Restriction map for
pFN21AA1434
(Circular) (Six-base) MAPSORT of: pFN21AA1434.seq Check: 1249 from: 1 to: 6721
pFN21AA1434 6721 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3711 3914 4784 6168 6436 1632
Size: 2079 203 870 1384 268 1917
Fragments arranged by size:
2079 1917 1384 870 268 203
AflII C'TTAA_G
Cuts at: 820 1017 3326 3914 820
Size: 197 2309 588 3627
Fragments arranged by size:
3627 2309 588 197
AflIII A'CryG_T
Cuts at: 1203 2387 2755 5620 6597 1203
Size: 1184 368 2865 977 1327
Fragments arranged by size:
2865 1327 1184 977 368
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 5450 5450
Size: 6721
AleI CACnn'nnGTG
Cuts at: 1166 2355 3239 1166
Size: 1189 884 4648
Fragments arranged by size:
4648 1189 884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3544 1184
Size: 2360 4361
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 2178
Size: 32 6689
AlwNI CAG_nnn'CTG
Cuts at: 4464 6036 4464
Size: 1572 5149
ApaLI G'TGCA_C
Cuts at: 4778 5934 4778
Size: 1156 5565
ApoI r'AATT_y
Cuts at: 1493 2814 2842 3077 3155 3186 3292 3481 4030
Size: 1321 28 235 78 31 106 189 549
Cuts at: 4030 4256 4408 6517 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1321 549 235 226 189 152
106 78 31 28
AseI AT'TA_AT
Cuts at: 160 1032 5275 160
Size: 872 4243 1606
Fragments arranged by size:
4243 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 4046 1870
Size: 73 2103 4545
Fragments arranged by size:
4545 2103 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2896 2929 2896
Size: 33 6688
BamHI G'GATC_C
Cuts at: 4051 4418 4051
Size: 367 6354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4042 5497 618
Size: 325 217 627 2255 1455 1842
Fragments arranged by size:
2255 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4040 1391
Size: 2649 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3699 928
Size: 971 1800 3950
Fragments arranged by size:
3950 1800 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2621 2655 4912 4946 1903
Size: 34 684 34 2257 34 3678
Fragments arranged by size:
3678 2257 684 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2897 4613 5823 2897
Size: 1716 1210 3795
Fragments arranged by size:
3795 1716 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3008 5332 136
Size: 107 122 71 631 605 1336 2324 1525
Fragments arranged by size:
2324 1525 1336 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2713 3620 6716 2713
Size: 907 3096 2718
Fragments arranged by size:
3096 2718 907
BlpI GC'TnA_GC
Cuts at: 4138 4138
Size: 6721
Bme1580I G_kGCm'C
Cuts at: 1790 3111 4782 5938 1790
Size: 1321 1671 1156 2573
Fragments arranged by size:
2573 1671 1321 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3680 4452 5410
Size: 416 53 83 399 24 2263 772 958
Cuts at: 5410 442
Size: 1753
Fragments arranged by size:
2263 1753 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 3203 5381 1239
Size: 1283 681 2178 2579
Fragments arranged by size:
2579 2178 1283 681
Bpu10I CC'TnA_GC
Cuts at: 3735 3735
Size: 6721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3564 4846 5711 6009 3564
Size: 1282 865 298 4276
Fragments arranged by size:
4276 1282 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5384 882
Size: 617 3885 2219
Fragments arranged by size:
3885 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2728 4512 493
Size: 711 1524 1784 2702
Fragments arranged by size:
2702 1784 1524 711
BsaBI GATnn'nnATC
Cuts at: 3165 6715 3165
Size: 3550 3171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4528 4910 5545 275
Size: 53 83 186 936 2995 382 635 1451
Fragments arranged by size:
2995 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5154 5552 5826 5973 6384 5154
Size: 398 274 147 411 5491
Fragments arranged by size:
5491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2178 2208 1178
Size: 30 165 30 775 30 5691
Fragments arranged by size:
5691 775 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 3508 1186
Size: 1013 1309 4399
Fragments arranged by size:
4399 1309 1013
BseYI C'CCAG_C
Cuts at: 3003 3756 5924 6420 3003
Size: 753 2168 496 3304
Fragments arranged by size:
3304 2168 753 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2617 3000 3084 3231 1163
Size: 573 881 383 84 147 4653
Fragments arranged by size:
4653 881 573 383 147 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4171 4932 5960 1540
Size: 275 186 2170 761 1028 2301
Fragments arranged by size:
2301 2170 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2727 4782 4867 5938 2727
Size: 2055 85 1071 3510
Fragments arranged by size:
3510 2055 1071 85
BsmI GAATG_Cn'
Cuts at: 2602 4021 4232 4325 2602
Size: 1419 211 93 4998
Fragments arranged by size:
4998 1419 211 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2727 3111 4040 4782 4867 5938 1391
Size: 399 937 384 929 742 85 1071 2174
Fragments arranged by size:
2174 1071 937 929 742 399 384 85
BspEI T'CCGG_A
Cuts at: 6384 6384
Size: 6721
BspHI T'CATG_A
Cuts at: 2832 3121 4610 6340 2832
Size: 289 1489 1730 3213
Fragments arranged by size:
3213 1730 1489 289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4076 844
Size: 3232 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4608 1394
Size: 429 2785 3507
Fragments arranged by size:
3507 2785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 3636 3648 5216 5390 65
Size: 1183 1539 849 12 1568 174 1396
Fragments arranged by size:
1568 1539 1396 1183 849 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 5365 5552 1700
Size: 110 3555 187 2869
Fragments arranged by size:
3555 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3682 96
Size: 3586 3135
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 4781 5793 4781
Size: 1012 5709
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3904 794
Size: 3110 3611
BstBI TT'CG_AA
Cuts at: 2706 5525 2706
Size: 2819 3902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 2560 2560
Size: 6721
BstYI r'GATC_y
Cuts at: 1972 2713 3384 3620 4051 4418 4803 4820 6272
Size: 741 671 236 431 367 385 17 1452
Cuts at: 6272 6358 6370 6716 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 741 671 431 385 367 346
236 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3111 513
Size: 552 2046 4123
Fragments arranged by size:
4123 2046 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5982
Fragments arranged by size:
5982 683 56
BtsI GCAGTG_nn'
Cuts at: 4237 5031 5051 4237
Size: 794 20 5907
Fragments arranged by size:
5907 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6686
DraI TTT'AAA
Cuts at: 3465 4025 3465
Size: 560 6161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5728 809
Size: 507 4412 1802
Fragments arranged by size:
4412 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2968 4168 5057
Size: 54 1425 50 275 18 1138 1200 889
Cuts at: 5057 6686 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1200 1138 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4168 1537
Size: 275 2356 4090
Fragments arranged by size:
4090 2356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2829 3485 4651 1353
Size: 774 702 656 1166 3423
Fragments arranged by size:
3423 1166 774 702 656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5308 5682 5828 387
Size: 1064 138 3719 374 146 1280
Fragments arranged by size:
3719 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 3203 3711 3914 4784 5381 6168
Size: 393 890 681 508 203 870 597 787
Cuts at: 6168 6436 1239
Size: 268 1524
Fragments arranged by size:
1524 890 870 787 681 597 508 393
268 203
EcoNI CCTnn'n_nnAGG
Cuts at: 3095 3896 3095
Size: 801 5920
EcoRI G'AATT_C
Cuts at: 2814 4030 4408 2814
Size: 1216 378 5127
Fragments arranged by size:
5127 1216 378
EcoRV GAT'ATC
Cuts at: 2568 3979 2568
Size: 1411 5310
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 5868 1678
Size: 4190 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2510 2542 2906
Size: 32 135 32 427 32 479 32 364
Cuts at: 2906 2938 3605 3637 5362 5394 5436 5468 1373
Size: 32 667 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 667 479 427 364 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2875 4065 4311 669
Size: 988 281 937 1190 246 3079
Fragments arranged by size:
3079 1190 988 937 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6721
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 3881 4320 3881
Size: 439 6282
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2624 5835 6019 1305
Size: 1319 3211 184 2007
Fragments arranged by size:
3211 2007 1319 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2970 10
Size: 54 1425 343 1138 3761
Fragments arranged by size:
3761 1425 1138 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 2976 3239 3641 3961 3987 4679
Size: 648 1189 621 263 402 320 26 692
Cuts at: 4679 5038 5197 518
Size: 359 159 2042
Fragments arranged by size:
2042 1189 692 648 621 402 359 320
263 159 26
MspA1I CmG'CkG
Cuts at: 1606 3007 4137 4814 5962 6207 1606
Size: 1401 1130 677 1148 245 2120
Fragments arranged by size:
2120 1401 1148 1130 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6169
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6721
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 2155 2391 2759 4079 5624 1132
Size: 97 926 236 368 1320 1545 2229
Fragments arranged by size:
2229 1545 1320 926 368 236 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6721
PciI A'CATG_T
Cuts at: 2387 2755 5620 2387
Size: 368 2865 3488
Fragments arranged by size:
3488 2865 368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2940 1108
Size: 1832 4889
PfoI T'CCnGG_A
Cuts at: 2749 2846 2749
Size: 97 6624
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2178 2210 4790 4822 6303 6335 2178
Size: 32 2580 32 1481 32 2564
Fragments arranged by size:
2580 2564 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6721
PsiI TTA'TAA
Cuts at: 4291 4291
Size: 6721
PstI C_TGCA'G
Cuts at: 830 4073 830
Size: 3243 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
PvuII CAG'CTG
Cuts at: 3007 3007
Size: 6721
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SapI GCTCTTCn'nnn_
Cuts at: 2127 2127
Size: 6721
SbfI CC_TGCA'GG
Cuts at: 4073 4073
Size: 6721
ScaI AGT'ACT
Cuts at: 4969 6681 4969
Size: 1712 5009
SfcI C'TryA_G
Cuts at: 826 1045 4069 5204 5885 6076 826
Size: 219 3024 1135 681 191 1471
Fragments arranged by size:
3024 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 4048 1872
Size: 2176 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3326 3579 3914 4825 5726 5988
Size: 197 926 1383 253 335 911 901 262
Cuts at: 5988 820
Size: 1553
Fragments arranged by size:
1553 1383 926 911 901 335 262 253
197
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 3742 152
Size: 3590 3131
SphI G_CATG'C
Cuts at: 1132 2155 4079 1132
Size: 1023 1924 3774
Fragments arranged by size:
3774 1924 1023
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 2499 1517
Size: 982 5739
StyI C'CwwG_G
Cuts at: 513 1065 4160 513
Size: 552 3095 3074
Fragments arranged by size:
3095 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4748 4765 4918 5103 5548 325
Size: 1255 3168 17 153 185 445 1498
Fragments arranged by size:
3168 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2173 2536 2652
Size: 274 80 33 51 1443 196 363 116
Cuts at: 2652 3682 4967 6679 96
Size: 1030 1285 1712 138
Fragments arranged by size:
1712 1443 1285 1030 363 274 196 138
116 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 3392 3424 6681 6713 2534
Size: 32 826 32 3257 32 2542
Fragments arranged by size:
3257 2542 826 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 2468 4057 2468
Size: 1589 5132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3398 3398
Size: 6721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 4046 1870
Size: 2176 4545
XmnI GAAnn'nnTTC
Cuts at: 4850 4850
Size: 6721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI