Restriction map for
pFN21AA1454
(Circular) (Six-base) MAPSORT of: pFN21AA1454.seq Check: 3710 from: 1 to: 8902
pFN21AA1454 8902 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5000 5000
Size: 8902
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6712 278
Size: 53 83 186 936 5176 2468
Fragments arranged by size:
5176 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2228 6245 1937
Size: 291 4017 4594
Fragments arranged by size:
4594 4017 291
Acc65I G'GTAC_C
Cuts at: 6223 6223
Size: 8902
AclI AA'CG_TT
Cuts at: 1563 7029 7402 1563
Size: 5466 373 3063
Fragments arranged by size:
5466 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 2694 2808 3219 3357 3390 3692 4392
Size: 812 250 114 411 138 33 302 700
Cuts at: 4392 4627 4700 5510 6965 8349 8617 1632
Size: 235 73 810 1455 1384 268 1917
Fragments arranged by size:
1917 1455 1384 812 810 700 411 302
268 250 235 138 114 73 33
AfeI AGC'GCT
Cuts at: 2021 2021
Size: 8902
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8705
AflIII A'CryG_T
Cuts at: 1203 7801 8778 1203
Size: 6598 977 1327
Fragments arranged by size:
6598 1327 977
AgeI A'CCGG_T
Cuts at: 7733 7733
Size: 8902
AhdI GACnn_n'nnGTC
Cuts at: 6066 7631 6066
Size: 1565 7337
AleI CACnn'nnGTG
Cuts at: 1166 3006 4925 1166
Size: 1840 1919 5143
Fragments arranged by size:
5143 1919 1840
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8902
AlwNI CAG_nnn'CTG
Cuts at: 6645 8217 6645
Size: 1572 7330
ApaLI G'TGCA_C
Cuts at: 6959 8115 6959
Size: 1156 7746
ApoI r'AATT_y
Cuts at: 1493 2605 3067 3191 3247 3573 4236 4681 4944
Size: 1112 462 124 56 326 663 445 263
Cuts at: 4944 6211 6437 6589 8698 1493
Size: 1267 226 152 2109 1697
Fragments arranged by size:
2109 1697 1267 1112 663 462 445 326
263 226 152 124 56
AseI AT'TA_AT
Cuts at: 160 1032 7456 160
Size: 872 6424 1606
Fragments arranged by size:
6424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8902
AvaI C'yCGr_G
Cuts at: 1870 1943 4152 6227 1870
Size: 73 2209 2075 4545
Fragments arranged by size:
4545 2209 2075 73
AvrII C'CTAG_G
Cuts at: 4807 4807
Size: 8902
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5057 5090 5057
Size: 33 8869
BamHI G'GATC_C
Cuts at: 5500 6232 6599 5500
Size: 732 367 7803
Fragments arranged by size:
7803 732 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 3510 5582 6223 7678
Size: 325 217 627 487 1236 2072 641 1455
Cuts at: 7678 618
Size: 1842
Fragments arranged by size:
2072 1842 1455 1236 641 627 487 325
217
BanII G_rGCy'C
Cuts at: 1391 2302 4039 6221 1391
Size: 911 1737 2182 4072
Fragments arranged by size:
4072 2182 1737 911
BbeI G_GCGC'C
Cuts at: 3514 3514
Size: 8902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2483 3991 928
Size: 971 584 1508 5839
Fragments arranged by size:
5839 1508 971 584
BbvCI CC'TCA_GC
Cuts at: 6150 6150
Size: 8902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3464 3498 7093 7127 1903
Size: 34 1527 34 3595 34 3678
Fragments arranged by size:
3678 3595 1527 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6794 8004 6794
Size: 1210 7692
BclI T'GATC_A
Cuts at: 1548 5940 1548
Size: 4392 4510
BfrBI ATG'CAT
Cuts at: 3583 3583
Size: 8902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7513 136
Size: 107 122 71 631 605 5841 1525
Fragments arranged by size:
5841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4732 8897 4732
Size: 4165 4737
BlpI GC'TnA_GC
Cuts at: 4308 4313 6319 4308
Size: 5 2006 6891
Fragments arranged by size:
6891 2006 5
Bme1580I G_kGCm'C
Cuts at: 1790 6963 8119 1790
Size: 5173 1156 2573
Fragments arranged by size:
5173 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 2722 3028
Size: 416 53 83 399 24 900 405 306
Cuts at: 3028 5369 6633 7591 442
Size: 2341 1264 958 1753
Fragments arranged by size:
2341 1753 1264 958 900 416 405 399
306 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3287 1056
Size: 2231 6671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 3546 4548 5565 6026 6048 7562 1239
Size: 1017 1290 1002 1017 461 22 1514 2579
Fragments arranged by size:
2579 1514 1290 1017 1017 1002 461 22
Bpu10I CC'TnA_GC
Cuts at: 3671 3864 4650 6150 3671
Size: 193 786 1500 6423
Fragments arranged by size:
6423 1500 786 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4229 6149 7027 7892 8190 4229
Size: 1920 878 865 298 4941
Fragments arranged by size:
4941 1920 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2792 7565 882
Size: 617 1293 4773 2219
Fragments arranged by size:
4773 2219 1293 617
BsaAI yAC'GTr
Cuts at: 493 1204 4007 5317 6693 493
Size: 711 2803 1310 1376 2702
Fragments arranged by size:
2803 2702 1376 1310 711
BsaBI GATnn'nnATC
Cuts at: 5385 8896 5385
Size: 3511 5391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2539 3511 6709 7091
Size: 53 83 186 936 1006 972 3198 382
Cuts at: 7091 7726 275
Size: 635 1451
Fragments arranged by size:
3198 1451 1006 972 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3738 7335 7733 8007 8154 8565 3738
Size: 3597 398 274 147 411 4075
Fragments arranged by size:
4075 3597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8677
Fragments arranged by size:
8677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2628 1186
Size: 889 553 7460
Fragments arranged by size:
7460 889 553
BseYI C'CCAG_C
Cuts at: 4915 5597 5764 8105 8601 4915
Size: 682 167 2341 496 5216
Fragments arranged by size:
5216 2341 682 496 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2771 3842 4040 5885 1163
Size: 573 1035 1071 198 1845 4180
Fragments arranged by size:
4180 1845 1071 1035 573 198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5875 6352 7113 8141 1540
Size: 275 186 3874 477 761 1028 2301
Fragments arranged by size:
3874 2301 1028 761 477 275 186
BsiHKAI G_wGCw'C
Cuts at: 2302 4039 6963 7048 8119 2302
Size: 1737 2924 85 1071 3085
Fragments arranged by size:
3085 2924 1737 1071 85
BsmI GAATG_Cn'
Cuts at: 5738 6413 6506 5738
Size: 675 93 8134
Fragments arranged by size:
8134 675 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2302 4039 6221 6963 7048 8119 1391
Size: 399 512 1737 2182 742 85 1071 2174
Fragments arranged by size:
2182 2174 1737 1071 742 512 399 85
BspEI T'CCGG_A
Cuts at: 3738 8565 3738
Size: 4827 4075
BspHI T'CATG_A
Cuts at: 2032 3916 5803 6791 8521 2032
Size: 1884 1887 988 1730 2413
Fragments arranged by size:
2413 1887 1884 1730 988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3559 5000 6257 844
Size: 2715 1441 1257 3489
Fragments arranged by size:
3489 2715 1441 1257
BsrBI CCG'CTC
Cuts at: 1394 1823 4051 4594 6789 1394
Size: 429 2228 543 2195 3507
Fragments arranged by size:
3507 2228 2195 543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3318 4399 4972 5442 7397 7571 65
Size: 1183 2070 1081 573 470 1955 174 1396
Fragments arranged by size:
2070 1955 1396 1183 1081 573 470 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7546 7733 1700
Size: 110 5736 187 2869
Fragments arranged by size:
5736 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5441 96
Size: 5345 3557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8902
BssSI C'ACGA_G
Cuts at: 3498 6962 7974 3498
Size: 3464 1012 4426
Fragments arranged by size:
4426 3464 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8902
BstBI TT'CG_AA
Cuts at: 3097 4264 7706 3097
Size: 1167 3442 4293
Fragments arranged by size:
4293 3442 1167
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8902
BstXI CCAn_nnnn'nTGG
Cuts at: 5644 5644
Size: 8902
BstYI r'GATC_y
Cuts at: 1972 4732 5500 6232 6599 6984 7001 8453 8539
Size: 2760 768 732 367 385 17 1452 86
Cuts at: 8539 8551 8897 1972
Size: 12 346 1977
Fragments arranged by size:
2760 1977 1452 768 732 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3252 3462 5578 5933 513
Size: 552 939 1248 210 2116 355 3482
Fragments arranged by size:
3482 2116 1248 939 552 355 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8163
Fragments arranged by size:
8163 683 56
BtsI GCAGTG_nn'
Cuts at: 2211 2847 3439 4324 4385 5023 5601 5770 6418
Size: 636 592 885 61 638 578 169 648
Cuts at: 6418 7212 7232 2211
Size: 794 20 3881
Fragments arranged by size:
3881 885 794 648 638 636 592 578
169 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8867
DraI TTT'AAA
Cuts at: 6206 6206
Size: 8902
DraIII CAC_nnn'GTG
Cuts at: 5276 5276
Size: 8902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7909 809
Size: 507 6593 1802
Fragments arranged by size:
6593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5397 5646 5751
Size: 54 1425 50 275 18 3567 249 105
Cuts at: 5751 5872 6033 6349 7238 8867 8
Size: 121 161 316 889 1629 43
Fragments arranged by size:
3567 1629 1425 889 316 275 249 161
121 105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5872 6349 1537
Size: 275 4060 477 4090
Fragments arranged by size:
4090 4060 477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3273 3684 3966 4076 4215 4411 4584 5529
Size: 1920 411 282 110 139 196 173 945
Cuts at: 5529 5579 5691 6832 1353
Size: 50 112 1141 3423
Fragments arranged by size:
3423 1920 1141 945 411 282 196 173
139 112 110 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7489 7863 8009 387
Size: 1064 138 5900 374 146 1280
Fragments arranged by size:
5900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2300 4037 2300
Size: 1737 7165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2444 2694 2808 3219 3357 3390
Size: 393 624 188 250 114 411 138 33
Cuts at: 3390 3546 3692 4392 4548 4627 4700 5510 5565
Size: 156 146 700 156 79 73 810 55
Cuts at: 5565 6026 6048 6965 7562 8349 8617 1239
Size: 461 22 917 597 787 268 1524
Fragments arranged by size:
1524 917 810 787 700 624 597 461
411 393 268 250 188 156 156 146
138 114 79 73 55 33 22
EcoNI CCTnn'n_nnAGG
Cuts at: 5804 5804
Size: 8902
EcoO109I rG'GnC_Cy
Cuts at: 5302 5302
Size: 8902
EcoRI G'AATT_C
Cuts at: 3067 3573 4944 6211 6589 3067
Size: 506 1371 1267 378 5380
Fragments arranged by size:
5380 1371 1267 506 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4730 5062 6143 4730
Size: 332 1081 7489
Fragments arranged by size:
7489 1081 332
FspI TGC'GCA
Cuts at: 7408 7408
Size: 8902
HaeII r_GCGC'y
Cuts at: 1678 2023 3514 8049 1678
Size: 345 1491 4535 2531
Fragments arranged by size:
4535 2531 1491 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2502 2539 2571 3911
Size: 32 135 32 898 32 37 32 1340
Cuts at: 3911 3943 4751 4783 5185 5217 5506 5538 5714
Size: 32 808 32 402 32 289 32 176
Cuts at: 5714 5746 6052 6084 7543 7575 7617 7649 1373
Size: 32 306 32 1459 32 42 32 2626
Fragments arranged by size:
2626 1459 1340 898 808 402 306 289
176 135 42 37 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 5076 5839 6246 6492 669
Size: 988 281 1413 1725 763 407 246 3079
Fragments arranged by size:
3079 1725 1413 988 763 407 281 246
HindIII A'AGCT_T
Cuts at: 748 2599 3704 4572 4712 748
Size: 1851 1105 868 140 4938
Fragments arranged by size:
4938 1851 1105 868 140
HpaI GTT'AAC
Cuts at: 3351 6492 3351
Size: 3141 5761
KasI G'GCGC_C
Cuts at: 3510 3510
Size: 8902
KpnI G_GTAC'C
Cuts at: 6227 6227
Size: 8902
MfeI C'AATT_G
Cuts at: 3618 3834 6501 3618
Size: 216 2667 6019
Fragments arranged by size:
6019 2667 216
MluI A'CGCG_T
Cuts at: 8778 8778
Size: 8902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2932 8016 8200 1305
Size: 1627 5084 184 2007
Fragments arranged by size:
5084 2007 1627 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5399 5648 5753 6035 10
Size: 54 1425 343 3567 249 105 282 2877
Fragments arranged by size:
3567 2877 1425 343 282 249 105 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2509 3006 3582 4925 5642 6860 7219
Size: 648 1343 497 576 1343 717 1218 359
Cuts at: 7219 7378 518
Size: 159 2042
Fragments arranged by size:
2042 1343 1343 1218 717 648 576 497
359 159
MspA1I CmG'CkG
Cuts at: 1606 4199 5519 6318 6995 8143 8388 1606
Size: 2593 1320 799 677 1148 245 2120
Fragments arranged by size:
2593 2120 1320 1148 799 677 245
NarI GG'CG_CC
Cuts at: 3511 3511
Size: 8902
NcoI C'CATG_G
Cuts at: 513 1065 2004 3252 3462 5578 513
Size: 552 939 1248 210 2116 3837
Fragments arranged by size:
3837 2116 1248 939 552 210
NdeI CA'TA_TG
Cuts at: 387 5556 387
Size: 5169 3733
NheI G'CTAG_C
Cuts at: 1052 3283 1052
Size: 2231 6671
NotI GC'GGCC_GC
Cuts at: 6349 6349
Size: 8902
NsiI A_TGCA'T
Cuts at: 3585 3585
Size: 8902
NspI r_CATG'y
Cuts at: 1132 1229 3583 4331 5417 5435 6260 7805 1132
Size: 97 2354 748 1086 18 825 1545 2229
Fragments arranged by size:
2354 2229 1545 1086 825 748 97 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8902
PciI A'CATG_T
Cuts at: 7801 7801
Size: 8902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3468 3569 1108
Size: 2360 101 6441
Fragments arranged by size:
6441 2360 101
PfoI T'CCnGG_A
Cuts at: 2533 2533
Size: 8902
PmeI GTTT'AAAC
Cuts at: 6206 6206
Size: 8902
PmlI CAC'GTG
Cuts at: 4007 4007
Size: 8902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6971 7003 8484 8516 6971
Size: 32 1481 32 7357
Fragments arranged by size:
7357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5302 5302
Size: 8902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8902
PsiI TTA'TAA
Cuts at: 3887 4704 6472 3887
Size: 817 1768 6317
Fragments arranged by size:
6317 1768 817
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2953 2985 2953
Size: 32 8870
PstI C_TGCA'G
Cuts at: 830 2206 5018 5412 5777 5895 6254 830
Size: 1376 2812 394 365 118 359 3478
Fragments arranged by size:
3478 2812 1376 394 365 359 118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8902
PvuII CAG'CTG
Cuts at: 4199 5519 4199
Size: 1320 7582
SacI G_AGCT'C
Cuts at: 2302 4039 2302
Size: 1737 7165
SalI G'TCGA_C
Cuts at: 1936 6244 1936
Size: 4308 4594
SapI GCTCTTCn'nnn_
Cuts at: 3273 3966 4584 5529 5691 3273
Size: 693 618 945 162 6484
Fragments arranged by size:
6484 945 693 618 162
SbfI CC_TGCA'GG
Cuts at: 6254 6254
Size: 8902
ScaI AGT'ACT
Cuts at: 2200 5949 7150 8862 2200
Size: 3749 1201 1712 2240
Fragments arranged by size:
3749 2240 1712 1201
SexAI A'CCwGG_T
Cuts at: 5853 5853
Size: 8902
SfcI C'TryA_G
Cuts at: 826 1045 2202 2973 3965 5014 5408 5773 5891
Size: 219 1157 771 992 1049 394 365 118
Cuts at: 5891 6250 7385 8066 8257 826
Size: 359 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 1049 992 771 681 394
365 359 219 191 118
SfoI GGC'GCC
Cuts at: 3512 3512
Size: 8902
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8902
SmaI CCC'GGG
Cuts at: 1872 4154 6229 1872
Size: 2282 2075 4545
Fragments arranged by size:
4545 2282 2075
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4244 6128 7006 7907 8169 820
Size: 197 926 2301 1884 878 901 262 1553
Fragments arranged by size:
2301 1884 1553 926 901 878 262 197
SnaBI TAC'GTA
Cuts at: 493 6693 493
Size: 6200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8902
SphI G_CATG'C
Cuts at: 1132 5417 6260 1132
Size: 4285 843 3774
Fragments arranged by size:
4285 3774 843
SspI AAT'ATT
Cuts at: 5 52 6826 5
Size: 47 6774 2081
Fragments arranged by size:
6774 2081 47
StuI AGG'CCT
Cuts at: 1517 2759 2818 6158 1517
Size: 1242 59 3340 4261
Fragments arranged by size:
4261 3340 1242 59
StyI C'CwwG_G
Cuts at: 513 1065 2004 3252 3462 4807 5578 5613 6109
Size: 552 939 1248 210 1345 771 35 496
Cuts at: 6109 6341 513
Size: 232 3074
Fragments arranged by size:
3074 1345 1248 939 771 552 496 232
210 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5859 6929 6946 7099 7284 7729 325
Size: 1255 4279 1070 17 153 185 445 1498
Fragments arranged by size:
4279 1498 1255 1070 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2198 3047 4530
Size: 274 80 33 51 1443 221 849 1483
Cuts at: 4530 4556 4747 5441 5947 7148 8860 96
Size: 26 191 694 506 1201 1712 138
Fragments arranged by size:
1712 1483 1443 1201 849 694 506 274
221 191 138 80 51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2529 2561 5593 5625 8862 8894 2529
Size: 32 3032 32 3237 32 2537
Fragments arranged by size:
3237 3032 2537 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8902
XbaI T'CTAG_A
Cuts at: 3295 4039 6238 3295
Size: 744 2199 5959
Fragments arranged by size:
5959 2199 744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5761 5761
Size: 8902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8902
XmaI C'CCGG_G
Cuts at: 1870 4152 6227 1870
Size: 2282 2075 4545
Fragments arranged by size:
4545 2282 2075
XmnI GAAnn'nnTTC
Cuts at: 3192 4260 7031 3192
Size: 1068 2771 5063
Fragments arranged by size:
5063 2771 1068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6710 276
Size: 53 83 186 936 5176 2468
Fragments arranged by size:
5176 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BplI BsiWI BsmBI BstZ17I Bsu36I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI
PspXI RsrII SacII SanDI SfiI SrfI SwaI