Restriction map for
pFN21AA1419
(Circular) (Six-base) MAPSORT of: pFN21AA1419.seq Check: 1370 from: 1 to: 12001
pFN21AA1419 12001 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2176 2677 2995 3242 4888 2176
Size: 501 318 247 1646 9289
Fragments arranged by size:
9289 1646 501 318 247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4533 8847 9811 278
Size: 53 83 186 936 2997 4314 964 2468
Fragments arranged by size:
4314 2997 2468 964 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6488 8043 9344 1937
Size: 4551 1555 1301 4594
Fragments arranged by size:
4594 4551 1555 1301
Acc65I G'GTAC_C
Cuts at: 9322 9322
Size: 12001
AclI AA'CG_TT
Cuts at: 1563 10128 10501 1563
Size: 8565 373 3063
Fragments arranged by size:
8565 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 2542 3189 4074 5151 5207 5552 6545
Size: 509 401 647 885 1077 56 345 993
Cuts at: 6545 7724 8638 9183 10064 11448 11716 1632
Size: 1179 914 545 881 1384 268 1917
Fragments arranged by size:
1917 1384 1179 1077 993 914 885 881
647 545 509 401 345 268 56
AfeI AGC'GCT
Cuts at: 5270 8261 5270
Size: 2991 9010
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11804
AflIII A'CryG_T
Cuts at: 1203 7830 10900 11877 1203
Size: 6627 3070 977 1327
Fragments arranged by size:
6627 3070 1327 977
AgeI A'CCGG_T
Cuts at: 10832 10832
Size: 12001
AhdI GACnn_n'nnGTC
Cuts at: 6025 8419 10730 6025
Size: 2394 2311 7296
Fragments arranged by size:
7296 2394 2311
AleI CACnn'nnGTG
Cuts at: 1166 3356 1166
Size: 2190 9811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3075 4932 5165 6537 8450 9161 1184
Size: 1891 1857 233 1372 1913 711 4024
Fragments arranged by size:
4024 1913 1891 1857 1372 711 233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2243
Size: 32 11969
AlwNI CAG_nnn'CTG
Cuts at: 2191 2200 2503 5201 7048 7728 8186 8446 9744
Size: 9 303 2698 1847 680 458 260 1298
Cuts at: 9744 11316 2191
Size: 1572 2876
Fragments arranged by size:
2876 2698 1847 1572 1298 680 458 303
260 9
ApaI G_GGCC'C
Cuts at: 2105 2653 3365 2105
Size: 548 712 10741
Fragments arranged by size:
10741 712 548
ApaLI G'TGCA_C
Cuts at: 2057 10058 11214 2057
Size: 8001 1156 2844
Fragments arranged by size:
8001 2844 1156
ApoI r'AATT_y
Cuts at: 1493 2467 4210 4945 5965 7492 8068 9310 9536
Size: 974 1743 735 1020 1527 576 1242 226
Cuts at: 9536 9688 11797 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1743 1697 1527 1242 1020 974 735
576 226 152
AscI GG'CGCG_CC
Cuts at: 8986 8986
Size: 12001
AseI AT'TA_AT
Cuts at: 160 1032 7146 10555 160
Size: 872 6114 3409 1606
Fragments arranged by size:
6114 3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12001
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 3270 3593 3722 4422 4503 7768
Size: 73 151 1176 323 129 700 81 3265
Cuts at: 7768 8896 9120 9326 1870
Size: 1128 224 206 4545
Fragments arranged by size:
4545 3265 1176 1128 700 323 224 206
151 129 81 73
AvrII C'CTAG_G
Cuts at: 5716 5716
Size: 12001
BamHI G'GATC_C
Cuts at: 6842 9001 9331 9698 6842
Size: 2159 330 367 9145
Fragments arranged by size:
9145 2159 367 330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2672 2689 6158 9322 10777
Size: 325 217 627 885 17 3469 3164 1455
Cuts at: 10777 618
Size: 1842
Fragments arranged by size:
3469 3164 1842 1455 885 627 325 217
17
BanII G_rGCy'C
Cuts at: 1391 2105 2226 2653 3245 3365 3672 3791 4104
Size: 714 121 427 592 120 307 119 313
Cuts at: 4104 5521 7173 7741 8199 8537 9320 1391
Size: 1417 1652 568 458 338 783 4072
Fragments arranged by size:
4072 1652 1417 783 714 592 568 458
427 338 313 307 121 120 119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2412 3576 5195 6362 928
Size: 971 513 1164 1619 1167 6567
Fragments arranged by size:
6567 1619 1167 1164 971 513
BbvCI CC'TCA_GC
Cuts at: 2679 3092 3978 4826 6257 6695 7487 2679
Size: 413 886 848 1431 438 792 7193
Fragments arranged by size:
7193 1431 886 848 792 438 413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 10192 10226 1903
Size: 34 50 34 8171 34 3678
Fragments arranged by size:
8171 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2644 2809 3127 4425 4643 9893 11103 2644
Size: 165 318 1298 218 5250 1210 3542
Fragments arranged by size:
5250 3542 1298 1210 318 218 165
BclI T'GATC_A
Cuts at: 1548 5406 1548
Size: 3858 8143
BfrBI ATG'CAT
Cuts at: 7524 7524
Size: 12001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2670 3142
Size: 107 122 71 631 605 337 661 472
Cuts at: 3142 5399 6131 6807 8313 10612 136
Size: 2257 732 676 1506 2299 1525
Fragments arranged by size:
2299 2257 1525 1506 732 676 661 631
605 472 337 122 107 71
BglII A'GATC_T
Cuts at: 2271 8547 11996 2271
Size: 6276 3449 2276
Fragments arranged by size:
6276 3449 2276
BlpI GC'TnA_GC
Cuts at: 3785 6121 7062 9418 3785
Size: 2336 941 2356 6368
Fragments arranged by size:
6368 2356 2336 941
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2088 2105 2653 3365 3427 3594 3742
Size: 271 27 17 548 712 62 167 148
Cuts at: 3742 5688 5940 6161 10062 11218 1790
Size: 1946 252 221 3901 1156 2573
Fragments arranged by size:
3901 2573 1946 1156 712 548 271 252
221 167 148 62 27 17
BmgBI CAC'GTC
Cuts at: 1318 5073 1318
Size: 3755 8246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 2653 3570
Size: 416 53 83 399 24 876 360 917
Cuts at: 3570 4158 4367 5459 5586 7192 7336 8042 8328
Size: 588 209 1092 127 1606 144 706 286
Cuts at: 8328 9147 9732 10690 442
Size: 819 585 958 1753
Fragments arranged by size:
1753 1606 1092 958 917 876 819 706
588 585 416 399 360 286 209 144
127 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6107 6399 1056
Size: 5051 292 6658
Fragments arranged by size:
6658 5051 292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4096 4945 7318 7512 8984 4096
Size: 849 2373 194 1472 7113
Fragments arranged by size:
7113 2373 1472 849 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 3314 3350 3817 4372 5843 5886 6094
Size: 1082 993 36 467 555 1471 43 208
Cuts at: 6094 6583 7025 7231 7258 8843 8954 9202 10661
Size: 489 442 206 27 1585 111 248 1459
Cuts at: 10661 1239
Size: 2579
Fragments arranged by size:
2579 1585 1471 1459 1082 993 555 489
467 442 248 208 206 111 43 36
27
Bpu10I CC'TnA_GC
Cuts at: 2481 2679 3092 3790 3978 4826 5133 5549 6044
Size: 198 413 698 188 848 307 416 495
Cuts at: 6044 6257 6695 7167 7487 2481
Size: 213 438 472 320 6995
Fragments arranged by size:
6995 848 698 495 472 438 416 413
320 307 213 198 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 3377 3536 7701 10126 10991 11289 2519
Size: 858 159 4165 2425 865 298 3231
Fragments arranged by size:
4165 3231 2425 865 858 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4044 4721 7535 10664 882
Size: 617 2545 677 2814 3129 2219
Fragments arranged by size:
3129 2814 2545 2219 677 617
BsaAI yAC'GTr
Cuts at: 493 1204 2083 8530 9792 493
Size: 711 879 6447 1262 2702
Fragments arranged by size:
6447 2702 1262 879 711
BsaBI GATnn'nnATC
Cuts at: 11995 11995
Size: 12001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 3854 4392 4530
Size: 53 83 186 936 825 1496 538 138
Cuts at: 4530 4581 6039 8844 9808 10190 10825 275
Size: 51 1458 2805 964 382 635 1451
Fragments arranged by size:
2805 1496 1458 1451 964 936 825 635
538 382 186 138 83 53 51
BsaWI w'CCGG_w
Cuts at: 4626 5574 6617 6756 8131 10434 10832 11106 11253
Size: 948 1043 139 1375 2303 398 274 147
Cuts at: 11253 11664 4626
Size: 411 4963
Fragments arranged by size:
4963 2303 1375 1043 948 411 398 274
147 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3573 3603 3760 3790 4772
Size: 30 165 30 2170 30 157 30 982
Cuts at: 4772 4802 4835 4865 5284 5314 6005 6035 6046
Size: 30 33 30 419 30 691 30 11
Cuts at: 6046 6076 6695 6725 7229 7259 9095 9125 9227
Size: 30 619 30 504 30 1836 30 102
Cuts at: 9227 9257 1178
Size: 30 3922
Fragments arranged by size:
3922 2170 1836 982 691 619 504 419
165 157 102 33 30 30 30 30
30 30 30 30 30 30 30 30
30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2602 2947 3028 3338 3572 4843 4876 6166
Size: 1416 345 81 310 234 1271 33 1290
Cuts at: 6166 6298 7222 7427 8731 9271 1186
Size: 132 924 205 1304 540 3916
Fragments arranged by size:
3916 1416 1304 1290 1271 924 540 345
310 234 205 132 81 33
BseYI C'CCAG_C
Cuts at: 2106 3305 3742 3801 3813 3984 4103 4773 5324
Size: 1199 437 59 12 171 119 670 551
Cuts at: 5324 5618 5826 6116 6275 6501 6639 6972 7791
Size: 294 208 290 159 226 138 333 819
Cuts at: 7791 8309 11204 11700 2106
Size: 518 2895 496 2407
Fragments arranged by size:
2895 2407 1199 819 670 551 518 496
437 333 294 290 226 208 171 159
138 119 59 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 3081 4928 5457 1163
Size: 573 327 1018 1847 529 7707
Fragments arranged by size:
7707 1847 1018 573 529 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9451 10212 11240 1540
Size: 275 186 7450 761 1028 2301
Fragments arranged by size:
7450 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 3791 7173 7998 8286 8537 10062 10147 11218
Size: 1730 3382 825 288 251 1525 85 1071
Cuts at: 11218 2061
Size: 2844
Fragments arranged by size:
3382 2844 1730 1525 1071 825 288 251
85
BsmI GAATG_Cn'
Cuts at: 7526 7859 9512 9605 7526
Size: 333 1653 93 9922
Fragments arranged by size:
9922 1653 333 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2327 4383 4537 4755 7249 8272 2327
Size: 2056 154 218 2494 1023 6056
Fragments arranged by size:
6056 2494 2056 1023 218 154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2088 2105 2226 2653 3245 3365
Size: 399 271 27 17 121 427 592 120
Cuts at: 3365 3427 3594 3672 3742 3791 4104 5521 5688
Size: 62 167 78 70 49 313 1417 167
Cuts at: 5688 5940 6161 7173 7741 7998 8199 8286 8537
Size: 252 221 1012 568 257 201 87 251
Cuts at: 8537 9320 10062 10147 11218 1391
Size: 783 742 85 1071 2174
Fragments arranged by size:
2174 1417 1071 1012 783 742 592 568
427 399 313 271 257 252 251 221
201 167 167 121 120 87 85 78
70 62 49 27 17
BspEI T'CCGG_A
Cuts at: 4626 6617 6756 11664 4626
Size: 1991 139 4908 4963
Fragments arranged by size:
4963 4908 1991 139
BspHI T'CATG_A
Cuts at: 9890 11620 9890
Size: 1730 10271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2176 2677 2995 3242 4428 4888 5427 8643
Size: 1332 501 318 247 1186 460 539 3216
Cuts at: 8643 9356 844
Size: 713 3489
Fragments arranged by size:
3489 3216 1332 1186 713 539 501 460
318 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2241 3003 3289 3766 9888 1394
Size: 429 418 762 286 477 6122 3507
Fragments arranged by size:
6122 3507 762 477 429 418 286
BsrDI GCAATG_nn'
Cuts at: 65 1248 3399 3463 4414 10496 10670 65
Size: 1183 2151 64 951 6082 174 1396
Fragments arranged by size:
6082 2151 1396 1183 951 174 64
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 3181 4695 5393 8340 10645 10832
Size: 110 198 1173 1514 698 2947 2305 187
Cuts at: 10832 1700
Size: 2869
Fragments arranged by size:
2947 2869 2305 1514 1173 698 198 187
110
BsrGI T'GTAC_A
Cuts at: 96 4032 96
Size: 3936 8065
BssHII G'CGCG_C
Cuts at: 1925 8986 1925
Size: 7061 4940
BssSI C'ACGA_G
Cuts at: 2927 9056 10061 11073 2927
Size: 6129 1005 1012 3855
Fragments arranged by size:
6129 3855 1012 1005
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2191 2200 2689 7728 794
Size: 1397 9 489 5039 5067
Fragments arranged by size:
5067 5039 1397 489 9
BstBI TT'CG_AA
Cuts at: 3312 10805 3312
Size: 7493 4508
BstEII G'GTnAC_C
Cuts at: 1184 6011 9167 1184
Size: 4827 3156 4018
Fragments arranged by size:
4827 4018 3156
BstXI CCAn_nnnn'nTGG
Cuts at: 6775 8887 8978 6775
Size: 2112 91 9798
Fragments arranged by size:
9798 2112 91
BstYI r'GATC_y
Cuts at: 1972 2169 2271 2332 2432 4156 6842 8547 9001
Size: 197 102 61 100 1724 2686 1705 454
Cuts at: 9001 9331 9698 10083 10100 11552 11638 11650 11996
Size: 330 367 385 17 1452 86 12 346
Cuts at: 11996 1972
Size: 1977
Fragments arranged by size:
2686 1977 1724 1705 1452 454 385 367
346 330 197 102 100 86 61 17
12
BstZ17I GTA'TAC
Cuts at: 6489 6489
Size: 12001
Bsu36I CC'TnA_GG
Cuts at: 3147 4161 8714 9087 3147
Size: 1014 4553 373 6061
Fragments arranged by size:
6061 4553 1014 373
BtgI C'CryG_G
Cuts at: 513 1065 3111 3357 3672 4014 4293 7779 9032
Size: 552 2046 246 315 342 279 3486 1253
Cuts at: 9032 513
Size: 3482
Fragments arranged by size:
3486 3482 2046 1253 552 342 315 279
246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4177 4614 5926 6286 487
Size: 683 56 2951 437 1312 360 6202
Fragments arranged by size:
6202 2951 1312 683 437 360 56
BtsI GCAGTG_nn'
Cuts at: 4008 5302 6040 6055 6268 7191 7481 7729 9517
Size: 1294 738 15 213 923 290 248 1788
Cuts at: 9517 10311 10331 4008
Size: 794 20 5678
Fragments arranged by size:
5678 1788 1294 923 794 738 290 248
213 20 15
ClaI AT'CG_AT
Cuts at: 1554 8883 1554
Size: 7329 4672
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11966
DraI TTT'AAA
Cuts at: 9305 9305
Size: 12001
DraIII CAC_nnn'GTG
Cuts at: 6997 8530 6997
Size: 1533 10468
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2781 4286 6364 11008 809
Size: 507 1465 1505 2078 4644 1802
Fragments arranged by size:
4644 2078 1802 1505 1465 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2311 2643
Size: 54 1425 50 275 18 180 301 332
Cuts at: 2643 3042 4306 5400 8334 8704 9448 10337 11966
Size: 399 1264 1094 2934 370 744 889 1629
Cuts at: 11966 8
Size: 43
Fragments arranged by size:
2934 1629 1425 1264 1094 889 744 399
370 332 301 275 180 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 9448 1537
Size: 275 7636 4090
Fragments arranged by size:
7636 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 2508 2558 3309 3434 3707 3847 6388
Size: 878 277 50 751 125 273 140 2541
Cuts at: 6388 6779 8626 8870 9931 1353
Size: 391 1847 244 1061 3423
Fragments arranged by size:
3423 2541 1847 1061 878 751 391 277
273 244 140 125 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 5047 5377 10588 10962 11108
Size: 1064 138 2876 582 330 5211 374 146
Cuts at: 11108 387
Size: 1280
Fragments arranged by size:
5211 2876 1280 1064 582 374 330 146
138
EcoICRI GAG'CTC
Cuts at: 3789 7171 8535 3789
Size: 3382 1364 7255
Fragments arranged by size:
7255 3382 1364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2321 2542 3189 3314 3350 3817
Size: 393 509 180 221 647 125 36 467
Cuts at: 3817 4074 4372 5151 5207 5552 5843 5886 6094
Size: 257 298 779 56 345 291 43 208
Cuts at: 6094 6545 6583 7025 7231 7258 7724 8638 8843
Size: 451 38 442 206 27 466 914 205
Cuts at: 8843 8954 9183 9202 10064 10661 11448 11716 1239
Size: 111 229 19 862 597 787 268 1524
Fragments arranged by size:
1524 914 862 787 779 647 597 509
467 466 451 442 393 345 298 291
268 257 229 221 208 206 205 180
125 111 56 43 38 36 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3554 6474 7768 3554
Size: 2920 1294 7787
Fragments arranged by size:
7787 2920 1294
EcoO109I rG'GnC_Cy
Cuts at: 2102 2649 2650 2783 3151 3267 3362 3511 6271
Size: 547 1 133 368 116 95 149 2760
Cuts at: 6271 2102
Size: 7832
Fragments arranged by size:
7832 2760 547 368 149 133 116 95
1
EcoRI G'AATT_C
Cuts at: 4945 7492 8068 9310 9688 4945
Size: 2547 576 1242 378 7258
Fragments arranged by size:
7258 2547 1242 576 378
EcoRV GAT'ATC
Cuts at: 7597 7597
Size: 12001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5258 5258
Size: 12001
FspI TGC'GCA
Cuts at: 8756 10507 8756
Size: 1751 10250
HaeII r_GCGC'y
Cuts at: 1678 2067 3749 5272 8263 11148 1678
Size: 389 1682 1523 2991 2885 2531
Fragments arranged by size:
2991 2885 2531 1682 1523 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2175 4382 4414 4659
Size: 32 135 32 571 32 2207 32 245
Cuts at: 4659 4691 4772 4804 4973 5005 5020 5052 5873
Size: 32 81 32 169 32 15 32 821
Cuts at: 5873 5905 6011 6043 6046 6078 6834 6866 7229
Size: 32 106 32 3 32 756 32 363
Cuts at: 7229 7261 7929 7961 8405 8437 8958 8990 9095
Size: 32 668 32 444 32 521 32 105
Cuts at: 9095 9127 9227 9259 10642 10674 10716 10748 1373
Size: 32 100 32 1383 32 42 32 2626
Fragments arranged by size:
2626 2207 1383 821 756 668 571 521
444 363 245 169 135 106 105 100
81 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 15 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 5743 7818 8044 8382 9345
Size: 988 281 900 2905 2075 226 338 963
Cuts at: 9345 9591 669
Size: 246 3079
Fragments arranged by size:
3079 2905 2075 988 963 900 338 281
246 226
HindIII A'AGCT_T
Cuts at: 748 7359 748
Size: 6611 5390
HpaI GTT'AAC
Cuts at: 9591 9591
Size: 12001
KpnI G_GTAC'C
Cuts at: 9326 9326
Size: 12001
MfeI C'AATT_G
Cuts at: 9600 9600
Size: 12001
MluI A'CGCG_T
Cuts at: 11877 11877
Size: 12001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5477 5625 5676 6165 6705 7527 8140
Size: 3780 392 148 51 489 540 822 613
Cuts at: 8140 8270 8475 9270 11115 11299 1305
Size: 130 205 795 1845 184 2007
Fragments arranged by size:
3780 2007 1845 822 795 613 540 489
392 205 184 148 130 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5402 8336 10
Size: 54 1425 343 3570 2934 3675
Fragments arranged by size:
3675 3570 2934 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3356 4298 4574 5006 5147 6668 8180
Size: 648 2190 942 276 432 141 1521 1512
Cuts at: 8180 8885 9031 9188 9264 9959 10318 10477 518
Size: 705 146 157 76 695 359 159 2042
Fragments arranged by size:
2190 2042 1521 1512 942 705 695 648
432 359 276 159 157 146 141 76
MspA1I CmG'CkG
Cuts at: 1606 2213 2230 2804 3484 3587 3817 4495 4773
Size: 607 17 574 680 103 230 678 278
Cuts at: 4773 4800 4998 5418 5951 6234 6572 6976 7061
Size: 27 198 420 533 283 338 404 85
Cuts at: 7061 7130 7630 8313 9175 9417 10094 11242 11487
Size: 69 500 683 862 242 677 1148 245
Cuts at: 11487 1606
Size: 2120
Fragments arranged by size:
2120 1148 862 683 680 678 677 607
574 533 500 420 404 338 283 278
245 242 230 198 103 85 69 27
17
NaeI GCC'GGC
Cuts at: 2010 3183 5395 2010
Size: 1173 2212 8616
Fragments arranged by size:
8616 2212 1173
NcoI C'CATG_G
Cuts at: 513 1065 3672 7779 9032 513
Size: 552 2607 4107 1253 3482
Fragments arranged by size:
4107 3482 2607 1253 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12001
NgoMIV G'CCGG_C
Cuts at: 2008 3181 5393 2008
Size: 1173 2212 8616
Fragments arranged by size:
8616 2212 1173
NheI G'CTAG_C
Cuts at: 1052 6103 6395 1052
Size: 5051 292 6658
Fragments arranged by size:
6658 5051 292
NotI GC'GGCC_GC
Cuts at: 9448 9448
Size: 12001
NsiI A_TGCA'T
Cuts at: 7526 7526
Size: 12001
NspI r_CATG'y
Cuts at: 1132 1229 3349 3721 7834 7886 8497 9187 9359
Size: 97 2120 372 4113 52 611 690 172
Cuts at: 9359 10904 1132
Size: 1545 2229
Fragments arranged by size:
4113 2229 2120 1545 690 611 372 172
97 52
PasI CC'CwG_GG
Cuts at: 1348 4750 8994 1348
Size: 3402 4244 4355
Fragments arranged by size:
4355 4244 3402
PciI A'CATG_T
Cuts at: 7830 10900 7830
Size: 3070 8931
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 3075 5201 7630 8799 1108
Size: 1278 689 2126 2429 1169 4310
Fragments arranged by size:
4310 2429 2126 1278 1169 689
PfoI T'CCnGG_A
Cuts at: 4349 5555 6816 7641 8511 8820 8946 4349
Size: 1206 1261 825 870 309 126 7404
Fragments arranged by size:
7404 1261 1206 870 825 309 126
PmeI GTTT'AAAC
Cuts at: 9305 9305
Size: 12001
PmlI CAC'GTG
Cuts at: 2083 8530 2083
Size: 6447 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10070 10102 11583 11615 10070
Size: 32 1481 32 10456
Fragments arranged by size:
10456 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2783 3267 3511 6271 2783
Size: 484 244 2760 8513
Fragments arranged by size:
8513 2760 484 244
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12001
PsiI TTA'TAA
Cuts at: 9571 9571
Size: 12001
PspOMI G'GGCC_C
Cuts at: 2101 2649 3361 2101
Size: 548 712 10741
Fragments arranged by size:
10741 712 548
PspXI vC'TCGA_Gb
Cuts at: 4422 4422
Size: 12001
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4234 4266 4234
Size: 32 11969
PstI C_TGCA'G
Cuts at: 830 2196 2205 2512 2809 6413 6553 7207 7733
Size: 1366 9 307 297 3604 140 654 526
Cuts at: 7733 8259 8640 9078 9201 9353 830
Size: 526 381 438 123 152 3478
Fragments arranged by size:
3604 3478 1366 654 526 526 438 381
307 297 152 140 123 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12001
PvuII CAG'CTG
Cuts at: 3587 3817 4495 4773 5951 6234 6976 7061 7130
Size: 230 678 278 1178 283 742 85 69
Cuts at: 7130 7630 3587
Size: 500 7958
Fragments arranged by size:
7958 1178 742 678 500 283 278 230
85 69
RsrII CG'GwC_CG
Cuts at: 4629 4629
Size: 12001
SacI G_AGCT'C
Cuts at: 3791 7173 8537 3791
Size: 3382 1364 7255
Fragments arranged by size:
7255 3382 1364
SalI G'TCGA_C
Cuts at: 1936 8042 9343 1936
Size: 6106 1301 4594
Fragments arranged by size:
6106 4594 1301
SanDI GG'GwC_CC
Cuts at: 3267 6271 3267
Size: 3004 8997
SapI GCTCTTCn'nnn_
Cuts at: 2231 3707 6779 8870 2231
Size: 1476 3072 2091 5362
Fragments arranged by size:
5362 3072 2091 1476
SbfI CC_TGCA'GG
Cuts at: 9353 9353
Size: 12001
ScaI AGT'ACT
Cuts at: 6418 10249 11961 6418
Size: 3831 1712 6458
Fragments arranged by size:
6458 3831 1712
SexAI A'CCwGG_T
Cuts at: 5731 5731
Size: 12001
SfcI C'TryA_G
Cuts at: 826 1045 2192 2201 2508 2805 6070 6409 6549
Size: 219 1147 9 307 297 3265 339 140
Cuts at: 6549 7203 7729 8089 8255 8636 9074 9197 9349
Size: 654 526 360 166 381 438 123 152
Cuts at: 9349 10484 11165 11356 826
Size: 1135 681 191 1471
Fragments arranged by size:
3265 1471 1147 1135 681 654 526 438
381 360 339 307 297 219 191 166
152 140 123 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12001
SmaI CCC'GGG
Cuts at: 1872 2096 3272 3724 4505 9328 1872
Size: 224 1176 452 781 4823 4545
Fragments arranged by size:
4823 4545 1176 781 452 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2534 3392 3551 4422 7716 8896
Size: 197 926 591 858 159 871 3294 1180
Cuts at: 8896 10105 11006 11268 820
Size: 1209 901 262 1553
Fragments arranged by size:
3294 1553 1209 1180 926 901 871 858
591 262 197 159
SnaBI TAC'GTA
Cuts at: 493 9792 493
Size: 9299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12001
SphI G_CATG'C
Cuts at: 1132 3349 3721 9359 1132
Size: 2217 372 5638 3774
Fragments arranged by size:
5638 3774 2217 372
SrfI GCCC'GGGC
Cuts at: 2096 2096
Size: 12001
SspI AAT'ATT
Cuts at: 5 52 9925 5
Size: 47 9873 2081
Fragments arranged by size:
9873 2081 47
StuI AGG'CCT
Cuts at: 1517 2550 5676 6558 1517
Size: 1033 3126 882 6960
Fragments arranged by size:
6960 3126 1033 882
StyI C'CwwG_G
Cuts at: 513 1065 2994 3074 3672 3753 5153 5716 6312
Size: 552 1929 80 598 81 1400 563 596
Cuts at: 6312 6855 7779 8701 8793 9032 9440 513
Size: 543 924 922 92 239 408 3074
Fragments arranged by size:
3074 1929 1400 924 922 598 596 563
552 543 408 239 92 81 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3118 3953 4646 5475 6680 6986 8650
Size: 1255 1538 835 693 829 1205 306 1664
Cuts at: 8650 9004 10028 10045 10198 10383 10828 325
Size: 354 1024 17 153 185 445 1498
Fragments arranged by size:
1664 1538 1498 1255 1205 1024 835 829
693 445 354 306 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4032 4470 6416
Size: 274 80 33 51 1443 2055 438 1946
Cuts at: 6416 10247 11959 96
Size: 3831 1712 138
Fragments arranged by size:
3831 2055 1946 1712 1443 438 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 3573 3605 3760 3792 3821 3853 5284
Size: 32 1420 32 155 32 29 32 1431
Cuts at: 5284 5316 6272 6304 6695 6727 7448 7480 7651
Size: 32 956 32 391 32 721 32 171
Cuts at: 7651 7683 7904 7936 11961 11993 2121
Size: 32 221 32 4025 32 2129
Fragments arranged by size:
4025 2129 1431 1420 956 721 391 221
171 155 32 32 32 32 32 32
32 32 32 32 32 29
Tth111I GACn'n_nGTC
Cuts at: 1316 7241 1316
Size: 5925 6076
XbaI T'CTAG_A
Cuts at: 9337 9337
Size: 12001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2666 3917 5022 8407 9016 2666
Size: 1251 1105 3385 609 5651
Fragments arranged by size:
5651 3385 1251 1105 609
XhoI C'TCGA_G
Cuts at: 1943 4422 8896 1943
Size: 2479 4474 5048
Fragments arranged by size:
5048 4474 2479
XmaI C'CCGG_G
Cuts at: 1870 2094 3270 3722 4503 9326 1870
Size: 224 1176 452 781 4823 4545
Fragments arranged by size:
4823 4545 1176 781 452 224
XmnI GAAnn'nnTTC
Cuts at: 3224 5242 6834 8696 10130 3224
Size: 2018 1592 1862 1434 5095
Fragments arranged by size:
5095 2018 1862 1592 1434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4531 8845 9809 276
Size: 53 83 186 936 2997 4314 964 2468
Fragments arranged by size:
4314 2997 2468 964 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
BaeI BbeI BsiWI FseI FspAI KasI NarI NruI
PacI SacII SfiI SfoI SwaI