Restriction map for
pFN21AA1417
(Circular) (Six-base) MAPSORT of: pFN21AA1417.seq Check: 4965 from: 1 to: 8764
pFN21AA1417 8764 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2536 2536
Size: 8764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6574 278
Size: 53 83 186 936 5038 2468
Fragments arranged by size:
5038 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5398 6107 1937
Size: 3461 709 4594
Fragments arranged by size:
4594 3461 709
Acc65I G'GTAC_C
Cuts at: 5744 6085 5744
Size: 341 8423
AclI AA'CG_TT
Cuts at: 1563 4969 6891 7264 1563
Size: 3406 1922 373 3063
Fragments arranged by size:
3406 3063 1922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2760 3305 3786 4174 4295 4643 5115
Size: 437 691 545 481 388 121 348 472
Cuts at: 5115 5802 6012 6827 8211 8479 1632
Size: 687 210 815 1384 268 1917
Fragments arranged by size:
1917 1384 815 691 687 545 481 472
437 388 348 268 210 121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8567
AflIII A'CryG_T
Cuts at: 1203 3882 7663 8640 1203
Size: 2679 3781 977 1327
Fragments arranged by size:
3781 2679 1327 977
AgeI A'CCGG_T
Cuts at: 7595 7595
Size: 8764
AhdI GACnn_n'nnGTC
Cuts at: 2254 7493 2254
Size: 5239 3525
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4188 1184
Size: 3004 5760
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4910 4942 4910
Size: 32 8732
AlwNI CAG_nnn'CTG
Cuts at: 5093 5825 6507 8079 5093
Size: 732 682 1572 5778
Fragments arranged by size:
5778 1572 732 682
ApaLI G'TGCA_C
Cuts at: 6821 7977 6821
Size: 1156 7608
ApoI r'AATT_y
Cuts at: 1493 2458 2561 3219 3351 3504 3615 3806 3997
Size: 965 103 658 132 153 111 191 191
Cuts at: 3997 4018 4181 4340 4646 5704 6073 6299 6451
Size: 21 163 159 306 1058 369 226 152
Cuts at: 6451 8560 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1058 965 658 369 306 226
191 191 163 159 153 152 132 111
103 21
AseI AT'TA_AT
Cuts at: 160 1032 4242 7318 160
Size: 872 3210 3076 1606
Fragments arranged by size:
3210 3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
AvaI C'yCGr_G
Cuts at: 1870 1943 6089 1870
Size: 73 4146 4545
Fragments arranged by size:
4545 4146 73
BamHI G'GATC_C
Cuts at: 6094 6461 6094
Size: 367 8397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3846 5713 5744 6085 7540
Size: 325 217 627 2059 1867 31 341 1455
Cuts at: 7540 618
Size: 1842
Fragments arranged by size:
2059 1867 1842 1455 627 341 325 217
31
BanII G_rGCy'C
Cuts at: 1391 6083 1391
Size: 4692 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3310 928
Size: 971 1411 6382
Fragments arranged by size:
6382 1411 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6955 6989 1903
Size: 34 5018 34 3678
Fragments arranged by size:
5018 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6656 7866 6656
Size: 1210 7554
BclI T'GATC_A
Cuts at: 1548 4558 1548
Size: 3010 5754
BfrBI ATG'CAT
Cuts at: 5576 5576
Size: 8764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7375 136
Size: 107 122 71 631 605 5703 1525
Fragments arranged by size:
5703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8759 8759
Size: 8764
BlpI GC'TnA_GC
Cuts at: 6181 6181
Size: 8764
Bme1580I G_kGCm'C
Cuts at: 1790 6825 7981 1790
Size: 5035 1156 2573
Fragments arranged by size:
5035 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3311 4312 1318
Size: 1993 1001 5770
Fragments arranged by size:
5770 1993 1001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2954 5283 5840
Size: 416 53 83 399 24 1537 2329 557
Cuts at: 5840 6495 7453 442
Size: 655 958 1753
Fragments arranged by size:
2329 1753 1537 958 655 557 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 2506 2640 3283 5004 7424 1239
Size: 862 405 134 643 1721 2420 2579
Fragments arranged by size:
2579 2420 1721 862 643 405 134
Bpu10I CC'TnA_GC
Cuts at: 2715 2715
Size: 8764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3533 6889 7754 8052 3533
Size: 3356 865 298 4245
Fragments arranged by size:
4245 3356 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2319 3004 4740 7427 882
Size: 617 820 685 1736 2687 2219
Fragments arranged by size:
2687 2219 1736 820 685 617
BsaAI yAC'GTr
Cuts at: 493 1204 4964 6555 493
Size: 711 3760 1591 2702
Fragments arranged by size:
3760 2702 1591 711
BsaBI GATnn'nnATC
Cuts at: 2537 8758 2537
Size: 6221 2543
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6571 6953 7588 275
Size: 53 83 186 936 5038 382 635 1451
Fragments arranged by size:
5038 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4785 7197 7595 7869 8016 8427 4785
Size: 2412 398 274 147 411 5122
Fragments arranged by size:
5122 2412 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3045 3075 1178
Size: 30 165 30 1642 30 6867
Fragments arranged by size:
6867 1642 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2979 5954 1186
Size: 1793 2975 3996
Fragments arranged by size:
3996 2975 1793
BseYI C'CCAG_C
Cuts at: 7967 8463 7967
Size: 496 8268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 3717 4449 1163
Size: 573 275 1706 732 5478
Fragments arranged by size:
5478 1706 732 573 275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6214 6975 8003 1540
Size: 275 186 4213 761 1028 2301
Fragments arranged by size:
4213 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6825 6910 7981 6825
Size: 85 1071 7608
Fragments arranged by size:
7608 1071 85
BsmI GAATG_Cn'
Cuts at: 2170 3225 3690 3757 4633 5578 5593 6275 6368
Size: 1055 465 67 876 945 15 682 93
Cuts at: 6368 2170
Size: 4566
Fragments arranged by size:
4566 1055 945 876 682 465 93 67
15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 6083 6825 6910 7981 1391
Size: 399 4293 742 85 1071 2174
Fragments arranged by size:
4293 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 8427 8427
Size: 8764
BspHI T'CATG_A
Cuts at: 3694 6653 8383 3694
Size: 2959 1730 4075
Fragments arranged by size:
4075 2959 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2536 2584 2871 6119 844
Size: 1692 48 287 3248 3489
Fragments arranged by size:
3489 3248 1692 287 48
BsrBI CCG'CTC
Cuts at: 1394 1823 6651 1394
Size: 429 4828 3507
Fragments arranged by size:
4828 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2270 3021 3240 4662 4809 7259 7433
Size: 1183 1022 751 219 1422 147 2450 174
Cuts at: 7433 65
Size: 1396
Fragments arranged by size:
2450 1422 1396 1183 1022 751 219 174
147
BsrFI r'CCGG_y
Cuts at: 1700 1810 7408 7595 1700
Size: 110 5598 187 2869
Fragments arranged by size:
5598 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8764
BssSI C'ACGA_G
Cuts at: 6824 7836 6824
Size: 1012 7752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2551 794
Size: 1757 7007
BstBI TT'CG_AA
Cuts at: 7568 7568
Size: 8764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8764
BstXI CCAn_nnnn'nTGG
Cuts at: 5059 5059
Size: 8764
BstYI r'GATC_y
Cuts at: 1972 2233 2374 2512 2959 3556 3592 4768 5365
Size: 261 141 138 447 597 36 1176 597
Cuts at: 5365 6094 6461 6846 6863 8315 8401 8413 8759
Size: 729 367 385 17 1452 86 12 346
Cuts at: 8759 1972
Size: 1977
Fragments arranged by size:
1977 1452 1176 729 597 597 447 385
367 346 261 141 138 86 36 17
12
BtgI C'CryG_G
Cuts at: 513 1065 5240 5740 5835 513
Size: 552 4175 500 95 3442
Fragments arranged by size:
4175 3442 552 500 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4767 487
Size: 683 56 3541 4484
Fragments arranged by size:
4484 3541 683 56
BtsI GCAGTG_nn'
Cuts at: 3296 3759 6280 7074 7094 3296
Size: 463 2521 794 20 4966
Fragments arranged by size:
4966 2521 794 463 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8729
DraI TTT'AAA
Cuts at: 3339 5235 5692 6068 3339
Size: 1896 457 376 6035
Fragments arranged by size:
6035 1896 457 376
DraIII CAC_nnn'GTG
Cuts at: 2572 2572
Size: 8764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2685 7771 809
Size: 507 1369 5086 1802
Fragments arranged by size:
5086 1802 1369 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6211 7100 8729
Size: 54 1425 50 275 18 4381 889 1629
Cuts at: 8729 8
Size: 43
Fragments arranged by size:
4381 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6211 1537
Size: 275 4399 4090
Fragments arranged by size:
4399 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3396 3642 3731 4094 4807 5073 5988 6694
Size: 2043 246 89 363 713 266 915 706
Cuts at: 6694 1353
Size: 3423
Fragments arranged by size:
3423 2043 915 713 706 363 266 246
89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7351 7725 7871 387
Size: 1064 138 5762 374 146 1280
Fragments arranged by size:
5762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2101 2506 2640 2760 3283 3305
Size: 393 437 32 405 134 120 523 22
Cuts at: 3305 3786 4174 4295 4643 5004 5115 5802 6012
Size: 481 388 121 348 361 111 687 210
Cuts at: 6012 6827 7424 8211 8479 1239
Size: 815 597 787 268 1524
Fragments arranged by size:
1524 815 787 687 597 523 481 437
405 393 388 361 348 268 210 134
121 120 111 32 22
EcoRI G'AATT_C
Cuts at: 3504 3997 6073 6451 3504
Size: 493 2076 378 5817
Fragments arranged by size:
5817 2076 493 378
EcoRV GAT'ATC
Cuts at: 4876 4876
Size: 8764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5912 5912
Size: 8764
FspI TGC'GCA
Cuts at: 7270 7270
Size: 8764
HaeII r_GCGC'y
Cuts at: 1678 7911 1678
Size: 6233 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2240 2272 2504
Size: 32 135 32 443 32 193 32 232
Cuts at: 2504 2536 3045 3077 3422 3454 5120 5152 7405
Size: 32 509 32 345 32 1666 32 2253
Cuts at: 7405 7437 7479 7511 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2253 1666 509 443 345 232 193
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3274 5082 6108 6354 669
Size: 988 281 1336 1808 1026 246 3079
Fragments arranged by size:
3079 1808 1336 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8764
HpaI GTT'AAC
Cuts at: 6354 6354
Size: 8764
KpnI G_GTAC'C
Cuts at: 5748 6089 5748
Size: 341 8423
MfeI C'AATT_G
Cuts at: 6363 6363
Size: 8764
MluI A'CGCG_T
Cuts at: 8640 8640
Size: 8764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7878 8062 1305
Size: 6573 184 2007
Fragments arranged by size:
6573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6942
Fragments arranged by size:
6942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2035 2640 4029 4303 4356 6722 7081
Size: 648 869 605 1389 274 53 2366 359
Cuts at: 7081 7240 518
Size: 159 2042
Fragments arranged by size:
2366 2042 1389 869 648 605 359 274
159 53
MspA1I CmG'CkG
Cuts at: 1606 2711 3623 6180 6857 8005 8250 1606
Size: 1105 912 2557 677 1148 245 2120
Fragments arranged by size:
2557 2120 1148 1105 912 677 245
NcoI C'CATG_G
Cuts at: 513 1065 5240 5740 5835 513
Size: 552 4175 500 95 3442
Fragments arranged by size:
4175 3442 552 500 95
NdeI CA'TA_TG
Cuts at: 387 3168 387
Size: 2781 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8764
NotI GC'GGCC_GC
Cuts at: 6211 6211
Size: 8764
NsiI A_TGCA'T
Cuts at: 5578 5578
Size: 8764
NspI r_CATG'y
Cuts at: 1132 1229 2057 2655 3886 5948 6122 7667 1132
Size: 97 828 598 1231 2062 174 1545 2229
Fragments arranged by size:
2229 2062 1545 1231 828 598 174 97
PasI CC'CwG_GG
Cuts at: 1348 2467 1348
Size: 1119 7645
PciI A'CATG_T
Cuts at: 3882 7663 3882
Size: 3781 4983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5805 1108
Size: 4697 4067
PfoI T'CCnGG_A
Cuts at: 2522 2617 5616 2522
Size: 95 2999 5670
Fragments arranged by size:
5670 2999 95
PmeI GTTT'AAAC
Cuts at: 6068 6068
Size: 8764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6833 6865 8346 8378 6833
Size: 32 1481 32 7219
Fragments arranged by size:
7219 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8764
PsiI TTA'TAA
Cuts at: 4992 6334 4992
Size: 1342 7422
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3855 3887 3855
Size: 32 8732
PstI C_TGCA'G
Cuts at: 830 3588 3844 6116 830
Size: 2758 256 2272 3478
Fragments arranged by size:
3478 2758 2272 256
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8764
PvuII CAG'CTG
Cuts at: 2711 3623 2711
Size: 912 7852
SalI G'TCGA_C
Cuts at: 1936 6106 1936
Size: 4170 4594
SapI GCTCTTCn'nnn_
Cuts at: 4094 4094
Size: 8764
SbfI CC_TGCA'GG
Cuts at: 6116 6116
Size: 8764
ScaI AGT'ACT
Cuts at: 7012 8724 7012
Size: 1712 7052
SfcI C'TryA_G
Cuts at: 826 1045 2427 3057 3584 3840 5025 6112 7247
Size: 219 1382 630 527 256 1185 1087 1135
Cuts at: 7247 7928 8119 826
Size: 681 191 1471
Fragments arranged by size:
1471 1382 1185 1135 1087 681 630 527
256 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8764
SmaI CCC'GGG
Cuts at: 1872 6091 1872
Size: 4219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3512 6868 7769 8031 820
Size: 197 926 1569 3356 901 262 1553
Fragments arranged by size:
3356 1569 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6555 493
Size: 6062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8764
SphI G_CATG'C
Cuts at: 1132 2057 5948 6122 1132
Size: 925 3891 174 3774
Fragments arranged by size:
3891 3774 925 174
SspI AAT'ATT
Cuts at: 5 52 4135 4382 6688 5
Size: 47 4083 247 2306 2081
Fragments arranged by size:
4083 2306 2081 247 47
StuI AGG'CCT
Cuts at: 1517 2108 1517
Size: 591 8173
StyI C'CwwG_G
Cuts at: 513 1065 5240 5499 5740 5835 6203 513
Size: 552 4175 259 241 95 368 3074
Fragments arranged by size:
4175 3074 552 368 259 241 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 6791 6808 6961 7146 7591 325
Size: 1255 449 4762 17 153 185 445 1498
Fragments arranged by size:
4762 1498 1255 449 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7010 8722 96
Size: 274 80 33 51 1443 5033 1712 138
Fragments arranged by size:
5033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2165 2197 8724 8756 2165
Size: 32 6527 32 2173
Fragments arranged by size:
6527 2173 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8764
XbaI T'CTAG_A
Cuts at: 5788 6100 5788
Size: 312 8452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4324 5574 4324
Size: 1250 7514
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8764
XmaI C'CCGG_G
Cuts at: 1870 6089 1870
Size: 4219 4545
XmnI GAAnn'nnTTC
Cuts at: 3415 3504 4383 5067 6893 3415
Size: 89 879 684 1826 5286
Fragments arranged by size:
5286 1826 879 684 89
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6572 276
Size: 53 83 186 936 5038 2468
Fragments arranged by size:
5038 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI