Restriction map for
pFN21AA1460
(Circular) (Six-base) MAPSORT of: pFN21AA1460.seq Check: 8802 from: 1 to: 10591
pFN21AA1460 10591 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8401 278
Size: 53 83 186 936 6865 2468
Fragments arranged by size:
6865 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6337 7238 7934 1937
Size: 4400 901 696 4594
Fragments arranged by size:
4594 4400 901 696
Acc65I G'GTAC_C
Cuts at: 3257 7912 3257
Size: 4655 5936
AclI AA'CG_TT
Cuts at: 1563 7050 8718 9091 1563
Size: 5487 1668 373 3063
Fragments arranged by size:
5487 3063 1668 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2595 2739 2805 3257 3312 5004 5223 6164
Size: 963 144 66 452 55 1692 219 941
Cuts at: 6164 7065 8654 10038 10306 1632
Size: 901 1589 1384 268 1917
Fragments arranged by size:
1917 1692 1589 1384 963 941 901 452
268 219 144 66 55
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10394
AflIII A'CryG_T
Cuts at: 1203 2972 4150 5064 7515 9490 10467 1203
Size: 1769 1178 914 2451 1975 977 1327
Fragments arranged by size:
2451 1975 1769 1327 1178 977 914
AgeI A'CCGG_T
Cuts at: 9422 9422
Size: 10591
AhdI GACnn_n'nnGTC
Cuts at: 9320 9320
Size: 10591
AleI CACnn'nnGTG
Cuts at: 1166 5222 7518 7869 1166
Size: 4056 2296 351 3888
Fragments arranged by size:
4056 3888 2296 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4215 1184
Size: 3031 7560
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3151 3183 6200 6232 3151
Size: 32 3017 32 7510
Fragments arranged by size:
7510 3017 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2732 7600 8334 9906 2732
Size: 4868 734 1572 3417
Fragments arranged by size:
4868 3417 1572 734
ApaLI G'TGCA_C
Cuts at: 5919 8648 9804 5919
Size: 2729 1156 6706
Fragments arranged by size:
6706 2729 1156
ApoI r'AATT_y
Cuts at: 1493 2629 3088 3781 5974 6836 7282 7900 8126
Size: 1136 459 693 2193 862 446 618 226
Cuts at: 8126 8278 10387 1493
Size: 152 2109 1697
Fragments arranged by size:
2193 2109 1697 1136 862 693 618 459
446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3177 6933 9145 160
Size: 872 2145 3756 2212 1606
Fragments arranged by size:
3756 2212 2145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10591
AvaI C'yCGr_G
Cuts at: 1870 1943 6036 7628 7815 7916 1870
Size: 73 4093 1592 187 101 4545
Fragments arranged by size:
4545 4093 1592 187 101 73
BamHI G'GATC_C
Cuts at: 2615 7921 8288 2615
Size: 5306 367 4918
Fragments arranged by size:
5306 4918 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3107 3257 3572 7912 9367
Size: 325 217 627 1320 150 315 4340 1455
Cuts at: 9367 618
Size: 1842
Fragments arranged by size:
4340 1842 1455 1320 627 325 315 217
150
BanII G_rGCy'C
Cuts at: 1391 5193 5321 7655 7910 1391
Size: 3802 128 2334 255 4072
Fragments arranged by size:
4072 3802 2334 255 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2643 5802 928
Size: 971 744 3159 5717
Fragments arranged by size:
5717 3159 971 744
BbvCI CC'TCA_GC
Cuts at: 2202 2725 7058 2202
Size: 523 4333 5735
Fragments arranged by size:
5735 4333 523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6356 6390 8782 8816 1903
Size: 34 4419 34 2392 34 3678
Fragments arranged by size:
4419 3678 2392 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2409 4201 8483 9693 2409
Size: 1792 4282 1210 3307
Fragments arranged by size:
4282 3307 1792 1210
BclI T'GATC_A
Cuts at: 1548 4099 7425 1548
Size: 2551 3326 4714
Fragments arranged by size:
4714 3326 2551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3618 5421 7650
Size: 107 122 71 631 605 1946 1803 2229
Cuts at: 7650 9202 136
Size: 1552 1525
Fragments arranged by size:
2229 1946 1803 1552 1525 631 605 122
107 71
BglII A'GATC_T
Cuts at: 6240 10586 6240
Size: 4346 6245
BlpI GC'TnA_GC
Cuts at: 8008 8008
Size: 10591
Bme1580I G_kGCm'C
Cuts at: 1790 3044 3686 5923 8652 9808 1790
Size: 1254 642 2237 2729 1156 2573
Fragments arranged by size:
2729 2573 2237 1254 1156 642
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10591
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2181 2943 4074
Size: 416 53 83 399 24 764 762 1131
Cuts at: 4074 4582 5994 6241 7535 8322 9280 442
Size: 508 1412 247 1294 787 958 1753
Fragments arranged by size:
1753 1412 1294 1131 958 787 764 762
508 416 399 247 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3099 1056
Size: 2043 8548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5503 7345 5503
Size: 1842 8749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2767 3615 3807 4818 5196 5305 5715 6039
Size: 1528 848 192 1011 378 109 410 324
Cuts at: 6039 6649 6747 7663 9251 1239
Size: 610 98 916 1588 2579
Fragments arranged by size:
2579 1588 1528 1011 916 848 610 410
378 324 192 109 98
Bpu10I CC'TnA_GC
Cuts at: 2202 2725 3524 4627 6492 7058 7330 2202
Size: 523 799 1103 1865 566 272 5463
Fragments arranged by size:
5463 1865 1103 799 566 523 272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4070 5460 6081 8716 9581 9879 4070
Size: 1390 621 2635 865 298 4782
Fragments arranged by size:
4782 2635 1390 865 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2986 4767 7119 7731 9254 882
Size: 617 1487 1781 2352 612 1523 2219
Fragments arranged by size:
2352 2219 1781 1523 1487 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 4151 5313 6791 7242 8382 493
Size: 711 2947 1162 1478 451 1140 2702
Fragments arranged by size:
2947 2702 1478 1162 1140 711 451
BsaBI GATnn'nnATC
Cuts at: 3928 5757 7695 10585 3928
Size: 1829 1938 2890 3934
Fragments arranged by size:
3934 2890 1938 1829
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4045 7290 8398 8780
Size: 53 83 186 936 2512 3245 1108 382
Cuts at: 8780 9415 275
Size: 635 1451
Fragments arranged by size:
3245 2512 1451 1108 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2743 3404 4658 9024 9422 9696 9843 10254 2743
Size: 661 1254 4366 398 274 147 411 3080
Fragments arranged by size:
4366 3080 1254 661 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3151 3181 3705 3735 3996
Size: 30 165 30 1748 30 524 30 261
Cuts at: 3996 4026 7099 7129 7594 7624 7661 7691 1178
Size: 30 3073 30 465 30 37 30 4078
Fragments arranged by size:
4078 3073 1748 524 465 261 165 37
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4490 6096 7580 1186
Size: 3304 1606 1484 4197
Fragments arranged by size:
4197 3304 1606 1484
BseYI C'CCAG_C
Cuts at: 2634 3842 4915 4936 4962 5181 5270 5274 5424
Size: 1208 1073 21 26 219 89 4 150
Cuts at: 5424 5956 6219 7314 7648 7712 9794 10290 2634
Size: 532 263 1095 334 64 2082 496 2935
Fragments arranged by size:
2935 2082 1208 1095 1073 532 496 334
263 219 150 89 64 26 21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2591 3208 3434 3756 3801 6371 7496
Size: 573 855 617 226 322 45 2570 1125
Cuts at: 7496 1163
Size: 4258
Fragments arranged by size:
4258 2570 1125 855 617 573 322 226
45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5769 6317 8041 8802 9830 1540
Size: 275 186 3768 548 1724 761 1028 2301
Fragments arranged by size:
3768 2301 1724 1028 761 548 275 186
BsiHKAI G_wGCw'C
Cuts at: 3464 5923 6097 8652 8737 9808 3464
Size: 2459 174 2555 85 1071 4247
Fragments arranged by size:
4247 2555 2459 1071 174 85
BsmI GAATG_Cn'
Cuts at: 2689 3037 3649 3877 8102 8195 2689
Size: 348 612 228 4225 93 5085
Fragments arranged by size:
5085 4225 612 348 228 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3044 3464 3686 5193 5321 5923 6097
Size: 399 1254 420 222 1507 128 602 174
Cuts at: 6097 7655 7910 8652 8737 9808 1391
Size: 1558 255 742 85 1071 2174
Fragments arranged by size:
2174 1558 1507 1254 1071 742 602 420
399 255 222 174 128 85
BspEI T'CCGG_A
Cuts at: 2743 10254 2743
Size: 7511 3080
BspHI T'CATG_A
Cuts at: 7147 8480 10210 7147
Size: 1333 1730 7528
Fragments arranged by size:
7528 1730 1333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4375 7946 844
Size: 3531 3571 3489
Fragments arranged by size:
3571 3531 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 8478 1394
Size: 429 6655 3507
Fragments arranged by size:
6655 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3535 3907 5286 5399 6137 9086 9260
Size: 1183 2287 372 1379 113 738 2949 174
Cuts at: 9260 65
Size: 1396
Fragments arranged by size:
2949 2287 1396 1379 1183 738 372 174
113
BsrFI r'CCGG_y
Cuts at: 1700 1810 6312 7644 9235 9422 1700
Size: 110 4502 1332 1591 187 2869
Fragments arranged by size:
4502 2869 1591 1332 187 110
BsrGI T'GTAC_A
Cuts at: 96 5668 96
Size: 5572 5019
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10591
BssSI C'ACGA_G
Cuts at: 2403 8651 9663 2403
Size: 6248 1012 3331
Fragments arranged by size:
6248 3331 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6364 7404 794
Size: 5570 1040 3981
Fragments arranged by size:
5570 3981 1040
BstBI TT'CG_AA
Cuts at: 9395 9395
Size: 10591
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10591
BstXI CCAn_nnnn'nTGG
Cuts at: 3629 5224 5939 7871 3629
Size: 1595 715 1932 6349
Fragments arranged by size:
6349 1932 1595 715
BstYI r'GATC_y
Cuts at: 1972 2492 2615 4144 4865 5032 6240 7921 8288
Size: 520 123 1529 721 167 1208 1681 367
Cuts at: 8288 8673 8690 10142 10228 10240 10586 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1681 1529 1452 1208 721 520 385
367 346 167 123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6123 6959 6123
Size: 836 9755
BtgI C'CryG_G
Cuts at: 513 1065 2920 2939 2953 3103 3253 5814 6854
Size: 552 1855 19 14 150 150 2561 1040
Cuts at: 6854 7887 513
Size: 1033 3217
Fragments arranged by size:
3217 2561 1855 1040 1033 552 150 150
19 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4035 487
Size: 683 56 2809 7043
Fragments arranged by size:
7043 2809 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 2743 3991 5176 6823 6916 7185 8107 8901
Size: 207 1248 1185 1647 93 269 922 794
Cuts at: 8901 8921 2536
Size: 20 4206
Fragments arranged by size:
4206 1647 1248 1185 922 794 269 207
93 20
ClaI AT'CG_AT
Cuts at: 1554 7380 1554
Size: 5826 4765
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2370 2405 3697 3732 529
Size: 35 1806 35 1292 35 7388
Fragments arranged by size:
7388 1806 1292 35 35 35
DraI TTT'AAA
Cuts at: 5496 7895 5496
Size: 2399 8192
DraIII CAC_nnn'GTG
Cuts at: 7404 7404
Size: 10591
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6896 7218 9598 809
Size: 507 5580 322 2380 1802
Fragments arranged by size:
5580 2380 1802 507 322
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3113 3619 5501
Size: 54 1425 50 275 18 1283 506 1882
Cuts at: 5501 6314 6785 6827 8038 8927 10556 8
Size: 813 471 42 1211 889 1629 43
Fragments arranged by size:
1882 1629 1425 1283 1211 889 813 506
471 275 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 6314 8038 1537
Size: 275 4502 1724 4090
Fragments arranged by size:
4502 4090 1724 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3793 3936 5424 5430 6699 7479 7557 8521
Size: 2440 143 1488 6 1269 780 78 964
Cuts at: 8521 1353
Size: 3423
Fragments arranged by size:
3423 2440 1488 1269 964 780 143 78
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9178 9552 9698 387
Size: 1064 138 7589 374 146 1280
Fragments arranged by size:
7589 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2595 2739 2767 2805 3257 3312 3615
Size: 393 963 144 28 38 452 55 303
Cuts at: 3615 3807 4818 5004 5196 5223 5305 5715 6039
Size: 192 1011 186 192 27 82 410 324
Cuts at: 6039 6164 6649 6747 7065 7663 8654 9251 10038
Size: 125 485 98 318 598 991 597 787
Cuts at: 10038 10306 1239
Size: 268 1524
Fragments arranged by size:
1524 1011 991 963 787 598 597 485
452 410 393 324 318 303 268 192
192 186 144 125 98 82 55 38
28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 4905 4905
Size: 10591
EcoO109I rG'GnC_Cy
Cuts at: 2642 3149 3447 3611 4922 5777 5935 7162 7764
Size: 507 298 164 1311 855 158 1227 602
Cuts at: 7764 7793 2642
Size: 29 5440
Fragments arranged by size:
5440 1311 1227 855 602 507 298 164
158 29
EcoRI G'AATT_C
Cuts at: 3088 5974 7900 8278 3088
Size: 2886 1926 378 5401
Fragments arranged by size:
5401 2886 1926 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2550 2867 6318 7249 2550
Size: 317 3451 931 5892
Fragments arranged by size:
5892 3451 931 317
FspI TGC'GCA
Cuts at: 7397 9097 7397
Size: 1700 8891
HaeII r_GCGC'y
Cuts at: 1678 9738 1678
Size: 8060 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2765 2797 3472 3504 4322
Size: 32 135 32 1193 32 675 32 818
Cuts at: 4322 4354 4958 4990 6584 6616 6647 6679 7099
Size: 32 604 32 1594 32 31 32 420
Cuts at: 7099 7131 7594 7626 9232 9264 9306 9338 1373
Size: 32 463 32 1606 32 42 32 2626
Fragments arranged by size:
2626 1606 1594 1193 818 675 604 463
420 135 42 32 32 32 32 32
32 32 32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 3329 3700 6338 6380 6952 7861
Size: 988 281 1391 371 2638 42 572 909
Cuts at: 7861 7935 8181 669
Size: 74 246 3079
Fragments arranged by size:
3079 2638 1391 988 909 572 371 281
246 74 42
HindIII A'AGCT_T
Cuts at: 748 5900 748
Size: 5152 5439
HpaI GTT'AAC
Cuts at: 3700 8181 3700
Size: 4481 6110
KpnI G_GTAC'C
Cuts at: 3261 7916 3261
Size: 4655 5936
MfeI C'AATT_G
Cuts at: 8190 8190
Size: 10591
MluI A'CGCG_T
Cuts at: 10467 10467
Size: 10591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2685 4185 4643 4843 5995 7065 9705 9889
Size: 1380 1500 458 200 1152 1070 2640 184
Cuts at: 9889 1305
Size: 2007
Fragments arranged by size:
2640 2007 1500 1380 1152 1070 458 200
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3115 3621 5503 6787 6829
Size: 54 1425 343 1283 506 1882 1284 42
Cuts at: 6829 10
Size: 3772
Fragments arranged by size:
3772 1882 1425 1284 1283 506 343 54
42
MslI CAynn'nnrTG
Cuts at: 518 1166 2813 3350 3882 4602 4729 5222 5311
Size: 648 1647 537 532 720 127 493 89
Cuts at: 5311 6638 7455 7518 7704 7869 8549 8908 9067
Size: 1327 817 63 186 165 680 359 159
Cuts at: 9067 518
Size: 2042
Fragments arranged by size:
2042 1647 1327 817 720 680 648 537
532 493 359 186 165 159 127 89
63
MspA1I CmG'CkG
Cuts at: 1606 2638 5128 5274 7314 7423 8007 8684 9832
Size: 1032 2490 146 2040 109 584 677 1148
Cuts at: 9832 10077 1606
Size: 245 2120
Fragments arranged by size:
2490 2120 2040 1148 1032 677 584 245
146 109
NaeI GCC'GGC
Cuts at: 7646 7646
Size: 10591
NcoI C'CATG_G
Cuts at: 513 1065 2920 2939 2953 3103 3253 5814 6854
Size: 552 1855 19 14 150 150 2561 1040
Cuts at: 6854 7887 513
Size: 1033 3217
Fragments arranged by size:
3217 2561 1855 1040 1033 552 150 150
19 14
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10591
NgoMIV G'CCGG_C
Cuts at: 7644 7644
Size: 10591
NheI G'CTAG_C
Cuts at: 1052 3095 1052
Size: 2043 8548
NotI GC'GGCC_GC
Cuts at: 8038 8038
Size: 10591
NspI r_CATG'y
Cuts at: 1132 1229 2976 4302 5068 5875 6046 7411 7519
Size: 97 1747 1326 766 807 171 1365 108
Cuts at: 7519 7949 9494 1132
Size: 430 1545 2229
Fragments arranged by size:
2229 1747 1545 1365 1326 807 766 430
171 108 97
PasI CC'CwG_GG
Cuts at: 1348 4762 5193 1348
Size: 3414 431 6746
Fragments arranged by size:
6746 3414 431
PciI A'CATG_T
Cuts at: 2972 5064 7515 9490 2972
Size: 2092 2451 1975 4073
Fragments arranged by size:
4073 2451 2092 1975
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4962 5405 5424 6182 1108
Size: 3854 443 19 758 5517
Fragments arranged by size:
5517 3854 758 443 19
PfoI T'CCnGG_A
Cuts at: 7207 7207
Size: 10591
PmeI GTTT'AAAC
Cuts at: 7895 7895
Size: 10591
PmlI CAC'GTG
Cuts at: 4151 5313 6791 4151
Size: 1162 1478 7951
Fragments arranged by size:
7951 1478 1162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3151 3183 3229 3261 8660 8692 10173 10205 3151
Size: 32 46 32 5399 32 1481 32 3537
Fragments arranged by size:
5399 3537 1481 46 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2642 4922 7162 7764 7793 2642
Size: 2280 2240 602 29 5440
Fragments arranged by size:
5440 2280 2240 602 29
PshAI GACnn'nnGTC
Cuts at: 1613 2647 5265 1613
Size: 1034 2618 6939
Fragments arranged by size:
6939 2618 1034
PsiI TTA'TAA
Cuts at: 8161 8161
Size: 10591
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4669 4701 4669
Size: 32 10559
PstI C_TGCA'G
Cuts at: 830 7943 830
Size: 7113 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10591
PvuII CAG'CTG
Cuts at: 5128 5274 7314 5128
Size: 146 2040 8405
Fragments arranged by size:
8405 2040 146
RsrII CG'GwC_CG
Cuts at: 7686 7686
Size: 10591
SalI G'TCGA_C
Cuts at: 1936 6336 7933 1936
Size: 4400 1597 4594
Fragments arranged by size:
4594 4400 1597
SanDI GG'GwC_CC
Cuts at: 7764 7793 7764
Size: 29 10562
SbfI CC_TGCA'GG
Cuts at: 7943 7943
Size: 10591
ScaI AGT'ACT
Cuts at: 2608 2932 3193 6745 8839 10551 2608
Size: 324 261 3552 2094 1712 2648
Fragments arranged by size:
3552 2648 2094 1712 324 261
SfcI C'TryA_G
Cuts at: 826 1045 2268 3153 3195 4434 4887 6540 7471
Size: 219 1223 885 42 1239 453 1653 931
Cuts at: 7471 7939 9074 9755 9946 826
Size: 468 1135 681 191 1471
Fragments arranged by size:
1653 1471 1239 1223 1135 931 885 681
468 453 219 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 3618 3618
Size: 10591
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10591
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10591
SmaI CCC'GGG
Cuts at: 1872 6038 7918 1872
Size: 4166 1880 4545
Fragments arranged by size:
4545 4166 1880
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4049 5475 6096 8695 9596 9858
Size: 197 926 2106 1426 621 2599 901 262
Cuts at: 9858 820
Size: 1553
Fragments arranged by size:
2599 2106 1553 1426 926 901 621 262
197
SnaBI TAC'GTA
Cuts at: 493 8382 493
Size: 7889 2702
SpeI A'CTAG_T
Cuts at: 152 4682 7013 152
Size: 4530 2331 3730
Fragments arranged by size:
4530 3730 2331
SphI G_CATG'C
Cuts at: 1132 6046 7411 7949 1132
Size: 4914 1365 538 3774
Fragments arranged by size:
4914 3774 1365 538
SspI AAT'ATT
Cuts at: 5 52 3181 3340 5746 8515 5
Size: 47 3129 159 2406 2769 2081
Fragments arranged by size:
3129 2769 2406 2081 159 47
StuI AGG'CCT
Cuts at: 1517 2387 4259 1517
Size: 870 1872 7849
Fragments arranged by size:
7849 1872 870
StyI C'CwwG_G
Cuts at: 513 1065 2488 2920 2939 2953 3103 3253 4543
Size: 552 1423 432 19 14 150 150 1290
Cuts at: 4543 5814 5938 6854 7887 8030 513
Size: 1271 124 916 1033 143 3074
Fragments arranged by size:
3074 1423 1290 1271 1033 916 552 432
150 150 143 124 19 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7703 7859 8618 8635 8788 8973 9418
Size: 1255 6123 156 759 17 153 185 445
Cuts at: 9418 325
Size: 1498
Fragments arranged by size:
6123 1498 1255 759 445 185 156 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2606 2930 3191
Size: 274 80 33 51 1443 629 324 261
Cuts at: 3191 3752 4028 5668 6743 7064 7522 8837 10549
Size: 561 276 1640 1075 321 458 1315 1712
Cuts at: 10549 96
Size: 138
Fragments arranged by size:
1712 1640 1443 1315 1075 629 561 458
324 321 276 274 261 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2818 2850 3705 3737 3996 4028 7661 7693 10551
Size: 32 855 32 259 32 3633 32 2858
Cuts at: 10551 10583 2818
Size: 32 2826
Fragments arranged by size:
3633 2858 2826 855 259 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6470 7111 1316
Size: 5154 641 4796
Fragments arranged by size:
5154 4796 641
XbaI T'CTAG_A
Cuts at: 7927 7927
Size: 10591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 5953 7311 2011
Size: 3942 1358 5291
Fragments arranged by size:
5291 3942 1358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10591
XmaI C'CCGG_G
Cuts at: 1870 6036 7916 1870
Size: 4166 1880 4545
Fragments arranged by size:
4545 4166 1880
XmnI GAAnn'nnTTC
Cuts at: 6458 8720 6458
Size: 2262 8329
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8399 276
Size: 53 83 186 936 6865 2468
Fragments arranged by size:
6865 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI SacI SacII
SapI SexAI SfoI SrfI SwaI