Restriction map for
pFN21AA1448
(Circular) (Six-base) MAPSORT of: pFN21AA1448.seq Check: 4405 from: 1 to: 8164
pFN21AA1448 8164 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3233 4380 4422 4759
Size: 53 83 186 936 1697 1147 42 337
Cuts at: 4759 5974 278
Size: 1215 2468
Fragments arranged by size:
2468 1697 1215 1147 936 337 186 83
53 42
AccI GT'mk_AC
Cuts at: 1937 3263 5507 1937
Size: 1326 2244 4594
Fragments arranged by size:
4594 2244 1326
Acc65I G'GTAC_C
Cuts at: 5485 5485
Size: 8164
AclI AA'CG_TT
Cuts at: 1563 3292 6291 6664 1563
Size: 1729 2999 373 3063
Fragments arranged by size:
3063 2999 1729 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2137 3143 4243 6227 7611 7879 1632
Size: 505 1006 1100 1984 1384 268 1917
Fragments arranged by size:
1984 1917 1384 1100 1006 505 268
AfeI AGC'GCT
Cuts at: 3671 3671
Size: 8164
AflII C'TTAA_G
Cuts at: 820 1017 4543 820
Size: 197 3526 4441
Fragments arranged by size:
4441 3526 197
AflIII A'CryG_T
Cuts at: 1203 4711 7063 8040 1203
Size: 3508 2352 977 1327
Fragments arranged by size:
3508 2352 1327 977
AgeI A'CCGG_T
Cuts at: 6995 6995
Size: 8164
AhdI GACnn_n'nnGTC
Cuts at: 4018 6893 4018
Size: 2875 5289
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2261 2319 1184
Size: 1077 58 7029
Fragments arranged by size:
7029 1077 58
AlwNI CAG_nnn'CTG
Cuts at: 3148 3554 3979 5382 5907 7479 3148
Size: 406 425 1403 525 1572 3833
Fragments arranged by size:
3833 1572 1403 525 425 406
ApaLI G'TGCA_C
Cuts at: 6221 7377 6221
Size: 1156 7008
ApoI r'AATT_y
Cuts at: 1493 2448 2938 4212 4782 5473 5699 5851 7960
Size: 955 490 1274 570 691 226 152 2109
Cuts at: 7960 1493
Size: 1697
Fragments arranged by size:
2109 1697 1274 955 691 570 490 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2817 5118 6718 160
Size: 872 1785 2301 1600 1606
Fragments arranged by size:
2301 1785 1606 1600 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8164
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 5489 1870
Size: 73 112 3434 4545
Fragments arranged by size:
4545 3434 112 73
AvrII C'CTAG_G
Cuts at: 3433 3433
Size: 8164
BamHI G'GATC_C
Cuts at: 4735 5047 5494 5861 4735
Size: 312 447 367 7038
Fragments arranged by size:
7038 447 367 312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2780 3561 5485 6940 618
Size: 325 217 627 993 781 1924 1455 1842
Fragments arranged by size:
1924 1842 1455 993 781 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2984 3589 3663 5069 5483 1391
Size: 1593 605 74 1406 414 4072
Fragments arranged by size:
4072 1593 1406 605 414 74
BbeI G_GCGC'C
Cuts at: 3565 3565
Size: 8164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3489 3809 4422 928
Size: 971 1590 320 613 4670
Fragments arranged by size:
4670 1590 971 613 320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4811 4845 6355 6389 1903
Size: 34 2874 34 1510 34 3678
Fragments arranged by size:
3678 2874 1510 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4378 6056 7266 4378
Size: 1678 1210 5276
Fragments arranged by size:
5276 1678 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8164
BfrBI ATG'CAT
Cuts at: 2082 2082
Size: 8164
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2352 6775 136
Size: 107 122 71 631 605 680 4423 1525
Fragments arranged by size:
4423 1525 680 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3924 3955 4204 8159 3924
Size: 31 249 3955 3929
Fragments arranged by size:
3955 3929 249 31
BlpI GC'TnA_GC
Cuts at: 3555 3578 3792 5581 3555
Size: 23 214 1789 6138
Fragments arranged by size:
6138 1789 214 23
Bme1580I G_kGCm'C
Cuts at: 1790 6225 7381 1790
Size: 4435 1156 2573
Fragments arranged by size:
4435 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4751 1318
Size: 3433 4731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4440 5047 5895
Size: 416 53 83 399 24 3023 607 848
Cuts at: 5895 6853 442
Size: 958 1753
Fragments arranged by size:
3023 1753 958 848 607 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3603 3841 4369 3603
Size: 238 528 7398
Fragments arranged by size:
7398 528 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3291 4378 5340 6824 1239
Size: 2052 1087 962 1484 2579
Fragments arranged by size:
2579 2052 1484 1087 962
Bpu10I CC'TnA_GC
Cuts at: 3141 3724 4967 3141
Size: 583 1243 6338
Fragments arranged by size:
6338 1243 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 4445 5055 6289 7154 7452 4192
Size: 253 610 1234 865 298 4904
Fragments arranged by size:
4904 1234 865 610 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 2181 2696 3812 6827 882
Size: 617 555 127 515 1116 3015 2219
Fragments arranged by size:
3015 2219 1116 617 555 515 127
BsaAI yAC'GTr
Cuts at: 493 1204 3685 5955 493
Size: 711 2481 2270 2702
Fragments arranged by size:
2702 2481 2270 711
BsaBI GATnn'nnATC
Cuts at: 8158 8158
Size: 8164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3230 3562 4377 4419
Size: 53 83 186 936 1697 332 815 42
Cuts at: 4419 4756 5971 6353 6988 275
Size: 337 1215 382 635 1451
Fragments arranged by size:
1697 1451 1215 936 815 635 382 337
332 186 83 53 42
BsaWI w'CCGG_w
Cuts at: 3615 4869 6597 6995 7269 7416 7827 3615
Size: 1254 1728 398 274 147 411 3952
Fragments arranged by size:
3952 1728 1254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 2531 2561 1178
Size: 30 165 30 601 30 497 30 6781
Fragments arranged by size:
6781 601 497 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3122 3288 4544 5428 1186
Size: 1936 166 1256 884 3922
Fragments arranged by size:
3922 1936 1256 884 166
BseYI C'CCAG_C
Cuts at: 2597 3705 4073 4719 5375 7367 7863 2597
Size: 1108 368 646 656 1992 496 2898
Fragments arranged by size:
2898 1992 1108 656 646 496 368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7591
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5614 6375 7403 1540
Size: 275 186 3613 761 1028 2301
Fragments arranged by size:
3613 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3017 3142 6225 6310 7381 3017
Size: 125 3083 85 1071 3800
Fragments arranged by size:
3800 3083 1071 125 85
BsmI GAATG_Cn'
Cuts at: 5675 5768 5675
Size: 93 8071
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2984 3017 3142 3589 3663 5069 5483
Size: 399 1194 33 125 447 74 1406 414
Cuts at: 5483 6225 6310 7381 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1406 1194 1071 742 447 414 399
125 85 74 33
BspEI T'CCGG_A
Cuts at: 3615 7827 3615
Size: 4212 3952
BspHI T'CATG_A
Cuts at: 6053 7783 6053
Size: 1730 6434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5519 844
Size: 4675 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3559 3582 4348 6051 1394
Size: 429 1736 23 766 1703 3507
Fragments arranged by size:
3507 1736 1703 766 429 23
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 2970 3061 3315 5344 6659 6833
Size: 1183 1329 393 91 254 2029 1315 174
Cuts at: 6833 65
Size: 1396
Fragments arranged by size:
2029 1396 1329 1315 1183 393 254 174
91
BsrFI r'CCGG_y
Cuts at: 1700 1810 6808 6995 1700
Size: 110 4998 187 2869
Fragments arranged by size:
4998 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2223 5254 96
Size: 2127 3031 3006
Fragments arranged by size:
3031 3006 2127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8164
BssSI C'ACGA_G
Cuts at: 3786 6224 7236 3786
Size: 2438 1012 4714
Fragments arranged by size:
4714 2438 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2969 3035 794
Size: 2175 66 5923
Fragments arranged by size:
5923 2175 66
BstBI TT'CG_AA
Cuts at: 6968 6968
Size: 8164
BstEII G'GTnAC_C
Cuts at: 1184 5237 1184
Size: 4053 4111
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 3373 2617
Size: 756 7408
BstYI r'GATC_y
Cuts at: 1972 2194 2542 2746 3924 3955 4204 4589 4735
Size: 222 348 204 1178 31 249 385 146
Cuts at: 4735 5047 5494 5861 6246 6263 7715 7801 7813
Size: 312 447 367 385 17 1452 86 12
Cuts at: 7813 8159 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1178 447 385 385 367 348
346 312 249 222 204 146 86 31
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2985 3171 5060 5211 5405 513
Size: 552 1920 186 1889 151 194 3272
Fragments arranged by size:
3272 1920 1889 552 194 186 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7425
Fragments arranged by size:
7425 683 56
BtsI GCAGTG_nn'
Cuts at: 4123 4371 5244 5680 6474 6494 4123
Size: 248 873 436 794 20 5793
Fragments arranged by size:
5793 873 794 436 248 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8129
DraI TTT'AAA
Cuts at: 3297 5468 3297
Size: 2171 5993
DraIII CAC_nnn'GTG
Cuts at: 2662 2662
Size: 8164
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4755 7171 809
Size: 507 3439 2416 1802
Fragments arranged by size:
3439 2416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2859 3541 4290
Size: 54 1425 50 275 18 1029 682 749
Cuts at: 4290 4368 4425 5173 5611 6500 8129 8
Size: 78 57 748 438 889 1629 43
Fragments arranged by size:
1629 1425 1029 889 749 748 682 438
275 78 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5611 1537
Size: 275 3799 4090
Fragments arranged by size:
4090 3799 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3336 3594 4023 5004 6094 1353
Size: 1983 258 429 981 1090 3423
Fragments arranged by size:
3423 1983 1090 981 429 258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6751 7125 7271 387
Size: 1064 138 5162 374 146 1280
Fragments arranged by size:
5162 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2137 3143 3291 4243 4378 5340 6227
Size: 393 505 1006 148 952 135 962 887
Cuts at: 6227 6824 7611 7879 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1006 962 952 887 787 597 505
393 268 148 135
EcoO109I rG'GnC_Cy
Cuts at: 2043 3073 3127 3145 3177 2043
Size: 1030 54 18 32 7030
Fragments arranged by size:
7030 1030 54 32 18
EcoRI G'AATT_C
Cuts at: 5473 5851 5473
Size: 378 7786
EcoRV GAT'ATC
Cuts at: 2992 2992
Size: 8164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3365 3974 4897 3365
Size: 609 923 6632
Fragments arranged by size:
6632 923 609
FspI TGC'GCA
Cuts at: 6670 6670
Size: 8164
HaeII r_GCGC'y
Cuts at: 1678 3565 3673 4171 5278 7311 1678
Size: 1887 108 498 1107 2033 2531
Fragments arranged by size:
2531 2033 1887 1107 498 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2259 2291 2531 2563 3448
Size: 32 135 32 687 32 240 32 885
Cuts at: 3448 3480 4004 4036 4068 4576 4608 6805 6837
Size: 32 524 32 32 508 32 2197 32
Cuts at: 6837 6879 6911 1373
Size: 42 32 2626
Fragments arranged by size:
2626 2197 885 687 524 508 240 135
42 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3472 5508 5754 669
Size: 988 281 1534 2036 246 3079
Fragments arranged by size:
3079 2036 1534 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3347 748
Size: 2599 5565
HpaI GTT'AAC
Cuts at: 3472 5754 3472
Size: 2282 5882
KasI G'GCGC_C
Cuts at: 3561 3561
Size: 8164
KpnI G_GTAC'C
Cuts at: 5489 5489
Size: 8164
MfeI C'AATT_G
Cuts at: 5763 5763
Size: 8164
MluI A'CGCG_T
Cuts at: 8040 8040
Size: 8164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5129 7278 7462 1305
Size: 3824 2149 184 2007
Fragments arranged by size:
3824 2149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3543 4292 4370 4427 5175
Size: 54 1425 343 1711 749 78 57 748
Cuts at: 5175 10
Size: 2999
Fragments arranged by size:
2999 1711 1425 749 748 343 78 57
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2368 2697 3371 6122 6481 6640
Size: 648 924 278 329 674 2751 359 159
Cuts at: 6640 518
Size: 2042
Fragments arranged by size:
2751 2042 924 674 648 359 329 278
159
MspA1I CmG'CkG
Cuts at: 1606 2987 3636 3718 4723 5213 5407 5580 6257
Size: 1381 649 82 1005 490 194 173 677
Cuts at: 6257 7405 7650 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1381 1148 1005 677 649 490 245
194 173 82
NarI GG'CG_CC
Cuts at: 3562 3562
Size: 8164
NcoI C'CATG_G
Cuts at: 513 1065 3171 5060 513
Size: 552 2106 1889 3617
Fragments arranged by size:
3617 2106 1889 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8164
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8164
NotI GC'GGCC_GC
Cuts at: 5611 5611
Size: 8164
NsiI A_TGCA'T
Cuts at: 2084 2084
Size: 8164
NspI r_CATG'y
Cuts at: 1132 1229 5522 7067 1132
Size: 97 4293 1545 2229
Fragments arranged by size:
4293 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8164
PciI A'CATG_T
Cuts at: 7063 7063
Size: 8164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2525 4118 1108
Size: 1417 1593 5154
Fragments arranged by size:
5154 1593 1417
PfoI T'CCnGG_A
Cuts at: 4355 4355
Size: 8164
PmeI GTTT'AAAC
Cuts at: 5468 5468
Size: 8164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6233 6265 7746 7778 6233
Size: 32 1481 32 6619
Fragments arranged by size:
6619 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3073 3127 3177 3073
Size: 54 50 8060
Fragments arranged by size:
8060 54 50
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8164
PsiI TTA'TAA
Cuts at: 5313 5734 5313
Size: 421 7743
PstI C_TGCA'G
Cuts at: 830 5516 830
Size: 4686 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8164
PvuII CAG'CTG
Cuts at: 3718 4723 3718
Size: 1005 7159
SacII CC_GC'GG
Cuts at: 2988 5214 5408 2988
Size: 2226 194 5744
Fragments arranged by size:
5744 2226 194
SalI G'TCGA_C
Cuts at: 1936 5506 1936
Size: 3570 4594
SanDI GG'GwC_CC
Cuts at: 3177 3177
Size: 8164
SapI GCTCTTCn'nnn_
Cuts at: 3594 3594
Size: 8164
SbfI CC_TGCA'GG
Cuts at: 5516 5516
Size: 8164
ScaI AGT'ACT
Cuts at: 6412 8124 6412
Size: 1712 6452
SfcI C'TryA_G
Cuts at: 826 1045 2427 2902 3911 5383 5512 6647 7328
Size: 219 1382 475 1009 1472 129 1135 681
Cuts at: 7328 7519 826
Size: 191 1471
Fragments arranged by size:
1472 1471 1382 1135 1009 681 475 219
191 129
SfoI GGC'GCC
Cuts at: 3563 3563
Size: 8164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8164
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8164
SmaI CCC'GGG
Cuts at: 1872 5491 1872
Size: 3619 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2055 4171 4460 4543 5070 6268
Size: 197 926 112 2116 289 83 527 1198
Cuts at: 6268 7169 7431 820
Size: 901 262 1553
Fragments arranged by size:
2116 1553 1198 926 901 527 289 262
197 112 83
SnaBI TAC'GTA
Cuts at: 493 3685 5955 493
Size: 3192 2270 2702
Fragments arranged by size:
3192 2702 2270
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8164
SphI G_CATG'C
Cuts at: 1132 5522 1132
Size: 4390 3774
SspI AAT'ATT
Cuts at: 5 52 2815 6088 5
Size: 47 2763 3273 2081
Fragments arranged by size:
3273 2763 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8164
StyI C'CwwG_G
Cuts at: 513 1065 2856 3171 3433 5060 5603 513
Size: 552 1791 315 262 1627 543 3074
Fragments arranged by size:
3074 1791 1627 552 543 315 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2532 2696 5446 6191 6208 6361 6546
Size: 1255 952 164 2750 745 17 153 185
Cuts at: 6546 6991 325
Size: 445 1498
Fragments arranged by size:
2750 1498 1255 952 745 445 185 164
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 2914 5254
Size: 274 80 33 51 1443 246 691 2340
Cuts at: 5254 6410 8122 96
Size: 1156 1712 138
Fragments arranged by size:
2340 1712 1443 1156 691 274 246 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 2520 2552 4114 4146 8124 8156 2004
Size: 32 484 32 1562 32 3978 32 2012
Fragments arranged by size:
3978 2012 1562 484 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8164
XbaI T'CTAG_A
Cuts at: 5500 5500
Size: 8164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3085 4727 3085
Size: 1642 6522
XhoI C'TCGA_G
Cuts at: 1943 2055 1943
Size: 112 8052
XmaI C'CCGG_G
Cuts at: 1870 5489 1870
Size: 3619 4545
XmnI GAAnn'nnTTC
Cuts at: 5158 6293 5158
Size: 1135 7029
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3231 4378 4420 4757
Size: 53 83 186 936 1697 1147 42 337
Cuts at: 4757 5972 276
Size: 1215 2468
Fragments arranged by size:
2468 1697 1215 1147 936 337 186 83
53 42
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbvCI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SexAI SfiI SrfI SwaI