Restriction map for
pFN21AA1492
(Circular) (Six-base) MAPSORT of: pFN21AA1492.seq Check: 783 from: 1 to: 7072
pFN21AA1492 7072 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4882 278
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2441 4242 4415 1937
Size: 504 1801 173 4594
Fragments arranged by size:
4594 1801 504 173
Acc65I G'GTAC_C
Cuts at: 2764 4393 2764
Size: 1629 5443
AclI AA'CG_TT
Cuts at: 1563 5199 5572 1563
Size: 3636 373 3063
Fragments arranged by size:
3636 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3375 3566 3581 4297 5135 6519 6787 1632
Size: 1743 191 15 716 838 1384 268 1917
Fragments arranged by size:
1917 1743 1384 838 716 268 191 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6875
AflIII A'CryG_T
Cuts at: 1203 2287 5971 6948 1203
Size: 1084 3684 977 1327
Fragments arranged by size:
3684 1327 1084 977
AgeI A'CCGG_T
Cuts at: 5903 5903
Size: 7072
AhdI GACnn_n'nnGTC
Cuts at: 5801 5801
Size: 7072
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2630 3557 1184
Size: 1446 927 4699
Fragments arranged by size:
4699 1446 927
AlwNI CAG_nnn'CTG
Cuts at: 2557 3149 3808 4815 6387 2557
Size: 592 659 1007 1572 3242
Fragments arranged by size:
3242 1572 1007 659 592
ApaLI G'TGCA_C
Cuts at: 5129 6285 5129
Size: 1156 5916
ApoI r'AATT_y
Cuts at: 1493 2170 3131 3173 3294 3813 3878 4307 4381
Size: 677 961 42 121 519 65 429 74
Cuts at: 4381 4607 4759 6868 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 961 677 519 429 226 152
121 74 65 42
AseI AT'TA_AT
Cuts at: 160 1032 4142 4196 5626 160
Size: 872 3110 54 1430 1606
Fragments arranged by size:
3110 1606 1430 872 54
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
AvaI C'yCGr_G
Cuts at: 1870 1943 4397 1870
Size: 73 2454 4545
Fragments arranged by size:
4545 2454 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2247 2280 2247
Size: 33 7039
BamHI G'GATC_C
Cuts at: 3995 4402 4769 3995
Size: 407 367 6298
Fragments arranged by size:
6298 407 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2764 4393 5848 618
Size: 325 217 627 977 1629 1455 1842
Fragments arranged by size:
1842 1629 1455 977 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2907 3108 4391 1391
Size: 1516 201 1283 4072
Fragments arranged by size:
4072 1516 1283 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 928
Size: 971 306 5795
Fragments arranged by size:
5795 971 306
BbvCI CC'TCA_GC
Cuts at: 4036 4036
Size: 7072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5263 5297 1903
Size: 34 50 34 3242 34 3678
Fragments arranged by size:
3678 3242 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2814 2832 2838 4964 6174 2814
Size: 18 6 2126 1210 3712
Fragments arranged by size:
3712 2126 1210 18 6
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7072
BfrBI ATG'CAT
Cuts at: 3378 3378
Size: 7072
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5683 136
Size: 107 122 71 631 605 4011 1525
Fragments arranged by size:
4011 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3264 7067 3264
Size: 3803 3269
BlpI GC'TnA_GC
Cuts at: 4489 4489
Size: 7072
Bme1580I G_kGCm'C
Cuts at: 1790 5133 6289 1790
Size: 3343 1156 2573
Fragments arranged by size:
3343 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3167 1318
Size: 1849 5223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 3259 3473
Size: 416 53 83 399 24 640 1202 214
Cuts at: 3473 3742 4803 5761 442
Size: 269 1061 958 1753
Fragments arranged by size:
1753 1202 1061 958 640 416 399 269
214 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 2814 4239 5732 1239
Size: 1245 330 1425 1493 2579
Fragments arranged by size:
2579 1493 1425 1245 330
Bpu10I CC'TnA_GC
Cuts at: 4036 4036
Size: 7072
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5197 6062 6360 5197
Size: 865 298 5909
Fragments arranged by size:
5909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3026 5735 882
Size: 617 1527 2709 2219
Fragments arranged by size:
2709 2219 1527 617
BsaAI yAC'GTr
Cuts at: 493 1204 3081 4863 493
Size: 711 1877 1782 2702
Fragments arranged by size:
2702 1877 1782 711
BsaBI GATnn'nnATC
Cuts at: 3263 7066 3263
Size: 3803 3269
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4879 5261 5896 275
Size: 53 83 186 936 3346 382 635 1451
Fragments arranged by size:
3346 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5505 5903 6177 6324 6735 5505
Size: 398 274 147 411 5842
Fragments arranged by size:
5842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3095 3125 3890 3920 1178
Size: 30 165 30 1692 30 765 30 4330
Fragments arranged by size:
4330 1692 765 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3116 1186
Size: 1930 5142
BseYI C'CCAG_C
Cuts at: 3508 6275 6771 3508
Size: 2767 496 3809
Fragments arranged by size:
3809 2767 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6499
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4522 5283 6311 1540
Size: 275 186 2521 761 1028 2301
Fragments arranged by size:
2521 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2907 3311 5133 5218 6289 2907
Size: 404 1822 85 1071 3690
Fragments arranged by size:
3690 1822 1071 404 85
BsmI GAATG_Cn'
Cuts at: 2692 4583 4676 2692
Size: 1891 93 5088
Fragments arranged by size:
5088 1891 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3114 3114
Size: 7072
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2907 3108 3311 4391 5133 5218 6289
Size: 399 1117 201 203 1080 742 85 1071
Cuts at: 6289 1391
Size: 2174
Fragments arranged by size:
2174 1117 1080 1071 742 399 203 201
85
BspEI T'CCGG_A
Cuts at: 6735 6735
Size: 7072
BspHI T'CATG_A
Cuts at: 3393 4961 6691 3393
Size: 1568 1730 3774
Fragments arranged by size:
3774 1730 1568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 4228 4427 844
Size: 1984 1400 199 3489
Fragments arranged by size:
3489 1984 1400 199
BsrBI CCG'CTC
Cuts at: 1394 1823 4959 1394
Size: 429 3136 3507
Fragments arranged by size:
3507 3136 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2612 5567 5741 65
Size: 1183 1364 2955 174 1396
Fragments arranged by size:
2955 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5716 5903 1700
Size: 110 3906 187 2869
Fragments arranged by size:
3906 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3339 3407 96
Size: 3243 68 3761
Fragments arranged by size:
3761 3243 68
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7072
BssSI C'ACGA_G
Cuts at: 5132 6144 5132
Size: 1012 6060
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7072
BstBI TT'CG_AA
Cuts at: 5876 5876
Size: 7072
BstEII G'GTnAC_C
Cuts at: 1184 2715 1184
Size: 1531 5541
BstXI CCAn_nnnn'nTGG
Cuts at: 2980 2980
Size: 7072
BstYI r'GATC_y
Cuts at: 1972 3264 3995 4402 4769 5154 5171 6623 6709
Size: 1292 731 407 367 385 17 1452 86
Cuts at: 6709 6721 7067 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1292 731 407 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2442 2442
Size: 7072
Bsu36I CC'TnA_GG
Cuts at: 2832 2832
Size: 7072
BtgI C'CryG_G
Cuts at: 513 1065 2023 2772 3999 513
Size: 552 958 749 1227 3586
Fragments arranged by size:
3586 1227 958 749 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2686 487
Size: 683 56 1460 4873
Fragments arranged by size:
4873 1460 683 56
BtsI GCAGTG_nn'
Cuts at: 4588 5382 5402 4588
Size: 794 20 6258
Fragments arranged by size:
6258 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7037
DraI TTT'AAA
Cuts at: 2391 2926 3988 4376 2391
Size: 535 1062 388 5087
Fragments arranged by size:
5087 1062 535 388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6079 809
Size: 507 4763 1802
Fragments arranged by size:
4763 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4519 5408 7037
Size: 54 1425 50 275 18 2689 889 1629
Cuts at: 7037 8
Size: 43
Fragments arranged by size:
2689 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4519 1537
Size: 275 2707 4090
Fragments arranged by size:
4090 2707 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2673 3675 4268 5002 1353
Size: 1320 1002 593 734 3423
Fragments arranged by size:
3423 1320 1002 734 593
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5659 6033 6179 387
Size: 1064 138 4070 374 146 1280
Fragments arranged by size:
4070 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2905 2905
Size: 7072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2814 3375 3566 3581 4239 4297
Size: 393 852 330 561 191 15 658 58
Cuts at: 4297 5135 5732 6519 6787 1239
Size: 838 597 787 268 1524
Fragments arranged by size:
1524 852 838 787 658 597 561 393
330 268 191 58 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2835 2835
Size: 7072
EcoO109I rG'GnC_Cy
Cuts at: 3475 3475
Size: 7072
EcoRI G'AATT_C
Cuts at: 4381 4759 4381
Size: 378 6694
FspI TGC'GCA
Cuts at: 2009 5578 2009
Size: 3569 3503
HaeII r_GCGC'y
Cuts at: 1678 6219 1678
Size: 4541 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3056 3088 4267 4299 5713
Size: 32 135 32 1484 32 1179 32 1414
Cuts at: 5713 5745 5787 5819 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1484 1414 1179 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4416 4662 669
Size: 988 281 2478 246 3079
Fragments arranged by size:
3079 2478 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3980 748
Size: 3232 3840
HpaI GTT'AAC
Cuts at: 4662 4662
Size: 7072
KpnI G_GTAC'C
Cuts at: 2768 4397 2768
Size: 1629 5443
MfeI C'AATT_G
Cuts at: 4671 4671
Size: 7072
MluI A'CGCG_T
Cuts at: 6948 6948
Size: 7072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4204 6186 6370 1305
Size: 2899 1982 184 2007
Fragments arranged by size:
2899 2007 1982 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5250
Fragments arranged by size:
5250 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 3188 3752 5030 5389 5548 518
Size: 648 966 1056 564 1278 359 159 2042
Fragments arranged by size:
2042 1278 1056 966 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2557 3337 3706 3872 4488 5165 6313
Size: 419 532 780 369 166 616 677 1148
Cuts at: 6313 6558 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 780 677 616 532 419 369
245 166
NcoI C'CATG_G
Cuts at: 513 1065 2772 513
Size: 552 1707 4813
Fragments arranged by size:
4813 1707 552
NdeI CA'TA_TG
Cuts at: 387 2410 2452 387
Size: 2023 42 5007
Fragments arranged by size:
5007 2023 42
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7072
NotI GC'GGCC_GC
Cuts at: 4519 4519
Size: 7072
NsiI A_TGCA'T
Cuts at: 3380 3380
Size: 7072
NspI r_CATG'y
Cuts at: 1132 1229 2291 4430 5975 1132
Size: 97 1062 2139 1545 2229
Fragments arranged by size:
2229 2139 1545 1062 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7072
PciI A'CATG_T
Cuts at: 2287 5971 2287
Size: 3684 3388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7072
PmeI GTTT'AAAC
Cuts at: 4376 4376
Size: 7072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5141 5173 6654 6686 5141
Size: 32 1481 32 5527
Fragments arranged by size:
5527 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3475 3475
Size: 7072
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7072
PsiI TTA'TAA
Cuts at: 4642 4642
Size: 7072
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3606 3638 3606
Size: 32 7040
PstI C_TGCA'G
Cuts at: 830 4424 830
Size: 3594 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7072
PvuII CAG'CTG
Cuts at: 2557 3337 3706 3872 2557
Size: 780 369 166 5757
Fragments arranged by size:
5757 780 369 166
SacI G_AGCT'C
Cuts at: 2907 2907
Size: 7072
SacII CC_GC'GG
Cuts at: 2026 2026
Size: 7072
SalI G'TCGA_C
Cuts at: 1936 4414 1936
Size: 2478 4594
SanDI GG'GwC_CC
Cuts at: 3475 3475
Size: 7072
SapI GCTCTTCn'nnn_
Cuts at: 3675 4268 3675
Size: 593 6479
SbfI CC_TGCA'GG
Cuts at: 4424 4424
Size: 7072
ScaI AGT'ACT
Cuts at: 5320 7032 5320
Size: 1712 5360
SfcI C'TryA_G
Cuts at: 826 1045 3039 4291 4420 5555 6236 6427 826
Size: 219 1994 1252 129 1135 681 191 1471
Fragments arranged by size:
1994 1471 1252 1135 681 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7072
SmaI CCC'GGG
Cuts at: 1872 4399 1872
Size: 2527 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5176 6077 6339 820
Size: 197 926 3233 901 262 1553
Fragments arranged by size:
3233 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4863 493
Size: 4370 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7072
SphI G_CATG'C
Cuts at: 1132 4430 1132
Size: 3298 3774
SspI AAT'ATT
Cuts at: 5 52 3518 4996 5
Size: 47 3466 1478 2081
Fragments arranged by size:
3466 2081 1478 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7072
StyI C'CwwG_G
Cuts at: 513 1065 2545 2772 3470 4057 4511 513
Size: 552 1480 227 698 587 454 3074
Fragments arranged by size:
3074 1480 698 587 552 454 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3669 5099 5116 5269 5454 5899 325
Size: 1255 2089 1430 17 153 185 445 1498
Fragments arranged by size:
2089 1498 1430 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3339 3407 5318
Size: 274 80 33 51 1443 1362 68 1911
Cuts at: 5318 7030 96
Size: 1712 138
Fragments arranged by size:
1911 1712 1443 1362 274 138 80 68
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7032 7064 7032
Size: 32 7040
Tth111I GACn'n_nGTC
Cuts at: 1316 2516 1316
Size: 1200 5872
XbaI T'CTAG_A
Cuts at: 3116 4408 3116
Size: 1292 5780
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2073 2191 2241 2329 3646 2011
Size: 62 118 50 88 1317 5437
Fragments arranged by size:
5437 1317 118 88 62 50
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7072
XmaI C'CCGG_G
Cuts at: 1870 4397 1870
Size: 2527 4545
XmnI GAAnn'nnTTC
Cuts at: 5201 5201
Size: 7072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4880 276
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
RsrII SexAI SfiI SfoI SrfI SwaI