Restriction map for
pFN21AA1461
(Circular) (Six-base) MAPSORT of: pFN21AA1461.seq Check: 7140 from: 1 to: 9187
pFN21AA1461 9187 bp
With 182 enzymes: SgfI *
February 26, 2010 09:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6997 278
Size: 53 83 186 936 5461 2468
Fragments arranged by size:
5461 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2595 6530 1937
Size: 658 3935 4594
Fragments arranged by size:
4594 3935 658
Acc65I G'GTAC_C
Cuts at: 3169 4968 6508 3169
Size: 1799 1540 5848
Fragments arranged by size:
5848 1799 1540
AclI AA'CG_TT
Cuts at: 1563 7314 7687 1563
Size: 5751 373 3063
Fragments arranged by size:
5751 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3026 3867 4235 4536 4821 6045 7250
Size: 1185 209 841 368 301 285 1224 1205
Cuts at: 7250 8634 8902 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1224 1205 1185 841 368 301
285 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8990
AflIII A'CryG_T
Cuts at: 1203 8086 9063 1203
Size: 6883 977 1327
Fragments arranged by size:
6883 1327 977
AgeI A'CCGG_T
Cuts at: 8018 8018
Size: 9187
AhdI GACnn_n'nnGTC
Cuts at: 6406 7916 6406
Size: 1510 7677
AleI CACnn'nnGTG
Cuts at: 1166 4469 1166
Size: 3303 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3732 5091 5318 1184
Size: 2548 1359 227 5053
Fragments arranged by size:
5053 2548 1359 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6092 6124 6092
Size: 32 9155
AlwNI CAG_nnn'CTG
Cuts at: 4909 6930 8502 4909
Size: 2021 1572 5594
Fragments arranged by size:
5594 2021 1572
ApaLI G'TGCA_C
Cuts at: 3332 4280 5865 7244 8400 3332
Size: 948 1585 1379 1156 4119
Fragments arranged by size:
4119 1585 1379 1156 948
ApoI r'AATT_y
Cuts at: 1493 2476 3415 4417 4449 5482 5645 5778 5791
Size: 983 939 1002 32 1033 163 133 13
Cuts at: 5791 5983 6496 6722 6874 8983 1493
Size: 192 513 226 152 2109 1697
Fragments arranged by size:
2109 1697 1033 1002 983 939 513 226
192 163 152 133 32 13
AseI AT'TA_AT
Cuts at: 160 1032 3132 3578 7741 160
Size: 872 2100 446 4163 1606
Fragments arranged by size:
4163 2100 1606 872 446
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9187
AvaI C'yCGr_G
Cuts at: 1870 1943 2424 4165 6254 6275 6512 1870
Size: 73 481 1741 2089 21 237 4545
Fragments arranged by size:
4545 2089 1741 481 237 73 21
BamHI G'GATC_C
Cuts at: 6517 6884 6517
Size: 367 8820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4559 4968 6508 7963
Size: 325 217 627 1382 1390 409 1540 1455
Cuts at: 7963 618
Size: 1842
Fragments arranged by size:
1842 1540 1455 1390 1382 627 409 325
217
BanII G_rGCy'C
Cuts at: 1391 2785 5118 5497 6276 6506 1391
Size: 1394 2333 379 779 230 4072
Fragments arranged by size:
4072 2333 1394 779 379 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 6228 6293 928
Size: 971 143 4186 65 3822
Fragments arranged by size:
4186 3822 971 143 65
BbvCI CC'TCA_GC
Cuts at: 5477 5477
Size: 9187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3905 3939 7378 7412 1903
Size: 34 1968 34 3439 34 3678
Fragments arranged by size:
3678 3439 1968 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7079 8289 7079
Size: 1210 7977
BclI T'GATC_A
Cuts at: 1548 5461 1548
Size: 3913 5274
BfrBI ATG'CAT
Cuts at: 2304 2304
Size: 9187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2661 7798 136
Size: 107 122 71 631 605 989 5137 1525
Fragments arranged by size:
5137 1525 989 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3696 6477 9182 3696
Size: 2781 2705 3701
Fragments arranged by size:
3701 2781 2705
BlpI GC'TnA_GC
Cuts at: 5918 6604 5918
Size: 686 8501
Bme1580I G_kGCm'C
Cuts at: 1790 3336 4284 4562 5869 7248 8404 1790
Size: 1546 948 278 1307 1379 1156 2573
Fragments arranged by size:
2573 1546 1379 1307 1156 948 278
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2115 4224 6918
Size: 416 53 83 399 24 698 2109 2694
Cuts at: 6918 7876 442
Size: 958 1753
Fragments arranged by size:
2694 2109 1753 958 698 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3770 4374 1056
Size: 2714 604 5869
Fragments arranged by size:
5869 2714 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2259 2394 3300 3840 4452 4814 4946 4964
Size: 1020 135 906 540 612 362 132 18
Cuts at: 4964 5398 6466 7847 1239
Size: 434 1068 1381 2579
Fragments arranged by size:
2579 1381 1068 1020 906 612 540 434
362 135 132 18
Bpu10I CC'TnA_GC
Cuts at: 4067 5477 4067
Size: 1410 7777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 7312 8177 8475 4168
Size: 3144 865 298 4880
Fragments arranged by size:
4880 3144 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6445 7850 882
Size: 617 4946 1405 2219
Fragments arranged by size:
4946 2219 1405 617
BsaAI yAC'GTr
Cuts at: 493 1204 6978 493
Size: 711 5774 2702
Fragments arranged by size:
5774 2702 711
BsaBI GATnn'nnATC
Cuts at: 3546 9181 3546
Size: 5635 3552
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4608 6994 7376 8011
Size: 53 83 186 936 3075 2386 382 635
Cuts at: 8011 275
Size: 1451
Fragments arranged by size:
3075 2386 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6324 7620 8018 8292 8439 8850 6324
Size: 1296 398 274 147 411 6661
Fragments arranged by size:
6661 1296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2232 2262 2568 2598 4785
Size: 30 165 30 829 30 306 30 2187
Cuts at: 4785 4815 6092 6122 1178
Size: 30 1277 30 4243
Fragments arranged by size:
4243 2187 1277 829 306 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2406 2808 3012 4748 5963 6484 1186
Size: 1220 402 204 1736 1215 521 3889
Fragments arranged by size:
3889 1736 1220 1215 521 402 204
BseYI C'CCAG_C
Cuts at: 2669 2674 3676 4751 5014 8390 8886 2669
Size: 5 1002 1075 263 3376 496 2970
Fragments arranged by size:
3376 2970 1075 1002 496 263 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3639 1163
Size: 573 1903 6711
Fragments arranged by size:
6711 1903 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6637 7398 8426 1540
Size: 275 186 4636 761 1028 2301
Fragments arranged by size:
4636 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2379 2785 3040 3336 4284 4692 5869 7248 7333
Size: 406 255 296 948 408 1177 1379 85
Cuts at: 7333 8404 2379
Size: 1071 3162
Fragments arranged by size:
3162 1379 1177 1071 948 408 406 296
255 85
BsmI GAATG_Cn'
Cuts at: 3081 3286 3687 5307 6698 6791 3081
Size: 205 401 1620 1391 93 5477
Fragments arranged by size:
5477 1620 1391 401 205 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2379 2785 3040 3336 4284 4562 4692
Size: 399 589 406 255 296 948 278 130
Cuts at: 4692 5118 5497 5869 6276 6506 7248 7333 8404
Size: 426 379 372 407 230 742 85 1071
Cuts at: 8404 1391
Size: 2174
Fragments arranged by size:
2174 1071 948 742 589 426 407 406
399 379 372 296 278 255 230 130
85
BspEI T'CCGG_A
Cuts at: 6324 8850 6324
Size: 2526 6661
BspHI T'CATG_A
Cuts at: 2461 3420 5464 5686 6379 7076 8806 2461
Size: 959 2044 222 693 697 1730 2842
Fragments arranged by size:
2842 2044 1730 959 697 693 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2172 4337 5296 6542 844
Size: 1296 32 2165 959 1246 3489
Fragments arranged by size:
3489 2165 1296 1246 959 32
BsrBI CCG'CTC
Cuts at: 1394 1823 7074 1394
Size: 429 5251 3507
Fragments arranged by size:
5251 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2880 2988 3227 3968 7682 7856 65
Size: 1183 1632 108 239 741 3714 174 1396
Fragments arranged by size:
3714 1632 1396 1183 741 239 174 108
BsrFI r'CCGG_y
Cuts at: 1700 1810 3830 7831 8018 1700
Size: 110 2020 4001 187 2869
Fragments arranged by size:
4001 2869 2020 187 110
BsrGI T'GTAC_A
Cuts at: 96 2551 2857 3271 6418 96
Size: 2455 306 414 3147 2865
Fragments arranged by size:
3147 2865 2455 414 306
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9187
BssSI C'ACGA_G
Cuts at: 2976 7247 8259 2976
Size: 4271 1012 3904
Fragments arranged by size:
4271 3904 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9187
BstBI TT'CG_AA
Cuts at: 7991 7991
Size: 9187
BstEII G'GTnAC_C
Cuts at: 1184 3477 5396 1184
Size: 2293 1919 4975
Fragments arranged by size:
4975 2293 1919
BstXI CCAn_nnnn'nTGG
Cuts at: 2699 6014 2699
Size: 3315 5872
BstYI r'GATC_y
Cuts at: 1972 3070 3696 4225 6477 6517 6884 7269 7286
Size: 1098 626 529 2252 40 367 385 17
Cuts at: 7286 8738 8824 8836 9182 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2252 1977 1452 1098 626 529 385 367
346 86 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 6095 6095
Size: 9187
BtgI C'CryG_G
Cuts at: 513 1065 2535 2647 2656 5031 5518 513
Size: 552 1470 112 9 2375 487 4182
Fragments arranged by size:
4182 2375 1470 552 487 112 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3354 487
Size: 683 56 2128 6320
Fragments arranged by size:
6320 2128 683 56
BtsI GCAGTG_nn'
Cuts at: 2334 2626 3622 3841 4282 4620 4637 5319 5382
Size: 292 996 219 441 338 17 682 63
Cuts at: 5382 5711 5922 6703 7497 7517 2334
Size: 329 211 781 794 20 4004
Fragments arranged by size:
4004 996 794 781 682 441 338 329
292 219 211 63 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4725 4760 529
Size: 35 4161 35 4956
Fragments arranged by size:
4956 4161 35 35
DraI TTT'AAA
Cuts at: 3690 3864 6491 3690
Size: 174 2627 6386
Fragments arranged by size:
6386 2627 174
DraIII CAC_nnn'GTG
Cuts at: 5174 6437 5174
Size: 1263 7924
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8194 809
Size: 507 6878 1802
Fragments arranged by size:
6878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 4681 6634
Size: 54 1425 50 275 18 501 2350 1953
Cuts at: 6634 7523 9152 8
Size: 889 1629 43
Fragments arranged by size:
2350 1953 1629 1425 889 501 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6634 1537
Size: 275 4822 4090
Fragments arranged by size:
4822 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3045 6121 7117 1353
Size: 1692 3076 996 3423
Fragments arranged by size:
3423 3076 1692 996
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7774 8148 8294 387
Size: 1064 138 6185 374 146 1280
Fragments arranged by size:
6185 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2783 2783
Size: 9187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2394 2817 3026 3300 3840 3867
Size: 393 627 135 423 209 274 540 27
Cuts at: 3867 4235 4452 4536 4814 4821 4946 4964 5398
Size: 368 217 84 278 7 125 18 434
Cuts at: 5398 6045 6466 7250 7847 8634 8902 1239
Size: 647 421 784 597 787 268 1524
Fragments arranged by size:
1524 787 784 647 627 597 540 434
423 421 393 368 278 274 268 217
209 135 125 84 27 18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 4266 4839 4266
Size: 573 8614
EcoO109I rG'GnC_Cy
Cuts at: 3437 3498 4176 3437
Size: 61 678 8448
Fragments arranged by size:
8448 678 61
EcoRI G'AATT_C
Cuts at: 5482 6496 6874 5482
Size: 1014 378 7795
Fragments arranged by size:
7795 1014 378
EcoRV GAT'ATC
Cuts at: 3565 3565
Size: 9187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 2209 3092 2066
Size: 143 883 8161
Fragments arranged by size:
8161 883 143
FspI TGC'GCA
Cuts at: 7693 7693
Size: 9187
HaeII r_GCGC'y
Cuts at: 1678 8334 1678
Size: 6656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4378 4410 4844 4870 4876
Size: 32 135 32 2806 32 434 26 6
Cuts at: 4876 4902 4918 4950 5939 5971 6098 6130 6392
Size: 26 16 32 989 32 127 32 262
Cuts at: 6392 6424 7828 7860 7902 7934 1373
Size: 32 1404 32 42 32 2626
Fragments arranged by size:
2806 2626 1404 989 434 262 135 127
42 32 32 32 32 32 32 32
32 32 26 26 16 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 5210 5329 6245 6531 6777
Size: 988 281 1551 1721 119 916 286 246
Cuts at: 6777 669
Size: 3079
Fragments arranged by size:
3079 1721 1551 988 916 286 281 246
119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9187
HpaI GTT'AAC
Cuts at: 6777 6777
Size: 9187
KpnI G_GTAC'C
Cuts at: 3173 4972 6512 3173
Size: 1799 1540 5848
Fragments arranged by size:
5848 1799 1540
MfeI C'AATT_G
Cuts at: 5391 6786 5391
Size: 1395 7792
MluI A'CGCG_T
Cuts at: 9063 9063
Size: 9187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3161 4734 8301 8485 1305
Size: 1856 1573 3567 184 2007
Fragments arranged by size:
3567 2007 1856 1573 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 4683 10
Size: 54 1425 343 501 2350 4514
Fragments arranged by size:
4514 2350 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2185 3153 4469 5874 6012 7145 7504
Size: 648 1019 968 1316 1405 138 1133 359
Cuts at: 7504 7663 518
Size: 159 2042
Fragments arranged by size:
2042 1405 1316 1133 1019 968 648 359
159 138
MspA1I CmG'CkG
Cuts at: 1606 4213 4628 6603 7280 8428 8673 1606
Size: 2607 415 1975 677 1148 245 2120
Fragments arranged by size:
2607 2120 1975 1148 677 415 245
NaeI GCC'GGC
Cuts at: 3832 3832
Size: 9187
NcoI C'CATG_G
Cuts at: 513 1065 2535 2656 5031 5518 513
Size: 552 1470 121 2375 487 4182
Fragments arranged by size:
4182 2375 1470 552 487 121
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9187
NgoMIV G'CCGG_C
Cuts at: 3830 3830
Size: 9187
NheI G'CTAG_C
Cuts at: 1052 3766 4370 1052
Size: 2714 604 5869
Fragments arranged by size:
5869 2714 604
NotI GC'GGCC_GC
Cuts at: 6634 6634
Size: 9187
NsiI A_TGCA'T
Cuts at: 2306 2306
Size: 9187
NspI r_CATG'y
Cuts at: 1132 1229 2184 3193 3895 6545 8090 1132
Size: 97 955 1009 702 2650 1545 2229
Fragments arranged by size:
2650 2229 1545 1009 955 702 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9187
PciI A'CATG_T
Cuts at: 8086 8086
Size: 9187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2648 5204 1108
Size: 1540 2556 5091
Fragments arranged by size:
5091 2556 1540
PfoI T'CCnGG_A
Cuts at: 2236 2386 2236
Size: 150 9037
PmeI GTTT'AAAC
Cuts at: 6491 6491
Size: 9187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6092 6124 7256 7288 8769 8801 6092
Size: 32 1132 32 1481 32 6478
Fragments arranged by size:
6478 1481 1132 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3437 3498 3437
Size: 61 9126
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9187
PsiI TTA'TAA
Cuts at: 6757 6757
Size: 9187
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3264 3296 3264
Size: 32 9155
PstI C_TGCA'G
Cuts at: 830 4042 4627 4644 5326 6539 830
Size: 3212 585 17 682 1213 3478
Fragments arranged by size:
3478 3212 1213 682 585 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9187
PvuII CAG'CTG
Cuts at: 4213 4628 4213
Size: 415 8772
SacI G_AGCT'C
Cuts at: 2785 2785
Size: 9187
SalI G'TCGA_C
Cuts at: 1936 6529 1936
Size: 4593 4594
SanDI GG'GwC_CC
Cuts at: 3498 3498
Size: 9187
SapI GCTCTTCn'nnn_
Cuts at: 3045 3045
Size: 9187
SbfI CC_TGCA'GG
Cuts at: 6539 6539
Size: 9187
ScaI AGT'ACT
Cuts at: 7435 9147 7435
Size: 1712 7475
SexAI A'CCwGG_T
Cuts at: 3244 5937 3244
Size: 2693 6494
SfcI C'TryA_G
Cuts at: 826 1045 3432 4038 4118 4623 4640 4901 5322
Size: 219 2387 606 80 505 17 261 421
Cuts at: 5322 6313 6535 7670 8351 8542 826
Size: 991 222 1135 681 191 1471
Fragments arranged by size:
2387 1471 1135 991 681 606 505 421
261 222 219 191 80 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9187
SmaI CCC'GGG
Cuts at: 1872 4167 6514 1872
Size: 2295 2347 4545
Fragments arranged by size:
4545 2347 2295
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 7291 8192 8454 820
Size: 197 926 2204 3144 901 262 1553
Fragments arranged by size:
3144 2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6978 493
Size: 6485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9187
SphI G_CATG'C
Cuts at: 1132 3193 6545 1132
Size: 2061 3352 3774
Fragments arranged by size:
3774 3352 2061
SspI AAT'ATT
Cuts at: 5 52 5555 7111 5
Size: 47 5503 1556 2081
Fragments arranged by size:
5503 2081 1556 47
StuI AGG'CCT
Cuts at: 1517 2664 1517
Size: 1147 8040
StyI C'CwwG_G
Cuts at: 513 1065 2217 2535 2656 2720 3004 3482 3503
Size: 552 1152 318 121 64 284 478 21
Cuts at: 3503 4990 5031 5518 6143 6626 513
Size: 1487 41 487 625 483 3074
Fragments arranged by size:
3074 1487 1152 625 552 487 483 478
318 284 121 64 41 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3487 5225 5754 6033 7214 7231 7384
Size: 1255 1907 1738 529 279 1181 17 153
Cuts at: 7384 7569 8014 325
Size: 185 445 1498
Fragments arranged by size:
1907 1738 1498 1255 1181 529 445 279
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2551 2857 3271
Size: 274 80 33 51 1443 574 306 414
Cuts at: 3271 3555 3598 5333 6418 7433 9145 96
Size: 284 43 1735 1085 1015 1712 138
Fragments arranged by size:
1735 1712 1443 1085 1015 574 414 306
284 274 138 80 51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2232 2264 5110 5134 5142 5166 9147 9179 2232
Size: 32 2846 24 8 24 3981 32 2240
Fragments arranged by size:
3981 2846 2240 32 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 5895 5939 6167 1316
Size: 4579 44 228 4336
Fragments arranged by size:
4579 4336 228 44
XbaI T'CTAG_A
Cuts at: 5512 5782 6523 5512
Size: 270 741 8176
Fragments arranged by size:
8176 741 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6394 6394
Size: 9187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9187
XmaI C'CCGG_G
Cuts at: 1870 4165 6512 1870
Size: 2295 2347 4545
Fragments arranged by size:
4545 2347 2295
XmnI GAAnn'nnTTC
Cuts at: 2504 3649 7316 2504
Size: 1145 3667 4375
Fragments arranged by size:
4375 3667 1145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6995 276
Size: 53 83 186 936 5461 2468
Fragments arranged by size:
5461 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI
PacI PmlI PspOMI PspXI RsrII SacII SfiI SfoI
SrfI SwaI