Restriction map for
pFN21AA1426
(Circular) (Six-base) MAPSORT of: pFN21AA1426.seq Check: 3974 from: 1 to: 7900
pFN21AA1426 7900 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3307 4047 3307
Size: 740 7160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4608 5710 278
Size: 53 83 186 936 3072 1102 2468
Fragments arranged by size:
3072 2468 1102 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3465 5243 1937
Size: 1528 1778 4594
Fragments arranged by size:
4594 1778 1528
Acc65I G'GTAC_C
Cuts at: 4848 5221 4848
Size: 373 7527
AclI AA'CG_TT
Cuts at: 1563 4104 6027 6400 1563
Size: 2541 1923 373 3063
Fragments arranged by size:
3063 2541 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2303 3614 4332 5963 7347 7615 1632
Size: 491 180 1311 718 1631 1384 268 1917
Fragments arranged by size:
1917 1631 1384 1311 718 491 268 180
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7703
AflIII A'CryG_T
Cuts at: 1203 6799 7776 1203
Size: 5596 977 1327
Fragments arranged by size:
5596 1327 977
AgeI A'CCGG_T
Cuts at: 6731 6731
Size: 7900
AhdI GACnn_n'nnGTC
Cuts at: 6629 6629
Size: 7900
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7900
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2424 4979 1184
Size: 1240 2555 4105
Fragments arranged by size:
4105 2555 1240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 7868
AlwNI CAG_nnn'CTG
Cuts at: 2122 2734 3973 5643 7215 2122
Size: 612 1239 1670 1572 2807
Fragments arranged by size:
2807 1670 1572 1239 612
ApaI G_GGCC'C
Cuts at: 2281 2395 3522 2281
Size: 114 1127 6659
Fragments arranged by size:
6659 1127 114
ApaLI G'TGCA_C
Cuts at: 2639 2663 5957 7113 2639
Size: 24 3294 1156 3426
Fragments arranged by size:
3426 3294 1156 24
ApoI r'AATT_y
Cuts at: 1493 2471 5209 5435 5587 7696 1493
Size: 978 2738 226 152 2109 1697
Fragments arranged by size:
2738 2109 1697 978 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6454 160
Size: 872 5422 1606
Fragments arranged by size:
5422 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7900
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 2522 2763 2876 2899 2997 3113
Size: 73 151 428 241 113 23 98 116
Cuts at: 3113 3241 3254 4372 5225 1870
Size: 128 13 1118 853 4545
Fragments arranged by size:
4545 1118 853 428 241 151 128 116
113 98 73 23 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4840 4873 4840
Size: 33 7867
BamHI G'GATC_C
Cuts at: 5230 5597 5230
Size: 367 7533
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2937 3835 4035 4364 4848
Size: 325 217 627 1150 898 200 329 484
Cuts at: 4848 5221 6676 618
Size: 373 1455 1842
Fragments arranged by size:
1842 1455 1150 898 627 484 373 329
325 217 200
BanII G_rGCy'C
Cuts at: 1391 2281 2395 2849 3057 3261 3312 3329 3522
Size: 890 114 454 208 204 51 17 193
Cuts at: 3522 4433 4499 4580 5219 1391
Size: 911 66 81 639 4072
Fragments arranged by size:
4072 911 890 639 454 208 204 193
114 81 66 51 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2522 2972 4164 4338 928
Size: 971 623 450 1192 174 4490
Fragments arranged by size:
4490 1192 971 623 450 174
BbvCI CC'TCA_GC
Cuts at: 2796 3581 2796
Size: 785 7115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 6091 6125 1903
Size: 34 1946 34 2174 34 3678
Fragments arranged by size:
3678 2174 1946 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5792 7002 5792
Size: 1210 6690
BclI T'GATC_A
Cuts at: 1548 2080 1548
Size: 532 7368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6511 136
Size: 107 122 71 631 605 4839 1525
Fragments arranged by size:
4839 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7895 7895
Size: 7900
BlpI GC'TnA_GC
Cuts at: 3402 4877 5130 5317 3402
Size: 1475 253 187 5985
Fragments arranged by size:
5985 1475 253 187
Bme1580I G_kGCm'C
Cuts at: 1790 2281 2395 2643 2667 2759 3522 4212 5961
Size: 491 114 248 24 92 763 690 1749
Cuts at: 5961 7117 1790
Size: 1156 2573
Fragments arranged by size:
2573 1749 1156 763 690 491 248 114
92 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7900
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2958 3933 4152
Size: 416 53 83 399 24 1541 975 219
Cuts at: 4152 4837 4848 5631 6589 442
Size: 685 11 783 958 1753
Fragments arranged by size:
1753 1541 975 958 783 685 416 399
219 83 53 24 11
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7900
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3834 4430 3834
Size: 596 7304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2443 3065 3296 3521 3832 4523 4564 4731
Size: 1204 622 231 225 311 691 41 167
Cuts at: 4731 6560 1239
Size: 1829 2579
Fragments arranged by size:
2579 1829 1204 691 622 311 231 225
167 41
Bpu10I CC'TnA_GC
Cuts at: 2796 2894 3581 4464 2796
Size: 98 687 883 6232
Fragments arranged by size:
6232 883 687 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2329 2981 6025 6890 7188 2329
Size: 652 3044 865 298 3041
Fragments arranged by size:
3044 3041 865 652 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2461 6563 882
Size: 617 962 4102 2219
Fragments arranged by size:
4102 2219 962 617
BsaAI yAC'GTr
Cuts at: 493 1204 2713 5691 493
Size: 711 1509 2978 2702
Fragments arranged by size:
2978 2702 1509 711
BsaBI GATnn'nnATC
Cuts at: 4706 7894 4706
Size: 3188 4712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2706 2835 3692 4605
Size: 53 83 186 936 1173 129 857 913
Cuts at: 4605 5707 6089 6724 275
Size: 1102 382 635 1451
Fragments arranged by size:
1451 1173 1102 936 913 857 635 382
186 129 83 53
BsaWI w'CCGG_w
Cuts at: 2849 4715 6333 6731 7005 7152 7563 2849
Size: 1866 1618 398 274 147 411 3186
Fragments arranged by size:
3186 1866 1618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 3668 3698 3737
Size: 30 165 30 1131 30 1104 30 39
Cuts at: 3737 3767 1178
Size: 30 5311
Fragments arranged by size:
5311 1131 1104 165 39 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 2919 3320 3431 3569 3692 3790 4861
Size: 1354 379 401 111 138 123 98 1071
Cuts at: 4861 1186
Size: 4225
Fragments arranged by size:
4225 1354 1071 401 379 138 123 111
98
BseYI C'CCAG_C
Cuts at: 2722 3485 3514 3782 4806 4973 5049 7103 7599
Size: 763 29 268 1024 167 76 2054 496
Cuts at: 7599 2722
Size: 3023
Fragments arranged by size:
3023 2054 1024 763 496 268 167 76
29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2564 2641 3774 1163
Size: 573 684 144 77 1133 5289
Fragments arranged by size:
5289 1133 684 573 144 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2683 3526 4202 4206 5350 6111
Size: 275 186 682 843 676 4 1144 761
Cuts at: 6111 7139 1540
Size: 1028 2301
Fragments arranged by size:
2301 1144 1028 843 761 682 676 275
186 4
BsiHKAI G_wGCw'C
Cuts at: 2643 2667 2849 3057 3312 4580 5961 6046 7117
Size: 24 182 208 255 1268 1381 85 1071
Cuts at: 7117 2643
Size: 3426
Fragments arranged by size:
3426 1381 1268 1071 255 208 182 85
24
BsmI GAATG_Cn'
Cuts at: 2191 5411 5504 2191
Size: 3220 93 4587
Fragments arranged by size:
4587 3220 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3351 4612 3351
Size: 1261 6639
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 2395 2643 2667 2759 2849 3057
Size: 399 491 114 248 24 92 90 208
Cuts at: 3057 3261 3312 3329 3522 4212 4433 4499 4580
Size: 204 51 17 193 690 221 66 81
Cuts at: 4580 5219 5961 6046 7117 1391
