Restriction map for
pFN21AA1475
(Circular) (Six-base) MAPSORT of: pFN21AA1475.seq Check: 6088 from: 1 to: 7624
pFN21AA1475 7624 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2346 3049 3916 4189 4577 2346
Size: 703 867 273 388 5393
Fragments arranged by size:
5393 867 703 388 273
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5434 278
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4152 4967 1937
Size: 2215 815 4594
Fragments arranged by size:
4594 2215 815
Acc65I G'GTAC_C
Cuts at: 4945 4945
Size: 7624
AclI AA'CG_TT
Cuts at: 1563 5751 6124 1563
Size: 4188 373 3063
Fragments arranged by size:
4188 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3122 4295 5687 7071 7339 1632
Size: 1490 1173 1392 1384 268 1917
Fragments arranged by size:
1917 1490 1392 1384 1173 268
AfeI AGC'GCT
Cuts at: 4895 4895
Size: 7624
AflII C'TTAA_G
Cuts at: 820 1017 4430 820
Size: 197 3413 4014
Fragments arranged by size:
4014 3413 197
AflIII A'CryG_T
Cuts at: 1203 6523 7500 1203
Size: 5320 977 1327
Fragments arranged by size:
5320 1327 977
AgeI A'CCGG_T
Cuts at: 6455 6455
Size: 7624
AhdI GACnn_n'nnGTC
Cuts at: 6353 6353
Size: 7624
AleI CACnn'nnGTG
Cuts at: 1166 2617 1166
Size: 1451 6173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2635 1184
Size: 1451 6173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 2024
Size: 32 7592
AlwNI CAG_nnn'CTG
Cuts at: 2615 4459 4522 5367 6939 2615
Size: 1844 63 845 1572 3300
Fragments arranged by size:
3300 1844 1572 845 63
ApaI G_GGCC'C
Cuts at: 2645 4785 2645
Size: 2140 5484
ApaLI G'TGCA_C
Cuts at: 3043 5681 6837 3043
Size: 2638 1156 3830
Fragments arranged by size:
3830 2638 1156
ApoI r'AATT_y
Cuts at: 1493 3089 4933 5159 5311 7420 1493
Size: 1596 1844 226 152 2109 1697
Fragments arranged by size:
2109 1844 1697 1596 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6178 160
Size: 872 5146 1606
Fragments arranged by size:
5146 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2847 3189 3285 4743 4949 1870
Size: 73 149 755 342 96 1458 206 4545
Fragments arranged by size:
4545 1458 755 342 206 149 96 73
BamHI G'GATC_C
Cuts at: 4954 5321 4954
Size: 367 7257
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2904 4945 6400 618
Size: 325 217 627 1117 2041 1455 1842
Fragments arranged by size:
2041 1842 1455 1117 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2265 2526 2645 2745 3498 3520 4551 4739
Size: 874 261 119 100 753 22 1031 188
Cuts at: 4739 4785 4943 1391
Size: 46 158 4072
Fragments arranged by size:
4072 1031 874 753 261 188 158 119
100 46 22
BbeI G_GCGC'C
Cuts at: 2908 2908
Size: 7624
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3154 928
Size: 971 1255 5398
Fragments arranged by size:
5398 1255 971
BbvCI CC'TCA_GC
Cuts at: 2721 3584 2721
Size: 863 6761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5815 5849 1903
Size: 34 3878 34 3678
Fragments arranged by size:
3878 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2218 5516 6726 2218
Size: 3298 1210 3116
Fragments arranged by size:
3298 3116 1210
BclI T'GATC_A
Cuts at: 1548 3687 1548
Size: 2139 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3290 3623 4536
Size: 107 122 71 631 605 1618 333 913
Cuts at: 4536 4754 6235 136
Size: 218 1481 1525
Fragments arranged by size:
1618 1525 1481 913 631 605 333 218
122 107 71
BglII A'GATC_T
Cuts at: 7619 7619
Size: 7624
BlpI GC'TnA_GC
Cuts at: 2921 5041 2921
Size: 2120 5504
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2462 2645 3047 4495 4785 5685 6841
Size: 340 332 183 402 1448 290 900 1156
Cuts at: 6841 1790
Size: 2573
Fragments arranged by size:
2573 1448 1156 900 402 340 332 290
183
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 2992 5355
Size: 416 53 83 399 24 1048 527 2363
Cuts at: 5355 6313 442
Size: 958 1753
Fragments arranged by size:
2363 1753 1048 958 527 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4341 1056
Size: 3285 4339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2176 3104 3344 3407 4073 4709 6284
Size: 896 41 928 240 63 666 636 1575
Cuts at: 6284 1239
Size: 2579
Fragments arranged by size:
2579 1575 928 896 666 636 240 63
41
Bpu10I CC'TnA_GC
Cuts at: 2351 2721 3503 3584 2351
Size: 370 782 81 6391
Fragments arranged by size:
6391 782 370 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2648 3362 4079 4337 4784 5749 6614 6912 2648
Size: 714 717 258 447 965 865 298 3360
Fragments arranged by size:
3360 965 865 717 714 447 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4503 6287 882
Size: 617 3004 1784 2219
Fragments arranged by size:
3004 2219 1784 617
BsaAI yAC'GTr
Cuts at: 493 1204 2272 2374 2587 3175 4291 5415 493
Size: 711 1068 102 213 588 1116 1124 2702
Fragments arranged by size:
2702 1124 1116 1068 711 588 213 102
BsaBI GATnn'nnATC
Cuts at: 7618 7618
Size: 7624
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2905 5431 5813
Size: 53 83 186 936 476 896 2526 382
Cuts at: 5813 6448 275
Size: 635 1451
Fragments arranged by size:
2526 1451 936 896 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2560 6057 6455 6729 6876 7287 2560
Size: 3497 398 274 147 411 2897
Fragments arranged by size:
3497 2897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3026 3056 1178
Size: 30 165 30 1623 30 5746
Fragments arranged by size:
5746 1623 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2942 3112 3380 3416 3758 4190 4256 4706
Size: 1756 170 268 36 342 432 66 450
Cuts at: 4706 4916 4919 1186
Size: 210 3 3891
Fragments arranged by size:
3891 1756 450 432 342 268 210 170
66 36 3
BseYI C'CCAG_C
Cuts at: 2109 2278 2789 3780 3970 4061 4494 4708 4726
Size: 169 511 991 190 91 433 214 18
Cuts at: 4726 4784 4890 6827 7323 2109
Size: 58 106 1937 496 2410
Fragments arranged by size:
2410 1937 991 511 496 433 214 190
169 106 91 58 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4248 4316 1163
Size: 573 2512 68 4471
Fragments arranged by size:
4471 2512 573 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 4031 4873 4877 5074 5835
Size: 275 186 51 1979 842 4 197 761
Cuts at: 5835 6863 1540
Size: 1028 2301
Fragments arranged by size:
2301 1979 1028 842 761 275 197 186
51 4
BsiHKAI G_wGCw'C
Cuts at: 2526 2624 3047 3498 3762 5685 5770 6841 2526
Size: 98 423 451 264 1923 85 1071 3309
Fragments arranged by size:
3309 1923 1071 451 423 264 98 85
BsmI GAATG_Cn'
Cuts at: 2110 3572 5135 5228 2110
Size: 1462 1563 93 4506
Fragments arranged by size:
4506 1563 1462 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2194 2194
Size: 7624
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2265 2462 2526 2624 2645 2745
Size: 399 340 135 197 64 98 21 100
Cuts at: 2745 3047 3498 3520 3762 4495 4551 4739 4785
Size: 302 451 22 242 733 56 188 46
Cuts at: 4785 4943 5685 5770 6841 1391
Size: 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 451 399 340 302
242 197 188 158 135 100 98 85
64 56 46 22 21
BspEI T'CCGG_A
Cuts at: 2560 7287 2560
Size: 4727 2897
BspHI T'CATG_A
Cuts at: 5513 7243 5513
Size: 1730 5894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 3049 3916 4011 4189 4242 4521 4577
Size: 1502 703 867 95 178 53 279 56
Cuts at: 4577 4979 844
Size: 402 3489
Fragments arranged by size:
3489 1502 867 703 402 279 178 95
56 53
BsrBI CCG'CTC
Cuts at: 1394 1823 2046 2638 3154 3256 3706 4863 5511
Size: 429 223 592 516 102 450 1157 648
Cuts at: 5511 1394
Size: 3507
Fragments arranged by size:
3507 1157 648 592 516 450 429 223
102
BsrDI GCAATG_nn'
Cuts at: 65 1248 6119 6293 65
Size: 1183 4871 174 1396
Fragments arranged by size:
4871 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3507 3861 4026 4872 6268 6455 1700
Size: 110 1697 354 165 846 1396 187 2869
Fragments arranged by size:
2869 1697 1396 846 354 187 165 110
BsrGI T'GTAC_A
Cuts at: 96 4312 96
Size: 4216 3408
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7624
BssSI C'ACGA_G
Cuts at: 3731 5684 6696 3731
Size: 1953 1012 4659
Fragments arranged by size:
4659 1953 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3064 4294 4522 4651 794
Size: 2270 1230 228 129 3767
Fragments arranged by size:
3767 2270 1230 228 129
BstBI TT'CG_AA
Cuts at: 3574 6428 3574
Size: 2854 4770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7624
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2797 2272
Size: 525 7099
BstYI r'GATC_y
Cuts at: 1972 2563 4954 5321 5706 5723 7175 7261 7273
Size: 591 2391 367 385 17 1452 86 12
Cuts at: 7273 7619 1972
Size: 346 1977
Fragments arranged by size:
2391 1977 1452 591 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4239 4239
Size: 7624
BtgI C'CryG_G
Cuts at: 513 1065 2004 4021 4182 4323 4773 513
Size: 552 939 2017 161 141 450 3364
Fragments arranged by size:
3364 2017 939 552 450 161 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6885
Fragments arranged by size:
6885 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 3045 3470 3690 4229 5140 5934 5954 2228
Size: 817 425 220 539 911 794 20 3898
Fragments arranged by size:
3898 911 817 794 539 425 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4121 4156 529
Size: 35 3557 35 3997
Fragments arranged by size:
3997 3557 35 35
DraI TTT'AAA
Cuts at: 4928 4928
Size: 7624
DraIII CAC_nnn'GTG
Cuts at: 3112 3673 4081 4294 3112
Size: 561 408 213 6442
Fragments arranged by size:
6442 561 408 213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6631 809
Size: 507 5315 1802
Fragments arranged by size:
5315 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2399 3234
Size: 54 1425 50 275 18 219 350 835
Cuts at: 3234 3435 3753 4024 4028 4126 4215 4615 4824
Size: 201 318 271 4 98 89 400 209
Cuts at: 4824 4874 5071 5960 7589 8
Size: 50 197 889 1629 43
Fragments arranged by size:
1629 1425 889 835 400 350 318 275
271 219 209 201 197 98 89 54
50 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2049 4028 4874 5071 1537
Size: 275 237 1979 846 197 4090
Fragments arranged by size:
4090 1979 846 275 237 197
EarI CTCTTCn'nnn_
Cuts at: 1353 2693 3791 4296 4470 4610 5554 1353
Size: 1340 1098 505 174 140 944 3423
Fragments arranged by size:
3423 1340 1098 944 505 174 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2564 6211 6585 6731 387
Size: 1064 138 975 3647 374 146 1280
Fragments arranged by size:
3647 1280 1064 975 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 3496 2524
Size: 972 6652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2176 3104 3122 3344 3407 4073
Size: 393 503 41 928 18 222 63 666
Cuts at: 4073 4295 4709 5687 6284 7071 7339 1239
Size: 222 414 978 597 787 268 1524
Fragments arranged by size:
1524 978 928 787 666 597 503 414
393 268 222 222 63 41 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 4238 4643 2219
Size: 2019 405 5200
Fragments arranged by size:
5200 2019 405
EcoO109I rG'GnC_Cy
Cuts at: 2365 2477 2641 2642 2960 3538 3748 4159 4781
Size: 112 164 1 318 578 210 411 622
Cuts at: 4781 2365
Size: 5208
Fragments arranged by size:
5208 622 578 411 318 210 164 112
1
EcoRI G'AATT_C
Cuts at: 4933 5311 4933
Size: 378 7246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2561 3586 2561
Size: 1025 6599
FseI GG_CCGG'CC
Cuts at: 4030 4030
Size: 7624
FspI TGC'GCA
Cuts at: 4259 6130 4259
Size: 1871 5753
HaeII r_GCGC'y
Cuts at: 1678 2686 2908 4897 6771 1678
Size: 1008 222 1989 1874 2531
Fragments arranged by size:
2531 1989 1874 1008 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6265 6297 6339 6371 1373
Size: 32 135 32 4693 32 42 32 2626
Fragments arranged by size:
4693 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4968 5214 669
Size: 988 281 3030 246 3079
Fragments arranged by size:
3079 3030 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7624
HpaI GTT'AAC
Cuts at: 5214 5214
Size: 7624
KasI G'GCGC_C
Cuts at: 2904 2904
Size: 7624
KpnI G_GTAC'C
Cuts at: 4949 4949
Size: 7624
MfeI C'AATT_G
Cuts at: 5223 5223
Size: 7624
MluI A'CGCG_T
Cuts at: 7500 7500
Size: 7624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2423 6738 6922 1305
Size: 1118 4315 184 2007
Fragments arranged by size:
4315 2007 1118 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3236 3437 4128 4217 4617
Size: 54 1425 343 1404 201 691 89 400
Cuts at: 4617 10
Size: 3017
Fragments arranged by size:
3017 1425 1404 691 400 343 201 89
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2617 5582 5941 6100 518
Size: 648 1451 2965 359 159 2042
Fragments arranged by size:
2965 2042 1451 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2203 2425 3100 3136 4465 4829 5040
Size: 507 90 222 675 36 1329 364 211
Cuts at: 5040 5717 6865 7110 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1329 1148 677 675 507 364 245
222 211 90 36
NaeI GCC'GGC
Cuts at: 3509 3863 4028 3509
Size: 354 165 7105
Fragments arranged by size:
7105 354 165
NarI GG'CG_CC
Cuts at: 2905 2905
Size: 7624
NcoI C'CATG_G
Cuts at: 513 1065 2004 4323 513
Size: 552 939 2319 3814
Fragments arranged by size:
3814 2319 939 552
NdeI CA'TA_TG
Cuts at: 387 4620 387
Size: 4233 3391
NgoMIV G'CCGG_C
Cuts at: 3507 3861 4026 3507
Size: 354 165 7105
Fragments arranged by size:
7105 354 165
NheI G'CTAG_C
Cuts at: 1052 4337 1052
Size: 3285 4339
NotI GC'GGCC_GC
Cuts at: 5071 5071
Size: 7624
NspI r_CATG'y
Cuts at: 1132 1229 4982 6527 1132
Size: 97 3753 1545 2229
Fragments arranged by size:
3753 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2728 1348
Size: 1380 6244
PciI A'CATG_T
Cuts at: 6523 6523
Size: 7624
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7624
PfoI T'CCnGG_A
Cuts at: 2016 3081 3384 4452 2016
Size: 1065 303 1068 5188
Fragments arranged by size:
5188 1068 1065 303
PmeI GTTT'AAAC
Cuts at: 4928 4928
Size: 7624
PmlI CAC'GTG
Cuts at: 2272 3175 4291 2272
Size: 903 1116 5605
Fragments arranged by size:
5605 1116 903
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5693 5725 7206 7238 5693
Size: 32 1481 32 6079
Fragments arranged by size:
6079 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 4159 2365
Size: 1794 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7624
PsiI TTA'TAA
Cuts at: 5194 5194
Size: 7624
PspOMI G'GGCC_C
Cuts at: 2641 4781 2641
Size: 2140 5484
PstI C_TGCA'G
Cuts at: 830 3426 3525 4200 4424 4464 4518 4647 4701
Size: 2596 99 675 224 40 54 129 54
Cuts at: 4701 4976 830
Size: 275 3478
Fragments arranged by size:
3478 2596 675 275 224 129 99 54
54 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7624
PvuII CAG'CTG
Cuts at: 2113 2203 2425 3136 2113
Size: 90 222 711 6601
Fragments arranged by size:
6601 711 222 90
SacI G_AGCT'C
Cuts at: 2526 3498 2526
Size: 972 6652
SalI G'TCGA_C
Cuts at: 1936 4966 1936
Size: 3030 4594
SapI GCTCTTCn'nnn_
Cuts at: 3791 3791
Size: 7624
SbfI CC_TGCA'GG
Cuts at: 4200 4518 4976 4200
Size: 318 458 6848
Fragments arranged by size:
6848 458 318
ScaI AGT'ACT
Cuts at: 5872 7584 5872
Size: 1712 5912
SfcI C'TryA_G
Cuts at: 826 1045 2803 3422 3521 3901 4196 4420 4460
Size: 219 1758 619 99 380 295 224 40
Cuts at: 4460 4482 4514 4643 4697 4972 6107 6788 6979
Size: 22 32 129 54 275 1135 681 191
Cuts at: 6979 826
Size: 1471
Fragments arranged by size:
1758 1471 1135 681 619 380 295 275
224 219 191 129 99 54 40 32
22
SfiI GGCCn_nnn'nGGCC
Cuts at: 3290 4754 3290
Size: 1464 6160
SfoI GGC'GCC
Cuts at: 2906 2906
Size: 7624
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7624
SmaI CCC'GGG
Cuts at: 1872 2849 3287 4745 4951 1872
Size: 977 438 1458 206 4545
Fragments arranged by size:
4545 1458 977 438 206
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 3377 4094 4352 4430 4799
Size: 197 926 684 750 717 258 78 369
Cuts at: 4799 5728 6629 6891 820
Size: 929 901 262 1553
Fragments arranged by size:
1553 929 926 901 750 717 684 369
262 258 197 78
SnaBI TAC'GTA
Cuts at: 493 5415 493
Size: 4922 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7624
SphI G_CATG'C
Cuts at: 1132 4982 1132
Size: 3850 3774
SrfI GCCC'GGGC
Cuts at: 4745 4745
Size: 7624
SspI AAT'ATT
Cuts at: 5 52 5548 5
Size: 47 5496 2081
Fragments arranged by size:
5496 2081 47
StuI AGG'CCT
Cuts at: 1517 3737 4436 1517
Size: 2220 699 4705
Fragments arranged by size:
4705 2220 699
StyI C'CwwG_G
Cuts at: 513 1065 2004 2500 3561 4323 4731 4749 5063
Size: 552 939 496 1061 762 408 18 314
Cuts at: 5063 513
Size: 3074
Fragments arranged by size:
3074 1061 939 762 552 496 408 314
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2781 3817 5651 5668 5821 6006 6451
Size: 1255 1201 1036 1834 17 153 185 445
Cuts at: 6451 325
Size: 1498
Fragments arranged by size:
1834 1498 1255 1201 1036 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3675 4312 4839
Size: 274 80 33 51 1443 1698 637 527
Cuts at: 4839 5870 7582 96
Size: 1031 1712 138
Fragments arranged by size:
1712 1698 1443 1031 637 527 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2760 2792 3026 3058 7584 7616 2760
Size: 32 234 32 4526 32 2768
Fragments arranged by size:
4526 2768 234 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3275 1316
Size: 1959 5665
XbaI T'CTAG_A
Cuts at: 4960 4960
Size: 7624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2409 3946 4330 2409
Size: 1537 384 5703
Fragments arranged by size:
5703 1537 384
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7624
XmaI C'CCGG_G
Cuts at: 1870 2847 3285 4743 4949 1870
Size: 977 438 1458 206 4545
Fragments arranged by size:
4545 1458 977 438 206
XmnI GAAnn'nnTTC
Cuts at: 3224 3570 5753 3224
Size: 346 2183 5095
Fragments arranged by size:
5095 2183 346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5432 276
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV
FspAI NruI NsiI PacI PspXI PsrI RsrII SacII
SanDI SexAI SwaI