1870 1943 2619 5114 1870
Size: 73 676 2495 4545
Fragments arranged by size:
4545 2495 676 73
BamHI G'GATC_C
Cuts at: 4168 4248 5006 5119 5486 4168
Size: 80 758 113 367 6471
Fragments arranged by size:
6471 758 367 113 80
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2541 3110 3517 5110 6565
Size: 325 217 627 754 569 407 1593 1455
Cuts at: 6565 618
Size: 1842
Fragments arranged by size:
1842 1593 1455 754 627 569 407 325
217
BanII G_rGCy'C
Cuts at: 1391 2633 3207 3625 5108 1391
Size: 1242 574 418 1483 4072
Fragments arranged by size:
4072 1483 1242 574 418
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3977 928
Size: 971 2078 4740
Fragments arranged by size:
4740 2078 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5980 6014 1903
Size: 34 4043 34 3678
Fragments arranged by size:
4043 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2278 5681 6891 2278
Size: 3403 1210 3176
Fragments arranged by size:
3403 3176 1210
BclI T'GATC_A
Cuts at: 1548 3693 1548
Size: 2145 5644
BfrBI ATG'CAT
Cuts at: 2895 2895
Size: 7789
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6400 136
Size: 107 122 71 631 605 4728 1525
Fragments arranged by size:
4728 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7784 7784
Size: 7789
BlpI GC'TnA_GC
Cuts at: 3201 5206 3201
Size: 2005 5784
Bme1580I G_kGCm'C
Cuts at: 1790 2544 3115 3322 3625 5850 7006 1790
Size: 754 571 207 303 2225 1156 2573
Fragments arranged by size:
2573 2225 1156 754 571 303 207
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7789
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3878 4370 4520
Size: 416 53 83 399 24 2461 492 150
Cuts at: 4520 4707 5520 6478 442
Size: 187 813 958 1753
Fragments arranged by size:
2461 1753 958 813 492 416 399 187
150 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7789
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2954
Size: 7789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 3210 6449 1239
Size: 1236 735 3239 2579
Fragments arranged by size:
3239 2579 1236 735
Bpu10I CC'TnA_GC
Cuts at: 2053 2053
Size: 7789
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4410 5914 6779 7077 4410
Size: 1504 865 298 5122
Fragments arranged by size:
5122 1504 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6452 882
Size: 617 4953 2219
Fragments arranged by size:
4953 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5580 493
Size: 711 4376 2702
Fragments arranged by size:
4376 2702 711
BsaBI GATnn'nnATC
Cuts at: 7783 7783
Size: 7789
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2935 5596 5978 6613
Size: 53 83 186 936 1402 2661 382 635
Cuts at: 6613 275
Size: 1451
Fragments arranged by size:
2661 1451 1402 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6222 6620 6894 7041 7452 6222
Size: 398 274 147 411 6559
Fragments arranged by size:
6559 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2779 2809 4784 4814 1178
Size: 30 165 30 1376 30 1975 30 4153
Fragments arranged by size:
4153 1975 1376 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2404 3101 4146 1186
Size: 1218 697 1045 4829
Fragments arranged by size:
4829 1218 1045 697
BseYI C'CCAG_C
Cuts at: 2099 2626 4664 6992 7488 2099
Size: 527 2038 2328 496 2400
Fragments arranged by size:
2400 2328 2038 527 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3287 3374 1163
Size: 573 1551 87 5578
Fragments arranged by size:
5578 1551 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5239 6000 7028 1540
Size: 275 186 3238 761 1028 2301
Fragments arranged by size:
3238 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3207 5850 5935 7006 3207
Size: 2643 85 1071 3990
Fragments arranged by size:
3990 2643 1071 85
BsmI GAATG_Cn'
Cuts at: 2897 3023 4747 5300 5393 2897
Size: 126 1724 553 93 5293
Fragments arranged by size:
5293 1724 553 126 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2942 2942
Size: 7789
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2544 2633 3115 3207 3322 3625 5108
Size: 399 754 89 482 92 115 303 1483
Cuts at: 5108 5850 5935 7006 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1483 1071 754 742 482 399 303
115 92 89 85
BspEI T'CCGG_A
Cuts at: 7452 7452
Size: 7789
BspHI T'CATG_A
Cuts at: 5678 7408 5678
Size: 1730 6059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 3259 3446 5144 844
Size: 2026 389 187 1698 3489
Fragments arranged by size:
3489 2026 1698 389 187
BsrBI CCG'CTC
Cuts at: 1394 1823 5676 1394
Size: 429 3853 3507
Fragments arranged by size:
3853 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2895 4504 6284 6458 65
Size: 1183 1647 1609 1780 174 1396
Fragments arranged by size:
1780 1647 1609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4143 4658 6433 6620 1700
Size: 110 2333 515 1775 187 2869
Fragments arranged by size:
2869 2333 1775 515 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7789
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7789
BssSI C'ACGA_G
Cuts at: 2910 5849 6861 2910
Size: 2939 1012 3838
Fragments arranged by size:
3838 2939 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7789
BstBI TT'CG_AA
Cuts at: 6593 6593
Size: 7789
BstEII G'GTnAC_C
Cuts at: 1184 4237 1184
Size: 3053 4736
BstYI r'GATC_y
Cuts at: 1972 2475 3647 4168 4248 5006 5119 5486 5871
Size: 503 1172 521 80 758 113 367 385
Cuts at: 5871 5888 7340 7426 7438 7784 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1172 758 521 503 385 367
346 113 86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 3954 3954
Size: 7789
BtgI C'CryG_G
Cuts at: 513 1065 2015 2994 3924 4004 4788 513
Size: 552 950 979 930 80 784 3514
Fragments arranged by size:
3514 979 950 930 784 552 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7050
Fragments arranged by size:
7050 683 56
BtsI GCAGTG_nn'
Cuts at: 5305 6099 6119 5305
Size: 794 20 6975
Fragments arranged by size:
6975 794 20
ClaI AT'CG_AT
Cuts at: 1554 3963 1554
Size: 2409 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3973 4008 529
Size: 35 3409 35 4310
Fragments arranged by size:
4310 3409 35 35
DraI TTT'AAA
Cuts at: 2377 5093 2377
Size: 2716 5073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6796 809
Size: 507 5480 1802
Fragments arranged by size:
5480 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5236 6125 7754
Size: 54 1425 50 275 18 3406 889 1629
Cuts at: 7754 8
Size: 43
Fragments arranged by size:
3406 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5236 1537
Size: 275 3424 4090
Fragments arranged by size:
4090 3424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2718 3331 4086 4398 4607 5719 1353
Size: 1365 613 755 312 209 1112 3423
Fragments arranged by size:
3423 1365 1112 755 613 312 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4152 4214 6376 6750 6896 387
Size: 1064 138 2563 62 2162 374 146 1280
Fragments arranged by size:
2563 2162 1280 1064 374 146 138 62
EcoICRI GAG'CTC
Cuts at: 3205 3205
Size: 7789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2475 3210 4110 4140 4200 4631
Size: 393 704 139 735 900 30 60 431
Cuts at: 4631 4863 4998 5852 6449 7236 7504 1239
Size: 232 135 854 597 787 268 1524
Fragments arranged by size:
1524 900 854 787 735 704 597 431
393 268 232 139 135 60 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3313 3313
Size: 7789
EcoO109I rG'GnC_Cy
Cuts at: 2078 3621 3622 4606 2078
Size: 1543 1 984 5261
Fragments arranged by size:
5261 1543 984 1
EcoRI G'AATT_C
Cuts at: 2905 5098 5476 2905
Size: 2193 378 5218
Fragments arranged by size:
5218 2193 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2366 3390 3528 4781 2366
Size: 1024 138 1253 5374
Fragments arranged by size:
5374 1253 1024 138
FspI TGC'GCA
Cuts at: 6295 6295
Size: 7789
HaeII r_GCGC'y
Cuts at: 1678 4665 4680 6936 1678
Size: 2987 15 2256 2531
Fragments arranged by size:
2987 2531 2256 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4541 4573 4784 4816 4825
Size: 32 135 32 2969 32 211 32 9
Cuts at: 4825 4857 6430 6462 6504 6536 1373
Size: 32 1573 32 42 32 2626
Fragments arranged by size:
2969 2626 1573 211 135 42 32 32
32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 5133 5379 669
Size: 988 281 3195 246 3079
Fragments arranged by size:
3195 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7789
HpaI GTT'AAC
Cuts at: 5379 5379
Size: 7789
KpnI G_GTAC'C
Cuts at: 5114 5114
Size: 7789
MfeI C'AATT_G
Cuts at: 2328 5388 2328
Size: 3060 4729
MluI A'CGCG_T
Cuts at: 7665 7665
Size: 7789
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2495 2660 2890 6903 7087 1305
Size: 1190 165 230 4013 184 2007
Fragments arranged by size:
4013 2007 1190 230 184 165
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5967
Fragments arranged by size:
5967 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 3440 3947 4757 5747 6106 6265
Size: 648 1425 849 507 810 990 359 159
Cuts at: 6265 518
Size: 2042
Fragments arranged by size:
2042 1425 990 849 810 648 507 359
159
MspA1I CmG'CkG
Cuts at: 1606 2194 3586 3709 4883 5205 5882 7030 7275
Size: 588 1392 123 1174 322 677 1148 245
Cuts at: 7275 1606
Size: 2120
Fragments arranged by size:
2120 1392 1174 1148 677 588 322 245
123
NaeI GCC'GGC
Cuts at: 4660 4660
Size: 7789
NcoI C'CATG_G
Cuts at: 513 1065 2015 4004 4788 513
Size: 552 950 1989 784 3514
Fragments arranged by size:
3514 1989 950 784 552
NdeI CA'TA_TG
Cuts at: 387 2467 4324 387
Size: 2080 1857 3852
Fragments arranged by size:
3852 2080 1857
NgoMIV G'CCGG_C
Cuts at: 4658 4658
Size: 7789
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7789
NotI GC'GGCC_GC
Cuts at: 5236 5236
Size: 7789
NsiI A_TGCA'T
Cuts at: 2897 2897
Size: 7789
NspI r_CATG'y
Cuts at: 1132 1229 2592 3154 5147 6692 1132
Size: 97 1363 562 1993 1545 2229
Fragments arranged by size:
2229 1993 1545 1363 562 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7789
PciI A'CATG_T
Cuts at: 2588 6688 2588
Size: 4100 3689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2453 4258 4485 1108
Size: 1345 1805 227 4412
Fragments arranged by size:
4412 1805 1345 227
PfoI T'CCnGG_A
Cuts at: 3329 3329
Size: 7789
PmeI GTTT'AAAC
Cuts at: 5093 5093
Size: 7789
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5858 5890 7371 7403 5858
Size: 32 1481 32 6244
Fragments arranged by size:
6244 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7789
PsiI TTA'TAA
Cuts at: 2235 4347 5359 2235
Size: 2112 1012 4665
Fragments arranged by size:
4665 2112 1012
PspOMI G'GGCC_C
Cuts at: 3621 3621
Size: 7789
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4050 4082 4050
Size: 32 7757
PstI C_TGCA'G
Cuts at: 830 4399 5141 830
Size: 3569 742 3478
Fragments arranged by size:
3569 3478 742
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7789
PvuII CAG'CTG
Cuts at: 2194 3709 4883 2194
Size: 1515 1174 5100
Fragments arranged by size:
5100 1515 1174
SacI G_AGCT'C
Cuts at: 3207 3207
Size: 7789
SalI G'TCGA_C
Cuts at: 1936 5131 1936
Size: 3195 4594
SapI GCTCTTCn'nnn_
Cuts at: 4398 4607 4398
Size: 209 7580
SbfI CC_TGCA'GG
Cuts at: 5141 5141
Size: 7789
ScaI AGT'ACT
Cuts at: 4860 5061 6037 7749 4860
Size: 201 976 1712 4900
Fragments arranged by size:
4900 1712 976 201
SexAI A'CCwGG_T
Cuts at: 3605 3605
Size: 7789
SfcI C'TryA_G
Cuts at: 826 1045 3987 4187 4395 4410 4828 5055 5137
Size: 219 2942 200 208 15 418 227 82
Cuts at: 5137 6272 6953 7144 826
Size: 1135 681 191 1471
Fragments arranged by size:
2942 1471 1135 681 418 227 219 208
200 191 82 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7789
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7789
SmaI CCC'GGG
Cuts at: 1872 5116 1872
Size: 3244 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4425 5893 6794 7056 820
Size: 197 926 2482 1468 901 262 1553
Fragments arranged by size:
2482 1553 1468 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5580 493
Size: 5087 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7789
SphI G_CATG'C
Cuts at: 1132 5147 1132
Size: 4015 3774
SspI AAT'ATT
Cuts at: 5 52 5713 5
Size: 47 5661 2081
Fragments arranged by size:
5661 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7789
StyI C'CwwG_G
Cuts at: 513 1065 2015 2758 3314 4004 4484 4788 5228
Size: 552 950 743 556 690 480 304 440
Cuts at: 5228 513
Size: 3074
Fragments arranged by size:
3074 950 743 690 556 552 480 440
304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2660 3997 4328 5816 5833 5986 6171
Size: 1255 1080 1337 331 1488 17 153 185
Cuts at: 6171 6616 325
Size: 445 1498
Fragments arranged by size:
1498 1488 1337 1255 1080 445 331 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2393 4858 5059
Size: 274 80 33 51 1443 416 2465 201
Cuts at: 5059 6035 7747 96
Size: 976 1712 138
Fragments arranged by size:
2465 1712 1443 976 416 274 201 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2241 2273 2779 2811 2824 2856 4525 4557 4945
Size: 32 506 32 13 32 1669 32 388
Cuts at: 4945 4977 7749 7781 2241
Size: 32 2772 32 2249
Fragments arranged by size:
2772 2249 1669 506 388 32 32 32
32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7789
XbaI T'CTAG_A
Cuts at: 5125 5125
Size: 7789
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7789
XmaI C'CCGG_G
Cuts at: 1870 5114 1870
Size: 3244 4545
XmnI GAAnn'nnTTC
Cuts at: 2712 5918 2712
Size: 3206 4583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5597 276
Size: 53 83 186 936 4063 2468
Fragments arranged by size:
4063 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NarI
NruI PacI PmlI PpuMI PspXI RsrII SacII SanDI
SfiI SfoI SrfI SwaI XcmI