Size: 639 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 690 639 491 399 248
221 208 204 193 114 92 90 85
81 66 51 24 17
BspEI T'CCGG_A
Cuts at: 2849 7563 2849
Size: 4714 3186
BspHI T'CATG_A
Cuts at: 3471 5789 7519 3471
Size: 2318 1730 3852
Fragments arranged by size:
3852 2318 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2014 2259 3307 3438 4047 5255 844
Size: 1170 245 1048 131 609 1208 3489
Fragments arranged by size:
3489 1208 1170 1048 609 245 131
BsrBI CCG'CTC
Cuts at: 1394 1823 2530 2790 5787 1394
Size: 429 707 260 2997 3507
Fragments arranged by size:
3507 2997 707 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 3868 6395 6569 65
Size: 1183 2620 2527 174 1396
Fragments arranged by size:
2620 2527 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2682 3393 4032 4277 6544 6731 1700
Size: 110 872 711 639 245 2267 187 2869
Fragments arranged by size:
2869 2267 872 711 639 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 2603 96
Size: 2507 5393
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7900
BssSI C'ACGA_G
Cuts at: 4234 5960 6972 4234
Size: 1726 1012 5162
Fragments arranged by size:
5162 1726 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7900
BstBI TT'CG_AA
Cuts at: 6704 6704
Size: 7900
BstEII G'GTnAC_C
Cuts at: 1184 3709 1184
Size: 2525 5375
BstXI CCAn_nnnn'nTGG
Cuts at: 2948 3397 4981 5020 2948
Size: 449 1584 39 5828
Fragments arranged by size:
5828 1584 449 39
BstYI r'GATC_y
Cuts at: 1972 2730 5230 5597 5982 5999 7451 7537 7549
Size: 758 2500 367 385 17 1452 86 12
Cuts at: 7549 7895 1972
Size: 346 1977
Fragments arranged by size:
2500 1977 1452 758 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2426 3802 4644 2426
Size: 1376 842 5682
Fragments arranged by size:
5682 1376 842
BtgI C'CryG_G
Cuts at: 513 1065 2004 2372 2783 3330 4422 513
Size: 552 939 368 411 547 1092 3991
Fragments arranged by size:
3991 1092 939 552 547 411 368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5110 487
Size: 683 56 3884 3277
Fragments arranged by size:
3884 3277 683 56
BtsI GCAGTG_nn'
Cuts at: 2576 3125 4067 4771 5416 6210 6230 2576
Size: 549 942 704 645 794 20 4246
Fragments arranged by size:
4246 942 794 704 645 549 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7900
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4088 4123 529
Size: 35 3524 35 4306
Fragments arranged by size:
4306 3524 35 35
DraI TTT'AAA
Cuts at: 5204 5204
Size: 7900
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3107 4442 6907 809
Size: 507 1791 1335 2465 1802
Fragments arranged by size:
2465 1802 1791 1335 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2680 3028 3456
Size: 54 1425 50 275 18 850 348 428
Cuts at: 3456 3523 4199 4245 5137 5347 6236 7865 8
Size: 67 676 46 892 210 889 1629 43
Fragments arranged by size:
1629 1425 892 889 850 676 428 348
275 210 67 54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2680 3523 4199 5347 1537
Size: 275 868 843 676 1148 4090
Fragments arranged by size:
4090 1148 868 843 676 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 3935 4138 4907 5830 1353
Size: 960 1622 203 769 923 3423
Fragments arranged by size:
3423 1622 960 923 769 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2018 2260 3330 3953 6487 6861
Size: 1064 138 429 242 1070 623 2534 374
Cuts at: 6861 7007 387
Size: 146 1280
Fragments arranged by size:
2534 1280 1070 1064 623 429 374 242
146 138
EcoICRI GAG'CTC
Cuts at: 2847 3055 3310 4578 2847
Size: 208 255 1268 6169
Fragments arranged by size:
6169 1268 255 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2303 2443 3065 3296 3521 3614
Size: 393 491 180 140 622 231 225 93
Cuts at: 3614 3832 4332 4523 4564 4731 5963 6560 7347
Size: 218 500 191 41 167 1232 597 787
Cuts at: 7347 7615 1239
Size: 268 1524
Fragments arranged by size:
1524 1232 787 622 597 500 491 393
268 231 225 218 191 180 167 140
93 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2976 3661 5195 2976
Size: 685 1534 5681
Fragments arranged by size:
5681 1534 685
EcoO109I rG'GnC_Cy
Cuts at: 2278 3009 3100 3335 3369 3382 3518 3532 4216
Size: 731 91 235 34 13 136 14 684
Cuts at: 4216 4531 5016 2278
Size: 315 485 5162
Fragments arranged by size:
5162 731 684 485 315 235 136 91
34 14 13
EcoRI G'AATT_C
Cuts at: 2471 5209 5587 2471
Size: 2738 378 4784
Fragments arranged by size:
4784 2738 378
FspI TGC'GCA
Cuts at: 2153 6406 2153
Size: 4253 3647
HaeII r_GCGC'y
Cuts at: 1678 2267 2495 2874 3577 7047 1678
Size: 589 228 379 703 3470 2531
Fragments arranged by size:
3470 2531 703 589 379 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 3041 3073 3668
Size: 32 135 32 962 32 475 32 595
Cuts at: 3668 3700 4310 4342 4640 4672 4810 4842 4976
Size: 32 610 32 298 32 138 32 134
Cuts at: 4976 5008 6541 6573 6615 6647 1373
Size: 32 1533 32 42 32 2626
Fragments arranged by size:
2626 1533 962 610 595 475 298 138
135 134 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3616 5244 5490 669
Size: 988 281 1678 1628 246 3079
Fragments arranged by size:
3079 1678 1628 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4894 748
Size: 4146 3754
HpaI GTT'AAC
Cuts at: 5490 5490
Size: 7900
KpnI G_GTAC'C
Cuts at: 4852 5225 4852
Size: 373 7527
MfeI C'AATT_G
Cuts at: 5499 5499
Size: 7900
MluI A'CGCG_T
Cuts at: 7776 7776
Size: 7900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3975 4458 4962 7014 7198 1305
Size: 2670 483 504 2052 184 2007
Fragments arranged by size:
2670 2052 2007 504 483 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3030 4247 10
Size: 54 1425 343 1198 1217 3663
Fragments arranged by size:
3663 1425 1217 1198 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3986 4401 5858 6217 6376 518
Size: 648 2820 415 1457 359 159 2042
Fragments arranged by size:
2820 2042 1457 648 415 359 159
MspA1I CmG'CkG
Cuts at: 1606 2374 2536 2617 2785 3126 3332 3499 3585
Size: 768 162 81 168 341 206 167 86
Cuts at: 3585 3735 4561 5053 5180 5316 5993 7141 7386
Size: 150 826 492 127 136 677 1148 245
Cuts at: 7386 1606
Size: 2120
Fragments arranged by size:
2120 1148 826 768 677 492 341 245
206 168 167 162 150 136 127 86
81
NaeI GCC'GGC
Cuts at: 3395 4279 3395
Size: 884 7016
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6409
Fragments arranged by size:
6409 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7900
NgoMIV G'CCGG_C
Cuts at: 3393 4277 3393
Size: 884 7016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7900
NotI GC'GGCC_GC
Cuts at: 5347 5347
Size: 7900
NspI r_CATG'y
Cuts at: 1132 1229 5258 6803 1132
Size: 97 4029 1545 2229
Fragments arranged by size:
4029 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3698 1348
Size: 2350 5550
PciI A'CATG_T
Cuts at: 6799 6799
Size: 7900
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 6274
PfoI T'CCnGG_A
Cuts at: 3312 4289 3312
Size: 977 6923
PmeI GTTT'AAAC
Cuts at: 5204 5204
Size: 7900
PmlI CAC'GTG
Cuts at: 2713 2713
Size: 7900
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5969 6001 7482 7514 3737
Size: 32 2200 32 1481 32 4123
Fragments arranged by size:
4123 2200 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3100 3382 3532 4216 5016 3100
Size: 282 150 684 800 5984
Fragments arranged by size:
5984 800 684 282 150
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7900
PsiI TTA'TAA
Cuts at: 5470 5470
Size: 7900
PspOMI G'GGCC_C
Cuts at: 2277 2391 3518 2277
Size: 114 1127 6659
Fragments arranged by size:
6659 1127 114
PspXI vC'TCGA_Gb
Cuts at: 2522 2899 2522
Size: 377 7523
PstI C_TGCA'G
Cuts at: 830 2541 2977 3435 3498 5252 830
Size: 1711 436 458 63 1754 3478
Fragments arranged by size:
3478 1754 1711 458 436 63
PvuI CG_AT'CG
Cuts at: 2001 4206 2001
Size: 2205 5695
PvuII CAG'CTG
Cuts at: 2536 3499 3585 5053 2536
Size: 963 86 1468 5383
Fragments arranged by size:
5383 1468 963 86
SacI G_AGCT'C
Cuts at: 2849 3057 3312 4580 2849
Size: 208 255 1268 6169
Fragments arranged by size:
6169 1268 255 208
SacII CC_GC'GG
Cuts at: 2375 2786 3333 2375
Size: 411 547 6942
Fragments arranged by size:
6942 547 411
SalI G'TCGA_C
Cuts at: 1936 5242 1936
Size: 3306 4594
SapI GCTCTTCn'nnn_
Cuts at: 3935 4907 3935
Size: 972 6928
SbfI CC_TGCA'GG
Cuts at: 5252 5252
Size: 7900
ScaI AGT'ACT
Cuts at: 6148 7860 6148
Size: 1712 6188
SfcI C'TryA_G
Cuts at: 826 1045 2358 2366 2537 2973 3431 3494 3651
Size: 219 1313 8 171 436 458 63 157
Cuts at: 3651 5248 6383 7064 7255 826
Size: 1597 1135 681 191 1471
Fragments arranged by size:
1597 1471 1313 1135 681 458 436 219
191 171 157 63 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7900
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7900
SmaI CCC'GGG
Cuts at: 1872 2765 2878 2999 3256 5227 1872
Size: 893 113 121 257 1971 4545
Fragments arranged by size:
4545 1971 893 257 121 113
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2094 2308 2522 2899 2960 6004
Size: 197 926 151 214 214 377 61 3044
Cuts at: 6004 6905 7167 820
Size: 901 262 1553
Fragments arranged by size:
3044 1553 926 901 377 262 214 214
197 151 61
SnaBI TAC'GTA
Cuts at: 493 5691 493
Size: 5198 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7900
SphI G_CATG'C
Cuts at: 1132 5258 1132
Size: 4126 3774
SspI AAT'ATT
Cuts at: 5 52 5824 5
Size: 47 5772 2081
Fragments arranged by size:
5772 2081 47
StuI AGG'CCT
Cuts at: 1517 2100 2651 2926 3752 3879 4462 1517
Size: 583 551 275 826 127 583 4955
Fragments arranged by size:
4955 826 583 583 551 275 127
StyI C'CwwG_G
Cuts at: 513 1065 2004 2646 2700 4212 4308 4527 5339
Size: 552 939 642 54 1512 96 219 812
Cuts at: 5339 513
Size: 3074
Fragments arranged by size:
3074 1512 939 812 642 552 219 96
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3922 4847 5927 5944 6097 6282 6727
Size: 1255 2342 925 1080 17 153 185 445
Cuts at: 6727 325
Size: 1498
Fragments arranged by size:
2342 1498 1255 1080 925 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2603 6146
Size: 274 80 33 51 1443 90 536 3543
Cuts at: 6146 7858 96
Size: 1712 138
Fragments arranged by size:
3543 1712 1443 536 274 138 90 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4041 4073 4936 4968 7860 7892 4041
Size: 32 863 32 2892 32 4049
Fragments arranged by size:
4049 2892 863 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7900
XbaI T'CTAG_A
Cuts at: 3064 5236 3064
Size: 2172 5728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3628 4240 3628
Size: 612 7288
XhoI C'TCGA_G
Cuts at: 1943 2094 2522 2899 1943
Size: 151 428 377 6944
Fragments arranged by size:
6944 428 377 151
XmaI C'CCGG_G
Cuts at: 1870 2763 2876 2997 3254 5225 1870
Size: 893 113 121 257 1971 4545
Fragments arranged by size:
4545 1971 893 257 121 113
XmnI GAAnn'nnTTC
Cuts at: 2208 3929 6029 2208
Size: 1721 2100 4079
Fragments arranged by size:
4079 2100 1721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4606 5708 276
Size: 53 83 186 936 3072 1102 2468
Fragments arranged by size:
3072 2468 1102 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI