Restriction map for
pFN21AA1477
(Circular) (Six-base) MAPSORT of: pFN21AA1477.seq Check: 9370 from: 1 to: 6979
pFN21AA1477 6979 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3838 3838
Size: 6979
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4789 278
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2305 4322 1937
Size: 368 2017 4594
Fragments arranged by size:
4594 2017 368
Acc65I G'GTAC_C
Cuts at: 4300 4300
Size: 6979
AclI AA'CG_TT
Cuts at: 1563 5106 5479 1563
Size: 3543 373 3063
Fragments arranged by size:
3543 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2667 2822 3125 3179 3678 5042 6426 6694
Size: 1035 155 303 54 499 1364 1384 268
Cuts at: 6694 1632
Size: 1917
Fragments arranged by size:
1917 1384 1364 1035 499 303 268 155
54
AflII C'TTAA_G
Cuts at: 820 1017 2486 820
Size: 197 1469 5313
Fragments arranged by size:
5313 1469 197
AflIII A'CryG_T
Cuts at: 1203 2239 5878 6855 1203
Size: 1036 3639 977 1327
Fragments arranged by size:
3639 1327 1036 977
AgeI A'CCGG_T
Cuts at: 5810 5810
Size: 6979
AhdI GACnn_n'nnGTC
Cuts at: 5708 5708
Size: 6979
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6979
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2073 2105 2073
Size: 32 6947
AlwNI CAG_nnn'CTG
Cuts at: 4722 6294 4722
Size: 1572 5407
ApaLI G'TGCA_C
Cuts at: 5036 6192 5036
Size: 1156 5823
ApoI r'AATT_y
Cuts at: 1493 2212 2423 2555 2719 3059 3914 4288 4514
Size: 719 211 132 164 340 855 374 226
Cuts at: 4514 4666 6775 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 855 719 374 340 226 211
164 152 132
AseI AT'TA_AT
Cuts at: 160 1032 5533 160
Size: 872 4501 1606
Fragments arranged by size:
4501 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
AvaI C'yCGr_G
Cuts at: 1870 1943 2612 3753 4304 1870
Size: 73 669 1141 551 4545
Fragments arranged by size:
4545 1141 669 551 73
BamHI G'GATC_C
Cuts at: 4309 4676 4309
Size: 367 6612
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4300 5755 618
Size: 325 217 627 2513 1455 1842
Fragments arranged by size:
2513 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3764 4298 1391
Size: 2373 534 4072
Fragments arranged by size:
4072 2373 534
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4019 928
Size: 971 2120 3888
Fragments arranged by size:
3888 2120 971
BbvCI CC'TCA_GC
Cuts at: 3574 3574
Size: 6979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3338 3372 5170 5204 1903
Size: 34 1401 34 1798 34 3678
Fragments arranged by size:
3678 1798 1401 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4871 6081 4871
Size: 1210 5769
BclI T'GATC_A
Cuts at: 1548 3208 1548
Size: 1660 5319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5590 136
Size: 107 122 71 631 605 337 3581 1525
Fragments arranged by size:
3581 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6974 6974
Size: 6979
BlpI GC'TnA_GC
Cuts at: 4396 4396
Size: 6979
Bme1580I G_kGCm'C
Cuts at: 1790 5040 6196 1790
Size: 3250 1156 2573
Fragments arranged by size:
3250 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6979
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4710 5668
Size: 416 53 83 399 24 1691 1602 958
Cuts at: 5668 442
Size: 1753
Fragments arranged by size:
1753 1691 1602 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3444 3717 5639 1239
Size: 2205 273 1922 2579
Fragments arranged by size:
2579 2205 1922 273
Bpu10I CC'TnA_GC
Cuts at: 3574 3574
Size: 6979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 5104 5969 6267 3748
Size: 1356 865 298 4460
Fragments arranged by size:
4460 1356 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5642 882
Size: 617 4143 2219
Fragments arranged by size:
4143 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3481 4770 493
Size: 711 2277 1289 2702
Fragments arranged by size:
2702 2277 1289 711
BsaBI GATnn'nnATC
Cuts at: 6973 6973
Size: 6979
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2670 4786 5168
Size: 53 83 186 936 485 652 2116 382
Cuts at: 5168 5803 275
Size: 635 1451
Fragments arranged by size:
2116 1451 936 652 635 485 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2920 3738 5412 5810 6084 6231 6642 2920
Size: 818 1674 398 274 147 411 3257
Fragments arranged by size:
3257 1674 818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3715 3745 1178
Size: 30 165 30 2312 30 4412
Fragments arranged by size:
4412 2312 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3305 1186
Size: 2119 4860
BseYI C'CCAG_C
Cuts at: 6182 6678 6182
Size: 496 6483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4172 1163
Size: 573 2436 3970
Fragments arranged by size:
3970 2436 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4429 5190 6218 1540
Size: 275 186 2428 761 1028 2301
Fragments arranged by size:
2428 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3764 5040 5125 6196 3764
Size: 1276 85 1071 4547
Fragments arranged by size:
4547 1276 1071 85
BsmI GAATG_Cn'
Cuts at: 4490 4583 4490
Size: 93 6886
BsmBI CGTCTCn'nnnn_
Cuts at: 4122 4122
Size: 6979
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3764 4298 5040 5125 6196 1391
Size: 399 1974 534 742 85 1071 2174
Fragments arranged by size:
2174 1974 1071 742 534 399 85
BspEI T'CCGG_A
Cuts at: 2920 6642 2920
Size: 3722 3257
BspHI T'CATG_A
Cuts at: 2881 4868 6598 2881
Size: 1987 1730 3262
Fragments arranged by size:
3262 1987 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3838 4334 844
Size: 2994 496 3489
Fragments arranged by size:
3489 2994 496
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 4866 1394
Size: 429 218 2825 3507
Fragments arranged by size:
3507 2825 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 2021 3485 4245 5474 5648 65
Size: 1183 773 1464 760 1229 174 1396
Fragments arranged by size:
1464 1396 1229 1183 773 760 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3666 5623 5810 1700
Size: 110 1856 1957 187 2869
Fragments arranged by size:
2869 1957 1856 187 110
BsrGI T'GTAC_A
Cuts at: 96 2446 96
Size: 2350 4629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6979
BssSI C'ACGA_G
Cuts at: 2973 4148 5039 6051 2973
Size: 1175 891 1012 3901
Fragments arranged by size:
3901 1175 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6979
BstBI TT'CG_AA
Cuts at: 5783 5783
Size: 6979
BstEII G'GTnAC_C
Cuts at: 1184 2956 1184
Size: 1772 5207
BstYI r'GATC_y
Cuts at: 1972 4309 4676 5061 5078 6530 6616 6628 6974
Size: 2337 367 385 17 1452 86 12 346
Cuts at: 6974 1972
Size: 1977
Fragments arranged by size:
2337 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3258 3258
Size: 6979
BtgI C'CryG_G
Cuts at: 513 1065 2389 2961 3781 4123 513
Size: 552 1324 572 820 342 3369
Fragments arranged by size:
3369 1324 820 572 552 342
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6240
Fragments arranged by size:
6240 683 56
BtsI GCAGTG_nn'
Cuts at: 3632 4160 4495 5289 5309 3632
Size: 528 335 794 20 5302
Fragments arranged by size:
5302 794 528 335 20
ClaI AT'CG_AT
Cuts at: 1554 2863 3475 1554
Size: 1309 612 5058
Fragments arranged by size:
5058 1309 612
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6944
DraI TTT'AAA
Cuts at: 2724 4283 2724
Size: 1559 5420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2954 5986 809
Size: 507 1638 3032 1802
Fragments arranged by size:
3032 1802 1638 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2713 4426 5315
Size: 54 1425 50 275 18 883 1713 889
Cuts at: 5315 6944 8
Size: 1629 43
Fragments arranged by size:
1713 1629 1425 889 883 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4426 1537
Size: 275 2614 4090
Fragments arranged by size:
4090 2614 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2880 3144 3556 3957 4909 1353
Size: 1527 264 412 401 952 3423
Fragments arranged by size:
3423 1527 952 412 401 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5566 5940 6086 387
Size: 1064 138 3977 374 146 1280
Fragments arranged by size:
3977 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3762 3762
Size: 6979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2667 2822 3125 3179 3444 3678 3717
Size: 393 1035 155 303 54 265 234 39
Cuts at: 3717 5042 5639 6426 6694 1239
Size: 1325 597 787 268 1524
Fragments arranged by size:
1524 1325 1035 787 597 393 303 268
265 234 155 54 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2358 3369 2358
Size: 1011 5968
EcoO109I rG'GnC_Cy
Cuts at: 3105 3105
Size: 6979
EcoRI G'AATT_C
Cuts at: 4288 4666 4288
Size: 378 6601
FspI TGC'GCA
Cuts at: 5485 5485
Size: 6979
HaeII r_GCGC'y
Cuts at: 1678 6126 1678
Size: 4448 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2332 2364 3457
Size: 32 135 32 534 32 194 32 1093
Cuts at: 3457 3489 5620 5652 5694 5726 1373
Size: 32 2131 32 42 32 2626
Fragments arranged by size:
2626 2131 1093 534 194 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3885 4323 4569 669
Size: 988 281 1947 438 246 3079
Fragments arranged by size:
3079 1947 988 438 281 246
HindIII A'AGCT_T
Cuts at: 748 3660 748
Size: 2912 4067
HpaI GTT'AAC
Cuts at: 4569 4569
Size: 6979
KpnI G_GTAC'C
Cuts at: 4304 4304
Size: 6979
MfeI C'AATT_G
Cuts at: 4578 4578
Size: 6979
MluI A'CGCG_T
Cuts at: 6855 6855
Size: 6979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3316 6093 6277 1305
Size: 2011 2777 184 2007
Fragments arranged by size:
2777 2011 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2715 10
Size: 54 1425 343 883 4274
Fragments arranged by size:
4274 1425 883 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4937 5296 5455 518
Size: 648 3771 359 159 2042
Fragments arranged by size:
3771 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3196 4395 5072 6220 6465 1606
Size: 1590 1199 677 1148 245 2120
Fragments arranged by size:
2120 1590 1199 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2389 2961 3781 4123 513
Size: 552 1324 572 820 342 3369
Fragments arranged by size:
3369 1324 820 572 552 342
NdeI CA'TA_TG
Cuts at: 387 3451 387
Size: 3064 3915
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6979
NotI GC'GGCC_GC
Cuts at: 4426 4426
Size: 6979
NspI r_CATG'y
Cuts at: 1132 1229 3869 4337 5882 1132
Size: 97 2640 468 1545 2229
Fragments arranged by size:
2640 2229 1545 468 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6979
PciI A'CATG_T
Cuts at: 5878 5878
Size: 6979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6979
PfoI T'CCnGG_A
Cuts at: 3795 3795
Size: 6979
PmeI GTTT'AAAC
Cuts at: 4283 4283
Size: 6979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5048 5080 6561 6593 5048
Size: 32 1481 32 5434
Fragments arranged by size:
5434 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3105 3105
Size: 6979
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6979
PsiI TTA'TAA
Cuts at: 3819 4549 3819
Size: 730 6249
PstI C_TGCA'G
Cuts at: 830 3547 4331 830
Size: 2717 784 3478
Fragments arranged by size:
3478 2717 784
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6979
SacI G_AGCT'C
Cuts at: 3764 3764
Size: 6979
SalI G'TCGA_C
Cuts at: 1936 4321 1936
Size: 2385 4594
SapI GCTCTTCn'nnn_
Cuts at: 3556 3556
Size: 6979
SbfI CC_TGCA'GG
Cuts at: 4331 4331
Size: 6979
ScaI AGT'ACT
Cuts at: 3724 5227 6939 3724
Size: 1503 1712 3764
Fragments arranged by size:
3764 1712 1503
SfcI C'TryA_G
Cuts at: 826 1045 3543 4327 5462 6143 6334 826
Size: 219 2498 784 1135 681 191 1471
Fragments arranged by size:
2498 1471 1135 784 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6979
SmaI CCC'GGG
Cuts at: 1872 4306 1872
Size: 2434 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3763 5083 5984 6246 820
Size: 197 926 543 1277 1320 901 262 1553
Fragments arranged by size:
1553 1320 1277 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 3481 4770 493
Size: 2988 1289 2702
Fragments arranged by size:
2988 2702 1289
SpeI A'CTAG_T
Cuts at: 152 4019 152
Size: 3867 3112
SphI G_CATG'C
Cuts at: 1132 4337 1132
Size: 3205 3774
SspI AAT'ATT
Cuts at: 5 52 2524 4903 5
Size: 47 2472 2379 2081
Fragments arranged by size:
2472 2379 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6979
StyI C'CwwG_G
Cuts at: 513 1065 2389 2623 2961 3781 4123 4418 513
Size: 552 1324 234 338 820 342 295 3074
Fragments arranged by size:
3074 1324 820 552 342 338 295 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5006 5023 5176 5361 5806 325
Size: 1255 3426 17 153 185 445 1498
Fragments arranged by size:
3426 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2446 2466 3722
Size: 274 80 33 51 1443 469 20 1256
Cuts at: 3722 3929 5225 6937 96
Size: 207 1296 1712 138
Fragments arranged by size:
1712 1443 1296 1256 469 274 207 138
80 51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 6939 6971 3581
Size: 32 3326 32 3589
Fragments arranged by size:
3589 3326 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6979
XbaI T'CTAG_A
Cuts at: 4315 4315
Size: 6979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 4024 2227
Size: 1797 5182
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6979
XmaI C'CCGG_G
Cuts at: 1870 4304 1870
Size: 2434 4545
XmnI GAAnn'nnTTC
Cuts at: 2499 2796 5108 2499
Size: 297 2312 4370
Fragments arranged by size:
4370 2312 297
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4787 276
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI